Publications by authors named "Yan Xiong"

731 Publications

Ultrasensitive and highly stretchable fibers with dual conductive microstructural sheaths for human motion and micro vibration sensing.

Nanoscale 2022 Jan 21. Epub 2022 Jan 21.

School of Optical Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Conductive and stretchable fibers are important components of the increasingly popular wearable electronic devices as they meet the design requirements of excellent electrical conductivity, stretchability, and wearability. In this work, we developed a novel dual conductive-sheath fiber (DCSF) with a conductive sheath composed of a porous elastic conductive layer and cracked metal networks, thus achieving ultrahigh sensitivity under a large strain range. The core of the DCSF is made of thermoplastic polyurethane (TPU) elastic fiber wrapped in a porous stretchable conductive layer composed of carbon nanotubes (CNTs) and TPU. Next, a layer of gold film is deposited on the surface of the porous stretchable conductive layer by ion beam sputtering. Due to the fast response time of 184 ms and ultrahigh sensitivity in the 0-100% strain range (a gauge factor of 184.50 for a strain of 0-10%, 4.12 × 10 for 10%-30%, and 2.80 × 10 for 30%-100%) of the DCSF strain sensor, we successfully wove the fiber strain sensor into gloves and could realize the recognition of different hand gestures. Also the DCSF strain sensor can be applied to detect microvibrations efficiently. The demonstrated DCSF has potential applications in the development of smart wearable devices and micro vibration sensors.
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http://dx.doi.org/10.1039/d1nr08380eDOI Listing
January 2022

Comparison of zero echo time MRI with T1-weighted fast spin echo for the recognition of sacroiliac joint structural lesions using CT as the reference standard.

Eur Radiol 2022 Jan 21. Epub 2022 Jan 21.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.

Objectives: To evaluate the diagnostic performance of zero echo time (ZTE) MRI in the depiction of structural lesions of sacroiliac joints (SIJs) in patients with the suspicion of sacroiliitis compared with T1-weighted fast spin echo (T1 FSE), using CT as the reference standard.

Methods: Forty patients with suspicion of sacroiliitis underwent both CT and MR scans of SIJs with 80 SIJs (160 bone articular surfaces) included for analysis. Two readers independently scored SIJs for structural lesions on CT and MR images. The diagnostic capability of ZTE MRI and T1 FSE were compared by the McNemar test, using CT as the reference standard. Agreements of diagnosis and sum scores of lesions between MR sequences and CT as well as between readers were also investigated using Cohen's κappa tests and intraclass correlation coefficients.

Results: Diagnostic accuracy of ZTE MRI was higher than that of T1 FSE for erosions, sclerosis, and joint space changes (e.g., joint space changes: 91.3% vs 75.0%). ZTE MRI also improved sensitivity for detection of erosions and sclerosis (e.g., erosions at the joint level: 98.2% vs 80.0%) as well as specificity for detection of joint space changes (93.0% vs 67.4%). ZTE MRI had more consistency with CT than T1 FSE for both diagnosis and sum scores. Inter-reader agreements were higher for CT and ZTE MRI than those for T1 FSE.

Conclusions: ZTE MRI showed superior diagnostic performance in the depiction of SIJ structural lesions compared with routine T1-weighted MRI and had reliability comparable to CT.

Key Points: • ZTE MRI can provide CT-like bone contrast for the depiction of osseous structural lesions of the sacroiliac joints. • ZTE MRI showed superior diagnostic performance than conventional T1 FSE in the detection of osseous structural lesions of sacroiliitis, using CT as the reference standard. • In terms of inter-reader reliability, ZTE MRI performed comparably to CT and better than conventional T1 FSE.
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http://dx.doi.org/10.1007/s00330-021-08513-5DOI Listing
January 2022

Interaction between arteriosclerosis and the APOE4 gene in cognitive decline in older adults: a cross-sectional study in rural minority areas in western China.

Psychogeriatrics 2022 Jan 19. Epub 2022 Jan 19.

School of Public Health, Guizhou Medical University, Guiyang, China.

Background: Numerous studies have confirmed that the apolipoprotein E4 allele (APOE4) gene and arteriosclerosis (AS) have a combined effect on the occurrence of cognitive function impairment, and dyslipidaemia levels are significantly correlated with APOE4 levels and AS. Few studies have focused on the combined effect of the APOE4 gene and AS on cognitive function. Therefore, this study aimed to investigate the effect of APOE4 gene and AS acting together on cognitive function through dyslipidaemia levels, which could provide certain scientific research value for future studies.

Methods: A multi-stage cluster sampling method was used to investigate older adults aged 60 years and above in rural areas of Guizhou, China. The demographic sociological characteristics were collected, and laboratory tests, blood lipid measurements, and physical examinations were performed. Mini-Mental State Examination (MMSE) was used to determine cognitive function. Analysis of variance with two-factor factorial design was used to analyse the interaction between the APOE4 gene and AS on cognitive function and its domains.

Results: A total of 549 elderly subjects were eligible for this study. The result of the factorial design analysis revealed there was a significant interaction between the APOE4 gene and AS in terms of attention and numeracy (F = 6.878, P = 0.009).

Conclusions: The combination of the APOE4 gene and AS leads to a decrease in the level of attention and numeracy domains, and certain attention should be focused on such populations in the future.
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http://dx.doi.org/10.1111/psyg.12808DOI Listing
January 2022

Prevalence and clinical significance of serum sodium variability in patients with acute-on-chronic liver diseases: a prospective multicenter study in China.

Hepatol Int 2022 Jan 17. Epub 2022 Jan 17.

Hepatology Unit, Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: No reports exist regarding the prevalence of different Na levels and their relationship with 90-day prognosis in hospitalized patients with acute-on-chronic liver disease (AoCLD) in China. Therefore, the benefit of hyponatremia correction in AoCLD patients remains unclear.

Methods: We prospectively collected the data of 3970 patients with AoCLD from the CATCH-LIFE cohort in China. The prevalence of different Na levels (≤ 120; 120-135; 135-145; > 145) and their relationship with 90-day prognosis were analyzed. For hyponatremic patients, we measured Na levels on days 4 and 7 and compared their characteristics, based on whether hyponatremia was corrected.

Results: A total of 3880 patients were involved; 712 of those developed adverse outcomes within 90 days. There were 80 (2.06%) hypernatremic, 28 (0.72%) severe hyponatremic, and 813 (20.95%) mild hyponatremic patients at admission. After adjusting for all confounding factors, the risk of 90-day adverse outcomes decreased by 5% (odds ratio [OR] 0.95; 95% confidence interval [CI] 0.93-0.97; p < 0.001), 24% (OR 0.76; 95% CI 0.70-0.84; p < 0.001), and 42% (OR 0.58; 95% CI 0.49-0.70; p < 0.001) as Na level increased by 1, 5, and 10 mmol/L, respectively. Noncorrection of hyponatremia on days 4 and 7 was associated with 2.05-fold (hazard ratio [HR], 2.05; 95% CI, 1.50-2.79; p < 0.001) and 1.46-fold (HR 1.46; 95% CI 1.05-2.02; p = 0.028) higher risk of adverse outcomes.

Conclusions: Hyponatremia was an independent risk factor for a poor 90-day prognosis in patients with AoCLD. Failure to correct hyponatremia in a week after admission was often associated with increased mortality. (ClinicalTrials.gov number: NCT02457637, NCT03641872).

Clinical Trial Numbers: This study is registered at Shanghai www.clinicaltrials.org (NCT02457637 and NCT03641872).
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http://dx.doi.org/10.1007/s12072-021-10282-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761510PMC
January 2022

A renewable electrochemical sensor based on a self-assembled framework of chiral molecules for efficient identification of tryptophan isomers.

Anal Chim Acta 2022 Jan 11;1191:339276. Epub 2021 Nov 11.

Chongqing Research Center for Pharmaceutical Engineering, School of Pharmacy, Chongqing Medical University, Chongqing, 400016, China. Electronic address:

Molecular self-assembly provides a reasonably effective strategy for the design and construction of chiral sensors. Here, Cu was connected to β-cyclodextrin (β-CD) through coordination to synthesize Cu-β-CD, subsequently assembled with ammoniated chitosan-MWCNTs (NH-CS-MWCNTs) by the effect of coordination driver to form a chiral sensing interface Cu-β-CD/NH-CS-MWCNTs. Using the electrochemical method, the valid recognition of tryptophan (Trp) isomers was achieved on the self-assembly interface. Under the optimal experimental conditions, the developed sensor exhibited good linearity and satisfactorily renewable ability. Cu-β-CD/NH-CS-MWCNTs/GCE showed the capacity to predict the ratio of D-Trp and L-Trp in racemic mixtures and the possibility of qualitative and quantitative determination for Trp isomers. Finally, the electrochemical sensor was used to detect the Trp enantiomers in rat serum, further verifying the feasibility of the sensor in the determination of actual samples. Therefore, the electrochemical chiral sensor not only is used for the recognition of Trp enantiomers but shows great potential in practical applications.
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http://dx.doi.org/10.1016/j.aca.2021.339276DOI Listing
January 2022

Evaluation of the degenerative lumbar osseous morphology using zero echo time magnetic resonance imaging (ZTE-MRI).

Eur Spine J 2022 Jan 11. Epub 2022 Jan 11.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095, Jiefang Road, Wuhan, 430030, Hubei Province, China.

Purpose: To determine and compare the performance of zero echo imaging (ZTE) with conventional MRI sequences on lumbar osseous morphology in patients suspected with lumbar degeneration with multi-slice computed tomography (MSCT) as standard reference.

Methods: 22 subjects with concerned lumbar degeneration were recruited. All subjects were scanned with ZTE sequence after routine conventional MR sequences on a 3.0 T system and also received MSCT examination. Image quality was assessed. The quantitative and qualitative parameters of lumbar osseous morphology on MSCT, ZTE and MRI images were evaluated by three musculoskeletal radiologists independently. Inter-reader and inter-modality reliability and the difference between the modalities were calculated.

Results: There was no difference for the osseous parameters between modalities, including axial orientation (p = 0.444), IAD (p = 0.381), lateral recess (p = 0.370), pedicle width (p = 0.067), pedicle height (p = 0.056), and osteophyte grade (p = 0.052). The measurement of the foramina diameter was statistically different between conventional MRI and MSCT (p < 0.05) but not between the MSCT and ZTE (p = 0.660). Conventional MRI was more likely to miss cortical bone abnormalities. ZTE appeared blurrier in cortical bone than MSCT, especially in cases with severe lumbar degeneration. The inter-reader agreement between MSCT and ZTE-MRI was higher than between MSCT and conventional MRI.

Conclusions: ZTE-MRI could offer more cortical bone details than conventional MRI images and might be a valid alternative to CT for lumbar osseous morphology assessment to some extent.
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http://dx.doi.org/10.1007/s00586-021-07099-2DOI Listing
January 2022

Convergent alteration of lung tissue microbiota and tumor cells in lung cancer.

iScience 2022 Jan 16;25(1):103638. Epub 2021 Dec 16.

Department of Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiao Tong University, No. 241 West Huaihai Road, Shanghai 200030, China.

Microbiota-host interaction plays an important role in cancer predisposing, initiation, progression, and response to therapy. Here, we explored the composition of lung tissue microbiota in 143 Chinese patients through conducting 16S rRNA gene sequencing, while TP53 mutation in tumor cells was assessed simultaneously. We found PAH-degrading microbes were more abundant in lung tumor microbiota from smokers. Furthermore, TP53 mutation was more prevalent in smokers, and TP53-mutated tumor harbored more , as well as that was also capable of degrading PAH. Further analysis showed DNA recombination and repair pathway was enriched in microbiota of smokers, which was convergent to the alteration occurred in tumor cells. Meanwhile, the microbiota of TP53-mutated tumor also exhibited dysregulation of p53 signaling pathway. Our results provided insights into the association of lung commensal microbes with tobacco exposure and host gene mutation, suggesting microbiota and tumor cells might undergo convergent alteration and mutually benefit each other.
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http://dx.doi.org/10.1016/j.isci.2021.103638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718896PMC
January 2022

SARS-CoV-2 spillover into hospital outdoor environments.

J Hazard Mater Lett 2021 Nov 19;2:100027. Epub 2021 May 19.

School of Environment, Tsinghua University, Beijing, 100084, PR China.

Facing the ongoing coronavirus infectious disease-2019 (COVID-19) pandemic, many studies focus on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in indoor environment, on solid surface or in wastewater. It remains unclear whether SARS-CoV-2 can spill over into outdoor environments and impose transmission risks to surrounding people and communities. In this study, we investigated the presence of SARS-CoV-2 by measuring viral RNA in 118 samples from outdoor environment of three hospitals in Wuhan. We detected SARS-CoV-2 in soils (205-550 copies/g), aerosols (285-1,130 copies/m) and wastewaters (255-18,744 copies/L) in locations close to hospital departments receiving COVID-19 patients or in wastewater treatment sectors. These findings revealed a significant viral spillover in hospital outdoor environments that was possibly caused by respiratory droplets from patients or aerosolized particles from wastewater containing SARS-CoV-2. In contrast, SARS-CoV-2 was not detected in other areas or on surfaces with regular implemented disinfection. Soils may behave as viral warehouse through deposition and serve as a secondary source spreading SARS-CoV-2 for a prolonged time. For the first time, our findings demonstrate that there are high-risk areas out of expectation in hospital outdoor environments to spread SARS-CoV-2, calling for sealing of wastewater treatment unit and complete sanitation to prevent COVID-19 transmission risks.
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http://dx.doi.org/10.1016/j.hazl.2021.100027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132549PMC
November 2021

Plant TOR signaling network: Complexes, conservations and specificities.

J Integr Plant Biol 2021 Dec 28. Epub 2021 Dec 28.

College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Target of Rapamycin (TOR) is an evolutionarily conserved protein kinase that functions as a central signaling hub to integrate diverse internal and external cues to precisely orchestrate cellular and organismal physiology. During evolution, TOR both maintains the highly conserved TOR complex compositions, cellular and molecular functions, but also evolves distinctive roles and strategies to modulate cell growth, proliferation, metabolism, survival and stress responses in eukaryotes. Here, we review recent discoveries on the plant TOR signaling network. We present an overview of plant TOR complexes, analyze the signaling landscape of the plant TOR signaling network from the upstream signals that regulate plant TOR activation to the downstream effectors involved in various biological processes, and compare their conservation and specificities within different biological contexts. Finally, we summarize the impact of dysregulation of TOR signaling on every stage of plant growth and development, from embryogenesis and seedling growth, to flowering and senescence. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/jipb.13212DOI Listing
December 2021

Exploring the mechanism of Buxue Yimu Pill on hemorrhagic anemia through molecular docking, network pharmacology and experimental validation.

Chin J Nat Med 2021 Dec;19(12):900-911

Department of Chemistry, School of Chemistry, Xiangtan University, Xiangtan 411105, China.

Buxue Yimu Pill (BYP) is a classic gynecological medicine in China, which is composed of Angelica sinensis (Oliv.) Diels, Leonurus japonicus Houtt, Astragalus membranaceus (Fisch.) Bunge, Colla corii asini and Citrus reticulata Blanco. It has been widely used in clinical therapy with the function of enriching Blood, nourishing Qi, and removing blood stasis. The current study was designed to determine the bioactive molecules and therapeutic mechanism of BYP against hemorrhagic anemia. Herein, GC-MS and UPLC/Q-TOF-MS/MS were employed to identify the chemical compounds from BYP. The genecards database (https: //www.genecards.org/) was used to obtain the potential target proteins related to hemorrhagic anemia. Autodock/Vina was adopted to evaluate the binding ability of protein receptors and chemical ligands. Gene ontology and KEGG pathway enrichment analysis were conducted using the ClusterProfiler. As a result, a total of 62 candidate molecules were identified and 152 targets related to hemorrhagic anemia were obtained. Furthermore, 34 active molecules and 140 targets were obtained through the virtual screening experiment. The data of molecular-target (M-T), target-pathway (T-P), and molecular-target-pathway (M-T-P) network suggested that 32 active molecules enhanced hematopoiesis and activated the immune system by regulating 57 important targets. Pharmacological experiments showed that BYP significantly increased the counts of RBC, HGB, and HCT, and significantly down-regulated the expression of EPO, IL-6, CSF3, NOS2, VEGFA, PDGFRB, and TGFB1. The results also showed that leonurine, leonuriside B, leosibiricin, ononin, rutin, astragaloside I, riligustilide and levistolide A, were the active molecules closely related to enriching Blood. In conclusion, based on molecular docking, network pharmacology and validation experiment results, the enriching blood effect of BYP on hemorrhagic anemia may be associated with hematopoiesis, anti-inflammation, and immunity enhancement.
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http://dx.doi.org/10.1016/S1875-5364(21)60104-8DOI Listing
December 2021

Deep mining and quantification of oxidized cholesteryl esters discovers potential biomarkers involved in breast cancer by liquid chromatography-mass spectrometry.

J Chromatogr A 2022 Jan 20;1663:462764. Epub 2021 Dec 20.

College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong 266003, China. Electronic address:

Oxidized cholesteryl ester (OxCE) is produced by the oxidation of cholesteryl ester (CE) in the cores of lipoproteins. OxCE production and oxidative stress have been largely associated with breast cancer. Herein, we developed a novel reverse-phase liquid chromatography coupling quadrupole time-of-flight mass spectrometry (RPLC‒Q-TOF‒MS) method based on the iterative acquisition mode and used the MS/MS mode for deep mining and simultaneous quantification of cholesterol (Chol), CEs and OxCEs in human serum. A mathematical model was used to globally profile 57 molecular species of both CEs and OxCEs in the serum of both healthy volunteers and patients with breast cancer, and the qualitative results were verified based on the retention regularity. An abnormal elevation of OxCEs was found in serum samples of breast cancer patients, where OxCEs were produced by the oxidation of the fatty acyl chain of CE (20:4), such as CE (20:1)+3O, CE (20:2)+2O and CE (20:3)+O, which could be regarded as biomarkers. This comprehensive method for the global profiling of Chol, OxCEs and CEs sheds light on the role OxCEs and CEs play in breast cancer and has enabled the discovery of breast cancer biomarkers.
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http://dx.doi.org/10.1016/j.chroma.2021.462764DOI Listing
January 2022

Quasi-Phthalocyanine Conjugated Covalent Organic Frameworks with Nitrogen-Coordinated Transition Metal Centers for High-Efficiency Electrocatalytic Ammonia Synthesis.

Nano Lett 2022 Jan 22;22(1):372-379. Epub 2021 Dec 22.

MOE Key Laboratory of Mesoscopic Chemistry, MOE Key Laboratory of High Performance Polymer Materials and Technology, Jiangsu Key Laboratory of Advanced Organic Materials, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210023, China.

Developing high-performance nitrogen reduction reaction (NRR) electrocatalysts is an ongoing challenge. Herein, we report a pyrolysis-free synthetic method for introducing ordered quasi-phthalocyanine N-coordinated transition metal (Ti, Cu, or Co) centers into a conjugated two-dimensional (2D) covalent organic framework (COF) for enhanced NRR performance. Detailed experiments and characterizations revealed that the NRR activity of Ti-COF was clearly better than that of Cu-COF and Co-COF, because of the superior abilities of Ti metal centers in activating inert N molecules and suppressing the hydrogen evolution reaction (HER). The resulting Ti-COF exhibits a high NH yield of 26.89 μg h mg and a Faradaic efficiency of 34.62% for NRR. Density functional theory (DFT) calculations verify that Ti-COF can effectively adsorb and activate N molecules and inhibit HER compared with Cu-COF, Co-COF, and pristine COF catalysts. This work opens a new avenue for developing 2D-COF materials that contain abundant coordinated transition metal centers toward electrocatalytic NRR.
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http://dx.doi.org/10.1021/acs.nanolett.1c04009DOI Listing
January 2022

Small-diameter p-type SnS nanowire photodetectors and phototransistors with low-noise and high-performance.

Nanotechnology 2022 Jan 7;33(13). Epub 2022 Jan 7.

School of Physics and Optoelectronic Engineering, Yangtze University, Jingzhou 434023, People's Republic of China.

P-type nanostructured photodetectors and phototransistors have been widely used in the field of photodetection due to their excellent electrical and optoelectronic characteristics. However, the large dark current of p-type photodetectors will limit the detectivity. Herein, we synthesized small-diameter single-crystalline p-type SnS nanowires (NWs) and then fabricated single SnS NW photodetectors and phototransistors. The device exhibits low noise and low dark current, and its noise current power is as low as 2.4 × 10A. Under 830 nm illumination and low power density of 0.12 mW cm, the photoconductive gain, responsivity and detectivity of the photodetector are as high as 3.9 × 10, 2.6 × 10A Wand 1.8 × 10Jones, respectively, at zero gate voltage. The rise and fall time of response are about 9.6 and 14 ms. The experimental results show that the small-diameter p-type SnS NWs have broad application prospects in high-performance and low-power photodetectors with high sensitivity, fast response speed and wide spectrum detection in the future.
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http://dx.doi.org/10.1088/1361-6528/ac451fDOI Listing
January 2022

Multimodal Diagnostic Workup of Primary Pericardial Mesothelioma: A Case Report.

Front Cardiovasc Med 2021 19;8:758988. Epub 2021 Nov 19.

Department of Cardiology, Institute of Cardiovascular Disease, Peking University First Hospital, Beijing, China.

Primary pericardial mesothelioma is an extremely rare tumor, and early identification and accurate diagnosis may improve its clinical outcome. In this study, we reported a case of a 70-year-old woman who presented with dyspnea. Conventional transthoracic echocardiography showed massive pericardial effusion. Contrast-enhanced ultrasonography revealed a hyper-enhancing mass in the pericardium. Further imaging methods, including cardiac MRI and positron emission tomography/computed tomography, showed invasion of the pericardial mass into the adjacent tissues and distant metastases. Pathologic examination of a puncture biopsy specimen finally confirmed the diagnosis of PPM. Pericardial masses are difficult to detect when a large amount of pericardial effusion is present and the mass is small. The combination of multiple modalities plays a meaningful role in identifying PPM.
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http://dx.doi.org/10.3389/fcvm.2021.758988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639695PMC
November 2021

Increased INR Values Predict Accelerating Deterioration and High Short-Term Mortality Among Patients Hospitalized With Cirrhosis or Advanced Fibrosis.

Front Med (Lausanne) 2021 18;8:762291. Epub 2021 Nov 18.

Chinese Chronic Liver Failure Consortium, China.

An increase in the international normalized ratio (INR) is associated with increased mortality in patients with cirrhosis and other chronic liver diseases, while little is known about the quantitative relationship. This study aimed to investigate the quantitative relationship between the INR and short-term prognosis among patients hospitalized with cirrhosis or advanced fibrosis and to evaluate the role of the INR as a risk factor for short-term liver transplant (LT)-free mortality in these patients. This study prospectively analyzed multicenter cohorts established by the Chinese Acute-on-Chronic Liver Failure (CATCH-LIFE) study. Cox regression was used to describe the relationship between the INR and independent risk factors for short-term LT-free mortality. Forest plots were used in the subgroup analysis. Generalized additive models (GAMs) and splines were used to illustrate the quantitative curve relationship between the INR and the outcome and inflection point on the curve. A total of 2,567 patients with cirrhosis and 924 patients with advanced fibrosis were included in the study. The 90-day LT-free mortality of patients with cirrhosis and advanced fibrosis was 16.7% (428/2,567) and 7.5% (69/924), respectively. In the multivariable Cox regression analysis, the increase in the INR was independently associated with the risk of 90-day LT-free mortality both in patients with cirrhosis (HR, 1.06; 95% CI, 1.04-1.07, < 0.001) and in patients with advanced fibrosis (HR, 1.09; 95% CI, 1.06-1.12, < 0.001). An INR of 1.6/1.7 was found to be the starting point of coagulation dysfunction with a rapid increase in mortality in patients with cirrhosis or in patients with advanced fibrosis, respectively. A 28-day LT-free mortality of 15% was associated with an INR value of 2.1 in both cirrhosis and advanced fibrosis patients. This study was the first to quantitatively describe the relationship between the INR and short-term LT-free mortality in patients with cirrhosis or advanced fibrosis. The starting points of INR indicating the rapid increase in mortality and the unified cutoff value of coagulation failure in cirrhosis and advanced fibrosis, will help clinicians accurately recognize early disease deterioration.
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http://dx.doi.org/10.3389/fmed.2021.762291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637055PMC
November 2021

[Prospective Study on Corneal Safety Evaluation of Children/Adolescents with Low and Moderate Myopia after Long-Term Orthokeratology].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 Nov;52(6):1006-1010

Department of Optometry and Visual Science, West China School of Medicine, Sichuan University, Chengdu 610041, China.

Objective: To observe the possible changes in the integrity of the cornea and corneal endothelial cells of children/adolescents with low or moderate myopia after long-term wearing of orthokeratology (ortho-k) lenses, as well as the time when the relevant changes occur, so as to evaluate the safety of long-term wearing of ortho-k lens and to provide a reference for the safety evaluation of subjects wearing ortho-k lenses.

Methods: Subjects were recruited in the Contact Lens Clinic, West China Hospital, Sichuan University for a three-year prospective study. Ortho-k of the same brand was matched for the subjects. The central corneal thickness (CCT), corneal endothelial cell density (ECD), and hexagonal cell ratio (HEX) were measured prior to the wearing of ortho-k lenses and after wearing ortho-k lenses for 1 month, 3 months, 6 months, and every 3 months until 36 months. The results of corneal fluorescence staining were recorded during each follow-up. When corneal staining was observed, the Efron grading standard was used for grading and corresponding treatment was given.

Results: A total of 33 (66 eyes) myopic patients were included in the study. 15 cases (46.2%) reported having binocular foreign body sensations and tearing within the first week of wearing the lenses. After the subjects became adapted to wearing the lenses, the symptoms disappeared without intervention. During the follow-up period, 31 cases (93.94%) of binocular corneal staining were observed, of which, 24 cases (72.73%) were graded as G0, receiving no treatment, 5 cases (15.15%) were graded as GⅠ, and 2 cases (6.06%) were graded as GⅡ. Corresponding clinical treatment for corneal staining was given to the GⅠ and GⅡ subjects. This study found that the corneal ECD was inversely proportional to age ( =-0.380, =0.002). During the three-year follow-up period, the subjects' left eye ECD decreased from the baseline at 24 months and the right eye ECD decreased from the baseline at 27 months ( <0.05). The CCT results in the subjects showed that CCT became thinner at 1 month after wearing the lens ( <0.05), but the follow-up CCT showed a stable trend.

Conclusion: After three years of long-term follow-up, no serious corneal complications occurred in children/adolescents with moderate and low myopia after long-term wearing of ortho-k lens. The corneal ECD of both eyes started decreasing 24 months after wearing the ortho-k lenses and the CCT decreased 1 month after wearing the lenses.
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http://dx.doi.org/10.12182/20211160107DOI Listing
November 2021

Mechanical and Acoustic Properties of Jute Fiber-Reinforced Polypropylene Composites.

ACS Omega 2021 Nov 13;6(46):31154-31160. Epub 2021 Nov 13.

Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China.

Chemical fibers such as glass fiber and aramid cover a large proportion of sound-absorbing composite materials on the current commercial market. These materials possess superior mechanical properties but have the disadvantages of high production costs and energy consumption and difficult recovery and degradation. In this paper, jute fiber and polypropylene were selected as raw materials, and a series of jute fiber-reinforced polypropylene composite materials were prepared by a mixing-hot-pressing process. The acoustic and mechanical performances of the composites with different fiber contents and fiber residue ratios were discussed. The results showed that the sound absorption coefficient values increased with the increasing fiber content and decreasing residual gum ratio. The mechanical properties varied inversely with the residual gum rate. With the increase of fiber content, the tensile and bending strengths first increased and then decreased. Therefore, the jute fiber-reinforced polypropylene composites can possess favorable sound absorption performance with no mechanical property penalty by adjusting the parameters properly, demonstrating that the composite materials have promising applications in the acoustic field.
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http://dx.doi.org/10.1021/acsomega.1c04605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8613868PMC
November 2021

Self-Healing Hydrogel Embodied with Macrophage-Regulation and Responsive-Gene-Silencing Properties for Synergistic Prevention of Peritendinous Adhesion.

Adv Mater 2021 Nov 23:e2106564. Epub 2021 Nov 23.

Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Engineering Research Center for Orthopaedic Material Innovation and Tissue Regeneration, Shanghai, 200233, China.

Antiadhesion barriers such as films and hydrogels used to wrap repaired tendons are important for preventing the formation of adhesion tissue after tendon surgery. However, sliding of the tendon can compress the adjacent hydrogel barrier and cause it to rupture, which may then lead to unexpected inflammation. Here, a self-healing and deformable hyaluronic acid (HA) hydrogel is constructed as a peritendinous antiadhesion barrier. Matrix metalloproteinase-2 (MMP-2)-degradable gelatin-methacryloyl (GelMA) microspheres (MSs) encapsulated with Smad3-siRNA nanoparticles are entrapped within the HA hydrogel to inhibit fibroblast proliferation and prevent peritendinous adhesion. GelMA MSs are responsively degraded by upregulation of MMP-2, achieving on-demand release of siRNA nanoparticles. Silencing effect of Smad3-siRNA nanoparticles is around 75% toward targeted gene. Furthermore, the self-healing hydrogel shows relatively attenuated inflammation compared to non-healing hydrogel. The mean adhesion scores of composite barrier group are 1.67 ± 0.51 and 2.17 ± 0.75 by macroscopic and histological evaluation, respectively. The proposed self-healing hydrogel antiadhesion barrier with MMP-2-responsive drug release behavior is highly effective for decreasing inflammation and inhibiting tendon adhesion. Therefore, this research provides a new strategy for the development of safe and effective antiadhesion barriers.
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http://dx.doi.org/10.1002/adma.202106564DOI Listing
November 2021

Effect of Eriodictyol on Retinoblastoma via the PI3K/Akt Pathway.

J Healthc Eng 2021 11;2021:6091585. Epub 2021 Nov 11.

Department of Ophthalmology, Jingmen No. 1 People's Hospital, Xiangshan Road, Jingmen, Hubei, China.

Retinoblastoma (RB) is one of the most common intraocular malignancies in children, which causes vision loss and even threatens life. Eriodictyol is a natural flavonoid with strong anticancer activity. Some studies have shown that eriodictyol exerts anticancer effects in glioma, colon cancer, and lung cancer; however, no studies have reported the anticancer effects of eriodictyol on RB. Therefore, the aim of this study was to investigate the anticancer activity of eriodictyol against the RB Y79 cell line and its potential mechanism of action. Interestingly, we found that eriodictyol inhibited the proliferation, migration, and invasion of Y79 cells in a dose-dependent manner and decreased the expression of MMP-2 and MMP-9 proteins in the cells. In addition, eriodictyol-induced apoptosis in Y79 cells was assessed by flow cytometry and immunoblotting. Here, our study revealed that eriodictyol dose dependently inhibited the activation of the PI3K/Akt signaling pathway. Notably, the effect of eriodictyol on RB apoptosis was reversed by a PI3K agonist 740 Y-P. In conclusion, our study shows that eriodictyol effectively inhibits proliferation, migration, and invasion and induces apoptosis in RB cell lines, which may be the result of blocking the PI3K/Akt signaling pathway. Thus, eriodictyol may provide a new theoretical basis for exploring targeted antitumor natural therapies.
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http://dx.doi.org/10.1155/2021/6091585DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601792PMC
January 2022

CKD Stages, Bone Metabolism Markers, and Cortical Porosity Index: Associations and Mediation Effects Analysis.

Front Endocrinol (Lausanne) 2021 5;12:775066. Epub 2021 Nov 5.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Chronic kidney disease (CKD) has a significant negative impact on bone health. However, the mechanisms of cortical bone deterioration and cortical porosity enlargement caused by CKD have not been fully described. We therefore examined the association of CKD stages with cortical porosity index (PI), and explored potential mediators of this association. Double-echo ultrashort echo-time magnetic resonance imaging (UTE MRI) provides the possibility of quantifying cortical porosity . A total of 95 patients with CKD stages 2-5 underwent 3D double-echo UTE-Cones MRI (3.0T) of the midshaft tibia to obtain the PI. PI was defined as the ratio of the image signal intensity of a sufficiently long echo time (TE) to the shortest achievable TE. Parathyroid hormone (PTH), β-CrossLaps (β-CTX), total procollagen type I amino-terminal propeptide (T-P1NP), osteocalcin (OC), 25-hydroxyvitamin D (25OHD), and lumbar bone mineral density (BMD) were measured within one week of the MRI. Partial correlation analysis was performed to address associations between PI, eGFR and potential mediators (PTH, β-CTX, T-P1NP, OC, 25OHD, BMD, and T-score). Multiple linear regression models were used to assess the association between CKD stages and PI value. Then, a separate exploratory mediation analysis was carried out to explore the impact of CKD stages and mediators on the PI value. The increasing CKD stages were associated with a higher PI value (P < 0.001). The association of CKD stages and PI mediated 34.4% and 30.8% of the total effect by increased PTH and β-CTX, respectively. Our study provides a new idea to monitor bone health in patients with CKD, and reveals the internal mechanism of bone deterioration caused by CKD to some extent.
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http://dx.doi.org/10.3389/fendo.2021.775066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8602844PMC
November 2021

Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings.

STAR Protoc 2021 Dec 29;2(4):100922. Epub 2021 Oct 29.

Basic Forestry and Proteomics Research Center, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, P. R. China.

Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture system for efficient depletion of the endogenous carbon/nitrogen nutrients in Arabidopsis seedlings. Exogenous carbon/nitrogen can be supplied to dissect the TOR pathway. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2013) and Liu et al. (2021).
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http://dx.doi.org/10.1016/j.xpro.2021.100922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567432PMC
December 2021

Gq signaling in α cells is critical for maintaining euglycemia.

JCI Insight 2021 12 22;6(24). Epub 2021 Dec 22.

Molecular Signaling Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland, USA.

Glucagon, a hormone released from pancreatic α cells, plays a key role in maintaining euglycemia. New insights into the signaling pathways that control glucagon secretion may stimulate the development of novel therapeutic agents. In this study, we investigated the potential regulation of α cell function by G proteins of the Gq family. The use of a chemogenetic strategy allowed us to selectively activate Gq signaling in mouse α cells in vitro and in vivo. Acute stimulation of α cell Gq signaling led to elevated plasma glucagon levels, accompanied by increased insulin release and improved glucose tolerance. Moreover, chronic activation of this pathway greatly improved glucose tolerance in obese mice. We also identified an endogenous Gq-coupled receptor (vasopressin 1b receptor; V1bR) that was enriched in mouse and human α cells. Agonist-induced activation of the V1bR strongly stimulated glucagon release in a Gq-dependent fashion. In vivo studies indicated that V1bR-mediated glucagon release played a key role in the counterregulatory hyperglucagonemia under hypoglycemic and glucopenic conditions. These data indicate that α cell Gq signaling represents an important regulator of glucagon secretion, resulting in multiple beneficial metabolic effects. Thus, drugs that target α cell-enriched Gq-coupled receptors may prove useful to restore euglycemia in various pathophysiological conditions.
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http://dx.doi.org/10.1172/jci.insight.152852DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783673PMC
December 2021

Rutaecarpine Prevents High Glucose-Induced Endothelial Cell Senescence Through Transient Receptor Potential Vanilloid Subtype 1/ SIRT1 Pathway.

J Cardiovasc Pharmacol 2022 Jan;79(1):e129-e137

Department of Physiology, School of Basic Medicine, Jiangxi Medical College, Nanchang University, Nanchang, PR China; and.

Abstract: SIRT1 functions as a longevity factor to counteract vascular aging induced by high glucose. Our previous study revealed that rutaecarpine, the natural agonist of transient receptor potential vanilloid subtype 1 (TRPV1), prevented high glucose-induced endothelial dysfunction. The present study aims to evaluate the effects of rutaecarpine on endothelial cell senescence induced by high glucose, and focus on the regulatory effect on SIRT1 expression. In cultured human umbilical vein endothelial cell (HUVEC), exposure to 33 mM high glucose for 72 hours induced cellular senescence, demonstrated as cell cycle arrest at G0/G1 phase, decreased cell viability, and increased number of senescence-associated β-galactosidase positive senescence cells and ROS production, which were effectively attenuated by treatment with rutaecarpine (0.3, 1, and 3 μM). Furthermore, rutaecarpine upregulated longevity protein SIRT1 expression in HUVECs, accompanied by decreased level of senescence marker p21. In addition, rutaecarpine increased intracellular calcium level in HUVECs, and pretreatment with TRPV1 antagonist capsazepine, intracellular Ca2+ chelator BAPTA-AM or CaM antagonist W-7 abolished the effects of rutaecarpine on SIRT1 expression. In summary, this study shows that rutaecarpine upregulates SIRT1 expression and prevents high glucose-induced endothelial cell senescence, which is related to activation of TRPV1/[Ca2+]i/CaM signal pathway. Our findings provide evidence that rutaecarpine may be a promising candidate with a novel mechanism in prevention vascular aging in diabetes.
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http://dx.doi.org/10.1097/FJC.0000000000001166DOI Listing
January 2022

Assessment of Donor Liver Pathology Predicts Survival After Liver Transplantation: A Retrospective Cohort Study.

Transplant Proc 2021 Dec 1;53(10):2963-2970. Epub 2021 Nov 1.

Wuhan University, Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, National Quality Control Center for Donated Organ Procurement, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan Hubei, China. Electronic address:

Background: The aims of this study were to investigate the pathologic manifestation of pretransplant biopsy and to provide an accurate assessment method for liver graft of China Donation after Citizen's Death (CDCD).

Methods: A retrospective analysis was performed based on clinical and biopsy data of 96 CDCD liver transplantations completed between January 2012 and December 2017. The pretransplant pathologic sections were semiquantitatively scored according to Banff Schema recommendations on liver allograft pathology. Graft overall survival (OS) and early allograft dysfunction (EAD) rates were observed.

Results: The histologic analysis of the 96 CDCD liver graft biopsy specimens was summarized, including portal area neutrophilic infiltrate, macrovesicular steatosis, microvesicular steatosis, and hepatocellular swelling. Among these pathologic characteristics, only portal area neutrophilic infiltrate ≥20% was an independent risk factor for graft survival, although it has limited effect on the recipient's short-term prognosis.

Conclusions: We found that portal area neutrophilic infiltrate ≥20% was an independent risk factors for long-term graft survival. According to this criterion, we can identify liver transplant recipients at risk for poor prognosis and make timely interventions.
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http://dx.doi.org/10.1016/j.transproceed.2021.09.015DOI Listing
December 2021

New Mechanistic Insights into Purine Biosynthesis with Second Messenger c-di-AMP in Relation to Biofilm-Related Persistent Methicillin-Resistant Staphylococcus aureus Infections.

mBio 2021 12 2;12(6):e0208121. Epub 2021 Nov 2.

The Lundquist Institute for Biomedical Innovation, Harbor-UCLA Medical Center, Torrance, California, USA.

Persistent methicillin-resistant Staphylococcus aureus (MRSA) endovascular infections represent a significant clinically challenging subset of invasive, life-threatening S. aureus infections. We have recently demonstrated that purine biosynthesis plays an important role in such persistent infections. Cyclic di-AMP (c-di-AMP) is an essential and ubiquitous second messenger that regulates many cellular pathways in bacteria. However, whether there is a regulatory connection between the purine biosynthesis pathway and c-di-AMP impacting persistent outcomes was not known. Here, we demonstrated that the purine biosynthesis mutant MRSA strain, the Δ strain (compared to its isogenic parental strain), exhibited the following significant differences : (i) lower ADP, ATP, and c-di-AMP levels; (ii) less biofilm formation with decreased extracellular DNA (eDNA) levels and Triton X-100-induced autolysis paralleling enhanced expressions of the biofilm formation-related two-component regulatory system and its downstream gene ; (iii) increased vancomycin (VAN)-binding and VAN-induced lysis; and (iv) decreased wall teichoic acid (WTA) levels and expression of the WTA biosynthesis-related gene, . Substantiating these data, the (encoding diadenylate cyclase enzyme required for c-di-AMP synthesis) mutant strain ( strain versus its isogenic wild-type MRSA and -complemented strains) showed significantly decreased c-di-AMP levels, similar effects as seen above for the mutant and hypersusceptible to VAN treatment in an experimental biofilm-related MRSA endovascular infection model. These results reveal an important intersection between purine biosynthesis and c-di-AMP that contributes to biofilm-associated persistence in MRSA endovascular infections. This signaling pathway represents a logical therapeutic target against persistent MRSA infections. Persistent endovascular infections caused by MRSA, including vascular graft infection syndromes and infective endocarditis, are significant and growing public health threats. A particularly worrisome trend is that most MRSA isolates from these patients are "susceptible" to conventional anti-MRSA antibiotics, such as VAN and daptomycin (DAP), based on Clinical and Laboratory Standards Institute breakpoints. Yet, these antibiotics frequently fail to eliminate these infections . Therefore, the persistent outcomes in MRSA infections represent a unique and important variant of classic "antibiotic resistance" that is only disclosed during antibiotic treatment. Given the high morbidity and mortality associated with the persistent infection, there is an urgent need to understand the specific mechanism(s) of this syndrome. In the current study, we demonstrate that a functional intersection between purine biosynthesis and the second messenger c-di-AMP plays an important role in VAN persistence in experimental MRSA endocarditis. Targeting this pathway may represent a potentially novel and effective strategy for treating these life-threatening infections.
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http://dx.doi.org/10.1128/mBio.02081-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8561390PMC
December 2021

LKB1 Regulates Goat Intramuscular Adipogenesis Through Focal Adhesion Pathway.

Front Physiol 2021 13;12:755598. Epub 2021 Oct 13.

Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu, China.

Intramuscular fat (IMF) deposition is one of the most important factors to affect meat quality in livestock and induce insulin resistance and adverse metabolic phenotypes for humans. However, the key regulators involved in this process remain largely unknown. Although liver kinase B1 (LKB1) was reported to participate in the development of skeletal muscles and classical adipose tissues. Due to the specific autonomic location of intramuscular adipocytes, deposited between or within muscle bundles, the exact roles of LKB1 in IMF deposition need further verified. Here, we cloned the goat LKB1 coding sequence with 1,317 bp, encoding a 438 amino acid peptide. LKB1 was extensively expressed in detected tissues and displayed a trend from decline to rise during intramuscular adipogenesis. Functionally, knockdown of LKB1 by two individual siRNAs enhanced the intramuscular preadipocytes differentiation, accompanied by promoting lipid accumulation and inducing adipogenic transcriptional factors and triglyceride synthesis-related genes expression. Conversely, overexpression of LKB1 restrained these biological signatures. To further explore the mechanisms, the RNA-seq technique was performed to compare the difference between siLKB1 and the control group. There were 1,043 differential expression genes (DEGs) were screened, i.e., 425 upregulated genes and 618 downregulated genes in the siLKB1 group. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis predicted that the DEGs were mainly enriched in the focal adhesion pathway and its classical downstream signal, the PI3K-Akt signaling pathway. Specifically, knockdown of LKB1 increased the mRNA level of focal adhesion kinase (FAK) and in LKB1-overexpressed cells, a key component of the activated focal adhesion pathway. Convincingly, blocking this pathway by a specific FAK inhibitor (PF573228) rescued the observed phenotypes in LKB1 knockdown adipocytes. In conclusion, LKB1 inhibited goat intramuscular adipogenesis through the focal adhesion pathway. This work expanded the genetic regulator networks of IMF deposition and provided theoretical support for improving human health and meat quality from the aspect of IMF deposition.
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http://dx.doi.org/10.3389/fphys.2021.755598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548615PMC
October 2021

Results of multiple ligament reconstruction after knee dislocation--A prospective study with 95 patients and minimum 2-year follow up.

BMC Musculoskelet Disord 2021 Oct 27;22(1):904. Epub 2021 Oct 27.

Department of Orthopaedic Surgery, West China Hospital, Sichuan University, No 37 Guo Xue Xiang, Chengdu, Sichuan, 610041, P. R. China.

Background: There is still a lack of clinical data in arthroscopic treatment for treating multiple ligament injuries. This study aims to evaluate the clinical outcomes of patients with multiple ligament injuries undergoing treatment based on the classification stage and type of injury.

Methods: A prospective, clinical trial on multiple ligament injuries was planned, which included 95 patients (58 men and 37 women; age: 42.8 ± 11.9 [range, 18-63] years) from October 2017 to June 2018. Injuries were classified into three stages (emergency stage < 24 h; acute stage: 24 h to 3 weeks, and chronic stage: > 3 weeks) and six types (KD I-VI) based on injuries time and structures, which indicated appropriate treatments. The clinical outcomes were evaluated at 2, 4, 6, 8, and 12 weeks and at 6, 9, 12 months and 24 months after surgery. The final choices in efficacy index included International Knee Documentation Committee (IKDC) score, Lysholm score, visual analog scale (VAS) score, and range of motion.

Results: During the follow-up, all patients exhibited statistically significant functional improvement in the injured limb compared with their preoperative situation. The mean postoperative scores of acute stage patients at 2-year follow-up were IKDC subjective score, 77.54 ± 11.53; Lysholm score, 85.96 ± 9.39; Tegner score, 4.13 ± 1.08; and VAS score, 1.21 ± 0.76. The mean postoperative scores of chronic stage patents at 2-year follow-up were IKDC subjective score, 74.61 ± 12.38; Lysholm score, 81.71 ± 10.80; Tegner score, 3.96 ± 1.14; and VAS score, 1.71 ± 0.60. The IKDC subjective score, Lysholm score, and Tegner score were significantly improved (P < 0.01) and the VAS score was significantly decreased (P < 0.01) at 2-year follow-up. Regarding the multiple ligament injuries classification, patients with more structural damage in stages V and VI showed less progress in functional recovery than those in stages I-IV.

Conclusions: This new classification with three stages and six types helps to identify the severity of injury and plan the management effectively. The outcomes were encouraging and the subjective functional results showed significant improvement at 2-year follow-up.

Study Design: Prospective clinical trial.

Level Of Evidence: II.
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http://dx.doi.org/10.1186/s12891-021-04596-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554847PMC
October 2021

Gait analysis combined with the expression of TGF-β1, TGF-β3 and CREB during Achilles tendon healing in rat.

Chin J Traumatol 2021 Nov 12;24(6):360-367. Epub 2021 Oct 12.

Department of Orthopaedics, Daping Hospital, Army Medical University, Chongqing, 400042, China. Electronic address:

Purpose: To observe the changes of gait behavior and the expression of wound healing factors of transforming growth factor-β1 (TGF-β1), TGF-β3 and cAMP response element binding protein-1 (CREB-1) during the healing of Achilles tendon in a rat model, and to investigate whether gait analysis can be used to evaluate the tendon healing.

Methods: Achilles tendon of 40 healthy male Sprague-Dawley rats were transected and sutured to establish the Achilles tendon injury (ATI) model. They were randomly divided into 4 groups based on the observational time point at 1, 2, 4 and 6 weeks after injury (n = 10 for each group). Before modeling, 9 rats were randomly selected for CatWalk gait analysis, which contained step cycle, single stance time and average speed. Data were recorded as the normal controls. After then, ATI models were established in the left hind limbs of the all 40 rats (ATI group), while the right hind limbs were only cut and sutured without injury of the Achilles tendon (sham operation group). At 1, 2, 4 and 6 weeks after injury, the gait behavior of the corresponding group of rats (n = 9) as observed and recorded by CatWalk platform. After then, the rats were sacrificed and Achilles tendon of both limbs was harvested. The tendon healing was observed by gross anatomy and histological examination, and the protein and mRNA expression of TGF-β1, TGF-β3, CREB-1 were observed by immunohistochemistry and qPCR. The results of tendon gross grading were analyzed by Wilcoxon rank sum test, and other data were analyzed by one-way analysis of variance among multiple groups.

Results: Compared with normal controls, all gait indexes (step cycle, single stance time and average speed) were greatly affected following ATI, which however improved with time. The step cycle was significantly lower at 1, 2 and 4 weeks after ATI (compared with normal controls, all p < 0.05), but almost returned to the normal level at 6 weeks ((0.694 ± 0.102) vs. (0.503 ± 0.094) s, p > 0.05). The single stance time of the ATI group was significantly shorter at 1 and 2 weeks after operation ((0.078 ± 0.010) s at 1 week, (0.078 ± 0.020) s at 2 weeks, all p < 0.001) and revealed no significant difference at 4 weeks (p = 0.120). The average speed of ATI group at 1, 2, 4, 6 weeks was significantly lower than that in the normal control group (all p < 0.001). Gross observation showed that the grade of local scar adhesion in ATI group increased significantly at 2, 4 and 6 weeks, compared with the sham operation group (all p < 0.001). Extensive adhesion was formed at 6 weeks after ATI. The results of HE staining showed that the number of fibroblast increased gradually and arranged more orderly in ATI group at 1, 2 and 4 weeks (all p < 0.001), and decreased at 6 weeks, but it was still significantly higher than that of the sham operation group (p < 0.001). Immunohistochemistry showed that the positive expression of TGF-β1, TGF-β3, CREB-1 in ATI group was higher than that in the sham operation group at 4 time points (all p < 0.05), which reached the peak at 2 weeks after operation and decreased at 4 weeks (p = 0.002, p < 0.001, p = 0.041, respectively). The results of qPCR suggested that the mRNA expression of TGF-β1, TGF-β3, CREB-1 in ATI group was higher than that in the sham operation group at all-time points (all p < 0.05), which reached the peak at 2 weeks after operation, decreased at 4 weeks, and significantly decreased at 6 weeks (all p < 0.001).

Conclusion: Gait behavior indexes are associated with Achilles tendon healing. The study gives an insight of TGF-β1, TGF-β3, CREB-1 changes in the coursing of Achilles tendon healing and these cytokines may be able to be used to regulate the Achilles tendon healing.
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http://dx.doi.org/10.1016/j.cjtee.2021.10.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606907PMC
November 2021

The Efficacy of Moxibustion on the Serum Levels of CXCL1 and -EP in Patients with Rheumatoid Arthritis.

Pain Res Manag 2021;2021:7466313. Epub 2021 Oct 13.

Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China.

Objective: This study aims to evaluate the efficacy of moxibustion on joint swelling and pain and the levels of C-X-C motif chemokine ligand 1 (CXCL1), -endorphin (-EP) in serum of rheumatoid arthritis (RA) patients and to investigate the anti-inflammatory and analgesic mechanism of moxibustion on improving RA.

Methods: Sixty-eight patients with RA were randomly and equally classified into the control and treatment groups. The control group was treated with routine drug therapy, while the treatment group received routine drug therapy and moxibustion. Both groups were treated for eight weeks. The symptoms and laboratory indicators of RA patients were compared in the two groups before and after intervention.

Results: Sixty-one patients completed the study: four patients dropped out from the treatment group and three from the control group. Trial endpoints were change (∆) in symptoms, measured by Ritchie's articular index (RAI), swollen joint count (SJC), and laboratory indicators, measured by the level of CXCL1, -EP, tumor necrosis factor- (TNF-), and interleukin-1 (IL-1). ∆RAI, ∆SJC, ∆CXCL1, ∆-EP, ∆TNF-, and ∆IL-1 in the treatment group were superior to the control group (13.50 [14.50] versus 6.00 [13.00] in ∆RAI, 4.00 [3.00] versus 2.00 [4.00] in ∆SJC, 0.04 ± 0.79 ng/mL versus -0.01 ± 0.86 ng/mL in ∆CXCL1, -2.43 [5.52] pg/mg versus -0.04 [4.09] pg/mg in ∆-EP, 3.45 [5.90] pg/mL versus 1.55 [8.29] pg/mL in ∆TNF-, and 6.15 ± 8.65 pg/mL versus 1.28 ± 8.51 pg/mL in ∆IL-1; all  < 0.05).

Conclusion: Moxibustion can improve the joint swelling and pain symptoms in patients with RA, which may be related to the fact that moxibustion can reduce the release of inflammatory factors in patients with RA and downregulate the level of CXCL1 and increase the level of -EP at the same time. This trial is registered with ChiCTR-IOR-17012282.
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http://dx.doi.org/10.1155/2021/7466313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528578PMC
January 2022

A multifunctional hollow TPU fiber filled with liquid metal exhibiting fast electrothermal deformation and recovery.

Soft Matter 2021 Nov 17;17(44):10016-10024. Epub 2021 Nov 17.

School of Optical Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Conductive fibers have received considerable interest due to their potential applications in the flexible electronics field. Fabricating a conductive fiber that can realize fast deformation with stretchability for multifunctional applications is still highly appealing. Here, we present a deformable conductive fiber (DCF) fabricated by injecting liquid metal (LM) into a hollow thermoplastic polyurethane (TPU) fiber; the DCF can be shaped into a 2D or 3D shape by an electrothermal method at the thermoplastic transition point of TPU. Combined with the solid-liquid phase transition characteristics of the LM at its melting point, the DCF exhibits a variable shape memory feature at two transition points. We have demonstrated that the double-torsional DCF and the helical DCF can act as a capacitive sensor and an inductive sensor, respectively, and they have both been used for human motion monitoring. In addition, the helical DCF can also act as a stretchable electrode with excellent electrical properties (resistance change <2%) under a maximal mechanical strain of 3300%. Overall, the DCF presents great potential for applications in human motion monitoring, soft robotics and smart electronic textiles.
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http://dx.doi.org/10.1039/d1sm01189hDOI Listing
November 2021
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