Publications by authors named "Yan Wei"

1,837 Publications

  • Page 1 of 1

Pulmonary Embolism at Extreme High Altitude: A Study of Seven Cases.

High Alt Med Biol 2022 May 23. Epub 2022 May 23.

Department of Respiratory Disease, General Hospital of Xinjiang Military Command, Urumqi, China.

Wu, Jialin, Xiaobo Han, Haiwen Ke, Li Wang, Kun Wang, Jianli Zhang, Jun Tang, Wei Yan, Guangjun Wang, and Peng Jiang. Pulmonary embolism at extreme high altitude: A study of seven cases. XX:000-000, 2022. The incidence of venous thromboembolism (VTE) is high in high-altitude (HA) areas. We analyzed cases of pulmonary embolism (PE) in extreme HA areas to explore the epidemiological characteristics and risk factors of PE in these regions. Seven cases of PE occurring in an extreme HA region were prospectively collected at an HA (3,800 m) hospital from May to November 2020. All patients resided 5,000 m above sea level and were diagnosed with PE using computed tomography pulmonary angiography. Seven patients (24 ± 3.6 years old) had symptom onset at a mean altitude of 5,200 ± 200 m, and the duration spent at HA ranged from 8 to 210 days (99.29 ± 77.31 days). Cough, expectoration, chest tightness, fever, shortness of breath, and chest pain were the most common symptoms. Six of the seven patients were initially diagnosed with pulmonary inflammation, and four were diagnosed with high-altitude pulmonary edema using computed tomography or X-ray. Most patients presented with an increased concentration of inflammatory cells and high initial D-dimer levels. In this study, a retrospective analysis of PE case data in extreme HA areas suggested that PE was underdiagnosed owing to misdiagnosis or masking by HA-associated disease.
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http://dx.doi.org/10.1089/ham.2021.0109DOI Listing
May 2022

An efficient strategy for identifying essential proteins based on homology, subcellular location and protein-protein interaction information.

Math Biosci Eng 2022 Apr;19(6):6331-6343

College of Computer Engineering and Applied Mathematics, Changsha University, Changsha, Hunan 410022, China.

High throughput biological experiments are expensive and time consuming. For the past few years, many computational methods based on biological information have been proposed and widely used to understand the biological background. However, the processing of biological information data inevitably produces false positive and false negative data, such as the noise in the Protein-Protein Interaction (PPI) networks and the noise generated by the integration of a variety of biological information. How to solve these noise problems is the key role in essential protein predictions. An Identifying Essential Proteins model based on non-negative Matrix Symmetric tri-Factorization and multiple biological information (IEPMSF) is proposed in this paper, which utilizes only the PPI network proteins common neighbor characters to develop a weighted network, and uses the non-negative matrix symmetric tri-factorization method to find more potential interactions between proteins in the network so as to optimize the weighted network. Then, using the subcellular location and lineal homology information, the starting score of proteins is determined, and the random walk algorithm with restart mode is applied to the optimized network to mark and rank each protein. We tested the suggested forecasting model against current representative approaches using a public database. Experiment shows high efficiency of new method in essential proteins identification. The effectiveness of this method shows that it can dramatically solve the noise problems that existing in the multi-source biological information itself and cased by integrating them.
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http://dx.doi.org/10.3934/mbe.2022296DOI Listing
April 2022

Synergistic Fluoride Adsorption by Composite Adsorbents Synthesized From Different Types of Materials-A Review.

Front Chem 2022 4;10:900660. Epub 2022 May 4.

Xi'an Key Laboratory of Solid Waste Recycling and Resource Recovery, Department of Environmental Science and Engineering, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an, China.

The reduction of fluoride concentrations in water is one of many concerns. Adsorption is the most widely used technology for fluoride removal and the center to development of adsorption technology is the improvement of adsorbents. This review classifies the typical fluoride removal adsorbents into four types: metal oxides/hydroxides, biopolymers, carbon-based, and other adsorbents. The exploitation of new materials and the synthesis of composite materials are two ways of developing new adsorbents. In comparison to the discovery of novel adsorbents for fluoride adsorption, research into the composite synthesis of different types of conventional adsorbents has proliferated in recent years. The traditional adsorbents used the earliest, metal oxides, can act as active centers in a wide range of applications for modifying and compounding with other types of adsorbents. This study emphasizes reviewing the research on fluoride removal by composite adsorbents synthesized from different types of metal-modified materials. Seven factors were compared in terms of material characterization, initial fluoride concentration, adsorbent dose, pH, temperature, reaction time, and maximum adsorption capacity. The modification of composite adsorbents is facile and the synergistic effect of the different types of adsorbents significantly improves fluoride adsorption capacity. Metal composite adsorbents are synthesized by facile coprecipitation, hydrothermal, or impregnation modification methods. The adsorption mechanisms involve electrostatic attraction, ion exchange, complexation, and hydrogen bonding. The fluoride adsorption capacity of composite adsorbents has generally improved, indicating that most modifications are successful and have application prospects. However, to achieve significant breakthroughs in practical applications, numerous issues such as cost, separation/regeneration performance, and safety still need to be considered.
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http://dx.doi.org/10.3389/fchem.2022.900660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114667PMC
May 2022

Perinatal Exposure to Nicotine Alters Sperm RNA Profiles in Rats.

Front Endocrinol (Lausanne) 2022 4;13:893863. Epub 2022 May 4.

The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, United States.

Perinatal exposure to smoking has been associated with childhood asthma, one of the most common pediatric conditions affecting millions of children globally. Of great interest, this disease phenotype appears heritable as it can persist across multiple generations even in the absence of persistent exposure to smoking in subsequent generations. Although the molecular mechanisms underlying childhood asthma induced by perinatal exposure to smoking or nicotine remain elusive, an epigenetic mechanism has been proposed, which is supported by the data from our earlier analyses on germline DNA methylation (5mC) and histone marks (H3 and H4 acetylation). To further investigate the potential epigenetic inheritance of childhood asthma induced by perinatal nicotine exposure, we profiled both large and small RNAs in the sperm of F1 male rats. Our data revealed that perinatal exposure to nicotine leads to alterations in the profiles of sperm-borne RNAs, including mRNAs and small RNAs, and that rosiglitazone, a PPARγ agonist, can attenuate the effect of nicotine and reverse the sperm-borne RNA profiles of F1 male rats to close to placebo control levels.
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http://dx.doi.org/10.3389/fendo.2022.893863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114732PMC
May 2022

Transsphenoidal Surgery of Giant Pituitary Adenoma: Results and Experience of 239 Cases in A Single Center.

Front Endocrinol (Lausanne) 2022 6;13:879702. Epub 2022 May 6.

Department of Neurosurgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: Transsphenoidal surgery (TSS) is first-line treatment for giant pituitary adenomas (PAs). Although PA is a benign neuroendocrine tumor that originates from adenohypophysial cells, the surgical outcomes and prognosis of giant PAs differ significantly due to multiple factors such as tumor morphology, invasion site, pathological characteristics and so on. The aim of this study was to evaluate surgical outcomes of giant PAs in a single-center cohort.

Methods: The clinical features and outcomes of 239 patients with giant PA who underwent sphenoidal surgery at the Second Affiliated Hospital of Zhejiang University School of Medicine from January 2015 to October 2021 were collected from medical records. The basic clinical information (age, gender, function etc.), surgical procedure, imaging features (maximum diameter, invasion characteristics, tumor shape etc.) and histopathological characteristics (pathological results, Ki-67, P53 etc.) were retrospectively reviewed. SPSS 25.0 and Stata 12.0 software were used for statistical analysis.

Results: A total of 239 patients with giant PAs underwent TSS, of which 168 surgeries (70.29%) were endoscopic endonasal transsphenoidal (EETS) and 71 (29.71%) were microscopic transsphenoidal (MTS). The mean preoperative maximum diameter in the cohort was 45.64 mm. Gross-total resection was achieved in 46 patients (19.25%), near-total in 56 (23.43%), subtotal in 68 (28.45%), and partial in 69 (28.87%) patients. The maximum tumor diameter and Knosp grade were the significant factors that limited the extent of the resection of giant PAs. A total of 193 patients (80.75%) experienced surgical complications, and the most common complications were postoperative diabetes insipidus (DI) (91, 38.08%), intracranial infection (36, 15.06%) and cerebrospinal fluid (CSF) leaks (37, 15.48%). In addition, there was a significant difference in the incidence of CSF leaks between the neuroendoscopy group and the microscopic group (P < 0.05).

Conclusion: The management of giant PAs remains a therapeutic challenge due to their large size and postoperative complications. The maximum diameter and Knosp grade of giant PAs significantly limited the extent of resection, which warrants a reasonable surgical plan.
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http://dx.doi.org/10.3389/fendo.2022.879702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120954PMC
May 2022

Uncoupling transcription and translation through miRNA-dependent poly(A) length control in haploid male germ cells.

Development 2022 May 19. Epub 2022 May 19.

BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China.

As one of the post-transcriptional regulatory mechanisms, uncoupling of transcription and translation plays an essential role in development and adulthood physiology. However, it remains elusive how thousands of mRNAs get translationally silenced while stability is maintained for up to hours or even days before translation. In addition to oocytes and neurons, developing spermatids display significant uncoupling of transcription and translation for delayed translation. Therefore, spermiogenesis represents an excellent in vivo model for investigating the mechanism underlying uncoupled transcription and translation. Through full-length poly(A) deep sequencing, we discovered dynamic changes in poly(A) length through deadenylation and re-polyadenylation. Deadenylation appeared to be mediated by microRNAs (miRNAs), and transcripts with shorter poly(A) tails tend to be sequestered into ribonucleoproteins (RNPs) for translational repression and stabilization. In contrast, re-polyadenylation might allow for translocation of the translationally repressed transcripts from RNPs to polysomes. Overall, our data suggest that miRNA-dependent poly(A) length control represents a novel mechanism underlying uncoupled translation and transcription in haploid male germ cells.
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http://dx.doi.org/10.1242/dev.199573DOI Listing
May 2022

Mesoporous Ti O Nanosheets with High Polar Surface Area for Catalyzing Separator to Reduce the Shuttle Effect of Soluble Polysulfides in Lithium-sulfur Batteries.

Chem Asian J 2022 May 19:e202200328. Epub 2022 May 19.

Institute for Sustainable Energy/College of Sciences, Shanghai Unversity, Shanghai, 200444, P. R. China.

In the effort to accelerate adsorption and catalytic conversion of lithium polysulfides (Li-PSs) and suppress the shuttle effect of lithium-sulfur batteries (LSBs), the Ti O nanosheets/carbon material-modified separator is successfully fabricated to reducing soluble Li-PSs' crossover from cathode to anode. The catalyst of mesoporous Ti O nanosheets with O-Ti-O units synthesized at low temperature shows both excellent conductivity and high surface area. The modified separator can serve as a diffusion barrier of Li-PSs and catalyst for converting soluble low-chain sulfides into insoluble ones and then remarkably enhance the sulfur utilization and electrochemical performance of the LSB. This work provides a feasible avenue in both design and synthesis of mesoporous catalyst materials for suppressing the shuttle effect of lithium-sulfur batteries.
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http://dx.doi.org/10.1002/asia.202200328DOI Listing
May 2022

Extent and complexity of RNA processing in honey bee queen and worker caste development.

iScience 2022 May 25;25(5):104301. Epub 2022 Apr 25.

Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, P. R. of China.

The distinct honeybee () worker and queen castes have become a model for the study of genomic mechanisms of phenotypic plasticity. Here we performed a nanopore-based direct RNA sequencing with exceptionally long reads to compare the mRNA transcripts between queen and workers at three points during their larval development. We found thousands of significantly differentially expressed transcript isoforms (DEIs) between queen and worker larvae. These DEIs were formatted by a flexible splicing system. We showed that poly(A) tails participated in this caste differentiation by negatively regulating the expression of DEIs. Hundreds of isoforms uniquely expressed in either queens or workers during their larval development, and isoforms were expressed at different points in queen and worker larval development demonstrating a dynamic relationship between isoform expression and developmental mechanisms. These findings show the full complexity of RNA processing and transcript expression in honey bee phenotypic plasticity.
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http://dx.doi.org/10.1016/j.isci.2022.104301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097701PMC
May 2022

Treatment Strategies in Emergency Endoscopy for Acute Esophageal Variceal Bleeding (CHESS1905): A Nationwide Cohort Study.

Front Med (Lausanne) 2022 27;9:872881. Epub 2022 Apr 27.

Department of Gastroenterology and Endoscopy, The Fifth Affiliated Zhuhai Hospital of Zunyi Medical University, Zhuhai, China.

Background And Aims: Emergency endoscopy is recommended for patients with acute esophageal variceal bleeding (EVB) and their prognosis has improved markedly over past decades due to the increased specialization of endoscopic practice. The study aimed to compare outcomes following emergency endoscopic injection sclerotherapy (EIS) and endoscopic variceal ligation (EVL) in cirrhotic patients with acute EVB.

Methods: Cirrhotic patients with acute EVB who underwent emergency endoscopy were retrospectively enrolled from 2013 to 2020 across 34 university hospitals from 30 cities. The primary outcome was the incidence of 5-day rebleeding after emergency endoscopy. Subgroup analysis was stratified by Child-Pugh class and bleeding history. A 1:1 propensity score matching (PSM) analysis was performed.

Results: A total of 1,017 and 382 patients were included in EIS group and EVL group, respectively. The 5-day rebleeding incidence was similar between EIS group and EVL group (4% vs. 5%, = 0.45). The result remained the same after PSM ( = 1.00). Among Child-Pugh class A, B and C patients, there were no differences in the 5-day rebleeding incidence between the two groups after PSM ( = 0.25, 0.82, and 0.21, respectively). As for the patients with or without bleeding history, the differences between EIS group and EVL group were not significant after PSM ( = 1.00 and 0.26, respectively).

Conclusion: The nationwide cohort study indicates that EIS and EVL are both efficient emergency endoscopic treatment strategies for acute EVB. EIS should not be dismissed as an economical and effective emergency endoscopic treatment strategy of acute EVB. ClincialTrials.gov number NCT04307264.
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http://dx.doi.org/10.3389/fmed.2022.872881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092278PMC
April 2022

Transcriptome-Wide Analysis of RNA N-Methyladenosine Modification in Adriamycin-Resistant Acute Myeloid Leukemia Cells.

Front Genet 2022 28;13:833694. Epub 2022 Apr 28.

Department of Hematology, the Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.

Acute myeloid leukemia (AML) is one of the most aggressive hematopoietic malignancies. Patients still suffer from refractory/relapsed disease after anthracycline-based therapy, which leads to a poor prognosis. N-Methyladenosine (mA) is the most abundant post-transcriptional modification in eukaryotes, the imbalance of which is reported to be associated with various pathological processes, including drug resistance. However, the relationship between mA modification and drug resistance has not been well defined in AML. In this study, we analyzed the sequencing data of HL60 and its Adriamycin-resistant cell line HL60/ADR. We found a total of 40,550 mA-methylated peaks, representing 15,640 genes in HL60, and 38,834 mA-methylated peaks, representing 15,285 genes in HL60/ADR. KEGG pathway analysis showed that pathways were enriched in the FoxO signaling pathway, p53 signaling pathway, and Notch signaling pathway. MeRIP-seq results showed that the fold enrichment of the global mA level in HL60/ADR was higher than that in HL60, and dot blot assay results indicated that the global mA level was elevated in HL60/ADR cells compared with that in HL60 cells. Further analysis revealed that the expression level of METTL3 was elevated in HL60/ADR cells compared with that in HL60 cells. After a combined treatment of STM2457 (an inhibitor of METTL3) and Adriamycin, the proliferation of HL60/ADR was inhibited. Thus, we hypothesized that the abnormality of mA modification played an important role in Adriamycin-resistant AML.
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http://dx.doi.org/10.3389/fgene.2022.833694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100953PMC
April 2022

Unique Pharmacology, Brain Dysfunction, and Therapeutic Advancements for Fentanyl Misuse and Abuse.

Neurosci Bull 2022 May 15. Epub 2022 May 15.

Peking University Sixth Hospital, Peking University Institute of Mental Health, NHC Key Laboratory of Mental Health (Peking University), National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, 100191, China.

Fentanyl is a fully synthetic opioid with analgesic and anesthetic properties. It has become a primary driver of the deadliest opioid crisis in the United States and elsewhere, consequently imposing devastating social, economic, and health burdens worldwide. However, the neural mechanisms that underlie the behavioral effects of fentanyl and its analogs are largely unknown, and approaches to prevent fentanyl abuse and fentanyl-related overdose deaths are scarce. This review presents the abuse potential and unique pharmacology of fentanyl and elucidates its potential mechanisms of action, including neural circuit dysfunction and neuroinflammation. We discuss recent progress in the development of pharmacological interventions, anti-fentanyl vaccines, anti-fentanyl/heroin conjugate vaccines, and monoclonal antibodies to attenuate fentanyl-seeking and prevent fentanyl-induced respiratory depression. However, translational studies and clinical trials are still lacking. Considering the present opioid crisis, the development of effective pharmacological and immunological strategies to prevent fentanyl abuse and overdose are urgently needed.
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http://dx.doi.org/10.1007/s12264-022-00872-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107910PMC
May 2022

Emergence and predominance of a new serotype of Vibrio parahaemolyticus in Huzhou, China.

Int J Infect Dis 2022 May 11. Epub 2022 May 11.

Huzhou Center for Disease Control and Prevention, Huzhou, 313000, China. Electronic address:

Background: During our surveillance period, we found a new type of Vibrio parahaemolyticus (V. parahaemolyticus) with serotype O10:K4 which had increased over the past 2 years in Huzhou, and become the second common serotype following O3:K6.

Methods: Strains were isolated from stool samples of diarrhea patients in the First People's Hospital in Huzhou. The serotypes, virulence-associated genes, Antimicrobial susceptibility and PFGE of these strains were analyzed.

Results: Between January 2017 and December 2021, there were 598 (5.36%) V. parahaemolyticus positive samples of 11166 stool specimens. The V. parahaemolyticus detection rate was high in summer months. O3:K6 was the dominant serotype 2017 to 2020 and a newly serotype O10:K4 was the predominantly serotype in 2021. The majority of isolates tested were resistant to ampicillin (86.8%). We randomly chose the strains with serotype O3:K6 and O10:K4 for PFGE to compare the genetic relationship between these two serotypes. The results showed that the PFGE profiles of V. parahaemolyticus O3:K6 and O10:K4 were genetically similar. The strains showed a tendency to cluster based on their serotype profiles. However, some O3:K6 strains showed 100% similarity with O10:K4 strains.

Conclusions: A new serotype with pandemic potentia of V. parahaemolyticus, O10:K4 was detected in 2020 and became dominant in 2021 in Huzhou.
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http://dx.doi.org/10.1016/j.ijid.2022.05.023DOI Listing
May 2022

Impact of Short-Term Intensive-Type Cognitive Behavioral Therapy Intervention on Internet Addiction among Chinese College Students: A Randomized Controlled Trial.

Int J Environ Res Public Health 2022 Apr 25;19(9). Epub 2022 Apr 25.

Faculty of Health and Sport Sciences, University of Tsukuba, Tsukuba 305-0006, Japan.

The object of this study is to examine the effects of a short-term intensive-type Cognitive Behavioral Therapy (CBT) intervention to prevent internet addiction among Chinese college students. We conducted a randomized controlled trial applying a group counseling intervention program based on CBT. Data included 21 participants in the intervention group and 22 participants in the control group. The results showed that the intervention program reduced college students' internet addiction symptoms and procrastination and improved their sense of coherence. Regarding the sustained effect, internet addiction symptoms decreased and perceived social support from significant others improved in college students. However, the intervention program did not significantly reduce their average daily internet use time and psychological stress. Overall, this study developed a short-term intensive-type intervention program based on CBT theory, which is complementary for Chinese college students with internet addiction.
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http://dx.doi.org/10.3390/ijerph19095212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099546PMC
April 2022

The effect of perceived stress on cognition is mediated by personality and the underlying neural mechanism.

Transl Psychiatry 2022 May 12;12(1):199. Epub 2022 May 12.

Peking University Sixth Hospital, Peking University Institute of Mental Health, NHC Key Laboratory of Mental Health (Peking University), National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Peking University, Beijing, 100191, China.

Perceived stress impairs cognitive function across the adult lifespan, but the extent to which cognition decline is variable across individuals. Individual differences in the stress response are described as personality traits. Substantial individual differences in the magnitude of cognitive impairment that is induced by short-term perceived stress are poorly understood. The present study tested the hypothesis that the relationship between short-term perceived stress and different aspects of cognition is mediated by personality traits. The study included 1066 participants with behavior and neuroimaging data from the Human Connectome Project after excluding individuals with missing variables. In the result, the parallel multiple mediation model demonstrated that the influence of perceived stress on the total and crystalized cognition is mainly mediated by neuroticism (indirect effect = -0.04, p < 0.05) and conscientiousness (indirect effect = 0.05, p < 0.05) in adults. Cortical thickness value (n = 1066) of the right superior frontal gyrus (SFG) showed not only positive correlations with short-term perceived stress and neuroticism, but negative associations with cognition. The chain mediation model found that the right SFG and neuroticism play a small but significant chain mediating effect between stress and total cognition. The strength of the resting-state functional connectivity (n = 968) between the left orbitofrontal cortex versus the left superior medial frontal cortex was positively correlated with crystallized cognition and negatively associated with conscientiousness. These results extend previous findings by the impacts of short-term perceived stress on cognitive function is mediated by neuroticism and the right SFG was the underlying neural mechanism.
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http://dx.doi.org/10.1038/s41398-022-01929-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098451PMC
May 2022

Cynarin suppresses gouty arthritis induced by monosodium urate crystals.

Bioengineered 2022 May;13(5):11782-11793

Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

The study is aimed to determine the effects of cynarin (Cyn) on mice with gouty arthritis (GA) induced by monosodium urate (MSU). We measured swelling in the hind paws of mice using Vernier calipers and ultrasound. The liver, kidney, and hind paws were stained with hematoxylin-eosin, and M1 type macrophages were detected in the hind paws using anti-F4/80 and anti-iNOS antibodies. The mRNA expression of inflammatory factors in bone marrow-derived macrophages (BMDMs) and in the hind paws was detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family pyrin domain containing 3 (NLRP3) inflammasomes and the nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways were analyzed via western blotting. Cyn was detected using Cell Counting Kit-8 (CCK-8). Cyn treatment reduced hind paw swelling and M1 macrophage infiltration, suppressed the mRNA expression of inflammatory factors, and inhibited NLRP3 inflammasome activation , in addition to inhibiting the phosphorylation of IKKa/β, p65, and c-Jun NH 2-terminal kinase (JNK). Furthermore, Cyn exerted anti-inflammatory and anti-swelling effects in mice with GA by regulating the NF-κB and JNK pathways and NLRP3 inflammasomes.
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http://dx.doi.org/10.1080/21655979.2022.2072055DOI Listing
May 2022

Enabling Breeding Selection for Biomass in Slash Pine Using UAV-Based Imaging.

Plant Phenomics 2022 22;2022:9783785. Epub 2022 Apr 22.

Research Institute of Subtropical Forestry, Chinese Academy of Forestry, No. 73, Daqiao Road, Fuyang, Hangzhou, 311400 Zhejiang Province, China.

Traditional methods used to monitor the aboveground biomass (AGB) and belowground biomass (BGB) of slash pine () rely on on-ground measurements, which are time- and cost-consuming and suited only for small spatial scales. In this paper, we successfully applied unmanned aerial vehicle (UAV) integrated with structure from motion (UAV-SfM) data to estimate the tree height, crown area (CA), AGB, and BGB of slash pine for in slash pine breeding plantations sites. The CA of each tree was segmented by using marker-controlled watershed segmentation with a treetop and a set of minimum three meters heights. Moreover, the genetic variation of these traits has been analyzed and employed to estimate heritability ( ). The results showed a promising correlation between UAV and ground truth data with a range of from 0.58 to 0.85 at 70 m flying heights and a moderate estimate of for all traits ranges from 0.13 to 0.47, where site influenced the value of slash pine trees, where in site 1 ranged from 0.13~0.25 lower than that in site 2 (range: 0.38~0.47). Similar genetic gains were obtained with both UAV and ground truth data; thus, breeding selection is still possible. The method described in this paper provides faster, more high-throughput, and more cost-effective UAV-SfM surveys to monitor a larger area of breeding plantations than traditional ground surveys while maintaining data accuracy.
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http://dx.doi.org/10.34133/2022/9783785DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9057123PMC
April 2022

LIMD2 promotes tumor proliferation, invasion, and epithelial-mesenchymal transition in clear cell renal cell carcinoma.

Neoplasma 2022 May 9. Epub 2022 May 9.

Department of Urology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

LIMD2 was found upregulated in various tumors and metastatic samples and associated with a poor prognosis. But the role of LIMD2 in clear cell renal cell carcinoma (ccRCC) remains elusive. The expression of LIMD2 in ccRCC was analyzed using cohort data downloaded from TCGA and ICGC databases. In vitro and in vivo experiments were then conducted to study the biological role of LIMD2 in ccRCC and explore the possible mechanism. The results indicated that LIMD2 was overexpressed and correlated with a poor outcome in ccRCC. LIMD2 promoted the malignancy of ccRCC both in vitro and in vivo. LIMD2 induced epithelial-mesenchymal transition (EMT) via activating the ILK/Akt pathway in ccRCC. In conclusion, LIMD2 is overexpressed and promotes proliferation, invasion, and EMT in ccRCC, which may serve as a potential novel therapeutic target for ccRCC.
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http://dx.doi.org/10.4149/neo_2022_211130N1701DOI Listing
May 2022

Correlation between in vivo microdialysis pharmacokinetics and ex vivo permeation for sinomenine hydrochloride transfersomes with enhanced skin absorption.

Int J Pharm 2022 May 4;621:121789. Epub 2022 May 4.

Shool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, China. Electronic address:

Transdermal drug delivery systems have drawn increasing attention in recent decades. Estimation of the correlation between ex vivo permeation and in vivo absorption (EVIVC) is an indispensable issue in the research and development of transdermal pharmaceutical products. In this paper, sinomenine hydrochloride (SH) transfersomes (SHTs) were prepared with sodium deoxycholate as edge activator, while SH liposomes (SHLs) were prepared as a control preparation. The transdermal permeation characteristics differences between them were explored by an ex vivo skin permeation experiment with Franz diffusion cell and an in vivo skin/blood pharmacokinetic experiment facilitated by double-sited microdialysis sampling technique. The curves of percentage absorbed versus time (absorption curves) under the skin and in the blood were plotted according to the percentages calculated by the deconvolution approach with the application of Wagner-Nelson model, and were correlated with the ex vivo permeation curves to evaluate a level A correlation, while a level C correlation evaluation was conducted based on the in vivo steady-state blood concentration (C) and the ex vivo steady-state transdermal permeation rate. The ex vivo permeation test indicated that the cumulative transdermal permeated amount of SH at 36 h in SHTs was about 1.7 times of that in SHLs. The skin pharmacokinetic data showed that the C and AUC of SHTs were about 8.8 and 8.0 times of those of SHLs, respectively, and the MRT of SHTs was shorter. The blood pharmacokinetic data showed that the C and AUC of SHTs were about 3.7 and 2.9 times of those of SHLs, respectively. The in vivo absorption curves were correlated well with the ex vivo permeation curves. The squares of correlation coefficient (R) for SHTs and SHLs were 0.9153 and 0.9355 respectively in the skin, were 0.8536 and 0.7747 respectively in the blood. As to level C EVIVC, there was no significant difference between the predicted C from ex vivo and the measured Cin vivo. The transfersomes can be employed as effective vehicles to promote the transdermal absorption of SH, and it is feasible to predict the in vivo skin/blood pharmacokinetic properties of SHLs and SHTs based on the ex vivo skin permeation characteristics.
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http://dx.doi.org/10.1016/j.ijpharm.2022.121789DOI Listing
May 2022

Relationships Between Memory Impairments and Hippocampal Structure in Patients With Subcortical Ischemic Vascular Disease.

Front Aging Neurosci 2022 18;14:823535. Epub 2022 Apr 18.

Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.

Background And Purpose: Patients with subcortical ischemic vascular disease (SIVD) suffer from memory disorders that are thought to be associated with the hippocampus. We aimed to explore changes in hippocampal subfields and the relationship between different hippocampal subfield volumes and different types of memory dysfunction in SIVD patients.

Methods: A total of 77 SIVD patients with cognitive impairment (SIVD-CI, = 39) or normal cognition (HC-SIVD, = 38) and 41 matched healthy controls (HCs) were included in this study. Memory function was measured in all subjects, and structural magnetic resonance imaging (MRI) was performed. Then, the hippocampus was segmented and measured by FreeSurfer 6.0 software. One-way ANOVA was used to compare the volume of hippocampal subfields among the three groups while controlling for age, sex, education and intracranial volume (ICV). Then, tests were used to evaluate differences between each pair of groups. Finally, correlations between significantly different hippocampal subfield volumes and memory scores were tested in SIVD patients.

Results: Almost all hippocampal subfields were significantly different among the three groups except for the bilateral hippocampal fissure ( = 0.366, = 0.086, respectively.) and left parasubiculum ( = 0.166). Furthermore, the SIVD-CI patients showed smaller volumes in the right subiculum ( < 0.001), CA1 ( = 0.002), presubiculum ( = 0.002) and molecular layer of the hippocampus ( = 0.017) than the HC-SIVD patients. In addition, right subiculum volumes were positively related to Rey's Auditory Verbal Learning Test (RAVLT) word recognition ( = 0.230, = 0.050), reverse digit span test (R-DST) ( = 0.326, = 0.005) and Rey-Osterrieth Complex Figure Test (ROCF) immediate recall ( = 0.247, = 0.035) scores, right CA1 volumes were positively correlated with RAVLT word recognition ( = 0.261, = 0.026), and right presubiculum volumes showed positive relationships with R-DST ( = 0.254, = 0.030) and ROCF immediate recall ( = 0.242, = 0.039) scores.

Conclusion: SIVD might lead to general reductions in volume in multiple hippocampal subfields. However, SIVD-CI patients showed atrophy in specific subfields, which might be associated with memory deficits.
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http://dx.doi.org/10.3389/fnagi.2022.823535DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062133PMC
April 2022

Collecting and deactivating TGF-β1 hydrogel for anti-scarring therapy in post-glaucoma filtration surgery.

Mater Today Bio 2022 Mar 18;14:100260. Epub 2022 Apr 18.

School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150080, Heilongjiang Province, People's Republic of China.

Scar formation can lead to glaucoma filtration surgery (GFS) failure, wherein transforming growth factor (TGF)-β is the core regulator. To reducing scar formation, this paper presents our study on the design of hydrogels to deactivate TGF-β1. We hypothesized that excess TGF-β1 can be removed from aqueous humor through the addition of oxidized hyaluronic acid (O-HA) hydrogels that are seeded with decorin (O-HA ​+ ​D). Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were performed to demonstrate the adsorption properties of O-HA ​+ ​D hydrogel, thus reducing the TGF-β1 concentration in aqueous humor. In the light that collagen contraction in human Tenon's capsule fibroblasts (HTFs) and the angiogenesis of human umbilical vein endothelial cells (HUVECs) can be activated by TGF-β1 and β2, we performed the quantitative analysis of polymerase chain reaction to determine the effect of O-HA ​+ ​D on the type I collagen, fibronectin, and angiogenesis. Our results illustrate that O-HA ​+ ​D can inhibit the increase of α-SMA expression in HTF induced by TGF-β1 and that O-HA ​+ ​D can inhibit the production of collagen I and fibronectin in HTF treated with TGF-β1. Furthermore, we performed in vivo studies by employing a rabbit model, where rabbits were treated with hydrogels post GFS. Our results demonstrate that, as compared with other groups, the rabbits treated with O-HA ​+ ​D had the greatest reduction in inflammatory cells with reduced level of collagen in wounds. Taken together, the present study paves the way toward the treatment of post-glaucoma fibrosis following surgery.
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http://dx.doi.org/10.1016/j.mtbio.2022.100260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061788PMC
March 2022

Mitigation of microbial corrosion by Cu addition to X65 pipeline steel by Pseudomonas aeruginosa MCCC 1A00099.

Arch Microbiol 2022 May 6;204(6):299. Epub 2022 May 6.

Institute of Metal Research, Chinese Academy of Sciences, Shenyang, 110016, China.

Microbiologically influenced corrosion (MIC) is becoming a knotty problem for transmission pipelines. Developing MIC mitigation strategies for pipelines is increasingly urgent. In this study, MIC resistance against Pseudomonas aeruginosa of the X65 pipeline steels with (X65Cu) and without (X65) Cu addition was comparatively studied by electrochemical measurements and surface observation. Experimental results demonstrated that the corrosion rate of X65Cu steel was lower than that of X65 steel no matter in sterile or bacteria-containing media. Cu addition is helpful to the formation of the rust layer in the sterile medium. Surface observation showed that X65Cu steel exhibited a better MIC resistance against P. aeruginosa than that of X65 steel. Cu ions released from the X65Cu steel could effectively kill the P. aeruginosa attached on the steel surface, thus evidently decreased the pit depth and diameter.
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http://dx.doi.org/10.1007/s00203-022-02926-6DOI Listing
May 2022

DDX04+ Stem Cells in the Ovaries of Postmenopausal Women: Existence and Differentiation Potential.

Stem Cells 2022 03;40(1):88-101

Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.

Ovarian aging is a pacemaker with multiple organ dysfunction. Recently, stem cells with the ability to generate new oocytes have been identified, which provides the possibility of stem cell therapy for ovarian aging. Several studies have revealed the existence of stem cells in the human postmenopausal ovary. In this study, we describe a new method using magnetic-activated cell sorting combined with differential adhesion to isolate DDX4+ stem cells from ovaries of postmenopausal women and show that the cells exhibit similar gene expression profiles and growth characteristics with primitive germ cells. Furthermore, the DDX4+ stem cells could enter the meiosis stage and differentiation into oocytes. The RNA-seq data of the differentiated oocytes shows that mitochondrial metabolism may play an important role in the oogenesis process of the DDX4+ stem cells. Through using the human ovarian cortical fragments transplantation model, we indicated that the GFP-DDX4+ stem cells differentiated into some GFP positive oocyte-like structure in vivo. Our study provided a new method for the isolation of DDX4+ stem cells from the ovaries of postmenopausal women and confirmed the ability of these stem cells to differentiate into oocytes.
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http://dx.doi.org/10.1093/stmcls/sxab002DOI Listing
March 2022

Engineering DNA-Guided Hydroxyapatite Bulk Materials with High Stiffness and Outstanding Antimicrobial Ability for Dental Inlay Applications.

Adv Mater 2022 Apr 30:e2202180. Epub 2022 Apr 30.

J. Deng, Prof. Y. Wei, Department of Geriatric Dentistry, Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing, 100081, China.

Programmable base pair interactions at the nanoscale make DNA an attractive scaffold for forming hydroxyapatite (HAP) nanostructures. However, engineering macroscale HAP mineralization guided by DNA molecules remains challenging. To overcome this issue, we developed a facile strategy for the fabrication of ultra-stiff DNA-HAP bulk composites. The electrostatic complexation of DNA and a surfactant with a quaternary ammonium salt group enabled the formation of long-range ordered scaffolds using electrospinning. The growth of one- and two-dimensional HAP minerals were thus realized by this DNA template at a macroscale. Remarkably, the as-prepared DNA-HAP composites exhibited an ultra-high Young's modulus of approximately 25 GPa, which is comparable to natural HAP and superior to most artificial mineralized composites. Furthermore, a new type of dental inlay with outstanding antibacterial properties was developed using the stiff DNA-HAP. The encapsulated quaternary ammonium group within the dense HAP endowed the composite with long-lasting and local antibacterial activity. Therefore, this new type of super-stiff biomaterial holds great potential for oral prosthetic applications. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202202180DOI Listing
April 2022

Preparation of Templated Materials and Their Application to Typical Pollutants in Wastewater: A Review.

Front Chem 2022 5;10:882876. Epub 2022 Apr 5.

Xi'an Key Laboratory of Solid Waste Recycling and Resource Recovery, Department of Environmental Science and Engineering, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an, China.

As the pollution and destruction of global water resources become more and more severe, the treatment of wastewater has attracted significant attention. The template method is a synthetic method in which the template is the main configuration to control, influence, and modify the morphology as well as control the dimensions of the material, thus achieving the properties that determine the material. It is simple, highly reproducible, and predictable, and more importantly, it can effectively control the pore structure, size, and morphology of the material, providing a novel platform for the preparation of adsorbent materials with excellent adsorption properties. This review focuses on the classification of the templates according to their properties and spatial domain-limiting capabilities, reviews the types of hard and soft template materials and their synthetic routes, and further discusses the modulation of the morphological structure of the materials by the introduction of templates. In addition, the application and adsorption mechanisms of heavy metal ions and dyes are reviewed based on the regulatory behavior of the template method.
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http://dx.doi.org/10.3389/fchem.2022.882876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037039PMC
April 2022

Colorizing Grayscale CT images of human lungs using deep learning methods.

Multimed Tools Appl 2022 Apr 22:1-15. Epub 2022 Apr 22.

Auckland University of Technology, Auckland, 1010 New Zealand.

Image colorization refers to computer-aided rendering technology which transfers colors from a reference color image to grayscale images or video frames. Deep learning elevated notably in the field of image colorization in the past years. In this paper, we formulate image colorization methods relying on exemplar colorization and automatic colorization, respectively. For hybrid colorization, we select appropriate reference images to colorize the grayscale CT images. The colours of meat resemble those of human lungs, so the images of fresh pork, lamb, beef, and even rotten meat are collected as our dataset for model training. Three sets of training data consisting of meat images are analysed to extract the pixelar features for colorizing lung CT images by using an automatic approach. Pertaining to the results, we consider numerous methods (i.e., loss functions, visual analysis, PSNR, and SSIM) to evaluate the proposed deep learning models. Moreover, compared with other methods of colorizing lung CT images, the results of rendering the images by using deep learning methods are significantly genuine and promising. The metrics for measuring image similarity such as SSIM and PSNR have satisfactory performance, up to 0.55 and 28.0, respectively. Additionally, the methods may provide novel ideas for rendering grayscale X-ray images in airports, ferries, and railway stations.
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http://dx.doi.org/10.1007/s11042-022-13062-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027015PMC
April 2022

Cancer-cell-secreted miR-122 suppresses O-GlcNAcylation to promote skeletal muscle proteolysis.

Nat Cell Biol 2022 May 25;24(5):793-804. Epub 2022 Apr 25.

Department of Pathology, University of California, San Diego, La Jolla, CA, USA.

A decline in skeletal muscle mass and low muscular strength are prognostic factors in advanced human cancers. Here we found that breast cancer suppressed O-linked N-acetylglucosamine (O-GlcNAc) protein modification in muscle through extracellular-vesicle-encapsulated miR-122, which targets O-GlcNAc transferase (OGT). Mechanistically, O-GlcNAcylation of ryanodine receptor 1 (RYR1) competed with NEK10-mediated phosphorylation and increased K48-linked ubiquitination and proteasomal degradation; the miR-122-mediated decrease in OGT resulted in increased RYR1 abundance. We further found that muscular protein O-GlcNAcylation was regulated by hypoxia and lactate through HIF1A-dependent OGT promoter activation and was elevated after exercise. Suppressed O-GlcNAcylation in the setting of cancer, through increasing RYR1, led to higher cytosolic Ca and calpain protease activation, which triggered cleavage of desmin filaments and myofibrillar destruction. This was associated with reduced skeletal muscle mass and contractility in tumour-bearing mice. Our findings link O-GlcNAcylation to muscular protein homoeostasis and contractility and reveal a mechanism of cancer-associated muscle dysregulation.
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http://dx.doi.org/10.1038/s41556-022-00893-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107513PMC
May 2022

Percutaneous coronary intervention in insulin-treated diabetic patients: A meta-analysis.

Ann Noninvasive Electrocardiol 2022 Apr 25:e12953. Epub 2022 Apr 25.

Department of General Practice, Jinshan Hospital of Fudan University, Shanghai, China.

Background: This meta-analysis of randomized controlled trials (RCTs) compared long-term adverse clinical outcomes of percutaneous coronary intervention (PCI) in insulin-treated diabetes mellitus (ITDM) and non-ITDM patients.

Methods: This is a meta-analysis study. The PubMed and Embase databases were searched for articles on long-term adverse clinical outcomes of PCI in ITDM and non-ITDM patients. The risk ratios (RR) and 95% confidence intervals (CI) were calculated.

Results: A total of 11 related RCTs involving 8853 DM patients were included. Compared with non-ITDM patients, ITDM patients had significantly higher all-cause mortality (ACM) (RR = 1.52, 95% CI: 1.25-1.85, p  = .689, I  = 0%), major adverse cardiac and cerebrovascular events (MACCE) (RR = 1.35, 95% CI: 1.18-1.55, p  = .57, I  = 0%), myocardial infarction (MI) (RR = 1.41, 95% CI: 1.16-1.72, p  = .962, I  = 0%), and stent thrombosis (ST) (RR = 1.75, 95% CI: 1.23-2.48, p  = .159, I  = 32.4%). No significant difference was found in the target lesion revascularization (TLR) and target vessel revascularization (TVR) between the ITDM and non-ITDM groups.

Conclusions: The results showed that ITDM patients had significantly higher ACM, MACCE, MI, and ST, compared with non-ITDM patients.
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http://dx.doi.org/10.1111/anec.12953DOI Listing
April 2022

Exploration of Reduced Mitochondrial Content-Associated Gene Signature and Immunocyte Infiltration in Colon Adenocarcinoma by an Integrated Bioinformatic Analysis.

Front Genet 2022 8;13:832331. Epub 2022 Apr 8.

Department of Radiation and Medical Oncology, Zhongnan Hospital Wuhan University, Wuhan, China.

Mitochondrial dysfunction refers to cancer immune evasion. A novel 7-gene prognostic signature related to the mitochondrial DNA copy number was utilized to evaluate the immunocyte infiltration in colon cancer according to the risk scores and to predict the survival for colon cancer. We performed an integrated bioinformatic analysis to analyze transcriptome profiling of the EB-treated mitochondrial DNA-defected NCM460 cell line with differentially expressed genes between tumor and normal tissues of COAD in TCGA. The LASSO analysis was utilized to establish a prognostic signature. ESTIMATE and CIBERSORT validated the differences of immunocyte infiltration between colon cancer patients with high- and low-risk scores. Our study identified a 7-gene prognostic signature (, , , , , , and ). Patients with colon cancer were split into the high- and low-risk group by the risk scores in TCGA (training cohort: HR = 2.50 < 0.0001) and GSE39582 (validation cohort: HR = 1.43 < 0.05). ESTIMATE and CIBERSORT revealed diverseness of immune infiltration in the two groups, especially downregulated T-cell infiltration in the patients with high-risk scores. Finally, we validated the colon patients with a low expression of the mitochondrial number biomarker TFAM had less CD3 and CD8 T-cell infiltration in clinical specimens. An mtDNA copy number-related 7-gene prognostic signature was investigated and evaluated, which may help to predict the prognosis of colon cancer patients and to guide clinical immunotherapy immunocyte infiltration evaluation.
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http://dx.doi.org/10.3389/fgene.2022.832331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9024084PMC
April 2022

Extracellular Vesicles, New Players in Sepsis and Acute Respiratory Distress Syndrome.

Front Cell Infect Microbiol 2022 7;12:853840. Epub 2022 Apr 7.

Department of Critical Care Medicine, Renmin Hospital of Wuhan University, Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China.

Sepsis refers to a complex syndrome associated with physiological, pathological, and biochemical abnormalities resulted from infection. Sepsis is the major cause of acute respiratory distress syndrome (ARDS). Extracellular vesicles (EVs) are serving as new messengers to mediate cell-cell communication . Non-coding RNAs, proteins and metabolites encapsulated by EVs could result in either pro-inflammatory or anti-inflammatory effects in the recipient cells. Pathogens or host cells derived EVs play an important role in pathogens infection during the occurrence and development of sepsis and ARDS. Additionally, we summarize the potential application for EVs in diagnosis, prevention and treatment for sepsis and ARDS.
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http://dx.doi.org/10.3389/fcimb.2022.853840DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021632PMC
April 2022

Vergence Formula for Estimating the Refractive Status of Aphakic Eyes in Pediatric Patients.

Front Med (Lausanne) 2022 8;9:861745. Epub 2022 Apr 8.

Department of Ophthalmology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Clinical Relevance: A vergence formula may provide a simple and reliable calculation of the refractive status of aphakic eyes.

Background: Measuring the refractive error of pediatric eyes with aphakia is difficult. This study investigated the accuracy and applicability of a vergence formula for estimating the refractive status of such eyes.

Methods: A retrospective review of the medical records, created between January 2016 and December 2018, of pediatric patients with aphakia was conducted. A vergence formula, based on axial length, was used to calculate the refractive status of the aphakic eyes. The refractive values determined using retinoscopy, an automatic refractometer, and the vergence formula were compared.

Results: A total of 72 eyes (47 patients) were analyzed. The spherical equivalents of the refractive errors (mean ± standard deviation) of the eyes were determined using retinoscopy (13.01 ± 3.27 D), automatic refractometry (12.90 ± 3.23 D), and the vergence formula (12.70 ± 3.4 D). The correlation coefficient between retinoscopy values determined using retinoscopy and the vergence formula, automatic refractometry and the vergence formula, and retinoscopy and automatic refractometry were 0.968, 0.987, and 0.979, respectively. The Bland-Altman consistency analysis revealed that the mean differences in the spherical equivalent values between retinoscopy and automatic refractometry, retinoscopy and the vergence formula, and automatic refractometry and the vergence formula were 0.11 D, 0.31 D, and 0.21 D, respectively, with 95% limits of agreement of-1.20 to 1.41 D,-1.37 to 2.00 D, and-0.90 to 1.31 D, respectively.

Conclusion: The vergence formula was effective for evaluating the refractive status of aphakic eyes in pediatric patients.
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http://dx.doi.org/10.3389/fmed.2022.861745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032793PMC
April 2022
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