Publications by authors named "Yan Jiao"

279 Publications

Discovery of 3,6-disubstutited-imidazo[1,2-a]pyridine derivatives as a new class of CLK1 inhibitors.

Bioorg Med Chem Lett 2021 Mar 1:127881. Epub 2021 Mar 1.

Key Laboratory of Drug Targeting and Drug Delivery System of Ministry of Education, West China School of Pharmacy, Sichuan University, Sichuan 610041, China. Electronic address:

Inhibition of cdc2-like kinase1 (CLK1) could efficiently induce autophagy and it has been thought as a potential target for treatment of autophagy-related diseases. Herein we report the discovery of a series of 3,6-disubstutited-imidazo[1,2-a]pyridine derivatives as a new class of CLK1 inhibitors. Among them, compound 9e is the most potent one, which exhibits an IC value of 4 nM against CLK1 kinase. In vitro, this compound reduces the phosphorylation level of the typical downstream substrates of CLK1 and affects their subcellular redistribution. Further study indicates that 9e is efficient to induce autophagy. Overall, this study provides a promising lead compound for drug discovery targeting CLK1 kinase.
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http://dx.doi.org/10.1016/j.bmcl.2021.127881DOI Listing
March 2021

[Corrigendum] Rab22a is a novel prognostic marker for cell progression in breast cancer.

Int J Mol Med 2021 Mar 11;47(3). Epub 2021 Jan 11.

Department of Breast Surgery, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.

Following the publication of this article, the authors have realized that Table I contained an error: The number of patients who were alive in the Rab22a high expression group should have been written as 77 instead of 772.
A corrected version of the Table is shown on the next page (the corrected datum is highlighted in bold). The authors sincerely apologize for the error that was introduced during the preparation of this Table, and regret any inconvenience that this mistake has caused. [the original article was published in International Journal of Molecular Medicine 45: 1037-1046, 2020; DOI: 10.3892/ijmm.2020.4486].
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http://dx.doi.org/10.3892/ijmm.2021.4851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834961PMC
March 2021

Downregulated mRNA Expression of ZNF385B Is an Independent Predictor of Breast Cancer.

Int J Genomics 2021 3;2021:4301802. Epub 2021 Feb 3.

Department of Anesthesia, The Second Hospital of Jilin University, Changchun, Jilin 130022, China.

Background: ZNF385B, a zinc finger protein, has been known as a potential biomarker in some neurological and hematological studies recently. Although numerous studies have demonstrated the potential function of zinc finger proteins in tumor progression, the effects of ZNF385B in breast cancer (BC) are less studied.

Methods: The Oncomine database and "ESurv" tool were used to explore the differential expression of ZNF385B in pan-cancer. Furthermore, data of patients with BC were downloaded from The Cancer Genome Atlas (TCGA). The receiver operating characteristic (ROC) curve of ZNF385B expression was established to explore the diagnostic value of ZNF385B and to obtain the cut-off value of high or low ZNF385B expression in BC. The chi-square test as well as Fisher exact test was used for identification of the relationships between clinical features and ZNF385B expression. Furthermore, the effects of ZNF385B on BC patients' survival were evaluated by the Kaplan-Meier and Cox regression. Data from the Gene Expression Omnibus (GEO) database were employed to validate the results of TCGA. Protein expression of ZNF385B in BC patient specimens was detected by immunohistochemistry (IHC) staining.

Results: ZNF385B expression was downregulated in most types of cancer including BC. Low ZNF385B expression was related with survival status, overall survival (OS), and recurrence of BC. ZNF385B had modest diagnostic value, which is indicated by the area under the ROC curve (AUC = 0.671). Patients with lower ZNF385B expression had shorter OS and RFS (relapse-free survival). It had been demonstrated that low ZNF385B expression represented independent prognostic value for OS and RFS by multivariate survival analysis. The similar results were verified by datasets from the GEO database as well. The protein expression of ZNF385B was decreased in patients' samples compared with adjacent tissues by IHC.

Conclusions: Low ZNF385B expression was an independent predictor for worse prognosis of BC patients.
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http://dx.doi.org/10.1155/2021/4301802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876827PMC
February 2021

Hydrogeochemical characterization of a possible carbon sink from shallow saline-alkaline groundwater in the eastern Hetao Basin of Inner Mongolia in China.

Environ Sci Process Impacts 2021 Mar;23(2):344-356

Chemistry and Environmental Science College, Inner Mongolia Normal University, Hohhot 010022, China. and Inner Mongolia Key Laboratory of Environmental Chemistry, Hohhot 010022, China.

The question of how saline-alkaline groundwater can be used as a CO2 sink in arid saline-alkaline areas remains controversial. This study investigates the role of saline-alkaline groundwater as a CO2 sink using a mass balance method, Gibbs diagrams of the hydrochemistry, and carbon isotope (δ13CDIC) measurements. Twenty-eight groundwater samples of varying electrical conductivity (EC; 1.52-52.34 mS cm-1) were collected at different depths (1-2 and 5-25 m) in the Hetao Basin of Inner Mongolia, China. The results showed that groundwater ions could be primarily concentrated from water-rock interactions and evaporation, and that there are two main groundwater types: a mixed Na·Ca·Mg-Cl·SO4·HCO3 type and a Na-Cl type. The dissolved inorganic carbon (DIC) concentration in samples obtained from a depth of 1-2 m was less than that in samples from 5-25 m, and a downward migration trend of DIC in the groundwater was observed. The DIC concentration exhibited a significant positive correlation with pH (R2 = 0.61, p < 0.05) and the saturation index of carbonates (R2 = 0.93, p < 0.01). Groundwater with a higher pH contained a higher DIC concentration and could provide strong carbon sink potential. The δ13CDIC values of the groundwater samples varied from -21.22‰ to -11.02‰, indicating that DIC was derived from the dissolution equilibrium of pedogenic carbonates and atmospheric/soil CO2. The carbon sequestration of the shallow saline-alkaline groundwater in the Hetao Basin could reach 4.66 × 108 g C a-1, which represents important potential of carbon sink in the biogeochemical cycle.
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http://dx.doi.org/10.1039/d0em00415dDOI Listing
March 2021

PGC-1α protects from myocardial ischaemia-reperfusion injury by regulating mitonuclear communication.

J Cell Mol Med 2021 Jan 19. Epub 2021 Jan 19.

Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun, China.

The recovery of blood supply after a period of myocardial ischaemia does not restore the heart function and instead results in a serious dysfunction called myocardial ischaemia-reperfusion injury (IRI), which involves several complex pathophysiological processes. Mitochondria have a wide range of functions in maintaining the cellular energy supply, cell signalling and programmed cell death. When mitochondrial function is insufficient or disordered, it may have adverse effects on myocardial ischaemia-reperfusion and therefore mitochondrial dysfunction caused by oxidative stress a core molecular mechanism of IRI. Peroxisome proliferator-activated receptor gamma co-activator 1α (PGC-1α) is an important antioxidant molecule found in mitochondria. However, its role in IRI has not yet been systematically summarized. In this review, we speculate the role of PGC-1α as a key regulator of mitonuclear communication, which may interacts with nuclear factor, erythroid 2 like -1 and -2 (NRF-1/2) to inhibit mitochondrial oxidative stress, promote the clearance of damaged mitochondria, enhance mitochondrial biogenesis, and reduce the burden of IRI.
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http://dx.doi.org/10.1111/jcmm.16236DOI Listing
January 2021

Thylakoid Membranes with Unique Photosystems Used to Simultaneously Produce Self-Supplying Oxygen and Singlet Oxygen for Hypoxic Tumor Therapy.

Adv Healthc Mater 2021 Jan 14:e2001666. Epub 2021 Jan 14.

Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.

Photodynamic therapy (PDT) efficacy has been dramatically limited by the insufficient oxygen (O ) level in hypoxic tumors. Although various PDT nanosystems have been designed to deliver or produce O in support of reactive oxygen species (ROS) formation, the feature of asynchronous O generation and ROS formation still results in the low PDT efficacy. Herein, thylakoid membranes (TM) of chloroplasts is decorated on upconversion nanoparticles (UCNPs) to form UCTM NPs, aiming at realizing spatiotemporally synchronous O self-supply and ROS production. Upon 980 nm laser irradiation, UC NPs can emit the red light to activate both photosystem-I and photosystem-II of TM, the Z-scheme electronic structure of which facilitates water to produce O and further to singlet oxygen ( O ). UCTM NPs showed excellent biocompatibility, and can effectively remove the hypoxic tumor of mice upon 980 nm laser irradiation. This study develops a new PDT strategy for hypoxic tumor therapy based on photosynthesis.
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http://dx.doi.org/10.1002/adhm.202001666DOI Listing
January 2021

Association between population density and infection rate suggests the importance of social distancing and travel restriction in reducing the COVID-19 pandemic.

Environ Sci Pollut Res Int 2021 Jan 13. Epub 2021 Jan 13.

Center of Integrative Research, The First Hospital of Qiqihar City, 30 Gongyuan Road, Qiqihar, Heilongjiang, 161005, People's Republic of China.

Currently, 2019-nCoV has spread to most countries of the world. Understanding the environmental factors that affect the spread of the disease COVID-19 infection is critical to stop the spread of the disease. The purpose of this study is to investigate whether population density is associated with the infection rate of the COVID-19. We collected data from official webpages of cities in China and in the USA. The data were organized on Excel spreadsheets for statistical analyses. We calculated the morbidity and population density of cities and regions in these two countries. We then examined the relationship between morbidity and other factors. Our analysis indicated that the population density in cities in Hubei province where the COVID-19 was severe was associated with a higher percentage of morbidity, with an r value of 0.62. Similarly, in the USA, the density of 51 states and territories is also associated with morbidity from COVID-19 with an r value of 0.55. In contrast, as a control group, there is no association between the morbidity and population density in 33 other regions of China, where the COVID-19 epidemic is well under control. Interestingly, our study also indicated that these associations were not influenced by the first case of COVID-19. The rate of morbidity and the number of days from the first case in the USA have no association, with an r value of - 0.1288. Population density is positively associated with the percentage of patients with COVID-19 infection in the population. Our data support the importance of such as social distancing and travel restriction in the prevention of COVID-19 spread.
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http://dx.doi.org/10.1007/s11356-021-12364-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806252PMC
January 2021

Adenylate kinase 7 is a prognostic indicator of overall survival in ovarian cancer.

Medicine (Baltimore) 2021 Jan;100(1):e24134

From Reproductive Medical Center, Department of Obstetrics and Gynecology, The Second Hospital of Jilin University.

Abstract: Ovarian cancer (OC), a common malignant heterogeneous gynecological tumor, is the primary cause of cancer-related death in women worldwide. Adenylate kinase (AK) 7 belongs to the adenylate kinase (AK) family and is a cytosolic isoform of AK. Recent studies have demonstrated that AK7 is expressed in several human diseases, including cancer. However, there is a scarcity of reports on the relationship between AK7 and OC. Here, we compared the expression of AK7 in normal and cancerous ovarian tissues from The Cancer Genome Atlas database and used the c2 test to assess the correlation between AK7 levels and the clinical symptoms of OC. Finally, the prognostic significance of AK7 in OC was determined using the Kaplan-Meier analyses and Cox regression and performed gene set enrichment analysis to detect any relevant signaling pathways. We found that AK7 levels were substantially downregulated in OC than that in normal ovarian tissues (P < .001). Low AK7 levels were related to the patients' age (P = .0093) in OC. The median overall survival (OS) of patients with low AK7-expressing OC was shorter than patients with high AK7-expressing OC (P = .019). The Cox regression analysis (multivariate) identified low AK7 levels were independently related to the prognosis of OC (HR 1.34; P = .048). Our study demonstrated that the downregulated levels of AK7 could serve as an independent prognostic indicator for the OS in OC. Additionally, gene set enrichment analysis revealed that EMT, apical junction, TGF-b signaling, UV response, and myogenesis were associated in the low AK7 expression phenotype (NOM P < .05).
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http://dx.doi.org/10.1097/MD.0000000000024134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793326PMC
January 2021

MicroRNA-520c-3p suppresses vascular endothelium dysfunction by targeting RELA and regulating the AKT and NF-κB signaling pathways.

J Physiol Biochem 2021 Jan 7. Epub 2021 Jan 7.

Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China.

Endothelial injury, which can cause endothelial inflammation and dysfunction, is an important mechanism for the development of atherosclerotic plaque. This study aims to investigate the functional role of miR-520c-3p in vascular endothelium during inflammatory diseases such as atherosclerosis. Quantitative real-time PCR was used to detect miR-520c-3p expression in in human umbilical vein endothelial cells (HUVECs) after treatment with platelet-derived growth factor (PDGF). Furthermore, the effects of miR-520c-3p overexpression and silencing on cell proliferation, adhesion, and apoptosis were assessed. Bioinformatics analysis and Biotin-labeled miRNA pull-down assay were used to confirm the targets of miR-520-3p. Then, the effects of miR-520c-3p on AKT and NF-κB signaling pathways were detected by western blot. Herein, we observed that the expression level of miR-520c-3p was downregulated in HUVECs under PDGF stimulation. Overexpression of miR-520c-3p not only decreased cell adhesion but also promoted proliferation and inhibited apoptosis to protect the viability of endothelial cells. It was confirmed that RELA is the target of miR-520c-3p. MiR-520c-3p inhibited the protein phosphorylation of AKT and RELA, and si-RELA reversed the promotion of AKT and RELA protein phosphorylation by anti-miR-520c-3p. In summary, our study suggested that miRNA-520c-3p targeting RELA through AKT and NF-κB signaling pathways regulated the proliferation, apoptosis, and adhesion of vascular endothelial cells. We conclude that miR-520c-3p may play an important role in the suppression of endothelial injury, which could serve as a biomarker and therapeutic target for atherosclerosis.
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http://dx.doi.org/10.1007/s13105-020-00779-5DOI Listing
January 2021

A cost-effective plan for global testing - an infection rate stratified, algorithm guided, multiple-level, continuously pooled testing strategy.

Sci Total Environ 2021 Apr 24;765:144251. Epub 2020 Dec 24.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, PR China. Electronic address:

The most effective measure to prevent or stop the spread of infectious diseases is the early identification and isolation of infected individuals through comprehensive screening. At present, in the COVID-19 pandemic, such screening is often limited to isolated regions as determined by local governments. Screening of potentially infectious individuals should be conducted through coordinated national or global unified actions. Our current research focuses on using resources to conduct comprehensive national and regional regular testing with a risk rate based, algorithmic guided, multiple-level, pooled testing strategy. Here, combining methodologies with mathematical logistic models, we present an analytic procedure of an overall plan for coordinating state, national, or global testing. The proposed plan includes three parts 1) organization, resource allocation, and distribution; 2) screening based on different risk levels and business types; and 3) algorithm guided, multiple level, continuously screening the entire population in a region. This strategy will overcome the false positive and negative results in the polymerase chain reaction (PCR) test and missing samples during initial tests. Based on our proposed protocol, the population screening of 300,000,000 in the US can be done weekly with between 15,000,000 and 6,000,000 test kits. The strategy can be used for population screening for current COVID-19 and any future severe infectious disease when drugs or vaccines are not available.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833620PMC
April 2021

Wntless (Wls): A Prognostic Index for Progression and Patient Survival of Breast Cancer.

Onco Targets Ther 2020 8;13:12649-12659. Epub 2020 Dec 8.

Department of Breast Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People's Republic of China.

Background: Wntless (Wls) is an essential protein that is necessary for the secretion of Wnt proteins. While numerous researches have demonstrated that aberrations in Wnt/β-catenin expression lead to tumorigenesis and progression in many cancer types, the effects of Wls in breast cancer (BC) are less studied.

Methods: The mRNA and protein expression of Wls in BC cell lines were detected by RT-qPCR and Western blot; the protein expression of patient samples was detected by immunohistochemistry (IHC). The associations between Wls expression and clinicopathological factors as well as survival time, including overall survival (OS) and disease-free survival (DFS) were analyzed. Bioinformatics analysis was used to reveal the correlation between Wls genes and associated genes or pathways.

Results: Wls was overexpressed in BC cell lines and tissues. The expression level of Wls was significantly correlated with tumor size, estrogen receptor (ER), progesterone receptor (PR), Ki-67, molecular classification, and follow-up status. Spearman correlation analysis showed that Wls protein expression was negatively correlated with ER and PR, which was confirmed by bioinformatics analysis in mRNA level. However, there were positive relationships with MBNG (modified Black's nuclear grade), tumor size, Ki-67, molecular classification, follow-up, and vital status. Univariate and multivariate analysis showed that Wls was an independent prognostic factor for OS and DFS in BC patients. Moreover, Wls was a significant prognostic indicator of OS and DFS in a hormone receptor-positive (HR+) subgroup. GSEA showed that estrogen and androgen response, as well as epithelial-mesenchymal transition pathways, were up-regulated in the Wls high-expression group.

Conclusion: Overexpression of Wls is a significant marker of worse prognosis in BC and might play a crucial role in the HR+ subgroup.
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http://dx.doi.org/10.2147/OTT.S265324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7737487PMC
December 2020

Using exosomal miRNAs extracted from porcine follicular fluid to investigate their role in oocyte development.

BMC Vet Res 2020 Dec 14;16(1):485. Epub 2020 Dec 14.

College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Road Dingxing 7#, Loudi City, 417000, HuNan Province, China.

Background: Follicular development is crucial to normal oocyte maturation, with follicular size closely related to oocyte maturation. To better understand the molecular mechanisms behind porcine oocyte maturation, we obtained exosomal miRNA from porcine follicular fluid (PFF). These miRNA samples were then sequenced and analyzed regarding their different follicular sizes, as described in the methods section.

Results: First, these results showed that this process successfully isolated PFF exosomes. Nearly all valid reads from the PFF exosomal sequencing data were successfully mapped to the porcine genome database. Second, we used hierarchical clustering methods to determine that significantly expressed miRNAs were clustered into A, B, C, and D groups in our heatmap according to different follicle sizes. These results allowed for the targeting of potential mRNAs genes related to porcine oocyte development. Third, we chose ten, significantly expressed miRNAs and predicted their target genes for further GO analysis. These results showed that the expression levels of neurotransmitter secretion genes were greatly changed, as were many target genes involved in the regulation of FSH secretion. Notably, these are genes that are very closely related to oocyte maturation in growing follicles. We then used pathway analysis for these targeted genes based on the originally selected ten miRNAs. Results indicated that the pathways were mainly related to the biosynthesis of TGF-beta and its signaling pathway, which are very closely related to reproductive system functions.

Conclusions: Finally, these exosomal miRNAs obtained from PFF may provide a valuable addition to our understanding of the mechanism of porcine oocyte maturation. It is also likely that these exosomal miRNAs could function as molecular biomarkers to choose high-quality oocytes and allow for in vitro porcine embryo production.
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http://dx.doi.org/10.1186/s12917-020-02711-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7737261PMC
December 2020

Analysis of the lateral slope's impact on the calculation of water-filled rut depth.

PLoS One 2020 11;15(12):e0243952. Epub 2020 Dec 11.

School of Highway, Chang'an University, Xi'an, Shaanxi, China.

Accurate calculation of the water-filled rut depth is critical for assessing hydroplaning potential. Nevertheless, due to the difficulty in collecting and calculating the water-filled rut depth, most transportation agencies do not use i, especially in the case of lateral slopes, although water-filled rut depth is a key parameter that impacts driving safety. Contributions of this paper are development of a methodology to reliably compute the water-filled rut depth and quantitatively evaluate the influence of lateral slope on the water-filled rut depth. The proposed method include: 1) acquisition of the high-resolution 3D point cloud data of rut, 2) smooth processing of rut profile through moving average method with Matlab programming, 3) water-filled rut depth computation at different lateral slopes with the assistance of key points based on rut sections. With the variation of water-filled rut depth (ΔWD), its change rate (δWD), and the calculation error between the rut depth and the water-filled rut depth (Δn) as evaluation indexes, the variation law of water-filled rut depth under different lateral slopes is analyzed when considering the severity level and rut shape of the rut profile. Results show that: 1) the increase in lateral slope leads to the reduction of water-filled rut depth; 2) the water-filled rut depth is affected by the rut shape, including rut side wall's slope and the key points' elevation. The accurate calculation of the water-filled rut depth can provide reliable suggestions for safe driving.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0243952PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732103PMC
February 2021

Detection of fish movement patterns across management unit boundaries using age-structured Bayesian hierarchical models with tag-recovery data.

PLoS One 2020 7;15(12):e0243423. Epub 2020 Dec 7.

Department of Fish and Wildlife Conservation, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America.

Tagging studies have been widely conducted to investigate the movement pattern of wild fish populations. In this study, we present a set of length-based, age-structured Bayesian hierarchical models to explore variabilities and uncertainties in modeling tag-recovery data. These models fully incorporate uncertainties in age classifications of tagged fish based on length and uncertainties in estimated population structure. Results of a tagging experiment conducted by the Ontario Ministry of Natural Resources and Forestry (OMNRF) on yellow perch in Lake Erie was analyzed as a case study. A total of 13,694 yellow perch were tagged with PIT tags from 2009 to 2015; 322 of these were recaptured in the Ontario commercial gillnet fishery and recorded by OMNRF personnel. Different movement configurations modeling the tag-recovery data were compared, and all configurations revealed that yellow perch individuals in the western basin (MU1) exhibited relatively strong site fidelity, and individuals from the central basin (MU2 and MU3) moved within this basin, but their movements to the western basin (MU1) appeared small. Model with random effects of year and age on movement had the best performance, indicating variations in movement of yellow perch across the lake among years and age classes. This kind of model is applicable to other tagging studies to explore temporal and age-class variations while incorporating uncertainties in age classification.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0243423PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721192PMC
January 2021

Advances on liver cell-derived exosomes in liver diseases.

J Cell Mol Med 2021 Jan 27;25(1):15-26. Epub 2020 Nov 27.

Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical University, Beijing, China.

Exosomes are extracellular vesicles with diameters ranging from 30 to 150 nm, which contain several donor cell-associated proteins as well as mRNA, miRNA, and lipids and coordinate multiple physiological and pathological functions through horizontal communication between cells. Almost all types of liver cells, such as hepatocytes and Kupffer cells, are exosome-releasing and/or exosome-targeted cells. Exosomes secreted by liver cells play an important role in regulating general physiological functions and also participate in the onset and development of liver diseases, including liver cancer, liver injury, liver fibrosis and viral hepatitis. Liver cell-derived exosomes carry liver cell-specific proteins and miRNAs, which can be used as diagnostic biomarkers and treatment targets of liver disease. This review discusses the functions of exosomes derived from different liver cells and provides novel insights based on the latest developments regarding the roles of exosomes in the diagnosis and treatment of liver diseases.
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http://dx.doi.org/10.1111/jcmm.16123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810930PMC
January 2021

Effects of Shendibushen on the Expression of CD146 and its Metabolic Pathways.

Altern Ther Health Med 2020 Nov 27. Epub 2020 Nov 27.

Context: Evidence from multiple studies has revealed that it's meaningful to evaluate the clinical significance of CD146 because it's related to an early diagnosis of chronic renal failure as well as to the severity of illness and the patient's prognosis.

Objective: The current study intended to evaluate the therapeutic effects of Shendibushen on the clinical parameters of blood and urine and on fibrosis in the kidney in a rat model, using simulated renal tissue fibrosis that was surgically induced with unilateral ureteral obstruction (UUO). Also, our research team intended to analyze the metabolic pathway activated by Shendibushen both in rat and human kidneys through use of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the GeneNetwork. The aim is to discover if a connection existed between CD146 and key genes in these pathways.

Design: The research team conducted an animal study in Wistar rats.

Intervention: The rats were divided into 5 groups of 14 animals each: (1) blank control group, (2) sham control group, (3) model group, (4) Niaoduqing group, and (5) Shendibushen group. Three groups had UUO surgically induced-the model, Niaoduqing, and Shendibushen groups. The sham control group received sham surgery, and the blank control group received no surgery. The Shendibushen and Niaoduqing groups received the relevant capsules once a day at a fixed time, for a total of 28 days.

Outcome Measures: The levels of serum creatinine, blood urea nitrogen, microalbuminuria, serum soluble CD146, and urinary soluble CD146 were measured on the 14th and 28th days after modeling the rats. The degree of renal interstitial fibrosis was examined by hematoxylin and eosin (HE) staining and Masson trichrome staining. The changes at transcriptome level were obtained by target tissue sequencing. The KEGG database was used to analyze the potential pathway activated by the Shendibushen treatment. The GeneNetwork analysis was used to validate the correlation and identify the connections between CD146 and the key genes of the potential pathways.

Results: Shendibushen capsules decreased the degree of renal interstitial fibrosis in the UUO rat model and reduced the serum creatinine, blood urea nitrogen, microalbumin, serum sCD146, and urinary sCD146 significantly compared to the model group (P < .05). Upon analysis of the metabolic pathways activated by Shendibushen, the study further verified, through use of the KEGG database, that CD146 activated the nuclear factor kappa B1 (NF-κB1) and transforming growth factor beta 1 (TGF-β1)/ SMAD family member 2 (SMAD2) pathways.

Conclusions: CD146 could become an early indicator in clinical monitoring. CD146 has a function related to the NF-κB1and TGF-β1/ SMAD2 pathways under Shendibushen treatment.
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November 2020

Prognostic Significance and Related Mechanisms of Hexokinase 1 in Ovarian Cancer.

Onco Targets Ther 2020 11;13:11583-11594. Epub 2020 Nov 11.

Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, People's Republic of China.

Purpose: Ovarian cancer (OC) has the highest mortality among gynecological malignancies. Therefore, it is urgent to explore prognostic biomarkers to improve the survival of OC patients. One of the most prominent metabolic characteristics of cancer is effective glycolysis. Hexokinase 1 (HK1), as the first rate-limiting enzyme in glycolysis, is closely related to cancer progression. However, the role of HK1 in OC remains unclear.

Materials And Methods: The Cancer Genome Atlas (TCGA) database was used to detect the expression of in OC patients. The chi-squared test was performed to examine the correlations between and patients' clinical characteristics. Survival analyses were undertaken to determine the relationship between HK1 and patient survival, while the univariate/multivariate Cox model was used to evaluate the role of HK1 in patient prognosis. Gene Set Enrichment Analysis (GSEA) was performed to ascertain the related signaling pathways of HK1. RT-qPCR was implemented to validate the mRNA expression of in OC cells. MTT was used to detect cell viability after adding 2DG and knocking down in OC cells. HK1 protein expression was examined by Western blotting. Glucose uptake, lactate production, and ATP assays were undertaken following knockdown of in OC cells. Colony formation assays were performed to determine OC cell proliferation after knockdown. Transwell and wound healing assays were carried out to detect the invasion and migration of OC cells after knockdown.

Results: We found that expression was increased in OC tissues and cells, and HK1 was related to the clinical characteristics of OC patients. Survival analysis revealed that OC patients in the overexpression group had poor survival. Moreover, univariant/multivariate analyses showed that may be an independent biomarker for the poor prognosis of OC patients. OC cell viability and proliferation decreased after knockdown of . Consistently, glucose uptake, lactic acid production, ATP production, invasion, and migration were also decreased. Finally, GSEA enrichment analysis and Western blotting showed that HK1 was involved in MAPK/ERK signaling.

Conclusion: may be a biomarker for the poor prognosis of OC patients and a potential therapeutic target.
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http://dx.doi.org/10.2147/OTT.S270688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667154PMC
November 2020

Anomalous C-C Coupling on Under-Coordinated Cu (111): A Case Study of Cu Nanopyramids for CO Reduction Reaction by Molecular Modelling.

ChemSusChem 2021 Jan 24;14(2):671-678. Epub 2020 Nov 24.

School of Chemical Engineering and Advanced Materials, The University of Adelaide, South Australia, 5005, Australia.

Converting CO to high value-added C hydrocarbons by CO reduction reaction attracted attention due to higher energy density, readiness for transportation, and established utilization infrastructure. Herein, it was demonstrated that tailoring the copper catalyst morphology by forming nanopyramids offers alternative routes to promote C production. Using density functional theory calculations, five polycrystalline Cu nanopyramids with various orientations, shapes, and exposing facets were investigated. Three investigated nanopyramids favored the C production to different extents due to anomalous C-C coupling behaviors. The underlying reason for such C-C coupling was the pyramidal effect on under-coordinated Cu (111) surface from the nanopyramids. The pyramidal effect includes improved *CO adsorption, geometrically preferable sites for C-C coupling, and enhanced electron transfer. Based on these results, a C active site screening principle was developed: an extended "square" principle, which can serve as a new morphology design rule for efficient catalyst development.
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http://dx.doi.org/10.1002/cssc.202002036DOI Listing
January 2021

A biomimetic nanoenzyme for starvation therapy enhanced photothermal and chemodynamic tumor therapy.

Nanoscale 2020 Nov;12(45):23159-23165

Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, Jilin, China.

Photothermal therapy (PTT) and chemodynamic therapy (CDT) are promising therapeutic modalities with high specificity, however, a single therapeutic modality cannot maximize therapeutic efficacy. In the present study, glucose oxidase (GOx) was decorated on N-doped carbon (NC) nanoparticles (NPs) as a biomimetic nanoenzyme (NC@GOx NPs), which could promote starvation therapy enhanced PTT and CDT against tumors. GOx could decompose to cut off the supply of energy and nutrients, inducing starvation therapy, which further lowered adenosine triphosphate (ATP) levels, inducing downregulated heat shock proteins and creating a more suitable microenvironment for improving PTT efficacy. Meanwhile, the generated endogenous hydrogen peroxide (H2O2) could be converted into hydroxyl radicals to attack cancer cells. In fact, in vitro and in vivo experiments demonstrated that NC@GOx NPs could effectively kill cancer cells and eliminate tumors. This design provides a strategy for synergistic cancer therapy by using biomimetic nanoenzymes.
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http://dx.doi.org/10.1039/d0nr05097kDOI Listing
November 2020

Topotactically Transformed Polygonal Mesopores on Ternary Layered Double Hydroxides Exposing Under-Coordinated Metal Centers for Accelerated Water Dissociation.

Adv Mater 2020 Dec 13;32(52):e2006784. Epub 2020 Nov 13.

School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, SA 5005, Australia.

Layered double hydroxides (LDHs) have been recognized as potent electrocatalysts for oxygen evolution reaction (OER), but are lacking in hydrogen evolution reaction (HER) activities due to the sluggish kinetics of water dissociation in alkaline medium. Herein, aiming to simultaneously bolster the HER and OER kinetics, a metal-organic framework (MOF) mediated topotactic transformation tactic is deployed to fabricate holey ternary CoFeNi LDHs on nickel foam, exposing polygonal mesopores with atomistic edge steps and lattice defects. The optimized catalyst requires only an external voltage of 1.49 V to afford the water splitting current density of 10 mA cm apart from the superb electrolytic stability, far surpassing the benchmark Pt/C||RuO couple. More importantly, mechanistic investigations utilizing advanced spectroscopies in conjunction with density function theory (DFT) understandings unravel while the synergetic effect among under-coordinated metal centers lowers the energy barrier of water dissociation, Fe-doping enables further modulating the d-band density of states (DOS) of Co and Ni in favor of intermediates binding, thereby promoting the intrinsic HER activity. Operando Raman studies reveal negligible structural change of the LDHs during the HER process, whereas for OER the active sites can quickly turn into oxyhydroxides in the presence of lattice defects and under-coordinated metal centers.
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http://dx.doi.org/10.1002/adma.202006784DOI Listing
December 2020

Two dimensional electrocatalyst engineering via heteroatom doping for electrocatalytic nitrogen reduction.

Chem Commun (Camb) 2020 Nov 29;56(91):14154-14162. Epub 2020 Oct 29.

Key Laboratory for Advanced Ceramics and Machining Technology of Ministry of Education, Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin, 300072, China.

The electrocatalytic N reduction reaction (eNRR) - which can occur under ambient conditions with renewable energy input - became a promising synthetic pathway for ammonia (NH) and has attracted growing attention in the past few years. Some achievements have been made in the eNRR; however, there remain significant challenges to realize satisfactory NH production. Therefore, the rational design of highly efficient and durable eNRR catalysts with N[triple bond, length as m-dash]N bond activating and breaking ability is highly desirable. Two-dimensional (2D) materials have shown great potential in electrocatalysis for energy conversion and storage. Although most 2D materials are inactive toward the eNRR, they can be activated by various modification methods. Heteroatom doping engineering can impact the charge distribution and spin states on catalytic sites, therefore accelerating the dinitrogen adsorption and protonation process. This review summarises the recent research progress of heteroatom-doped 2D materials, including carbon, molybdenum disulfide (MoS) and metal carbides (MXenes), for the eNRR. In addition, some existing opportunities and future research directions in electrocatalytic nitrogen fixation for ammonia production are discussed.
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http://dx.doi.org/10.1039/d0cc05635aDOI Listing
November 2020

MiRNA-128 and MiRNA-142 Regulate Tumorigenesis and EMT in Oral Squamous Cell Carcinoma Through HOXA10.

Cancer Manag Res 2020 12;12:9987-9997. Epub 2020 Oct 12.

Department of Stomatology, Central Hospital of Taian, Taian, Shandong, People's Republic of China.

Background: Oral squamous cell carcinoma (OSCC) accounts for more than 90% of all oral cavity cancers, and the 5-year survival rate for OSCC patients remains unsatisfactory. MiRNA-128/miRNA-142 has been reported to work as a tumor suppressor in diverse tumors. However, the biological function of miR-128/miR-142 in OSCC is still unknown.

Methods: The expression of miR-128/miR-142 and homeobox A10 (HOXA10) in OSCC tissues and cells was measured by quantitative real-time polymerase chain reaction (RT-qPCR). The effects of miR-128/miR-142 or HOXA10 on proliferation, migration, invasion and apoptosis were detected by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT), transwell and flow cytometry assays, respectively. The expression levels of epithelial-mesenchymal transition (EMT)-associated proteins (E-cadherin, N-cadherin and Vimentin), proliferation-associated protein ki-67 and HOXA10 were detected by Western blot assay. The interaction between HOXA10 and miR-128/miR-142 was predicted by TargetScan, and then confirmed by dual-luciferase reporter assay.

Results: MiR-128/miR-142 was downregulated in OSCC tissues and cells. Overexpression of miR-128/miR-142 inhibited proliferation, migration, invasion and EMT and induced apoptosis in OSCC cells. HOXA10 as the target of miR-128/miR-142 was verified in OSCC cells. Knockdown of HOXA10 also repressed proliferation, migration, invasion and EMT and boosted apoptosis in OSCC cells. Upregulation of miR-128/miR-142 hindered the expression level of HOXA10, while introduction of HOXA10 weakened the effect.

Conclusion: MiR-128/miR-142 suppressed OSCC tumorigenesis and metastasis by targeting HOXA10, providing a new promising therapeutic approach for OSCC patient diagnosis and treatment.
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http://dx.doi.org/10.2147/CMAR.S250093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567577PMC
October 2020

A rare case report of immunoglobulin G4-related sclerosing mesenteritis and review of the literature.

Medicine (Baltimore) 2020 Oct;99(41):e22579

Department of Gastroenterological Surgery.

Introduction: Immunoglobulin G4 (IgG4)-related disease (IgG4-RD) is a rare autoimmune disorder involving 1 or multiple organs, most commonly the pancreas, lacrimal glands, and salivary glands. However, IgG4-related sclerosing mesenteritis (SM) involving the small-bowel mesentery is rare. Given that IgG4-related SM usually mimics the imaging characteristics of mesenteric malignancies, its preoperative diagnosis remains challenging. In addition, no specific consensus has been reached regarding the treatment of IgG4-related SM. Therefore, a better understanding of the characteristics, treatment, and prognosis of IgG-related SM is urgently needed. Herein, we report a rare case of IgG-related SM.

Patient Concerns: A 67-year-old man was admitted to our hospital after incidental detection of an abdominal mass on ultrasound imaging, although he reported being generally well. The findings on triple-phase abdominal computed tomography were highly consistent with a malignant mesenteric tumor.

Diagnoses: The hallmark histopathological features along with elevated levels of IgG4 (145 mg/dL) and imaging findings were indicative of IgG-related SM.

Interventions: The patient was treated surgically. Postoperative histopathological examinations exhibited tissue infiltration with lymphocytes and IgG4-positive plasma cells, as well as fibrosis.

Outcomes: Ten days after surgery, the patient was discharged from the hospital, and did not show any clinical sign of IgG-related SM within 1-year follow-up.

Conclusion: This case highlights the mesentery as an uncommon site of involvement as well as how early IgG-related SM can be completely asymptomatic. Thus, this study has advanced our knowledge of IgG-related SM and may improve treatments for similar conditions.
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http://dx.doi.org/10.1097/MD.0000000000022579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544369PMC
October 2020

Long non-coding RNA BACE1-AS is an independent unfavorable prognostic factor in liver cancer.

Oncol Lett 2020 Nov 8;20(5):202. Epub 2020 Sep 8.

Stem Cell and Cancer Center, First Hospital, Jilin University, Changchun, Jilin 130021, P.R. China.

Liver cancer is one of the leading causes of cancer-associated deaths with incidence rates continuously on the rise. Biomarkers are urgently required for early diagnosis and better prognostic classification, which is essential for risk stratification and optimizing treatment strategies in clinical settings. By analyzing the data extracted from The Cancer Genome Atlas database using R, the long noncoding RNA (lncRNA) β-site APP-cleaving enzyme 1 antisense ( was discovered to have both high diagnostic and prognostic values in liver cancer, which could serve as a promising biomarker in clinical settings. Precisely, lncRNA is significantly overexpressed in liver cancer and its levels vary within different subgroups, suggesting its tumorigenic role. Furthermore, higher predicts poorer overall survival and relapse-free survival outcomes. Overall, the present study demonstrated that may be involved in liver cancer progression and could serve as a promising biomarker for diagnosis and prognostic evaluation.
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http://dx.doi.org/10.3892/ol.2020.12065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491030PMC
November 2020

Gold Nanorod-Based Nanoplatform Catalyzes Constant NO Generation and Protects from Cardiovascular Injury.

ACS Nano 2020 10 30;14(10):12854-12865. Epub 2020 Sep 30.

CAS Key Laboratory of Standardization and Measurement for Nanotechnology & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Beijing 100190, China.

Cardiovascular disease is a leading cause of death, and one of the effective therapeutic strategies for cardiovascular disease is to provide a controlled, constant supply of nitric oxide (NO) in a mild manner; however, this has proved challenging in the clinic. To address this problem, we built a nitric oxide synthase (NOS)-like nanoplatform (NanoNOS) that consists of a noble metal nanoparticle core and a mesoporous silica shell and demonstrated the ability of NanoNOS to catalyze production of NO . Mechanistic studies show that the catalysis consists of a three-step reaction: the oxidation of NADPH to produce O oxidase-like activity and the subsequent dismutation of O to HO SOD-like activity, followed by HO-mediated oxidation of l-arginine to produce NO a nonenzymatic pathway. The generation of NO is precisely regulated by both the content of the NanoNOS species and the plasmon excitation. We found that NanoNOS greatly suppressed injury-driven monocyte-endothelial cell adhesion, suggesting the NanoNOS treatment could help prevent cardiovascular disease. With such a design as well as plasmon excitation that allows for controlled and constant catalytic activity, NanoNOS technology could have a variety of biomedical applications.
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http://dx.doi.org/10.1021/acsnano.0c03629DOI Listing
October 2020

Gene Expression Profiling Studies Using Microarray in Osteoarthritis: Genes in Common and Different Conditions.

Arch Immunol Ther Exp (Warsz) 2020 Sep 10;68(5):28. Epub 2020 Sep 10.

First Affiliated Hospital, Heilongjiang University of Chinese Medicine, 26 Heping Road, Xiangfang District, Harbin, Heilongjiang, People's Republic of China.

Osteoarthritis (OA), which is characterized mainly by cartilage degradation, is the most prevalent joint disorder worldwide. Although OA is identified as a major cause of joint pain, disability, and socioeconomic burden, the etiology of OA is still not clearly known. Recently, gene microarray analysis has become an efficient method for the research of complex diseases and has been employed to determine what genes and pathways are involved in the pathological process of OA. In this review, OA study results over the last decade are summarized for gene expression profiling of various tissues, such as cartilage, subchondral bone, and synovium in human OA and mouse OA models. Many differentially expressed genes, which mainly involve matrix metabolism, bone turnover, and inflammation pathways, were identified in diseased compared with "normal" tissues. Nevertheless, rare common genes were reported from studies using different tissue sources, microarray chips, and research designs. Thus, future novel and carefully designed microarray studies are required to elucidate underlying genetic mechanisms in the pathogenesis of OA as well as new directions for potential OA-targeted pharmaceutical therapies.
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http://dx.doi.org/10.1007/s00005-020-00592-4DOI Listing
September 2020

Reconciling larval and adult sampling methods to model growth across life-stages.

PLoS One 2020 21;15(8):e0237737. Epub 2020 Aug 21.

Department of Fish and Wildlife Conservation, Virginia Tech, Blacksburg, Virginia, United States of America.

Individual growth rates are intrinsically related to survival and lifetime reproductive success and hence, are key determinants of population growth. Efforts to quantify age-size relationships are hampered by difficulties in aging individuals in wild populations. In addition, species with complex life-histories often show distinct shifts in growth that cannot be readily accommodated by traditional modelling techniques. Amphibians are often characterized by rapid larval growth, cessation of growth prior to metamorphosis, and resumption of growth in the adult stage. Compounding issues of non-linear growth, amphibian monitoring programs typically sample larval and adult populations using dissimilar methods. Here we present the first multistage growth model that combines disparate data collected across life-history stages. We model the growth of the endangered Reticulated Flatwoods Salamander, Ambystoma bishopi, in a Bayesian framework, that accounts for unknown ages, individual heterogeneity, and reconciles dip-net and drift fence sampling designs. Flatwoods salamanders achieve 60% of growth in the first 3 months of life but can survive for up to 13 years as a terrestrial adult. We find evidence for marked variability in growth rate, the timing and age at metamorphosis, and maximum size, within populations. Average size of metamorphs in a given year appeared strongly dependent on hydroperiod, and differed by >10mm across years with successful recruitment. In contrast, variation in the sizes of emerging metamorphs appeared relatively constant across years. An understanding of growth will contribute to the development of population viability analyses for flatwoods salamanders, will guide management actions, and will ultimately aid the recovery of the species. Our model formulation has broad applicability to amphibians, and likely any stage-structured organism in which homogenous data cannot be collected across life-stages. The tendency to ignore stage-structure or omit non-conforming data in growth analyses can no longer be afforded given the high stakes of management decisions, particularly for endangered or at-risk populations.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0237737PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442236PMC
October 2020

C/EBPα-mediated transcriptional activation of miR-134-5p entails KPNA3 inhibition and modulates focal hypoxic-ischemic brain damage in neonatal rats.

Brain Res Bull 2020 11 16;164:350-360. Epub 2020 Aug 16.

Neonatology Department, Rizhao People's Hospital, Rizhao 276800, PR China.

Hypoxic-ischemic brain damage (HIBD) is a frequent cause of mortality and neurological handicaps in infants and children worldwide. To understand better the pathogenesis and management of HIBD, we established a HIBD model by common carotid artery ligation followed by systemic hypoxia in neonatal rats, and in other studies induced neuronal death in rat pheochromocytoma (PC12) cells by 12 h of oxygen-glucose deprivation (OGD). The level of KPNA3 declined in rats following experimental HIBD and in PC12 cells following OGD. KPNA3 overexpression protected neonatal rats against HIBD and PC12 cells against OGD-induced cell death. KPNA3 demonstrated to be the target of miR-134-5p could be activated by the transcriptional factor CCAAT/enhancer binding protein alpha (C/EBPα). The expression of miR-134-5p and C/EBPα was elevated in rats following experimental HIBD and in PC12 cells following OGD. In the parallel experiments, C/EBPα knockdown and miR-134 inhibition protected against HIBD pathology in neonatal rats and against OGD-induced neuronal death in PC12 cells. These findings reveal that the C/EBPα/miR-134-5p/KPNA3 axis mediates hypoxic-ischemic brain damage and neuronal death, thus presenting a potential therapeutic target for the treatment of human newborns at risk for HIBD.
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http://dx.doi.org/10.1016/j.brainresbull.2020.08.006DOI Listing
November 2020

GPSM2 Serves as an Independent Prognostic Biomarker for Liver Cancer Survival.

Technol Cancer Res Treat 2020 Jan-Dec;19:1533033820945817

Department of Gastrointestinal Colorectal and Anal Surgery, 74569China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.

Background And Objective: Liver cancer is a malignancy with a poor prognosis. G protein signaling modulator 2 is mainly related to cell division and cell cycle regulation. In this review, the relationship between G protein signaling modulator 2 and clinical characteristics of patients with liver cancer has been explored, especially with respect to its prognostic value.

Methods: G protein signaling modulator 2 messenger RNA expression and clinicopathological characteristics of patients with liver cancer were obtained from The Cancer Genome Atlas. The expression level of G protein signaling modulator 2 RNA-Seq was validated by using Gene Expression Omnibus. Chi-square test was performed to evaluate the relationship between G protein signaling modulator 2 expression and clinical characteristics. The threshold value of G protein signaling modulator 2 in the diagnosis of liver cancer was evaluated by a receiver-operating characteristic curve. Cox regression analysis and Kaplan-Meier curves were performed to evaluate the relationship between G protein signaling modulator 2 and liver cancer prognosis, which included overall and residual-free survival, and explored the prognostic value of G protein signaling modulator 2. Liver cancer survival analyses were validated by using the data of G protein signaling modulator 2 RNA-Seq from the International Cancer Genome Consortium.

Results: The expression level of G protein signaling modulator 2 messenger RNA was remarkably higher in liver cancer than that in healthy tissues ( < 2.2 × e), which was also validated by data from the GSE14520 database. In addition, high G protein signaling modulator 2 expression significantly correlated with histological grade ( = .020), vital status ( < .001), clinical ( = .001), and T stage ( = .001). The receiver-operating characteristic curves showed G protein signaling modulator 2 to be an advantageous diagnostic molecule for liver cancer (area under curve = 0.893). Furthermore, the results of Cox analysis and Kaplan-Meier curves suggested that the upregulation of G protein signaling modulator 2 expression is linked to poor prognosis and G protein signaling modulator 2 messenger RNA could be an independent predictor for liver cancer, which was validated by data from the International Cancer Genome Consortium database.

Conclusions: G protein signaling modulator 2 messenger RNA was overexpressed in liver cancer, and G protein signaling modulator 2 is an independent prognostic factor. G protein signaling modulator 2 is expected to be a treatment target for cancer.
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http://dx.doi.org/10.1177/1533033820945817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440740PMC
August 2020

Discovering Biomarkers in Peritoneal Metastasis of Gastric Cancer by Metabolomics.

Onco Targets Ther 2020 27;13:7199-7211. Epub 2020 Jul 27.

Department of Gastrointestinal Surgery, First Hospital of Jilin University, Changchun, Jilin Province 130000, People's Republic of China.

Background And Objective: Metabolomics has recently been applied in the field of oncology. In this study, we aimed to use metabolomics to explore biomarkers in peritoneal metastasis of gastric cancer.

Methods: Peritoneal lavage fluid (PLF) of 65 gastric cancer patients and related clinical data were collected from the First Hospital of Jilin University. The metabolic components were identified by liquid chromatography-mass spectrometry (LC-MS). Total ion current (TIC) spectra, principal component analysis (PCA), and the Student's -test were used to identify differential metabolites in PLF. A support vector machine (SVM) was used to screen the differential metabolites in PLF with a weight of 100%. Cluster analysis was used to evaluate the similarity between samples. Receiver operating characteristic (ROC) curve analysis was used to assess the diagnostic ability of the metabolites. Univariate and multivariate logistic regression analyses were used to identify potential risk factors for peritoneal metastasis of gastric cancer.

Results: We found the differential levels of PLF metabolites by LC-MS, TIC spectra, PCA and the -test. Cluster analysis showed the co-occurrence of metabolites in the peritoneal metastasis group (p<0.05). ROC analysis showed the diagnostic ability of metabolites (p<0.05). Univariate and multivariate logistic regression analyses showed the potential independent risk factors for peritoneal metastasis in gastric cancer patients (p<0.05).

Conclusion: Through the statistical analysis of metabolomics, we found that TG (54:2), G3P, α-aminobutyric acid, α-CEHC, dodecanol, glutamyl alanine, 3-methylalanine, sulfite, CL (63:4), PE-NMe (40:5), TG (53:4), retinol, 3-hydroxysterol, tetradecanoic acid, MG (21:0/0:0/0:0), tridecanoic acid, myristate glycine and octacosanoic acid may be biomarkers for peritoneal metastasis of gastric cancer.
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http://dx.doi.org/10.2147/OTT.S245663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7394602PMC
July 2020