Publications by authors named "Yan Li"

14,424 Publications

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Corrigendum to "Effects of PET microplastics on the physiology of Drosophila" [Chemosphere 283 (2021) 131289].

Chemosphere 2022 May 23;303(Pt 1):134678. Epub 2022 May 23.

College of Automation, Hangzhou Dianzi University, Hangzhou, 310018, China.

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http://dx.doi.org/10.1016/j.chemosphere.2022.134678DOI Listing
May 2022

Association of Nighttime Masked Uncontrolled Hypertension With Left Ventricular Hypertrophy and Kidney Function Among Patients with Chronic Kidney Disease Not Receiving Dialysis.

JAMA Netw Open 2022 May 2;5(5):e2214460. Epub 2022 May 2.

Department of Nephrology, Institute of Nephrology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.

Importance: Nighttime hypertension is prevalent and associated with adverse outcomes in patients with chronic kidney disease (CKD), but nighttime hypertension, a subtype of masked uncontrolled hypertension (MUCH), is often undetected among patients with controlled office blood pressure. Little attention has been paid to patients with CKD and nighttime MUCH.

Objective: To investigate the prevalence of nighttime MUCH and its associations with cardiovascular and kidney outcomes in patients with CKD who were not receiving dialysis.

Design, Setting, And Participants: This retrospective cohort study included patients with nondialysis CKD and hypertension, enrolled in Shanghai, China, from July 2012 through November 2020 and followed up for a median of 39 months.

Exposures: Participants were classified as having controlled hypertension, sustained hypertension, and MUCH, which was further divided into isolated nighttime MUCH and day-night MUCH, assessed by office and ambulatory blood pressure monitoring.

Main Outcomes And Measures: Left ventricular hypertrophy (LVH) was determined by echocardiography. The composite kidney outcome consisted of end-stage kidney diseases (ESKD) and a reduction of estimated glomerular filtration rate (eGFR) by 50% or more. Logistic and Cox regression assessed the associations of hypertension subtypes with LVH and kidney outcomes.

Results: The 675 patients (425 [63.0%] men; mean [SD] age, 50.8 [15.9] years; mean [SD] eGFR, 61.6 [29.4] mL/min/1.73 m2) included 125 (19.3%) with controlled hypertension, 244 (37.6%) with MUCH, and 280 (43.1%) sustained hypertension. Among patients with MUCH, 2 (0.8%) had isolated daytime MUCH, 154 (63.1%) had isolated nighttime MUCH, and 88 (36.1%) had day-night MUCH. During a median (IQR) follow-up of 39 (19-64) months, 130 composite kidney events, including 97 ESKD events, occurred. Compared with controlled hypertension, MUCH and sustained hypertension were associated with LVH (eg, MUCH: odds ratio [OR], 2.94; 95% CI, 1.18-7.34; P = .02) and the composite kidney outcome (eg, MUCH: hazard ratio [HR], 4.12; 95% CI, 1.75-9.73; P = .001) after adjustment for age, sex, proteinuria, eGFR, and other baseline risk factors. Multivariate-adjusted associations were also significant between day-night MUCH and LVH (OR, 3.26; 95% CI, 1.15-9.25) and between isolated nighttime MUCH and the composite kidney outcome (HR, 4.27; 95% CI, 1.69-10.77).

Conclusions And Relevance: In this cohort study, nighttime MUCH was common and associated with LVH and poor kidney outcomes among patients with hypertension and nondialysis CKD. These findings suggest that ambulatory blood pressure monitoring was inadequately used in patients with CKD and hypertension, calling for more widespread use, even in patients with controlled office hypertension.
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http://dx.doi.org/10.1001/jamanetworkopen.2022.14460DOI Listing
May 2022

Measuring Corneal Astigmatism Using Optical Coherence Tomography in Keratoconus.

J Cataract Refract Surg 2022 May 26. Epub 2022 May 26.

Casey Eye Institute, Oregon Health & Science University, Portland, OR, USA.

Purpose: To measure net corneal astigmatism using optical coherence tomography (OCT, Avanti, Optovue) in individuals with keratoconus and compare its repeatability and accuracy with those obtained using Scheimpflug imaging (Pentacam HR, Oculus).

Setting: Casey Eye Institute, Portland, Oregon, USA.

Design: Prospective cross-sectional observational study.

Methods: Net corneal astigmatism was calculated in keratoconic and normal eyes with OCT and Scheimpflug imaging using four settings - pupil or vertex centration settings with a 3 or 4 mm circular analytical zone. Corneal elevation maps were obtained from OCT images and fitted with Zernike polynomials to obtain net corneal astigmatism. Manifest refraction astigmatism was used to evaluate the accuracy of net corneal astigmatism measurements. The coefficient of repeatability from two repeated measures was calculated.

Results: 46 eyes with manifest or subclinical keratoconus and 52 normal control eyes were analyzed. For OCT measurements in keratoconus, better accuracy was achieved with pupil centration and 3 mm analytical zone; however, better repeatability was achieved with vertex centration and 4 mm analytical zone (coefficient of repeatability = 0.53 D, Fligner-Killeen test with Bonferroni correction P < .0017). Agreement with manifest refraction was significantly better with OCT compared to Pentacam HR (generalized mixed-effect model with Bonferroni correction P < .00625). No significant difference was found between instruments or settings in control eyes.

Conclusions: OCT was able to measure net corneal astigmatism with better accuracy and precision than the Pentacam HR in keratoconic eyes. Measurements may be more accurate using pupil centration and a smaller analytical zone in subjects with keratoconus.
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http://dx.doi.org/10.1097/j.jcrs.0000000000000977DOI Listing
May 2022

It's all relative: Regression analysis with compositional predictors.

Biometrics 2022 May 26. Epub 2022 May 26.

Department of Statistics, University of Connecticut.

Compositional data reside in a simplex and measure fractions or proportions of parts to a whole. Most existing regression methods for such data rely on log-ratio transformations that are inadequate or inappropriate in modeling high dimensional data with excessive zeros and hierarchical structures. Moreover, such models usually lack a straightforward interpretation due to the interrelation between parts of a composition. We develop a novel relative-shift regression framework that directly uses proportions as predictors. The new framework provides a paradigm shift for regression analysis with compositional predictors and offers a superior interpretation of how shifting concentration between parts affects the response. New equi-sparsity and tree-guided regularization methods and an efficient smoothing proximal gradient algorithm are developed to facilitate feature aggregation and dimension reduction in regression. A unified finite-sample prediction error bound is derived for the proposed regularized estimators. We demonstrate the efficacy of the proposed methods in extensive simulation studies and a real gut microbiome study. Guided by the taxonomy of the microbiome data, the framework identifies important taxa at different taxonomic levels associated with the neurodevelopment of preterm infants. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/biom.13703DOI Listing
May 2022

Rapid Detection of MCR-Mediated Colistin Resistance in Escherichia coli.

Microbiol Spectr 2022 May 26:e0092022. Epub 2022 May 26.

College of Veterinary Medicine, Yangzhou Universitygrid.268415.c, Yangzhou, China.

Colistin is one of the last-resort antibiotics for infections caused by multidrug-resistant Gram-negative bacteria. However, the wide spread of novel plasmid-carrying colistin resistance genes and its variants substantially compromise colistin's therapeutic effectiveness and pose a severe danger to public health. To detect colistin-resistant microorganisms induced by genes, rapid and reliable antibiotic susceptibility testing (AST) is imminently needed. In this study, we identified an RNA-based AST (RBAST) to discriminate between colistin-susceptible and -mediated colistin-resistant bacteria. After short-time colistin treatment, RBAST can detect differentially expressed RNA biomarkers in bacteria. Those candidate mRNA biomarkers were successfully verified within colistin exposure temporal shifts, concentration shifts, and other variants. Furthermore, a group of clinical strains were effectively distinguished by using the RBAST approach during the 3-h test duration with over 93% accuracy. Taken together, our findings imply that certain mRNA transcripts produced in response to colistin treatment might be useful indicators for the development of fast AST for -positive bacteria. The emergence and prevalence of and its variants in humans, animals, and the environment pose a global public health threat. There is a pressing urgency to develop rapid and accurate methods to identify MCR-positive colistin-resistant bacteria in the clinical samples, providing a basis for subsequent effective antibiotic treatment. Using the specific mRNA signatures, we develop an RNA-based antibiotic susceptibility testing (RBAST) for effectively distinguishing colistin-susceptible and -mediated colistin-resistant strains. Meanwhile, the detection efficiency of these RNA biomarkers was evidenced in other variants-carrying strains. By comparing with the traditional AST method, the RBAST method was verified to successfully characterize a set of clinical isolates during 3 h assay time with over 93% accuracy. Our study provides a feasible method for the rapid detection of colistin-resistant strains in clinical practice.
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http://dx.doi.org/10.1128/spectrum.00920-22DOI Listing
May 2022

30-Year Experience With 22 Cases of Malignant Transformation Arising From Ovarian Mature Cystic Teratoma: A Rare Disease.

Front Oncol 2022 9;12:842703. Epub 2022 May 9.

Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, National Clinical Research Center for Obstetric & Gynecologic Diseases, Beijing, China.

Objective: To investigate the clinical characteristics and survival outcomes of patients with malignant transformation arising from ovarian mature cystic teratoma (MT-MCT).

Methods: This retrospective study included patients with ovarian MCTs at Peking Union Medical College Hospital (PUMCH) during 1990.01-2020.12. When the pathologic histology was MT-MCT, detailed information was collected.

Results: Overall, 7229 ovarian MCT patients and 22 patients with MT-MCT were enrolled. The rate of malignant transformation of all ovarian MCTs was 0.30%. Most patients with MT-MCT were 51 (21-75) years old, and the tumor mass size was 10 (3-30) cm. The typical clinical symptoms were mainly abdominal pain and distension. The levels of tumor markers were elevated on preoperative examination. Early diagnosis could be made by ultrasonic examination, pelvic enhanced MRI and CT. Most patients underwent debulking surgery and adjuvant chemotherapy. The most common histological type to exhibit malignant transformation was squamous cell carcinoma (59.1%), followed by adenocarcinoma (13.6%), carcinoid (9.1%), and borderline tumor (18.2%). The 5-year RFS and OS rates were 54.5% and 81.8%, respectively. Patients with FIGO stage I had the best RFS (P=0.047) and OS (P=0.018), followed by those with FIGO stage II-IV.

Conclusion: MT-MCTs mainly occur in elderly females, are rare and have a poor prognosis. Advanced FIGO stage is a risk factor for survival. Although there is no standard treatment, cytoreductive debulking surgery and adjuvant chemotherapy could be considered. Perimenopausal and menopausal women with MCT should receive surgical treatment.
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http://dx.doi.org/10.3389/fonc.2022.842703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124836PMC
May 2022

Global Transcriptome Analysis Revealed the Molecular Regulation Mechanism of Pigment and Reactive Oxygen Species Metabolism During the Stigma Development of .

Front Plant Sci 2022 9;13:881394. Epub 2022 May 9.

State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China.

Hickory ( Sarg.) is a monoecious plant of the genus of the Juglandaceae family. Its nuts contain a number of nutritional compounds and are deeply loved by consumers. Interestingly, it was observed that the color of hickory stigma changed obviously from blooming to mature. However, the molecular mechanism underlying color formation during stigma development and the biological significance of this phenomenon was mostly unknown. In this work, pigment content, reactive oxygen species (ROS) removal capacity, and transcriptome analysis of developing stigma of hickory at 4 differential sampling time points (S1, S2, S3, and S4) were performed to reveal the dynamic changes of related pigment, antioxidant capacity, and its internal molecular regulatory mechanism. It was found that total chlorophyll content was decreased slightly from S1 to S4, while total carotenoids content was increased from S1 to S3 but decreased gradually from S3 to S4. Total anthocyanin content continued to increase during the four periods of stigma development, reaching the highest level at the S4. Similarly, the antioxidant capacity of stigma was also gradually improved from S1 to S4. Furthermore, transcriptome analysis of developing hickory stigma identified 31,027 genes. Time-series analysis of gene expressions showed that these genes were divided into 12 clusters. Cluster 5 was enriched with some genes responsible for porphyrin and chlorophyll metabolism, carotenoid metabolism, and photosynthesis. Meanwhile, cluster 10 was enriched with genes related to flavonoid metabolism, including anthocyanin involved in ROS scavenging, and its related genes were mainly distributed in cluster 12. Based on the selected threshold values, a total of 10432 differentially expressed genes were screened out and enriched in the chlorophyll, carotenoid, anthocyanin, and ROS metabolism. The expression trends of these genes provided plausible explanations for the dynamic change of color and ROS level of hickory stigma with development. qRT-PCR analyses were basically consistent with the results of RNA-seq. The gene co-regulatory networks of pigment and ROS metabolism were further constructed and (CCA0887S0030) and (CCA0573S0068) were predicted to be two core transcriptional regulators. These results provided in-depth evidence for revealing the molecular mechanism of color formation in hickory stigma and its biological significance.
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http://dx.doi.org/10.3389/fpls.2022.881394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125253PMC
May 2022

In Situ Phototriggered Disulfide-Cross-Link Nanoparticles for Drug Delivery.

ACS Macro Lett 2016 Mar 11;5(3):301-305. Epub 2016 Feb 11.

Key Laboratory for Advanced Materials, Institute of Fine Chemicals, East China University of Science and Technology, 130# Meilong Road, Shanghai 200237, China.

In situ forming redox responsive nanoparticles have been developed based on amphiphilic copolymers-phototriggered disulfide-cross-link macromolecules (PDCM). Upon 405 nm light irradiation, the macrocyclic thiol caged coumarin phototrigger in PDCM can release free thiols, and these free thiols subsequently realize in situ disulfide cross-link via reacting with a pyridyl disulfide group inside the PDCM assembled nanoparticles. The phototriggered disulfide-cross-link strategy can be conducted rapidly, conveniently, and cleanly without adding any cross-linkers or catalysts. Via changing irradiation condition, nanoparticles with different cross-link densities can be formed. These nanoparticles can encapsulate hydrophobic guest molecules with good stability and achieve redox-triggered release under GSH reduction. Intracellular experiments show that these nanoparticles can be used as promising drug carriers.
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http://dx.doi.org/10.1021/acsmacrolett.5b00870DOI Listing
March 2016

Biogenesis of a bacterial metabolosome for propanediol utilization.

Nat Commun 2022 May 25;13(1):2920. Epub 2022 May 25.

Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool, L69 7ZB, United Kingdom.

Bacterial metabolosomes are a family of protein organelles in bacteria. Elucidating how thousands of proteins self-assemble to form functional metabolosomes is essential for understanding their significance in cellular metabolism and pathogenesis. Here we investigate the de novo biogenesis of propanediol-utilization (Pdu) metabolosomes and characterize the roles of the key constituents in generation and intracellular positioning of functional metabolosomes. Our results demonstrate that the Pdu metabolosome undertakes both "Shell first" and "Cargo first" assembly pathways, unlike the β-carboxysome structural analog which only involves the "Cargo first" strategy. Shell and cargo assemblies occur independently at the cell poles. The internal cargo core is formed through the ordered assembly of multiple enzyme complexes, and exhibits liquid-like properties within the metabolosome architecture. Our findings provide mechanistic insight into the molecular principles driving bacterial metabolosome assembly and expand our understanding of liquid-like organelle biogenesis.
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http://dx.doi.org/10.1038/s41467-022-30608-wDOI Listing
May 2022

Erfolgreiche perkutane Embolisation einer arteriellen Blutung nach CT-gesteuerter paravertebraler Biopsie: Ein Case Report.

Rofo 2022 Jun 25;194(6):663-664. Epub 2022 May 25.

Institute for Diagnostic and Interventional Radiology and Neuroradiology, University Hospital Essen, Germany.

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http://dx.doi.org/10.1055/a-1779-9293DOI Listing
June 2022

COVID-19 vaccine hesitancy and resistance in an urban Chinese population of Hong Kong: a cross-sectional study.

Hum Vaccin Immunother 2022 May 25:2072144. Epub 2022 May 25.

Department of Psychology, The University of Hong Kong, Hong Kong SAR, China.

Vaccine hesitancy against COVID-19 is prevalent. This study aimed to identify the factors associated with COVID-19 vaccination compliance among adults in Hong Kong. An online survey was conducted during an early stage of community-based COVID-19 vaccination campaign in Hong Kong. The questionnaire consisted of vaccine status, sociodemographic information, risk perception of being infected by COVID-19, and exposure to confirmed COVID cases, as well as items on sleep and mental health. The association between these variables and vaccine hesitancy was analyzed. Among the 883 participants (67.5% females, 54.5% aged 18-39), 30.6% had low vaccine hesitancy, 27.4% had high vaccine hesitancy, and 27.5% had vaccine rejection. The likelihood of having high vaccine hesitancy was higher among young (adjusted odds ratio [] = 2.99; 95% confidence interval [CI]: 1.23-7.30) and middle-aged respondents ( = 2.99; 95% CI: 1.07-5.47) than among old respondents. Moreover, those who were married ( = 0.51; 95% CI: 0.29-0.88), had a full-time job ( = 0.55; 95% CI: 0.29-0.88), and had a greater confidence in the government ( = 0.68; 95% CI: 0.54-0.86) were less likely to exhibit vaccine hesitancy. Our findings showed that the prevalence of vaccine hesitancy and vaccine resistance were high. Policy makers need specific strategies to target those who may have a high risk of vaccine hesitancy and resistance.
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http://dx.doi.org/10.1080/21645515.2022.2072144DOI Listing
May 2022

Designed assembly of heterometallic zeolite-like framework materials from two different supertetrahedral metal clusters.

Chem Commun (Camb) 2022 May 25. Epub 2022 May 25.

Fujian Provincial Key Laboratory of Advanced Inorganic Oxygenated-Materials, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.

This work demonstrates a feasible strategy for the synthesis of new zeolite-like framework materials. With the strategy of using two different supertetrahedral clusters as secondary building units, two new heterometallic zeolite-like frameworks with isomeric structures but tunable topologies were first made. Besides, the porous nature and the proton conduction performance of the new materials were further studied.
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http://dx.doi.org/10.1039/d2cc01833kDOI Listing
May 2022

Hydroxysafflor yellow A triggered a fast-to-slow muscle fiber-type conversion regulating FoxO1 in myocytes.

Food Funct 2022 May 25. Epub 2022 May 25.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Hydroxysafflor yellow A (HSYA) is the main bioactive component of safflower and has been reported to have significant health-promoting abilities. However, the regulation of HSYA on different types of skeletal myofibers is largely unknown. Here, experiments found that the water extract of safflower could significantly increase MyHC I, MB and Tnni1 mRNA expression while downregulating MyHC IIb mRNA expression. Furthermore, HSYA triggered fast-to-slow fiber-type switching and increased gene expression related to mitochondrial biosynthesis both and . Autodock analyses proved that FoxO1 is a potential target of HSYA, and qRT-PCR and western blotting further showed that HSYA significantly promoted the activation of the FoxO1 signaling pathway. Additionally, the levels of PGC1α, downstream of FoxO1, also significantly increased after HSYA treatment. Together, our findings suggested that HSYA triggered a fast-to-slow myofiber-type shift through the FoxO1 signaling pathway.
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http://dx.doi.org/10.1039/d1fo03612bDOI Listing
May 2022

SND1 acts as a functional target of miR-330-5p involved in modulating the proliferation, apoptosis and invasion of colorectal cancer cells.

Biochem Biophys Res Commun 2022 May 14;615:116-122. Epub 2022 May 14.

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China. Electronic address:

MicroRNAs (miRNAs) play a crucial role in cancer progression due to their capability to modulate the expression of various target genes. However, given the heterogeneity of tumor cells, miRNAs have been confirmed to exert different regulatory effects. Here, bioinformatic analysis results indicated that expression of miR-330-5p is decreased in colorectal cancer (CRC) tissues and inversely correlated with SND1 expression. Notably, ectopic expression of miR-330-5p restrained tumor cell proliferation, migration, and enhance the sensitivity of CRC cells to 5-FU. Moreover, similar phenotypes were substantiated after inhibition of SND1 expression using RNA interference. Conversely, overexpression of SND1 facilitated the malignant phenotypes of CRC cells and restored miR-330-5p-mediated tumor-suppressive activities in CRC cells. Mechanistically, miR-330-5p directly binds to SND1-3'-untranslated region (3'-UTR), thus involving in inhibiting CRC cells proliferation and invasion and promoting apoptosis. Taken together, miR-330-5p may act as a tumor suppressor by targeting the expression of SND1, suggesting that the miR-330-5p/SND1 axis may be a meaningful regulator for CRC intervention.
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http://dx.doi.org/10.1016/j.bbrc.2022.05.045DOI Listing
May 2022

Alterations of the Gut Microbiome and Fecal Metabolome in Colorectal Cancer: Implication of Intestinal Metabolism for Tumorigenesis.

Front Physiol 2022 4;13:854545. Epub 2022 May 4.

Department of Gastroenterology, Clinical Medical College and The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.

The gut microbiota and its metabolites are important for host physiological homeostasis, while dysbiosis is related to diseases including the development of cancers such as colorectal cancer (CRC). In this study, we characterized the relationship of an altered gut microbiome with the fecal metabolome in CRC patients in comparison with volunteers having a normal colorectal mucous membrane (NC). The richness and composition of the microbiota in fecal samples of 30 CRC patients and 36 NC controls were analyzed through 16S rRNA gene sequencing, and the metabolome was determined by ultra-performance liquid chromatography coupled to tandem mass spectrometry. Spearman correlation analysis was to determine the correlation between the gut microbiome and fecal metabolome in CRC patients. There were significant alterations in the gut microbiome and fecal metabolome in CRC patients compared with NC controls. Bacteroidetes, Firmicutes, Actinobacteriota, and Proteobacteria dominated the gut microbial communities at the phylum level in both groups. Compared with NC controls, CRC patients had a lower frequency of and but a higher abundance of and . Regarding the fecal metabolome, twenty-nine metabolites were identified as having significantly changed, showing increased levels of adrenic acid, decanoic acid, arachidonic acid, and tryptophan but a reduction in various monosaccharides in the fecal samples of CRC patients. Moreover, increased abundance of was strongly associated with decreased levels of monosaccharides, while was positively associated with the production of monosaccharides in the fecal samples. These results highlight alterations of gut microbiota in association with certain metabolites in CRC progression, implying potential diagnostic and intervention potential for CRC.
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http://dx.doi.org/10.3389/fphys.2022.854545DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9116530PMC
May 2022

The Role of Qi-Stagnation Constitution and Emotion Regulation in the Association Between Childhood Maltreatment and Depression in Chinese College Students.

Front Psychiatry 2022 4;13:825198. Epub 2022 May 4.

School of Fundamental Medical Science, Guangzhou University of Chinese Medicine, Guangzhou, China.

Background: Childhood maltreatment is known as a significant risk factor for later depression. However, there remains a lack of understanding about the mechanisms through which childhood maltreatment confers risk for depression. This study explores how Qi-stagnation constitution (QSC) and emotion regulation affect the link between childhood maltreatment and depressive symptoms in Chinese college students.

Methods: We recruited 2,108 college students aged 18-25 years between November 2020 and December 2021. Participants were required to complete four self-report questionnaires, including the Childhood Trauma Questionnaire-Short Form (CTQ-SF), Qi-Stagnation Constitution (QSC) subscale of the simplified Chinese Medicine Constitution Questionnaire, Difficulties in Emotion Regulation Scale (DERS), and the Beck Depression Inventory-II (BDI-II). Moderated mediation analyses were conducted.

Results: There was a positive correlation between childhood maltreatment and QSC, while the QSC partially mediated the effect of childhood maltreatment on depressive scores in college students. In addition, emotion dysregulation moderated the association between QSC and depressive scores.

Conclusion: These results enhance understanding of key factors influencing the link between childhood maltreatment and depressive symptoms among college students by combining the theory of TCM constitution with psychological processes. The development of strategies to prevent biased Qi-stagnation constitution and emotion dysregulation may help to improve college students' mental health and strengthen the resilience of individuals to depression.
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http://dx.doi.org/10.3389/fpsyt.2022.825198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114459PMC
May 2022

Migration, Schizophrenia, and Crime: A Study From a Forensic Psychiatric Sample.

Front Psychiatry 2022 6;13:869978. Epub 2022 May 6.

Department of Forensic Psychiatry, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Sichuan, China.

Background: The association between mental health problems and crime in immigrants has attracted recent academic interest, with results suggesting that there were possible interactions between immigration, schizophrenia, and criminal behavior. However, very few studies have examined these interactions, especially in developing countries that have mass internal immigration. Therefore, this study sought to identify the associations between the sociodemographic, clinical, and criminal factors in migrants and non-migrants with schizophrenia who had been involved in criminal activities in China.

Methods: Forensic archives of suspects who had been referred for criminal responsibility assessments in the Sichuan West China Forensic Center from January 2015 to December 2019 were reviewed. The sociodemographic, and criminal activity information of the suspects were extracted, while the clinical and social function were measured by the Brief Psychiatric Rating Scale (BPRS) and Social Disability Screening Schedule (SDSS) based on the archives. A Chi-squared test, a T-test, a Mann-Whitney U test, and Multinomial logistic regression were employed for the statistical analysis.

Results: A total of 552 patients were reviewed and evaluated, 17.2% ( = 95) of which were migrants. The migrant patient group was younger than the non-migrant patient group. The BPRS and SDSS scores for the migrant patient group were lower than for the non-migrant patient group. The migrant patient group had more work experience and more had been employed at the time of the crime than the non-migrant patient group. The unemployed migrant patients were more likely to commit a property-related crime.

Conclusions: Compared to the non-migrant schizophrenia patient group, the migrant patient group had less severe psychiatric symptoms and less social function impairments. Employment was an important factor in preventing criminality in patients with schizophrenia, especially for migrant patients. Vocational rehabilitation focuses on developing appropriate employment that can significantly assist in schizophrenia patient recovery, which in turn could reduce their chances of committing crime. Besides, reducing other obstacles to stigma, housing and health insurance would also be beneficial to crime reduction.
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http://dx.doi.org/10.3389/fpsyt.2022.869978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120637PMC
May 2022

Activation of M-AChR and IP/Ca/PKC signaling pathways by pilocarpine increases glycine-induced currents in ventral horn neurons of the spinal cord.

Neurosci Lett 2022 May 19:136690. Epub 2022 May 19.

Neurobiology Laboratory, Wannan Medical College, Wuhu, Anhui, 241002, China. Electronic address:

Our study aimed to determine the effects of pilocarpine and the mechanisms involving muscarinic acetylcholine receptors (mAChRs) on glycine receptors (GlyRs) in neurons of the spinal cord ventral horn. An enzymatic digestion combined with acute mechanical separation was applied to isolate neurons from the spinal cord ventral horn. Patch-clamp recording was then used to investigate the outcomes of pilocarpine. Our results indicate that pilocarpine increased glycine currents in a concentration-dependent manner, which was blocked by the M-AChR selective antagonists 4-DAMP and J104129. Pilocarpine also enhanced the glycine currents in nominally Ca-free extracellular solution. Conversely, the enhancement of glycine currents by pilocarpine disappeared when intracellular Ca was chelated by BAPTA. Heparin and Xe-C, which are IP receptor antagonists, also totally abolished the pilocarpine effect. Furthermore, Bis-IV, a PKC inhibitor, eliminated the pilocarpine effect. Additionally, PMA, a PKC activator, mimicked the pilocarpine effect. These results indicate that pilocarpine may increase glycine currents by activating the M-AChRs and IP/Ca/PKC pathways.
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http://dx.doi.org/10.1016/j.neulet.2022.136690DOI Listing
May 2022

Quantification of immunosuppressants from one 3.2 mm dried blood spot by a novel cold-induced phase separation based LC-MS/MS method.

Anal Chim Acta 2022 Jun 1;1210:339889. Epub 2022 May 1.

Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China. Electronic address:

Dried blood spots (DBS) have been regarded as a promising alternative for therapeutic drug monitoring (TDM) of immunosuppressants (ISDs) for over fifteen years. Nonetheless, there are still three main issues impeding its preference: (i) the requirement of relatively large disc; (ii) the controversial and intricate desorption approaches; (iii) the lack of feasible extraction strategies. For improvement, this work described a new LC-MS/MS method realizing quantification of four ISDs from one piece of 3.2 mm DBS. During sample pretreatment, a modified approach (infiltrating the DBS in pure water before adding acetonitrile and zinc sulfate as protein-precipitators) was developed to completely dissociate the targets from filter paper. Afterward, effective enrichment and purification of the targets were achieved by using cold-induced phase separation technique. Benefiting from these novelties, the method exhibited satisfying throughput (15 min for sample preparation), applicability (consuming only one 3.2 mm disc), reliability (82.3-107.8% for accuracy and <14.3% for precision) and sensitivity (lower limit of quantification of 0.5, 7.6, 0.7 and 0.8 ng mL for tacrolimus, cyclosporine A, everolimus and sirolimus, respectively). Without hematocrit correction, the method showed favorable interchangeability to the certified whole blood method through analyzing 120 paired clinical samples. By taking ±20% of the mean as the limit of acceptance for cross validation, over 90% of the detection met the criterion. It can be expected the developed method is able to further promote the popularization of DBS-based TDM for ISDs in practice.
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http://dx.doi.org/10.1016/j.aca.2022.339889DOI Listing
June 2022

Microplastics in urban soils of Nanjing in eastern China: Occurrence, relationships, and sources.

Chemosphere 2022 May 17:134999. Epub 2022 May 17.

School of Geographic and Oceanographic Sciences, Nanjing University, Nanjing, 210046, China; Key Laboratory of Coastal Zone Exploitation and Protection, Ministry of Natural Resources, Nanjing, 210024, China. Electronic address:

Although microplastic (MP) pollution has been defined as a new global challenge by the United Nations Environment Programme, their abundance and composition has only been studied in-depth within farmland soil, while minimal attention has been placed on urban soil contamination. Accordingly, within the current study, MP abundance and composition is investigated within urban soil from green spaces in Nanjing, eastern China. The average MP abundance in soil was 461 ± 222 items/kg and primarily comprised fibers (39.1%) and fragments (37.7%). MPs <1000 μm in size accounted for 83.7% of the total content and white MPs were the most abundant (26.5%). The dominant polymers were polyethylene glycol terephthalate (32.0%) and polypropylene (20.5%). Moreover, relationship network analysis generated three distinct MP modules based on community similarity. Indeed, the degree of similarity increased by ∼26.8% per kilometer. Furthermore, application of a forward selective optimal multiple regression model identified clay, sand, longitude, and points of interest for recycling bins (RecyclePOI) as the primary spatial and soil environmental factors affecting MP abundance and composition. Additionally, five potential sources of MPs were identified based on the MP diversity integrated index fitting results, and point of interest density (MDII-POI) source analysis (R = 0.21-0.62; P < 0.05). In particular, the point of interest of express delivery points (ExpressPOI) were important sources of plastic emissions as they are widely distributed throughout urban and fringe areas. Collectively, the findings of this study provide novel insights regarding quantitative source appointment and regional ecological control of MPs in urban soil.
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http://dx.doi.org/10.1016/j.chemosphere.2022.134999DOI Listing
May 2022

Early-life infection of the airways with Streptococcus pneumoniae exacerbates HDM-induced asthma in a murine model.

Cell Immunol 2022 May 14;376:104536. Epub 2022 May 14.

Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. Electronic address:

Respiratory tract infection early in life plays a significant role in the pathogenesis of asthma. In the present study we examine, using a murine surrogate, the effects of early life respiratory infection with Streptococcus pneumoniae (SP) on adult asthma induced by sensitisation and exposure to house dust mite (HDM) allergen. Mice (one week old) were infected with SP, then 3 weeks later sensitised to HDM emulsified with Al (OH)3 intraperitoneally and challenged intranasally with same allergen for up to a further 5 weeks to establish the asthma surrogate. Outcome measures were quantified using the FlexiVent apparatus, histology and immunohistology, ELISA and flow cytometry. The murine surrogates of asthma infected with SP early in life exhibited significantly more severe disease compared with the controls of mice without SP infection, as shown by airways responsiveness, inflammatory cellular infiltration of the airways, expression of markers of airways remodelling, serum concentrations of HDM-specific IgE and the concentrations of Th2-type cytokines and the numbers of activated Th2 and ILC2 cells in the lung tissues. These data are compatible with the hypothesis that early-life infection of the airways with SP exacerbates, at least in some individuals, subsequent HDM-induced allergic airways inflammation and associated asthma in adulthood in this murine surrogate.
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http://dx.doi.org/10.1016/j.cellimm.2022.104536DOI Listing
May 2022

RNF216 Alleviates Radiation-Induced Apoptosis and DNA Damage Through Regulating Ubiquitination-Mediated Degradation of p53 in Glioblastoma.

Mol Neurobiol 2022 May 20. Epub 2022 May 20.

Department of Neurovascular Intervention, Cangzhou Central Hospital, Cangzhou, Hebei, China.

Glioblastoma (GBM) is the most common and lethal subtype of glioma, characterized by uncontrolled cancer cell proliferation, extensive infiltration, and therapeutic resistance. Ring finger protein 216 (RNF216) is a RING-type E3 ubiquitin ligase aberrantly expressed in multiple human cancers. Tumor protein 53 (p53) is a transcription factor that acts as a tumor suppressor. This study aimed to compare the RNF216 expression in GBM tissues and normal peritumoral tissues and to examine the effects of RNF216 overexpression/knockdown on tumorigenesis, radioresistance, and the p53 pathway in GBM. The results showed that RNF216 was overexpressed in GBM tissues and cell lines, and high RNF216 expression was related to a poor prognosis. RNF216 overexpression promoted GBM cell growth and inhibited apoptosis, while RNF216 knockdown impaired GBM cell growth and enhanced cell death. RNF216 was also highly expressed in recurrent GBM tissues compared with paired primary tumors. The upregulation of RNF216 not only facilitated GBM cell growth but also protected cells against X-ray irradiation-induced apoptosis and DNA damage, while RNF216 knockdown exerted opposite effects. Moreover, the implantation of GBM cells with RNF216 silencing suppressed tumorigenesis and increased radiosensitivity of mice bearing GBM xenografts. Further analysis revealed that RNF216 overexpression reduced the stability of p53 protein via ubiquitination and negatively regulated the p53 pathway, while RNF216 knockdown preserved the p53 protein. In conclusion, RNF216 effectively attenuated radiation-induced apoptosis and DNA damage in GBM via inducing ubiquitination-mediated degradation of p53. These findings suggest the potential therapeutic use of RNF216 inhibition for tumorigenesis and therapeutic resistance in GBM.
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http://dx.doi.org/10.1007/s12035-022-02868-6DOI Listing
May 2022

Level of LncRNA GAS5 and Hippocampal Volume are Associated with the Progression of Alzheimer's Disease.

Clin Interv Aging 2022 9;17:745-753. Epub 2022 May 9.

Department of Neurology, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People's Republic of China.

Purpose: We evaluated the diagnostic value of long non-coding RNA growth arrest-specific transcript 5 (GAS5) and its relationship with hippocampal volume in Alzheimer's disease (AD).

Patients And Methods: One hundred and eight patients with AD and 83 healthy controls were included, and demographic data, biochemical parameters, GAS5 levels, and hippocampal volume were recorded. Chi-squared tests or independent sample t-tests were used to compare the baseline characteristics, relative expression of GAS5, and hippocampal volume. Correlations between variables were determined using Spearman's rank correlation test. Receiver operating characteristic (ROC) curves were generated to compare the diagnostic value of GAS5 and total hippocampal volume in AD.

Results: The levels of GAS5 were significantly upregulated in patients with AD compared with those in controls and were negatively correlated with MMSE score. There were differences in left hippocampal volume, right hippocampal volume, and total hippocampal volume between the two groups. Total hippocampal volume was positively correlated with MMSE score and negatively correlated with GAS5 expression in patients with AD. The area under the curve (AUC) of for GAS5 expression was 0.831, the sensitivity was 61.1%, and the specificity was 95.2%. The AUC of the combined total hippocampal volume was 0.891, the sensitivity was 74.1%, and the specificity was 92.8%.

Conclusion: The results suggested that GAS5 may be an excellent indicator of AD progression alone or in combination with hippocampal volume.
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http://dx.doi.org/10.2147/CIA.S363116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112342PMC
May 2022

Preventive Effect of Collagen Peptides from on Acute Kidney Injury through Attenuation of Oxidative Stress and Inflammation.

Oxid Med Cell Longev 2022 10;2022:8186838. Epub 2022 May 10.

Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.

The protective effect of collagen peptide from (Amp) on acute kidney injury (AKI) in mice and its mechanism were explored. The results showed that Amp-fed could effectively improve the renal mass index and histopathological morphology. The levels of serum creatinine and urea nitrogen decreased significantly, while the antioxidant enzyme catalase (CAT) and superoxide dismutase (SOD) increased significantly in Amp-fed groups. Western blot results disclosed that Amp significantly upregulates the levels of heme oxygenase-1 (HO-1), Nrf2, p-PI3K, and p-AKT in the kidney. In addition, Amp could significantly downregulate the levels of nuclear factor NF-kappa-B (NF-B), tumor necrosis factor (TNF-), inflammatory cytokines interleukin 6 (IL-6) and interleukin 1 (IL-1). These findings provide evidence that Amp plays a protective role in AKI via attenuation of oxidative stress and inflammation mediated by PI3K/AKT/Nrf2 and PI3K/AKT/NF-B pathways. This study laid a foundation for the application of Amp in the prevention of AKI.
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http://dx.doi.org/10.1155/2022/8186838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113864PMC
May 2022

Synergistic antibacterial photocatalytic and photothermal properties over bowl-shaped TiO nanostructures on Ti-19Zr-10Nb-1Fe alloy.

Regen Biomater 2022 4;9:rbac025. Epub 2022 May 4.

School of Materials Science and Engineering, Beihang University, Beijing 100191, China.

As implant substitutes are increasingly applied to the clinic, the infection caused by implants has become one of the most common complications, and the modification of the antibacterial function of the implant can reduce such complications. In this work, a well-defined bowl-shaped nanostructure coating with photocatalytic and photothermal synergistic antibacterial properties was prepared on Ti-19Zr-10Nb-1Fe (TZNF) alloy. The coating is obtained by spin-coating and sintering TiO precursors templated from self-assembled microspheres of polystyrene-poly(4-vinylpyridine) (PS-P4VP) amphiphilic block polymer on TZNF alloy. PS-P4VP provides the bowl-shaped TiO nanostructures doped with C, N elements, reducing the band-gap of TiO, which can absorb near-infrared (NIR) light to release reactive oxygen species and produce photothermal conversion. The bowl structure is expected to enhance the utilization of light via the reflection in the confined space. The bowl-shaped surface has 100% antibacterial rates after 30 min of NIR light irradiation. In addition to antibacterial properties, the bowl-shaped surface has better hydrophilicity and protein adsorption capacity. The amount of protein adsorbed on TZNF with the bowl-shaped structures was six times that of TZNF. Hence, the bowl-shaped nanostructure can promote the proliferation and adhesion of osteoblasts, the cell proliferation rate was increased by 10-30%.
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http://dx.doi.org/10.1093/rb/rbac025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113230PMC
May 2022

Waterborne Polyurethane Acrylates Preparation towards 3D Printing for Sewage Treatment.

Materials (Basel) 2022 May 5;15(9). Epub 2022 May 5.

Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.

Conventional immobilized nitrifying bacteria technologies are limited to fixed beds with regular shapes such as spheres and cubes. To achieve a higher mass transfer capacity, a complex-structured cultivate bed with larger specific surface areas is usually expected. Direct ink writing (DIW) 3D printing technology is capable of preparing fixed beds where nitrifying bacteria are embedded in without geometry limitations. Nevertheless, conventional bacterial carrier materials for sewage treatment tend to easily collapse during printing procedures. Here, we developed a novel biocompatible waterborne polyurethane acrylate (WPUA) with favorable mechanical properties synthesized by introducing amino acids. End-capped by hydroxyethyl acrylate and mixed with sodium alginate (SA), a dual stimuli-responsive ink for DIW 3D printers was prepared. A robust and insoluble crosslinking network was formed by UV-curing and ion-exchange curing. This dual-cured network with a higher crosslinking density provides better recyclability and protection for cryogenic preservation. The corresponding results show that the nitrification efficiency for printed bioreactors reached 99.9% in 72 h, which is faster than unprinted samples and unmodified WPUA samples. This work provides an innovative immobilization method for 3D printing bacterial active structures and has high potential for future sewage treatment.
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http://dx.doi.org/10.3390/ma15093319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104063PMC
May 2022

Ginkgolic acid and anacardic acid are reversible inhibitors of SARS-CoV-2 3-chymotrypsin-like protease.

Cell Biosci 2022 May 19;12(1):65. Epub 2022 May 19.

Institute for Drug Screening and Evaluation, Wannan Medical College, Wuhu, 241002, China.

Because of the emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in different regions of the world, the battle with infectious coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has been seesawing. Therefore, the identification of antiviral drugs is of particular importance. In order to rapidly identify inhibitors for SARS-CoV-2 3-chymotrypsin-like protease (3CL), an enzyme essential for viral replication, we combined the fluorescence polarization (FP) technique with biotin-avidin system (BAS) and developed a novel sandwich-like FP screening assay. Through high-throughput screening, two hits of 3CL inhibitors, ginkgolic acid (GA) and anacardic acid (AA) were identified, which showed IC values of 11.29 ± 0.48 and 12.19 ± 0.50 μM, respectively. Their binding modes were evaluated by HPLC-Q-TOF-MS. There was no mass increase detected for SARS-CoV-2 3CL incubated with either GA or AA, indicating the absence of covalent adducts. The kinetic analysis clearly demonstrated that both GA and AA inhibit SARS-CoV-2 3CL via reversible and mixed-inhibition manner. Our results argue against conclusion that GA and AA act as irreversible and covalent inhibitors against SARS-CoV-2 3CL, which is based on the studies by Chen et al.
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http://dx.doi.org/10.1186/s13578-022-00806-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118179PMC
May 2022

Encapsulating N‑doped graphite carbon in MoO as a novel cocatalyst for boosting photocatalytic hydrogen evolution.

J Colloid Interface Sci 2022 May 11;623:267-276. Epub 2022 May 11.

Hebei Key Laboratory of Photoelectric Control on Surface and Interface, College of Science, Hebei University of Science and Technology, Shijiazhuang 050018, China. Electronic address:

Generally, it is important to ameliorate the co-catalyst used in photocatalytic hydrogen evolution reactions (PHERs) to achieve efficient transfer and separation of photogenerated carriers, decrease the surface reaction energy barrier, and hence improve the photocatalytic activity. In this study, N-doped graphite carbon (GC) was introduced in situ to MoO to ensure the presence of well-dispersed active sites, lower the overpotential of hydrogen evolution, and further achieve high conductivity. Then, the MoO/GC composite obtained was used as a co-catalyst of ZnInS (ZIS) in a PHER, resulting in a great improvement in the photocatalytic activity. Given the metallicity and large work function of MoO/GC, a Schottky interface can form between MoO/GC and ZIS, which accelerates the transmission of photogenerated electrons. As a result, the separation efficiency of photogenerated carriers improves, whereas the surface overpotential of PHERs clearly decreases for ZIS. This study proposes a new idea for exploiting efficient co-catalysts and promotes the wide and heavy use of carbon materials in the field of solar energy conversion.
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http://dx.doi.org/10.1016/j.jcis.2022.05.046DOI Listing
May 2022

Diagnostic Fragmentation Pathways for Identification of Phthalate Metabolites in Nontargeted Analysis Studies.

J Am Soc Mass Spectrom 2022 May 19. Epub 2022 May 19.

Exposure and Biomonitoring Division, Environmental Health Science and Research Bureau, Environmental and Radiation Health Sciences Directorate, Healthy Environments and Consumer Safety Branch, Health Canada, AL: 2203 B, 251 Sir Frederick Banting Driveway, Ottawa, Ontario K1A 0K9, Canada.

Phthalates have been studied due to their linkages with adverse developmental effects; however, metabolites of this class of compounds are undercharacterized and are poorly captured by traditional targeted analysis. In this study, we developed a nontargeted analysis approach for identifying and classifying phthalate metabolites based on a comprehensive study of their fragmentation pathways in electrospray ionization (ESI) quadrupole-time-of-flight mass spectrometry (QTOF-MS). This approach identifies molecular features in the data as phthalate metabolites via the detection of three structurally significant fragment ions. Then phthalate metabolites are classified into four types based on the presence of additional fragment ions specific to each type. Cleavage mechanisms for each class of phthalate metabolite are proposed based on fragmentation patterns generated at various collision energies (CE). All of the tested phthalate metabolites including oxidative and nonoxidative metabolites produced a fragment ion at / 121.0295, representing the deprotonated benzoate ion [CHCOO]. Most tested phthalate metabolites can produce a specific ion at / 147.0088, the deprotonated -phthalic anhydride ion. However, phthalate carboxylate metabolites can only produce the [M-H-R] ion at / 165.0193 and do not produce the fragment at / 147.0088. Other phthalate oxidative metabolites (hydroxyl- and oxo-) follow a different fragmentation pathway than nonoxidative metabolites. With this workflow, eight unknown phthalate metabolites were putatively identified in pooled urine, with one identified as a previously unreported metabolite by a combination of the MS/MS spectrum and the predicted retention time. Method detection limits for phthalate metabolites in urine were also estimated.
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http://dx.doi.org/10.1021/jasms.2c00052DOI Listing
May 2022

ddPCR surpasses classical qPCR technology in quantitating bacteria and fungi in the environment.

Mol Ecol Resour 2022 May 19. Epub 2022 May 19.

CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.

Quantitative real-time PCR (qPCR) has been widely used in quantifying bacterial and fungal populations in various ecosystems, as well as the fungi to bacteria ratio (F:B ratio). Recently, researchers have begun to apply droplet digital PCR (ddPCR) to this area, however, no study has systematically compared qPCR and ddPCR for quantitating both bacteria and fungi in environmental samples at the same time. Here, we designed probe-primer pair combinations targeting the 16S rRNA gene and Internal Transcribed Spacer (ITS) for the detection of bacteria and fungi respectively, and tested both SYBR Green and TaqMan approaches in qPCR and ddPCR methods for mock communities and in real environmental samples. In mock communities, the quantification results of ddPCR were significantly closer to expected values (P < 0.05), and had smaller coefficients of variations (P < 0.05) than qPCR, suggesting ddPCR was more accurate and repeatable. In environmental samples, ddPCR consistently quantified ITS and 16S rRNA gene concentrations in all four habitats without abnormal overestimation or underestimation, and the F:B ratio obtained by ddPCR was consistent with phospholipid fatty acid analysis. Our results indicated that ddPCR had better precision, repeatability, sensitivity, and stability in bacterial and fungal quantitation than qPCR. Although ddPCR has high cost, complicated processes and restricted detection range, it shows insensitivity to PCR inhibitors and the potential of quantifying long target fragments. We expect that ddPCR, which is complementary to qPCR, will contribute to microbial quantification in environmental monitoring and evaluation.
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http://dx.doi.org/10.1111/1755-0998.13644DOI Listing
May 2022
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