Publications by authors named "Yan Shi"

1,713 Publications

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Investigation of the crosstalk between GRP78/PERK/ATF-4 signaling pathway and renal apoptosis induced by nephropathogenic infectious bronchitis virus infection.

J Virol 2021 Oct 20:JVI0142921. Epub 2021 Oct 20.

Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, PR China.

This study aims to explore the crosstalk between GRP78/PERK/ATF-4 signaling pathway and renal apoptosis induced by nephropathogenic infectious bronchitis virus (NIBV). Hy-Line brown chickens were divided into two groups (Con, n=100 and Dis, n=200). At 28 days of age, each chicken in the Dis group was intranasally injected with SX9 strain (10/0.2ml). Venous blood and kidney tissues were collected at 1, 5, 11, 18 and 28 days post infection. Our results showed that NIBV infection upregulated the levels of creatinine, uric acid and calcium (Ca) levels. Histopathological examination revealed severe hemorrhage and inflammatory cell infiltration near the renal tubules. Meanwhile, NIBV virus particles and apoptotic bodies were observed by ultramicro electron microscope. In addition, RT-qPCR and Western blot showed that NIBV up-regulated the expression of GRP78, PERK, eIF2α, ATF-4, CHOP, Caspase-3, Caspase-9, P53, Bax, and on the contrary, down-regulated the expression of Bcl-2. Furthermore, immunofluorescence localization analysis showed that the positive expression of Bcl-2 protein was significantly decreased. Correlation analysis indicated that endoplasmic reticulum (ER) stress gene expression, apoptosis gene expression and renal injury were potentially related. Taken together, NIBV infection can induce renal ER stress and apoptosis by activating of GRP78/PERK/ATF-4 signaling pathway, leading to kidney damage. Nephropathogenic infectious bronchitis virus (NIBV) induced renal endoplasmic reticulum stress in chickens. NIBV infection induced kidney apoptosis in chickens. GRP78/PERK/ATF-4 signaling pathway is potentially related to renal apoptosis induced by NIBV.
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http://dx.doi.org/10.1128/JVI.01429-21DOI Listing
October 2021

Sandalwood seed oil improves insulin sensitivity in high-fat/high-sucrose diet-fed rats associated with altered intestinal microbiota and its metabolites.

Food Funct 2021 Oct 19;12(20):9739-9749. Epub 2021 Oct 19.

Institute of Nutrition & Health, College of Public Health, Qingdao University, Qingdao, China.

Sandalwood seed oil (SSO), rich in ximenynic acid, is extracted from the seed kernels of . The current work aimed to clarify the potential mechanisms of SSO in preventing insulin resistance (IR) by investigating the intestinal microbiota and its metabolites. Fifty male Sprague-Dawley rats were randomly divided into a standard chow group (N), and four high-fat/high-sucrose (HFHS) diet-fed groups plus 7% of SSO, fish oil (FO), linseed oil (LO) or sunflower oil (SO), respectively. After 12 weeks, the feces were collected and subsequently the rats were sacrificed for collecting blood and tissues. The results indicated that the SSO, FO and LO groups had a lower ratio of Firmicutes to Bacteroidetes (F/B) and lower levels of Actinobacteria phylum in their feces compared to the SO group. HOMA-IR was positively correlated with F/B ( = 0.63) and Actinobacteria ( = 0.64). At the genus level, beneficial bacteria, including , , , and , were more abundant, while destructive bacteria, such as , were less abundant in the SSO group than in the SO group. The concentrations of fecal short-chain fatty acids (SCFAs) were higher, and the serum LPS and trimethylamine -oxide (TMAO) were lower in the SSO, FO and LO groups than the SO group. In addition, SCFAs were negatively (: -0.45 to -0.82), and LPS (: 0.12 to 0.42) and TMAO (: 0.32 to 0.49) were positively correlated with HOMA-IR and serum IL-1β, IL-6 and TNF-α. In summary, the prevention effect of SSO on HFHS induced IR was associated with altered intestinal microbiota composition and the production of microbial metabolites.
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http://dx.doi.org/10.1039/d1fo02239cDOI Listing
October 2021

The Research Progress of Electrical Impedance Tomography for Lung Monitoring.

Front Bioeng Biotechnol 2021 1;9:726652. Epub 2021 Oct 1.

The School of Automation Science and Electrical Engineering, Beihang University, Beijing, China.

Medical imaging can intuitively show people the internal structure, morphological information, and organ functions of the organism, which is one of the most important inspection methods in clinical medical diagnosis. Currently used medical imaging methods can only be applied to some diagnostic occasions after qualitative lesions have been generated, and the general imaging technology is usually accompanied by radiation and other conditions. However, electrical impedance tomography has the advantages of being noninvasive and non-radiative. EIT (Electrical Impedance Tomography) is also widely used in the early diagnosis and treatment of some diseases because of these advantages. At present, EIT is relatively mature and more and more image reconstruction algorithms are used to improve imaging resolution. Hardware technology is also developing rapidly, and the accuracy of data collection and processing is continuously improving. In terms of clinical application, EIT has also been used for pathological treatment of lungs, the brain, and the bladder. In the future, EIT has a good application prospect in the medical field, which can meet the needs of real-time, long-term monitoring and early diagnosis. Aiming at the application of EIT in the treatment of lung pathology, this article reviews the research progress of EIT, image reconstruction algorithms, hardware system design, and clinical applications used in the treatment of lung diseases. Through the research and introduction of several core components of EIT technology, it clarifies the characteristics of EIT system complexity and its solutions, provides research ideas for subsequent research, and once again verifies the broad development prospects of EIT technology in the future.
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http://dx.doi.org/10.3389/fbioe.2021.726652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517404PMC
October 2021

Short-Term Clinical Efficacy of Neoadjuvant Chemotherapy Combined With Laparoscopic Gastrectomy for Locally Advanced Siewert Type II and III Adenocarcinoma of the Esophagogastric Junction: A Retrospective, Propensity Score-Matched Study.

Front Oncol 2021 29;11:690662. Epub 2021 Sep 29.

Department of General Surgery, The First Affiliated Hospital, Army Medical University, Chongqing, China.

Background: Laparoscopic gastrectomy (LG) has been increasingly used for the treatment of locally advanced Siewert type II and III adenocarcinoma of the esophagogastric junction (AEG). However, whether LG can achieve the same short-term efficacy in the treatment of patients who receive neoadjuvant chemotherapy (NACT) remains controversial. Thus, the aim of this study was to investigate the clinical outcomes of NACT combined with LG for Siewert type II and III AEG.

Methods: This retrospective study identified patients with locally advanced Siewert type II and III AEG diagnosed between May 2011 and October 2020 using the clinical tumor-node-metastasis (cTNM) staging system. The short-term outcomes were compared between the matched groups using a 1:3 propensity score matching (PSM) method, which was performed to reduce bias in patient selection.

Results: After PSM, 164 patients were selected, including 41 in the NACT group and 123 in the LG group. The baseline characteristics were similar between the two groups. Compared with the LG group, the NACT group exhibit a smaller tumor size and significantly less advanced pathological tumor classification and nodal classification stages. The time to first flatus of the NACT group was significantly shorter, but the hospital stay was significantly longer than that of the LG group. The NACT group showed similar overall (29.3% 25.2%, P=0.683), systemic (24.4% 21.1%, P=0.663), local (12.2% 9.8%, P=0.767), minor (19.5% 19.5%, P=1.000) and major (9.8% 5.7%, P=0.470) complications as the LG group. Subgroup analyses showed no significant differences in most stratified parameters. Operation time≥ 300 minutes was identified as an independent risk factor for overall complications. Age≥ 60 years was identified as an independent risk factor for major complications.

Conclusion: NACT combined with LG for AEG does not increase the risk of postoperative morbidity and mortality compared with LG.
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http://dx.doi.org/10.3389/fonc.2021.690662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511681PMC
September 2021

Insight into the Different Channel Proteins of Human Red Blood Cell Membranes Revealed by Combined dSTORM and AFM Techniques.

Anal Chem 2021 Oct 17. Epub 2021 Oct 17.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China.

Membrane proteins tend to interact with each other in the cell membranes to form protein clusters and perform the corresponding physiological functions. However, because channel proteins are involved in many biological functions, their distribution and nano-organization in these protein clusters are unclear. To study the distribution patterns and relationships between the different channel proteins, we identified the locations of glucose transporter 1 (Glut1) and Band3 (anion transporter 1) precisely in the topography of the cytoplasmic side of the human red blood cell (hRBC) membranes using combined atomic force microscopy (AFM) and single-molecule localization microscopy (SMLM). The AFM results revealed that membrane proteins interacted with each other and aggregated into protein islands. The SMLM results showed that Glut1 and Band3 tended to form protein clusters in the hRBC membranes, and there was a strong colocalization between the two proteins. The results of the combined AFM and SMLM method indicated that the protein clusters of Glut1 and Band3 were mainly located in the protein islands of topography, and the protein islands in topography also interacted with each other to assemble into larger protein clusters or functional microdomains.
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http://dx.doi.org/10.1021/acs.analchem.1c02382DOI Listing
October 2021

Synthesis of Indole-Fused Oxepines via C-H Activation Initiated Diastereoselective [5 + 2] Annulation of Indoles with 1,6-Enynes.

Org Lett 2021 Oct 15. Epub 2021 Oct 15.

Department of Material Chemistry, School of Chemistry and Xi'an Key Laboratory of Sustainable Energy Materials Chemistry, Xi'an Jiaotong University, Xi'an 710049, P. R. China.

A rhodium-catalyzed diastereoselective formal [5 + 2] annulation of indoles with cyclohexadienone-containing 1,6-enynes has been established via indole 2,3-difunctionalization. The reaction, probably proceeding through tandem indole C2-H alkenylation and intramolecular Friedel-Crafts alkylation relay, provides rapid construction of indole-fused oxepines in good to excellent yields with a broad substrate scope. This method also features concomitant construction of -hydrobenzo[] oxepine scaffolds, a core unit found in numerous natural products of important biological activities.
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http://dx.doi.org/10.1021/acs.orglett.1c03106DOI Listing
October 2021

XRCC2 Arg188His polymorphism and colorectal cancer risk: a meta-analysis.

J Int Med Res 2021 Oct;49(10):3000605211039473

Department of Medical Oncology, Affiliated Hospital of Hebei University, Baoding, Hebei Province, China.

Objective: To investigate the potential correlation between the Arg188His (rs3218536) polymorphism of X-ray repair cross-complementing 2 () and colorectal cancer (CRC) risk, as the association remains unclear.

Methods: The CNKI, PubMed, EMBASE and Cochrane library databases were systematically searched for relevant studies published up to July 2021. Data were extracted from included studies, and analysed for pooled or subgroup odds ratios (ORs) with 95% confidence intervals (CIs) using STATA 12.0 software.

Results: Seven published studies were included. Pooled analysis revealed that the Arg188His polymorphism was associated with increased CRC risk (His versus Arg: OR 1.14, 95% CI 1.01, 1.29). Trial Sequential Analysis to test the power of the results showed that they were unreliable and the meta-analysis required additional studies.

Conclusion: The current meta-analysis suggests that the Arg188His polymorphism may be a risk factor for CRC.
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http://dx.doi.org/10.1177/03000605211039473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521768PMC
October 2021

Expression and function analysis of CRABP2 and FABP5, and their ratio in esophageal squamous cell carcinoma.

Open Med (Wars) 2021 27;16(1):1444-1458. Epub 2021 Sep 27.

Departments of Pathology, The First Affiliated Hospital, Xinjiang Medical University, 393 Liyushan Road, Urumqi, Xinjiang 830011, People's Republic of China.

Objective: The purpose of this study was to explore the effect of CRABP2 and FABP5, and their ratio on prognosis in esophageal squamous cell carcinoma.

Methods: The expression data of CRABP2 in esophageal cancer in TCGA and GEO were collected by the public database GEPIA. The expression levels of CRABP2 and FABP5 were examined using immunohistochemistry. The relationship between the two proteins and related clinicopathological parameters were analyzed by test. Survival analysis was used to investigate the effect of CRABP2 and FABP5, and their ratio on prognosis.

Results: Compared with normal esophageal mucosal epithelium, there was lower CRABP2 gene mRNA in the esophageal cancer tissue, and the difference was statistically significant ( < 0.01). For the expression level, no significant difference was observed in patients with stages I-IV in esophageal cancer. Immunohistochemistry showed that CRABP2 and FABP5 were both highly expressed in normal esophageal squamous epithelial cells at 100 and 94.1%, while lower in ESCC (75.6 and 58.7%). There was a significant difference in the expression between cancer and adjacent tissues ( < 0.001). No inherent relationship was manifested between the CRABP2 expression and the clinical parameters of the ESCC. The expression of FABP5 was related to lymph node metastasis ( = 0.032), the depth of invasion ( = 0.041), and the AJCC stage ( = 0.013). The ratio of CRABP2 and FABP5 was related to ethnicity ( = 0.001), nerve invasion ( = 0.031), and postoperative treatment ( = 0.038). CRABP2 is positively associated with FABP5 ( = 0.156, = 0.041) and the ratio ( = 0.334, = 0.000), while there was a negative correlation between FABP5 and the ratio ( = -0.269, = 0.000). Patients with CRABP2-positive expression had a significantly longer overall survival than patients with CRABP2-negative expression ( = 0.025).

Conclusion: CRABP2 as a suppressor factor is expected to be a potential prognosis marker for esophageal squamous cell carcinoma.
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http://dx.doi.org/10.1515/med-2021-0350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477672PMC
September 2021

Editorial: The Role of Biomembranes and Biophysics in Immune Cell Signaling.

Front Immunol 2021 24;12:740373. Epub 2021 Sep 24.

Department of Cell Biology and Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

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http://dx.doi.org/10.3389/fimmu.2021.740373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498022PMC
September 2021

Nickel clusters accelerating hierarchical zinc indium sulfide nanoflowers for unprecedented visible-light hydrogen production.

J Colloid Interface Sci 2021 Sep 29;608(Pt 1):504-512. Epub 2021 Sep 29.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, 122 Luoshi Road, 430070 Wuhan, Hubei, China; NRC (Nanostructure Research Centre), Wuhan University of Technology, 122 Luoshi Road, 430070 Wuhan, Hubei, China. Electronic address:

As a typical two-dimensional (2D) metal chalcogenides and visible-light responsive semiconductor, zinc indium sulfide (ZnInS) has attracted much attention in photocatalysis. However, the high recombination rate of photogenerated electrons and holes seriously limits its performance for hydrogen production. In this work, we report in-situ photodeposition of Ni clusters in hierarchical ZnInS nanoflowers (Ni/ZnInS) to achieve unprecedented photocatalytic hydrogen production. The Ni clusters not only provide plenty of active sites for reactions as evidenced by in-situ photoluminescence measurement, but also effectively accelerate the separation and migration of the photogenerated electrons and holes in ZnInS. Consequently, the Ni/ZnInS composites exhibit good stability and reusability with highly enhanced visible-light hydrogen production. In particular, the best Ni/ZnInS photocatalyst exhibits an unprecedented hydrogen production rate of 22.2 mmol·h·g, 10.6 times that of the pure ZnInS (2.1 mmol·h·g). And its apparent quantum yield (AQY) is as high as 56.14% under 450 nm monochromatic light. Our work here suggests that depositing non-precious Ni clusters in ZnInS is quite promising for the potential practical photocatalysis in solar energy conversion.
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http://dx.doi.org/10.1016/j.jcis.2021.09.156DOI Listing
September 2021

A new calnexin modulates antibacterial immune response in obscure puffer Takifugu obscurus.

Dev Comp Immunol 2021 Oct 5;127:104288. Epub 2021 Oct 5.

College of Oceanography, Hohai University, 1 Xikang Road, Nanjing, Jiangsu, 210098, China. Electronic address:

Calnexin (Cnx) is a membrane-bound lectin chaperone of the endoplasmic reticulum. In this study, a novel Cnx homologue from the obscure puffer Takifugu obscurus was characterized, tentatively named ToCnx. The cDNA of ToCnx was 1803 bp, and it contained an open reading frame encoding a polypeptide of 600 amino acid residues with a calculated molecular weight of 67.5 kDa. Multiple alignment of the deduced amino acid sequences of ToCnx and other related fish Cnxs revealed that ToCnx had typical characteristics of fish Cnxs. Sequence comparison and phylogenetic tree analysis showed that ToCnx had the closest relationship with Cnxs from Takifugu flavidus and Takifugu rubripes. ToCnx transcripts were detected in all the tissues examined, and they were mainly expressed in the liver, kidney, and intestine. Upon Vibrio harveyi, Edwardsiella tarda, and Aeromonas hydrophila infection, ToCnx transcripts were all significantly upregulated in the kidneys. The recombinant calreticulin domain of ToCnx (rToCnx) was prepared by prokaryotic expression. In the absence of calcium, rToCnx was able to bind three Gram-negative bacteria (V. harveyi, E. tarda, and A. hydrophila) and two bacterial saccharides, such as lipopolysaccharide and peptidoglycan. In the presence of calcium, rToCnx could agglutinate all the detected microorganisms. In addition, rToCnx possessed the effect of inhibiting the growth of three microbe strains. These observations suggested that ToCnx is an important participant in host immune defense against bacteria.
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http://dx.doi.org/10.1016/j.dci.2021.104288DOI Listing
October 2021

Quantitative analysis of early diabetic retinopathy based on optical coherence tomography angiography biological image.

World J Clin Cases 2021 Sep;9(25):7365-7371

Department of Ophthalmology, Ningbo First Hospital, Ningbo 315000, Zhejiang Province, China.

Background: With the development of the economy and improvements in living standards, the incidences of diabetes mellitus (DM) and diabetic retinopathy (DR), which is a complication of DM, are on the rise.

Aim: To analyze early DR in patients with macular zone changes in biological images using optical coherence tomography angiography.

Methods: A prospective case study was performed on 59 participants: 35 healthy eyes (control group), 35 eyes with diabetes but no DR group (no DR group), and 35 eyes with mild DR (NPDR group). All quantitative comparisons of parameters, including the fovea vascularity area, circularity index, and vascular complexity parameters, were performed using a biological image analysis software.

Results: The foveal avascular zone (FAZ) area, FAZ circularity index, number of branches in the area, and the total of the single branches' length in the area was 0.366 ± 0.031, 0.834 ± 0.037, 3241.8 ± 268.3, and 3.860 × 10 ± 0.194 × 10, and 0.421 ± 0.030, 0.739 ± 0.023, 2956.6 ± 476.4, and 3.177 × 10 ± 0.161 × 10 in the no DR group and the NPDR group, respectively, which were significantly different from the corresponding parameters of the control group ( < 0.05). Moreover, there were significant differences between these two groups ( < 0.05).

Conclusion: This study shows that early microcirculation changes in the macular area of the retina is associated with disease progression. Early changes in DR can be analyzed using optical coherence tomography angiography.
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http://dx.doi.org/10.12998/wjcc.v9.i25.7365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464476PMC
September 2021

N-3 polyunsaturated fatty acids effectively protect against neural tube defects in diabetic mice induced by streptozotocin.

Food Funct 2021 Oct 4;12(19):9188-9196. Epub 2021 Oct 4.

Institute of Nutrition and Health, Qingdao University, China.

Folate cannot prevent all neural tube defects (NTD), indicating that other pathogeneses still exist except for the folate deficiency. Maternal diabetes mellitus during pregnancy can increase the risk of offspring NTD. Our previous study showed that polyunsaturated fatty acids (PUFA) were lower in the placenta of human NTD cases than in healthy controls, and the supplementation of fish oil (rich in long-chain (LC) n-3 PUFA, mainly C20:5n-3 and C22:6n-3) had a better prevention effect against sodium valproate induced NTD than corn oil (rich in C18:2n-6) and flaxseed oil (rich in C18:3n-3). The aim of the present study was to investigate whether PUFA could prevent diabetes-induced NTD in mice. Streptozotocin (STZ)-induced diabetic pregnant mice were fed with a normal diet (DMC), a diet containing a low dose of fish oil (DMLn-3), a diet containing a high dose of fish oil (DMHn-3) or a diet rich in corn oil (DMn-6). Healthy pregnant mice were fed with a normal diet (HC). Compared with the DMC group, the rate of NTD was significantly lower in the DMHn-3 group (4.44% 12.50%), but not in the DMLn-3 (11.11%) or DMn-6 group (12.03%). The NTD rate in the DMHn-3 group was comparable with that in the HC group (1.33%) ( = 0.246), and lower than that in the DMn-6 group ( = 0.052). The NTD rate in DMLn-3 and DMn-6 groups was significantly higher than that in the HC group. No significant difference was observed in NTD rate between DMLn-3 and DMHn-3 groups, and between DMLn-3 and DMn-6 groups. Compared with the HC group, the DMC group had a significantly lower C22:6n-3 in both serum and embryos. Fish oil supplementation ameliorated neuroepithelial cell apoptosis, and the apoptotic rate was comparable between DMHn-3 and HC groups. Although the apoptotic rate was significantly lower in the DMn-6 group than the DMC group, it was still much higher than that in the HC group. The proteins P53 and Bax in embryos were higher, while the proteins Bcl-2 and Pax3 were lower in the DMC group than in the HC group. The disturbance of Pax3, P53 and Bax induced by diabetes was abolished in DMLn-3, DMHn-3 and DMn-6 groups. Importantly, Bcl-2 in embryos was restored to the normal level only in the DMHn-3 group but not in the DMLn-3 or DMn-6 group. In conclusion, LC n-3 PUFA enriched fish oil has a protective effect against NTD in diabetes induced by STZ through improving neuroepithelial cell apoptosis, and the mechanism may be by increasing the anti-apoptosis protein Bcl-2 independently of Pax3 and P53.
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http://dx.doi.org/10.1039/d1fo01606gDOI Listing
October 2021

40 Hz Light Flicker Promotes Learning and Memory via Long Term Depression in Wild-Type Mice.

J Alzheimers Dis 2021 Sep 30. Epub 2021 Sep 30.

The Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences; Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen, China.

Background: 40 Hz light flicker is a well-known non-invasive treatment that is thought to be effective in treating Alzheimer's disease. However, the effects of 40 Hz visual stimulation on neural networks, synaptic plasticity, and learning and memory in wild-type animals remain unclear.

Objective: We aimed to explore the impact of 40 Hz visual stimulation on synaptic plasticity, place cell, and learning and memory in wild-type mice.

Methods: c-Fos+ cell distribution and in vivo electrophysiology was used to explore the effects of 40 Hz chronic visual stimulation on neural networks and neuroplasticity in wild-type mice. The character of c-Fos+ distribution in the brain and the changes of corticosterone levels in the blood were used to investigate the state of animal. Place cell analysis and novel location test were utilized to examine the effects of 40 Hz chronic visual stimulation on learning and memory in wild-type mice.

Results: We found that 40 Hz light flicker significantly affected many brain regions that are related to stress. Also, 40 Hz induced gamma enrichment within 15 min after light flickers and impaired the expression of long-term potentiation (LTP), while facilitated the expression of long-term depression (LTD) in the hippocampal CA1. Furthermore, 40 Hz light flicker enhanced the expression of corticosterone, rendered well-formed place cells unstable and improved animal's learning and memory in novel local recognition test, which could be blocked by pre-treatment with the LTD specific blocker Glu2A-3Y.

Conclusion: These finding suggested that 40 Hz chronic light flicker contains stress effects, promoting learning and memory in wild-type mice via LTD.
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http://dx.doi.org/10.3233/JAD-215212DOI Listing
September 2021

Transcriptome profiling revealed the growth superiority of hybrid pufferfish derived from Takifugu obscurus ♀ × Takifugu rubripes ♂.

Comp Biochem Physiol Part D Genomics Proteomics 2021 Sep 29;40:100912. Epub 2021 Sep 29.

Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, China. Electronic address:

Hybridization is an efficient method to breed new strains of aquatic animals. In the present study, we produced a hybrid puffer by crossing female obscure puffer with male tiger puffer. The hybrid puffer could live in fresh water like obscure puffer and exhibited growth superiority. The averaged body weight of 4- and 6-month-old hybrid puffer were respectively 38.06% and 38.93% higher than that of obscure puffer. Then, we analyzed the underlying genetic basis for the growth advantage of hybrid puffer by comparative transcriptome analysis. A total number of 4264 and 1285 differentially expressed genes (DEGs) were respectively identified from pituitary and liver transcriptome profiles between hybrid puffer and obscure puffer. Comprehensive analysis showed that the DEGs related with cell proliferation and differentiation, and protein synthesis and export, specifically showed higher expression levels in hybrid puffer, such as "ECM-receptor interaction", "focal adhesion", "protein export" and "protein processing in endoplasmic reticulum". While the DEGs involved in gametogenesis and carbohydrate and energy metabolism highly expressed in obscure puffer, such as "oxidative phosphorylation", "citrate cycle", "progesterone-mediated oocyte maturation" and "oocyte meiosis". Furthermore, a series of candidate genes related to the growth superiority of hybrid puffer were identified, such as fn1a, ptprc, plcg2, igf1, tgfβ1, bmp4, abl1, col1a2, col1a1a, and myl9a. These results will be beneficial to understand the molecular basis of growth superiority and helpful to the hybrid breeding of pufferfish.
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http://dx.doi.org/10.1016/j.cbd.2021.100912DOI Listing
September 2021

Author Correction: Accumulation of formaldehyde causes motor deficits in an in vivo model of hindlimb unloading.

Commun Biol 2021 Oct 1;4(1):1160. Epub 2021 Oct 1.

Beijing Institute of Brain Disorders, Laboratory of Brain Disorders, Ministry of Science and Technology, Collaborative Innovation Center for Brain Disorders, Capital Medical University, Beijing, China.

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http://dx.doi.org/10.1038/s42003-021-02713-xDOI Listing
October 2021

Author Correction: MDM4 inhibition: a novel therapeutic strategy to reactivate p53 in hepatoblastoma.

Sci Rep 2021 Oct 1;11(1):19916. Epub 2021 Oct 1.

Divisions of Pediatric Surgery and Surgical Research, Michael E. DeBakey Department of Surgery, Pediatric Surgical Oncology Laboratory, Texas Children's Surgical Oncology Program, Texas Children's Liver Tumor Program, Dan L. Duncan Cancer Center, Baylor College of Medicine, Texas Children's Hospital, 1102 Bates Ave., Suite 460G, Houston, TX, 77030-2399, USA.

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http://dx.doi.org/10.1038/s41598-021-98174-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486835PMC
October 2021

Systematic comparisons of dissolving and swelling hyaluronic acid microneedles in transdermal drug delivery.

Int J Biol Macromol 2021 Sep 28;191:783-791. Epub 2021 Sep 28.

College of Marine Life Science, Ocean University of China, 5 Yushan Road, 266003 Qingdao, China. Electronic address:

Transdermal drug delivery efficacy of hyaluronic acid dissolving microneedles (HA DMNs) is limited by low loading dose and poor drug condensation in needles. HA swelling MNs (HA SMNs) could improve the efficacy, but comparisons between the formulations is missing. In this work, DMNs and SMNs were fabricated of HA and methacrylated HA, respectively. Their properties of transdermal drug delivery were systematically compared. HA SMNs exhibited enhanced mechanical strength, fast swelling performance, and extended duration of microchannels in skin. The doxorubicin (DOX) loaded by one-step loading protocol in needles and baseplate of SMNs could be transdermally delivered through the diffusing path mediated by swollen needles, conquering the limit of poor drug condensation in DMNs needles and generated a longer Tmax but higher Cmax. The relative bioavailability of DOX/SMNs towards DOX/DMNs was 200% within 12 h. The results provide theoretical references for the application of HA MNs mediated transdermal drug delivery.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.09.161DOI Listing
September 2021

Activity waves and freestanding vortices in populations of subcritical Quincke rollers.

Proc Natl Acad Sci U S A 2021 Oct;118(40)

Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou 215006, China;

Virtually all of the many active matter systems studied so far are made of units (biofilaments, cells, colloidal particles, robots, animals, etc.) that move even when they are alone or isolated. Their collective properties continue to fascinate, and we now understand better how they are unique to the bulk transduction of energy into work. Here we demonstrate that systems in which isolated but potentially active particles do not move can exhibit specific and remarkable collective properties. Combining experiments, theory, and numerical simulations, we show that such subcritical active matter can be realized with Quincke rollers, that is, dielectric colloidal particles immersed in a conducting fluid subjected to a vertical DC electric field. Working below the threshold field value marking the onset of motion for a single colloid, we find fast activity waves, reminiscent of excitable systems, and stable, arbitrarily large self-standing vortices made of thousands of particles moving at the same speed. Our theoretical model accounts for these phenomena and shows how they can arise in the absence of confining boundaries and individual chirality. We argue that our findings imply that a faithful description of the collective properties of Quincke rollers need to consider the fluid surrounding particles.
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http://dx.doi.org/10.1073/pnas.2104724118DOI Listing
October 2021

[Identification of geographical origins of Cordyceps based on data of amino acids with self-organizing map neural network].

Zhongguo Zhong Yao Za Zhi 2021 Sep;46(18):4765-4773

Traditional Chinese Medicine Quality Evaluation of the Key Laboratory of Beijing, Beijing University of Chinese Medicine Beijing 102488, China.

In this study, data of amino acids of Cordyceps samples from Qinghai and Tibet was analyzed with self-organizing map neural network. A model of XY-Fused network was established with the content of 8 major amino acids and total amino acids for the identification of geographical origins of Cordyceps from Qinghai and Tibet. It had the prediction accuracy of 83.3% for the test set. In addition, data mining indicated that methionine was a special kind of amino acid in Cordyceps which could serve as a marker to identify its geographical origins. On this basis, the content ratio of methionine to total amino acids was proposed to be a quantifiable indicator to distinguish Cordyceps from Qinghai and Tibet.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210623.203DOI Listing
September 2021

Optimization of the Preparation Parameters of High-Strength Nickel Layers by Electrodeposition on Mild Steel Substrates.

Materials (Basel) 2021 Sep 21;14(18). Epub 2021 Sep 21.

Department of Mechanics, Tianjin University of Commerce, Tianjin 300134, China.

The electrodeposition process parameters were optimized for the acquisition of high-strength monolithic nickel layers on Q235A substrates based on the Watts nickel plating solution using the DC electrodeposition method. Based on the study of the electrochemical polarization behavior of nickel ions in Watts' plating solution, 16 experimental protocols were selected according to the orthogonal test method. The residual stress, microhardness, modulus of elasticity, and surface roughness of the nickel plating were tested by X-ray diffractometer, nano-mechanical test system, and surface profilometer, respectively, to investigate the influence of current density, temperature, and PH on the mechanical properties of nickel plating, so as to determine the best process solution for the preparation of high-strength nickel plating. The results of the study show that the mechanical properties of the nickel deposits electrodeposited onto Q235A are optimized when plating at a current density of 3 A/dm, a bath temperature of 45 °C, and a pH of 3.5. The nickel-plated layer has a minimum grain size of 34.8 nm, a microhardness of 3.86 GPa, a modulus of elasticity of 238 GPa, and a surface roughness Ra of 0.182 μm.
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http://dx.doi.org/10.3390/ma14185461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467751PMC
September 2021

Effects of Subchronic Copper Poisoning on Cecal Histology and Its Microflora in Chickens.

Front Microbiol 2021 8;12:739577. Epub 2021 Sep 8.

Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.

Copper (Cu) is an important trace element with a two-sided effect on the growth performance of animals, which depends on the timing and dosage of Cu addition, etc. The purpose of this study was to determine the effects of oral copper sulfate (CuSO, 350 ppm) on growth performance, cecal morphology, and its microflora of chickens ( = 60) after 30, 60, and 90 days. The results showed that after 90 days of copper exposure, the chickens lost weight, the cecum mucosa was detached, and vacuolation and inflammatory infiltration occurred at the base of the lamina propria. In addition, using the 16S rDNA sequencing method, we observed that copper exposure changed the richness and diversity of intestinal microorganisms. At the phylum level, and both significantly increased, while significantly decreased in the Cu group compared with control check (CK) group. At the genus level, the relative abundance of decreased significantly, while , and increased significantly after copper exposure, and the change in microflora was most significant at 90 days. Moreover, the relevance of genus-level bacteria was altered. PICRUST analysis revealed potential metabolic changes associated with copper exposure, such as infection and metabolic disorders of nutrients. To sum up, these data show that subchronic copper exposure not only affects the growth and development of chickens but also causes the imbalance of intestinal microflora, which may further induce metabolic disorders in chickens.
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http://dx.doi.org/10.3389/fmicb.2021.739577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456085PMC
September 2021

Comparison of the Postoperative Complications Between Robotic Total and Distal Gastrectomies for Gastric Cancer Using Clavien-Dindo Classification: A Propensity Score-matched Retrospective Cohort Study of 726 Patients.

Surg Innov 2021 Sep 22:15533506211047011. Epub 2021 Sep 22.

Department of General Surgery, Center for Minimally Invasive Gastrointestinal Surgery, Southwest Hospital, 12525Third Military Medical University, Chongqing, China.

Background: This study was designed to compare the postoperative complications after Robotic total gastrectomy (RTG) and robotic distal gastrectomy (RDG) and to systematically evaluate the safety and feasibility of RTG for the treatment of gastric cancer (GC).

Methods: Patients with GC who underwent RTG or RDG for curative intent between March 2010 and August 2019 were analyzed. We used propensity score matching (PSM) to reduce selection bias. The morbidity and mortality within 30 days after surgery between the RTG and the RDG groups were compared.

Results: According to Clavien-Dindo (C-D) classification, the morbidity and mortality of the RTG group were comparable to those of the RDG group. Subgroup analyses showed no significant difference between the RTG and RDG groups in all stratified parameters (all > .05). Multivariate analysis revealed that age ≥70 years ( = .002) and surgeons' experience ≤25 cases ( = .013) were independent risk factors for overall complication. Surgeons' experience ≤25 cases ( = .010) was identified as an independent risk factor for severe complication.

Conclusion: RTG is a safe and feasible surgical procedure for the treatment of GC with acceptable morbidity and mortality. More complications were observed for RTG, indicating that RTG is more invasive than RDG.
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http://dx.doi.org/10.1177/15533506211047011DOI Listing
September 2021

Identification of Differentially Expressed Circular RNAs as miRNA Sponges in Lung Adenocarcinoma.

J Oncol 2021 10;2021:5193913. Epub 2021 Sep 10.

The Fourth Department of Medical Oncology, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin 150040, China.

Background: Circular RNAs (circRNAs) may function as the decoys for microRNAs (miRNAs) or proteins, the templates for translation, and the sources of pseudogene generation. The purpose of this study is to determine the diagnostic circRNAs, which are related to lung adenocarcinoma (LUAD), that adsorb miRNAs on the basis of the competing endogenous RNA (ceRNA) hypothesis.

Methods: The differentially expressed circRNAs (DEcircRNAs) in LUAD were revealed by the microarray data (GSE101586 and GSE101684) that were obtained from the Gene Expression Omnibus (GEO) database. The miRNAs that were targeted by the DEcircRNAs were predicted with the CircInteractome, and the target mRNAs of the miRNAs were found by the miRDB and the TargetScan. The ceRNA network was built by the Cytoscape. The potential biological roles and the regulatory mechanisms of the circRNAs were investigated by the Gene Ontology (GO) enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The expression of the host genes of circRNAs was examined by the Ualcan. The survival analysis was performed by the Kaplan-Meier plotter.

Results: In comparison with normal lung tissues, LUAD tissues contained 7 overlapping cancer-specific DEcircRNAs with 294 miRNA response elements (MREs). Among the 7 DEcircRNAs, 3 circRNAs (hsa_circ_0072088, hsa_circ_0003528, and hsa_circ_0008274) were upregulated and 4 circRNAs (hsa_circ_0003162, hsa_circ_0029426, hsa_circ_0049271, and hsa_circ_0043256) were downregulated. A circRNA-miRNA-mRNA regulatory network, which included 33 differentially expressed miRNAs (DEmiRNAs) and 2007 differentially expressed mRNAs (DEmRNAs), was constructed. These mRNAs were enriched in the biological function of cell-cell adhesion, response to hypoxia, and stem cell differentiation and were involved in the PI3K-Akt signaling, HIF-1 signaling, and cAMP signaling pathways.

Conclusion: Our results indicated that 7 DEcircRNAs could have diagnostic value for LUAD. Additionally, the circRNAs-mediated ceRNA network might provide a novel perspective into unraveling the pathogenesis and progression of LUAD.
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http://dx.doi.org/10.1155/2021/5193913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448594PMC
September 2021

A -mutant patient with late-stage duodenal adenocarcinoma benefited from anti-HER2 therapy and PD-1 inhibition: a case report.

J Gastrointest Oncol 2021 Aug;12(4):1939-1943

Department of Medical Oncology, Affiliated Hospital of Hebei University, Baoding, China.

Duodenal adenocarcinoma (DA) is a subtype of small bowel adenocarcinoma (SBA). Compared with gastrointestinal cancers such as colorectal cancer and gastric cancer, SBA is less common. For patients with advanced and metastatic DA, chemotherapies are usually extrapolated from colorectal cancer and gastric cancer but the therapeutic effects remain undefined. Herein, we reported a 50-year-old female patient whom was diagnosed as stage IV DA with metastasis to both lungs and retroperitoneal lymph nodes. The next generation sequencing (NGS) using a panel consisting of 168 cancer related genes revealed amplification of the gene which has been a well-recognized therapeutic target among various tumor types. The anti-HER2 targeted therapy trastuzumab was used in combination with XELOX (oxaliplatin and capecitabine) as the first line treatment. The patient achieved partial response (PR) and had progression-free survival (PFS) of six months. After progressive disease (PD), the patient started the second line treatment with trastuzumab and PD1 inhibitors and remained stable disease (SD) with PFS for three months. The use of trastuzumab in neoadjuvant and adjuvant settings have been reported in sporadic cases. To the best of our knowledge, it is the first report to use anti-HER2 therapy and PD-1 inhibition as systemic therapy for advanced DA patients.
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http://dx.doi.org/10.21037/jgo-21-311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421918PMC
August 2021

Alterations of gut microbiota and metabolome with Parkinson's disease.

Microb Pathog 2021 Nov 13;160:105187. Epub 2021 Sep 13.

First Affiliated Hospital of Harbin Medical University, Harbin, 150080, Heilongjiang Province, China. Electronic address:

Gut microbiota and amino acids that are one of their metabolites play important roles in the mechanism of pathology of Parkinson's disease (PD). It has been reported that the level of amino acids in vivo participate in neurodegeneration by regulating adaptive immune response, while the current researches on alteration of amino acids in gut microbiota are still insufficient. We hypothesized that alterations in gut microbiota might be accompanied by altered concentrations of amino acids, leading to the occurrence of PD. In this study, we collected stool samples from PD and healthy controls to analyse fecal microbiome and targeted metabolome by 16S ribosomal RNA (16S rRNA) gene sequencing and gas chromatography coupled to mass spectrometry (GC-MS). At the genus level, there was a greater abundance of Alistipes, Rikenellaceae_RC9_gut_group, Bifidobacterium, Parabacteroides, while Faecalibacterium was decreased in fecal samples from PD patients. Moreover, fecal branched chain amino acids (BCAAs) and aromatic amino acids concentrations were significantly reduced in PD patients compared to controls. Our study not only finds the abundance of certain gut microbiota in PD,but also reveals that it is related to BCAAs and aromatic amino acids. These findings are beneficial to identifying new therapeutic targets for PD by regulating diet and/or gut microbiota.
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http://dx.doi.org/10.1016/j.micpath.2021.105187DOI Listing
November 2021

Corrigendum: miR-124 Alleviates Ischemic Stroke-Induced Neuronal Death by Targeting DAPK1 in Mice.

Front Neurosci 2021 24;15:745087. Epub 2021 Aug 24.

The Brain Cognition and Brain Disease Institute, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

[This corrects the article DOI: 10.3389/fnins.2021.649982.].
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http://dx.doi.org/10.3389/fnins.2021.745087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424333PMC
August 2021

α/Sulfono-γ-AApeptide Hybrid Analogues of Glucagon with Enhanced Stability and Prolonged In Vivo Activity.

J Med Chem 2021 Sep 10;64(18):13893-13901. Epub 2021 Sep 10.

Department of Chemistry, University of South Florida, Tampa, Florida 33620, United States.

Peptide drugs have the advantages of target specificity and good drugability and have become one of the most increasingly important hotspots in new drug research in biomedical sciences. However, peptide drugs generally have low bioavailability and metabolic stability, and therefore, the modification of existing peptide drugs for the purpose of improving stability and retaining activity is of viable importance. It is known that glucagon is an effective therapy for treating severe hypoglycemia, but its short half-life prevents its wide therapeutic use. Herein, we report that combined unnatural residues and long fatty acid conjugation afford potent α/sulfono-γ-AApeptide hybrid analogues of Glucagon with enhanced stability and prolonged in vivo activity. This strategy could be adopted to develop stabilized analogues of other short-acting bioactive peptides.
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http://dx.doi.org/10.1021/acs.jmedchem.1c01289DOI Listing
September 2021

Trigonelline induces autophagy to protect mesangial cells in response to high glucose via activating the miR-5189-5p-AMPK pathway.

Phytomedicine 2021 Nov 29;92:153614. Epub 2021 May 29.

Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University, 1266 Fujin Road, Changchun, Jilin 130021, China. Electronic address:

Background: Diabetic nephropathy (DN) is a primary cause of end-stage renal disease. Increasing evidence indicates that microRNAs (miRNAs) are involved in DN pathogenesis. Trigonelline (TRL) has been shown to lower blood sugar and cholesterol levels, promote nerve regeneration, and exert anti-cancer and sedative properties.

Method: The effect of TRL on human mesangial cell (HMC) growth was assessed using the MTT assay. Differentially expressed miRNAs were validated using real-time quantitative polymerase chain reaction (real-time PCR). Bioinformatics, cell transfection, and Western blot analyses were utilized to confirm the binding of miR-5189-5p to HIF1AN. The effects of miR-5189-5 expression on cell proliferation were also assessed. Western blot analysis was used to determine the activation of multiple signaling molecules including phosphorylated-(p)-AMPK, SIRT1, LC3B, p62, and Beclin-1 in the autophagy pathway.

Results: TRL improved proliferation, increased the expression of miR-5189-5p, reduced HIF1AN, and restored the inhibition of autophagy in HMCs induced by high glucose. MiR-5189-5p mimics inhibited HIF1AN expression, and the miR-5189-5p inhibitor increased HIF1AN expression. MiR-5189-5p mimics significantly improved the proliferation of HMCs induced by high glucose, reduced the relative protein expression of p-AMPK, SIRT1, LC3B, and Beclin-1, and significantly increased the relative protein expression of p62.

Conclusion: We showed that TRL up-regulated miR-5189-5p expression, activated the AMPK pathway, and activated autophagy in HMCs. Our study demonstrates that TRL could be a new treatment strategy to protect mesangial cells in response to high glucose.
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http://dx.doi.org/10.1016/j.phymed.2021.153614DOI Listing
November 2021

Comparative clinicopathological and immunohistochemical study of micronodular thymoma and micronodular thymic carcinoma with lymphoid stroma.

J Clin Pathol 2021 Sep 7. Epub 2021 Sep 7.

Department of Pathology, China Japan Friendship Hospital, Beijing, China

Aim: To elucidate the clinicopathological and immunohistochemical characteristics of micronodular thymomas (MNTs) and micronodular thymic carcinomas (MNCs) with lymphoid stroma.

Methods: We examined four cases of MNTs and three cases of MNCs pathologically and immunohistochemically.

Results: There were prominent cystic changes infive of the seven cases. The neoplasms contained epithelial tumour cells arranged in a micronodular growth pattern lined by cystic walls and separated by abundant lymphoid stroma. Only the tumour cell component of MNCs showed signs of malignancy characterised by cytological atypia and increased mitotic activity. Neoplastic MNC epithelial cells showed strong positivity for CD5 and CD117. However, no immature lymphocytes (TdT-positive and CD99-positive) were present in and around the tumour nodules. None of the patients died or suffered from disease due to MNTs or MNCs.

Conclusion: MNTs and MNCs are rare and less aggressive forms of thymic tumours and can be differentially diagnosed by immunohistochemistry.
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http://dx.doi.org/10.1136/jclinpath-2021-207819DOI Listing
September 2021
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