Publications by authors named "Yan Wang"

10,839 Publications

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Recent advance in the fabrication of carbon nanofiber-based composite materials for wearable devices.

Nanotechnology 2021 Jul 29. Epub 2021 Jul 29.

Qingdao University, Ningxia Road 308, qingdao University, Normal college, Qingdao, Qingdao, 266071, CHINA.

Carbon nanofibers (CNFs) exhibit the advantages of high mechanical strength, good conductivity, easy production, and low cost, which have shown wide applications in the fields of materials science, nanotechnology, biomedicine, tissue engineering, sensors, wearable electronics, and other aspects. To promote the applications of CNF-based nanomaterials in wearable devices, the flexibility, electronic conductivity, thickness, weight, and bio-safety of CNF-based films/membranes are crucial. In this review, we present recent advances in the fabrication of CNF-based composite nanomaterials for flexible wearable devices. For this aim, firstly we introduce the synthesis and functionalization of CNFs, which promote the optimization of physical, chemical, and biological properties of CNFs. Then, the fabrication of two-dimensional and three-dimensional CNF-based materials are demonstrated. In addition, enhanced electric, mechanical, optical, magnetic, and biological properties of CNFs through the hybridization with other functional nanomaterials by synergistic effects are presented and discussed. Finally, wearable applications of CNF-based materials for flexible batteries, supercapacitors, strain/piezoresistive sensors, bio-signal detectors, and electromagnetic interference shielding devices are introduced and discussed in detail. We believe that this work will be beneficial for readers and researchers to understand both structural and functional tailoring of CNFs, and to design and fabricate novel CNF-based flexible and wearable devices for advanced applications.
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http://dx.doi.org/10.1088/1361-6528/ac18d5DOI Listing
July 2021

Incidence rates of influenza illness during pregnancy in Suzhou, China, 2015-2018.

Influenza Other Respir Viruses 2021 Jul 29. Epub 2021 Jul 29.

Suzhou Center for Disease Control and Prevention, Suzhou, China.

Background: Data on influenza incidence during pregnancy in China are limited.

Methods: From October 2015 to September 2018, we conducted active surveillance for acute respiratory illness (ARI) among women during pregnancy. Nurses conducted twice weekly phone and text message follow-up upon enrollment until delivery to identify new episodes of ARI. Nasal and throat swabs were collected ≤10 days from illness onset to detect influenza.

Results: In total, we enrolled 18 724 pregnant women median aged 28 years old, 37% in first trimester, 48% in second trimester, and 15% in third trimester, with seven self-reported influenza vaccination during pregnancy. In the 18-week epidemic period during October 2015 to September 2016, influenza incidence was 0.7/100 person-months (95% CI: 0.5-0.9). In the cumulative 29-week-long epidemic during October 2016 to September 2017, influenza incidence was 1.0/100 person-months (95% CI: 0.8-1.2). In the 11-week epidemic period during October 2017 to September 2018, influenza incidence was 2.1/100 person-months (95% CI: 1.9-2.4). Influenza incidence was similar by trimester. More than half of the total influenza illnesses had no elevated temperature and cough. Most influenza-associated ARIs were mild, and <5.1% required hospitalization.

Conclusions: Influenza illness in all trimesters of pregnancy was common. These data may help inform decisions regarding the use of influenza vaccine to prevent influenza during pregnancy.
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http://dx.doi.org/10.1111/irv.12888DOI Listing
July 2021

Viral Metagenomics Reveals Diverse Viruses in the Feces Samples of Raccoon Dogs.

Front Vet Sci 2021 12;8:693564. Epub 2021 Jul 12.

School of Medicine, Jiangsu University, Zhenjiang, China.

Raccoon dogs as an ancient species of are the host of many viruses, including rabies virus, canine distemper virus, severe acute respiratory syndrome coronavirus, and so on. With the development of raccoon dog breeding in recent years, some viruses which infected poultry or pigs were also detected from raccoon dogs. At present, the fecal virome of raccoon dogs has been rarely studied. Using an unbiased viral metagenomic approach, we investigated the fecal virome in raccoon dogs collected from one farm of Jilin Province, China. Many DNA or RNA viruses identified in those fecal samples were mainly from seven families, including , and . This study increased our understanding of the fecal virome in raccoon dog and provided valuable information for the monitoring, prevention, and treatment of viral diseases of these animals.
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http://dx.doi.org/10.3389/fvets.2021.693564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311183PMC
July 2021

Case Report: Successful Management of a Refractory Plasmablastic Lymphoma Patient With Tislelizumab and Lenalidomide.

Front Immunol 2021 12;12:702593. Epub 2021 Jul 12.

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Plasmablastic lymphoma (PBL) is a rare and aggressive hematological malignancy. PBL commonly occurs in immune incompetent patients, such as those with human immunodeficiency virus (HIV), post-transplant status, or immunosenescence. Given its rarity, there is no specific standard treatment for PBL. However, small case series have shown that intensive chemotherapies combined with anti-myeloma agents such as bortezomib and lenalidomide were effective in treating PBL. Unfortunately, some fragile patients could not tolerate intensive chemotherapeutic regimens, especially the elderly patients. Here we presented a 76-year-old female PBL patient refractory to miniCHOP regimen combined with bortezomib but achieved complete remission when treated with tislelizumab combined with lenalidomide, indicating that immune therapy may be a potential treatment for PBL. To our knowledge, this is the first chemoresistant PBL patient that has been successfully treated with checkpoint inhibitor plus lenalidomide, thus providing new insight towards PBL management.
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http://dx.doi.org/10.3389/fimmu.2021.702593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312258PMC
July 2021

Influence of angiotensin converting enzyme inhibitors/angiotensin receptor blockers on the risk of all-cause mortality and other clinical outcomes in patients with confirmed COVID-19: A systemic review and meta-analysis.

J Clin Hypertens (Greenwich) 2021 Jul 28. Epub 2021 Jul 28.

Department of Cardiology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.

Since the COVID-19 pandemic, physicians concerned about the potential adverse effects of angiotensin converting enzyme inhibitors (ACEIs)/angiotensin receptor blockers (ARBs). To explore the relationship between ACEIs/ARBs and the risk of mortality and other clinical outcomes in COVID-19 patients, the authors conducted a systemic review and meta-analysis. An electronic search was performed from inception to November 12, 2020 in PubMed, Medline, EMBASE, ClinicalTrials, TRIP, the Cochrane Library, CNKI, Wanfang, and CBM database. Risk of bias was assessed using the Risk Of Bias In Non-randomized Studies of Interventions tool. The primary outcome was in-hospital all-cause mortality. Secondary outcomes included all-cause mortality measured at 30-day or longer term, mechanical ventilation, length of hospital stay, readmission, and cardiac adverse events. A total of 28 studies with 73 465 patients was included. Twenty-two studies with 19 871 patients reported the incidence of all-cause mortality. Results showed no association between using ACEIs/ARBs and risk of mortality crude odds ratio (OR) of 1.02, 95% CI 0.71-1.46, p = .90, I = 88%, adjusted OR in 6260 patients of 0.96, 95% CI 0.77-1.18, p = .68, I = 0%. While six studies with 10 030 patients reported a lower risk of mortality in ACEIs/ARBs group hazard ratio (HR) of 0.53, 95% CI 0.34-0.84, p = .007, I = 68%. Similar association (for HR) was found in hypertension subgroup. There was no significant association for the secondary outcomes. Based on the available data, we concluded that ACEIs/ARBs is not associated with the risk of in-hospital all-cause mortality in COVID-19 patients, but may be associated with a decreased risk of 30-day all-cause mortality. Patients with hypertension may benefit from using ACEIs/ARBs.
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http://dx.doi.org/10.1111/jch.14329DOI Listing
July 2021

Low-power consumption light-stimulated synaptic transistors based on natural carotene and organic semiconductors.

Chem Commun (Camb) 2021 Jul 28. Epub 2021 Jul 28.

Interdisciplinary Materials Research Center, School of Materials Science and Engineering, Frontiers Science Center for Intelligent Autonomous Systems, Tongji University, Shanghai, 201804, P. R. China. and Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital Affiliated to Tongji University, Tongji University, Shanghai, 200434, P. R. China.

Developing synaptic devices with environment-friendly materials is a promising research direction. Here, light-stimulated synaptic transistors based on natural carotene and organic semiconductors were developed. Several important functions similar to biological synapses were realized and an ultra-low power consumption of 3.4 × 10-18 J was achieved.
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http://dx.doi.org/10.1039/d1cc03060dDOI Listing
July 2021

Deep Supervised Domain Adaptation for Pneumonia Diagnosis from Chest X-ray Images.

IEEE J Biomed Health Inform 2021 Jul 27;PP. Epub 2021 Jul 27.

Pneumonia is one of the most common treatable causes of death, and early diagnosis allows for early intervention. Automated diagnosis of pneumonia can therefore improve outcomes. However, it is challenging to develop high performance deep learning models due to the lack of well-annotated data for training. This paper proposes a novel method, called Deep Supervised Domain Adaptation (DSDA), to automatically diagnose pneumonia from chest X-ray images. Specifically, we propose to transfer the knowledge from a publicly available large-scale source dataset (ChestX-ray14) to a well-annotated but small-scale target dataset (the TTSH dataset). DSDA aligns the distributions of the source domain and the target domain according to the underlying semantics of the training samples. It includes two task-specific sub-networks for the source domain and the target domain, respectively. These two sub-networks share the feature extraction layers and are trained in an end-to-end manner. Unlike most existing domain adaptation approaches that perform the same tasks in the source domain and the target domain, we attempt to transfer the knowledge from a multi-label classification task in the source domain to a binary classification task in the target domain. To evaluate the effectiveness of our method, we compare it with several existing peer methods. The experimental results show that our method can achieve promising performance for automated pneumonia diagnosis.
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http://dx.doi.org/10.1109/JBHI.2021.3100119DOI Listing
July 2021

The level of carbon dioxide is the determinant of successful non-invasive ventilation pressure titration in patients with non-hypercapnic primary central sleep apnea: a case report.

J Clin Sleep Med 2021 Jul 27. Epub 2021 Jul 27.

Respiratory and Critical Care Medicine Department of Tianjin Medical University General Hospital, Tianjin, China.

Primary central sleep apnea (CSA) is classified as non-hypercapnic CSA. High loop gain, lower carbon dioxide (CO) reserves, and other reasons can lead to hypocapnia in patients who develop intermittent hyperventilation during sleep. Therefore, it is necessary to monitor nocturnal CO level for these patients. We report a female patient diagnosed with non-hypercapnic primary CSA who complained of snoring, apnea, and excessive daytime sleepiness. With the monitoring of transcutaneous partial pressure of CO (PtcCO), manual non-invasive ventilation (NIV) pressure titration was performed with continuous positive airway pressure (CPAP), bilevel positive airway pressure in a spontaneous-timed mode (BPAP-ST), and adaptive servo-ventilation (ASV) mode for three nights, respectively. Only ASV mode could stabilize the PtcCO above the apneic threshold (AT, approximately 40 mmHg) with successfully eliminating central apnea events. It is concluded that the level of CO is the determinant of successful NIV pressure titration in patients with non-hypercapnic CSA.
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http://dx.doi.org/10.5664/jcsm.9568DOI Listing
July 2021

Effects of precipitation change and nitrogen addition on soil net N mineralization in a saline-alkaline grassland of Northern Shanxi Province, China.

Ying Yong Sheng Tai Xue Bao 2021 Jul;32(7):2389-2396

College of Agriculture, Henan Univer-sity of Science and Technology, Luoyang 471023, Henan, China.

To explore the responses of soil net nitrogen (N) mineralization rate to precipitation varia-tion and nitrogen deposition in salinized grassland, we set precipitation manipulation and nitrogen addition experiments in the typical agro-pastoral ecotone saline-alkaline grassland of Northern Shanxi Province, China. The soil net N mineralization rate was determined by top-cover buried PVC cylinder from May to September in 2019. The results showed that there were seasonal dynamics in soil net N mineralization rate. Soil net N mineralization rate was not affected by increase/decrease precipitation (±50%), nitrogen addition (10 g·m·a) or the combination of nitrogen addition and increase 50% precipitation treatments. The combination of nitrogen addition and 50% reduction of precipitation significantly improved soil net nitrification rate and net N mine-ralization rate by 10.8 and 8.6 times, respectively. Soil net nitrogen mineralization rate was positively related to soil water content, and negatively related to soil pH. The effects of nitrogen addition on soil nitrogen mineralization rate were dependent on precipitation conditions. Soil water content and pH were important factors regulating soil net nitrogen mineralization rate in the saline-alkaline grassland of Northern Shanxi Province. Therefore, to roundly assess the response model of soil N mine-ralization process to global change, it is necessary to consider the interaction of precipitation changes and nitrogen addition, and the soil physical and chemical properties of salinized grassland.
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http://dx.doi.org/10.13287/j.1001-9332.202107.009DOI Listing
July 2021

Efficacy and safety of tenofovir disoproxil fumarate and tenofovir alafenamide fumarate in preventing HBV vertical transmission of high maternal viral load.

Hepatol Int 2021 Jul 26. Epub 2021 Jul 26.

Science and Education Department, The Sixth People's Hospital of Shenyang, Shenyang, 110006, Liaoning, People's Republic of China.

Background: Hepatitis B virus (HBV) infection is a significant global health problem and > 42-52% of patients are infected during perinatal period. Tenofovir alafenamide fumarate (TAF) and tenofovir disoproxil fumarate (TDF) have been widely recognized as the main compounds used for antiviral treatment of hepatitis B. The present study evaluated the efficacy and safety of TAF in reducing HBV vertical transmission.

Methods: A total of 72 pregnant women, who met the inclusion criteria, were randomly divided into the TDF (300 mg/day, n = 36) and TAF (25 mg/day, n = 36) groups. Clinical and laboratory data were analyzed and compared between the two groups.

Results: No significant differences in alanine aminotransferase, total bilirubin, blood creatinine and blood urea nitrogen levels were noted between the two groups after treatment. The serum HBV DNA viral load and hepatitis B e antigen (HBeAg) levels of the two groups were significantly decreased following treatment, whereas the difference between the two groups was not statistically significant. The levels of urine retinol-binding protein and β2-microglobulin had no significant change after TAF treatment (p > 0.05), but increased significantly after TDF treatment (p < 0.05). All drug concentrations were undetectable in umbilical cord blood (UCB) and breast milk samples of the TAF group, while the drug concentration of UCB and breast milk samples in the TDF group was 2.98 ± 1.44 and 19.16 ± 15.26 ng/ml, respectively. All infants were tested negative for serum hepatitis B surface antigen, HBV DNA, and HBeAg.

Conclusions: Both TAF and TDF effectively block the mother-to-child transmission of hepatitis B. TAF was superior to TDF with regard to renal safety and breastfeeding.
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http://dx.doi.org/10.1007/s12072-021-10235-1DOI Listing
July 2021

Assessment of EGFP-Q74 degradation for the measurement of autophagic flux.

Methods Cell Biol 2021 27;165:31-38. Epub 2021 Apr 27.

Centre de Recherche des Cordeliers, Équipe 11 Labellisée par la Ligue Contre le Cancer, Université de Paris, Sorbonne Université, Inserm U1138, Institut Universitaire de France, Paris, France; Metabolomics and Cell Biology Platforms, Gustave Roussy Cancer Campus, Villejuif, France. Electronic address:

Autophagy plays a major role in physiological and pathological processes. The quantitation of the abundance of autophagy-specific substrates constitutes an efficient strategy for assessing autophagic activity. Here, we provide a detailed protocol for quantifying the decay of a fusion protein composed by enhanced green fluorescent protein (EGFP) and glutamine repeats (Q74) using regular or high-throughput fluorescence microscopy. This method provides a direct measurement of autophagic flux in a Huntington's disease model.
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http://dx.doi.org/10.1016/bs.mcb.2020.10.018DOI Listing
April 2021

Discovery of Cyclic Peptidomimetic Ligands Targeting the Extracellular Domain of EGFR.

J Med Chem 2021 Jul 23. Epub 2021 Jul 23.

Department of Chemistry, University of South Florida, 4202 E. Fowler Avenue, Tampa, Florida 33620, United States.

It is very promising to target the extracellular domain of epidermal growth factor receptor (EGFR) for developing novel and selective anticancer therapies. Herein, we report the discovery of a novel small molecule, , from a one-bead-two-compound (OBTC) cyclic γ-AApeptide library. The molecule was found to bind tightly to the extracellular domain of EGFR. Intriguingly, this molecule could also effectively antagonize EGF-stimulated EGFR phosphorylation and downstream signal transduction. Furthermore, together with its remarkable resistance to proteolytic degradation, was shown to effectively inhibit cell proliferation and migration in vitro and suppresses the growth of tumor in the A549 xenograft model in vivo, highlighting its potential therapeutic application for cancer treatment.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00607DOI Listing
July 2021

Focus on Autoimmune Myocarditis in Graves' Disease: A Case-Based Review.

Front Cardiovasc Med 2021 7;8:678645. Epub 2021 Jul 7.

Division of Cardiology, Department of Internal Medicine, Tongji Medical College, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.

The manifestations of hyperthyroidism-related myocardial damage are multitudinous, including arrhythmia, dilated cardiomyopathy, valvular diseases, and even cardiogenic shock. Acute myocarditis induced by thyrotoxicosis had been reported in a few studies. However, attention on its prevalence and underlying mechanisms is sorely lacking. Its long-term harm is often ignored, and it may eventually develop into dilated cardiomyopathy and heart failure. We report a case of Graves' disease with a progressive elevation of hypersensitive cardiac troponin-I at several days after discontinuation of the patient's anti-thyroid drugs. Cardiac magnetic resonance imaging (CMRI) showed inflammatory edema of some cardiomyocytes (stranded enhanced signals under T2 mapping), myocardial necrosis (scattered enhanced signals under T1 late gadolinium enhancement) in the medial and inferior epicardial wall, with a decreased left ventricular systolic function (48%), which implied a possibility of acute myocarditis induced by thyrotoxicosis. The patient was then given a transient glucocorticoid (GC) treatment and achieved a good curative effect. Inspired by this case, we aim to systematically elaborate the pathogenesis, diagnosis, and treatment of hyperthyroidism-induced autoimmune myocarditis. Additionally, we emphasize the importance of CMRI and GC therapy in the diagnosis and treatment of hyperthyroidism-related myocarditis.
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http://dx.doi.org/10.3389/fcvm.2021.678645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292634PMC
July 2021

Multiclassification of Endoscopic Colonoscopy Images Based on Deep Transfer Learning.

Comput Math Methods Med 2021 3;2021:2485934. Epub 2021 Jul 3.

College of Information Science and Engineering, Hunan Normal University, Changsha 410081, China.

With the continuous improvement of human living standards, dietary habits are constantly changing, which brings various bowel problems. Among them, the morbidity and mortality rates of colorectal cancer have maintained a significant upward trend. In recent years, the application of deep learning in the medical field has become increasingly spread aboard and deep. In a colonoscopy, Artificial Intelligence based on deep learning is mainly used to assist in the detection of colorectal polyps and the classification of colorectal lesions. But when it comes to classification, it can lead to confusion between polyps and other diseases. In order to accurately diagnose various diseases in the intestines and improve the classification accuracy of polyps, this work proposes a multiclassification method for medical colonoscopy images based on deep learning, which mainly classifies the four conditions of polyps, inflammation, tumor, and normal. In view of the relatively small number of data sets, the network firstly trained by transfer learning on ImageNet was used as the pretraining model, and the prior knowledge learned from the source domain learning task was applied to the classification task about intestinal illnesses. Then, we fine-tune the model to make it more suitable for the task of intestinal classification by our data sets. Finally, the model is applied to the multiclassification of medical colonoscopy images. Experimental results show that the method in this work can significantly improve the recognition rate of polyps while ensuring the classification accuracy of other categories, so as to assist the doctor in the diagnosis of surgical resection.
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http://dx.doi.org/10.1155/2021/2485934DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272675PMC
July 2021

Melatonin Regulates the Periodic Growth of Cashmere by Upregulating the Expression of and β in Inner Mongolia Cashmere Goats.

Front Genet 2021 9;12:665834. Epub 2021 Jul 9.

College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.

Secondary hair follicle growth in cashmere goats has seasonal cycle changes, and melatonin (MT) has a regulatory effect on the cashmere growth cycle. In this study, the growth length of cashmere was measured by implanting MT in live cashmere goats. The results indicated that the continuous implantation of MT promoted cashmere to enter the anagen 2 months earlier and induce secondary hair follicle development. HE staining of skin tissues showed that the number of secondary hair follicles in the MT-implanted goats was significantly higher than that in the control goats ( < 0.05). Transcriptome sequencing of the skin tissue of cashmere goats was used to identify differentially expressed genes: 532 in February, 641 in October, and 305 in December. Fluorescence quantitative PCR and Western blotting results showed that MT had a significant effect on the expression of , β, and proteins in the skin tissue of Inner Mongolia cashmere goats. This finding suggested that MT alters the cycle of secondary hair follicle development by changing the expression of related genes. This research lays the foundation for further study on the mechanism by which MT regulates cashmere growth.
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http://dx.doi.org/10.3389/fgene.2021.665834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299412PMC
July 2021

Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria.

Nat Commun 2021 07 23;12(1):4347. Epub 2021 Jul 23.

Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, China.

Heterologous expression of biosynthetic gene clusters (BGCs) avails yield improvements and mining of natural products, but it is limited by lacking of more efficient Gram-negative chassis. The proteobacterium Schlegelella brevitalea DSM 7029 exhibits potential for heterologous BGC expression, but its cells undergo early autolysis, hindering further applications. Herein, we rationally construct DC and DT series genome-reduced S. brevitalea mutants by sequential deletions of endogenous BGCs and the nonessential genomic regions, respectively. The DC5 to DC7 mutants affect growth, while the DT series mutants show improved growth characteristics with alleviated cell autolysis. The yield improvements of six proteobacterial natural products and successful identification of chitinimides from Chitinimonas koreensis via heterologous expression in DT mutants demonstrate their superiority to wild-type DSM 7029 and two commonly used Gram-negative chassis Escherichia coli and Pseudomonas putida. Our study expands the panel of Gram-negative chassis and facilitates the discovery of natural products by heterologous expression.
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http://dx.doi.org/10.1038/s41467-021-24645-0DOI Listing
July 2021

Cellular senescence in knee osteoarthritis: molecular mechanisms and therapeutic implications.

Ageing Res Rev 2021 Jul 21;70:101413. Epub 2021 Jul 21.

Department of Orthopaedic Surgery, National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu, Sichuan Province, China. Electronic address:

Cellular senescence is the inability of cells to proliferate, which has both beneficial and detrimental effects on tissue development and homeostasis. Chronic accumulation of senescent cells is associated with age-related disease, including osteoarthritis, a common joint disease responsible for joint pain and disability in older adults. The pathology of this disease includes loss of cartilage, synovium inflammation, and subchondral bone remodeling. Senescent cells are present in the cartilage of people with advanced osteoarthritis, but the link between cellular senescence and this disease is unclear. In this review, we summarize current evidence for the role of cellular senescence of different cell types in the onset and progression of osteoarthritis. We focus on the underlying mechanisms of senescence in chondrocytes, which maintain the cartilage in joints, and review the role of the Forkhead family of transcription factors, which are involved in cartilage maintenance and osteoarthritis. Finally, we discuss the potential therapeutic value and implications of targeting senescent cells using senolytic agents or immune therapies, targeting the senescence-associated secretory phenotype of these cells using senomorphic agents, and renewing the plasticity of stem cells and chondrocytes. Our review highlights current gaps in understanding of the mechanism of senescence that may, when addressed, provided new options for modifying and treating disease in osteoarthritis.
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http://dx.doi.org/10.1016/j.arr.2021.101413DOI Listing
July 2021

[Pendelluft volume during double-triggered asynchronous breaths under pressure support ventilation: a prospective physiological study].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 Jun;33(6):680-685

Department of Critical Care Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China. Corresponding author: Zhou Jianxin, Email:

Objective: To investigate the relationship between double-triggering and abnormal movement of air in the lungs (pendelluft phenomenon) under pressure support ventilation (PSV).

Methods: A prospective observational study was conducted, postoperative patients admitted to department of critical care medicine of Beijing Tiantan Hospital, Capital Medical University from April 1, 2019 to August 31, 2020 and received invasive mechanical ventilation with PSV mode were enrolled. Electrical impedance tomography (EIT) monitoring was performed. Airway pressure-time, flow-time, global and regional impedance-time curves were synchronously collected and analyzed offline. The volume of abnormal movement of air in the lungs at the beginning of inspiration was measured and defined as pendelluft volume. Double-triggered breaths were identified by trained researchers. Pendelluft volume during double-triggering was measured including the first triggered breath, the double-triggered breath, and the breath immediately following the double-triggered breath. Pendelluft volume was also measured for normal breath during the study. According to the frequency of double-triggering, patients were divided into severe (≥ 1 time/min) and non-severe double-triggering group. Pendelluft volume, parameters of respiratory mechanics, and clinical outcomes between the two groups were compared.

Results: In 40 enrolled patients, a total of 9 711 breaths [(243±63) breaths/patient] were collected and analyzed, among which 222 breaths (2.3%) were identified as double-triggering. The Kappa of interobserver reliability to detect double-triggering was 0.964 [95% confidence interval (95%CI) was 0.946-0.982]. In 222 double-triggered breaths, pendelluft volume could not be measured in 7 breaths (3.2%), but the pendelluft phenomenon did exist as shown by opposite regional impedance change at the beginning of double-triggered inspiration. Finally, pendelluft volume was measured in 215 double-triggered breaths. Meanwhile, 400 normal breaths (10 normal breaths randomly selected for each patient) were identified as control. Compared with normal breath, pendelluft volume significantly increased in the first breath, the double-triggered breath, and the following normal breath [mL: 3.0 (1.4, 6.4), 8.3 (3.6, 13.2), 4.3 (1.9, 9.1) vs. 1.4 (0.7, 2.8), all P < 0.05]. Patients in severe double-triggering, pendelluft volume of normal breath and double-triggered breath were significantly higher than those in non-severe double-triggering group [mL: 1.8 (0.9, 3.2) vs. 1.1 (0.5, 2.1), P < 0.001; 8.5 (3.9, 13.4) vs. 2.0 (0.6, 9.1), P = 0.008]. Patients in severe double-triggering group had significantly higher respiratory rate than that in the non-severe double-triggering group (breaths/min: 20.9±3.5 vs. 15.2±3.7, P < 0.001). There were no significant differences in other respiratory mechanics parameters and main clinical outcomes between the two groups.

Conclusions: During PSV, the abnormal movement of air in the lungs (pendelluft phenomenon) was more likely to occur in double-triggering especially in double-triggered breath. The more frequent the double-triggering occurred, the more serious the pendelluft phenomenon was. A higher pendelluft volume of normal breath and a higher respiratory rate were related to severity of double-triggering.
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http://dx.doi.org/10.3760/cma.j.cn121430-20210115-00064DOI Listing
June 2021

Long Non-Coding RNA LINC00152 Regulates Self-Renewal of Leukemia Stem Cells and Induces Chemo-Resistance in Acute Myeloid Leukemia.

Front Oncol 2021 6;11:694021. Epub 2021 Jul 6.

Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, China.

Relapse of acute myeloid leukemia (AML) has a very poor prognosis and remains a common cause of treatment failure in patients with this disease. AML relapse is partially driven by the chemoresistant nature of leukemia stem cells (LSCs), which remains poorly understood, and our study aimed at elucidating the underlying mechanism. Accumulating evidences show that long noncoding RNAs (lncRNAs) play a crucial role in AML development. Herein, the lncRNA, LINC00152, was identified to be highly expressed in CD34 LSCs and found to regulate the self-renewal of LSCs derived from AML patients. Importantly, LINC00152 upregulation was correlated with the expression of 16 genes within a 17-gene LSC biomarker panel, which contributed to the accurate prediction of initial therapy resistance in AML. Knockdown of LINC00152 markedly increased the drug sensitivity of leukemia cells. Furthermore, LINC00152 expression was found to be correlated with poly (ADP-ribose) polymerase 1 (PARP1) expression in AML, whereas LINC00152 knockdown significantly decreased the expression of PARP1. Upregulation of LINC00152 or PARP1 was associated with poor prognosis in AML patients. Collectively, these data highlight the importance and contribution of LINC00152 in the regulation of self-renewal and chemoresistance of LSCs in AML.
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http://dx.doi.org/10.3389/fonc.2021.694021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290167PMC
July 2021

Agomelatine prevents angiotensin II-induced endothelial and mononuclear cell adhesion.

Aging (Albany NY) 2021 Jul 22;13(undefined). Epub 2021 Jul 22.

Department of Stomatology, Tibet Corps Hospital, Chinese People's Armed Police Forces, Lhasa 850000, Tibet Autonomous Region, China.

Agomelatine is a non-selective melatonin receptor agonist and an atypical antidepressant with anti-inflammatory, neuroprotective, and cardioprotective effects. The renin-angiotensin system modulates blood pressure and vascular homeostasis. Angiotensin II (Ang II) and its receptor Ang II type I receptor (ATR) are recognized as contributors to the pathogenesis of cardiovascular and cardiometabolic diseases, including diabetes, obesity, and atherosclerosis. The recruitment and attachment of monocytes to the vascular endothelium is a major event in the early stages of atherosclerosis and other cardiovascular diseases. In the present study, we demonstrate that agomelatine reduced Ang II-induced expression of ATR while significantly inhibiting the attachment of monocytes to endothelial cells induced by Ang II and mediated by ICAM-1 and VCAM-1. Additionally, Ang II inhibited the expression of the chemokines CXCL1, MCP-1, and CCL5, which are critical in the process of immune cell recruitment and invasion. Agomelatine also suppressed the expression of TNF-α, IL-8, and IL-12, which are proinflammatory cytokines that promote endothelial dysfunction and atherogenesis. Importantly, we demonstrate that the inhibitory effect of agomelatine against the expression of adhesion molecules is mediated through the downregulation of Egr-1 signaling. Together, our findings provide evidence of a novel mechanism of agomelatine that may be practicable in the treatment and prevention of cardiovascular diseases.
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http://dx.doi.org/10.18632/aging.203299DOI Listing
July 2021

Novel lncRNA lncRNA001074 participates in the low salinity-induced response in the sea cucumber Apostichopus japonicus by targeting the let-7/NKAα axis.

Cell Stress Chaperones 2021 Jul 21. Epub 2021 Jul 21.

Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Heishijiao Street, No. 52, Dalian, 116023, China.

Salinity fluctuations have severe impacts on sea cucumbers and therefore important consequences in sea cucumber farming. The responses of sea cucumbers to salinity changes are reflected in the expression profiles of multiple genes and non-coding RNAs (ncRNAs). The microRNA (let-7) which is a developmental regulator, the ion transporter gene sodium potassium ATPase gene (NKAα), and the long ncRNA lncRNA001074 were previously shown to be involved in responses to salinity changes in various marine species. To better understand the relationship between ncRNAs and target genes, the let-7/NKAα/lncRNA001074 predicted interaction was investigated in this study using luciferase reporter assays and gene knockdowns in the sea cucumber Apostichopus japonicus. The results showed that NKAα was the target gene of let-7 and NKAα expression levels were inversely correlated with let-7 expression based on the luciferase reporter assays and western blots. The let-7 abundance was negatively regulated by lncRNA001074 and NKAα both in vitro and in vivo. Knockdown of lncRNA001074 led to let-7 overexpression. These results demonstrated that lncRNA001074 binds to the 3'-UTR binding site of let-7 in a regulatory manner. Furthermore, the expression profiles of let-7, NKAα, and lncRNA001074 were analyzed in sea cucumbers after the knockdown of each of these genes. The results found that lncRNA001074 competitively bound let-7 to suppress NKAα expression under low salinity conditions. The downregulation of let-7, in conjunction with the upregulation of lncRNA001074 and NKAα, may be essential for the response to low salinity change in sea cucumbers. Therefore, the dynamic balance of the lncRNA001074, NKAα, and let-7 network might be a potential response mechanism to salinity change in sea cucumbers.
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http://dx.doi.org/10.1007/s12192-021-01207-3DOI Listing
July 2021

Uterine Artery Embolization with Small-Sized Particles for the Treatment of Symptomatic Adenomyosis: A 42-Month Clinical Follow-Up.

Int J Gen Med 2021 15;14:3575-3581. Epub 2021 Jul 15.

Medical School of Chinese PLA, Beijing, 100853, People's Republic of China.

Purpose: To assess the long-term outcome of performing uterine artery embolization (UAE) using small particles in women with symptomatic adenomyosis (AD).

Methods: Twenty-seven consecutive women (median age 42 years, range 29-53 years) with AD, in eight cases AD combined with fibroids, who underwent UAE between February 2015 and January 2019, were retrospectively analyzed. The embolization was performed using small-sized polyvinyl alcohol particles (100 μm and 300 μm). The patients completed the Uterine Fibroid Symptom and Quality of Life questionnaire at baseline and at a 42-month follow-up (range 24-71). The junction zone (JZ) thickness and uterine volume were also calculated at baseline and at a three-month follow-up.

Results: The total symptom severity score (SSS) decreased from a median of 59 (range 34-78) at baseline to a median of 9 (range 3-47) at the end of this study; the health-related quality of life score (HRQOL) increased from a median of 38 (range 23-49) at baseline to a median of 84 (range 46-97) at 42 months. Twenty of the 27 patients were asymptomatic. The clinical response of the remaining seven women was little improvement in their symptoms, and one of the seven women underwent a hysterectomy at 35 months. Twenty-six of the 27 (96%) patients had a preserved uterus at the 42-month follow-up. There was no difference after UAE in SSS, HRQOL, junction zone (JZ) thickness, and uterus volume between patients with pure AD and those with AD combined with fibroids ( = 0.729, 0.710, 0.973, and > 0.99). There was no difference in the JZ thickness and uterus volume at baseline between the asymptomatic women and the women with an insufficient response ( = 0.854 and 0.253), and there were no major complications afterwards.

Conclusion: From the long-term follow-ups, it could be seen that UAE using small particles is safe and effective in treating AD, especially in preserving the uterus. There is no relationship between the clinical outcomes and the initial presence of AD, with or without fibroids, and the JZ thickness at baseline does not seem to be a predictor for the long-term outcome of UAE.
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http://dx.doi.org/10.2147/IJGM.S312618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289687PMC
July 2021

Deubiquitinating enzyme USP30 negatively regulates mitophagy and accelerates myocardial cell senescence through antagonism of Parkin.

Cell Death Discov 2021 Jul 21;7(1):187. Epub 2021 Jul 21.

Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, 563000, Zunyi, P. R. China.

Cell senescence is associated with age-related pathological changes. Increasing evidence has revealed that mitophagy can selectively remove dysfunctional mitochondria. Overexpression of ubiquitin-specific protease 30 (USP30) has been documented to influence mitophagy and deubiquitination of mitochondrial Parkin substrates. This study was conducted to evaluate the roles of USP30 and Parkin in myocardial cell senescence and mitophagy. Initially, myocardial cells were isolated from neonatal SD rats and subjected to D-gal treatment to induce cell senescence, after which the effects of D-gal on mitochondria damage, ROS production, cell senescence, and mitophagy were assessed. The myocardial cells were infected with lentiviruses bearing overexpression plasmids or shRNA targeting Parkin or USP30 to elucidate the effects of Parkin and USP30 on D-gal-induced mitophagy damage and cell senescence. Finally, aging was induced in rats by subcutaneous injection of D-gal to determine the role of Parkin and USP30 on cell senescence in vivo. D-gal was found to trigger mitochondria damage, ROS production, and cell senescence in myocardial cells. The overexpression of Parkin or silencing of USP30 reduced D-gal-induced mitochondrial damage and relieved D-gal-induced myocardial cell senescence. Moreover, the in vivo experiments validated that either elevation of Parkin or silencing USP30 could alleviate D-gal-induced myocardial cell senescence in rats. Silencing USP30 alleviates D-gal-induced mitochondrial damage and consequently suppresses myocardial cell senescence by activating Parkin. Our study highlights the potential of USP30 as a novel target against myocardial cell senescence.
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http://dx.doi.org/10.1038/s41420-021-00546-5DOI Listing
July 2021

Phylogenetic Analysis of Strains and Identification of Serogroups by Lipopolysaccharide- and O-antigen- based PCR Assay.

Biomed Environ Sci 2021 Jun;34(6):483-488

State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102249, China;Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310021, Jiangsu, China.

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http://dx.doi.org/10.3967/bes2021.065DOI Listing
June 2021

Effects of low intensity pulsed ultrasound on expression of B-cell lymphoma-2 and BCL2-Associated X in premature ovarian failure mice induced by 4-vinylcyclohexene diepoxide.

Reprod Biol Endocrinol 2021 Jul 20;19(1):113. Epub 2021 Jul 20.

State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, 400016, Chongqing, China.

Background: Premature ovarian failure (POF) is a common disease in the field of Gynecology. Low intensity pulsed ultrasound (LIPUS) can promote tissue repair and improve function. This study was performed to determine the effects of LIPUS on granulosa cells (GCs) apoptosis and protein expression of B-cell lymphoma-2 (Bcl-2) and BCL2-Associated X (Bax) in 4-vinylcyclohexene diepoxide (VCD)-induced POF mice and investigate the mechanisms of LIPUS on ovarian function and reserve capacity.

Methods: The current POF mice model was administrated with VCD (160 mg/kg) by intraperitoneal injection for 15 consecutive days. The mice were divided into the POF group, LIPUS group and control group. In the LIPUS group, the right ovary of mice was treated by LIPUS (acoustic intensity was 200 mW/cm, frequency was 0.3 MHz, and duty cycle was 20%) for 20 min, 15 consecutive days from day 16. The mice of the POF group and control group were treated without ultrasonic output. The basic observation and body weight were recorded. Hematoxylin and eosin staining (H&E staining) and enzyme-linked immunosorbent assay (ELISA) were applied to detect ovarian follicle development, ovarian morphology and sex hormone secretion. Ovarian GCs apoptosis was detected by TUNEL assay and immunohistochemistry.

Results: The results showed that VCD can induce estrus cycle disorder, follicular atresia, sex hormone secretion decreased and GCs apoptosis in mice to establish POF model successfully. LIPUS significantly promoted follicular development, increased sex hormone secretion, inhibited excessive follicular atresia and GCs apoptosis. The mechanism might be achieved by increasing the protein expression of Bcl-2 and decreasing the expression of Bax in ovaries.

Conclusions: LIPUS can improve the POF induced by VCD. These findings have the potential to provide novel methodological foundation for the future research, which help treat POF patients in the clinic.
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http://dx.doi.org/10.1186/s12958-021-00799-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290625PMC
July 2021

Evaluation of flavor characteristics of bacon smoked with different woodchips by HS-SPME-GC-MS combined with an electronic tongue and electronic nose.

Meat Sci 2021 Jul 14;182:108626. Epub 2021 Jul 14.

College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic address:

This study investigated the effects of different woodchip types (beech, oak, pear, and apple) on the volatile compounds and sensory characteristics of smoked bacon. The volatile compounds were influenced by woodchip types and the total content of ketones and phenols in pear-smoked bacon were higher than in bacon smoked with other woodchips (P < 0.05). The E-tongue combined with E-nose can effectively distinguish the difference in the flavor of bacon smoked with different woodchip types by the signal intensities. Sensory analysis showed that smoking increased bacon's redness, saltiness, and smoky flavor compared with the control (unsmoked bacon) (P < 0.05) and it had little impact on off-odor (P > 0.05). Correlation analysis showed that the E-nose and E-tongue data were highly correlated with contents of alcohols, aldehydes, and ketones. This study revealed that the different smoked materials greatly influenced the flavor and sensory properties of bacon.
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http://dx.doi.org/10.1016/j.meatsci.2021.108626DOI Listing
July 2021

First detection of CTX-M-14-producing multidrug-resistant Salmonella enterica serotype Kentucky ST198 epidemic clone from a retail vegetable, China.

J Glob Antimicrob Resist 2021 Jul 17. Epub 2021 Jul 17.

Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou 225009, China.

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http://dx.doi.org/10.1016/j.jgar.2021.07.007DOI Listing
July 2021

Cancer cells escape p53's tumor suppression through ablation of ZDHHC1-mediated p53 palmitoylation.

Oncogene 2021 Jul 19. Epub 2021 Jul 19.

Chongqing Key Laboratory of Molecular Oncology and Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

The inactivation of tumor-suppressor genes contributes heavily to oncogenesis. The mutation of TP53 has been well-studied and recognized as a major factor in the development of tumors. Yet other means of p53 inactivation has not been well-elucidated. We previously identified a hypermethylated gene ZDHHC1 that suppresses tumor growth when the expression was restored, but the specific mechanism was yet to be found. The protein product of ZDHHC1 is an S-palmitoyltransferase and we have identified p53 as a substrate for ZDHHC1-mediated palmitoylation, specifically at the C135, C176, and C275 residues. The novel form of post-translational modification of p53 is required for the nuclear translocation of the tumor suppressor. p53 recruited DNMT3A to ZDHHC1 promoter and is responsible for the hypermethylation of ZDHHC1. The epigenetic feedback loop formed by ZDHHC1 and p53 sheds light on the inactivation of p53 without the presence of genetic mutations.
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http://dx.doi.org/10.1038/s41388-021-01949-5DOI Listing
July 2021

Stability of trivalent human papillomavirus (types 16, 18, 58) recombinant vaccine (Escherichia coli).

Chin Med J (Engl) 2021 Jul 19. Epub 2021 Jul 19.

Quality Department of Beijing Health Guard Biotechnology Inc., BDA, Daxing District, Beijing 100176, China National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China School of Chemical and Engineering, University of Chinese Academy of Sciences, Beijing 100049, China Research and Development Department of Beijing Health Guard Biotechnology Inc., BDA, Daxing District, Beijing 100176, China.

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http://dx.doi.org/10.1097/CM9.0000000000001659DOI Listing
July 2021

HTO/Cellulose Aerogel for Rapid and Highly Selective Li Recovery from Seawater.

Molecules 2021 Jul 2;26(13). Epub 2021 Jul 2.

Harbin Center for Disease Control and Prevention, Harbin 150056, China.

To achieve rapid and highly efficient recovery of Li from seawater, a series of HTiO/cellulose aerogels (HTO/CA) with a porous network were prepared by a simple and effective method. The as-prepared HTO/CA were characterized and their Li adsorption performance was evaluated. The obtained results revealed that the maximum capacity of HTO/CA to adsorb Li was 28.58 ± 0.71 mg g. The dynamic k value indicated that the Li adsorption rate of HTO/CA was nearly five times that of HTO powder. Furthermore, the aerogel retained extremely high Li selectivity compared with Mg, Ca, K, and Na. After regeneration for five cycles, the HTO/CA retained a Li adsorption capacity of 22.95 mg g. Moreover, the HTO/CA showed an excellent adsorption efficiency of 69.93% ± 0.04% and high selectivity to Li in actual seawater. These findings confirm its potential as an adsorbent for recovering Li from seawater.
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http://dx.doi.org/10.3390/molecules26134054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272140PMC
July 2021
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