Publications by authors named "Yan Long"

431 Publications

Long-Term Outcome of Enterprise Stenting for Symptomatic ICAS in a High-Volume Stroke Center.

Front Neurol 2021 17;12:672662. Epub 2021 Jun 17.

Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

The Enterprise stent has been used for treating intracranial atherosclerotic stenosis (ICAS), but its long-term outcome remains unclear. The purpose of this study was to evaluate the long-term clinical efficacy of the Enterprise stent used for patients with symptomatic ICAS due to hypoperfusion. Patients with symptomatic ICAS due to hypoperfusion treated with the Enterprise stents from a high-volume stroke center were evaluated. The successful recanalization was defined as the Modified Thrombolysis In Cerebral Infarction (mTICI) ≥ 2b. The stroke and neurological death that occurred within 72 h after the procedure as well as long-term clinical and imaging outcomes were analyzed. Overall, 130 patients with 130 ICAS treated with the Enterprise stent were included in our study. The successful recanalization rate was 100%. The mean pre- and postprocedural stenosis was 82.9 ± 8.9% vs. 15.1 ± 8.4%. Periprocedural complications occurred in 5 (3.8%) patients within 72 h after the procedure. Clinical follow-up data were available in 125 (96.2%) patients (median, 24 months) and any stroke or neurological death was encountered in 6 (4.8%) patients. Angiographic follow-up data was obtained from 118 (90.8%) patients (median, 13.5 months). In addition, 1-year in-stent restenosis (>70%) was found in 17 (14.4%) patients, and among them, 4 (23.5%) patients were symptomatic. Deployment of Enterprise stent is safe for ICAS. The short-term and long-term outcomes were acceptable, but the efficacy of the Enterprise stent needs to be further evaluated in future studies.
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http://dx.doi.org/10.3389/fneur.2021.672662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248485PMC
June 2021

Correlation between the positive rate of SAA in children with respiratory tract infection and ambient temperature.

Environ Sci Pollut Res Int 2021 Jun 24. Epub 2021 Jun 24.

Department of Clinical Laboratory, Guangzhou Women and Children's Medical Centre of Guangzhou Medical University, No. 9, Jinsui Road, Guangzhou, 510623, Guangdong, China.

The purpose of this study was to explore the application of serum amyloid A (SAA) in the outcome of upper respiratory tract infection in children by analyzing the correlation between the change of mean air temperature and the positive rate of SAA detection in children. Daily data on upper respiratory tract infection diseases and weather conditions were collected in 2016-2019. A quasi-Poisson regression with a distributed lag non-linear model was used to examine the association between temperature and SAA-positive rate. The positive rate of SAA had a moderate correlation with the temperature and a weak correlation with relative humidity. Low ambient temperature (7 °C, P) was related to the increase in the positive rate of SAA, with the effect lag for 0-7 days (RR 1.34 (1.19~1.74)). The increase in the SAA-positive case induced by 27 °C (P) could last for 0-14 days (RR 1.07 (1.01-1.08)), and high temperature (30 °C, P) could reduce the positive rate of SAA. Our findings add additional evidence to the adverse effects of sub-optimal ambient temperature and provide useful information for public health programs targeting pediatric patients.
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http://dx.doi.org/10.1007/s11356-021-15033-8DOI Listing
June 2021

Enhanced HSC-like cell generation from mouse pluripotent stem cells in a 3D induction system cocultured with stromal cells.

Stem Cell Res Ther 2021 06 19;12(1):353. Epub 2021 Jun 19.

Bone Marrow Transplantation Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, No.79 Qingchun Road, Hangzhou, Zhejiang, PR China.

Background: Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system.

Methods: In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. The in vivo hematopoietic reconstitution potential of c-kit cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system.

Results: The c-kit cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201c-kitcells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201c-kitcells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. Moreover, CD201 hematopoietic cells remained in a relatively slow cycling state, consistent with high expression levels of P57 and Ccng2. Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 hematopoietic cell induction from mouse PSCs.

Conclusions: Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application.
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http://dx.doi.org/10.1186/s13287-021-02434-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214308PMC
June 2021

CELL CYCLE SEITCH 52 regulates tillering by interacting with LATERAL SUPPRESSOR in non-heading Chinese cabbage.

Plant Sci 2021 Aug 3;309:110934. Epub 2021 May 3.

State Key Laboratory of Crop Genetics & Germplasm Enhancement, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of the P.R. China, China; Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of the P.R. China, Nanjing, 210095, China. Electronic address:

With the discovery of essential genes regulating tillering, such as MONOCULM 1 (MOC1) in rice and LATERAL SUPPRESSOR (LAS in Arabidopsis, LS in tomato), research on tillering mechanisms has made great progress; however, the study of tillering in non-heading Chinese cabbage (NHCC) is rare. Here, we report that BcLAS, as a member of the GRAS family, plays an important role in the tillering of NHCC during its vegetative growth. BcLAS was almost not expressed in other examed parts except leaf axils throughout life. When the expression of BcLAS was silenced utilizing virus-induced gene silencing (VIGS) technology, we found that the tiller number of 'Maertou' decreased sharply. In 'Suzhouqing', overexpression of BcLAS significantly promoted tillering. BcCCS52, the orthologue to CELL CYCLE SEITCH 52 (CCS52), interacts with BcLAS. Downregulation of the expression of BcCCS52 promoted tillering of 'Suzhouqing'; therefore, we conclude that BcCCS52 plays a negative role in tillering regulation. Our findings reveal the tillering regulation mechanism of NHCCs at the vegetative stage and report an orthologue of CCS52 regulating tillering in NHCC.
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http://dx.doi.org/10.1016/j.plantsci.2021.110934DOI Listing
August 2021

Early Prediction Model of Gestational Hypertension by Multi-Biomarkers Before 20 Weeks Gestation.

Diabetes Metab Syndr Obes 2021 31;14:2441-2451. Epub 2021 May 31.

Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, People's Republic of China.

Background: Gestational hypertension (GH), a hypertensive disorder of pregnancy (HDP), is a leading cause of maternal and fetal mortality due to the lack of clarity on its exact etiology and clinically feasible prediction models. This study was performed to discover novel biomarkers before 20 weeks gestation and thereby construct an early GH prediction model.

Methods: This study was designed based on differentially expressed protein screening followed by clinical validation. In the screening phase, a nested case-controlled study was conducted by plasma proteomic analyses using label-free LC-MS/MS and plasma samples from seven pre-GH cases before 20-week gestation and seven age- and gestational week-matched controls. In the validation phase, 10 proteins with differential expression in the screening phase were validated by ELISA or electrochemiluminescence in an independent study consisting of 29 pre-GH cases before 20-week gestation and 29 matched controls.

Results: In the screening phase, 149 proteins were found to be differentially expressed between the two groups and were predominantly involved in complement and coagulation cascades, platelet degranulation and positive regulation of cell motility. Further validation showed that serpin family C member 1 (SERPINC1), serpin family A member 5 (SERPINA5), complement factor H-related protein 5 (CFHR5), clusterin, cytokeratin 18 (CK18) and histidine-rich glycoprotein (HRG) levels were significantly higher in women who later developed GH compared to women with uncomplicated pregnancies (<0.05). Binary logistic regression analysis was used to determine the combination efficacy of models for early prediction of GH. The model with a combination of SERPINC1, CK18 and HRG had a significantly better discriminatory power (AUC = 0.91, 95% CI 0.83-0.98) compared to the models with those proteins alone as independent predictors of GH.

Conclusion: Plasma levels of SERPINC1, SERPINA5, CFHR5, clusterin, CK18 and HRG are potential novel predictive biomarkers of GH, and a prediction model using a combination of SERPINC1, CK18 and HRG has good discriminatory performance for GH before 20 weeks gestation.
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http://dx.doi.org/10.2147/DMSO.S309725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178612PMC
May 2021

Generation of a FTO gene knockout human embryonic stem cell line using CRISPR/Cas9 editing.

Stem Cell Res 2021 05 22;53:102362. Epub 2021 Apr 22.

Bone Marrow Transplantation Center, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, Hangzhou 310012, Zhejiang, PR China; Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou 311121, PR China. Electronic address:

Fat mass and obesity-associated protein (FTO) is the first protein found to have the activity of N6-methyladenosine (m6A) demethylation. It has been reported that FTO was involved in different physiological and pathological processes, including stem cell differentiation, sex determination, tumorigenesis, and progression. To further understand the exact role of FTO in these processes, we generated a FTO knockout human embryonic stem cell (hESC) line by CRISPR/Cas9 mediated gene editing method. This cell line maintained normal karyotype, pluripotency, and trilineage differentiation potential, which are considered as a model for function studies of the FTO protein in hESC self-renewal and differentiation.
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http://dx.doi.org/10.1016/j.scr.2021.102362DOI Listing
May 2021

Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system.

Stem Cell Res 2021 05 15;53:102350. Epub 2021 Apr 15.

BoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR China; Center of Stem Cell and Regenerative Medicine, School of Medicine, Zhejiang University, Hangzhou 310012, PR China. Electronic address:

Human ELTD1 (Epidermal growth factor, latrophilin and seven-transmembrane domain-containing 1), an orphan G-protein-coupled receptor (GPCR) belonging to the adhesion GPCR family, has been reported as a novel regulator of angiogenesis and a potential anti-cancer therapeutic target. However, little is known about the function of ELTD1, especially its undiscovered ligands. In this experiment, an ELTD1 homozygous knockout human embryonic stem cell line, FAHZUe001-A, was generated by the iCRISPR/Cas9 system to achieve a deeper understanding of ELTD1. The FAHZUe001-A was confirmed with normal karyotype, typical undifferentiated morphology, pluripotency and trilineage differentiation potential in vitro.
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http://dx.doi.org/10.1016/j.scr.2021.102350DOI Listing
May 2021

A feasible electrochemical biosensor for determination of glucose based on Prussian blue - Enzyme aggregates cascade catalytic system.

Bioelectrochemistry 2021 May 6;141:107838. Epub 2021 May 6.

The Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Northwestern Polytechnical University, Xi'an 710129, PR China.

The coral-like gold micro/nanostructures were formed onto carbon cloth followed by a Prussian blue (PB) electrochemical deposition to construct a highly sensitive HO biosensor. The SEM image of PB/Au/CC showed the coral-like gold morphology, and EDS and XPS tests also further confirmed the successful loading of Au and PB. The electrochemical tests of PB/Au/CC displayed the electrode possessed excellent performance in sensing HO, which was quantified in the linear range from 0.002 to 13.97 mM at an applied potential of -0.05 V, with a sensitivity of 454.97 μA mM cm and a detection limit of 0.5 μM (S/N = 3). And then a convenient sensing platform was established via the cross-linking enzyme aggregates method, using PB as the mediator to realize the construction of glucose BIOSENSOR [email protected]/Au/CC. The biosensor responded to glucose in the sensitivity of 70.76 μA mM cm within the linear range from 0.05 to 3.15 mM with a detection limit of 10 μM. The sensitivity was much higher than the electrode constructed by the cross-linking enzyme method ([email protected]/Au/CC), and it was also highly selective, reproducible, and stable. Besides, the proposed biosensor was successfully applied to the glucose determination in real human serum samples, which proved its practicability.
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http://dx.doi.org/10.1016/j.bioelechem.2021.107838DOI Listing
May 2021

Strong electrostatic adsorption-engaged fabrication of sub-3.0 nm PtRu alloy nanoparticles as synergistic electrocatalysts toward hydrogen evolution.

Nanoscale 2021 Jun 26;13(22):10044-10050. Epub 2021 May 26.

College of Chemistry and Materials Science, Sichuan Normal University, Chengdu 610068, China.

Alloying of Pt with Ru to form ultrafine and well-defined PtRu alloy nanoparticles (NPs) for synergistically electrocatalytic hydrogen evolution is highly desirable but remains a synthetic challenge. Here, we report a strong electrostatic adsorption (SEA)-assisted fabrication of ultrafine and homogeneously distributed PtRu alloy NPs using ethylenediaminetetraacetic acid tetrasodium-derived carbon (EC) as a matrix. The O, N-rich EC with a hierarchically macro/meso/microporous structure and the SEA-assisted formation of the [Ru(bpy)][PtCl] complex ensure the successful generation of ultrasmall PtRu alloy NPs (2.93 nm in diameter) with high dispersion. The optimal PtRu/EC-700 delivers excellent electrocatalytic properties with an ultralow overpotential (η = 18 mV), robust durability and good long-term stability for the alkaline hydrogen evolution reaction (HER). The ultrasmall PtRu alloy NPs with rich surface sites, the synergistic catalysis effect between Pt and Ru and the hierarchically macro/meso/microporous structure of O, N-rich EC cooperatively enhance the HER performance of PtRu/EC-700. This study provides an easy but effective way to construct metal alloy NPs with an ultrafine size and high dispersity for catalytic applications.
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http://dx.doi.org/10.1039/d1nr00936bDOI Listing
June 2021

Fabrication of uniform-aperture multi-focus microlens array by curving microfluid in the microholes with inclined walls.

Opt Express 2021 Apr;29(8):12763-12771

A variety of techniques have been proposed for fabricating high-density, high-numerical-aperture microlens arrays. However, a microlens array with a uniform focal length has a narrow depth of field, limiting the ability of depth perception. In this paper, we report on a fabrication method of multi-focus microlens arrays. The method for the preparation of the mold of the microlens array is based on 3D printing and microfluidic manipulation techniques. In the preparation of the mold, curved surfaces of the photo-curable resin with different curvatures are formed in the 3D printed microholes whose walls are inclined with different angles. The replicated microlens array consists of hundreds of lenslets with a uniform diameter of 500 µm and different focal lengths ranging from 635 µm to 970 µm. The multi-focus microlens array is capable of extending the depth of field for capturing clear images of objects at different distances ranging from 14.3 mm to 45.5 mm. The multi-focus microlens array has the potential to be used in a diversity of large-depth-of-field imaging and large-range depth perception applications.
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http://dx.doi.org/10.1364/OE.425333DOI Listing
April 2021

The tonoplast-localized transporter OsABCC9 is involved in cadmium tolerance and accumulation in rice.

Plant Sci 2021 Jun 26;307:110894. Epub 2021 Mar 26.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, 530004, China. Electronic address:

Cadmium (Cd) is a highly toxic element to living organisms, and its accumulation in the edible portions of crops poses a potential threat for human health. The molecular mechanisms underlying Cd detoxification and accumulation are not fully understood in plants. In this study, the involvement of a C-type ABC transporter, OsABCC9, in Cd tolerance and accumulation in rice was investigated. The expression of OsABCC9 was rapidly induced by Cd treatment in a concentration-dependent manner in the root. The transporter, localized on the tonoplast, was mainly expressed in the root stele under Cd stress. OsABCC9 knockout mutants were more sensitive to Cd and accumulated more Cd in both the root and shoot compared to the wild-type. Moreover, the Cd concentrations in the xylem sap and grain were also significantly increased in the knockout lines, suggesting that more Cd was distributed from root to shoot and grain in the mutants. Heterologous expression of OsABCC9 in yeast enhanced Cd tolerance along with an increase of intracellular Cd content. Taken together, these results indicated that OsABCC9 mediates Cd tolerance and accumulation through sequestration of Cd into the root vacuoles in rice.
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http://dx.doi.org/10.1016/j.plantsci.2021.110894DOI Listing
June 2021

Proteome of Saccharomyces cerevisiae under paraquat stress regulated by therapeutic concentration of copper ions.

Ecotoxicol Environ Saf 2021 Jul 21;217:112245. Epub 2021 Apr 21.

Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou 510632, China.

Paraquat (PQ) is a non-selective herbicide with strong toxicity to humans and mammals. However, the proteome regulation of cells by PQ is still unclear, limiting the development of effective antidotes. Studies have shown that a slight excess of intracellular copper levels could be beneficial to the survival under exposure to PQ. In this study, Saccharomyces cerevisiae was used as a model to explore the regulation effect of copper ions on PQ poisoning by the approach of date independent acquisition proteomics. The results showed that toxic effect of PQ was primarily induced by oxidative damage in the mitochondria and the disorder of gene expression. The addition of Cu involved a series of favorable reactions to cell survival under PQ stress, including activation of the mitogen-activated protein kinase signaling pathway, regulation of processes such as sulfur metabolism, carbon metabolism and gene expression in cells. The generation of glutathione, heme and steroids advantageous to cell growth under stress was also increased. These findings inferred that therapeutic concentration of copper ions could prolong the survival of cells under PQ stress.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112245DOI Listing
July 2021

Genome-wide identification and functional characterization of LEA genes during seed development process in linseed flax (Linum usitatissimum L.).

BMC Plant Biol 2021 Apr 21;21(1):193. Epub 2021 Apr 21.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Background: LEA proteins are widely distributed in the plant and animal kingdoms, as well as in micro-organisms. LEA genes make up a large family and function in plant protection against a variety of adverse conditions.

Results: Bioinformatics approaches were adopted to identify LEA genes in the flax genome. In total, we found 50 LEA genes in the genome. We also conducted analyses of the physicochemical parameters and subcellular location of the genes and generated a phylogenetic tree. LuLEA genes were unevenly mapped among 15 flax chromosomes and 90% of the genes had less than two introns. Expression profiles of LuLEA showed that most LuLEA genes were expressed at a late stage of seed development. Functionally, the LuLEA1 gene reduced seed size and fatty acid contents in LuLEA1-overexpressed transgenic Arabidopsis lines.

Conclusion: Our study adds valuable knowledge about LEA genes in flax which can be used to improve related genes of seed development.
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http://dx.doi.org/10.1186/s12870-021-02972-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059249PMC
April 2021

Serum complement proteomics reveal biomarkers for hypertension disorder of pregnancy and the potential role of Clusterin.

Reprod Biol Endocrinol 2021 Apr 19;19(1):56. Epub 2021 Apr 19.

Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, No.9, Jinsui Road, Guangzhou, 510623, Guangdong, China.

Introduction: Hypertension disorder of pregnancy (HDP) is one of the leading causes of maternal and foetal illness. The aim of the current study was to identify and verify novel serum markers for HDP.

Methods: A label-free LC-MS/MS method was used to establish the serum proteomic profiles of 12 pre-HDP (before clinical diagnosis of HDP) pregnancies and verify prioritized candidates in the verification set of 48 pre-HDP pregnancies. These biomarkers were revalidated by ELISA in an independent cohort of 88 pre-HDP pregnancies. Subsequently, the candidate biomarkers were histologically analysed by immunohistochemistry, and function was evaluated in TEV-1 cells.

Results: We identified 33 proteins with significantly increased abundance and 14 with decreased abundance (peptide FDR ≤ 1%, P < 0.05). Complement was one of the top enriched components in the pre-HDP group compared with the control group. Three complement factors (CLU, CFHR5, and CRP) were significantly increased in the three sets, of which CLU was a critical factor for the development of HDP (OR = 1.22, P < 0.001). When these three factors and body weight were combined, the AUC was 0.74, with a sensitivity of 0.67 and specificity of 0.68 for HDP prediction compared with normal pregnancy. In addition, inflammation-induced CLU could inhibit the invasion of TEV-1 cells.

Conclusions: Complement proteins may play an essential role in the occurrence of HDP by acting on trophoblast cells. CLU may be a high-risk factor for HDP, and the models combining candidates show reasonable screening efficiency of HDP in the first half of pregnancy.
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http://dx.doi.org/10.1186/s12958-021-00742-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054419PMC
April 2021

Inhibits Branching of Non-Heading Chinese Cabbage at the Vegetative Stage.

Plants (Basel) 2021 Mar 9;10(3). Epub 2021 Mar 9.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture, Nanjing 210095, China.

Branching is speculated to contribute to the plant architecture and crop yield. As a quantitative trait, branching is regulated by multiple genes in non-heading Chinese cabbage (NHCC). Several related candidate genes have been discovered in previous studies on the branching of NHCC, but their specific functions and regulatory mechanisms still need to be verified and explored. In this study, we found that the expression of , the ortholog to in , was significantly different between 'Suzhouqing' (common type) and 'Maertou' (multiple shoot branching type) in NHCC, which was consistent with the previous transcriptome sequencing results. The silencing of expression in non-heading Chinese cabbage promotes axillary bud growth at the vegetative stage. When is overexpressed in , branching will decrease. In further study, we found that interacts with immunophilin in vivo and in vitro through yeast two-hybrid analysis and bimolecular fluorescence complementation (BiFC) assays. Moreover, quantitative real-time PCR analysis showed that when the expression of was silenced in 'Suzhouqing', the expression of also decreased significantly. Our findings reveal that is involved in the branching mechanism and interacts with immunophilin in NHCC.
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http://dx.doi.org/10.3390/plants10030510DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999546PMC
March 2021

High-Throughput Cell Trapping in the Dentate Spiral Microfluidic Channel.

Micromachines (Basel) 2021 Mar 9;12(3). Epub 2021 Mar 9.

Engineering Research Center of Optical Instrument and System, the Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China.

Cell trapping is a very useful technique in a variety of cell-based assays and cellular research fields. It requires a high-throughput, high-efficiency operation to isolate cells of interest and immobilize the captured cells at specific positions. In this study, a dentate spiral microfluidic structure is proposed for cell trapping. The structure consists of a main spiral channel connecting an inlet and an out and a large number of dentate traps on the side of the channel. The density of the traps is high. When a cell comes across an empty trap, the cell suddenly makes a turn and enters the trap. Once the trap captures enough cells, the trap becomes closed and the following cells pass by the trap. The microfluidic structure is optimized based on the investigation of the influence over the flow. In the demonstration, 4T1 mouse breast cancer cells injected into the chip can be efficiently captured and isolated in the different traps. The cell trapping operates at a very high flow rate (40 μL/s) and a high trapping efficiency (>90%) can be achieved. The proposed high-throughput cell-trapping technique can be adopted in the many applications, including rapid microfluidic cell-based assays and isolation of rare circulating tumor cells from a large volume of blood sample.
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http://dx.doi.org/10.3390/mi12030288DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000121PMC
March 2021

The complete mitochondrial genome of (Scorpaeniformes: Triglidae) from Jiangsu Province, China.

Mitochondrial DNA B Resour 2021 Mar 15;6(3):853-854. Epub 2021 Mar 15.

Jiangsu Marine Fisheries Research Institute, Nantong, People's Republic of China.

In this study, we present the complete mitogenome and a phylogenetic analysis of characterized using Illumina next-generation and Pacific Biosciences (PacBio, Menlo Park, CA) sequencing technologies. The complete mitochondrial genome is a circular molecule 16,511 bp in length and contains the same set of 37 mitochondrial genes (13 protein-coding genes, two ribosomal RNA (rRNA), 22 transfer RNA (tRNA)), and a control region as other bony fishes. The base composition of the entire mitogenome showed a slight AT bias. Phylogenetic analysis of the mitogenome of fully resolved it in a clade with other species classified to the Triglidae. The mitogenome data produced in this study provide the genomic resources available for future evolutionary studies.
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http://dx.doi.org/10.1080/23802359.2021.1885317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971344PMC
March 2021

Altered follicular regulatory T (Tfr)- and helper T (Tfh)-cell subsets are associated with autoantibody levels in microscopic polyangiitis patients.

Eur J Immunol 2021 Jul 23;51(7):1809-1823. Epub 2021 Apr 23.

Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.

Antineutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV) is an autoimmune disease characterized by B cells-derived ANCAs, and ANCA was proved to be a key factor in its pathogenesis. Follicular regulatory T (Tfr) and follicular helper T (Tfh) cells were T-cell subsets that play important roles in B-cell maturation and antibody production. However, their significances in microscopic polyangiitis (MPA) patients, one type of AAV, has not been thoroughly studied. In this study, comprehensive pattern analyses of circulating Tfr and Tfh were performed in MPA patients and healthy controls (HCs), and we found Tfr levels and Tfr/Tfh ratios were significantly decreased in MPA patients. Compared with HCs, Helios+, CD45RA-FoxP3hi, and Ki-67+ Tfr were lower in MPA patients, while CD226+ Tfr cells were higher. These phenotypes suggest that function and proliferation ability of Tfr cells were relatively impaired. Tfh subsets, including ICOS+PD-1+ and Ki-67+ Tfh, were significantly increased, suggesting that the function of Tfh was enhanced in MPA although the total Tfh levels did not change significantly. Circulating memory B cells and plasmablasts were significantly elevated and negatively correlated with Tfr levels and Tfr/Tfh ratios in MPA patients. In addition, Tfr levels and Tfr/Tfh ratios were negatively while Tfh was positively correlated with serum myeloperoxidase (MPO)-ANCA levels. Furthermore, Tfr and Tfr/Tfh ratio were also reversely associated with SCr, BUN, IL-4, and IL-21 levels. Our results suggest that the imbalance of Tfr and Tfh functional subsets is related to increased level of autoantibodies in MPA patients, and we propose a new mechanism for the pathogenesis of MPA.
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http://dx.doi.org/10.1002/eji.202049093DOI Listing
July 2021

Sustainable one-pot construction of oxygen-rich nitrogen-doped carbon nanosheets stabilized ultrafine Rh nanoparticles for efficient ammonia borane hydrolysis.

J Colloid Interface Sci 2021 Jul 10;594:131-140. Epub 2021 Mar 10.

College of Chemistry and Materials Science, Sichuan Normal University, Chengdu 610068, China. Electronic address:

Heteroatom-doped porous carbons that possess large surface areas and well-defined porosity show great promise in heterogeneous catalysis, whereas their syntheses inevitably require complicated steps, hazardous activation and functional reagents, and an inert gas atmosphere. Herein, a one-pot synthetic strategy to oxygen-rich porous nitrogen-doped carbon (OPNC) is developed through pyrolysis of ethylenediamine tetra-acetic acid tetra-sodium in air without any activation and functionalization agents. The as-prepared OPNC with more surface oxygenated groups and mesopores not only benefits synthesis of well-dispersed ultrafine Rh nanoparticles (NPs) with abundant accessible active sites, but also facilitates the diffusion of reactants and avoids mass transfer limitations, thereby considerably contributes to a high performance toward AB hydrolysis. Specifically, the optimal Rh/OPNC exhibits a high activity toward AB hydrolysis with a turnover frequency (TOF) of 433 min. The kinetic isotope studies indicate that the cleavage of OH bond in HO molecules is the rate-determining step (RDS). The Rh/OPNC can be reused for five repetitive cycles with approximately 62% remained activity of the first cycle. The catalytic activity of Rh/OPNC can be further improved with a very high TOF of 1201 min in alkaline solution. This study proposes a simple and sustainable pathway to synthesize efficient catalyst support for depositing metal NPs toward AB hydrolysis.
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http://dx.doi.org/10.1016/j.jcis.2021.02.086DOI Listing
July 2021

Characterization of the Common Genetic Basis Underlying Seed Hilum Size, Yield, and Quality Traits in Soybean.

Front Plant Sci 2021 25;12:610214. Epub 2021 Feb 25.

Root Biology Center, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, China.

Developing high yielding cultivars with outstanding quality traits are perpetual objectives throughout crop breeding operations. Confoundingly, both of these breeding objectives typically involve working with complex quantitative traits that can be affected by genetic and environmental factors. Establishing correlations of these complex traits with more easily identifiable and highly heritable traits can simplify breeding processes. In this study, two parental soybean genotypes contrasting in seed hilum size, yield, and seed quality, as well as 175 F recombinant inbred lines (RILs) derived from these parents, were grown in 3 years. The of four hilum size, two quality and two yield traits, ranged from 0.72 to 0.87. The four observed hilum size traits exhibited significant correlation ( < 0.05) with most of seed yield and quality traits, as indicated by correlation coefficients varying from -0.35 to 0.42, which suggests that hilum size could be considered as a proxy trait for soybean yield and quality. Interestingly, among 53 significant quantitative trait loci (QTLs) with logarithm of odds (LOD) values ranging from 2.51 to 6.69 and accounting for 6.40-16.10% of genetic variation, three loci encoding hilum size, , , and , colocated with QTLs for seed yield and quality traits, demonstrating that genes impacting seed hilum size colocalize in part with genes acting on soybean yield and quality. As a result of the breeding efforts and field observations described in this work, it is reasonable to conclude that optimizing hilum size through selection focused on a few QTLs may be useful for breeding new high yielding soybean varieties with favorable quality characteristics.
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http://dx.doi.org/10.3389/fpls.2021.610214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947287PMC
February 2021

Gut Microbiota Signatures in Gestational Anemia.

Front Cell Infect Microbiol 2021 25;11:549678. Epub 2021 Feb 25.

Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, China.

Gestational diseases are associated with altered intestinal microbiota in pregnant women. Characterizing the gut microbiota of gestational anemia (GA) may describe a novel role of gut microbial abnormality in GA. In this study, we investigated differences in gut microbiota between GA patients and healthy pregnant women from the first trimester (n = 24 54) and the third trimester (n = 30 56) based on the 16S rRNA gene sequencing method. No statistically significant differences in α-diversity were identified between GA patients and controls in the first trimester of pregnancy, whereas the Shannon index and observed OTUs were significantly lower in GA patients than in healthy controls in the third trimester. Distance-based redundancy analysis revealed striking differences in microbial communities in the third trimester between GA patients and controls. Four genera were significantly different in relative abundance between GA patients and healthy controls, while 12 genera differentiated significantly between GA patients and healthy controls in the third trimester. At the operational taxonomic unit (OTU) level, 17 OTUs and 30 OTUs were identified to be different between GA patients and healthy controls in the first and third trimesters, respectively. Changes in gut microbial composition of GA patients suggest a potential relation with GA, and provide insights into the prediction and intervention of gestational anemia.
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http://dx.doi.org/10.3389/fcimb.2021.549678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947918PMC
June 2021

Identification of lncRNAs associated with the pathogenesis of ankylosing spondylitis.

BMC Musculoskelet Disord 2021 Mar 12;22(1):272. Epub 2021 Mar 12.

Department of Rheumatology, First Affiliated Hospital of Anhui University of Chinese Medicine, No 117 Meishan Road, Shushan District, Hefei City, Anhui Province, 230031, People's Republic of China.

Background: Ankylosing spondylitis (AS) is a chronic autoimmune disease affecting the sacroiliac joint. To date, few studies have examined the association between long non-coding RNAs (lncRNAs) and AS pathogenesis. As such, we herein sought to characterize patterns of AS-related lncRNA expression and to evaluate the potential role played by these lncRNAs in this complex autoimmune context.

Methods: We conducted a RNA-seq analysis of peripheral blood mononuclear cell (PBMC) samples isolated from five AS patients and corresponding controls. These data were then leveraged to characterize AS-related lncRNA expression patterns. We further conducted GO and KEGG enrichment analyses of the parental genes encoding these lncRNAs, and we confirmed the validity of our RNA-seq data by assessing the expression of six lncRNAs via qRT-PCR in 15 AS and control patient samples. Pearson correlation analyses were additionally employed to examine the associations between the expression levels of these six lncRNAs and patient clinical index values.

Results: We detected 56,575 total lncRNAs in AS and control patient samples during our initial RNA-seq analysis, of which 200 and 70 were found to be up- and down-regulated (FC > 2 or < 0.05; P < 0.05), respectively, in AS samples relative to controls. In qRT-PCR validation assays, we confirmed the significant upregulation of NONHSAT118801.2, ENST00000444046, and NONHSAT183847.1 and the significant downregulation of NONHSAT205110.1, NONHSAT105444.2, and NONHSAT051856.2 in AS patient samples. We further found the expression of NONHSAT118801.2 and NONHSAT183847.1 to be positively correlated with disease severity.

Conclusion: Overall, our findings highlight several lncRNAs that are specifically expressed in PBMCs of AS patients, indicating that they may play key functions in the pathogenesis of this autoimmune disease. Specifically, we determined that NONHSAT118801.2 and NONHSAT183847.1 may influence the occurrence and development of AS.
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http://dx.doi.org/10.1186/s12891-021-04119-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955637PMC
March 2021

Enhanced tolerance to drought stress resulting from Caragana korshinskii CkWRKY33 in transgenic Arabidopsis thaliana.

BMC Genom Data 2021 03 10;22(1):11. Epub 2021 Mar 10.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Background: It is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses.

Results: In this study, a WRKY transcription factor, WRKY33, was cloned from Caragana korshinskii. A sequence structure analysis showed that it belonged to the Group-I type. Subcellular localization experiments in tobacco epidermal cells showed the presence of CkWRKY33 in the nucleus. Additionally, CkWRKY33 was overexpressed in Arabidopsis thaliana. A phenotypic investigation revealed that compared with wild-type plants, CkWRKY33-overexpressing transgenic plants had higher survival rates, as well as relative soluble sugar, proline and peroxidase contents, but lower malondialdehyde contents, following a drought stress treatment.

Conclusions: This suggested that the overexpression of CkWRKY33 led to an enhanced drought-stress tolerance in transgenic A. thaliana. Thus, CkWRKY33 may act as a positive regulator involved in the drought-stress responses in Caragana korshinskii.
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http://dx.doi.org/10.1186/s12863-021-00965-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7945665PMC
March 2021

Increased circulating PD-1CXCR5- peripheral helper T cells are associated with disease severity of active ulcerative colitis patients.

Immunol Lett 2021 May 3;233:2-10. Epub 2021 Mar 3.

Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China. Electronic address:

Purpose: A recently identified population of T cells, phenotypically CD4PD-1CXCR5, has been firstly termed as peripheral helper T cells (Tph) and found to be pathogenic in autoimmune diseases. However, the potential role of Tph in ulcerative colitis (UC) remains unclear. We aim to investigate the changes of circulating Tph in UC patients and their potential significance in the pathogenesis of UC.

Methods: Totally 68 UC patients and 34 age- and sex-matched healthy controls were enrolled. Circulating Tph and B cell subsets were analyzed by flow cytometry. Expressions of inducible T-cell co-stimulator (ICOS) on Tph cells were analyzed. Serum IL-4, IL-10, IL-12 and IL-21 were detected using ELISA. Correlation analyses were conducted between Tph cells and disease severity, functional B cell subsets and serum cytokines.

Results: Both the frequency and absolute number of Tph were significantly increased in active UC patients and ICOS levels in Tph cells were also elevated, compared with remission UC patients and healthy controls. Tph and ICOS expression were significantly positively correlated with Mayo score and serum CRP in active UC patients, and were significantly decreased when achieving remission after treatment. Tph levels were correlated with new memory B cells, plasmablasts, serum IL-4 and IL-21. Meanwhile, serum IL-10 showed negative correlation while IL-12 exhibited positive correlation with circulating Tph cells in UC patients.

Conclusions: Circulating Tph cells are elevated in active UC patients and are associated with the disease activity, which may contribute to the pathogenesis of UC.
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http://dx.doi.org/10.1016/j.imlet.2021.03.001DOI Listing
May 2021

Effects of Bicultural Identity Integration and National Identity on COVID-19-Related Anxiety Among Ethnic Minority College Students: The Mediation Role of Power Values.

Psychol Res Behav Manag 2021 24;14:239-249. Epub 2021 Feb 24.

Faculty of Psychology, Southwest University, Chongqing, People's Republic of China.

Purpose: The current study investigated the association between bicultural identity integration (BII, incorporating BII-harmony and BII-blendedness), national identity, and anxiety related to Coronavirus disease 2019 (COVID-19) among ethnic minority college students. In addition, this research examined the mediation role of power values in the relationship between BII, national identity, and COVID-19-related anxiety.

Methods: This cross-sectional research design made use of online surveys. Using convenience sampling, participants comprised 235 Chinese ethnic minority college students drawn from four colleges in the ethnic minority autonomous regions of China. Data were collected during June 2020. Participants mainly lived in ethnic minority communities or villages in southwest China before receiving higher education at urban campuses.

Results: Correlation analysis revealed that BII-harmony, BII-blendedness, and national identity were significantly negatively correlated with COVID-19-related anxiety. Mediation model analysis showed that power values were significantly positively correlated with COVID-19-related anxiety. Power values play a mediating role in the relationship between BII-harmony, national identity, and COVID-19-related anxiety, and have an inhibitory effect on this relationship.

Conclusion: Our findings indicate that BII-harmony and national identity could have the function of protecting ethnic minority college students from COVID-19-related anxiety. Emphasizing individualistic personal power values could increase COVID-19-related anxiety, whereas a collectivist identity reduces anxiety. These findings could provide another perspective on psychological interventions to reduce anxiety during the COVID-19 pandemic.
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http://dx.doi.org/10.2147/PRBM.S294547DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917360PMC
February 2021

CRISPR/Cas9-mediated CysLT1R deletion reverses synaptic failure, amyloidosis and cognitive impairment in APP/PS1 mice.

Aging (Albany NY) 2021 02 11;13(5):6634-6661. Epub 2021 Feb 11.

Department of Pharmacology, China Pharmaceutical University, Nanjing, China.

As a major pathological hallmark of Alzheimer's disease (AD), amyloid-β (Aβ) is regarded as a causative factor for cognitive impairment. Extensive studies have found Aβ induces a series of pathophysiological responses, finally leading to memory loss in AD. Our previous results demonstrated that cysteinyl leukotrienes receptor 1 (CysLTR) antagonists improved exogenous Aβ-induced memory impairment. But the role of CysLTR in AD and its underlying mechanisms still remain elusive. In this study, we investigated CysLTR levels in AD patients and APP/PS1 mice. We also generated APP/PS1-CysLTR mice by clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated CysLTR deletion in APP/PS1 mice and studied the effect of CysLTR knockout on amyloidogenesis, synapse structure and plasticity, cognition, neuroinflammation, and kynurenine pathway. These attributes were also studied after lentivirus-mediated knockdown of CysLTR gene in APP/PS1 mice. We found that CysLTR knockout or knockdown could conserve synaptic structure and plasticity, and improve cognition in APP/PS1 mice. These effects were associated with concurrent decreases in amyloid processing, reduced neuroinflammation and suppression of the kynurenine pathway. Our study demonstrates that CysLTR deficiency can mediate several beneficial effects against AD pathogenesis, and genetic/pharmacological ablation of this protein could be a potential therapeutic option for AD.
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http://dx.doi.org/10.18632/aging.202501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993729PMC
February 2021

Takeda G Protein-Coupled Receptor 5 Modulates Depression-like Behaviors via Hippocampal CA3 Pyramidal Neurons Afferent to Dorsolateral Septum.

Biol Psychiatry 2021 06 25;89(11):1084-1095. Epub 2020 Nov 25.

Department of Pharmacology, Key Laboratory of Neuropsychiatric Diseases, China Pharmaceutical University, Nanjing, China. Electronic address:

Background: Takeda G protein-coupled receptor 5 (TGR5) is recognized as a promising target for type 2 diabetes and metabolic syndrome; its expression has been demonstrated in the brain and is thought to be neuroprotective. Here, we hypothesize that dysfunction of central TGR5 may contribute to the pathogenesis of depression.

Methods: In well-established chronic social defeat stress (CSDS) and chronic restraint stress (CRS) models of depression, we investigated the functional roles of TGR5 in CA3 pyramidal neurons (PyNs) and underlying mechanisms of the neuronal circuit in depression (for in vivo studies, n = 10; for in vitro studies, n = 5-10) using fiber photometry; optogenetic, chemogenetic, pharmacological, and molecular profiling techniques; and behavioral tests.

Results: Both CSDS and CRS most significantly reduced TGR5 expression of hippocampal CA3 PyNs. Genetic overexpression of TGR5 in CA3 PyNs or intra-CA3 infusion of INT-777, a specific agonist, protected against CSDS and CRS, exerting significant antidepressant-like effects that were mediated via CA3 PyN activation. Conversely, genetic knockout or TGR5 knockdown in CA3 facilitated stress-induced depression-like behaviors. Re-expression of TGR5 in CA3 PyNs rather than infusion of INT-777 significantly improved depression-like behaviors in Tgr5 knockout mice exposed to CSDS or CRS. Silencing and stimulation of CA3 PyNs→somatostatin-GABAergic (gamma-aminobutyric acidergic) neurons of the dorsolateral septum circuit bidirectionally regulated depression-like behaviors, and blockade of this circuit abrogated the antidepressant-like effects from TGR5 activation of CA3 PyNs.

Conclusions: These findings indicate that TGR5 can regulate depression via CA3 PyNs→somatostatin-GABAergic neurons of dorsolateral septum transmission, suggesting that TGR5 could be a novel target for developing antidepressants.
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http://dx.doi.org/10.1016/j.biopsych.2020.11.018DOI Listing
June 2021

Network analysis and transcriptome profiling in peripheral blood mononuclear cells of patients with rheumatoid arthritis.

Exp Ther Med 2021 Feb 25;21(2):170. Epub 2020 Dec 25.

Laboratory for Rheumatism, Institute of Rheumatology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui 230011, P.R. China.

The present study aimed to investigate the differential expression of long non-coding RNAs (lncRNAs) in rheumatoid arthritis (RA). High-throughput gene sequencing technology was used to detect the expression of lncRNA and mRNA in three patients with RA (RA group) and normal controls (NC group). A Bioinformatics analysis was used to assess the effects of differentially expressed mRNAs on signaling pathways and biological functions. The selected dysregulated lncRNAs were verified by reverse transcription-quantitative (RT-q)PCR in the peripheral blood mononuclear cells (PBMCs) of patients with RA and age- and sex-matched controls. A correlation analysis was used to analyze the relationship between lncRNAs and clinical indexes. From the lncRNA sequencing data, significantly differentially expressed lncRNAs between the RA and NC groups were identified by a fold change ≥2 and P<0.05. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis suggested that the differentially expressed mRNAs were mainly involved in organelle composition, intracellular regulation, signaling pathways, cancer, virus and inflammation. A total of four of these lncRNAs were confirmed by RT-qPCR to be significantly differentially expressed (LINC00304, MIR503HG, LINC01504 and FAM95B1). Through the correlation analysis, it was confirmed that there was a strong correlation between these lncRNAs and clinical laboratory indicators and indexes such as course of disease, arthrocele and joint tenderness. Overall, the present results suggested that the expression levels of LINC00304, MIR503HG, LINC01504 and FAM95B1 in PBMCs from patients with RA may serve as potential biomarkers for RA diagnosis, influencing the occurrence and progress of RA.
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http://dx.doi.org/10.3892/etm.2020.9601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792483PMC
February 2021

[Differentially expressed inflammatory proteins in acute gouty arthritis based on protein chip].

Zhejiang Da Xue Xue Bao Yi Xue Ban 2020 Dec;49(6):743-749

Department of Rheumatology and Immunology, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230031, China.

Objective: To detect the differentially expressed inflammatory proteins in acute gouty arthritis (AGA) with protein chip.

Methods: The Raybiotech cytokine antibody chip was used to screen the proteomic expression in serum samples of 10 AGA patients and 10 healthy individuals. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were applied to determine the biological function annotation of differentially expressed proteins and the enrichment of signal pathways. ELISA method was used to verify the differential protein expression in 60 AGA patients and 60 healthy subjects. The ROC curve was employed to evaluate the diagnostic value of differential proteins in AGA patients.

Results: According to|logFC|>log 1.2 and corrected <0.01, 4 most differentially expressed proteins in AGA patients were identified, including tumor necrosis factor receptor super family members Ⅱ (TNF RⅡ), macrophage inflammatory protein 1β (MIP-1β), interleukin-8 (IL-8), and granulocyte-macrophage colony stimulating factor (GM-CSF). GO and KEGG enrichment analysis showed that the differentially expressed proteins were related to inflammation, metabolism and cytokine pathways. The ELISA results showed that serum levels of differentially expressed proteins were significantly different between AGA patients and healthy subjects(all <0.01). ROC curve analysis showed that the areas under the curve (AUCs) of GM-CSF, IL-8, MIP-1β and TNF RⅡ for predicting AGA were 0.657 (95% : 0.560-0.760, sensitivity: 68.33%, specificity: 50.00%), 0.994 (95% : 0.980-1.000, sensitivity: 100.00%, specificity: 61.67%), 0.980 (95% : 0.712-0.985, sensitivity: 95.00%, specificity: 98.33%) and 0.965 (95% : 0.928-1.000, sensitivity: 100.00%, specificity: 10.00%), respectively.

Conclusions: Proteomics can be applied to identify the biomarkers of AGA, which may be used for risk prediction and diagnosis of AGA patients.
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http://dx.doi.org/10.3785/j.issn.1008-9292.2020.12.09DOI Listing
December 2020

Active Fault Localization of Actuators on Torpedo-Shaped Autonomous Underwater Vehicles.

Sensors (Basel) 2021 Jan 11;21(2). Epub 2021 Jan 11.

School of Mechatronic Engineering and Automation, Shanghai University, Shanghai 200444, China.

To ensure the mission implementation of Autonomous Underwater Vehicles (AUVs), faults occurring on actuators should be detected and located promptly; therefore, reliable control strategies and inputs can be effectively provided. In this paper, faults occurring on the propulsion and attitude control systems of a torpedo-shaped AUV are analyzed and located while fault features may induce confusions for conventional fault localization (FL). Selective features of defined fault parameters are assorted as necessary conditions against different faulty actuators and synthesized in a fault tree subsequently to state the sufficiency towards possible abnormal parts. By matching fault features with those of estimated fault parameters, suspected faulty sections are located. Thereafter, active FL strategies that analyze the related fault parameters after executing purposive actuator control are proposed to provide precise fault location. Moreover, the generality of the proposed methods is analyzed to support extensive implementations. Simulations based on finite element analysis against a torpedo-shaped AUV with actuator faults are carried out to illustrate the effectiveness of the proposed methods.
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http://dx.doi.org/10.3390/s21020476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827271PMC
January 2021