Publications by authors named "Yan Su"

571 Publications

Genetic dissection of quantitative trait loci for grain size and weight by high-resolution genetic mapping in bread wheat (Triticum aestivum L.).

Theor Appl Genet 2021 Oct 13. Epub 2021 Oct 13.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.

Key Message: Six major QTLs for wheat grain size and weight were identified on chromosomes 4A, 4B, 5A and 6A across multiple environments, and were validated in different genetic backgrounds. Grain size and weight are crucial components of wheat yield. Dissection of their genetic control is thus essential for the improvement of yield potential in wheat breeding. We used a doubled haploid (DH) population to detect quantitative trait loci (QTLs) for grain width (GW), grain length (GL), and thousand grain weight (TGW) in five environments. Six major QTLs, QGw.cib-4B.2, QGl.cib-4A, QGl.cib-5A.1, QGl.cib-6A, QTgw.cib-4B, and QTgw.cib-5A, were consistently identified in at least three individual environments and in best linear unbiased prediction (BLUP) datasets, and explained 5.65-34.06% of phenotypic variation. QGw.cib-4B.2, QTgw.cib-4B, QGl.cib-5A.1 and QGl.cib-6A had no effect on grain number per spike (GNS). In addition to QGl.cib-4A, the other major QTLs were further validated by using Kompetitive Allele Specific PCR (KASP) markers in different genetic backgrounds. Moreover, significant interactions between the three major GL QTLs and two major TGW QTLs were observed. Comparison analysis showed that QGl.cib-5A.1 and QGl.cib-6A are likely new loci. Notably, QGw.cib-4B.2 and QTgw.cib-4B were co-located on chromosome 4B and improved TGW by increasing only GW, unlike nearby or overlapped loci reported previously. Three genes associated with grain development within the QGw.cib-4B.2/QTgw.cib-4B interval were identified by searches on sequence similarity, spatial expression patterns, and orthologs. The major QTLs and KASP markers reported here will be useful for elucidating the genetic architecture of grain size and weight and for developing new wheat cultivars with high and stable yield.
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http://dx.doi.org/10.1007/s00122-021-03964-2DOI Listing
October 2021

Association Between Burst-Suppression Latency and Burst-Suppression Ratio Under Isoflurane or Adjuvant Drugs With Isoflurane Anesthesia in Mice.

Front Pharmacol 2021 6;12:740012. Epub 2021 Sep 6.

Jiangsu Province Key Laboratory of Anesthesiology, Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical University, Xuzhou, China.

The same doses of anesthesia may yield varying depths of anesthesia in different patients. Clinical studies have revealed a possible causal relationship between deep anesthesia and negative short- and long-term patient outcomes. However, a reliable index and method of the clinical monitoring of deep anesthesia and detecting latency remain lacking. As burst-suppression is a characteristic phenomenon of deep anesthesia, the present study investigated the relationship between burst-suppression latency (BSL) and the subsequent burst-suppression ratio (BSR) to find an improved detection for the onset of intraoperative deep anesthesia. The mice were divided young, adult and old group treated with 1.0% or 1.5% isoflurane anesthesia alone for 2 h. In addition, the adult mice were pretreated with intraperitoneal injection of ketamine, dexmedetomidine, midazolam or propofol before they were anesthetized by 1.0% isoflurane for 2 h. Continuous frontal, parietal and occipital electroencephalogram (EEG) were acquired during anesthesia. The time from the onset of anesthesia to the first occurrence of burst-suppression was defined as BSL, while BSR was calculated as percentage of burst-suppression time that was spent in suppression periods. Under 1.0% isoflurane anesthesia, we found a negative correlation between BSL and BSR for EEG recordings obtained from the parietal lobes of young mice, from the parietal and occipital lobes of adult mice, and the occipital lobes of old mice. Under 1.5% isoflurane anesthesia, only the BSL calculated from EEG data obtained from the occipital lobe was negatively correlated with BSR in all mice. Furthermore, in adult mice receiving 1.0% isoflurane anesthesia, the co-administration of ketamine and midazolam, but not dexmedetomidine and propofol, significantly decreased BSL and increased BSR. Together, these data suggest that BSL can detect burst-suppression and predict the subsequent BSR under isoflurane anesthesia used alone or in combination with anesthetics or adjuvant drugs. Furthermore, the consistent negative correlation between BSL and BSR calculated from occipital EEG recordings recommends it as the optimal position for monitoring burst-suppression.
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http://dx.doi.org/10.3389/fphar.2021.740012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504134PMC
September 2021

l-Cysteine-Modified Graphene Oxide-Based Membrane for Chiral Selective Separation.

ACS Appl Mater Interfaces 2021 Oct 9. Epub 2021 Oct 9.

School of Chemistry and Chemical Engineering, Shandong University, 27 Shanda Nanlu, Jinan 250100, PR China.

A novel chiral separation membrane was fabricated by assembling l-cysteine (l-Cys)-modified graphene oxide sheets. l-Cys modification leads to an enantiomer separation membrane with an accessible interlayer spacing of 8 Å, which allows high solvent permeability. In the racemate separation experiments under isobaric conditions, the enantiomeric excess (ee) values of alanine (Ala), threonine (Thr), tyrosine (Tyr), and penicillamine (Pen) racemates in the permeation solution were 43.60, 44.11, 27.43, and 46.44%, respectively. In the racemate separation experiments under negative pressure, the separation performances of Ala, Thr, and Tyr were still maintained, and the enantiomeric excess (ee) values of the filtrate after separation were 56.80, 54.57, and 32.34%, respectively. These results indicate that the as-prepared GO-Cys membrane has a great practical value in the field of enantiomer separation.
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http://dx.doi.org/10.1021/acsami.1c14900DOI Listing
October 2021

Enhanced Chlorinated Pollutant Degradation by the Synergistic Effect between Dechlorination and Hydroxyl Radical Oxidation on a Bimetallic Single-Atom Catalyst.

Environ Sci Technol 2021 Oct 7. Epub 2021 Oct 7.

Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.

Chlorinated organic pollutants are highly toxic and widespread in the environment, which cause ecological risk and threaten the human health. Chlorinated pollutants are difficult to degrade and mineralize by the conventional advanced oxidation process as the C-Cl bond is resistant to reactive oxygen species oxidation. Herein, we designed a bifunctional Fe/Cu bimetallic single-atom catalyst anchored on N-doped porous carbon (FeCuSA-NPC) for the electro-Fenton process, in which chlorinated pollutants are dechlorinated on single-atom Cu and subsequently oxidized by the ·OH radical produced from O conversion on single-atom Fe. Benefitting from the synergistic effect between dechlorination on single-atom Cu and ·OH oxidation on single-atom Fe, the chlorinated organic pollutants can be efficiently degraded and mineralized. The mass activity for chlorinated organic pollutant degradation by FeCuSA-NPC is 545.1-1374 min g, excessing the highest value of the reported electrocatalyst. Moreover, FeCuSA-NPC is demonstrated to be pH-universal, long-term stable, and environment friendly. This work provides a new insight into the rational design of a bifunctional electrocatalyst for efficient removal of chlorinated organic pollutants.
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http://dx.doi.org/10.1021/acs.est.1c04943DOI Listing
October 2021

Genome-Wide Identification and Analyses of Drought/Salt-Responsive Cytochrome Genes in .

Int J Mol Sci 2021 Sep 15;22(18). Epub 2021 Sep 15.

Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Cytochrome P450 monooxygenases (P450s) catalyze a great number of biochemical reactions and play vital roles in plant growth, development and secondary metabolism. As yet, the genome-scale investigation on is still lacking in the model legume . In particular, whether and how many are involved in drought and salt stresses for growth, development and yield remain unclear. In this study, a total of 346 genes were identified and classified into 10 clans containing 48 families. Among them, sixty-one genes pairs are tandem duplication events and 10 genes are segmental duplication events. genes within one family exhibit high conservation and specificity in intron-exon structure. Meanwhile, many genes displayed tissue-specific expression pattern in various tissues. Specifically, the expression pattern of 204 genes under drought/NaCl treatments were analyzed by using the weighted correlation network analysis (WGCNA). Among them, eight genes (, , , , , , , and ), and six genes (, , , , , and ) were found to be hub genes under drought/NaCl treatments, respectively. The expression levels of these selected hub genes could be induced, respectively, by drought/NaCl treatments, as validated by qPCR analyses, and most of these genes are involved in the secondary metabolism and fatty acid pathways. The genome-wide identification and co-expression analyses of superfamily genes established a gene atlas for a deep and systematic investigation of genes in , and the selected drought-/salt-responsive genes could be utilized for further functional characterization and molecular breeding for resistance in legume crops.
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http://dx.doi.org/10.3390/ijms22189957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467197PMC
September 2021

Neuroblastoma and DIPG Organoid Coculture System for Personalized Assessment of Novel Anticancer Immunotherapies.

J Pers Med 2021 Aug 30;11(9). Epub 2021 Aug 30.

Princess Máxima Center for Paediatric Oncology, Heidelberglaan 25, 3584 CS Utrecht, The Netherlands.

Cancer immunotherapy has transformed the landscape of adult cancer treatment and holds a great promise to treat paediatric malignancies. However, in vitro test coculture systems to evaluate the efficacy of immunotherapies on representative paediatric tumour models are lacking. Here, we describe a detailed procedure for the establishment of an ex vivo test coculture system of paediatric tumour organoids and immune cells that enables assessment of different immunotherapy approaches in paediatric tumour organoids. We provide a step-by-step protocol for an efficient generation of patient-derived diffuse intrinsic pontine glioma (DIPG) and neuroblastoma organoids stably expressing eGFP-ffLuc transgenes using defined serum-free medium. In contrast to the chromium-release assay, the new platform allows for visualization, monitoring and robust quantification of tumour organoid cell cytotoxicity using a non-radioactive assay in real-time. To evaluate the utility of this system for drug testing in the paediatric immuno-oncology field, we tested our in vitro assay using a clinically used immunotherapy strategy for children with high-risk neuroblastoma, dinutuximab (anti-GD2 monoclonal antibody), on GD2 proficient and deficient patient-derived neuroblastoma organoids. We demonstrated the feasibility and sensitivity of our ex vivo coculture system using human immune cells and paediatric tumour organoids as ex vivo tumour models. Our study provides a novel platform for personalized testing of potential anticancer immunotherapies for aggressive paediatric cancers such as neuroblastoma and DIPG.
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http://dx.doi.org/10.3390/jpm11090869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466534PMC
August 2021

7S,15R-Dihydroxy-16S,17S-Epoxy-Docosapentaenoic Acid, a Novel DHA Epoxy Derivative, Inhibits Colorectal Cancer Stemness through Repolarization of Tumor-Associated Macrophage Functions and the ROS/STAT3 Signaling Pathway.

Antioxidants (Basel) 2021 Sep 14;10(9). Epub 2021 Sep 14.

Microbial Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si 56212, Korea.

Colorectal cancer is a highly malignant cancer that is inherently resistant to many chemotherapeutic drugs owing to the complicated tumor-supportive microenvironment (TME). Tumor-associated macrophages (TAM) are known to mediate colorectal cancer metastasis and relapse and are therefore a promising therapeutic target. In the current study, we first confirmed the anti-inflammatory effect of 7S,15R-dihydroxy-16S,17S-epoxy-docosapentaenoic acid (diHEP-DPA), a novel DHA dihydroxy derivative synthesized in our previous work. We found that diHEP-DPA significantly reduced lipopolysaccharide (LPS)-induced inflammatory cytokines secretion of THP1 macrophages, IL-6, and TNF-α. As expected, diHEP-DPA also modulated TAM polarization, as evidenced by decreased gene and protein expression of the TAM markers, CD206, CD163, VEGF, and TGF-β1. During the polarization process, diHEP-DPA treatment decreased the concentration of TGF-β1, IL-1β, IL-6, and TNF-α in culture supernatants via inhibiting the NF-κB pathway. Moreover, diHEP-DPA blocked immunosuppression by reducing the expression of SIRPα in TAMs and CD47 in colorectal cancer cells. Knowing that an inflammatory TME largely serves to support epithelial-mesenchymal transition (EMT) and cancer stemness, we tested whether diHEP-DPA acted through polarization of TAMs to regulate these processes. The intraperitoneally injected diHEP-DPA inhibited tumor growth when administered alone or in combination with 5-fluorouracil (5-FU) chemotherapy in vivo. We further found that diHEP-DPA effectively reversed TAM-conditioned medium (TCCM)-induced EMT and enhanced colorectal cancer stemness, as evidenced by its inhibition of colorectal cancer cell migration, invasion and expression of EMT markers, as well as cancer cell tumorspheres formation, without damaging colorectal cancer cells. DiHEP-DPA reduced the population of aldehyde dehydrogenase (ALDH)-positive cells and expression of colorectal stemness marker proteins (CD133, CD44, and Sox2) by modulating TAM polarization. Additionally, diHEP-DPA directly inhibited cancer stemness by inducing the production of reactive oxygen species (ROS), which, in turn, reduced the phosphorylation of nuclear signal transducer and activator of transcription 3 (STAT3). These data collectively suggest that diHEP-DPA has the potential for development as an anticancer agent against colorectal cancer.
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http://dx.doi.org/10.3390/antiox10091459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470250PMC
September 2021

Abnormalities of Cortical Morphology and Structural Covariance Network in Patients with Subacute Basal Ganglia Stroke.

Acad Radiol 2021 Sep 20. Epub 2021 Sep 20.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China. Electronic address:

Rationale And Objectives: The direct damage caused by ischemic stroke is relatively localized, but structural reorganization of cortical regions could occur across the brain. Changes of large-scale, cortical structural brain networks after basal ganglia stroke are less well reported. We, therefore, aim to explore the abnormalities of cortical morphology and structural network topology in patients with unilateral basal ganglia stroke during the subacute period.

Materials And Methods: Thirty patients with first-ever basal ganglia stroke and thirty age- and sex-matched healthy controls were recruited for our analysis. Patients underwent structural magnetic resonance imaging examinations and clinical assessment from seven days to three months post-stroke. Alterations in cortical morphology and topological properties of the cortical structural network were measured respectively using the surface-based morphology and graph-theoretical methods.

Results: We observed focal cortical atrophy, specifically in areas of frontal and temporal cortices. Moreover, the cortical thickness in the contralesional transverse temporal gyrus and superior temporal gyrus was positively correlated with cognitive function scores. Network analysis revealed that patients with basal ganglia stroke showed increased clustering coefficient, increased mean local efficiency as well as a reorganization of degree-based hubs. In addition, these patients also showed reduced robustness under a random attack compared to healthy controls.

Conclusion: These findings indicated a unique pattern of cortical reorganization and the abnormal topological organization of cortical thickness-based structural covariance networks in patients with basal ganglia stroke, which is beneficial to understand the pathophysiological mechanisms of functional disorders at the cortical structural network level and find potential targets for induced neuromodulation.
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http://dx.doi.org/10.1016/j.acra.2021.08.011DOI Listing
September 2021

Plasmonic Oxygen Defects in MO (M = W or Mo) Nanomaterials: Synthesis, Modifications, and Biomedical Applications.

Adv Healthc Mater 2021 Sep 22:e2101331. Epub 2021 Sep 22.

Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai, 200444, China.

Nanomedicine is a promising technology with many advantages and provides exciting opportunities for cancer diagnosis and therapy. During recent years, the newly developed oxygen-deficiency transition metal oxides MO (M = W or Mo) have received significant attention due to the unique optical properties, such as strong localized surface plasmon resonance (LSPR) , tunable and broad near-IR absorption, high photothermal conversion efficiency, and large X-ray attenuation coefficient. This review presents an overview of recent advances in the development of MO nanomaterials for biomedical applications. First, the fundamentals of the LSPR effect are introduced. Then, the preparation and modification methods of MO nanomaterials are summarized. In addition, the biological effects of MO nanomaterials are highlighted and their applications in the biomedical field are outlined. This includes imaging modalities, cancer treatment, and antibacterial capability. Finally, the prospects and challenges of MO and MO -based nanomaterial for fundamental studies and clinical applications are also discussed.
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http://dx.doi.org/10.1002/adhm.202101331DOI Listing
September 2021

Efficient production of xylooligosaccharides rich in xylobiose and xylotriose from poplar by hydrothermal pretreatment coupled with post-enzymatic hydrolysis.

Bioresour Technol 2021 Sep 15;342:125955. Epub 2021 Sep 15.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, People's Republic of China. Electronic address:

A promising approach for production of value-added xylooligosaccharides (XOS) from poplar was developed by combining hydrothermal pretreatment and endo-xylanase post-hydrolysis. Results showed that the 35.4% XOS (DP 2-6) and 17.6% low DP xylans (DP > 6) were obtained at the identified optimal condition (170 °C, 50 min) for hydrothermal pretreatment. Structural features of low DP xylans generated during the hydrothermal pretreatment were examined, revealing that low DP xylans are mainly comprised of 4-O-methylglucuronic xylan and are involved in lignin carbohydrate complexes. Moreover, higher pretreatment intensity promoted the cleavage of side-chain substituents including arabinose and glucuronic acid groups. The subsequent endo-xylanase hydrolysis of the pretreatment liquor hydrolyzed low DP xylans, contributing to a significant improvement in xylobiose and xylotriose proportions. This combined strategy resulted in a XOS with conversion yield of 44.6% containing 78.7% xylobiose and xylotriose starting from the initial xylan in raw poplar.
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http://dx.doi.org/10.1016/j.biortech.2021.125955DOI Listing
September 2021

Design of Persistent and Stable Porous Radical Polymers by Electronic Isolation Strategy.

Angew Chem Int Ed Engl 2021 Sep 15. Epub 2021 Sep 15.

State Key Laboratory of Luminescent Materials and Devices, Institute of Polymer Optoelectronic Materials and Devices, South China University of Technology, Guangzhou, 510640, P. R. China.

Conjugated organic radical polymers with stable radical features are difficult to design because the π conjugation in the polymer backbones makes the radicals readily delocalize and tend to undergo covalent bonding processes. In this work, we report an electronic isolation strategy to design stable porous radical polymers by homocoupling reaction from a meta-position active monomer. The meta linkage ensures less conjugation in the polymer skeletons, localizes the resonant radicals, and prevents them from recombination. The resulting porous radical polymer exhibits exceptional radical characters with ultralow band gap of 0.68 eV, strong yet extended UV/Vis-NIR absorption up to 1800 nm, and high spin density. The above features make the polymer very promising in the photothermal conversion with record-high photothermal temperature increment of ≈∼240 °C and striking solar-driven water evaporation efficiency of 96.8 %. Our results demonstrate the feasibility of electronic isolation of radicals for producing outstanding photothermal materials.
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http://dx.doi.org/10.1002/anie.202108318DOI Listing
September 2021

Tractometry-Based Estimation of Corticospinal Tract Injury to Assess Initial Impairment and Predict Functional Outcomes in Ischemic Stroke Patients.

J Magn Reson Imaging 2021 Sep 6. Epub 2021 Sep 6.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Corticospinal tract (CST) injury has been shown to exert a major influence on functional recovery after ischemic stroke.

Purpose: To evaluate the prognostic value of CST injury estimated using a recent developed tractometry-based method.

Study Type: Prospective.

Population: Forty-eight patients with CST damage induced by stroke lesion who underwent brain magnetic resonance imaging within 7 days from onset.

Sequence: Diffusion-weighted imaging (b = 1000 seconds/mm ) and diffusion kurtosis imaging (DKI) spin-echo echo-planar sequence with three b-values (0, 1250, and 2500 seconds/mm ) at 3.0 T.

Assessment: A recently developed approach that combines tract segmentation and orientation mapping was used for CST-specific tractography and tractometry. CST injury was estimated using the proposed method with diffusion metrics extracted from DKI sequence and with the first principal component (PC1) of the metrics. We also calculated the weighted lesion load (wLL) for comparison. Clinical evaluation included the National Institutes of Health Stroke Score in the acute phase and the modified Rankin scale at 3 months post-stroke. The correlations between CST injury and initial motor impairment, as well as the prognostic values of CST injury for functional outcomes were evaluated.

Statistical Tests: Pearson correlation and logistic regression. Area under the receiver operating characteristic curve. P < 0.05 was considered statistically significant.

Results: CST injury calculated with diffusion metrics except fractional anisotropy all showed significant correlations with initial motor impairment. PC1 achieved the largest correlation coefficient (R = 0.65) compared with wLL and other diffusion metrics. In addition to wLL, DKI_AK, AFD_total, and PC1 maximum all showed predictive values for functional outcomes.

Data Conclusion: Structural injury to CST is important for the assessment of the extent of injury and the prediction of functional outcome. The method proposed in our study could provide an imaging indicator to quantify the CST injury after ischemic stroke.

Level Of Evidence: 2 TECHNICAL EFFICACY: Stage 1.
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http://dx.doi.org/10.1002/jmri.27911DOI Listing
September 2021

The Spatiotemporal Evolution of MRI-Derived Oxygen Extraction Fraction and Perfusion in Ischemic Stroke.

Front Neurosci 2021 16;15:716031. Epub 2021 Aug 16.

Department of Radiology, Weill Cornell Medicine, New York, NY, United States.

Purpose: This study aimed to assess the spatiotemporal evolution of oxygen extraction fraction (OEF) in ischemic stroke with a newly developed cluster analysis of time evolution (CAT) for a combined quantitative susceptibility mapping and quantitative blood oxygen level-dependent model (QSM + qBOLD, QQ).

Method: One hundred and fifteen patients in different ischemic stroke phases were retrospectively collected for measurement of OEF of the infarcted area defined on diffusion-weighted imaging (DWI). Clinical severity was assessed using the National Institutes of Health Stroke Scale (NIHSS). Of the 115 patients, 11 underwent two longitudinal MRI scans, namely, three-dimensional (3D) multi-echo gradient recalled echo (mGRE) and 3D pseudo-continuous arterial spin labeling (pCASL), to evaluate the reversal region (RR) of the initial diffusion lesion (IDL) that did not overlap with the final infarct (FI). The temporal evolution of OEF and the cerebral blood flow (CBF) in the IDL, the RR, and the FI were assessed.

Results: Compared to the contralateral mirror area, the OEF of the infarcted region was decreased regardless of stroke phases ( < 0.05) and showed a declining tendency from the acute to the chronic phase ( = 0.022). Five of the 11 patients with longitudinal scans showed reversal of the IDL. Relative oxygen extraction fraction (rOEF, compared to the contralateral mirror area) of the RR increased from the first to the second MRI ( = 0.044). CBF was about 1.5-fold higher in the IDL than in the contralateral mirror area in the first MRI. Two patients showed penumbra according to the enlarged FI volume. The rOEF of the penumbra fluctuated around 1.0 at earlier scan times and then decreased, while the CBF decreased continuously.

Conclusion: The spatiotemporal evolution of OEF and perfusion in ischemic lesions is heterogeneous, and the CAT-based QQ method is feasible to capture cerebral oxygen metabolic information.
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http://dx.doi.org/10.3389/fnins.2021.716031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415351PMC
August 2021

Behavior change factors and retention in dietary interventions for older adults: scoping review.

Gerontologist 2021 Sep 3. Epub 2021 Sep 3.

Department of Psychology, University of Nevada, Las Vegas, Nevada, USA.

Background And Objectives: Although poor diet is a major driver of morbidity and mortality in people 60 and older, few dietary interventions are widely implemented for this population. We mapped behavior change theories, agents, and techniques in dietary interventions for adults 60+ and explored relationships between these factors and ability to retain at least 80% of the study participants.

Research Design And Methods: We conducted a scoping review using MEDLINE, CINAHL, and Web of Science through April 2021 for dietary interventions in adults 60 and older. We collated, summarized, and calculated frequency distributions of behavior change theories, behavior change agents, and behavior change techniques (BCTs) using BCTv1 taxonomy with regard to participant retention across 43 studies.

Results: Only 49% and 30% of the studies reported behavior theory and change agents respectively. Of the studies reporting on theory and agents, the most common were social cognitive theory and the related mechanism of self-efficacy. The most common BCTv1 were "shaping knowledge" and "goals and planning." Several BCTv1 such as "antecedents" and "reward and threat" and evidence for concordance between BCTs and change agents were more common in interventions with higher retention rates.

Discussion And Implications: Mechanistically concordant studies with BCTs that involve resource allocation and positive reinforcement through rewards may be advantageous for retention in dietary intervention for older adults. Future studies should continue developing theory and mechanism-oriented research. Furthermore, future studies should consider diversifying the portfolio of currently deployed BCTs and strengthening a concordance between BCTs and mechanisms of change.
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http://dx.doi.org/10.1093/geront/gnab133DOI Listing
September 2021

Differences in Wall Shear Stress Between High-Risk and Low-Risk Plaques in Patients With Moderate Carotid Artery Stenosis: A 4D Flow MRI Study.

Front Neurosci 2021 11;15:678358. Epub 2021 Aug 11.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

This study aimed to evaluate the difference in wall shear stress (WSS) (axial, circumferential, and 3D) between high-risk and low-risk plaques in patients with moderate carotid artery stenosis and to identify which time points and directions play the dominant roles in determining the risk associated with plaques. Forty carotid arteries in 30 patients were examined in this study. All patients underwent high-resolution vessel wall (HRVW) imaging, diffusion-weighted imaging (DWI), and 4D flow MRI; HRVW imaging and DWI were used to separate low- and high-risk plaque. Twenty-four high-risk plaques and 16 low-risk plaques were enrolled. An independent-sample -test was used to compare WSS between low- and high-risk plaques in the whole cardiac cycle and at 20 different time points in the cardiac cycle. The study found that patients with high-risk plaques had higher WSS than those with low-risk plaques throughout the entire cardiac cycle ( < 0.05), but the changes varied at the 20 different time points. The number of non-significant differences ( > 0.05) was less in diastole than in systole across different time points. The axial WSS values were higher than the circumferential WSS values; the difference in axial WSS values between high- and low-risk plaques was more significant than the difference in circumferential WSS, whereas 3D WSS values best reflected the difference between high-risk and low-risk plaques because they showed significant differences at every time point. In conclusion, increased WSS, especially during the diastolic period and in the axial direction, may be a signal of a high-risk plaque and may cause cerebrovascular events in patients with moderate carotid artery stenosis. Additionally, WSS can provide hemodynamic information and help clinicians make more appropriate decisions for patients with plaques.
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http://dx.doi.org/10.3389/fnins.2021.678358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385133PMC
August 2021

Comprehensive mapping of SARS-CoV-2 interactions in vivo reveals functional virus-host interactions.

Nat Commun 2021 08 25;12(1):5113. Epub 2021 Aug 25.

Epigenetic and Epitranscriptomic Regulation, Genome Institute of Singapore, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.

SARS-CoV-2 is a major threat to global health. Here, we investigate the RNA structure and RNA-RNA interactions of wildtype (WT) and a mutant (Δ382) SARS-CoV-2 in cells using Illumina and Nanopore platforms. We identify twelve potentially functional structural elements within the SARS-CoV-2 genome, observe that subgenomic RNAs can form different structures, and that WT and Δ382 virus genomes fold differently. Proximity ligation sequencing identify hundreds of RNA-RNA interactions within the virus genome and between the virus and host RNAs. SARS-CoV-2 genome binds strongly to mitochondrial and small nucleolar RNAs and is extensively 2'-O-methylated. 2'-O-methylation sites are enriched in viral untranslated regions, associated with increased virus pair-wise interactions, and are decreased in host mRNAs upon virus infection, suggesting that the virus sequesters methylation machinery from host RNAs towards its genome. These studies deepen our understanding of the molecular and cellular basis of SARS-CoV-2 pathogenicity and provide a platform for targeted therapy.
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http://dx.doi.org/10.1038/s41467-021-25357-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387478PMC
August 2021

Berberine Attenuates MPP-Induced Neuronal Injury by Regulating LINC00943/miR-142-5p/KPNA4/NF-κB Pathway in SK-N-SH Cells.

Neurochem Res 2021 Aug 24. Epub 2021 Aug 24.

Department of Geriatrics, Yantai Affiliated Hospital of Binzhou Medical University, No.717, Jinbu Street, MuPing District, Yantai, 264100, Shandong, China.

Berberine plays a neuro-protective role in neurodegenerative diseases, including Parkinson's disease (PD). Long non-coding RNAs (lncRNAs) play critical roles in PD pathogenesis. The purpose of this study was to investigate whether LINC00943 was involved in the role of berberine in PD. 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) or 1-methyl-4-phenyl pyridine (MPP) were used to construct PD mouse and cell models, respectively. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8) and 5-Ethynyl-2'-deoxyuridine (Edu) assays. Inflammation and cell apoptosis were assessed by enzyme-linked immunosorbent assay (ELISA) and flow cytometry, respectively. Quantitative real-time PCR (qRT-PCR) was employed to test the expression of LINC00943, microRNA (miR)-142-5p, and karyopherin subunit alpha 4 (KPNA4) mRNA. The protein levels of NF-κB pathway-related markers and KPNA4 were measured by western blot. Oxidative stress level was assessed by corresponding kits. The interaction between miR-142-5p and LINC00943 or KPNA4 was determined via dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Berberine inhibited MPP-induced injury in SK-N-SH cells by promoting cell proliferation and suppressing inflammation, apoptosis, and oxidative injury. LINC00943 and KPNA4 were upregulated and miR-142-5p was downregulated in PD mouse and cell models. LINC00943 (or KPNA4) overexpression or miR-142-5p inhibition abated the neuro-protective role of berberine in PD cell model. Moreover, miR-142-5p was a target of LINC00943, and KPNA4 could specially bind to miR-142-5p. Additionally, berberine inhibited NF-κB pathway by regulating LINC00943/miR-142-5p/KPNA4 axis. Berberine protected SK-N-SH cell from MPP-induced neuronal damage via regulating LINC00943/miR-142-5p/KPNA4/NF-κB pathway, highlighting novel evidence for the neuro-protective role of berberine in PD.
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http://dx.doi.org/10.1007/s11064-021-03431-wDOI Listing
August 2021

Synergistic effects of hydrothermal and deep eutectic solvent pretreatment on co-production of xylo-oligosaccharides and enzymatic hydrolysis of poplar.

Bioresour Technol 2021 Dec 17;341:125787. Epub 2021 Aug 17.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, People's Republic of China.

Full utilization of lignocellulose is critical for its biorefinery development. In this study, a sustainable biorefinery process based upon poplar sawdust was established using sequential hydrothermal and deep eutectic solvent treatment (HP-DES). Results showed that single hydrothermal pretreatment (HP) could produce 53.2% xylo-oligosaccharides (XOS) (based on raw xylan), while the enzymatic digestibility was low. Conversely, single DES treatment achieved effective enzymatic digestibility but low XOS yields. As compared to HP, both DES treatment and HP-DES showed high selectivity for lignin removal and high glucose yield. Surprisingly, most of HP-DES residues had obviously lower enzymatic digestibilities than those of single DES residues. This was mainly explained by the differences of the surface lignin contents between DES and HP-DES residues. Moreover, nearly complete enzymatic hydrolysis of HP-DES residues was achieved with the addition of bovine serum albumin. This work demonstrated this HP-DES yielded XOS, fermentable sugar, and pure lignin with high processibility.
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http://dx.doi.org/10.1016/j.biortech.2021.125787DOI Listing
December 2021

Large-Area, Flexible, Transparent, and Long-Lived Polymer-Based Phosphorescence Films.

J Am Chem Soc 2021 Sep 19;143(34):13675-13685. Epub 2021 Aug 19.

Divisions of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.

Polymer-based room-temperature phosphorescence (RTP) materials with high flexibility and large-area producibility are highly promising for applications in organic electronics. However, achieving such photophysical materials is challenging because of difficulties in populating and stabilizing susceptible triplet excited states at room temperature. Herein large-area, flexible, transparent, and long-lived RTP systems prepared by doping rationally selected organic chromophores in a poly(vinyl alcohol) (PVA) matrix were realized through a hydrogen-bonding and coassembly strategy. In particular, the 3,6-diphenyl-9-carbazole (DPCz)-doped PVA film shows long-lived phosphorescence emission (up to 2044.86 ms) and a remarkable duration of afterglow (over 20 s) under ambient conditions. Meanwhile, the 7-dibenzo[,]carbazole (DBCz)-doped PVA film exhibits high absolute luminance of 158.4 mcd m after the ultraviolet excitation source is removed. The RTP results not only from suppressing the nonradiative decay by abundant hydrogen-bonding interactions in the PVA matrix but also from minimizing the energy gap (Δ) between the singlet state and the triplet state through the coassembly effect. On account of the outstanding mechanical properties and the afterglow performance of these RTP materials, they were applied in the fabrication of flexible 3D objects with repeatable folding and curling properties. Importantly, the multichannel afterglow light-emitting diode arrays were established under ambient conditions. The present long-lived phosphorescent systems demonstrate a bright opportunity for the production of large-area, flexible, and transparent emitting materials.
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http://dx.doi.org/10.1021/jacs.1c05213DOI Listing
September 2021

Effects of Smoking on Inflammatory Markers in a Healthy Population as Analyzed the Gut Microbiota.

Front Cell Infect Microbiol 2021 23;11:633242. Epub 2021 Jul 23.

Health Management Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

The number of people who smoke has increased in recent years, and the incidence of smoking-related diseases increases annually. This study was conducted to explore whether smoking affects diseases changes in the gut microbiota. We enrolled 33 smokers and 121 non-smokers. We collected fecal samples from all participants and performed whole-genome sequencing. Smoking significantly affected the gut microbiota. At the phylum through genus levels, the smokers' microbiotas showed slight changes compared with those of the non-smokers. The α- and β-diversities differed significantly between the smokers and non-smokers, and the smokers' gut microbiota compositions differed significantly from those of the non-smokers. At the species level, the relative abundances of (=0.00197) and (=0.0468) were significantly greater in the smokers than in the non-smokers, while the relative abundances of (=0.0000052) and (=0.0057) were significantly lower in the smokers. Smoking increases inflammation in the body by inducing an increased abundance of proinflammatory bacteria. Non-smokers had higher abundances of anti-inflammatory microorganisms than did smokers; these microorganisms can produce short-chain fatty acids, which inhibit inflammation.
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http://dx.doi.org/10.3389/fcimb.2021.633242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342938PMC
August 2021

A Metagenome-Wide Association Study of the Gut Microbiome and Metabolic Syndrome.

Front Microbiol 2021 16;12:682721. Epub 2021 Jul 16.

Health Management Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Metabolic syndrome (MetS) is a wide-ranging disorder, which includes insulin resistance, altered glucose and lipid metabolism, and increased blood pressure and visceral obesity. MetS symptoms combine to result in a significant increase in cardiovascular risk. It is therefore critical to treat MetS in the early stages of the disorder. In this study, 123 MetS patients and 304 controls were recruited to determine whether the gut microbiome plays a role in MetS development and progression. By using whole-genome shotgun sequencing, we found that the gut microbiomes of MetS patients were different from those of controls, with MetS patients possessing significantly lower gut microbiome diversity. In addition, 28 bacterial species were negatively correlated with waist circumstance, with showing the strongest correlation, followed by , , , , and . These species were also enriched in controls relative to MetS patients. In addition, pathways involved in the biosynthesis of carbohydrates, fatty acids, and lipids were enriched in the MetS group, indicating that microbial functions related to fermentation may play a role in MetS. We also found that microbiome changes in MetS patients may aggravate inflammation and contribute to MetS diseases by inhibiting the production of short-chain fatty acids (SCFAs). Taken together, these results indicate the potential utility of beneficial gut microbiota as a potential therapeutic to alleviate MetS.
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http://dx.doi.org/10.3389/fmicb.2021.682721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322780PMC
July 2021

Replication Stress Response Modifies Sarcomeric Cardiomyopathy Remodeling.

J Am Heart Assoc 2021 08 29;10(15):e021768. Epub 2021 Jul 29.

Division of Cardiology Department of Medicine Heart, Lung Blood and Vascular Medicine InstituteSchool of MedicineUniversity of PittsburghUniversity of Pittsburgh Medical Center PA.

Background Sarcomere gene mutations lead to cardiomyocyte hypertrophy and pathological myocardial remodeling. However, there is considerable phenotypic heterogeneity at both the cellular and the organ level, suggesting modifiers regulate the effects of these mutations. We hypothesized that sarcomere dysfunction leads to cardiomyocyte genotoxic stress, and this modifies pathological ventricular remodeling. Methods and Results Using a murine model deficient in the sarcomere protein, Mybpc3 (cardiac myosin-binding protein 3), we discovered that there was a surge in cardiomyocyte nuclear DNA damage during the earliest stages of cardiomyopathy. This was accompanied by a selective increase in ataxia telangiectasia and rad3-related phosphorylation and increased p53 protein accumulation. The cause of the DNA damage and DNA damage pathway activation was dysregulated cardiomyocyte DNA synthesis, leading to replication stress. We discovered that selective inhibition of ataxia telangiectasia and rad3 related or cardiomyocyte deletion of p53 reduced pathological left ventricular remodeling and cardiomyocyte hypertrophy in Mybpc3 animals. Mice and humans harboring other types of sarcomere gene mutations also had evidence of activation of the replication stress response, and this was associated with cardiomyocyte aneuploidy in all models studied. Conclusions Collectively, our results show that sarcomere mutations lead to activation of the cardiomyocyte replication stress response, which modifies pathological myocardial remodeling in sarcomeric cardiomyopathy.
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http://dx.doi.org/10.1161/JAHA.121.021768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475701PMC
August 2021

Emerging Threat of Multidrug Resistant Pathogens From Neonatal Sepsis.

Front Cell Infect Microbiol 2021 12;11:694093. Epub 2021 Jul 12.

Department of Laboratory Medicine, Chongqing Health Center for Women and Children, Chongqing, China.

Multidrug-resistant (MDR) pathogens are responsible for a substantial burden of morbidity and mortality from neonatal sepsis; however, data on these sepsis-related pathogens among hospitalized neonates in China are not well characterized. In this study, a total of 240 strains were isolated from four Women and Children's hospitals in Southwest China between 2014 and 2019. Of these included pathogens, 104 (43.33%) were gram-positive bacteria, 129 (53.75%) were gram-negative bacteria, and 7 (2.92%) were fungi. (, 34.01%) and (, 15.35%) were the main pathogen of neonate bacteremia. ST167 were the most prevalent STs in and ST11 in Our study found that (62.71%) was the predominate pathogen of early-onset sepsis, among which 64.86% were MDR. Late-onset sepsis was mainly caused by (28.31%) and (24.78%), with showing that 78.33% of these pathogens were MDR. Notably, the prevalence of EO/LO pathogens were quite different from Indian and south of China. Moreover, we found that (42.06%) was most dominant resistant genes with about a third isolates (31.09%) were positive for . All the carbapenem-resistant were positive for NDM-1. Moreover, late-onset sepsis and antibiotic exposure were significantly associated with MDR infection. Emerging multi-resistant pathogens of sepsis posts a serious threat to neonatal outcomes and emphasizes an urgent need to control their further spread.
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http://dx.doi.org/10.3389/fcimb.2021.694093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312093PMC
August 2021

Identification and validation of two major QTLs for spike compactness and length in bread wheat (Triticum aestivum L.) showing pleiotropic effects on yield-related traits.

Theor Appl Genet 2021 Nov 26;134(11):3625-3641. Epub 2021 Jul 26.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.

Key Message: Two major and stable QTLs for spike compactness and length were detected and validated in multiple genetic backgrounds and environments, and their pleiotropic effects on yield-related traits were analyzed. Spike compactness (SC) and length (SL) are greatly associated with wheat (Triticum aestivum L.) grain yield. To detect quantitative trait loci (QTL) associated with SC and SL, two biparental populations derived from crosses of Chuanmai42/Kechengmai1 and Chuanmai42/Chuannong16 were employed to perform QTL mapping in five environments. A total of 34 QTLs were identified, in which six major QTLs were repeatedly detected in more than four environments and the best linear unbiased prediction datasets, explaining 7.13-33.6% of phenotypic variation. These major QTLs were co-located in two genomic regions on chromosome 5A and 6A, namely QSc/Sl.cib-5A and QSc/Sl.cib-6A, respectively. By developing kompetitive allele-specific PCR (KASP) markers that linked to them, the two loci were validated in different genetic backgrounds, and their interactions were also analyzed. Comparison analysis showed that QSc/Sl.cib-5A was not Vrn-A1 and Q, and QSc/Sl.cib-6A was likely a new locus for SC and SL. Both QSc/Sl.cib-5A and QSc/Sl.cib-6A had pleiotropic effects on other yield-related traits including plant height, thousand grain weight and grain length. Therefore, the two loci combined with the developed KASP markers might be potentially applicable in wheat breeding. Furthermore, based on the spatiotemporal expression patterns, gene annotation, orthologous search and sequence differences, TraesCS5A01G301400 and TraesCS6A01G090300 were considered as potential candidates for QSc/Sl.cib-5A and QSc/Sl.cib-6A, respectively. These results provided valuable information for fine mapping and cloning of the two loci in the future.
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http://dx.doi.org/10.1007/s00122-021-03918-8DOI Listing
November 2021

Ground-State Structures of Hydrated Calcium Ion Clusters From Comprehensive Genetic Algorithm Search.

Front Chem 2021 30;9:637750. Epub 2021 Jun 30.

Key Laboratory of Materials Modification by Laser, Ion and Electron Beams, Ministry of Education, Dalian University of Technology, Dalian, China.

We searched the lowest-energy structures of hydrated calcium ion clusters Ca(HO) ( = 10-18) in the whole potential energy surface by the comprehensive genetic algorithm (CGA). The lowest-energy structures of Ca(HO) clusters show that Ca is always surrounded by six HO molecules in the first shell. The number of first-shell water molecules changes from six to eight at = 12. In the range of = 12-18, the number of first-shell water molecules fluctuates between seven and eight, meaning that the cluster could pack the water molecules in the outer shell even though the inner shell is not full. Meanwhile, the number of water molecules in the second shell and the total hydrogen bonds increase with an increase in the cluster size. The distance between Ca and the adjacent water molecules increases, while the average adjacent O-O distance decreases as the cluster size increases, indicating that the interaction between Ca and the adjacent water molecules becomes weaker and the interaction between water molecules becomes stronger. The interaction energy and natural bond orbital results show that the interaction between Ca and the water molecules is mainly derived from the interaction between Ca and the adjacent water molecules. The charge transfer from the lone pair electron orbital of adjacent oxygen atoms to the empty orbital of Ca plays a leading role in the interaction between Ca and water molecules.
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http://dx.doi.org/10.3389/fchem.2021.637750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277924PMC
June 2021

Six-photon upconverted excitation energy lock-in for ultraviolet-C enhancement.

Nat Commun 2021 07 16;12(1):4367. Epub 2021 Jul 16.

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.

Photon upconversion of near-infrared (NIR) irradiation into ultraviolet-C (UVC) emission offers many exciting opportunities for drug release in deep tissues, photodynamic therapy, solid-state lasing, energy storage, and photocatalysis. However, NIR-to-UVC upconversion remains a daunting challenge due to low quantum efficiency. Here, we report an unusual six-photon upconversion process in Gd/Tm-codoped nanoparticles following a heterogeneous core-multishell architecture. This design efficiently suppresses energy consumption induced by interior energy traps, maximizes cascade sensitizations of the NIR excitation, and promotes upconverted UVC emission from high-lying excited states. We realized the intense six-photon-upconverted UV emissions at 253 nm under 808 nm excitation. This work provides insight into mechanistic understanding of the upconversion process within the heterogeneous architecture, while offering exciting opportunities for developing nanoscale UVC emitters that can be remotely controlled through deep tissues upon NIR illumination.
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http://dx.doi.org/10.1038/s41467-021-24664-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285497PMC
July 2021

Barriers and facilitators to exercise in haemodialysis patients: A systematic review of qualitative studies.

J Adv Nurs 2021 Jul 14. Epub 2021 Jul 14.

Health Science Center, Xi'an Jiaotong University, Xi'an, Shaan Xi, China.

Aims: The purpose of this systematic review is to synthesize the results of qualitative research and to identify the barriers and facilitators to exercise in haemodialysis patients from the perspectives of haemodialysis patients, caregivers and dialysis staff members.

Design: Systematic review of qualitative studies.

Data Sources: Qualitative studies were extracted from MEDLINE, EMBASE, Web of Science, CINAHL, PsycINFO, PubMed, CBM, CNKI and WanFang Database from inception of each database until July 2020. Qualitative research and mixed method research including barriers and/or facilitators to exercise in haemodialysis patients were included.

Review Methods: The systematic search method SPIDER (sample, phenomenon of interest, design, evaluation, research type) was used. Thematic synthesis of qualitative data was used.

Results: 284 studies were screened and 10 studies published between 2007 and 2020 were finally included in this review. The review included 180 patients, 70 dialysis staff members and seven caregivers. Five analytical themes were identified: disease distress, perception of exercise, environmental restrictions, spirit strength and hospital management. Barriers include disease distress, perception of exercise (security issue), environmental restrictions and hospital management. Facilitators include perception of exercise (exercise being considered beneficial, preference for exercise) and spirit strength (from religious beliefs). It is the spiritual strength (from family and friends, from dialysis staff members) that is both the barrier and the facilitator.

Conclusion: There are many barriers in popularizing exercise among haemodialysis patients. Future intervention measures and health policies should strengthen the facilitators and reduce the barriers, so as to promote the clinical practice of exercise for haemodialysis patients.

Impact: This review summarizes the barriers and facilitators to exercise in haemodialysis patients. The results of this study have an impact on research, practice and health policy setting. The exercise level of haemodialysis patients can be improved by using the facilitators and solving the barriers.
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http://dx.doi.org/10.1111/jan.14960DOI Listing
July 2021

MiR-466 Inhibits the Progression of Severe Hepatocellular Carcinoma via Regulating FMNL2-Mediated Activation of NF-B and Wnt/-Catenin Pathways.

Authors:
Jianwei Li Su Yan

J Oncol 2021 23;2021:3554219. Epub 2021 Jun 23.

Department of Critical Care Medicine, Traditional Chinese Medicine Hospital of Rizhao City, Rizhao 276800, Shandong, China.

Hepatocellular carcinoma (HCC) has threatened the health of humans, and some evidence has indicated that miR-466 involves the progressions of some cancers. This study focused on the role of miR-466 in the formation and development of HCC. The expression levels of miR-466 in the tissues of patients and HCC cell lines were measured by qRT-PCR, and CCK-8, transwell assay, and flow cytometry assay were used to observe the functions of miR-466 on the HCC cells. Moreover, the miRNA databases, dual-luciferase reporter assay, and Western blot were used for the investigation of the regulation mechanism of miR-466 on HCC cells. The results showed that miR-466 was significantly downregulated in HCC tissues and cell lines, and inhibited proliferation, invasion, and high apoptosis were found in HCC cells when miR-466 was overexpressed. The results confirmed that FMNL2 was a target of miR-466, and increased FMNL2 could reverse the effects of miR-466 on the phenotype of HCC cells. Besides, it was also found that miR-466 was involved in the regulation of NF-B and Wnt/-catenin pathways in HCC cells via targeting FMNL2. In conclusion, the results of this study suggest that miR-466 regulates the activities of NF-B and Wnt/-catenin pathways to inhibit the progression of HCC cells via targeting FMNL2.
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http://dx.doi.org/10.1155/2021/3554219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249156PMC
June 2021

Integrating Reasoned Action Approach and Message Sidedness in the Era of Misinformation: The Case of HPV Vaccination Promotion.

Authors:
Xizhu Xiao Yan Su

J Health Commun 2021 06 10;26(6):371-380. Epub 2021 Jul 10.

The Edward R. Murrow College of Communication, Washington State University, Pullman, WA.

Building upon extant research on the reasoned action approach and message sidedness, this study investigates the persuasive effects of one-sided and two-sided social media messages on the attitude about human papillomavirus (HPV) vaccination in the context of misinformation. Results of a controlled experiment ( = 251) indicated that compared to the control, one-sided messages addressing misinformation increased positive attitude about the vaccine as prior misperceptions increased. However, a backfire effect may be looming for individuals with lower prior misperceptions. Within the sidedness conditions, refutational two-sided messages were more effective in increasing cognitive attitude for individuals with lower misperceptions; whereas one-sided messages had a persuasive advantage for individuals with higher misperceptions. Theoretical and practical contributions are discussed.
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http://dx.doi.org/10.1080/10810730.2021.1950873DOI Listing
June 2021

Phase Diagrams for sII Clathrate Hydrates of CO from First-Principles Thermodynamics.

J Phys Chem A 2021 Jul 6;125(27):5956-5962. Epub 2021 Jul 6.

Key Laboratory of Materials Modification by Laser, Ion and Electron Beams, Dalian University of Technology, Ministry of Education, Dalian 116024, China.

Clathrate hydrates are crystalline solid compounds consisting of a water caged framework and guest molecules such as CH, CH, and CO. Understanding the phase equilibrium conditions of hydrates is significantly important for the industrial exploitation and experimental synthesis of hydrates. Based on the correct description of the intermolecular noncovalent interactions of clathrate hydrates with vdW-DF2, we studied the crystal structures and the chemical potential phase diagrams of sII hydrates encapsulated with CO molecules to provide a deep understanding of the stability mechanism of hydrates. Under the given conditions, the partially occupied hydrates (136HO·1CO and 136HO·16CO) and fully occupied hydrates (136HO·24CO) are thermodynamically stable, and the equilibrium temperature decreases as the relative CO chemical potential increases at the same pressure. We expect that the present study may provide vital information on the stability conditions of CO hydrates and trigger new experiments to establish an effective replacement strategy for CO/CH.
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http://dx.doi.org/10.1021/acs.jpca.1c04673DOI Listing
July 2021
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