Publications by authors named "Yan Lv"

265 Publications

A Single Nucleotide Substitution of GSAM Gene Causes Massive Accumulation of Glutamate 1-Semialdehyde and Yellow Leaf Phenotype in Rice.

Rice (N Y) 2021 Jun 5;14(1):50. Epub 2021 Jun 5.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.

Background: Tetrapyrroles play indispensable roles in various biological processes. In higher plants, glutamate 1-semialdehyde 2,1-aminomutase (GSAM) converts glutamate 1-semialdehyde (GSA) to 5-aminolevulinic acid (ALA), which is the rate-limiting step of tetrapyrrole biosynthesis. Up to now, GSAM genes have been successively identified from many species. Besides, it was found that GSAM could form a dimeric protein with itself by x-ray crystallography. However, no mutant of GSAM has been identified in monocotyledonous plants, and no experiment on interaction of GSAM protein with itself has been reported so far.

Result: We isolated a yellow leaf mutant, ys53, in rice (Oryza sativa). The mutant showed decreased photosynthetic pigment contents, suppressed chloroplast development, and reduced photosynthetic capacity. In consequence, its major agronomic traits were significantly affected. Map-based cloning revealed that the candidate gene was LOC_Os08g41990 encoding GSAM protein. In ys53 mutant, a single nucleotide substitution in this gene caused an amino acid change in the encoded protein, so its ALA-synthesis ability was significantly reduced and GSA was massively accumulated. Complementation assays suggested the mutant phenotype of ys53 could be rescued by introducing wild-type OsGSAM gene, confirming that the point mutation in OsGSAM is the cause of the mutant phenotype. OsGSAM is mainly expressed in green tissues, and its encoded protein is localized to chloroplast. qRT-PCR analysis indicated that the mutation of OsGSAM not only affected the expressions of tetrapyrrole biosynthetic genes, but also influenced those of photosynthetic genes in rice. In addition, the yeast two-hybrid experiment showed that OsGSAM protein could interact with itself, which could largely depend on the two specific regions containing the 81th-160th and the 321th-400th amino acid residues at its N- and C-terminals, respectively.

Conclusions: We successfully characterized rice GSAM gene by a yellow leaf mutant and map-based cloning approach. Meanwhile, we verified that OsGSAM protein could interact with itself mainly by means of the two specific regions of amino acid residues at its N- and C-terminals, respectively.
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http://dx.doi.org/10.1186/s12284-021-00492-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179877PMC
June 2021

Probing Permanent Dipole Moments and Removing Exciton Fine Structures in Single Perovskite Nanocrystals by an Electric Field.

Phys Rev Lett 2021 May;126(19):197403

National Laboratory of Solid State Microstructures, School of Physics, and Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.

Single perovskite nanocrystals have emerged as a novel type of semiconductor nanostructure capable of emitting single photons with rich exciton species and fine energy-level structures. Here we focus on single excitons and biexcitons in single perovskite CsPbI_{3} nanocrystals to show, for the first time, how their optical properties are modulated by an external electric field at the cryogenic temperature. The electric field can cause a blueshift in the photoluminescence peak of single excitons, from which the existence of a permanent dipole moment can be deduced. Meanwhile, the fine energy-level structures of single excitons and biexcitons in a single CsPbI_{3} nanocrystal can be simultaneously eliminated, thus preparing a potent platform for the potential generation of polarization-entangled photon pairs.
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http://dx.doi.org/10.1103/PhysRevLett.126.197403DOI Listing
May 2021

A novel peptide ameliorates TNFα- and LPS-induced endothelia dysfunction in preeclampsia.

Am J Hypertens 2021 May 26. Epub 2021 May 26.

Women's Hospital of Nanjing Medical University (Nanjing Maternity and Child Health Care Hospital), Nanjing, 210004, China.

Objective: To investigate the protective effects of the novel peptide AEDPPE on tumor necrosis factor α (TNFα)- and lipopolysaccharide (LPS)-induced injury in the vascular endothelium in preeclampsia.

Methods: The effects of AEDPPE on TNFα-induced vascular endothelial injury were assessed by enzyme-linked immunosorbent assay (ELISA), quantitative real-time quantitative PCR (qRT-PCR), mitochondrial membrane potential assay, cell counting kit-8(CCK-8) assay, THP-1 monocyte-human umbilical vein endothelial cell(HUVEC) adhesion assay, endothelial tube-forming assay, transcriptomic analysis, preeclamptic symptom analysis and histological analysis in preeclampsia-like rat models induced by LPS.

Results: AEDPPE alleviated the upregulation of antiangiogenic factors including sFlt-1, ET-1 and tPA and attenuated the reduction in mitochondrial potential induced by TNFα in HUVECs.In addition, AEDPPE treatment counteracted the decrease in tube formation and decreased the numbers of THP-1 monocytes attached to HUVECs caused by TNFα. Mechanistically, cytokine -cytokine receptor interactions enriched many genes and the TNF signaling pathway may be involved in this phenomenon. Moreover, cotreatment with LPS and AEDPPE significantly reversed the preeclampsia-like phenotype including hypertension and proteinuria and improved the functions of the kidney and placenta.

Conclusions: AEDPPE effectively ameliorated the vascular endothelial injury induced by TNFα and LPS in preeclampsia. We suggest that AEDPPE may be a novel therapeutic candidate for preeclampsia treatment. These findings demonstrate that AEDPPE may play an effective role in ameliorating vascular endothelial dysfunction and be a potential therapeutic agent for preeclampsia.
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http://dx.doi.org/10.1093/ajh/hpab086DOI Listing
May 2021

Acute flaccid paralysis and neurogenic respiratory failure associated with enterovirus D68 infection in children: Report of two cases.

World J Clin Cases 2021 May;9(14):3327-3333

Department of Hyperbaric Oxygen, The Sixth Medical Center of PLA General Hospital, Beijing 100048, China.

Background: Acute flaccid paralysis (AFP) and neurogenic respiratory failure rarely occur in children. At the end of 2018, some children with such symptoms were admitted to our hospital. In this study, we aimed to assess two children with AFP and neurogenic respiratory failure associated with enterovirus D68 (EV-D68).

Case Summary: Two children admitted to our hospital presented with symptoms and imaging results different from those of acute disseminated encephalomyelitis and hand, foot, and mouth disease. Their main symptoms were AFP and neurogenic respiratory failure. Magnetic resonance imaging showed severe inflammatory injury mainly to the anterior horn cells of the spinal cord. Blood and cerebrospinal fluid samples were collected to assess for pathogens, including bacteria, tuberculosis, cryptococcus, herpes virus, and coxsackie virus, and the results were negative. At the beginning, the two cases were not assessed for EV-D68 in the nasopharyngeal, blood, and cerebrospinal fluid specimens. About 2 mo later, EV-D68 was detected in the stool sample of one of the cases. The symptom of AFP was caused by injury to the anterior horn cells at levels C5-L5 of the spinal cord, while neurogenic respiratory failure was at levels C3-C5.

Conclusion: We should pay attention to the detection and diagnosis of EV-D68 and make efforts to develop antivirus drugs and vaccines.
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http://dx.doi.org/10.12998/wjcc.v9.i14.3327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107912PMC
May 2021

Minocycline alleviates Gulf War Illness rats via altering gut microbiome, attenuating neuroinflammation and enhancing hippocampal neurogenesis.

Behav Brain Res 2021 Jul 14;410:113366. Epub 2021 May 14.

Department of Neurology, The General Hospital of Northern Theater Command, Shenyang, Liaoning, China. Electronic address:

Accumulating evidences suggest that deficits in neurogenesis, chronic inflammation and gut microbiome dysregulation contribute to the pathophysiology of Gulf War Illness (GWI). Minocycline has been demonstrated to be a potent neuroprotective agent and could regulate neuroinflammation. The present study intends to investigate whether the treatment of minocycline maintains better cognition and mood function in a rat model of GWI and the potential mechanism. Rats received 28 days of GWI-related chemical exposure and restraint stress, along with daily minocycline or vehicle treatment. Cognitive and mood function, neuroinflammation, neurogenesis and gut microbiota were detected. We found that minocycline treatment induces better cognitive and mood function in the GWI rat model, as indicated by open-field test, elevated plus maze test, novel object recognition test and forced swim test. Moreover, minocycline treatment reversed the altered gut microbiome, neuroinflammation and the decreased hippocampal neurogenesis of rats with GWI. Taken together, our study indicated that minocycline treatment exerts better cognitive and mood function in GWI rat model, which is possibly related to gut microbiota remodeling, restrained inflammation and enhanced hippocampal neurogenesis. These results may establish minocycline as a potential prophylactic or therapeutic agent for the treatment of GWI.
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http://dx.doi.org/10.1016/j.bbr.2021.113366DOI Listing
July 2021

LncRNA PINK1-AS promotes Gαi1-driven gastric cancer tumorigenesis by sponging microRNA-200a.

Oncogene 2021 Jun 6;40(22):3826-3844. Epub 2021 May 6.

Center of Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University, Suzhou, China.

Gastric cancer (GC) is one of the leading causes of human mortality around the world. We have previously shown that Gαi1 (the inhibitory subunit 1 of the heterotrimeric guanine nucleotide-binding protein) recruitment to ligand-activated receptor tyrosine kinases (RTKs) is essential for signaling. Testing its role in GC cancer-promoting functions, we found that Gαi1 is upregulated in human GC, correlating with poor overall survival. In established and primary human GC cells, Gαi1 shRNA (small hairpin RNA) or knockout produced significant anti-GC cell activity, proliferation and migration was inhibited, and apoptosis was activated. Conversely, ectopic Gαi1 overexpression promoted proliferation and migration of GC cells in vitro. By examining the tumor-suppressive miRNA microRNA-200a (miR-200a), we found that miR-200a directly silenced Gαi1 to induce anti-GC cell activity. The expression of miR-200a was downregulated in human GC, correlating with upregulation of a novel miR-200a-targeting long non-coding RNA (LncRNA), PINK1 (PTEN Induced Kinase 1)-AS. RNA immunoprecipitation, RNA-pull down, and RNA fluorescence in situ hybridization assays confirmed that PINK1-AS directly binds to miR-200a. Silencing PINK1-AS in GC cells led to miR-200a accumulation, Gαi1 downregulation, and inhibition of GC cell progression in vitro, whereas PINK1-AS upregulation produced the converse results. Significantly, anti-GC cell activity induced by PINK1-AS shRNA was ameliorated by the expression of miR-200a antisense or the 3'-UTR (untranslated region)-depleted Gαi1. In vivo, the growth of subcutaneous MGC-803 xenografts in nude mice was inhibited by PINK1-AS shRNA, but accelerated by PINK1-AS overexpression. Patient-derived GC xenograft growth in nude mice was largely inhibited after intratumoral injection of PINK1-AS shRNA lentivirus. In conclusion, PINK1-AS promotes Gαi1-driven GC progression by sponging miR-200a.
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http://dx.doi.org/10.1038/s41388-021-01812-7DOI Listing
June 2021

Comparison between voltammetric detection methods for abalone-flavoring liquid.

Open Life Sci 2021 15;16(1):354-361. Epub 2021 Apr 15.

Mechanical Engineering Department of Dalian Polytechnic University, Dalian 116034, China.

This article attempts to determine the most accurate classification method for different abalone-flavoring liquids. Three common voltammetric detection methods, namely, linear sweep voltammetry (LSV), cyclic voltammetry (CV), and square-wave voltammetry (SWV), were considered. To compare their classification accuracies of abalone-flavoring liquids, three methods were separately adopted to classify five different abalone-flavoring liquids, using a four-electrode (Au, Pt, Pd, and W) sensor array. Then the data acquired by each method were subject to the principal component analysis (PCA): the first three principal components whose eigenvalues were greater than 1 were extracted from each set of data; the cumulative variance contribution rate and the principal component scores of each method were obtained. The PCA results show that the first three principal components obtained by the CV had the highest cumulative variance contribution rate (91.307%), indicating that the CV can more comprehensively characterize the information of abalone-flavoring liquid samples than the other two methods. According to the principal component scores, compared with those of LSV and SWV, the same kind of samples detected by the CV were highly clustered and the different kinds of samples detected by the CV were greatly dispersed. This indicates that the CV can effectively distinguish between the five abalone-flavoring liquids. Finally, the detection data were further verified through probabilistic neural network and a support vector machine algorithm optimized by genetic algorithm. The results further confirm that the CV is more accurate than the other two methods in the classification of abalone-flavoring liquids. Therefore, the CV was recommended for the classification of abalone-flavoring liquids.
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http://dx.doi.org/10.1515/biol-2021-0035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051168PMC
April 2021

Protective and Risk Factors for Medical and Nursing Staff Suffering From Psychological Symptoms During COVID-19.

Front Psychol 2021 16;12:603553. Epub 2021 Apr 16.

Department of Neurology, Hainan General Hospital, Haikou, China.

With the outbreak of the coronavirus disease 2019 (COVID-19) epidemic in China, the general public but also medical staff were confronted with psychological challenges, suffering from the highly infectious and unknown characteristics of COVID-19. In this study, we surveyed psychological symptoms including anxiety, depression, and sleep disorders in medical staff. A questionnaire star/WeChat link-based survey assessing the Generalized Anxiety Disorder 7-item scale, Patient Health Questionnaire-9 depression, the Insomnia Severity Index, Social Support scales in addition to lifestyle, and income level was conducted and included 8,288 medical staff from 24 provinces in China. Pearson Chi-square and Mann-Whitney -tests were used to evaluate single risk factors and significant differences in psychological symptoms before and during the outbreak of COVID-19. Binary logistic regression analyses were conducted for the risk factors of anxiety, depression, and sleep disorder symptoms. Medical staff had a high incidence of psychological symptoms, which was more prominent during the COVID-19 epidemic. Comparatively, females, nurses, first-line department, never exercised, and low income were risk factors for psychological symptoms. Social support including objective support, subjective support, support utility, and regular sports over 3 times per week were protective and manageable elements that could protect from and manage the psychological symptoms of medical staff. The susceptibility of psychological symptoms among medical staff should be of concern to policymakers and the public in the long-term, and the aggravation of mental health problems of medical staff could be eased by providing adequate social support during and after the COVID-19 outbreak.
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http://dx.doi.org/10.3389/fpsyg.2021.603553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086510PMC
April 2021

Catalase (CAT) Gene Family in Rapeseed ( L.): Genome-Wide Analysis, Identification, and Expression Pattern in Response to Multiple Hormones and Abiotic Stress Conditions.

Int J Mol Sci 2021 Apr 20;22(8). Epub 2021 Apr 20.

College of Agriculture, Engineering Research Center of Ecology and Agricultural Use of Wetland of Ministry of Education, Yangtze University, Jingzhou 434025, China.

Catalase (CAT) is an antioxidant enzyme expressed by the gene family and exists in almost all aerobic organisms. Environmental stresses induce the generation of reactive oxygen species (ROS) that eventually hinder plant growth and development. The CAT enzyme translates the hydrogen peroxide (HO) to water (HO) and reduce the ROS levels to shelter the cells' death. So far, the gene family has not been reported in rapeseed ( L.). Therefore, a genome-wide comprehensive analysis was conducted to classify the genes in the rapeseed genome. The current study identified 14 genes in the rapeseed genome. Based on phylogenetic and synteny analysis, the belong to four groups (Groups I-IV). A gene structure and conserved motif analysis showed that Group I, Group II, and Group IV possess almost the same intron/exon pattern, and an equal number of motifs, while Group III contains diverse structures and contain 15 motifs. By analyzing the -elements in the promoters, we identified five hormone-correlated responsive elements and four stress-related responsive elements. Further, six putative bna-miRNAs were also identified, targeting three genes ( and ). Gene ontology (GO) enrichment analysis showed that the genes were largely related to cellular organelles, ROS response, stimulus response, stress response, and antioxidant enzymes. Almost 10 genes showed higher expression levels in different tissues, i.e., root, leaf, stem, and silique. The expression analysis showed that and were significantly upregulated by cold, salinity, abscisic acid (ABA), and gibberellic acid (GA) treatment, but not by drought and methyl jasmonate (MeJA). Notably, most of the genes were upregulated by waterlogging stress, except , and . Our results opened new windows for future investigations and provided insights into the family genes in rapeseed.
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http://dx.doi.org/10.3390/ijms22084281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074368PMC
April 2021

Expert consensus of the Chinese Association for the Study of Pain on pain treatment with the transdermal patch.

World J Clin Cases 2021 Mar;9(9):2110-2122

Department of Algology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.

Chronic pain lasting more than 3 mo, or even several years can lead to disability. Treating chronic pain safely and effectively is a critical challenge faced by clinicians. Because administration of analgesics through oral, intravenous or intramuscular routes is not satisfactory, research toward percutaneous delivery has gained interest. The transdermal patch is one such percutaneous delivery system that can deliver drugs through the skin and capillaries at a certain rate to achieve a systemic or local therapeutic effect in the affected area. It has many advantages including ease of administration and hepatic first pass metabolism avoidance as well as controlling drug delivery, which reduces the dose frequency and side effects. If not required, then the patch can be removed from the skin immediately. The scopolamine patch was the first transdermal patch to be approved for the treatment of motion sickness by the Food and Drug Administration in 1979. From then on, the transdermal patch has been widely used to treat many diseases. To date, no guidelines or consensus are available on the use of analgesic drugs through transdermal delivery. The pain branch of the Chinese Medical Association, after meeting and discussing with experts and based on clinical evidence, developed a consensus for promoting and regulating standard use of transdermal patches containing analgesic drugs.
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http://dx.doi.org/10.12998/wjcc.v9.i9.2110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017498PMC
March 2021

Chinese Association for the Study of Pain: Expert consensus on chronic postsurgical pain.

World J Clin Cases 2021 Mar;9(9):2090-2099

Department of Algology, The Affiliated T.C.M Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.

Chronic postsurgical pain is a common surgical complication that severely reduces a patient's quality of life. Many perioperative interventions and management strategies have been developed for reducing and managing chronic postsurgical pain. Under the leadership of the Chinese Association for the Study of Pain, an editorial committee was formed for chronic postsurgical pain diagnosis and treatment by experts in relevant fields. The editorial committee composed the main content and framework of this consensus and established a working group. The working group conducted literature review (1989-2020) using key words such as "surgery", "post-surgical", "post-operative", "pain", "chronic", and "persistent" in different databases including MEDLINE, EMBASE, PubMed, Cochrane Central Register of Controlled Trials and Cochrane Database of Systematic Reviews. Only publications in the English language were included. The types of literature included systematic reviews, randomized controlled studies, cohort studies and case reports. This consensus was written based on clinical practice combined with literature evidence. The first draft of the consensus was rigorously reviewed and edited by all the editorial committee experts before being finalized. The level of evidence was assessed by methodological experts based on the Oxford Centre for Evidence-Based Medicine Levels of Evidence. The strength of recommendation was evaluated by all editorial committee experts, and the opinions of most experts were adopted as the final decision. The recommendation level "strong" generally refers to recommendations based on high-level evidence and consistency between clinical behavior and expected results. The recommendation level "weak" generally refers to the uncertainty between clinical behavior and expected results based on low-level evidence.
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http://dx.doi.org/10.12998/wjcc.v9.i9.2090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017506PMC
March 2021

Expert consensus on the diagnosis and treatment of myofascial pain syndrome.

World J Clin Cases 2021 Mar;9(9):2077-2089

Department of Algology, The Third Xiangya Hospital of Central South University, Changsha 410001, Hunan Province, China.

Myofascial pain syndrome (MPS) is characterized by myofascial trigger points and fascial constrictions. At present, domestic and foreign scholars have not reached a consensus on the etiology and pathogenesis of MPS. Due to the lack of specific laboratory indicators and imaging evidence, there is no unified diagnostic criteria for MPS, making it easy to confuse with other diseases. The Chinese Association for the Study of Pain organized domestic experts to formulate this Chinese Pain Specialist Consensus on the diagnosis and treatment of MPS. This article reviews relevant domestic and foreign literature on the definition, epidemiology, pathogenesis, clinical manifestation, diagnostic criteria and treatments of MPS. The consensus is intended to normalize the diagnosis and treatment of MPS and be used by first-line doctors, including pain physicians to manage patients with MPS.
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http://dx.doi.org/10.12998/wjcc.v9.i9.2077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017503PMC
March 2021

Expert panel's guideline on cervicogenic headache: The Chinese Association for the Study of Pain recommendation.

World J Clin Cases 2021 Mar;9(9):2027-2036

Department of Algology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

Cervicogenic headache (CEH) has been recognized as a unique category of headache that can be difficult to diagnose and treat. In China, CEH patients are managed by many different specialties, and the treatment plans remain controversial. Therefore, there is a great need for comprehensive evidence-based Chinese experts' recommendations for the management of CEH. The Chinese Association for the Study of Pain asked an expert panel to develop recommendations for a series of questions that are essential for daily clinical management of patients with CEH. A group of multidisciplinary Chinese Association for the Study of Pain experts identified the clinically relevant topics in CEH. A systematic review of the literature was performed, and evidence supporting the benefits and harms for the management of CEH was summarized. Twenty-four recommendations were finally developed through expert consensus voting for evidence quality and recommendation strength. We hope this guideline provides direction for clinicians and patients making treatment decisions for the management of CEH.
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http://dx.doi.org/10.12998/wjcc.v9.i9.2027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017501PMC
March 2021

Exciton-acoustic phonon coupling revealed by resonant excitation of single perovskite nanocrystals.

Nat Commun 2021 Apr 13;12(1):2192. Epub 2021 Apr 13.

National Laboratory of Solid State Microstructures, School of Physics, and Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, 210093, China.

Single perovskite nanocrystals have attracted great research attention very recently due to their potential quantum-information applications, which critically depend on the development of powerful optical techniques to resolve delicate exciton photophysics. Here we have realized resonant and near-resonant excitations of single perovskite CsPbI nanocrystals, with the scattered laser light contributing to only ~10% of the total collected signals. This allows us to estimate an ultranarrow photoluminescence excitation linewidth of ~11.32 µeV for the emission state of a single CsPbI nanocrystal, corresponding to an exciton dephasing time of ~116.29 ps. Meanwhile, size-quantized acoustic phonons can be resolved from a single CsPbI nanocrystal, whose coupling with the exciton is proposed to arise from the piezoelectric potential. The ability to collect resonance fluorescence from single CsPbI nanocrystals, with the subsequent revelation of exciton-acoustic phonon coupling, has marked a critical step towards their steady advancement into superior quantum-light sources.
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http://dx.doi.org/10.1038/s41467-021-22486-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044187PMC
April 2021

Melatonin inhibits seed germination by crosstalk with abscisic acid, gibberellin, and auxin in Arabidopsis.

J Pineal Res 2021 May 20;70(4):e12736. Epub 2021 Apr 20.

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China.

Seed germination, an important developmental stage in the life cycle of seed plants, is regulated by complex signals. Melatonin is a signaling molecule associated with seed germination under stressful conditions, although the underlying regulatory mechanisms are largely unknown. In this study, we showed that a low concentration (10 µM or 100 µM) of melatonin had no effect on seed germination, but when the concentration of melatonin increased to 500 µM or 1000 µM, seed germination was significantly inhibited in Arabidopsis. RNA sequencing analysis showed that melatonin regulated seed germination correlated to phytohormones abscisic acid (ABA), gibberellin (GA), and auxin. Further investigation revealed that ABA and melatonin synergistically inhibited seed germination, while GA and auxin antagonized the inhibitory effect of seed germination by melatonin. Disruption of the melatonin biosynthesis enzyme gene serotonin N-acetyltransferase (SNAT) or N-acetylserotonin methyltransferase (ASMT) promoted seed germination, while overexpression of ASMT inhibited seed germination. Taken together, our study sheds new light on the function and mechanism of melatonin in modulating seed germination in Arabidopsis.
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http://dx.doi.org/10.1111/jpi.12736DOI Listing
May 2021

Infestation and seasonal fluctuation of chigger mites on the Southeast Asian house rat () in southern Yunnan Province, China.

Int J Parasitol Parasites Wildl 2021 Apr 11;14:141-149. Epub 2021 Feb 11.

Vector Laboratory, Institute of Pathogens and Vectors, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali University, Dali, Yunnan Province, 671000, China.

Chigger mites are the common ectoparasites of rodents and the exclusive vector of scrub typhus. The Southeast Asian house rat () is an important reservoir host and infectious source of some zoonoses including scrub typhus. From April 2016 to March 2017, a 12-month consecutive investigation was made at Jingha village in southern Yunnan of China, which is an important focus of scrub typhus. The infestation and seasonal fluctuation of chigger mites on were studied based on the investigation. From 2,053 captured , a total of 99,221 chiggers were collected and identified as comprising 102 species with very high species diversity. The richness (), diversity index (), evenness () and dominance index () of the chigger community on the rat varied in different months. Of the 102 chigger species, five main species accounted for 84.81% of the total chiggers (84,147/99,221). The five main chiggers were (.) (32.65%), (24.68%), (.) (19.02%), (.) (4.63%) and (.) (3.83%). Of the five chigger species, (.) and (.) are the most important vectors of scrub typhus in China. The five chigger species showed different patterns of seasonal fluctuation. The seasonal fluctuation of (.) belonged to summer-autumn type with the highest peak in July, but (.) mainly appeared in winter and spring with the peak from January to February. The temperature and rainfall were two key factors which influenced the seasonal fluctuation of chigger mites.
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http://dx.doi.org/10.1016/j.ijppaw.2021.02.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898075PMC
April 2021

The social stigma of infertile women in Zhejiang Province, China: a questionnaire-based study.

BMC Womens Health 2021 03 5;21(1):97. Epub 2021 Mar 5.

Ambulatory Surgery Center, Women's Hospital, Zhejiang University School of Medicine, No. 1 Xueshi Road, Hangzhou, Zhejiang Province, China.

Background: Infertile women often face stigmatization worldwide. This study aimed to investigate the stigma against infertile women in China and to analyze its influencing factors.

Methods: Of 270 women who were randomly selected from patients receiving adjuvant fertility treatment in Zhejiang Province, China, 254 successfully completed the general information questionnaire, disease information questionnaire, and Chinese version of the infertility stigma scale (ISS). The ISS contained 27 positively worded items, each of which was graded on a 5-point Likert-type scale.

Results: The total stigma score of female infertility patients was 66.39 ± 21.96. By dividing the number of items, the average score for each ISS item was 2.13 ± 0.81, indicating the presence of stigma. Among the four ISS factors, the social withdrawal score was the highest (2.64 ± 1.05), whereas the family stigma score was the lowest (1.88 ± 0.88). Multiple stepwise regression analysis further revealed that the duration of infertility and monthly income were important predictors of the stigma of infertile women.

Conclusions: Infertile women experience moderate to high levels of stigma in Zhejiang, China. Thus, supportive psychological interventions and public education are required to change patients' cognition and assist patients in coping with negative experiences.
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http://dx.doi.org/10.1186/s12905-021-01246-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934237PMC
March 2021

A Novel Carbon Support: Few-Layered Graphdiyne-Decorated Carbon Nanotubes Capture Metal Clusters as Effective Metal-Supported Catalysts.

Small 2021 Mar 3;17(12):e2006442. Epub 2021 Mar 3.

Key Laboratory of Energy Materials Chemistry, Ministry of Education; Key Laboratory of Advanced Functional Materials, Autonomous Region, Institute of Applied Chemistry, College of Chemistry, Xinjiang University, Urumqi, Xinjiang, 830046, P.R. China.

Carbon-supported metal nanocatalysts have received substantial attention for heterogeneous catalysis in industry. Hunting for suitable and impactful carbon supports that have strong interactions with metal nanocatalyst is a matter of great urgency. Herein, a well-designed graphdiyne layer decorated on the carbon nanotubes sidewalls ([email protected]) serves as a novel carbon support. This unique hybrid structure effectively traps platinum and palladium atomic clusters (Pt/Pd-ACs) with dimensions of 0.65 nm and 1.05 nm uniformly and firmly, forming novel carbon-supported metal nanocatalysts (Pt(Pd)-ACs/CNT @ GDY) for efficient hydrogen generation and aromatic nitroreduction, respectively. The Pt-ACs/[email protected] can deliver an HER current density of 10 mA cm with a small overpotential of 23 mV in 0.5 M H SO , showing a greatly enhanced mass activity, intrinsic activity than the commercial Pt/C catalyst. The Pd-ACs/[email protected] also exhibits excellent catalytic activity and a high turnover frequency of 38.0 min for aromatic nitroreduction. The carbon support turns out to possess excellent conductivity, abundant and uniform reactive sites, low redox potential, more negative surface and large specific surface area as well as a strong interaction with ACs, as anticipated in ideal supports, which can be applied in other metal-supported catalysts.
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http://dx.doi.org/10.1002/smll.202006442DOI Listing
March 2021

Effect of morphology and phase engineering of MoS on electrochemical properties of carbon nanotube/[email protected] composites.

J Colloid Interface Sci 2021 May 27;590:591-600. Epub 2021 Jan 27.

Fujian Provincial Key Laboratory of Fire Retardant Materials, College of Materials, Xiamen University, Xiamen, 361005, China.

This paper rationally designs the morphology and phase structure of carbon nanotube/[email protected] (CNT/[email protected]) composites, with MoS conductive wrapping growing vertically on the outer layer of the composites via hydrothermal method. The crystalline nature and chemical properties are characterized by X-ray diffraction (XRD), Flourier transformation infrared spectroscopy (FT-IR), Raman spectroscopy (Raman), X-ray photoelectron spectroscopy (XPS). Morphology and microstructures are determined by Scanning electric microscopy (SEM), Transmission electron microscope (TEM) and Brunauer-Emmett-Teller (BET). The developed composites possess excellent electrochemical properties (the specific capacitance is substantially increased by ~119%, reaching 700.0 F g after wrapping by MoS) and good cycling stability (after over 5000 cycles retains 80.8% capacitance) in three-electrode systems, which indicating that the unique morphology of MoS shells endow the channels to composites for rapid charge transport and ionic diffusion. Furthermore, symmetric supercapacitors devices assembled with the CNT/[email protected] composites achieve specific capacitance of 459.7 F g at 1 A g, capacitance retention is 97.4% after 10,000 cycles and reach superior energy density of 40.9 Wh kg at the power density of 400 W kg. This strategy of three-dimensional wrapping method may open up a new potential to relieve the dilemma of degraded performance of supercapacitor, while improving the capacitance and stability for supercapacitors.
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http://dx.doi.org/10.1016/j.jcis.2021.01.051DOI Listing
May 2021

Cytogenetic characteristics of 665 patients with myelodysplastic syndrome in China: A single-center report.

Oncol Lett 2021 Feb 17;21(2):126. Epub 2020 Dec 17.

Department of Hematology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, P.R. China.

The karyotype is highly important for diagnosis and prognosis in myelodysplastic syndrome (MDS). The objective of the present study was to investigate the cytogenetic characteristics of patients with MDS in China. The karyotypes of 665 Chinese patients with MDS were analyzed, and it was identified that 298 cases (298/665, 44.8%) had abnormal karyotypes. Among the 298 patients with abnormal karyotypes, the 75 patients with trisomy 8 (+8) constituted the most common subset (75/298, 25.2%). The incidence of abnormal karyotypes was significantly higher in patients who were ≥51 years old compared with those <51 years old, (54.8 vs. 34.7%, respectively; P<0.05). Based on World Health Organization (WHO) classification-based Prognostic Scoring System (WPSS) criteria, the incidence of poor-prognosis karyotypes was significantly higher (17.4 vs. 5.4%; P<0.05) in the older patient group, and based on the Revised International Prognostic Scoring System (IPSS-R) criteria, the incidence of poor-/very poor-prognosis karyotypes was also significantly higher (17.4 vs. 6.6%; P<0.05) in patients ≥51 years old compared with younger ones. Based on the WHO classification of MDS subtypes, the incidence of abnormal karyotypes in patients with high percentages of bone marrow (BM) blasts [excess blasts (EB)-I + EB-II, ≥5% blasts] was significantly higher than that in patients with low percentages of BM blasts (those with single lineage dysplasia + multilineage dysplasia, <5% blasts) (62.5 vs. 36.0%; P<0.05). The incidence of poor-prognosis karyotypes based on WPSS criteria was significantly higher in patients with high percentages of BM blasts than those with low percentages (22.0 vs. 6.9%, respectively; P<0.05), and the incidence of poor-/very poor-prognosis karyotypes based on IPSS-R criteria was also significantly higher (23.0 vs. 7.4%, respectively; P<0.05). These results demonstrate that +8 is the most common abnormal karyotype in Chinese patients with MDS. Age and the percentage of BM blasts are associated with the incidence of both abnormal karyotypes and karyotypes with poor prognosis. The results of cytogenetic abnormalities in this study will supplement the data on patients of MDS in China.
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http://dx.doi.org/10.3892/ol.2020.12387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798047PMC
February 2021

Melatonin confers heavy metal-induced tolerance by alleviating oxidative stress and reducing the heavy metal accumulation in Exophiala pisciphila, a dark septate endophyte (DSE).

BMC Microbiol 2021 02 5;21(1):40. Epub 2021 Feb 5.

State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Yunnan University, Kunming, China.

Background: Melatonin (MT), ubiquitous in almost all organisms, functions as a free radical scavenger. Despite several reports on its role as an antioxidant in animals, plants, and some microorganisms, extensive studies in filamentous fungi are limited. Based upon the role of melatonin as an antioxidant, we investigated its role in heavy metal-induced stress tolerance in Exophiala pisciphila, a dark septate endophyte (DSE), by studying the underlying mechanisms in alleviating oxidative stress and reducing heavy metal accumulation.

Results: A significant decrease in malondialdehyde (MDA) and oxygen free radical (OFR) in E. pisciphila was recorded under Cd, Zn, and Pb stresses as compared to the control. Pretreatment of E. pisciphila with 200.0 μM exogenous melatonin significantly increased the activity of superoxide dismutase (SOD) under Zn and Pb stresses. Pretreatment with 200.0 μM melatonin also lowered Cd, Zn, and Pb concentrations significantly. Melatonin production was enhanced by Cd, Cu, and Zn after 2 d, and melatonin biosynthetic enzyme genes, E. pisciphila tryptophan decarboxylase (EpTDC1) and serotonin N-acetyltransferase (EpSNAT1), were transcriptionally upregulated. The overexpression of EpTDC1 and N-acetylserotonin O-methyltransferase (EpASMT1) in Escherichia coli and Arabidopsis thaliana enhanced its heavy metal-induced stress tolerance. The overexpression of EpTDC1 and EpASMT1 reduced the Cd accumulation in the whole A. thaliana plants, especially in the roots.

Conclusions: Melatonin conferred heavy metal-induced stress tolerance by alleviating oxidative stress, activating antioxidant enzyme SOD, and reducing heavy metal accumulation in E. pisciphila. Melatonin biosynthetic enzyme genes of E. pisciphila also played key roles in limiting excessive heavy metal accumulation in A. thaliana. These findings can be extended to understand the role of melatonin in other DSEs associated with economically important plants and help develop new strategies in sustainable agriculture practice where plants can grow in soils contaminated with heavy metals.
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http://dx.doi.org/10.1186/s12866-021-02098-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7863494PMC
February 2021

Camrelizumab combined with lenvatinib in the treatment of gastric cancer with liver metastasis: a case report.

Ann Palliat Med 2021 Jan;10(1):803-809

Department of Integrated Traditional Chinese Medicine and Western Medicine, West China Hospital of Sichuan University, Chengdu, China. Email:

We report a rare case of gastric cancer with liver metastasis in a patient whose condition improved with the combination use of lenvatinib and camrelizumab. A 53-year-old man was admitted to hospital for abdominal pain. After an enhanced CT scan of his abdomen, it was found that he had multiple liver occupancy. Gastroscopic biopsies and liver biopsies supported the clinical diagnosis of stage IV gastric cancer. Next-generation sequencing technology (NGS) testing indicated that the tumor mutational burden (TMB) of the liver tissue and stomach tissue samples were high. The patient first received transcatheter arterial chemoembolization (TACE) treatment on August 30, 2018. The patient later received combined lenvatinib (8 mg, daily) anti-angiogenic therapy and camrelizumab (200 mg, every 2 weeks) immunotherapy. Three months following treatment, the patient's abdominal pain was relieved and his weight was increased. Regular reexamination of CT showed that the metastases of the liver continued to shrink. The curative effect was evaluated as partial response (PR). After 11 months of treatment, gastroscopy showed that the lesions of gastric tumors disappeared. At present, 14 months since the initial treatment, there is no clinical evidence of disease progression, and the current overall survival time is 14 months. Moreover, the quality of life of the patient was good and there were no obvious adverse drug reactions. This suggests that immunotherapy combined with antiangiogenic therapy has a synergistic effect on tumor and can play a better anti-tumor effect.
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http://dx.doi.org/10.21037/apm-20-2572DOI Listing
January 2021

Understanding Patterns of Brain Metastasis in Triple-Negative Breast Cancer and Exploring Potential Therapeutic Targets.

Onco Targets Ther 2021 22;14:589-607. Epub 2021 Jan 22.

The Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing 210009, People's Republic of China.

Triple-negative breast cancer (TNBC) is a highly malignant subtype of breast cancer. High invasiveness and heterogeneity, as well as a lack of drug targets, are the main factors leading to poor prognosis. Brain metastasis (BM) is a serious event threatening the life of breast cancer patients, especially those with TNBC. Compared with that for hormone receptor-positive and HER2-positive breast cancers, TNBC-derived BM (TNBCBM) occurs earlier and more frequently, and has a worse prognosis. There is no standard treatment for BM to date, and one is urgently required. In this review, we discuss the current knowledge regarding the developmental patterns of TNBCBM, focusing on the key events in BM formation. Specifically, we consider (i) the nature and function of TNBC cells; (ii) how TNBC cells cross the blood-brain barrier and form a fenestrated, more permeable blood-tumor barrier; (iii) the biological characteristics of TNBCBM; and (iv) the infiltration and colonization of the central nervous system (CNS) by TNBC cells, including the establishment of premetastatic niches, immunosurveillance escape, and metabolic adaptations. We also discuss putative therapeutic targets and precision therapy with the greatest potential to treat TNBCBM, and summarize the relevant completed and ongoing clinical trials. These findings may provide new insights into the prevention and treatment of BM in TNBC patients.
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http://dx.doi.org/10.2147/OTT.S293685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837592PMC
January 2021

Immobilization persistence of Cu, Cr, Pb, Zn ions by the addition of steel slag in acidic contaminated mine soil.

J Hazard Mater 2021 Jun 19;412:125176. Epub 2021 Jan 19.

Department of Metallurgy, Graduate School of Engineering, Tohoku University, 02 Aoba-yama, Sendai 980-8579, Japan.

Adding steel slag to the acidic contaminated mine soil can immobilize heavy metal ions, but immobilization persistence of the metal ions needs to be determined. In this study, dynamic column simulation experiments were set up to compare the immobilization persistence of Cu, Cr, Pb and Zn ions in original soil and with the addition of slag, lime or fly ash to the soil during a simulated 36-month of acid rain leaching. After adding slag and lime, the pH, organic matter content and cation exchange capacity of soil were significantly increased. Compared with the original soil, additions of slag and lime to the soil were able to persistently immobilize the metal ions, whereas fly ash additions had little effect. During simulation, the metal ion concentrations in the slag group leaching solution were essentially consistent with Standard IV for groundwater. The metal ions were immobilized to form instable hydroxides and stable fractions following adding slag to soil. The hydroxide could rerelease metal ions by acid rain leaching, part of which were re-immobilized into stable fractions by entering slag lattice and complexing with soil organic matter. Therefore, adding slag to soil can persistently immobilize metal ions for heavy metal-contaminated acidic mine soil.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125176DOI Listing
June 2021

Genome Sequence of " Dehalogenimonas etheniformans" Strain GP, a Vinyl Chloride-Respiring Anaerobe.

Microbiol Resour Announc 2020 Dec 10;9(50). Epub 2020 Dec 10.

Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA

" Dehalogenimonas etheniformans" strain GP couples growth with the reductive dechlorination of vinyl chloride and several polychlorinated ethenes. The genome sequence comprises a circular 2.07-Mb chromosome with a G+C content of 51.9% and harbors 50 putative reductive dehalogenase genes.
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http://dx.doi.org/10.1128/MRA.01212-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729418PMC
December 2020

Prevalence, risk factors, and management of dementia and mild cognitive impairment in adults aged 60 years or older in China: a cross-sectional study.

Lancet Public Health 2020 12;5(12):e661-e671

Department of Psychiatry, Zhejiang Provincial People's Hospital, Zhejiang, China.

Background: China has a large population of older people, but has not yet undertaken a comprehensive study on the prevalence, risk factors, and management of both dementia and mild cognitive impairment (MCI).

Methods: For this national cross-sectional study, 46 011 adults aged 60 years or older were recruited between March 10, 2015, and Dec 26, 2018, using a multistage, stratified, cluster-sampling method, which considered geographical region, degree of urbanisation, economic development status, and sex and age distribution. 96 sites were randomly selected in 12 provinces and municipalities representative of all socioeconomic and geographical regions in China. Participants were interviewed to obtain data on sociodemographic characteristics, lifestyle, medical history, current medications, and family history, and then completed a neuropsychological testing battery administered by a psychological evaluator. The prevalence of dementia (Alzheimer's disease, vascular dementia, and other dementias) and MCI were calculated and the risk factors for different groups were examined using multivariable-adjusted analyses.

Findings: Overall age-adjusted and sex-adjusted prevalence was estimated to be 6·0% (95% CI 5·8-6·3) for dementia, 3·9% (3·8-4·1) for Alzheimer's disease, 1·6% (1·5-1·7) for vascular dementia, and 0·5% (0·5-0·6) for other dementias. We estimated that 15·07 million (95% CI 14·53-15·62) people aged 60 years or older in China have dementia: 9·83 million (9·39-10·29) with Alzheimer's disease, 3·92 million (3·64-4·22) with vascular dementia, and 1·32 million (1·16-1·50) with other dementias. Overall MCI prevalence was estimated to be 15·5% (15·2-15·9), representing 38·77 million (37·95-39·62) people in China. Dementia and MCI shared similar risk factors including old age (dementia: odds ratios ranging from 2·69 [95% CI 2·43-2·98] to 6·60 [5·24-8·32]; MCI: from 1·89 [1·77-2·00] to 4·70 [3·77-5·87]); female sex (dementia: 1·43 [1·31-1·56]; MCI: 1·51 [1·43-1·59]); parental history of dementia (dementia: 7·20 [5·68-9·12]; MCI: 1·91 [1·48-2·46]); rural residence (dementia: 1·16 [1·06-1·27]; MCI: 1·45 [1·38-1·54]); fewer years of education (dementia: from 1·17 [1·06-1·29] to 1·55 [1·38-1·73]; MCI: from 1·48 [1·39-1·58] to 3·48 [3·25-3·73]); being widowed, divorced, or living alone (dementia: from 2·59 [2·30-2·90] to 2·66 [2·29-3·10]; MCI: from 1·58 [1·44-1·73] to 1·74 [1·56-1·95]); smoking (dementia: 1·85 [1·67-2·04]; MCI: 1·27 [1·19-1·36]), hypertension (dementia: 1·86 [1·70-2·03]; MCI: 1·62 [1·54-1·71] for MCI), hyperlipidaemia (dementia: 1·87 [1·71-2·05]; MCI: 1·29 [1·21-1·37]), diabetes (dementia: 2·14 [1·96-2·34]; MCI: 1·44 [1·35-1·53]), heart disease (dementia: 1·98 [1·73-2·26]; MCI: 1·17 [1·06-1·30]), and cerebrovascular disease (dementia: 5·44 [4·95-5·97]; MCI: 1·49 [1·36-1·62]). Nine of these risk factors are modifiable.

Interpretation: Dementia and MCI are highly prevalent in China and share similar risk factors. A prevention strategy should be developed to target the identified risk factors in the MCI population to thwart or slow down disease progression. It is also crucial to optimise the management of dementia and MCI as an important part of China's public health system.

Funding: Key Project of the National Natural Science Foundation of China, National Key Scientific Instrument and Equipment Development Project, Mission Program of Beijing Municipal Administration of Hospitals, Beijing Scholars Program, Beijing Brain Initiative from Beijing Municipal Science & Technology Commission, Project for Outstanding Doctor with Combined Ability of Western and Chinese Medicine, and Beijing Municipal Commission of Health and Family Planning.
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http://dx.doi.org/10.1016/S2468-2667(20)30185-7DOI Listing
December 2020

Effects of hyperbaric oxygen on NLRP3 inflammasome activation in the brain after carbon monoxide poisoning.

Undersea Hyperb Med 2020 Fourth Quarter;47(4):607-619

Department of Hyperbaric Oxygen, The Sixth Medical Center, PLA General Hospital, Beijing, China.

Neuroinflammation plays an important role in brain damage after acute carbon monoxide poisoning (ACOP). The nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing (NLRP) 3 inflammasome triggers the activation of inflammatory caspases and maturation of interleukin (IL)-1β and -18, and has been linked to various human autoinflammatory and autoimmune diseases. In this study we investigated the effects of hyperbaric oxygen (HBO2) on NLRP3 inflammasome activation after ACOP. Mice were randomly divided into four groups: sham group (exposure to normobaric air - i.e., 21% O2 at 1 atmosphere absolute); HBO2-only group; CO + normobaric air group; and CO + HBO2 group. Cognitive function was evaluated with the Morris water maze; myelin injury was assessed by FluoroMyelin GreenTM fluorescent myelin staining and myelin basic protein (MBP) immunostaining; and mRNA and protein levels of NLRP3 inflammasome complex proteins were measured by quantitative real-time PCR and Western blot, respectively. Additionally, serum and brain levels of IL-1ββ and -18 and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase were determined by enzyme-linked immunosorbent assay. It was found that HBO2 improved learning and memory, and alleviated myelin injury in mice subjected to acute CO exposure. Furthermore, HBO2 decreased NLRP3, absent in melanoma 2 (AIM2), caspase-1, and apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain mRNA and protein levels, and reduced brain and serum concentrations of IL-1β and -18 and NADPH oxidase. These results indicate that HBO2 suppresses the inflammatory response after ACOP by blocking NLRP3 inflammasome activation, thereby alleviating cognitive deficits.
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January 2021

Application of chromosome microarray analysis in prenatal diagnosis.

BMC Pregnancy Childbirth 2020 Nov 16;20(1):696. Epub 2020 Nov 16.

Department of Obstetrics, Weihai Maternal and Child Health Hospital, the Affiliated Weihai Second Municipal Hospital of Qingdao University, Weihai, China.

Background: To explore the application value of chromosomal microarray analysis (CMA) in prenatal diagnosis.

Methods: The results of chromosome karyotype analysis and CMA of 477 cases undergoing amniocentesis were analyzed. The results of the no ultrasound abnormality group and the ultrasound abnormality group were compared separately. Within the ultrasound abnormality group, the results of the ultrasound structural malformation group, the ultrasound soft index abnormality group, and other ultrasound abnormality (including abnormal amniotic fluid volume and fetal growth restriction) groups were compared.

Results: Abnormal chromosome and CMA results were found in a total of 71 cases (15.88%, 71/447), which can be broken down into a total of 23 karyotype abnormalities (5.15%, 23/447), consisting of 18 cases of aneuploidy (4.03%, 18/447), 2 cases of unbalanced chromosome rearrangements (0.44%, 2/447), and 3 cases of chimerism (0.67%, 3/447); 17 cases with detection of pathogenic copy number variations (pCNVs) (3.80%, 17/447); and 31 cases of detection of clinical variants of unknown significance (VOUS) (6.93%, 31/447). CMA detected 3.8% more genetic abnormalities than karyotype analysis (in addition to the abnormalities detected simultaneously by karyotype analysis). Between the no ultrasound abnormality group and the ultrasound abnormality group, there was an extremely significant difference in the detection rate of an abnormal chromosomal karyotype (P < 0.01) and of VOUS (P < 0.01), but there was no significant difference in the detection rate of pCNV (P > 0.05). Comparing the ultrasound structural malformation group, the ultrasound soft index abnormality group, and the other ultrasound abnormality group, there were no significant differences in the detection rate of abnormal chromosomal karyotypes (P > 0.05), pCNV (P > 0.05) or VOUS (P > 0.05).

Conclusions: The detection rate of chromosomal karyotype abnormalities in prenatal diagnosis in cases with no ultrasound abnormalities was higher. For cases with fetal ultrasound structural abnormalities, when compared with traditional karyotype analysis, CMA can improve the detection rate of fetal genetic abnormalities. However, the no ultrasound abnormality group also had a high VOUS abnormality detection rate, so it is necessary to strictly define the CMA indications.
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http://dx.doi.org/10.1186/s12884-020-03368-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667803PMC
November 2020

Integrated microarray analysis of key genes and a miRNA‑mRNA regulatory network of early‑onset preeclampsia.

Mol Med Rep 2020 Dec 30;22(6):4772-4782. Epub 2020 Sep 30.

Department of Obstetrics and Gynecology, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu 210004, P.R. China.

Early‑onset preeclampsia (EOPE) is a serious threat to maternal and foetal health. The present study aimed to identify potential biomarkers and targets for the treatment of EOPE. Expression profiles of placenta from patients with EOPE and healthy controls (GSE103542, GSE74341 and GSE44711) were downloaded from the Gene Expression Omnibus database. Integrated analysis revealed 246 genes and 28 microRNAs (miRNAs) that were differentially expressed between patients with EOPE and healthy controls. Differentially expressed genes (DEGs) were primarily enriched in 'biological processes', such as 'cell adhesion', 'female pregnancy', 'extracellular matrix organization' and 'response to hypoxia'. Significant pathways associated with DEGs primarily included 'focal adhesion', 'ECM‑receptor interaction', 'PI3K‑Akt signaling' and 'ovarian steroidogenesis'. A Protein‑Protein Interaction network of DEGs was constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins online database, and epidermal growth factor receptor, collagen α‑1(I) chain, secreted phosphoprotein 1, leptin (LEP), collagen α‑2(I) chain (COL1A2), plasminogen activator inhibitor 1 (SERPINE1), Thy‑1 membrane glycoprotein, bone morphogenetic protein 4, vascular cell adhesion protein 1 and matrix metallopeptidase 1 were identified as hub genes. The alterations of hsa‑miR‑937, hsa‑miR‑148b*, hsa‑miR‑3907, hsa‑miR‑367*, COL1A2, LEP and SERPINE1 in placenta were validated using our local samples. Our research showed that the expression of hsa‑miR‑937, hsa‑miR‑1486*, hsa‑miR‑3907, hsa‑miR‑367* and hub genes in the placenta were closely associated with the pathophysiology of EOPE. hsa‑miR‑937, hsa‑miR‑1486*, hsa‑miR‑3907, hsa‑miR‑367* and hub genes could serve as biomarkers for diagnosis and as potential targets for the treatment of EOPE.
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http://dx.doi.org/10.3892/mmr.2020.11551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646902PMC
December 2020

Coaxial spinning fabricated high nitrogen-doped porous carbon walnut anchored on carbon fibers as anodic material with boosted lithium storage performance.

J Colloid Interface Sci 2021 Mar 27;586:371-380. Epub 2020 Oct 27.

Key Laboratory of Energy Materials Chemistry, Ministry of Education; Key Laboratory of Advanced Functional Materials, Autonomous Region; Institute of Applied Chemistry, College of Chemistry, Xinjiang University, Urumqi, 830046, Xinjiang, PR China.

Commercial graphite with low theoretical capacity cannot meet the ever-increasing requirement demands of lithium-ion batteries (LIBs) caused by the rapid development of electric devices. Rationally designed carbon-based nanomaterials can provide a wide range of possibilities to meet the growing requirements of energy storage. Hence, the porous walnut anchored on carbon fibers with reasonable pore structure, N-self doping (10.2 at%) and novel structure and morphology is designed via interaction of inner layer polyethylene oxide and outer layer polyacrylonitrile and polyvinylpyrrolidone during pyrolysis of the obtained precursor, which is fabricated by coaxial electrospinning. As an electrode material, the as-made sample shows a high discharge capacity of 965.3 mA h g at 0.2 A g in the first cycle, retains a capacity of 819.7 mA h g after 500 cycles, and displays excellent cycling stability (475.2 mA h g at 1 A g after 1000 cycles). Moreover, the capacity of the electrode material still keeps 260.5 mA h g at 5 A g after 1000 cycles. Therefore, the obtained sample has a bright application prospect as a high performance anode material for LIBs. Besides, this design idea paves the way for situ N-enriched carbon material with novel structure and morphology by coaxial electrospinning.
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http://dx.doi.org/10.1016/j.jcis.2020.10.100DOI Listing
March 2021