Publications by authors named "Yan Xing"

526 Publications

Anti-cancer Peptide Recognition Based on Grouped Sequence and Spatial Dimension Integrated Networks.

Interdiscip Sci 2021 Oct 12. Epub 2021 Oct 12.

People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China.

The diversification of the characteristic sequences of anti-cancer peptides has imposed difficulties on research. To effectively predict new anti-cancer peptides, this paper proposes a more suitable feature grouping sequence and spatial dimension-integrated network algorithm for anti-cancer peptide sequence prediction called GRCI-Net. The main process is as follows: First, we implemented the fusion reduction of binary structure features and K-mer sparse matrix features through principal component analysis and generated a set of new features; second, we constructed a new bidirectional long- and short-term memory network. We used traditional convolution and dilated convolution to acquire features in the spatial dimension using the memory network's grouping sequence model, which is designed to better handle the diversification of anti-cancer peptide feature sequences and to fully learn the contextual information between features. Finally, we achieved the fusion of grouping sequence features and spatial dimensional integration features through two sets of dense network layers, achieved the prediction of anti-cancer peptides through the sigmoid function, and verified the approach with two public datasets, ACP740 (accuracy reached 0.8230) and ACP240 (accuracy reached 0.8750). The following is a link to the model code and datasets mentioned in this article: https://github.com/ YouHongfeng101/ACP-DL.
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http://dx.doi.org/10.1007/s12539-021-00481-0DOI Listing
October 2021

The association of elevated serum lipocalin-2 levels with diabetic peripheral neuropathy in type 2 diabetes.

Endocr Connect 2021 Oct 1. Epub 2021 Oct 1.

W Wang, Endocrinology, The First Affiliated Hospital of USTC, Hefei, China.

A variety of studies have demonstrated the role of lipocalin-2 (LCN2) in both diabetes and neurological disorders. Nevertheless, the relationship between LCN2 and diabetic peripheral neuropathy (DPN) needs to be elucidated in humans. Therefore, this study was aimed to investigate the association of LCN2 with DPN in type 2 diabetes (T2D). A total 207 participants with T2D and 40 participants with normal glucose tolerance (NGT) were included in this study. All participants were classified into DPN group and non-DPN (NDPN) group based on the Toronto Clinical Neuropathy Scoring (TCNS). Demographic and biochemical parameters were measured. Serum LCN2 levels were determined using an enzyme-linked immunosorbent assay method. Serum LCN2 levels in NGT group were lower than those in either DPN group (p = 0.000) or NDPN group (p = 0.043), while serum LCN2 levels in DPN group were higher than NDPN group (p = 0.001). Moreover, serum LCN2 levels positively correlated to TCNS scores, which reflects neuropathy severity (r = 0.438, p = 0.000). Multivariate stepwise regression analysis showed that BMI, triglycerides and diastolic pressure were independently associated with serum LCN2 in DPN. Additionally, logistic regression analysis demonstrated that LCN2 (OR = 1.009) and diabetes duration (OR = 1.058) were independently associated with the occurrence of DPN in T2D.Our report reveals the association of serum LCN2 with DPN in T2D. LCN2 might be used to evaluate DPN severity and serve a role in the pathogenesis of DPN.
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http://dx.doi.org/10.1530/EC-21-0290DOI Listing
October 2021

Gait characteristics and effects of the cerebrospinal fluid tap test in probable idiopathic normal pressure hydrocephalus.

Clin Neurol Neurosurg 2021 Sep 20;210:106952. Epub 2021 Sep 20.

Department of Neurology, Aviation General Hospital, 100012 Beijing, China. Electronic address:

Objectives: To evaluate gait characteristics and investigate changes pre- and post- cerebrospinal fluid tap test (CSF TT) in gait parameters in patients with probable idiopathic normal pressure hydrocephalus (iNPH).

Methods: Sixty patients were sequential circuited and diagnosed with possible iNPH according to Japanese second iNPH guidelines at our hospital from December 2016 to March 2021. All patients underwent the CSF TT. Gait parameters, cognitive and urinary function were assessed pre- and post-CSF TT. Patients who were unable to ambulate to take the tests or could not walk independently or walked normally were excluded.

Results: Twenty-six patients were diagnosed with probable iNPH using the CSF TT. After CSF TT, the Boon sum score improved from 20.0 ± 7.7-16.6 ± 8.0 (p < 0.001), the Boon walking score improved from 8.9 ± 3.5-7.8 ± 4.4 (p = 0.008), the Boon step score improved from 6.3 ± 2.3-5.2 ± 2.1 (p < 0.001), the Boon time score improved from 4.9 ± 2.4-3.7 ± 2.3 (p < 0.001), tandem walking disturbance improved from 1.7 ± 0.7-1.4 ± 0.9 (p = 0.043), tendency toward falling improved from 1.7 ± 0.7-1.3 ± 1(p = 0.022), 3-meter timed up and go test (3-mTUG) improved from 21.9 ± 7.1-17.6 ± 5.1( p < 0.001), 10-meter walking (10-MWT) step improved from 31.1 ± 13.1-24.6 ± 7.5 (p < 0.001), velocity improved from 0.7 ± 0.2-0.8 ± 0.3 (p < 0.001) and stride length improved from 0.4 ± 0.1-0.46 ± 0.1(p < 0.001), compared with before the CSF TT.

Conclusion: These results suggest that many parameters in the Boon gait test were responsive to the CSF TT, and the Boon gait test may help objectify response to the CSF TT by combining the 10-MWT and 3-mTUG gait assessments.
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http://dx.doi.org/10.1016/j.clineuro.2021.106952DOI Listing
September 2021

Health risk and disease burden attributable to long-term global fine-mode particles.

Chemosphere 2021 Oct 1;287(Pt 4):132435. Epub 2021 Oct 1.

State Key Laboratory of Earth Surface Processes and Resource Ecology, and College of Global Change and Earth System Science, Beijing Normal University, Beijing, China.

Particulate matter 2.5 (PM) pollution has long been a global environmental problem and still poses a great threat to public health. This study investigates global spatiotemporal variations in PM using the newly developed satellite-derived PM dataset from 1998 to 2018. An integrated exposure-response (IER) model was employed to examine the characteristics of PM-related deaths caused by chronic obstructive pulmonary disease (COPD), ischemic heart disease (IHD), lung cancer (LC), and stroke in adults (age≥25), as well as lower respiratory infection (LRI) in children (age≤5). The results showed that high annual PM concentrations were observed mainly in East Asia and South Asia. Over the 19-year period, PM concentrations constantly decreased in developed regions, but increased in most developing regions. Approximately 84% of the population lived in regions where PM concentrations exceeded 10 μg/m. Meanwhile, the vast majority of the population (>60%) in East and South Asia was consistently exposed to PM levels above 35 μg/m. PM exposure was linked to 3.38 (95% UI: 3.05-3.70) million premature deaths globally in 2000, a number that increased to 4.11 (95% UI: 3.55-4.69) million in 2018. Premature deaths related to PM accounted for 6.54%-7.79% of the total cause of deaths worldwide, with a peak in 2011. Furthermore, developing regions contributed to the majority (85.95%-95.06%) of PM-related deaths worldwide, and the three highest-ranking regions were East Asia, South Asia, and Southeast Asia. Globally, IHD and stroke were the two main contributors to total PM-related deaths, followed by COPD, LC, and LRI.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132435DOI Listing
October 2021

Diagnostic significance of magnetic resonance imaging in distinguishing temporomandibular disorders: a retrospective chart review.

BMC Oral Health 2021 09 28;21(1):481. Epub 2021 Sep 28.

Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, No. 95, Yongan Road, Xicheng District, Beijing, 100050, China.

Background: This study was to evaluate the diagnostic significance of Magnetic Resonance Imaging (MRI) in distinguishing temporomandibular disorders (TMD).

Methods: A total of 684 patients with TMD were included in the study. Diagnosis for TMD was conducted according to the international criteria. Two professional radiologists were selected for professional training, and the Kappa values were compared for the diagnosis results to determine the consistency of the diagnosis. Then MRI images of these 684 patients were analyzed and the diagnosis results were obtained.

Results: MRI can be used for the diagnosis of TMD. There were significantly more females (518 cases) than males (166 cases) with TMD; Disc displacement with/without reduction is more common in the youth group, with the majority aged 20-30 years. The highest incidence of temporomandibular joint osteoarthrosis is in the 60-year-old age group, followed by the 70-year-old age group.

Conclusions: Bilateral temporomandibular joint MRI can clearly show their changes; there are significantly more female with TMD than male; osteoarthritis has a significant correlation with age.
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http://dx.doi.org/10.1186/s12903-021-01826-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479910PMC
September 2021

Extended or Continuous Infusion of Carbapenems in Children with Severe Infections: A Systematic Review and Narrative Synthesis.

Antibiotics (Basel) 2021 Sep 9;10(9). Epub 2021 Sep 9.

Department of Pharmacy, Peking University Third Hospital, Beijing 100191, China.

We systematically reviewed the efficacy and safety of an extended or continuous infusion (EI/CI) versus short-term infusion (STI) of carbapenems in children with severe infections. Databases, including PubMed, Embase, the Cochrane Library, Clinicaltrials.gov, China National Knowledge Infrastructure, WanFang Data, and SinoMed, were systematically searched from their inceptions to 10 August 2020, for all types of studies (such as randomized controlled trials (RCTs), retrospective studies, and pharmacokinetic or population pharmacokinetic (PK/PPK) studies) comparing EI/CI versus STI in children with severe infection. There was no limitation on language, and a manual search was also conducted. The data were screened, evaluated, extracted, and reviewed by two researchers independently. Quantitative (meta-analysis) or qualitative analyses of the included studies were performed. Twenty studies (including two RCTs, one case series, six case reports, and 11 PK/PPK studies) were included in this review (CRD42020162845). The RCTs' quality evaluation results revealed a risk of selection and concealment bias. Qualitative analysis of RCTs demonstrated that, compared with STI, an EI (3 to 4 h) of meropenem in late-onset neonatal sepsis could improve the clinical effectiveness and microbial clearance rates, and reduce the rates of mortality; however, the differences in the incidence of other adverse events were not statistically significant. Retrospective studies showed that children undergoing an EI of meropenem experienced satisfactory clinical improvement. In addition, the results of the PK/PPK study showed that an EI (3 or 4 h)/CI of carbapenems in severely infected children was associated with a more satisfactory goal achievement rate (probability of target attainment) and a cumulative fraction of response than STI therapy. In summary, the EI/CI of carbapenems in children with severe infection has a relatively sufficient PK or pharmacodynamic (PD) basis and satisfactory efficacy and safety. However, due to the limited quantity and quality of studies, the EI/CI therapy should not be used routinely in severely infected children. This conclusion should be further verified by more high-quality controlled clinical trials or observational studies based on PK/PD theories.
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http://dx.doi.org/10.3390/antibiotics10091088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470113PMC
September 2021

P300 as a Potential Indicator in the Evaluation of Neurocognitive Disorders After Traumatic Brain Injury.

Front Neurol 2021 9;12:690792. Epub 2021 Sep 9.

Shanghai Key Laboratory of Forensic Medicine, Key Lab of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai, China.

Few objective indices can be used when evaluating neurocognitive disorders after a traumatic brain injury (TBI). P300 has been widely studied in mental disorders, cognitive dysfunction, and brain injury. Daily life ability and social function are key indices in the assessment of neurocognitive disorders after a TBI. The present study focused on the correlation between P300 and impairment of daily living activity and social function. We enrolled 234 patients with neurocognitive disorders after a TBI according to ICD-10 and 277 age- and gender-matched healthy volunteers. The daily living activity and social function were assessed by the social disability screening schedule (SDSS) scale, activity of daily living (ADL) scale, and scale of personality change following a TBI. P300 was evoked by a visual oddball paradigm. The results showed that the scores of the ADL scale, SDSS scale, and scale of personality change in the patient group were significantly higher than those in the control group. The amplitudes of Fz, Cz, and Pz in the patient group were significantly lower than those in the control group and were negatively correlated with the scores of the ADL and SDSS scales. In conclusion, a lower P300 amplitude means a greater impairment of daily life ability and social function, which suggested more severity of neurocognitive disorders after a TBI. P300 could be a potential indicator in evaluating the severity of neurocognitive disorders after a TBI.
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http://dx.doi.org/10.3389/fneur.2021.690792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458648PMC
September 2021

Berberine Acts on C/EBPβ/lncRNA Gas5/miR-18a-5p Loop to Decrease the Mitochondrial ROS Generation in HK-2 Cells.

Front Endocrinol (Lausanne) 2021 30;12:675834. Epub 2021 Aug 30.

Department of Endocrinology, The First Affiliated Hospital of University of Science and Technology of China (USTC), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Background: Berberine (BBR) has therapeutic effect on diabetic nephropathy (DN), but its molecular mechanism is not completely clear.

Methods: The DN model was established to observe the therapeutic effect of BBR. The expression levels of lncRNA Gas5 were detected by PCR. The transcriptional regulation of CCAAT enhancer binding protein beta (C/EBPβ) on Gas5 was analyzed by chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) and luciferase reporter gene assay. The targeted regulation between Gas5 and miR-18a-5p and between miR-18a-5p and C/EBPβ 3'-untranslated region (3'-UTR) was also analyzed.

Results: In HG environment, BBR decreased the mitochondrial reactive oxygen species (ROS) generation and activated the C/EBPβ expression in HK-2 cells; C/EBPβ could combine with the reaction element on the promoter of Gas5 to promote its expression. Gas5 also inhibited the miR-18a-5p expression as competing endogenous RNA (ceRNA) and reduce the negative regulatory effect of miR-18a-5p on C/EBPβ. BBR could activate C/EBPβ/peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) signal pathway, regulate mitochondrial energy metabolism, and inhibit ROS production and apoptosis by activating C/EBPβ/Gas5/miR-18a-5p positive feedback loop in HG environment. It also showed that BBR alleviated streptozotocin (STZ) induced renal injury in DN rats .

Conclusions: This study suggested that BBR could regulate the mitochondrial ROS generation by activating the positive feedback loop of C/EBPβ/Gas5/miR-18a-5p.
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http://dx.doi.org/10.3389/fendo.2021.675834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435884PMC
August 2021

LncRNA HOXA-AS2 Promotes Oral Squamous Cell Proliferation, Migration, and Invasion via Upregulating EZH2 as an Oncogene.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211039109

117878The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, P.R. China.

Oral squamous cell carcinoma (OSCC) is one of the most common types of cancer worldwide. Accumulating evidence has shown that long noncoding RNAs (lncRNAs) serve important roles in the development of OSCC. The purpose of this study was to investigate the biological function and underlying regulatory mechanism of lncRNA homeobox A cluster antisense RNA2 (HOXA-AS2) in OSCC. RT-qPCR was performed to analyze the HOXA-AS2 expressions in human immortalized oral epithelial cell (HIOEC) line, human OSCC cell lines, and plasma. The expression of HOXA-AS2 and enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) in Tca-8113 cells were knocked down or overexpressed by transfection with shRNA-HOXA-AS2 or pcDNA-EZH2, respectively. The interaction between HOXA-AS2 and EZH2 was validated by RNA immunoprecipitation assay. In addition, cell proliferation was assessed by CCK-8 and EdU assays. Cell cycle distribution was analyzed by flow cytometry. Cell migration and invasion were detected using wound healing and Transwell assays, respectively. Apoptosis was detected by TUNEL staining. The protein expression levels of cell cycle and apoptosis-related proteins were measured by western blot analysis. Compared with HIOEC cells, HOXA-AS2 expression in OSCC cells was upregulated. HOXA-AS2 knockdown significantly inhibited Tca-8113 cell proliferation, blocked the cell cycle by arresting cells in the G0/G1 phase, promoted apoptosis, and suppressed migration and invasion. In addition, HOXA-AS2 was predicted to directly target EZH2 and positively regulate EZH2 expression. EZH2 overexpression could reverse the inhibitory effect of HOXA-AS2 knockdown on the proliferation, migration, and invasion of Tca-8113 cells. In summary, the findings suggested that HOXA-AS2 may inhibit cell proliferation, invasion, and migration, induce cell cycle arrest in the G0/G1 phase, and increase cell apoptosis by targeting EZH2. The research indicated that HOXA-AS2/EZH2 axis may play a key role in the development of OSCC.
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http://dx.doi.org/10.1177/15330338211039109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445530PMC
September 2021

Sphingolipid profiles and their relationship with inflammatory factors in asthmatic patients of different sexes.

Chronic Dis Transl Med 2021 Sep 13;7(3):199-205. Epub 2021 May 13.

Department of Pulmonary and Critical Care Medicine, Peking University Third Hospital, Beijing 100191, China.

Background: Asthma is a heterogeneous disease with distinct prevalence and manifestation between sexes. This study was to identify sex-specific features of asthma via metabolomic analysis of sphingolipids.

Methods: Forty-two asthma patients (27 women and 15 men) admitted to the Peking University Third Hospital from January 2015 to December 2015 were enrolled. Peripheral venous blood was collected for metabolomic analysis by targeted liquid chromatography-mass spectrometry. Sex hormones(estradiol, progesterone, testosterone, and androstenedione) and multiple inflammatory factors (periostin, leptin, IgE, IL-4, IL-5, IL-10, IL-13, IL-17A, and IFN-γ) were also assessed. The eosinophil percentage in induced sputum was also detected. All these data were applied to comparative analysis between sexes.

Results: Testosterone was negatively related to periostin ( = -0.420,  = 0.009) and IL-5 ( = -0.540,  = 0.012), while estradiol was positively related to the blood eosinophil percentage ( = 0.384,  = 0.025). Among the eighteen species of sphingolipids detected in the 42 patients, five ceramide (Cer) species (Cer16:0, Cer:20:0, Cer22:0, Cer24:0, and Cer26:0) and one sphingomyelin (SM) species (SM38:0) were significantly higher in male than in female patients. Further investigation found that the correlation between Cer20:0 and IL-5 was positive in males ( = 0.943,  = 0.005) but negative in females ( = -0.561,  = 0.030).

Conclusions: Testosterone was negatively correlated with eosinophil inflammatory factors, but estradiol was positively correlated. Male asthma patients had higher ceramide and sphingomyelin levels than female patients. Different sexes had opposite correlations with ceramide and IL-5, respectively, suggesting that therapeutic strategies targeting ceramide should be different between sexes.
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http://dx.doi.org/10.1016/j.cdtm.2021.04.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413120PMC
September 2021

Exploring the best monochromatic energy level in dual energy spectral imaging for coronary stents after percutaneous coronary intervention.

Sci Rep 2021 09 2;11(1):17576. Epub 2021 Sep 2.

Imaging Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830011, China.

In this study, the optimal monochromatic energy level in dual-energy spectral CT required for imaging coronary stents after percutaneous coronary intervention (PCI) was explored. Thirty-five consecutive patients after PCI were examined using the dual-energy spectral CT imaging mode. The original images were reconstructed at 40-140 keV (10-keV interval) monochromatic levels. The in-stent and out-stent CT values at each monochromatic level were measured to calculate the signal-to-noise ratio(SNR) and contrast-to-noise ratio (CNR) for the vessel and the CT value difference between the in-stent and out-stent lumen (dCT (in-out)), which reflects the artificial CT number increase due to the beam hardening effect caused by the stents. The subjective image quality of the stent and in-stent vessel was evaluated by two radiologists using a 5-point scale. With the increase in energy level, the CT value, SNR, CNR, and dCT (in-out) all decreased. At 80 keV, the mean CT value in-stent reached (345.24 ± 93.43) HU and dCT (in-out) started plateauing. In addition, the subjective image quality of the stents and vessels peaked at 80 keV. The 80 keV monochromatic images are optimal for imaging cardiac patients with stents after PCI, balancing the enhancement and SNR and CNR in the vessels while minimizing the beam hardening artifacts caused by the stents.
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http://dx.doi.org/10.1038/s41598-021-97035-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413443PMC
September 2021

Intelligible Models for HealthCare: Predicting the Probability of 6-Month Unfavorable Outcome in Patients with Ischemic Stroke.

Neuroinformatics 2021 Aug 26. Epub 2021 Aug 26.

School of Science, China Pharmaceutical University, #639 Longmian Avenue, Jiangning District, Nanjing, 211198, China.

Early prediction of unfavorable outcome after ischemic stroke is significant for clinical management. Machine learning as a novel computational modeling technique could help clinicians to address the challenge. We aim to investigate the applicability of machine learning models for individualized prediction in ischemic stroke patients and demonstrate the utility of various model-agnostic explanation techniques for machine learning predictions. A total of 499 consecutive patients with Unfavorable [modified Rankin Scale (mRS) score 3-6, n = 140] and favorable (mRS score 0-2, n = 359) outcome after 6-month from ischemic stroke were enrolled in this study. Four machine learning models, including Random Forest [RF], eXtreme Gradient Boosting [XGBoost], Adaptive Boosting [Adaboost] and Support Vector Machine [SVM] were performed with the area-under-the-curve (AUC): (90.20 ± 0.22)%, (86.91 ± 1.05)%, (86.49 ± 2.35)%, (81.89 ± 2.40)%, respectively. Three global interpretability techniques (Feature Importance shows the contribution of selected features, Partial Dependence Plot aims to visualize the average effect of a feature on the predicted probability of unfavorable outcome, Feature Interaction detects the change in the prediction that occurs by varying the features after considering the individual feature effects) and one local interpretability technique (Shapley Value indicates the probability of unfavorable outcome of different instances) have been applied to present the interpretability techniques via visualization. Thereby, the current study is important for better understanding intelligible healthcare analytics via explanations for the prediction of local and global levels, and potentially reduction of the mortality of patients with ischemic stroke by assisting clinicians in the decision-making process.
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http://dx.doi.org/10.1007/s12021-021-09535-6DOI Listing
August 2021

Systematic genome-wide analysis of the ethylene-responsive ACS gene family: Contributions to sex form differentiation and development in melon and watermelon.

Gene 2021 Dec 19;805:145910. Epub 2021 Aug 19.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A & F University, Yangling 712100, China; State Key Laboratory of Vegetable Germplasm Innovation, Tianjin 300384, China. Electronic address:

Ethylene is an important regulatory phytohormone for sex differentiation and flower development. As the rate-limiting enzyme encoding genes in ethylene biosynthesis, ACS gene family has been well studied in cucumber; however, little is known in other cucurbit crops, such as melon and watermelon, which show diverse sex types in the field. Here, we identified and characterized eight ACS genes each in the genomes of melon and watermelon. According to the conserved serine residues at C-terminal, all the ACS genes could be characterized into three groups, which were supported by the exon-intron organizations and conserved motif distributions. ACS genes displayed diverse tissue-specific expression patterns among four melon and three watermelon sex types. Furthermore, a comparative expression analysis in the shoot apex identified orthologous pairs with potential functions in sex determination, e.g., ACS1s and ACS6s. All ACS orthologs in melon and watermelon exhibited similar expression patterns in monoecious and gynoecious genotypes, except for ACS11s and ACS12s. As expected, the majority of ACS genes were responsive to exogenous ethephon; however, some orthologs exhibited opposite expression patterns, such as ACS1s, ACS9s, and ACS10s. Collectively, our findings provide valuable ACS candidates related to flower development in various sex types of melon and watermelon.
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http://dx.doi.org/10.1016/j.gene.2021.145910DOI Listing
December 2021

Baicalein Enhances the Effect of Acarbose on the Improvement of Nonalcoholic Fatty Liver Disease Associated with Prediabetes via the Inhibition of Lipogenesis.

J Agric Food Chem 2021 Sep 18;69(34):9822-9836. Epub 2021 Aug 18.

School of Bioengineering, Dalian University of Technology, Dalian 116024, Liaoning, China.

Prediabetes is a prevalent metabolic disorder with multiple complications, including nonalcoholic fatty liver disease (NAFLD). In this study, we investigated the combinatorial effect of baicalein, a dietary flavonoid abundant in multiple edible plants, and acarbose on prediabetes-associated NAFLD. Baicalein and its metabolites inhibited lipogenesis (DNL), thereby decreasing lipid accumulation and hepatokine secretion in oleic acid-induced hepatocytes. Carbohydrate restriction, which mimicked the effect of acarbose, led to comparable results. The combinatorial effect of baicalein and acarbose was further verified in prediabetic mice with NAFLD. Through the 16-week intervention, baicalein and acarbose inhibited DNL and improved glucose tolerance, oxidative stress, liver histology, and hepatokine secretion, thereby ameliorating insulin resistance and NAFLD. Our study demonstrated that baicalein enhanced the effect of acarbose on improving NAFLD and explored the underlying multitarget mechanism, laying a theoretical foundation for the development of flavonoid dietary supplements for the simultaneous improvement of NAFLD and prediabetes.
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http://dx.doi.org/10.1021/acs.jafc.1c04194DOI Listing
September 2021

Early Prognosis Effect of Cellular Immune Paralysis on Brain Complications of Extracorporeal Membrane Oxygenation Children with Severe Sepsis.

Neuroimmunomodulation 2021 Aug 10:1-15. Epub 2021 Aug 10.

Pediatric Intensive Care Unit (PICU) of People's hospital of Henan Province, People's Hospital of Zhengzhou University, Zhengzhou, China.

Objective: The aim of the study was to explore the relationship between criticality, brain complications, and immune mechanisms in extracorporeal membrane oxygenation (ECMO) children with pneumonia and severe sepsis.

Methods: Patients with simple pneumonia (group I), ECMO patients with pneumonia and severe sepsis accompanied by brain complications (group II), and those without brain complication (group III) admitted to our pediatric intensive care unit were selected to be investigated. The relationship among the peripheral blood subgroups of immune cells, immune factors, adaptive immune responses, endothelial factors, and criticality and brain complications was then studied.

Results: Severe paralysis of normal immunity, excess abnormal immunity, and endothelial injury were consistent with the increase in the absolute value of base excess, lactic acid (Lac) content, and average hospitalization days and brain complications involved in group II (vs. group I). The ratio of CD63+ macrophage and CD63+ neutrophil subpopulation increased (p < 0.05); the expression levels of elastase+ neutrophil denatured subgroup (p < 0.05), the ratio of CCR2highCX3CR1low/CCR2lowCX3CR1high of macrophages and neutrophils (p < 0.0001), high-mobility group box 1 (HMGB1), YTHDF1, interleukin-17 protein and mRNA, and RAGE gene decreased to some extent (p < 0.05); the expression levels of Th1 cells chemokine CXCL9 protein and mRNA and sTIE2 protein increased to some extent (p < 0.05); the adaptive immune response of CD8+ CTL stimulated by lipopolysaccharide (LPS) was slightly enhanced (p < 0.05) in group III(vs. group II), which was consistent with the improvement of criticality, average hospitalization days, and the absence of brain complications in group III (vs. group II).

Conclusion: ECMO support with brain complication was related to the upregulation of HMGB1 and YTHDF1 protein; the decreased number of CD63+ macrophages and neutrophils; the increased denatured neutrophil subgroup, especially the upregulated ratio of CCR2highCX3CR1low/CCR2lowCX3CR1high of macrophages and neutrophils; the imbalance of Th17/Th1, LPS-specific CD8+ CTL adaptive immune response paralysis; and the reduced endothelial sTIE2 protein expression level which caused clinical deterioration and prolonged average hospitalization days.
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http://dx.doi.org/10.1159/000509808DOI Listing
August 2021

The Role of Immunotherapy to Overcome Resistance in Viral-Associated Head and Neck Cancer.

Front Oncol 2021 16;11:649963. Epub 2021 Jul 16.

Department of Medical Oncology and Therapeutics Research, City of Hope, Duarte, CA, United States.

A subset of head and neck cancers arising in the oropharynx and the nasopharynx are associated with human papillomavirus or Epstein-Barr virus. Unfortunately, limited treatment options exist once patients develop recurrent or metastatic disease in these cancers. Interest has risen in utilizing novel strategies including combination immune checkpoint inhibitors, vaccines, and adoptive cellular therapy, to improve treatment response and outcomes. Several ongoing studies are investigating the potential to overcome resistance to standard of care chemoradiation therapy with monotherapy or combination immunotherapy strategies in these viral-associated head and neck cancers.
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http://dx.doi.org/10.3389/fonc.2021.649963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322686PMC
July 2021

Development of a Tc-Labeled Single-Domain Antibody for SPECT/CT Assessment of HER2 Expression in Breast Cancer.

Mol Pharm 2021 09 30;18(9):3616-3622. Epub 2021 Jul 30.

Department of Nuclear Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 100 Haining Road, Shanghai 200080, China.

Accurate determination of human epidermal growth factor receptor 2 (HER2) expression is essential for HER2-targeted therapy in patients with cancer. HER2 expression in a complex environment, such as in a heterogeneous tumor, makes the precise assessment of the HER2 status difficult using current methods. In this study, we developed a novel Tc-labeled anti-HER2 single-domain antibody (Tc-NM-02) as a molecular imaging tracer for the noninvasive detection of HER2 expression and investigated its safety, radiation dosimetry, biodistribution, and tumor-targeting potential in 10 patients with breast cancer. Our data showed that no drug-related adverse reactions occurred. The tracer mainly accumulated in the kidneys and liver with mild uptake in the spleen, intestines, and thyroid; however, only background tracer levels were observed in other organs where primary tumors and metastases typically occurred. The mean effective dose was 6.56 × 10 mSv/MBq, and tracer uptake was visually observed in the primary tumors and metastases. A maximal standard uptake value of 1.5 was determined as a reasonable cutoff for identifying HER2 positivity using SPECT/CT imaging. Our Tc-NM-02 tracer is safe for use in breast cancer imaging, with reasonable radiation doses, favorable biodistribution, and imaging characteristics. Tc-NM-02 SPECT imaging may be an accurate and noninvasive method to detect the HER2 status in patients with breast cancer.
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http://dx.doi.org/10.1021/acs.molpharmaceut.1c00569DOI Listing
September 2021

Preclinical Mechanisms of Topical PRN473, a Bruton Tyrosine Kinase Inhibitor, in Immune-Mediated Skin Disease Models.

Immunohorizons 2021 07 29;5(7):581-589. Epub 2021 Jul 29.

Principia Biopharma Inc., a Sanofi Company, South San Francisco, CA.

The expression of Bruton tyrosine kinase (BTK) in B cells and innate immune cells provides essential downstream signaling for BCR, Fc receptors, and other innate immune cell pathways. The topical covalent BTK inhibitor PRN473 has shown durable, reversible BTK occupancy with rapid on-rate and slow off-rate binding kinetics and long residence time, resulting in prolonged, localized efficacy with low systemic exposure in vivo. Mechanisms of PRN473 include inhibition of IgE (FcεR)-mediated activation of mast cells and basophils, IgG (FcγR)-mediated activation of monocytes, and neutrophil migration. In vivo, oral PRN473 was efficacious and well tolerated in the treatment of canine pemphigus foliaceus. In this study, we evaluated in vitro selectivity and functionality, in vivo skin Ab inflammatory responses, and systemic pharmacology with topically administered PRN473. Significant dose-dependent inhibition of IgG-mediated passive Arthus reaction in rats was observed with topical PRN473 and was maintained when given 16 h prior to challenge, reinforcing extended activity with once-daily administration. Similarly, topical PRN473 resulted in significant dose-dependent inhibition of the mouse passive cutaneous anaphylaxis IgE-mediated reaction. Multiday treatment with topical PRN473 in rodents resulted in low-to-no systemic accumulation, suggesting that efficacy was mainly due to localized exposure. Reduced skin Ab inflammatory activity was also confirmed with oral PRN473. These preclinical studies provide a strong biologic basis for targeting innate immune cell responses locally in the skin, with rapid onset of action following once-daily topical PRN473 administration and minimal systemic exposure. Dose-dependent inhibition in these preclinical models of immune-mediated skin diseases support future clinical studies.
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http://dx.doi.org/10.4049/immunohorizons.2100063DOI Listing
July 2021

Semi-Empirical Prediction of Turned Surface Residual Stress for Inconel 718 Grounded in Experiments and Finite Element Simulations.

Materials (Basel) 2021 Jul 14;14(14). Epub 2021 Jul 14.

Shenyang Liming Aero-Engine (Group) Ltd., Shenyang 110862, China.

The surface residual stress after machining, especially for finishing, has a vital influence on the shape stability and fatigue life of components. The current study focuses on proposing an original empirical equation to predict turned surface residual stress for Inconel 718 material, taking tool parameters into consideration. The tool cutting-edge angle, rake angle, and inclination angle are introduced for the first time in the equation based on the Inconel 718 material turning experiments and finite element simulations. In this study, the reliability of simulation parameters' setting is firstly calibrated by comparing the residual stresses and chips of the experiments and simulations. The changing trends of turned forces, temperatures of lathe tool nose, and surface residual stress with turning parameters are analyzed. Then, the predictive equation of surface residual stress is proposed considering relationships between the back-rake angle, the side-rake angle, and the tool cutting-edge angle, rake angle, and inclination angle. Moreover, the genetic algorithm optimizes the objective function to obtain the undetermined coefficients in the prediction equation. Finally, the predicted accuracy of the surface residual stress is proven by comparing the experimental, simulation, and prediction values. The results indicate that the predictive equation of surface residual stress has a good accuracy in predicting turned surface residual stress for Inconel 718 materials. The correlation coefficient, R, and absolute average error between the predicted and the simulated value are 0.9624 and 13.40%, respectively. In the range of cutting parameters studied and experimental errors, this study provides an accurate predictive equation of turned surface residual stress for Inconel 718 materials.
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http://dx.doi.org/10.3390/ma14143937DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304668PMC
July 2021

MiRNA based tumor mutation burden diagnostic and prognostic prediction models for endometrial cancer.

Bioengineered 2021 12;12(1):3603-3620

Department of Reproduction, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.

Uterus Corpus Endometrial cancer (UCEC) is the sixth most common malignant tumor worldwide. In this research, we identified diagnostic and prognostic biomarkers to reflect patients' immune microenvironment and prognostic. Various data of UCEC patients from the TCGA database were obtained. Firstly, patients were divided into a high tumor mutation burden (TMB) level group and a low TMB level group according to the level of TMB. Then, differentially expressed miRNAs between the two groups were obtained. LASSO logistic regression analysis was used to construct a diagnostic model to predict the level of TMB. Univariate, multivariate, and LASSO regression analysis were used to construct a prognostic risk signature (PRS) to predict the prognosis of UCEC patients. Twenty-one miRNAs were used to construct a diagnostic model for predicting TMB levels. The AUC values of ROC curves for 21-miRNA-based diagnostic models were 0.911 in the training set, 0.827 in the test set, and 0.878 in the entire set. This diagnostic model showed positive correlation with TMB, PDL1 expression, and the infiltration of immune cells. In addition, three prognostic miRNAs were finally used to construct the PRS. The PRS was related to the expression of multiple immune checkpoints and the infiltration of multiple immune cells. Furthermore, the PRS can also reflect the response to some commonly used chemotherapy regimens. We have established a miRNA-based diagnostic model and a prognostic model that can predict the prognosis of UCEC patients and their response to chemotherapy and immunotherapy, thus providing valuable information on the choice of treatment regimen.
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http://dx.doi.org/10.1080/21655979.2021.1947940DOI Listing
December 2021

Candidate gene analysis of watermelon stripe pattern locus ClSP ongoing recombination suppression.

Theor Appl Genet 2021 Oct 29;134(10):3263-3277. Epub 2021 Jun 29.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A and F University, Yangling, 712100, Shaanxi, China.

Key Message: Using two segregating population, watermelon stripe pattern underlying gene ClSP was delimited to a 611.78 Kb region, consisting of four discrete haploblocks and ongoing recombination suppression. Stripe pattern is an important commodity trait in watermelon, displaying diverse types. In this study, two segregating populations were generated for genetic mapping the single dominant locus ClSP, which was finally delimited to a 611.78 Kb interval with suppression of recombination. According to polymorphism sites detected among genotypes, four discrete haploblocks were characterized in this target region. Based on reference genomes, 81 predicted genes were annotated in the ClSP interval, including seven transcription factors namely as candidate No1-No7. Meanwhile, the ortholog gene of cucumber ist responsible for the irregular stripes was considered as candidate No8. Strikingly, gene structures of No1-No5 completely varied from their reference descriptions and subsequently re-annotated. For instance, the original adjacent distribution candidates No2 and No3 were re-annotated as No2_3, while No4 and No5 were integrated as No4_5. Sequence analysis demonstrated the third polymorphism in CDS of re-annotated No4_5 resulting in truncated proteins in non-stripe plants. Furthermore, only No4_5 was down-regulated in light green stripes relative to dark green stripes. Transcriptome analysis identified 356 DEGs between dark green striped and light green striped peels, with genes involved in photosynthesis and chloroplast development down-regulated in light green stripes but calcium ion binding related genes up-regulated. Additionally, 38 DEGs were annotated as transcription factors, with the majority up-regulated in light green stripes, such as ERFs and WRKYs. This study not only contributes to a better understanding of the molecular mechanisms underlying watermelon stripe development, but also provides new insights into the genomic structure of ClSP locus and valuable candidates.
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http://dx.doi.org/10.1007/s00122-021-03891-2DOI Listing
October 2021

Phosphatase and Tensin Homolog Deleted on Chromosome Ten Knockdown Attenuates Cognitive Deficits by Inhibiting Neuroinflammation in a Mouse Model of Perioperative Neurocognitive Disorder.

Neuroscience 2021 08 21;468:199-210. Epub 2021 Jun 21.

Department of Anesthesiology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, People's Republic of China. Electronic address:

Phosphatase and tensin homolog deleted on chromosome ten (PTEN) is a crucial regulator of neuronal development, neuronal survival, axonal regeneration, and synaptic plasticity. In this study we examined the potential role of PTEN in cognitive function in a mouse model of perioperative neurocognitive disorder (PND). Adult male C57BL/6J mice received intracerebroventricular injections of small interfering RNA (siRNA) against PTEN or control siRNA 3 days prior to exploratory laparotomy (n = 8 per group). A group of healthy mice not undergoing surgery included as additional control. Barnes maze and fear conditioning tests were conducted 7 days after surgery. Mice were then sacrificed to examine the expression of PTEN, AMP-activated protein kinase (AMPK), ionized calcium binding adaptor molecule (Iba)-1, B-cell lymphoma (Bcl)-2, Bcl2-associated X protein (Bax), interleukin (IL)-1β, and tumor necrosis factor (TNF)-α in the hippocampus. The microglial activation was examined by immunohistochemistry using Iba-1 as a microglia maker. Nissl and terminal transferase deoxyuridine triphosphate nick-end labeling (TUNEL) staining were used to measure cell death and apoptosis. In comparison to the healthy controls, surgically treated mice had longer latency to identify the target box in both training and testing sessions in the Barnes maze test and shorter freezing time in the fear conditioning test. Surgically treated mice had increased expression of PTEN, AMPK, Bax, IL-1β, and TNF-α, as well as increasing number of activated microglia and apoptosis neurons in the hippocampus. PTEN knockdown significantly attenuated the behavioral deficits in Barnes maze and fear conditioning tests, as well as over-expression of PTEN, AMPK, Bax, IL-1β, and TNF-α induced by surgery. PTEN knockdown could attenuate cognitive deficits induced by trauma, likely through inhibiting the activation of microglia.
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http://dx.doi.org/10.1016/j.neuroscience.2021.06.021DOI Listing
August 2021

[Prognostic Value of Interim F-FDG PET/CT in Extranodal NK/T-Cell Lymphoma, Nasal Type].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):731-734

Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, China,E-mail:

Objective: To explore the value of interim F-FDG PET/CT in the prognosis of patients with extranodal NK/T-cell lymphoma, nasal type (ENKTL).

Methods: Twenty-one patients with ENKTL who were pathologically diagnosed at Shanghai General Hospital of Nanjing Medical University (Shanghai General Hospital) from January 2015 to December 2018 were retrospectively collected, and F-FDG PET/CT imaging was performed before and during treatment (3 weeks after 2-4 chemotherapy courses or 6 weeks after radiotherapy). The complete clinical data of the patients were followed up. The progression-free survival (PFS) and overall survival (OS) were analyzed by the Korean prognostic index (KPI), Deauville score (DS) and the maximum standard uptake reduction rate (ΔSUVmax). The independent risk factors affecting survival were evaluated by COX regression.

Results: After treatment, 11 patients had complete remission (CR), 3 had partial remission (PR), 1 had stable disease (SD), and 6 had disease progression (PD). The CR patients' △SUVmax was significantly higher than non-CR patients [(66.07±22.33)% vs (36.87±23.28)%, t=2.927, P=0.009]. Calculated from the receiver operating curve (ROC), the optimal cut-off point of ΔSUVmax was 51.45%. The median follow-up time was 32 months. Kaplan-Meier survival analysis showed that KPI, DS and ΔSUVmax had significance in predicting PFS and OS (P<0.05). COX regression analysis showed that DS was an independent risk factor affecting PFS (P<0.05), and KPI and ΔSUVmax were independent risk factors affecting OS (P<0.05).

Conclusion: Interim F-FDG PET/CT imaging has some value in the prognostic evaluation of patients with ENKTL.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.03.013DOI Listing
June 2021

Predictive Value of Cerebrospinal Fluid Biomarkers for Tap Test Responsiveness in Patients With Suspected Idiopathic Normal Pressure Hydrocephalus.

Front Aging Neurosci 2021 20;13:665878. Epub 2021 May 20.

Department of Neurology, Aviation General Hospital, Beijing, China.

The value of cerebrospinal fluid (CSF) biomarkers for assessing idiopathic normal pressure hydrocephalus (iNPH) must be determined. This prospective study aimed to reveal the correlation between CSF biomarkers and clinical symptoms of iNPH and the predictive value of these biomarkers for tap test responsiveness. Thirty-nine patients with suspected iNPH were recruited, contributed qualified CSF, and underwent a tap test and unified pre- and post-test evaluations of the neurological function. The analysis of biomarkers from the patients' CSF showed decreased levels of tau and its phosphorylated form, especially in the tap test (+) group. The responsiveness of the tap test was also related to the number of combined symptoms ( < 0.01), and a correlation was found between the end pressure or pressure difference in CSF and tap test responsiveness ( < 0.05). The results of the binary logistic regression analysis showed that (tap test responsiveness) = 1/1 + e - (-5.505 + 55.314 * ratio of p/-tau - 1.586 * numbers of combined symptoms). The combined indicators (-5.505 + 0.553 * percentage of p/-tau - 1.586 * numbers of combined symptoms) resulted in the highest sensitivity and specificity of 94.12% and 72.73%, respectively. CSF biomarkers may be assessed to judge tap test responsiveness, which is beneficial for the feasibility of a clinical application.
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http://dx.doi.org/10.3389/fnagi.2021.665878DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172576PMC
May 2021

Molecular and Clinical Features of Hospital Admissions in Patients with Thoracic Malignancies on Immune Checkpoint Inhibitors.

Cancers (Basel) 2021 May 28;13(11). Epub 2021 May 28.

Department of Medical Oncology and Therapeutics Research, City of Hope National Medical Center, Duarte, CA 91010-3000, USA.

Lung cancer patients undergoing systemic treatment with immune checkpoint inhibitors (ICIs) can lead to severe immune-related adverse events (irAEs) that may warrant immediate hospitalization. Patients with thoracic malignancies hospitalized at City of Hope while undergoing treatment with ICIs were identified. Pathology and available next-generation sequencing (NGS) data, including the programmed death-ligand 1 (PD-L1) status and clinical information, including hospitalizations, invasive procedures, and the occurrence of irAEs, were collected. Unpaired T-tests, Chi-square/Fisher's exact test, and logistic regression were used to analyze our cohort. The overall survival (OS) was calculated and compared using univariate and multivariate COX models. Ninety patients with stage IV lung cancer were admitted after ICI treatment. Of those patients, 28 (31.1%) had documented irAEs. Genomic analyses showed an enrichment of mutations ( = 5/6 vs. = 7/26, 83.3% vs. 26.9%; odds ratio (OR) (95% confidence interval (CI): 13.5 (1.7-166.1); < 0.05) and mutations ( = 4/6, 66.7% vs. = 5/26, 19.2%; OR (95% CI): 8.4 (1.3-49.3), < 0.05) in patients with irAE occurrences. Patients with somatic genomic alterations (GAs) in (median OS of 2.7 vs. 7.2 months; HR (95% CI): 3.1 (0.57-17.1); < 0.05) or (median OS of 3.0 vs. 12.4 months; HR (95% CI): 3.1 (0.70-13.8); < 0.05) demonstrated a significantly shorter OS. Patients with irAEs showed a trend toward improved OS (median OS 16.4 vs. 6.8 months, = 0.19) compared to hospitalized patients without documented irAEs. Lung cancer patients who required treatment discontinuance or interruption due to irAEs ( = 19) had significantly longer OS (median OS 18.5 vs. 6.2 months; HR (95% CI): 0.47 (0.28-0.79); < 0.05). Our results showed a significant survival benefit in lung cancer patients hospitalized due to irAEs that necessitated a treatment interruption. Patients with positive somatic GAs in and were associated with significantly worse OS compared to patients with negative GAs.
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http://dx.doi.org/10.3390/cancers13112653DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198372PMC
May 2021

Medical image segmentation using boundary-enhanced guided packet rotation dual attention decoder network.

Technol Health Care 2021 May 19. Epub 2021 May 19.

School of Educational Science, Xinjiang Normal University, Urumqi, Xinjiang, China.

Objective: The automatic segmentation of medical images is an important task in clinical applications. However, due to the complexity of the background of the organs, the unclear boundary, and the variable size of different organs, some of the features are lost during network learning, and the segmentation accuracy is low. To address these issues, This prompted us to study whether it is possible to better preserve the deep feature information of the image and solve the problem of low segmentation caused by unclear image boundaries.

Methods: In this study, we (1) build a reliable deep learning network framework, named BGRANet,to improve the segmentation performance for medical images; (2) propose a packet rotation convolutional fusion encoder network to extract features; (3) build a boundary enhanced guided packet rotation dual attention decoder network, which is used to enhance the boundary of the segmentation map and effectively fuse more prior information; and (4) propose a multi-resolution fusion module to generate high-resolution feature maps. We demonstrate the effffectiveness of the proposed method on two publicly available datasets.

Results: BGRANet has been trained and tested on the prepared dataset and the experimental results show that our proposed model has better segmentation performance. For 4 class classifification (CHAOS dataset), the average dice similarity coeffiffifficient reached 91.73%. For 2 class classifification (Herlev dataset), the prediction, sensitivity, specifificity, accuracy, and Dice reached 93.75%, 94.30%, 98.19%, 97.43%, and 98.08% respectively. The experimental results show that BGRANet can improve the segmentation effffect for medical images.

Conclusion: We propose a boundary-enhanced guided packet rotation dual attention decoder network. It achieved high segmentation accuracy with a reduced parameter number.
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http://dx.doi.org/10.3233/THC-202789DOI Listing
May 2021

[MAIAC AOD and PM Mass Concentrations Characteristics and Correlation Analysis in Beijing-Tianjin-Hebei and Surrounding Areas].

Huan Jing Ke Xue 2021 Jun;42(6):2604-2615

College of Resources Environment and Tourism, Capital Normal University, Beijing 100048, China.

Based on the MAIAC AOD and PM mass concentration data for the Beijing-Tianjin-Hebei region and surrounding areas from 2014 to 2018, the temporal and spatial differences in Aerosol Optical Depth (AOD) and PM mass concentrations were explored and their correlation was analyzed by linear regression. The results show that the daily average concentration of PM exceeds the standard for 33% and 57% of measurements based on the daily average standard values of the World Health Organization IT.1 and IT.2, respectively, indicating serious pollution levels. The annual average concentrations of PM and Terra and Aqua MAIAC AOD all show downward trends. The PM concentrations are high in winter and spring and low in summer and autumn; Terra and Aqua AOD values are high in spring and summer and low in autumn and winter. The seasonal and annual average concentrations of PM and AOD all show the regional pattern of "low in the north and high in the south". High-value areas are mainly located in southern Hebei, southwestern Shanxi, western Shandong, and northern Henan, while low-value areas are mainly located in northwestern Shanxi, northern Hebei, and eastern Shandong. The annual average concentration of PM is between 27 and 99μg·m, and the annual average AOD is between 0.20 and 0.69. The correlation between Aqua AOD and PM concentration is strong whereas and the correlations between Terra AOD, Aqua AOD, and PMvary significantly in different seasons; overall, correlations are strongest in spring and winter and weakest in summer and autumn. After vertical-humidity correction, the correlation between satellite AOD and PM data is significantly improved.
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http://dx.doi.org/10.13227/j.hjkx.202009200DOI Listing
June 2021

The protective role of YTHDF1-knock down macrophages on the immune paralysis of severe sepsis rats with ECMO.

Microvasc Res 2021 09 18;137:104178. Epub 2021 May 18.

Pediatric Intensive Care Unit (PICU) of Henan Provincial People's hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan 450003, People's Republic of China.

Objective: To examine the role of YTHDF1 knock-down macrophages on the immunity of severe sepsis rats with ECMO.

Methods: 15 SD rats were randomly allocated into 3 groups: mild sepsis (I), severe sepsis with ECMO (II), and YTHDF1 knock-down macrophages treatment groups (III). Blood biochemical indexes, different immune factors and brain changes were detected by RT-PCR, ELISA, ELISPOT and HE staining. Isolated macrophages subtypes and signal proteins were detected by flow cytometry, western blot and m6A RNA methylation test.

Results: The levels of HMGB1, RAGE, YTHDF1 and IL-17 in peripheral blood were significantly higher (p < 0.01), while the level of CXCL9 and TNF-α, and LPS-specific CD8CTL function were significantly decreased in group II compared with group I (p < 0.01). The ratio of CD63 macrophages (p < 0.05) and CD64 macrophages (p< 0.05) decreased and the level of elastase (p < 0.01) and CCR2CX3CR1/CCR2CX3CR1 (p < 0.01) of macrophages increased in group II. The above were consistent with the severity of biochemical indicators, the increasing endothelial injury factor (Ang2/Ang1), lower endothelial protective factor (sTie2), severer brain injury in group II. After YTHDF1 knock-down macrophages treatment, the above indexes' changes were opposite when Group III versus Group II through the down-regulation of m6A RNA methylation of JAK2/STAT3 (p < 0.01) and protein expression of JAK2/STAT3 (p < 0.05) in isolated macrophages.

Conclusions: YTHDF1 knock-down macrophages improved the immune paralysis of macrophages, Th1/Th17 and CTL and reduced the entry of macrophages into the brain to cause endothelial damage of severe sepsis rats with ECMO through the inhibition of HMGB1/RAGE and YTHDF1, m6A RNA methylation of JAK2/STAT3 and JAK2/STAT3 proteins expression in macrophages.
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http://dx.doi.org/10.1016/j.mvr.2021.104178DOI Listing
September 2021

Acute peritoneal dialysis is an efficient and reliable alternative therapy in preterm neonates with acute kidney injury.

Transl Pediatr 2021 Apr;10(4):893-899

Department of Pediatrics, Peking University Third Hospital, Beijing, China.

Background: This study aimed to assess the underlying causes and outcomes of acute peritoneal dialysis (APD) and the complications of PD procedure in preterm neonates with acute kidney injury (AKI).

Methods: A retrospective study of 21 preterm neonates who underwent APD in a neonatal intensive care unit (NICU) in Peking University Third Hospital between 2016 and 2019 was conducted. The demographic, clinical, biochemistry, and PD procedure--related information of the neonates was analyzed.

Results: Of the 21 preterm neonates, the average gestational age (GA) was 28.9±2.6 weeks, and the average birth weight was 1,226.7±495.3 g, and included 5 (23.8%) low-birth-weight infants (LBWIs), 7 (33.3%) very LBWIs (VLBWIs), and 9 (42.9%) extremely LBWIs (ELBWIs). The major underlying causes for APD were asphyxia (66.7%, n=14) and twin-twin transfusion syndrome (47.6%, n=10). PD procedure-related complications mainly involved inadequate drainage (n=5, 23.8%) and drainage infections (n=2, 9.5%). The median duration of PD was 3 days (range, 1 hour-20 days). Compared to pre-PD, blood urea nitrogen (BUN) and serum K levels were significantly decreased post-PD (P<0.05). After PD, edema disappeared in 77.8% (n=14/18) of patients, and 42.9% patients (n=9/21) gained normal urine output. Although 8 of the 21 (38.1%) patients died and 6 (29.6%) abandoned therapy, 7 (33.3%) patients including 1 VLBWI and 3 ELBWI survived.

Conclusions: APD is an efficient and reliable alternative route of renal replacement therapy particularly for reducing BUN and K levels in preterm neonates with AKI. APD is practicable in critically ill preterm neonates, even in LBWIs and ELBWIs.
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http://dx.doi.org/10.21037/tp-20-469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107877PMC
April 2021

The effect of nitrogen dioxide and atmospheric pressure on hospitalization risk for chronic obstructive pulmonary disease in Guangzhou, China.

Respir Med 2021 06 22;182:106424. Epub 2021 Apr 22.

School of Science, China Pharmaceutical University, #639 Longmian Avenue, Jiangning District, 211198, Nanjing, Jiangsu province, China. Electronic address:

Background: The relationship between air pollution and meteorological factors on diseases has become a research hotspot recently. Nevertheless, few studies have touched the inferences of nitrogen dioxide (NO) and atmospheric pressure (AP) on hospitalization risk for chronic obstructive pulmonary disease (COPD).

Objectives: To investigate the short-term impact of particulate air pollutants and meteorology factors on hospitalizations for COPD and quantify the corresponding risk burden of hospital admission.

Methods: In our study, COPD cases were collected from Guangzhou Panyu Central Hospital (n = 11,979) from Dec of 2013 to Jun 2019. The 24-h average temperature, relative humidity (RH), wind speed (V), AP and other meteorological data were obtained from Guangzhou Meteorological Bureau. Air pollution data were collected from Guangzhou Air Monitoring Station. The influence of different NO and AP values on COPD risk was quantified by a distributed lag nonlinear model (DLNM) combined with Poisson Regression and Time Series analysis.

Results: We found that NO had a non-linear relationship with the incidence of COPD, with an approximate "M" type, appearing at the peaks of 126 μg/m³ (RR = 1.32, 95%CI, 1.07 to 1.64) and 168 μg/m³ (RR = 1.21, 95%CI, 0.94 to 1.55), respectively. And the association between AP and COPD incidence exhibited an approximate J-shape with a peak occurring at 1035 hPa (RR = 1.16, 95% CI, 1.02 to 1.31).

Conclusions: The nonlinear relationship of NO and AP on COPD admission risk in different periods of lag can be used to establish an early warning system for diseases and reduce the possible outbreaks and burdens of COPD in a sensitive population.
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http://dx.doi.org/10.1016/j.rmed.2021.106424DOI Listing
June 2021
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