Publications by authors named "Yan Xiao"

862 Publications

Prevalence and Clinical Characteristics of Fabry Disease in Chinese Patients With Hypertrophic Cardiomyopathy.

Am J Med Sci 2021 Jan 14. Epub 2021 Jan 14.

Department of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

Background: The prevalence of Fabry disease (FD) in Chinese patients with hypertrophic cardiomyopathy (HCM) is unclear. We aimed to evaluate the prevalence, clinical characteristics, and outcomes of FD in Chinese patients with HCM.

Methods: Of 217 patients with HCM, FD probands were screened by next-generation sequencing at Fuwai Hospital. Medical data from α-galactosidase A activity, electrocardiography, echocardiography, coronary angiography, cardiac magnetic resonance, pathological examination, and follow up was analyzed.

Results: Two FD probands were observed (0.93% of patients with HCM), both of which were diagnosed with symptomatic obstructive HCM at 49 years of age. One proband had a GLA mutation (c.887T>C [p.M296T]) with a late-onset cardiac variant, which was characterized by dual ventricular hypertrophy and conduction disease with a permanent pacemaker. The other patient had a GLA mutation (c.758T>C [p.I253T]) with a classic phenotype and dual ventricular hypertrophy, atrioventricular block, renal failure, and recurrent cerebral infarction. Both probands had late gadolinium enhancement mainly in the basal segment of the inferolateral wall. Follow up revealed no exertional symptoms or outflow obstruction after surgical septal myectomy in the two probands, and stable renal function was observed after 6 months of migalastat therapy in the later one. A family study revealed six female carriers and three sudden cardiac deaths.

Conclusions: FD is not uncommon in Chinese patients with HCM. Multiple organic involvement, dual ventricular hypertrophy, and conduction disease provide clinical clues for suspected FD, and early genetic screening is necessary. Surgical septal myectomy and migalastat improve the long-term prognosis of patients with FD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.amjms.2021.01.009DOI Listing
January 2021

Characteristics of ambient ammonia and its effects on particulate ammonium in winter of urban Beijing, China.

Environ Sci Pollut Res Int 2021 Jul 3. Epub 2021 Jul 3.

National Engineering Research Center of Urban Environmental Pollution Control, Beijing Key Laboratory of Urban Atmospheric Volatile Organic Compounds Pollution Control and Application, Municipal Research Institute of Environmental Protection, Beijing, 100037, China.

To understand the characteristics of winter fine aerosol pollution in Beijing, we conducted continuous measurements of the atmospheric trace gas ammonia (NH), PM, and inorganic ions in PM at an urban site in Beijing from February 13 to March 17, 2015. The hourly average concentration of NH throughout the campaign was 15.4 ± 17.5 ppb. NH concentrations correlated well with NH in PM, indicating the dominant precursor role of NH on NH formation. The diurnal profile indicated an increase in NH concentrations during the morning rush hours, which was likely due to vehicle emissions. The mean ammonium conversion ratio (NHR) was 0.26, with the highest value of 0.32 in the afternoon. Elevated NHR, nitrate oxidation ratio (NOR), and NH coincided with the significant increase in O levels in the afternoon, indicating the large daytime formation of NHNO via photochemical reactions. Moreover, higher NHR values occurred under higher relative humidity (RH >60%) and lower temperature (0-10 °C). NHR increased during the nighttime and correlated well with RH, indicating the dominant role of heterogeneous reactions on gas-particle partitioning. The sulfate oxidation ratio (SOR) and NOR showed positive correlations with RH, which suggests that the conversions of SO to SO and NO to NO were sensitive to changes in RH. The sustained increase in SO concentrations at RH >60% suggests that RH had a higher influence on SO formation than on NO formation. As the sole precursor of NH, NH significantly enhanced daytime NHNO formation via homogeneous gas-phase reactions and also promoted sulfate formation via both homogeneous and heterogeneous reactions. Moreover, the back trajectory results inferred a high contribution of southwestern air masses to atmospheric NH and NH aerosol variations in Beijing. The result suggests the need for controlling the vehicle emissions to reduce the high levels of NH and alleviate PM pollution in winter in Beijing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-14108-wDOI Listing
July 2021

Is there a relationship between psoriasis and hepatitis C? A meta-analysis and bioinformatics investigation.

Virol J 2021 Jul 2;18(1):135. Epub 2021 Jul 2.

School of Life Science, Beijing University of Chinese Medicine, Beijing, China.

Background: The relationship between psoriasis and hepatitis C was previously controversial, so our purpose is to investigate this connection.

Methods: We conducted a systematic review of the case-control, cross-sectional and cohort studies examining the association between psoriasis and hepatitis C in PubMed, EMBASE and Cochrane library databases and investigated the overlapping genes between psoriasis targets and hepatitis C targets using bioinformatics analysis. Based on overlapping genes and hub nodes, we also constructed the protein-protein interaction (PPI) network and module respectively, followed by the pathway enrichment analysis.

Results: We included 11 publications that reported a total of 11 studies (8 cross-sectional and 3 case-control). The case-control and cross-sectional studies included 25,047 psoriasis patients and 4,091,631 controls in total. Psoriasis was associated with a significant increase of prevalent hepatitis C (OR 1.72; 95% confidence interval [CI] (1.17-2.52)). A total of 389 significant genes were common to both hepatitis C and psoriasis, which mainly involved IL6, TNF, IL10, ALB, STAT3 and CXCL8. The module and pathway enrichment analyses showed that the common genes had the potential to influence varieties of biological pathways, including the inflammatory response, cytokine activity, cytokine-cytokine receptor interaction, Toll-like receptor signaling pathway, which play an important role in the pathogenesis of hepatitis C and psoriasis.

Conclusion: Patients with psoriasis display increased prevalence of hepatitis C and the basic related mechanisms between hepatitis C and psoriasis had been preliminarily clarified.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12985-021-01606-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8252322PMC
July 2021

Antibacterial AIE polycarbonates endowed with selective imaging capabilities by adjusting the electrostaticity of the mixed-charge backbone.

Biomater Sci 2021 Jun 28. Epub 2021 Jun 28.

Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.

Combining rapid microbial discrimination with antibacterial properties, multi-functional biomacromolecules allow the timely diagnosis and effective treatment of infectious diseases. Through a two-step approach involving organocatalytic ring-opening copolymerization and thiol-ene modification, aggregation-induced emission (AIE) polycarbonates decorated with tertiary amines were prepared. After being ionized using acetic acid, the obtained cationic AIE polycarbonate with excellent water solubility showed bacteria imaging capabilities and antibacterial activities toward both Gram-positive S. aureus and Gram-negative E. coli. It was indicated via scanning electron microscope images that the bactericidal mechanism involved membrane lysis, consistent with most cationic polymers. Through further co-grafting carboxyl and tertiary amine groups, mixed-charge AIE polycarbonates were obtained. The isoelectric points of such mixed-charge AIE polycarbonates could be simply tuned based on the grafting ratio of positive and negative moieties. Compared with the cationic AIE polycarbonate, mixed-charge AIE polycarbonates allowed the rapid and selective imaging of S. aureus, but not E. coli. The selectivity probably arose from the lower binding forces between the mixed-charge AIE polycarbonates and the low-negative-charge components of the E. coli surface. Therefore, these biodegradable polycarbonates, which integrated selective bacteria imaging and antibiotic abilities, potentially suggest a precision medicine approach for infectious diseases. The overall synthesis approach and mixed-charge AIE polycarbonates provide new references for the design and application of bio-related AIE polymers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1bm00894cDOI Listing
June 2021

Liquid film-induced critical heat flux enhancement on structured surfaces.

Sci Adv 2021 Jun 25;7(26). Epub 2021 Jun 25.

Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Enhancing critical heat flux (CHF) during boiling with structured surfaces has received much attention because of its important implications for two-phase flow. The role of surface structures on bubble evolution and CHF enhancement remains unclear because of the lack of direct visualization of the liquid- and solid-vapor interfaces. Here, we use high-magnification in-liquid endoscopy to directly probe bubble behavior during boiling. We report the previously unidentified coexistence of two distinct three-phase contact lines underneath growing bubbles on structured surfaces, resulting in retention of a thin liquid film within the structures between the two contact lines due to their disparate advancing velocities. This finding sheds light on a previously unidentified mechanism governing bubble evolution on structured surfaces, which has notable implications for a variety of real systems using bubble formation, such as thermal management, microfluidics, and electrochemical reactors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/sciadv.abg4537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232909PMC
June 2021

, a new species of Begoniaceae from Guangdong, China.

PhytoKeys 2021 4;178:171-177. Epub 2021 Jun 4.

Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, 3888 Chenhua Road, Songjiang, Shanghai 201602, China Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences Shanghai China.

D.K.Tian & X.Yun Wang, , a new species in Begonia sect. Platycentrum of Begoniaceae from Shenzhen of Guangdong province, China, is described and illustrated. Morphologically, it is primarily similar to in the same section but differs by its denser hairs on leaf, petiole, and pedicel, abtuse anther apex, hairy ovary, and narrower adaxial fruit wing. Based on only one small population found to date, its conservation status is assigned to Critical Endangered according to the IUCN Red List Categories and Criteria.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3897/phytokeys.178.66462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195976PMC
June 2021

Occurrence of Powdery Mildew Caused by Erysiphe buhrii on Dianthus chinensis in Inner Mongolia, China.

Plant Dis 2021 Jun 22. Epub 2021 Jun 22.

Inner Mongolia Agricultural University, 117454, College of Horticulture and Plant Protection, Hohhot, Inner Mongolia, China;

Dianthus chinensis is widely cultivated for ornamental and medicinal use in China (Guo et al. 2017). The plant has been used in traditional Chinese medicine for the treatment of urinary problems such as strangury and diuresis (Han et al. 2015). In June and July 2020, powdery mildew-like signs and symptoms were seen on leaves of D. chinensis cultivated on the campus of Inner Mongolia Agricultural University, Hohhot city, Inner Mongolia Province, China. White powder-like masses occurred in irregular shaped lesions on both leaf surfaces and covered up to 50% of leaf area. Some infected leaves were deformed on their edges and some leaf senescence occurred. More than 40 % of plants (n = 180) exhibited these signs and symptoms. Conidiophores (n = 50) of the suspect fungus were unbranched and measured 70 to 140 µm long × 6 to 10 µm wide and had foot cells that were 25 to 48 µm long. Conidia (n = 50) were produced singly, elliptical to cylindrical shaped, 30 to 45 µm long × 12 to 19 µm wide, with length/width ratio of 2.0 to 3.2, and lacked fibrosin bodies. No chasmothecia were found. Based on these morphological characteristics, the fungus was tentatively identified as an Erysiphe sp. (Braun and Cook 2012). Fungal structures were isolated from diseased leaves and genomic DNA of the pathogen extracted utilizing the method described by Zhu et al. (2019). The internal transcribed spacer (ITS) region was amplified by PCR employing the primers PMITS1/PMITS2 (Cunnington et al. 2003) and the amplicon sequenced by Invitrogen (Shanghai, China). The sequence for the powdery mildew fungus (deposited into GenBank under Accession No. MW144997) showed 100 % identity (558/558 bp) with E. buhrii (Accession No. LC009898) that was reported on Dianthus sp. in Japan (Takamatsu et al. 2015). Pathogenicity tests were done by collecting fungal conidia from infected D. chinensis leaves and brushing them onto leaves of four healthy plants. Four uninoculated plants served as controls. Inoculated and uninoculated plants were placed in separate growth chambers maintained at 19 ℃, 65 % humidity, with a 16 h/8 h light/dark period. Nine-days post-inoculation, powdery mildew disease signs appeared on inoculated plants, whereas control plants remained asymptomatic. The same results were obtained for two repeated pathogenicity experiments. The powdery mildew fungus was identified and confirmed as E. buhrii based on morphological and molecular analysis. An Oidium sp. causing powdery mildew on D. chinensis previously was reported in Xinjiang Province, China (Zheng and Yu 1987). This, to the best of our knowledge, is the first report of powdery mildew caused by E. buhrii on D. chinensis in China (Farr and Rossman 2020). The sudden occurrence of this destructive powdery mildew disease on D. chinensis may adversely affect the health, ornamental value and medicinal uses of the plant in China. Identifying the cause of the disease will support efforts for its future control and management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-01-21-0048-PDNDOI Listing
June 2021

Pan-cancer analysis reveals an immunological role and prognostic potential of PXN in human cancer.

Aging (Albany NY) 2021 06 16;13(12):16248-16266. Epub 2021 Jun 16.

Department of Stomatology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.

Paxillin (PXN) is a protein involved in numerous physiological processes, and its presence is closely related to the occurrence and development of many types of tumors. However, no studies have analyzed PXN from a pan-cancer perspective. We analyzed expression, immune cell infiltration, prognosis, and biological function across different types of tumors included in The Cancer Genome Atlas and Gene Expression Omnibus datasets. The results showed that expression of varies in different tumors. Expression of strongly correlated with prognosis in patients with tumors; higher expression usually was linked to poor overall and disease-free survival. Expression of in breast invasive carcinoma and lymphoid neoplasm diffuse large B-cell lymphoma was related to the degree of CD8+ T-cell infiltration, and infiltration of cancer-associated fibroblasts, such as kidney renal papillary cell carcinoma and brain lower-grade glioma, was also observed in other tumors. The results of pan-cancer analysis showed that abnormal expression was related to poor prognosis, immune infiltration, and protein phosphorylation in different tumor types. Therefore, the gene may become a potential biomarker of clinical tumor prognosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.203154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266322PMC
June 2021

Association of T2285C polymorphism in PARP1 gene coding region with its expression, activity and NSCLC risk along with prognosis.

Mutagenesis 2021 Jun 16. Epub 2021 Jun 16.

Emergency Center, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.

Poly (ADP-ribose) polymerase-1(PARP1), a DNA repair gene, is the crucial player in the maintenance of genome integrity. T2285C polymorphism in coding region of PARP1 has been reported to be associated with susceptibility to tumors. We explored the relation and mechanism of T2285C polymorphism of PARP1 to its expression and activity along with risk and prognosis in NSCLC. mRNA expression was measured using qRT-PCR assay or collected from TCGA dataset. Protein expression was examined with immunoblotting assay. Genotypes were determined by PCR-RFLP and sequencing approaches. PARP1 activity was determined with enzyme activity assay. Regulation of SIRT7 to PARP1 were determined by over-expression and small interference experiment. Association of PARP1 T2285C polymorphism with NSCLC risk was evaluated via multiple logistic regression analysis. Comparison of treatment response and PFS of NSCLC patients among different genotypes or regimens was made by Chi-square test. Results indicated that mRNA and protein expression of PARP1 dramatically increased in NSCLC tissues in comparison to paired para-carcinoma tissues (P<0.05). TC/CC mutant genotypes were associated with markedly enhanced PARP1 mRNA level compared with TT genotype (P=0.011). No significant difference was discovered in PARP1 protein expression among TT, TC or CC genotypes (P>0.05). Subjects with variant allele C had higher risk of NSCLC in comparison to allele T carriers [odds ratio (OR) =1.560; P=0.000]. NSCLC patients carrying mutational TC or CC genotypes were correlated with unfavorable response to platinum-based chemotherapy (TT vs.TC vs.CC, P=0.010), and shorter PFS compared to TT genotype (TT vs.TC vs.CC, P=0.009). T2285C mutation of PARP1 resulted in the enhancement of its mRNA, but the decrease of enzyme activity in tumor cell. Overexpression of SIRT7 attenuated PARP1 expression and activity. These findings suggest the variant allele C of T2285C polymorphism of PARP1 linked to an increase of NSCLC risk, and unfavorable efficacy and prognosis of NSCLC patients with platinum-based chemotherapy, which might be associated with enhancement of its mRNA expression and the diminishment of activity. Identification of PARP1 T2285C polymorphism and mRNA expression may be the promising way for the individualized treatment of NSCLC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/mutage/geab022DOI Listing
June 2021

New possible silver lining for pancreatic cancer therapy: Hydrogen sulfide and its donors.

Acta Pharm Sin B 2021 May 31;11(5):1148-1157. Epub 2020 Oct 31.

Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.

As one of the most lethal diseases, pancreatic cancer shows a dismal overall prognosis and high resistance to most treatment modalities. Furthermore, pancreatic cancer escapes early detection during the curable period because early symptoms rarely emerge and specific markers for this disease have not been found. Although combinations of new drugs, multimodal therapies, and adjuvants prolong survival, most patients still relapse after surgery and eventually die. Consequently, the search for more effective treatments for pancreatic cancer is highly relevant and justified. As a newly re-discovered mediator of gasotransmission, hydrogen sulfide (HS) undertakes essential functions, encompassing various signaling complexes that occupy key processes in human biology. Accumulating evidence indicates that HS exhibits bimodal modulation of cancer development. Thus, endogenous or low levels of exogenous HS are thought to promote cancer, whereas high doses of exogenous HS suppress tumor proliferation. Similarly, inhibition of endogenous HS production also suppresses tumor proliferation. Accordingly, HS biosynthesis inhibitors and HS supplementation (HS donors) are two distinct strategies for the treatment of cancer. Unfortunately, modulation of endogenous HS on pancreatic cancer has not been studied so far. However, HS donors and their derivatives have been extensively studied as potential therapeutic agents for pancreatic cancer therapy by inhibiting cell proliferation, inducing apoptosis, arresting cell cycle, and suppressing invasion and migration through exploiting multiple signaling pathways. As far as we know, there is no review of the effects of HS donors on pancreatic cancer. Based on these concerns, the therapeutic effects of some HS donors and NO-HS dual donors on pancreatic cancer were summarized in this paper. Exogenous HS donors may be promising compounds for pancreatic cancer treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.apsb.2020.10.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144891PMC
May 2021

Combating Ambiguity for Hash-code Learning in Medical Instance Retrieval.

IEEE J Biomed Health Inform 2021 May 21;PP. Epub 2021 May 21.

When encountering a dubious diagnostic case, medical instance retrieval can help radiologists make evidence-based diagnoses by finding images containing instances similar to a query case from a large image database. The similarity between the query case and retrieved similar cases is determined by visual features extracted from pathologically abnormal regions. However, the manifestation of these regions often lacks specificity, i.e., different diseases can have the same manifestation, and different manifestations may occur at different stages of the same disease. To combat the manifestation ambiguity in medical instance retrieval, we propose a novel deep framework called Y-Net, encoding images into compact hash-codes generated from convolutional features by feature aggregation. Y-Net can learn highly discriminative convolutional features by unifying the pixel-wise segmentation loss and classification loss. The segmentation loss allows exploring subtle spatial differences for good spatial-discriminability while the classification loss utilizes class-aware semantic information for good semantic-separability. As a result, Y-Net can enhance the visual features in pathologically abnormal regions and suppress the disturbing of the background during model training, which could effectively embed discriminative features into the hash-codes in the retrieval stage. Extensive experiments on two medical image datasets demonstrate that Y-Net can alleviate the ambiguity of pathologically abnormal regions and its retrieval performance outperforms the state-of-the-art method by an average of 9.27% on the returned list of 10.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/JBHI.2021.3082531DOI Listing
May 2021

Divergence of stem biomechanics and hydraulics between lianas and trees.

AoB Plants 2021 Jun 8;13(3):plab016. Epub 2021 Apr 8.

CAS Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan 666303, China.

Liana abundance and biomass are increasing in neotropical and Asian tropical seasonal forests over the past decades. Stem mechanical properties and hydraulic traits influence the growth and survival of plants, yet the differences in stem mechanical and hydraulic performance between congeneric lianas and trees remain poorly understood. Here, we measured 11 stem mechanical and hydraulic traits for 10 liana species and 10 tree species from grown in a tropical common garden. Our results showed that lianas possessed lower stem mechanical strength as indicated by both modulus of elasticity and modulus of rupture, and higher stem potential hydraulic conductivity than congeneric trees. Such divergence was mainly attributed to the differentiation in liana and tree life forms. Whether the phylogenetic effect was considered or not, mechanical strength was positively correlated with wood density, vessel conduit wall reinforcement and sapwood content across species. Results of principle component analysis showed that traits related to mechanical safety and hydraulic efficiency were loaded in the opposite direction, suggesting a trade-off between biomechanics and hydraulics. Our results provide evidence for obvious differentiation in mechanical demand and hydraulic efficiency between congeneric lianas and trees.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/aobpla/plab016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114228PMC
June 2021

Multilayer Double-Sided Microstructured Flexible Iontronic Pressure Sensor with a Record-wide Linear Working Range.

ACS Sens 2021 05 5;6(5):1785-1795. Epub 2021 May 5.

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.

Wearable electronics, electronic skins, and human-machine interfaces demand flexible sensors with not only high sensitivity but also a wide linear working range. The latter remains a great challenge and has become a big hurdle for some of the key advancements imperative to these fields. Here, we present a flexible capacitive pressure sensor with ultrabroad linear working range and high sensitivity. The dielectric layer of the sensor is composed of multiple layers of double-sided microstructured ionic gel films. The multilayered structure and the gaps between adjacent films with random topography and size enhance the compressibility of the sensor and distribute the stress evenly to each layer, enabling a linear working range from 0.013 to 2063 kPa. Also, the densely distributed protrusive microstructures in the electric double layer contribute to a sensitivity of 9.17 kPa for the entire linear working range. For the first time, a highly sensitive pressure sensor that can measure loading conditions across 6 orders of magnitude is demonstrated. With the consistent and stable performance from a low- to high-measurement range, the proposed pressure sensor can be used in many applications without the need for recalibration to suit different loading scenarios.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acssensors.0c02547DOI Listing
May 2021

Lubricant-Infused Surfaces for Low-Surface-Tension Fluids: The Extent of Lubricant Miscibility.

ACS Appl Mater Interfaces 2021 May 5;13(19):23121-23133. Epub 2021 May 5.

Department of Mechanical Science and Engineering, University of Illinois, Urbana, Illinois 61801, United States.

Lubricant-infused surfaces (LISs) and slippery liquid-infused porous surfaces (SLIPSs) have shown remarkable success in repelling low-surface-tension fluids. The atomically smooth, defect-free slippery surface leads to reduced droplet pinning and omniphobicity. However, the presence of a lubricant introduces liquid-liquid interactions with the working fluid. The commonly utilized lubricants for LISs and SLIPSs, although immiscible with water, show various degrees of miscibility with organic polar and nonpolar working fluids. Here, we rigorously investigate the extent of miscibility by considering a wide range of liquid-vapor surface tensions (12-73 mN/m) and different categories of lubricants having a range of viscosities (5-2700 cSt). Using high-fidelity analytical chemistry techniques including X-ray photoelectron spectroscopy, nuclear magnetic resonance, thermogravimetric analysis, and two-dimensional gas chromatography, we quantify lubricant miscibility to parts per billion accuracy. Furthermore, we quantify lubricant concentrations in the collected condensate obtained from prolonged condensation experiments with ethanol and hexane to delineate mixing and shear-based lubricant drainage mechanisms and to predict the lifetime of LISs and SLIPSs. Our work not only elucidates the effect of lubricant properties on miscibility with various fluids but also develops guidelines for developing stable and robust LISs and SLIPSs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c02716DOI Listing
May 2021

Polyethylene glycol-20k reduces post-resuscitation cerebral dysfunction in a rat model of cardiac arrest and resuscitation: A potential mechanism.

Biomed Pharmacother 2021 Jul 1;139:111646. Epub 2021 May 1.

Department of Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, Shaanxi 710061, China; Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA; Department of Emergency Medicine, Virginia Commonwealth University Health System, Richmond, VA, USA. Electronic address:

Out-of-hospital cardiac arrest (CA) is a leading cause of death in the United States. Severe post-resuscitation cerebral dysfunction is a primary cause of poor outcome. Therefore, we investigate the effects of polyethylene glycol-20k (PEG-20k), a cell impermeant, on post-resuscitation cerebral function. Thirty-two male Sprague-Dawley rats were randomized into four groups: 1) Control; 2) PEG-20k; 3) Sham control; 4) Sham with PEG-20k. To investigate blood brain barrier (BBB) permeability, ten additional rats were randomized into two groups: 1) CPR+Evans Blue (EB); 2) Sham+EB. Ventricular fibrillation was induced and untreated for 8 min, followed by 8 min of CPR, and resuscitation was attempted by defibrillation. Cerebral microcirculation was visualized at baseline, 2, 4 and 6 h after return of spontaneous circulation (ROSC). Brain edema was assessed by comparing wet-to-dry weight ratios after 6 h. S-100β, NSE and EB concentrations were analyzed to determine BBB permeability damage. For results, Post-resuscitation cerebral microcirculation was impaired compared to baseline and sham control (p < 0.05). However, dysfunction was reduced in animals treated with PEG-20k compared to control (p < 0.05). Post-resuscitation cerebral edema as measured by wet-to-dry weight ratio was lower in PEG-20k compared to control (3.23 ± 0.5 vs. 3.36 ± 0.4, p < 0.05). CA and CPR increased BBB permeability and damaged neuronal cell with associated elevation of S-100β sand NSE serum levels. PEG-20k administered during CPR improved cerebral microcirculation and reducing brain edema and injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2021.111646DOI Listing
July 2021

NF-κB-Induced Upregulation of miR-146a-5p Promoted Hippocampal Neuronal Oxidative Stress and Pyroptosis TIGAR in a Model of Alzheimer's Disease.

Front Cell Neurosci 2021 16;15:653881. Epub 2021 Apr 16.

Department of Neurosurgery, West-China Hospital, Sichuan University, Chengdu, China.

Alzheimer's disease (AD) is a common neurodegenerative disorder that places a heavy burden on patients and society. Hippocampal neuronal loss is a hallmark of AD progression. Therefore, understanding the mechanism underlying hippocampal neuronal death would be of great importance for the diagnosis and treatment of AD. This study aimed to explore the molecular mechanism which nuclear factor kappa β (NF-κB) promotes hippocampal neuronal oxidative stress and pyroptosis in AD. We collected serum samples from 101 healthy elderly people and 112 patients with AD at the Affiliated Hospital of Kunming University of Science and Technology between January 2017 and January 2020. Commercially available human hippocampal neurons (HHNs) were used to establish an AD model (AD-HHN) following Aβ25-35 treatment. The mRNA expression levels of NF-κB and pyroptosis markers [NLR family pyrin domain-containing 3, caspase-1, interleukin (IL)-1β, and interleukin-18] mRNA and the expression level of miR-146a-5p in the serum samples of patients with AD and AD-HHNs were determined by quantitative reverse transcription polymerase chain reaction. Oxidative stress indices (reactive oxygen species, malondialdehyde, nicotinamide adenine dinucleotide phosphate, superoxide dismutase, glutathione, and catalase) were measured by Enzyme-Linked Immunosorbent Assay (ELISA). The expression of proteins [NF-κB, TP53-induced glycolysis and apoptosis regulator (TIGAR), and pyroptosis markers] was tested by western blotting. The relationship between miR-146a-5p and TIGAR was investigated using a dual luciferase reporter gene assay. We found that NF-κB and miR-146a-5p were highly expressed, while TIGAR was low expressed in patients with AD and AD-HHNs. In addition, there was a significant positive correlation between the expression levels of NF-κB and miR-146a-5p, but a negative correlation between NF-κB mRNA and TIGAR mRNA in patients with AD, as well as miR-146a-5p and TIGAR mRNA in patients with AD. In AD-HNNs, miR-146a-5p targeted and downregulated the expression of TIGAR. Knockdown of NF-κB or overexpression of TIGAR markedly attenuated oxidative stress and pyroptosis in AD-HHNs, while concurrent overexpression of miR-146a-5p inhibited these effects. In conclusion, NF-κB-induced upregulation of miR-146a-5p promoted oxidative stress and pyroptosis in AD-HNNs by targeting TIGAR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fncel.2021.653881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085266PMC
April 2021

Solar anti-icing surface with enhanced condensate self-removing at extreme environmental conditions.

Proc Natl Acad Sci U S A 2021 May;118(18)

Institute of Chemistry, University of Chinese Academy of Sciences, 100190 Beijing, China.

The inhibition of condensation freezing under extreme conditions (i.e., ultra-low temperature and high humidity) remains a daunting challenge in the field of anti-icing. As water vapor easily condensates or desublimates and melted water refreezes instantly, these cause significant performance decrease of most anti-icing surfaces at such extreme conditions. Herein, inspired by wheat leaves, an effective condensate self-removing solar anti-icing/frosting surface (CR-SAS) is fabricated using ultrafast pulsed laser deposition technology, which exhibits synergistic effects of enhanced condensate self-removal and efficient solar anti-icing. The superblack CR-SAS displays superior anti-reflection and photothermal conversion performance, benefiting from the light trapping effect in the micro/nano hierarchical structures and the thermoplasmonic effect of the iron oxide nanoparticles. Meanwhile, the CR-SAS displays superhydrophobicity to condensed water, which can be instantly shed off from the surface before freezing through self-propelled droplet jumping, thus leading to a continuously refreshed dry area available for sunlight absorption and photothermal conversion. Under one-sun illumination, the CR-SAS can be maintained ice free even under an ambient environment of -50 °C ultra-low temperature and extremely high humidity (ice supersaturation degree of ∼260). The excellent environmental versatility, mechanical durability, and material adaptability make CR-SAS a promising anti-icing candidate for broad practical applications even in harsh environments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.2100978118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106333PMC
May 2021

The combined use of arbuscular mycorrhizal fungi, biochar and nitrogen fertilizer is most beneficial to cultivate Cichorium intybus L. in Cd-contaminated soil.

Ecotoxicol Environ Saf 2021 Jul 23;217:112154. Epub 2021 Apr 23.

College of Agro-grassland Science, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address:

We assessed Cichorium intybus L. (chicory) growth, nutrients and Cd uptake in the presence of arbuscular mycorrhizal fungi (AMF), biochar (BC) and nitrogen (N) fertilizer applications in experimental pot studies. Mycorrhizal colonization was improved by BC addition but not N addition. However, in the presence of AMF, BC and N had positive effects on shoot biomass and plants grown in the presence of all 3 components yielded the highest levels of shoot biomass compared to pair-wise combinations or individual components singly added. AMF inoculation also increased root biomass and this effect was apparent in the combinations that included AMF without BC. Fungal inoculation also led to increased contents of phosphorus (P), magnesium (Mg) and copper (Cu) in both chicory shoots and roots and potassium (K) and manganese (Mn) in roots. Moreover, in the presence of BC, AMF inoculation evidently enhanced the shoot Ca content. In contrast, the presence of AMF inhibited plant Cd absorption and BC addition further inhibited root Cd concentration following the AMF inoculation. Applying N fertilizer alone increased Cd transfering from soil to plants. However, when combined with use of AMF and BC, Cd toxicity to plants was reduced. This study demonstrated that AMF inoculation combined with BC and N fertilizer could improve chicory growth, nutrient absorption and reduction of Cd uptake in Cd-contaminated soil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2021.112154DOI Listing
July 2021

Combined Therapy With Polyethylene Glycol-20k and MCC950 Preserves Post-Resuscitated Myocardial Function in a Rat Model of Cardiac Arrest and Cardiopulmonary Resuscitation.

J Am Heart Assoc 2021 May 22;10(9):e019177. Epub 2021 Apr 22.

Weil Institute of Emergency and Critical Care Research Virginia Commonwealth University Richmond VA.

Background To investigate the therapeutic potential of combined therapy with polyethylene glycol-20k (PEG-20k) and MCC950 on post-resuscitation myocardial function in a rat model of cardiac arrest. Methods and Results Thirty rats were randomized into 5 groups: Sham, Control, PEG-20k, MCC950, PEG-20k+ MCC950. Except for sham, animals were subjected to 6 minutes of ventricular fibrillation followed by 8 minutes cardiopulmonary resuscitation. Two milliliters PEG-20k was administered by intravenous injection coincident with the start of cardiopulmonary resuscitation; MCC950 (10 mg/kg), a highly selective NLRP3 inflammasome inhibitor, was delivered immediately after restoration of spontaneous circulation. Myocardial function, sublingual microcirculation, mitochondrial function, plasma cardiac troponin I, and interleukin-1β, expression of proteins in SIRT1 (sirtuin 1)/PGC-1α (peroxisome proliferator-activated receptor gamma coactivator 1-alpha) and NLRP3 (the NOD-like receptor family protein 3) inflammasome pathways were evaluated. Following cardiopulmonary resuscitation, myocardial function was compromised with a significantly decreased cardiac output, ejection fraction, and increased myocardial performance index, cardiac troponin I. Sublingual microcirculation was disturbed with impaired perfused vessel density and microvascular flow index. Cardiac arrest reduced mitochondrial routine respiration, Complex I-linked respiration, respiratory control rates and oxidative phosphorylation coupling efficiency. PEG-20k or MCC950 alone restored mitochondrial respiratory function, restituted sublingual microcirculation, and preserved myocardial function, whereas a combination of PEG-20k and MCC950 further improved these aspects. PEG-20k restored the expression of SIRT1 and PGC-1α, and blunted activation of NLRP3 inflammasomes. MCC950 suppressed expression of cleaved-caspase-1/pro-caspase-1, ASC (apoptosis-associated speck-like protein), GSDMD [gasdermin d], and interleukin-1β. Conclusions Combined therapy with PEG-20k and MCC950 is superior to either therapy alone for preserving post-resuscitated myocardial function, restituting sublingual microcirculation at restoration of spontaneous circulation at 6 hours. The responsible mechanisms involve upregulated expression of SIRT1/PGC1-α in tandem with inhibition of NLRP3 inflammasomes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1161/JAHA.120.019177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200739PMC
May 2021

Functional analysis and expression patterns of members of the FLOWERING LOCUS T (FT) gene family in Lilium.

Plant Physiol Biochem 2021 Jun 5;163:250-260. Epub 2021 Apr 5.

Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing, 100083, China. Electronic address:

Lilium is an important commercial flowering species, and there are many varieties and more than 100 species of wild Lilium. Lilium × formolongi is usually propagated from seedlings, and the flowering of these plants is driven mainly by the photoperiodic pathway. Most of the other lily plants are propagated via bulblets and need to be vernalized; these plants can be simply divided into pretransplantation types and posttransplantation types according to the time at which the floral transition occurs. We identified three Lilium FLOWERING LOCUS T (LFT) family members in 7 Lilium varieties, and for each gene, the coding sequence of the different varieties was identical. Among these genes, the LFT1 gene of Lilium was most homologous to the AtFT gene, which promotes flowering in Arabidopsis. We analyzed the expression patterns of LFT genes in Lilium × formolongi seedlings and in different Lilium varieties, and the results showed that LFT1 and LFT3 may promote floral induction. Compared with LFT3, LFT1 may have a greater effect on floral induction in Lilium, which is photoperiod sensitive, while LFT3 may play a more important role in the floral transition of lily plants, which have a high requirement for vernalization. LFT2 may be involved in the differentiation of bulblets, which was verified by tissue culture experiments, and LFT1 may have other functions involved in promoting bulblet growth. The functions of LFT genes were verified by the use of transgenic Arabidopsis thaliana plants, which showed that both the LFT1 and LFT3 genes can promote early flowering in Arabidopsis. Compared with LFT3, LFT1 promoted flowering more obviously, and thus, this gene could be an important promoter of floral induction in Lilium.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plaphy.2021.03.056DOI Listing
June 2021

Solid phase extraction of 16 polycyclic aromatic hydrocarbons from environmental water samples by π-hole bonds.

J Chromatogr A 2021 May 15;1645:462067. Epub 2021 Mar 15.

School of Public Health, Shanxi Medical University, Taiyuan 030001, China. Electronic address:

Through theoretical computation, it was demonstrated that perfluorobenzene can form π-hole⋅⋅⋅π bonds with polycyclic aromatic hydrocarbons (PAHs). Then, the π-hole bond was firstly introduced in solid phase extraction in which perfluorobenzene-bonded silica sorbent was synthesized and used for the solid phase extraction of sixteen PAHs in water. Compared with the traditional octadecyl silica sorbent, the perfluorobenzene-bonded silica sorbent showed higher adsorbabilities for the PAHs with 4-6 benzene rings, for which the recoveries increased by approximately 20%. Under the optimized conditions, the proposed SPE-HPLC-FLD/UV method was successfully applied for the analysis of 16 PAHs in river water and waste water samples with the limits of detection ranged from 0.002 to 0.08 μg⋅L. In addition, when the perfluorobenzene-bonded silica sorbent compared with the phenyl-bonded silica sorbent, the results indicated that π-hole⋅⋅⋅π bonds between perfluorobenzene and PAHs were stronger than the π-π interactions between the PAHs and benzene in hexane solution, which highlights the remarkable potential for the application of the π-hole bond in the SPE field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chroma.2021.462067DOI Listing
May 2021

Effects of moxibustion for COVID-19 convalescence: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Apr;100(14):e25389

Shandong University of Traditional Chinese Medicine.

Background: Coronavirus disease 2019 (COVID-19) is still spreading around the world. Moxibustion, as a significant therapy in traditional Chinese medicine (TCM), has been widely used to treat COVID-19, especially in recovery period. The study will aim to assess the efficacy and safety of moxibustion for COVID-19 convalescence.

Methods: We will systematically search the relevant randomized controlled trials in the 7 databases from inception to February 2021, including PubMed, MEDLINE, Embase, Cochrane Clinical Trials Database, Web of Science, China National Knowledge Infrastructure and Chinese Biomedical Literature Database. No language and publication status restrictions will be applied. Two reviewers will independently conduct and screen all included studies and the meta-analysis will be performed with RevMan V5.3 (The Cochrane Collaboration, Oxford, England).

Results: The study will provide a high-quality convincing assessment of the efficacy and safety of moxibustion for the treatment of COVID-19 convalescence, which will be published in a peer-reviewed journal.

Conclusion: Our study will give more comprehensive evidence of the effectiveness of moxibustion for COVID-19 convalescence.

Trial Registration Number: CRD42021230364.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036045PMC
April 2021

Adherence to National Comprehensive Cancer Network ALK Testing Guidelines for Patients with Advanced Non-Small Cell Lung Cancer in U.S. Community Medical Centers.

Oncologist 2021 06 5;26(6):e1050-e1057. Epub 2021 May 5.

University of Mississippi Medical Center, Jackson, Mississippi, USA.

Background: National Comprehensive Cancer Network (NCCN) guidelines recommend biomarker testing as the first step in the management of patients with advanced non-small cell lung cancer (aNSCLC). We assessed anaplastic lymphoma kinase (ALK) testing rates and factors related to underuse in community medical systems between 2012 and 2019 to understand guideline adoption.

Methods: A retrospective observational study using a nationwide electronic health record (EHR)-derived deidentified database was conducted. Patients with aNSCLC diagnosed in community medical centers from January 2012 to May 2019 were included to describe the ALK testing trend. This cohort was further restricted to patients diagnosed after 2015 to understand factors associated with testing underuse using mixed-effects multivariable logistic regression models.

Results: Trends for increased ALK testing rates by year were observed in both NCCN guideline-eligible patients (59.5% in 2012 to 84.1% in 2019) and -ineligible patients (15.6% to 50.8%) in a cohort of 41,728 patients. Histology type and smoking status had the greatest impact on test use. Compared with patients with nonsquamous histology and no smoking history, patients with squamous histology and no smoking history (adjusted odds ratio [aOR], 7.6; 95% confidence interval [CI], 5.6-10.4), NSCLC histology not otherwise specified (NOS) with smoking history (aOR, 3.4; 95% CI, 2.8-4.2); NSCLC NOS/nonsmoker (aOR, 1.8; 95% CI, 1.1-3.2), and nonsquamous/smoker (aOR, 1.5; 95% CI, 1.3-1.7) were less likely to be tested. Factors related to underuse also included Eastern Cooperative Oncology Group performance status, stage at initial diagnosis, and demographics.

Conclusion: This analysis of real-world data shows increasing test use by year; however, one fifth of patients eligible for ALK testing still remain untested and potentially missing therapeutic options.

Implications For Practice: Advancement in treatment of lung cancer is accompanied by an increasing number of tests that should be run to determine potential therapy options for each patient. This study assessed adoption of testing recommendations for anaplastic lymphoma kinase rearrangements in a national database. Although test use increased over the time period studied (2012-2019), there is still room for improvement. Efforts are needed to increase test use in undertested groups, thus enabling eligible patients to benefit from novel lung cancer therapies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/onco.13779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176985PMC
June 2021

Asymmetric Bubble Formation at Rectangular Orifices.

Langmuir 2021 Apr 2;37(14):4302-4307. Epub 2021 Apr 2.

Mechanical Science and Engineering, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, United States.

Bubble formation in liquids is frequently observed in nature and applied in various industrial processes. These include pool and flow boiling for thermal management systems, where bubbles may form asymmetrically at narrow slits and in convective flows. While previous studies have focused on symmetric bubble formation at circular orifices, the dynamics of asymmetric bubble formation remains poorly understood. Here, we experimentally investigate bubble formation at rectangular orifices and examine the effects of the orifice size and aspect ratio and the gas flow rate on the bubble size. The asymmetric bubble shape evolution at the rectangular orifice is analyzed, and we find that the size of the bubble neck is controlled either by the orifice size or by the capillary length. Based on these findings, we develop a static force balance model to predict the bubble size in the quasi-static regime, where the roles of Bond number and aspect ratio are identified. The bubble size evolution in the dynamic regime is further understood by introducing a Weber number that evaluates the effect of the virtual mass force induced by gas flow. Our study provides physical understanding of the dynamics of asymmetric bubble formation and guidance to predict the bubble size at asymmetric orifices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.1c00287DOI Listing
April 2021

Tools for Distributed Teamwork and Rapid Adaptation to Change: COVID-19 and Frontline Learning.

Authors:
Yan Xiao

Jt Comm J Qual Patient Saf 2021 05 6;47(5):273-274. Epub 2021 Mar 6.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcjq.2021.03.003DOI Listing
May 2021

The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O.

Front Vet Sci 2021 25;8:633706. Epub 2021 Feb 25.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.

Foot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious and economically devastating viral disease of cloven-hoofed animals worldwide. In this study, the coexpression of small ubiquitin-like modifier (SUMO)-fused capsid proteins of FMDV serotype O by single plasmid in was achieved with an optimal tandem permutation (VP0-VP3-VP1), showing a protein yield close to 1:1:1. After SUMO removal at a low level of protease activity (5 units), the assembled FMDV virus-like particles (VLPs) could expose multiple epitopes and have a size similar to the naive FMDV. Immunization of pigs with the FMDV VLPs could induce FMDV-specific humoral and cellular immune responses effectively, in a dose-dependent manner. These data suggested that the stable FMDV VLPs with multiple epitope exposure were effective for the induction of an immune response in pigs, which laid a foundation for the further development of the FMDV subunit vaccine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fvets.2021.633706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947224PMC
February 2021

Chinese Medicine Involving Triple Rehabilitation Therapy () for Knee Osteoarthritis in 696 Outpatients: A Multi-Center, Randomized Controlled Trial.

Chin J Integr Med 2021 Mar 12. Epub 2021 Mar 12.

Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.

Objective: To determine the effects of Chinese medicine (CM) involving triple rehabilitation therapy () on the progression of knee osteoarthritis (KOA).

Methods: A total of 722 patients recruited from 38 community health service centers located in China from March 2013 to March 2017 were randomly divided into treatment and control groups equally, using a cluster randomization design. Health education combined with CM involving triple rehabilitation therapy for KOA (electro-acupuncture, Chinese medicinal herb fumigating-washing, and traditional exercises) was administered in the treatment group while conventional rehabilitation therapy (physical factor therapy, joint movement training, and muscle strength training) was administered in the control group. Patients with a visual analog scale (VAS) scores ≽4 were treated with dispersible meloxicam tablets (7.5 mg, once daily). The Lequesne index scores, VAS scores, range of motion (ROM), lower limb muscle strength, knee joint circumference, quantitative scores of KOA symptoms, and the short-form 36 item health survey questionnaire (SF-36) scores were measured for each patient at 5 checkpoints (before treatment, at the 2nd week and the 4th week during the 4-week treatment period, at 1 month and 3 months after end of treatment), and adverse reactions were observed also.

Results: A total of 696 patients completed the entire process, with 351 in the treatment group and 345 in the control group. At all treatment checkpoints, the treatment group demonstrated better outcomes than the control group with regard to the total Lequesne index scores, effective rate and improvement rate of the total Lequesne index scores, VAS scores, lower limb muscle strength, knee circumference, quantitative scores of KOA symptoms, and SF-36 scores as well (P<0.05 or P<0.01). No adverse reactions were encountered in this study.

Conclusions: CM involving triple rehabilitation therapy can alleviate KOA-related pain and swelling, improve lower limb muscle strength, promote flexion and activity of the knee and improve the quality of life in patients undergoing KOA. It is suitable for patients with early or mid-stage KOA. (Registration No. ChiCTR-TRC-12002538).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11655-021-3488-6DOI Listing
March 2021

Isolation and identification of Candida tropicalis in sows with fatal infection: a case report.

BMC Vet Res 2021 Mar 5;17(1):108. Epub 2021 Mar 5.

National Research Center for Veterinary Medicine, No.3 Cuiwei Road, High-Tech District, 471003, Luoyang, Henan, PR China.

Background: Candida is the common conditionally pathogenic fungus that infected human and animal clinically. C. tropicalis had been isolated from the skin and hair of healthy pigs, but with no report of fatal infection in gastrointestinal diseases.

Case Presentation: In a pig farm in Henan Province of China, about 20 % of pregnant and postpartum sows suffered from severe gastrointestinal diseases, with a mortality rate higher than 60 % in the diseased animals. The sows had gastrointestinal symptoms such as blood in stool and vomiting. Necropsy revealed obvious gastric ulcers, gastrointestinal perforation, and intestinal hemorrhage in the gastrointestinal tract, but no lesions in other organs. The microbial species in gastric samples collected from gastric ulcer of the diseased sows then was initially identified as Candida by using routine systems of microscopic examination, culture characteristics on the medium Sabouraud dextrose agar medium. The fungus was further identified as C. tropicalis by species-specific PCR and sequencing. This study revealed an infection of C. tropicalis in sows through gastrointestinal mucosa could cause fatal digestive system disease and septicemia.

Conclusions: For the first time, a strain of C. tropicalis was isolated and identified from the gastric tissue of sows with severe gastrointestinal diseases. PCR and sequencing of ITS-rDNA combined with morphology and histopathological assay were reliable for the identification of Candida clinically.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12917-021-02821-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934564PMC
March 2021

New insights into external layers of cyanobacteria and microalgae based on multiscale analysis of AFM force-distance curves.

Sci Total Environ 2021 Jun 6;774:145680. Epub 2021 Feb 6.

CAS Key Laboratory of Reservoir Water Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; Chongqing School, University of Chinese Academy of Sciences, Chongqing 400714, China. Electronic address:

External layers, the outermost structures around cells, perform essential eco-physiological functions to support cyanobacteria and microalgae in aquatic environments. These layers have been recognized as adaptations to turbulence, a ubiquitous and inherent physical process occurring in the environments of most cyanobacteria and microalgae. However, the underlying biophysical mechanism of these layers is still poorly understood. Force measurements were performed directly on the external layers of eight living cyanobacterial and green algal strains in situ using atomic force microscopy (AFM). We developed a wavelet analysis method based on a multiscale decomposition of derivative force-distance curves to quantify the elastic responses of various external layers upon mechanical deformation. Such analysis has the advantages of detecting singularities and distinguishing the biomechanical contributions of each external layer. The elastic modulus of the same type of external layer follows the same statistical distribution. However, the elastic response among different types of external layers is challenged by our method, indicating the heterogeneity of the mechanical properties of inner and outer layers in multilayer strains. This discrepancy was due to the thickness and texture of each external layer, especially the chemical presence of ribose, hydroxyproline and glutamic acid. This study highlights a new way to elucidate more precise information about external layers and provides a biophysical mechanistic explanation for the functioning of the various external layers to protect cyanobacterial and microalgal cells in a turbulent environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.145680DOI Listing
June 2021

Abietic acid ameliorates psoriasis-like inflammation and modulates gut microbiota in mice.

J Ethnopharmacol 2021 May 17;272:113934. Epub 2021 Feb 17.

State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China; Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210023, China. Electronic address:

Ethnopharmacological Relevance: Abietic acid (AA), an antibacterial terpenoid, was initially isolated from rosin which has been used as a traditional Chinese medicine to treat psoriasis. In our previous works, we found that water-processed rosin (WPR) can alleviate imiquimod (IMQ)-induced psoriasis-like inflammation in mice. However, the efficacy of AA, the main component of WPR, against psoriasis remains unclear.

Materials And Methods: In this study, we confirmed the anti-psoriasis efficacy of AA (40 mg/kg daily for 7 days) in IMQ-induced psoriasis-like inflammation BALB/c mouse model by the psoriasis area severity index (PASI), flow cytometry, ELISA, histopathological and immunohistochemical analysis. Furthermore, we detected the relative abundance of gut microbe using high-throughput 16S rRNA gene sequencing to validate whether AA modulate gut microbe.

Result: Oral administration of AA ameliorates IMQ-induced psoriasis-like skin inflammation through reducing PASI scores, regulating the balance of Th17/Treg cells in the mouse spleen, and downregulating the level of serum cytokines such as TNF-α, IL-17A, TGF-1β, and IL-23. 16S rRNA gene sequencing revealed that the relative abundance of gut bacteria related to inflammation, such as, Anaerotruncus and Christensenella at genus level were decreased, while Kurthia, Citrobacter, and Klebsiella at genus level were increased in AA group mice. Additionally, the correlation analysis illustrated that the key microbiota had a close relationship with the psoriasis-like inflammation related indexes.

Conclusion: AA might exert the anti-psoriasis effect via inhibiting Th17-related immune responses, hinting that it might be a candidate for treating psoriasis. Meanwhile, the alteration of intestinal microbiota by AA treatment is another possible explanation for the amelioration of imiquimod-induced psoriasis-like inflammation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jep.2021.113934DOI Listing
May 2021
-->