Publications by authors named "Yan Yuan"

527 Publications

Research progress on the mechanism of orexin in pain regulation in different brain regions.

Open Life Sci 2021 20;16(1):46-52. Epub 2021 Jan 20.

Department of Anesthesiology, The Affiliated Hospital of Xuzhou Medical University, No. 84 Huaihai West Road, Quanshan District, Xuzhou, Jiangsu Province 221002, People's Republic of China.

Orexin is a neuropeptide that is primarily synthesized and secreted by the lateral hypothalamus (LH) and includes two substances derived from the same precursor (orexin A [OXA] and orexin B [OXB]). Studies have shown that orexin is not only involved in the regulation of eating, the sleep-wake cycle, and energy metabolism, but also closely associated with various physiological functions, such as cardiovascular control, reproduction, stress, reward, addiction, and the modulation of pain transmission. At present, studies that have been performed both domestically and abroad have confirmed that orexin and its receptors are closely associated with pain regulation. In this article, the research progress on acute pain regulation involving orexin is reviewed.
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http://dx.doi.org/10.1515/biol-2021-0001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874592PMC
January 2021

Behavior of Nutritional Supplements Use in Association With Inflammatory Skin Diseases in Chinese College Students.

Front Nutr 2021 17;8:615462. Epub 2021 Mar 17.

Mobile Joint Laboratory, Xiangya Hospital, Central South University, Changsha, China.

It is understudied how frequently adolescents use nutritional supplements (NS) and whether the corresponding behavior is associated with skin diseases that may cause unpleasant symptoms and disfigurement. The current study aimed to investigate the prevalence of NS use in Chinese college students and its association with inflammatory skin diseases. This was a university-based epidemiologic investigation that included 20,138 students who underwent dermatological examinations. A questionnaire survey was conducted to inquire about the use of NS along with related information. Skin diseases were diagnosed by dermatologists during the health examination. Logistic regression models were used for analysis. Adjusted odds ratios (aORs) were presented as the effect size. Survey responses from a total of 20,138 participants were analyzed. Specifically, 18.3% of the participants reported the use of NS in the past year. The use of vitamin C was most frequently reported, accounting for a proportion of 12.9%, followed by vitamin B and mineral supplements. The use of NS was found to be associated with female sex, Han ethnicity, higher annual household income, and a series of healthy lifestyles such as more physical activity, less second-hand smoke exposure, less alcohol consumption, and higher intake of milk and yogurt ( < 0.001). Participants with chronic urticaria (aOR = 1.3; 95% CI, 1.0-1.7), atopic dermatitis (aOR = 1.4; 95% CI, 1.2-1.6), or acne (aOR = 1.17; 95% CI, 1.04-1.31) were more likely to use NS, especially herbs (aOR = 2.7; 95% CI, 1.2-3.7), followed by vitamin B (aOR = 1.6; 95% CI, 1.2-2.0) and mineral supplements (aOR = 1.4; 95% CI, 1.0-2.0). College students with inflammatory skin diseases are more likely to use NS.
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http://dx.doi.org/10.3389/fnut.2021.615462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010123PMC
March 2021

Progesterone decreased indoleamine 2,3-dioxygenase 1(IDO1) expression in early pregnancy chorionic villi and decidua.

Autoimmunity 2021 Apr 1:1-7. Epub 2021 Apr 1.

Guizhou Provincial People's Hospital, Guiyang, China.

Indoleamine 2,3-dioxygenase1(IDO1) is one of the most important proteins in protect the embryos from the mother's immune system during pregnancy. However, the regulation of the protein expression at the maternal-foetal interface is not fully known. We aimed to study the regulation of IDO1 expression by progesterone in villi and decidua of in early pregnancy. Fifty cases of early pregnancy women's villi and decidua were collected. Tissue explants of chorionic villi and the decidua were cultured in media containing in different concentrations of progesterone, in the presence or absence of mifepristone. Western blot analysis and immunofluorescence were used to detect the expression of IDO1 in chorionic villi and decidua in cultured tissues. IDO1 protein was identified in chorionic villi and decidua tissues of normal pregnant women, and the expression of IDO1in the decidua was significantly higher than those in chorionic villi. Progesterone decreased IDO1 expression in early pregnancy chorionic villi and decidua, and mifepristone, as the progesterone inhibitor, reverted this effect. In normal physiological state of pregnancy, progesterone may be involved in the regulation of immune tolerance by negative regulation of IDO1 expression at maternal foetal interface. Progesterone may down-regulate IDO1 expression during early pregnancy.
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http://dx.doi.org/10.1080/08916934.2021.1907572DOI Listing
April 2021

Europium coordination polymer particles based electrospun nanofibrous film for point-of-care testing of copper (II) ions.

Talanta 2021 Jun 4;228:122270. Epub 2021 Mar 4.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, PR China.

Excess free copper in serum has been identified to induce neurodegenerative diseases such as Alzheimer's disease, thus it is very important to determine copper (II) ions (Cu) content for human health test. Herein we developed a point-of-care testing (POCT) platform through a luminescence "on-off" recognition mechanism of serum copper. Microsized europium coordination polymer particles (Eu-CPs), which was prepared with citric acid (CA) and europium nitrate hexahydrate through a hydrothermal route, were then successfully loaded with the mixture of 2,6-pyridinedicarboxylic acid (DPA) and poly(vinyl alcohol) (PVA) to form electrospun nanofibrous films (ENFFs). The as-prepared Eu-CPs/DPA/PVA ENFFs exhibited red emission at 618 nm when exciting at 280 nm, with the quantum yields of 22.2% owing to the antenna effect from DPA to Eu. Furthermore, the strong luminescence could be selectively quenched by Cu through coordination with DPA to interrupt the antenna effect. With that, Cu was successfully detected in the range of 2-45 μM with a detection of limit of 1.3 μM, well matching with the requirement of clinic test of excess free copper induced neurodegenerative diseases. As a proof of concept at last, this POCT platform was used to detect free copper in spiked serum samples with a recovery of 101.1%-105.2%, demonstrating that this platform provides significant potential for use in clinical test.
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http://dx.doi.org/10.1016/j.talanta.2021.122270DOI Listing
June 2021

Rapid implementation of a cohort for the study of post-acute sequelae of SARS-CoV-2 infection/COVID-19.

medRxiv 2021 Mar 12. Epub 2021 Mar 12.

Background: As the coronavirus disease 2019 (COVID-19) pandemic continues and millions remain vulnerable to infection with severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), attention has turned to characterizing post-acute sequelae of SARS-CoV-2 infection (PASC).

Methods: From April 21 to December 31, 2020, we assembled a cohort of consecutive volunteers who a) had documented history of SARS-CoV-2 RNA-positivity; b) were ≥ 2 weeks past onset of COVID-19 symptoms or, if asymptomatic, first test for SARS-CoV-2; and c) were able to travel to our site in San Francisco. Participants learned about the study by being identified on medical center-based registries and being notified or by responding to advertisements. At 4-month intervals, we asked participants about physical symptoms that were new or worse compared to the period prior to COVID-19, mental health symptoms and quality of life. We described 4 time periods: 1) acute illness (0-3 weeks), 2) early recovery (3-10 weeks), 3) late recovery 1 (12-20 weeks), and 4) late recovery 2 (28-36 weeks). Blood and oral specimens were collected at each visit.

Results: We have, to date, enrolled 179 adults. During acute SARS-CoV-2 infection, 10 had been asymptomatic, 125 symptomatic but not hospitalized, and 44 symptomatic and hospitalized. In the acute phase, the most common symptoms were fatigue, fever, myalgia, cough and anosmia/dysgeusia. During the post-acute phase, fatigue, shortness of breath, concentration problems, headaches, trouble sleeping and anosmia/dysgeusia were the most commonly reported symptoms, but a variety of others were endorsed by at least some participants. Some experienced symptoms of depression, anxiety, and post-traumatic stress, as well as difficulties with ambulation and performance of usual activities. The median visual analogue scale value rating of general health was lower at 4 and 8 months (80, interquartile range [IQR]: 70-90; and 80, IQR 75-90) compared to prior to COVID-19 (85; IQR 75-90). Biospecimens were collected at nearly 600 participant-visits.

Conclusion: Among a cohort of participants enrolled in the post-acute phase of SARS-CoV-2 infection, we found many with persistent physical symptoms through 8 months following onset of COVID-19 with an impact on self-rated overall health. The presence of participants with and without symptoms and ample biological specimens will facilitate study of PASC pathogenesis. Similar evaluations in a population-representative sample will be needed to estimate the population-level prevalence of PASC.
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http://dx.doi.org/10.1101/2021.03.11.21252311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987054PMC
March 2021

Joint artifact correction and super-resolution of image slicing and mapping system via a convolutional neural network.

Opt Express 2021 Mar;29(5):7247-7260

As the key component of the image mapping spectrometer, the image mapper introduces complex image degradation in the reconstructed images, including low spatial resolution and intensity artifacts. In this paper, we propose a novel image processing method based on the convolutional neural network to perform artifact correction and super-resolution (SR) simultaneously. The proposed joint network contains two branches to handle the artifact correction task and SR task in parallel. The artifact correction module is designed to remove the artifacts in the image and the SR module is used to improve the spatial resolution. An attention fusion module is constructed to combine the features extracted by the artifact correction and SR modules. The fused features are used to reconstruct an artifact-free high-resolution image. We present extensive simulation results to demonstrate that the proposed joint method outperforms state-of-the-art methods and can be generalized to other image mapper designs. We also provide experimental results to prove the efficiency of the joint network.
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http://dx.doi.org/10.1364/OE.413076DOI Listing
March 2021

Cadmium exposure induces rat proximal tubular cells injury via p62-dependent Nrf2 nucleus translocation mediated activation of AMPK/AKT/mTOR pathway.

Ecotoxicol Environ Saf 2021 May 11;214:112058. Epub 2021 Mar 11.

College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou 225009, People's Republic of China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, People's Republic of China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, People's Republic of China. Electronic address:

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a nuclear transcription factor of great concern which is widely involved in physiological and pathological processes of the organism, but the role and regulatory mechanism of Nrf2 in kidney exposed to cadmium (Cd) remain largely unknown. Here we demonstrated that Cd exposure induced injury in primary rat proximal tubular (rPT) cells and NRK-52E cell line, which was accompanied by autophagic flux blockade and subsequent accumulation of p62. Cd-activated nucleus translocation of Nrf2 depended on p62, which promoted antioxidant genes transcription, but it failed to against Cd-induced cell injury and ultimately succumbed to Cd toxicity. CDDO Methyl Ester (CDDO-ME) or ML385 treatment aggravated or alleviated rPT cells injury induced by Cd respectively, indicating that Nrf2 nucleus translocation played a negative role during Cd-induced rPT cells injury. Phosphorylation of 5' AMP-activated protein kinase (AMPK) decreased together with enhanced Nrf2 nucleus translocation in rPT cells exposed to Cd. Dephosphorylation of AMPK induced by Cd were facilitated or restored by CDDO-ME or ML385 treatment, which confirmed AMPK is a downstream factor of Nrf2. Simultaneously, CDDO-ME further enhanced Phosphorylation of mTOR and AKT which increased during Cd exposure. While, Cd-induced phosphorylation of mTOR and AKT were reversed by ML385 treatment. These results illustrated that Cd mediated Nrf2 nucleus translocation depends on p62 accumulation which results from autophagic flux inhibition. The enhanced nucleus translocation of Nrf2 suppresses phosphorylation of AMPK to inactivate AKT/mTOR signaling, and results in rPT cells injury finally.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112058DOI Listing
May 2021

Central monitoring in a randomized, open-label, controlled phase 3 clinical trial for a treatment-shortening regimen for pulmonary tuberculosis.

Contemp Clin Trials 2021 Mar 10;104:106355. Epub 2021 Mar 10.

U.S. Centers for Disease Control & Prevention, Atlanta, GA, United States of America.

Introduction: With the growing use of online study management systems and rapid availability of data, timely data review and quality assessments are necessary to ensure proper clinical trial implementation. In this report we describe central monitoring used to ensure protocol compliance and accurate data reporting, implemented during a large phase 3 clinical trial.

Material And Methods: The Tuberculosis Trials Consortium (TBTC) Study 31/AIDS Clinical Trials Group (ACTG) study A5349 (S31) is an international, multi-site, randomized, open-label, controlled, non-inferiority phase 3 clinical trial comparing two 4-month regimens to a standard 6 month regimen for treatment of drug-susceptible tuberculosis (TB) among adolescents and adults with a sample size of 2500 participants.

Results: Central monitoring utilized primary study data in a five-tiered approach, including (1) real-time data checks & topic-specific intervention reports, (2) missing forms reports, (3) quality assurance metrics, (4) critical data reports and (5) protocol deviation identification, aimed to detect and resolve quality challenges. Over the course of the study, 240 data checks and reports were programed across the five tiers used.

Discussion: This use of primary study data to identify issues rapidly allowed the study sponsor to focus quality assurance and data cleaning activities on prioritized data, related to protocol compliance and accurate reporting of study results. Our approach enabled us to become more efficient and effective as we informed sites about deviations, resolved missing or inconsistent data, provided targeted guidance, and gained a deeper understanding of challenges experienced at clinical trial sites.

Trial Registration: This trial was registered with ClinicalTrials.gov (Identifier: NCT02410772) on April 8, 2015.
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http://dx.doi.org/10.1016/j.cct.2021.106355DOI Listing
March 2021

Alpha-Lipoic Acid Attenuates Cadmium- and Lead-Induced Neurotoxicity by Inhibiting Both Endoplasmic-Reticulum Stress and Activation of Fas/FasL and Mitochondrial Apoptotic Pathways in Rat Cerebral Cortex.

Neurotox Res 2021 Mar 10. Epub 2021 Mar 10.

College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.

Although many studies have reported toxic effects of cadmium (Cd) and lead (Pb) in the central nervous system, few studies have investigated the combined toxicity of Cd and Pb. The mechanisms by which these combined heavy metals induce toxicity, as well as effective means to exert neuroprotection from these agents, remain poorly understood. To investigate the protective effects of alpha-lipoic acid (α-LA) on Cd- and/or Pb-induced cortical damage in rats, 48 Sprague-Dawley rats were exposed to drinking water containing 50 mg/L of Cd and/or 300 mg/L of Pb for 12 weeks, in the presence or absence of α-LA co-treatment (50 mg/kg) via gavage. We observed that exposure to Cd and/or Pb decreased the brain weight/body weight ratio and increased Cd and/or Pb contents as well as ultrastructural damage to the cerebral cortex. Cd and/or Pb also induced endoplasmic-reticulum (ER) stress and activated Fas (CD95/APO-1)/Fas ligand (FasL) and mitochondrial apoptotic pathways. Furthermore, co-treatment of Cd and Pb further exacerbated part of these phenotypes than treatment of Cd or Pb alone. However, simultaneous supplementation with α-LA attenuated Cd and/or Pb-induced neurotoxicity by increasing the brain weight/body weight ratio, reducing Cd and/or Pb contents, ameliorating both nuclear/mitochondrial damage and ER stress, and attenuating activation of Fas/FasL and mitochondrial apoptotic pathways. Collectively, our results indicate that the accumulation of Cd and/or Pb causes cortical damage and that α-LA exerts protection against Cd- and/or Pb-induced neurotoxicity. These findings highlight that α-LA may be exploited for the treatment and prevention of Cd- and/or Pb-induced neurotoxicity.
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http://dx.doi.org/10.1007/s12640-021-00348-8DOI Listing
March 2021

A new method for in-situ treatment of waste gas scrubbing liquid containing both NH and HS based on sulfur autotrophic denitrification and partial nitrification-Anammox coupling system.

Bioresour Technol 2021 Jun 1;329:124925. Epub 2021 Mar 1.

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou 215009, China; Jiangsu Cooperative Innovation Center of Water Treatment Technology and Materials, Suzhou University of Science and Technology, Suzhou 215009, China.

In this study, an integrated device with scrubbing and biochemical treatment functions was used, and partial nitrification (PN)-Anammox and sulfur autotrophic denitrification (SADN) processes were coupled in a biochemical treatment pond to explore the feasibility of in-situ autotrophic removal of NH and HS. The results showed that the removal efficiency of NH and HS in waste gas are 95% and 87.5% respectively. The scrubbing liquid was efficiently treated in the biochemical treatment pond. Nitrogenous compounds weren't accumulated in liquid and converted to N by SADN and PN-Anammox coupling system. S was mainly used by SADN process to reduce NO. The scrubbing liquid processed by the biochemical treatment pond was refluxed to the scrubber to achieve continuous absorption of NH and HS. Microbial community and functional microbial analysis showed that the PN-Anammox and SADN processes were the main processes to achieve the conversion of pollutants in the scrubbing liquid.
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http://dx.doi.org/10.1016/j.biortech.2021.124925DOI Listing
June 2021

Kaposi's sarcoma herpesvirus is associated with osteosarcoma in Xinjiang populations.

Proc Natl Acad Sci U S A 2021 Mar;118(10)

Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong 510080, China;

Osteosarcoma is the most common malignant tumor of bone predominately affecting adolescents and young adults. Based on animal studies, a viral etiology of osteosarcoma was proposed more than a half-century ago, but no viral association with human osteosarcoma has been found. The Uyghur ethnic population in Xinjiang, China, has an unusually high prevalence of Kaposi's sarcoma-associated herpesvirus (KSHV) infection and elevated incidence of osteosarcoma. In the current study, we explored the possible association of KSHV infection and osteosarcoma occurrence. Our seroepidemiological study revealed that KSHV prevalence was significantly elevated in Uyghur osteosarcoma patients versus the general Uyghur population (OR, 10.23; 95%CI, 4.25, 18.89). The KSHV DNA genome and viral latent nuclear antigen LANA were detected in most osteosarcoma tumor cells. Gene expression profiling analysis showed that KSHV-positive osteosarcoma represents a distinct subtype of osteosarcomas with viral gene-activated signaling pathways important for osteosarcoma development. We conclude that KSHV infection is a risk factor for osteosarcoma, and KSHV is associated with some osteosarcomas, representing a newly identified viral-associated endemic cancer.
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http://dx.doi.org/10.1073/pnas.2016653118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958238PMC
March 2021

Corrigendum to "TGF-β-activated kinase 1 (TAK1) mediates cadmium-induced autophagy in osteoblasts via the AMPK / mTORC1 / ULK1 pathway" [Toxicology 442 (2020) 152538].

Toxicology 2021 Apr 2;453:152738. Epub 2021 Mar 2.

College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, 225009, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, PR China. Electronic address:

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http://dx.doi.org/10.1016/j.tox.2021.152738DOI Listing
April 2021

Community-driven research in the canadian arctic: dietary exposure to methylmercury and gastric health outcomes.

Int J Circumpolar Health 2021 Dec;80(1):1889879

Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada.

Indigenous Arctic Canadians have a higher prevalence of gastric neoplasms relative to North Americans of European ancestry. We investigated the hypothesis that low-dose methylmercury exposure from eating fish/whale increases the risk of gastric cancer in Arctic communities. We used intermediate endpoints from an established model of gastric carcinogenesis: intestinal metaplasia, atrophy, and severe chronic gastritis. During 2008-2012, we obtained gastric biopsies from participants of community-driven projects in 3 communities. In 2016, we collected hair samples to measure methylmercury levels and interviewed them about diet. In cross-sectional analysis, logistic regression estimated odds ratios for the estimated effect of hair-methylmercury concentration on the prevalence of each pathology outcome stratified by selenium intake. Among 80 participants, prevalence of intestinal metaplasia, atrophy and severe chronic gastritis was 17, 29 and 38%, respectively. Adjusted Odds of severe chronic gastritis and atrophy were highest at hair-methylmercury concentrations ≥1μg/g when estimated selenium intake was 0, and approached 0 for all methylmercury levels as estimated selenium intake increased. Gastric pathology increased with methylmercury exposure when selenium intake was low. Though limited by small numbers, these findings suggest selenium ingested by eating fish/whale may counter harmful effects of methylmercury exposure in Arctic populations.
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http://dx.doi.org/10.1080/22423982.2021.1889879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928021PMC
December 2021

Chirality transfer of cysteine to the plasmonic resonance region through silver coating of gold nanobipyramids.

Chem Commun (Camb) 2021 Apr 1;57(26):3211-3214. Epub 2021 Mar 1.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.

Here we report a chirality transfer of cysteine, which at first was to the plasmonic resonance region of gold nanobipyramids and then to that of Ag nanoshells with increasing growth of Ag layers, owing to the important role of the free Cys embedded within the Ag nanoshell. Our finding is helpful for developing new chiral plasmonic nanomaterials with the best designed asymmetric properties.
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http://dx.doi.org/10.1039/d0cc07576kDOI Listing
April 2021

Impact of metabolism-related mutations on the heart rate of gastric cancer patients after peritoneal lavage.

World J Clin Cases 2021 Feb;9(6):1318-1328

Department of Cardio-Thoracic Surgery, The Third Affiliated Hospital of Soochow University, Changzhou 213003, Jiangsu Province, China.

Background: During surgery for gastric cancer, peritoneal lavage using warm distilled water can cause temporary hemodynamic changes.

Aim: To examine the associations between changes in heart rate and single nucleotide polymorphisms (SNPs).

Methods: This was a prospective observational study of patients with gastric cancer who underwent gastrectomy and peritoneal hypotonic lavage at the Third Affiliated Hospital of Soochow University from March 2018 to March 2019. Related SNPs were selected, and the verified exons were analyzed. Heart rate and blood pressure (BP) were measured before and after lavage. The patients were grouped as heart rate change ≥ 30% < 30%. Comparison and regression analyses of the selected SNPs were performed between the two groups.

Results: According to the inclusion/exclusion criteria, 194 patients were included in the analysis. Of these patients, 138 were male, with a mean age of 65.9 ± 0.8 years, and 56 were female, with a mean age of 65.0 ± 1.3 years. Heart rate dropped by 0%-10% in 65 participants, by 10%-15% in 29, by 15%-20% in 23, by 20%-50% in 39, by 50%-100% in four, six had a cardiac arrest, and 28 had an increase in heart rate. Considering the possible impact of exonic SNPs on the phenotypes, TEP1 (rs938886), TEP1 (rs1713449), and RECQL5 (rs820196) were analyzed. The haplotype analysis suggested that the haplotypes CTT [odds ratio (OR) = 2.018, 95% confidence interval (CI): 1.012-4.025, = 0.0430] and GCC (OR = 2.293, 95%CI: 1.174-4.477, = 0.0131) of TEP1 (rs938886), TEP1 (rs1713449), and RECQL5 (rs820196) increased the risk of a drop in heart rate > 30%.

Conclusion: The TEP1 (rs938886), TEP1 (rs1713449), and RECQL5 (rs820196) SNPs were associated with changes in heart rate ≥ 30% during intraperitoneal lavage using distilled water after gastrectomy for gastric cancer.
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http://dx.doi.org/10.12998/wjcc.v9.i6.1318DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896693PMC
February 2021

Cadmium Toxicity on Chondrocytes and the Palliative Effects of 1α, 25-Dihydroxy Vitamin D in White Leghorns Chicken's Embryo.

Front Vet Sci 2021 10;8:637369. Epub 2021 Feb 10.

College of Veterinary Medicine, Yangzhou University, Yangzhou, China.

Cadmium (Cd) can causes osteoporosis and joint swelling. However, the mechanism of Cd toxicity in chondrocytes and how to alleviate Cd poisoning to chondrocytes are still unclear. Herein, we evaluated the toxicity of Cd to chicken chondrocytes, and whether vitamin D can relieve the toxicity of Cd to chondrocytes. Primary chondrocytes were collected from knee-joint cartilage of 15-day-old chicken embryos. They were treated with (0, 1, 2, and 4) μM Cd alone, 10 M 1α,25-(OH)D alone, or 2 μM Cd combined with 10 M 1α,25-(OH)D. We found that Cd significantly inhibited and mRNA expression, which are markers for chondrocyte differentiation, downregulated the mitochondrial membrane potential, upregulated the Bax/B-cell lymphoma 2 ratio. Furthermore, Cd significantly promoted matrix metalloproteinase (MMP)-9 expression, thus accelerating the degradation of extracellular matrix. And Cd also inhibited the expression of main macromolecular protein of extracellular matrix, Collagen type IIα1 (COL2A1) and acid mucopolysaccharide. However, 1α,25-(OH)D pretreatment significantly alleviated the toxicity effects of Cd on the differentiation, apoptosis and extracellular matrix gene expression in primary chondrocytes. Conclusively, Cd exposure could inhibited chicken embryo chondrocytes differentiation, extracellular matrix gene expression, and induced chondrocyte apoptosis. However, these toxic effects of Cd are alleviated by the pretreatment of chondrocytes with 1α,25-(OH)D.
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http://dx.doi.org/10.3389/fvets.2021.637369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902530PMC
February 2021

Influence of seasonal temperature change on autotrophic nitrogen removal for mature landfill leachate treatment with high-ammonia by partial nitrification-Anammox process.

J Environ Sci (China) 2021 Apr 13;102:291-300. Epub 2020 Oct 13.

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou 215009, China.

In this study, a denitrification (DN)-partial nitritation (PN)-anaerobic ammonia oxidation (Anammox) system for the efficient nitrogen removal of mature landfill leachate was built with a zone-partitioning self-reflux biological reactor as the core device, and the effects of changes in seasonal temperature on the nitrogen removal in non-temperature-control environment were explored. The results showed that as the seasonal temperature decreased from 34°C to 11.3°C, the total nitrogen removal rate of the DN-PN-Anammox system gradually decreased from the peak value of 1.42 kg/(m•day) to 0.49 kg/(m•day). At low temperatures (<20°C), when the nitrogen load (NLR) of the system is not appropriate, the fluctuation of high NH-N concentration in the landfill leachate greatly influenced the stability of the nitrogen removal. At temperatures of 11°C-15°C, the NLR of the system is controlled below 0.5 kg/(m•day), which can achieve stable nitrogen removal and the nitrogen removal efficiency can reach above 96%. The abundance of Candidatus Brocadia gradually increased with the decrease of temperature. Nitrosomonas, Candidatus Brocadia and Candidatus Kuenenia as the main functional microorganisms in the low temperature.
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http://dx.doi.org/10.1016/j.jes.2020.09.031DOI Listing
April 2021

Neoadjuvant chemoradiotherapy for patients with unresectable radically locally advanced colon cancer: a potential improvement to overall survival and decrease to multivisceral resection.

BMC Cancer 2021 Feb 19;21(1):179. Epub 2021 Feb 19.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

Background: The management of unresectable locally advanced colon cancer (LACC) remains controversial, as resection is not feasible. The goal of this study was to evaluate the treatment outcomes and toxicity of neoadjuvant chemoradiotherapy (NACRT) followed with surgery and adjuvant chemotherapy in patients with unresectable radically LACC.

Methods: We included patients who were diagnosed at our institution, 2010-2018. The neoadjuvant regimen consisted of radiotherapy and capecitabine/ 5-fluorouracil-based chemotherapy.

Results: One hundred patients were identified. The median follow-up time was 32 months. The R0 resection rate, adjusted nonmultivisceral resection rate and bladder preservation rate were 83.0, 43.0 and 83.3%, respectively. The pCR and clinical-downstaging rates were 18, and 81.0%%, respectively. The 3-year PFS and OS rates for all patients were 68.6 and 82.1%, respectively. Seventeen patients developed grade 3-4 myelosuppression, which was the most common adverse event observed after NACRT. Tumor perforation occurred in 3 patients during NACRT. The incidence of grade 3-4 surgery-related complications was 7.0%. Postoperative anastomotic leakage was observed in 3 patients.

Conclusions: NACRT followed by surgery was feasible and safe for selected patients with LACC, and can be used as a conversion treatment to achieve satisfactory downstaging, long-term survival and quality of life, with acceptable toxicities.
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http://dx.doi.org/10.1186/s12885-021-07894-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893883PMC
February 2021

Elevated Circulating Levels of Carnal ENST00000550337.1 Are Associated with Polycystic Ovary Syndrome in Chinese Women.

Gynecol Obstet Invest 2021 Feb 18:1-7. Epub 2021 Feb 18.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Objectives: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disease. Some studies reported that the development of PCOS may be closely related to insulin resistance (IR). Interestingly, the long noncoding RNA (lncRNA) ENST00000550337.1 in peripheral blood is mainly involved in glucose metabolism. Therefore, the purpose of our study was to explore the relationship between lncRNA ENST00000550337.1 level and PCOS patients.

Materials And Methods: Seventy-five PCOS patients and 72 healthy controls were enrolled in this study. We used qRT-PCR to detect the expression level of lncRNA ENST00000550337.1 in peripheral blood leukocytes from patients with PCOS. We also investigated potential relationships between lncRNA ENST00000550337.1 and the endocrine parameters in PCOS.

Results: We observed that the expression of lncRNA ENST00000550337.1 in PCOS patients was significantly higher than that in the control subjects and positively correlated with PCOS occurrence, waist circumference, waist-hip ratio, IR, fasting insulin levels, and blood glucose. The expression of lnc RNA ENST00000550337.1 was positively correlated with PCOS (p = 0.003). There were independent correlations between IR and expression of lncRNA ENST00000550337.1 in patients with PCOS. Patients with elevated lncRNA ENST00000550337.1 expression had significantly increased PCOS risk after adjusting for age and BMI. LncRNA ENST00000550337.1 expression level provided a sensitivity of 81.3% and a specificity of 78.1% with a threshold value of 6.4648 for the prediction of PCOS. The area under the ROC was 0.813.

Limitations: There are some limitations to this study. First, the sample size was limited and the causal relationship between lncRNA ENST00000550337.1 and PCOS was not investigated due to the cross-sectional study design. Second, HOMA-IR does not fully accurately reflect the IR of patients.

Conclusions: The present study indicated that lnc RNA ENST00000550337.1 was related to PCOS occurrence, and elevated levels may be a risk factor for PCOS women. In addition, lncRNA ENST00000550337.1 might promote PCOS development partially by increasing IR and can be used as a potential molecular marker in patients with PCOS.
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http://dx.doi.org/10.1159/000513671DOI Listing
February 2021

Nucleolin-Targeted DNA Nanotube for Precise Cancer Therapy through Förster Resonance Energy Transfer-Indicated Telomerase Responsiveness.

Anal Chem 2021 02 10;93(7):3526-3534. Epub 2021 Feb 10.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P. R. China.

Precise drug delivery holds great promise in cancer treatment but still faces challenges in controllable drug release in tumor cells specifically. Herein, a nucleolin-targeted and telomerase-responsive DNA nanotube for drug release was developed. First, a DNA nanosheet with four capture strands on its surface was prepared, which could bind and load ricin A chain (RTA). The RTA-loaded nanosheet was further converted into a DNA nanotube with a high Förster resonance energy transfer (FRET) efficiency in the presence of a Cy3-modified DNA fastener by hybridizing with the Cy5-modified DNA and another DNA-containing telomerase primer sequence along the long sides. Moreover, the aptamer of nucleolin was assembled on the DNA nanotube by combining with the hybrid chain at the terminal. The aptamer-functionalized and RTA-loaded DNA nanotube displayed enhanced tumor permeability and precise drug release in response to the telomerase in tumor cells, following the change of the FRET signal and RTA-induced cell death. Moreover, the DNA nanotube was applied successfully in vivo, and there was an obvious inhibition of tumor growth on xenograft-bearing mice following systemic administration, indicating that the constructed DNA nanotube represents a promising platform for precise RTA delivery in target cancer therapy.
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http://dx.doi.org/10.1021/acs.analchem.0c04917DOI Listing
February 2021

Zearalenone and deoxynivalenol inhibited IL-4 receptor-mediated Th2 cell differentiation and aggravated bacterial infection in mice.

Toxicol Appl Pharmacol 2021 Mar 5;415:115441. Epub 2021 Feb 5.

College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, Jiangsu, China. Electronic address:

The immunotoxicity of zearalenone (ZEA) and deoxynivalenol (DON), two of the most common environmental mycotoxins, has been well investigated. However, due to the complexity of the immune system, especially during bacterial infection, many types of immune cells are involved in invasion resistance and bacterial clearance. Of these, T helper 2 (Th2) cells, which are members of the helper T cell family, assist B cells to activate and differentiate into antibody-secreting cells, participate in humoral immune response, and, ultimately, eliminate pathogens. Thus, it is important to identify the stage at which these toxins affect the immune function, and to clarity the underlying mechanisms. In this study, mice infected with Listeria monocytogenes (Listeria) were used to study the effects of ZEA, DON, and ZEA + DON on Th2 differentiation, Interleukin-4 Receptor (IL-4R) expression, costimulatory molecules expression and cytokine secretion after Listeria infection. Naive CD4 T cells, isolated from mice, were used to verify the in vivo effects and the associated mechanisms. In vivo experiments showed that these toxins aggravated spleen damage after Listeria infection and reduced the differentiation of Th2 cells by affecting the synthesis of IL-4R of CD4 T cells. In addition, the level of the costimulatory molecule CD154 decreased. Consistent with this, in vitro studies showed that these toxins inhibited the differentiation of mouse naive CD4 T cell into Th2 subtype and decreased IL-4R levels. In addition, the levels of costimulatory molecules CD154, CD278 and the Th2 cells secrete cytokines IL-4, IL-6, and IL-10 decreased. Based on our in vivo and in vitro experiments, we suggest that ZEA, DON, and ZEA + DON inhibit the expression of costimulatory molecules on CD4 T cell, and inhibit the IL-4R-mediated Th2 cell differentiation. This may indicate that the body cannot normally resist or clear the pathogen after mycotoxin poisoning.
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http://dx.doi.org/10.1016/j.taap.2021.115441DOI Listing
March 2021

CFD Analysis of a Water Vaporization Process in a Three-Dimensional Spouted Bed for Flue Gas Desulfurization.

ACS Omega 2021 Feb 22;6(4):2759-2766. Epub 2021 Jan 22.

School of Chemical Engineering, Northwest University, Xi'an 710069, China.

In this work, the gas-solid flow and water vaporization process are simulated by the method of Euler-Eulerian two-fluid model in a three-dimensional spouted bed, which have a significant influence on the desulfurization efficiency. The results of simulation indicate that the change trends of the particle volume fraction are similar under superficial gas velocities of 0.7 and 0.8 m/s. The degree of particle pulsation is the highest at the bottom of the spout area, and the degree of gas pulsation is the highest at the junction of the annulus area and spout area. The temperatures of gas, liquid, and particles are also analyzed. The results demonstrate that in the spout area, the gas temperature is much higher than that of the liquid and particles, but the three phases are uniformly mixed and have similar temperatures in other areas. Moreover, water vaporization mainly occurs at the junction of the annulus area and the spout area, a small amount of liquid is vaporized at the center of the spout area, and basically no vaporization reaction occurs in the outer radius of the annulus area. With the increase in gas velocity, gas temperature, and liquid temperature and the decrease in gas humidity, water vaporization reaction is promoted.
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http://dx.doi.org/10.1021/acsomega.0c05056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7860078PMC
February 2021

Trauma, Sleep and Mental Health Problems in Low-Income Young Adults.

Int J Environ Res Public Health 2021 01 28;18(3). Epub 2021 Jan 28.

School of Social Work, University of Pittsburgh, Pittsburgh, PA 15260, USA.

Low-income young adults are more likely to have exposure to trauma, which increases risk for mental health problems. Although adequate sleep promotes good health, people with histories of trauma are more likely to have sleep problems. The current study explored whether poor sleep mediated the relationship between trauma exposure and mental health. A sample of 143 low-income 18-24-year-old young adults completed depression, anxiety, and trauma exposure measures and wore sleep monitors for four nights. Structural equation modeling (SEM) was used to examine both direct and indirect effects of variables. Results showed that higher trauma exposure was associated with depression and anxiety. Mean sleep hours per night was fewer than six, far below recommended guidelines for optimal health and functioning. Fewer sleep hours partially mediated the relationship between both trauma exposure and depression and anxiety, and the direct effect from trauma remained significant after adjusting for the partial mediation from sleep.
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http://dx.doi.org/10.3390/ijerph18031145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908203PMC
January 2021

Anti-cerebral ischemia reperfusion injury of polysaccharides: A review of the mechanisms.

Biomed Pharmacother 2021 May 29;137:111303. Epub 2021 Jan 29.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, PR China.

Cerebral ischemia-reperfusion injury can lead to a series of serious brain diseases and cause death or different degrees of disability. Polysaccharide is a kind of biological macromolecule with multiple pharmacological activities and has been proven that it may be used for the treatment of cerebral I/R injury in the future. By sorting out all relevant research from 2000 to 2020, we selected 74 references and identified 22 kinds of polysaccharides. Almost all of these polysaccharides are extracted from traditional Chinese medicine. Research shows that these polysaccharides can improve cerebral ischemia-reperfusion injury through anti-oxidative stress, inhibiting the neuroinflammation, glutamate neurotoxicity and neuronal apoptosis, and exerting neurotrophic effect. The specific mechanisms include clearing ROS and RNS, inhibiting the expression of inflammatory factors, maintaining mitochondrial homeostasis and blocking caspase cascade, regulating NMDA receptor and promoting angiogenesis. We hoped this review is instructive for researchers to design, research and develop polysaccharides.
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http://dx.doi.org/10.1016/j.biopha.2021.111303DOI Listing
May 2021

A Systematic Review and Meta-Analysis of Health Utility Estimates in Chronic Spontaneous Urticaria.

Front Med (Lausanne) 2020 9;7:543290. Epub 2020 Dec 9.

Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China.

Chronic spontaneous urticaria (CSU) is a common recurrent skin disease that adversely affect patient's quality of life condition to treat. Economic evaluations of health care often include patient preferences for health outcomes using utilities. The study aimed to determine pooled estimates of utility-based quality of life in patients with CSU. We conducted a systematic review, meta-analysis, and meta-regression of peer-reviewed articles and conference papers that published from database inception to 31 April 2019 that reported utility estimates in patients with CSU. Scores reported with the EQ-5D, SF-6D, SF-12, and SF-36 instruments were converted to utilities using published mapping algorithms. Meta-analysis was used to calculate the pooled and meta-regression was used to examine the effects of possible factors. The pooled utility estimate for CSU was 0.68 [95% confidence interval (CI): 0.67-0.70]. The pooled utility estimate that converted from SF-36 or SF-12 was 0.66 (95% CI: 0.58-0.74), 0.72 (95% CI: 0.70-0.74) for EQ-5D, and 0.65 (95% CI: 0.63-0.67) for SF-6D, respectively. According to the meta-regression, higher proportion of female patients was significantly associated lower utility estimates ( = 0.013). The study provides evidence-based utility estimates to inform health-related burden analysis of CSU and reference for the follow-up cost-effectiveness evaluation of chronic spontaneous urticaria intervention. These results highlight differences in common utility-based instruments and need to be cognizant of the specific instruments used when comparing the results of outcome studies.
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http://dx.doi.org/10.3389/fmed.2020.543290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793814PMC
December 2020

Puerarin prevents cadmium-induced disorder of testicular lactic acid metabolism in rats by activating 5' AMP-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) signaling pathway.

Environ Toxicol 2021 May 6;36(5):945-957. Epub 2021 Jan 6.

College of Veterinary Medicine, Yangzhou University, Yangzhou, China.

Cadmium (Cd) interferes with the function of the male reproductive system; however, the molecular mechanism is poorly understood. This study aimed to evaluate the effect of puerarin (PU) on Cd-induced testicular lactic acid metabolism disorder. Weaning male Sprague-Dawley rats were pre-fed for 7 days, weighed, and randomly divided into four groups: Control group, CdAc group, CdAc  + PU group, PU group. The results showed that Cd accumulated in the testis, the testicles became congested and shrank, and the testis index decreased in the rats treated in the CdAc group. Cadmium exposure reduced the serum concentration of testosterone, and the concentration of lactic acid and pyruvate in the testis. Cd decreased testicular superoxide dismutase activity and total antioxidant capacity, and increased testicular malondialdehyde levels. Cd reduced the level of ATP, glycolytic gene expression, and lactate production-related proteins in the testis. Cd also decreased the expression of 5' AMP-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) signaling pathway-related proteins in the testis. However, these negative effects were attenuated by PU administration. In summary, Cd reduces the production of lactic acid in the testis of rats, while PU administration restores the production of lactic acid and reduces the toxicity of Cd to the testis of rats.
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http://dx.doi.org/10.1002/tox.23096DOI Listing
May 2021

Convenient CNT-Paper Gas Sensors Prepared by a Household Inkjet Printer.

ACS Omega 2020 Dec 17;5(51):32877-32882. Epub 2020 Dec 17.

School of Chemistry, Biology and Materials Engineering, Suzhou University of Science and Technology, Suzhou, Jiangsu 215009, P. R. China.

A hydrosoluble light-sensitive polymer named PSAG (poly-styrenesulfonate acrylic acid glycidyl methacrylate) was synthesized by acrylic acid (AA), sodium 4-styrenesulfonate (SS), and glycidyl methacrylate (GMA). PSAG is used to modify multiwall carbon nanotubes (MWCNTs) with a length diameter between 0.004 and 0.016. An inkjet conductive ink was formed by well-dispersed MWCNTs in aqueous and organic solvents, which could adjust the surface tension and viscosity of the ink. Gas sensors were then fabricated using this conductive ink on a household inkjet printer. The sensors demonstrated good reproducibility and acceptable recovery time (<200 s) to ammonia, methanol, and acetone. The resistance of the inkjet-printed sensor electrodes remained stable in the process of bending the sensors to different angles because of ultraviolet curing.
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http://dx.doi.org/10.1021/acsomega.0c02869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774069PMC
December 2020

Gap Junction Intercellular Communication Negatively Regulates Cadmium-Induced Autophagy and Inhibition of Autophagic Flux in Buffalo Rat Liver 3A Cells.

Front Pharmacol 2020 26;11:596046. Epub 2020 Nov 26.

College of Veterinary Medicine, Yangzhou University, Yangzhou, China.

Cadmium is an important environmental pollutant that poses a serious threat to the health of humans and animals. A large number of studies have shown that the liver is one of the important target organs of cadmium. Stimulation of cells can lead to rapid changes in gap junction intercellular communication (GJIC) and autophagy. Previous studies have shown that cadmium can inhibit GJIC and induce autophagy. In order to understand the dynamic changes of GJIC and autophagy in the process of cadmium-induced hepatotoxic injury and the effects of GJIC on autophagy, a time-gradient model of cadmium cytotoxicity was established. The results showed that within 24 h of cadmium exposure, 5 μmol/L cadmium inhibited GJIC by down regulating the expression levels of connexin 43 (Cx43) and disturbing the localization of Cx43 in Buffalo rat liver 3A (BRL 3A) cells. In addition, cadmium induced autophagy and then inhibited autophagic flux in the later stage. During this process, inhibiting of GJIC could exacerbate the cytotoxic damage of cadmium and induce autophagy, but further blocked autophagic flux, promoting GJIC in order to obtain the opposite results.
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http://dx.doi.org/10.3389/fphar.2020.596046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774522PMC
November 2020

The Importance of Cancer Registry Linkage for Studying Rare Cancers in Prospective Cohorts.

J Cancer Epidemiol 2020 25;2020:2895276. Epub 2020 Nov 25.

School of Public Health, University of Alberta, 3-300 ECHA 11405 - 87 Ave, Edmonton, Canada T6G 1C9.

Large prospective cohort studies may offer an opportunity to study the etiology and natural history of rare cancers. Cancer diagnoses in observational cohort studies are often self-reported. Little information exists on the validity of self-reported cancer diagnosis, especially rare cancers, in Canada. This study evaluated the validity of self-reported cancer diagnosis in Alberta's Tomorrow Project (ATP), a provincial cohort in Canada. ATP data were linked to the Alberta Cancer Registry (ACR). The first instance of self-reported cancer in a follow-up survey was compared to the first cancer diagnosis in the ACR after enrollment. The sensitivity and positive predictive value (PPV) were estimated for the reporting of cancer status, reporting of common or rare cancer, and reporting of site-specific cancer. Logistic regression analysis explored factors associated with false positive, false negative, and incorrect cancer site reporting. In the 30,843 ATP participants who consented to registry linkage, there were 810 primary cancer diagnoses in the ACR and 959 self-reports of first cancer post-enrollment, for a cancer status sensitivity of 92.1% (95% CI: 90.0-93.9) and PPV of 77.8% (95% CI: 75.0-80.4). Compared to common cancers, rare cancers had a lower sensitivity (62.8% vs. 89.6%) and PPV (35.8% vs. 84.5%). Participants with a rare cancer were more likely to report an incorrect site than those with a common cancer. Rare cancers were less likely to be captured by active follow-up than common cancers. While rare cancer research may be feasible in large cohort studies, registry linkage is necessary to capture rare cancer diagnoses completely and accurately.
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http://dx.doi.org/10.1155/2020/2895276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718062PMC
November 2020

Comparison of Combined Lumbosacral Plexus and T12 Paravertebral Nerve Blocks With General Anesthesia in Older Adults Undergoing Primary Total Hip Arthroplasty: A Retrospective, Propensity Score-Matched Cohort Study.

Geriatr Orthop Surg Rehabil 2020 24;11:2151459320976531. Epub 2020 Nov 24.

Hospital Medical Records Statistics Office, The Changzhou First People's Hospital, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, People's Republic of China.

Background: Total Hip Arthroplasty(THA) is a surgical treatment for hip disease. A large amount of evidence has been reported on comparing outcomes of neuraxial(spinal or epidural) anesthesia and general anesthesia. However, it is unclear whether nerve blocks(NB) as main anesthesia technique compared with general anesthesia(GA) for THA could reduce perioperative complications. We conducted a retrospective evaluation of NB and GA, using a propensity score-matched analysis(PSMA).

Methods: A total of 902 patients older than 60 years old with hip disease undergoing primary THA received combined lumbosacral plexus and T paravertebral nerve blocks (n = 143) or GA (n = 759) at our institution from 2012 to 2018.Binary logistic regression was used for comparison of the primary outcomes(the incidence of delirium) and the secondary outcomes(the percentage of postoperative hemoglobin(>10g/dl), transfusion(>2 units), major cardiac events(MACE), postoperative pulmonary complications (PPC) and 30-day mortality) in the matched cohorts.Statistical analysis was performed using SPSS v 23.0.

Results: Both cohorts were balanced in all included parameters after PSMA. The incidence of delirium was lower (OR 0.233,95% CI 0.064-0.845, p = 0.030) in NB group in matched cohorts. In the unmatched and matched cohorts, the percentage of Hb(>10g/dl) was higher in GA group but the incidence of transfusion(>2 units) was higher in NB group. No difference was found in the incidence of 30-day mortality in the unmatched and matched cohorts. In the unmatched cohorts, the incidence of PPC was higher and LOS was longer in NB cohort, but no difference was observed in the matched cohorts.

Conclusion: In patients older than 60 years old receiving primary THA, NB could be associated with a lower incidence of delirium.
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http://dx.doi.org/10.1177/2151459320976531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691895PMC
November 2020