Publications by authors named "Yan Hu"

997 Publications

Development and Validation of Predicting Nomograms for Craniopharyngioma: A Retrospective, Multiple-Center, Cohort Study.

Front Oncol 2021 12;11:691288. Epub 2021 Jul 12.

Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Craniopharyngiomas (CPs) are benign tumors arising from the sellar region. However, little is known about their clinical features and long-term recurrence due to low morbidity and the lack of large cohort studies. Thus, we aimed to develop nomograms to accurately predict the extent of resection and tumor recurrence using clinical parameters. A total of 545 patients diagnosed with CP between 2009 and 2019 were examined: 381 in the development cohort and 164 in the validation cohort. Least absolute shrinkage and selection operator (LASSO) and Cox regression analyses were performed to establish two nomograms. Receiver operating characteristic (ROC) curves, calibration curves, decision curve analysis (DCA) and Kaplan-Meier (KM) curves were used to evaluate their predictive performance and discriminative power, respectively, in the two cohorts. In addition, the EORTC QLQ-BN20 questionnaire was used to assess neuropsychological status in the follow-up. In the development cohort, the area under the curve (AUC) and C-index were 0.760 and 0.758, respectively, for predicting the extent of resection and 0.78 and 0.75, respectively, for predicting 3-year progression-free survival (PFS) and 5-year PFS. Additionally, the model had a predictive accuracy of 0.785. Both nomograms showed acceptable discrimination in the two cohorts. Moreover, DCA demonstrated excellent clinical benefits from the two nomograms. Finally, participants were classified into two distinct risk groups according to the risk score, and an online calculator was created for convenient clinical use. During long term follow-up, hypothyroidism (77.61%) and hypocortisolism (76.70%) were the most common endocrine dysfunction after surgery and significant deficits were observed concerning visual disorder, motor dysfunction and seizures in the recurrent groups. In particular, better quality of life was associated with gross total resection (GTR), postoperative radiation, anterior interhemispheric (AI) approach and transsphenoidal approach. To our knowledge, these are the first nomograms based on a very large cohort of patients with CP that show potential benefits for guiding treatment decisions and long-term surveillance. The current study demonstrated the online calculator serve as the practical tool for individual strategies based on the patient's baseline characteristics to achieve a better prognosis.
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http://dx.doi.org/10.3389/fonc.2021.691288DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312552PMC
July 2021

Comparison of the differences between web-based and traditional questionnaire surveys in pediatrics: Comparative Survey Study.

J Med Internet Res 2021 Jul 27. Epub 2021 Jul 27.

Department of Child Health Care, Children's Hospital of Chongqing Medical University, Chongqing, P.R. China., No.136, 2nd Zhongshan Road, Yuzhong District, Chongqing, 400014, China., Chongqing, CN.

Background: The web-based survey is a novel method for data capture. Some studies have applied web-based survey in pediatrics, but few of them have reported the data on the differences between it and traditional questionnaire surveys.

Objective: The objective of our study was to evaluate the internal consistency of web-based survey and to compare it with traditional questionnaire survey in pediatrics.

Methods: A convenience sample of caregivers were invited to participate in the survey on the feeding patterns and their children's eating behavior if their children were aged 2 to 7 years. A web-based survey and a traditional questionnaire survey were carried out between October 2018 and July 2019. A total of 1085 caregivers were involved in this study, and they were divided into 3 groups based on the methods and sources: (1) web-based survey from web source, (2) web-based survey from hospital source, (3) traditional questionnaire survey from hospital source. The data were then compared and analyzed.

Results: A total of 735 caregivers participated in the web-based survey and 350 caregivers participated in the traditional questionnaire survey, and 816 cases were then included in the analyses after data processing. The effective rate of the web-based survey was 70.1% and the completeness rate of the traditional questionnaire survey was 86.0%. There were no significant differences between web-based surveys from different sources. However, the demographic characteristics between the web-based and traditional questionnaire surveys were significantly different, mainly in age and caregivers (χ²=16.509, P=.002; χ²=111.464, P<.001). Caregivers of children aged 2 to 3 years and grandparents were more likely to respond to the web-based survey. Age-specific stratified analysis then showed the score of "monitoring" and reporting rate of "poor appetite" in children aged 2 to 3 years were significantly higher in the web-based survey compared to the traditional questionnaire survey after adjusting for demographic characteristics.

Conclusions: Web-based survey could be a feasible tool in pediatric studies. However, differences in demographic characteristics and their possible impact on the results should be considered in the analyses.

Clinicaltrial:
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http://dx.doi.org/10.2196/30861DOI Listing
July 2021

Analysis of risk factors of PICC-related bloodstream infection in newborns: implications for nursing care.

Eur J Med Res 2021 Jul 23;26(1):80. Epub 2021 Jul 23.

Department of Nursing, Children's Hospital, School of Medicine, Zhejiang University, No. 3333 Binsheng Road, Binjiang District, Hangzhou City, Zhejiang Province, China.

Background: It is necessary to analyze the characteristics and risk factors of catheter-related bloodstream infection (CRBSI) in newborns with peripherally inserted central catheter (PICC).

Methods: Newborns undergoing PICC catheterization in the neonatal department of our hospital from January 1, 2020 to January 31, 2021 were included. The characteristics of newborns with and without CRBSI newborns were compared and analyzed. Logistic regression analyses were performed to evaluate the risk factors of CRBSI in newborns with PICC.

Results: Three hundred eighty-six newborns with PICC were included, of whom 41 newborns had the CRBSI, the incidence of CRBSI in newborns with PICC was 10.62%. There were significant differences regarding the birth weight, durations of PICC stay, 5-min Apgar score, site of PICC insertion of PICC between CRBSI and no CRBSI group (all P < 0.05), and there were no significant differences regarding the gender, gestational age, cesarean section, mechanical ventilation and length of hospital stay between CRBSI and no CRBSI group (all P > 0.05). Escherichia coli (26.08%) and Staphylococcus aureus (23.92%) were the most common CRBSI pathogens in newborns with PICC. Logistic regression analysis indicated that birth weight ≤ 1500 g (OR 1.923, 95% CI 1.135-2.629), durations of PICC stay ≥ 21 days (OR 2.077, 95% CI 1.024-3.431), 5-min Apgar score ≤ 7 (OR 2.198, 95% CI 1.135-3.414) and femoral vein insertion of PICC (OR 3.044, 95% CI 1.989-4.306) were the independent risk factors of CRBSI in neonates with PICC (all P < 0.05).

Conclusion: For newborns with low birth weight, longer durations of PICC stay and femoral vein PICC insertion, they may have higher risks of CRBSI, and medical staff should take targeted measures to reduce the development of CRBSI.
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http://dx.doi.org/10.1186/s40001-021-00546-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299687PMC
July 2021

A safe and effective mucosal RSV vaccine in mice consisting of RSV phosphoprotein and flagellin variant.

Cell Rep 2021 Jul;36(3):109401

Vaccine and Immunology Research Center, Translational Medical Research Institute, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; Mucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. Electronic address:

Respiratory syncytial virus (RSV) is a major cause of serious acute lower respiratory tract infection in infants and the elderly. The lack of a licensed RSV vaccine calls for the development of vaccines with other targets and vaccination strategies. Here, we construct a recombinant protein, designated P-KFD1, comprising RSV phosphoprotein (P) and the E.-coli-K12-strain-derived flagellin variant KFD1. Intranasal immunization with P-KFD1 inhibits RSV replication in the upper and lower respiratory tract and protects mice against lung disease without vaccine-enhanced disease (VED). The P-specific CD4 T cells provoked by P-KFD1 intranasal (i.n.) immunization either reside in or migrate to the respiratory tract and mediate protection against RSV infection. Single-cell RNA sequencing (scRNA-seq) and carboxyfluorescein succinimidyl ester (CFSE)-labeled cell transfer further characterize the Th1 and Th17 responses induced by P-KFD1. Finally, we find that anti-viral protection depends on either interferon-γ (IFN-γ) or interleukin-17A (IL-17A). Collectively, P-KFD1 is a promising safe and effective mucosal vaccine candidate for the prevention of RSV infection.
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http://dx.doi.org/10.1016/j.celrep.2021.109401DOI Listing
July 2021

Redox-responsive magnetic nanovectors self-assembled from amphiphilic polymer and iron oxide nanoparticles for a remotely targeted delivery of paclitaxel.

J Mater Chem B 2021 Jul 14. Epub 2021 Jul 14.

Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing University, Chongqing 400044, China.

To reduce the side effect of paclitaxel and enhance accumulation at the tumor site, a novel redox-responsive nanovector with excellent biocompatibility based on disulfide-linked amphiphilic polymer and magnetic nanoparticle was prepared. The system would realize PTX release due to breakage of the disulfide bond when being targeted to the tumor site by the external magnetic field. The nanovector significantly improved endocytosis and enhanced accumulation at the tumor site, with an effective inhibition of tumor cells in vitro and in vivo.
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http://dx.doi.org/10.1039/d1tb00991eDOI Listing
July 2021

Enhanced biocompatibility and osteogenic differentiation of mesenchymal stem cells of titanium by Sr-Ga clavate double hydroxides.

J Mater Chem B 2021 Jul 14. Epub 2021 Jul 14.

Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.

To improve in vivo osseointegration of pure titanium implant, Sr-Ga clavate double hydroxide (CDH) coating was grown in situ on a titanium (Ti) substrate with simple hydrothermal and calcination treatments at 500 °C. The obtained coating on the Ti substrate (Ti-CDH and Ti-CDH500) was researched by scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive spectroscopy (EDS). Ti-CDH exhibited a sustained release profile of metal ions and maintained a slightly alkaline environment. The CDH coating was beneficial for osteogenic differentiation of mesenchymal stem cells (MSCs), which were reflected by the results of cellular assays, including alkaline phosphatase activity (ALP), cell mineralization capability (ARS), and osteogenesis-related gene expression. Besides, Ti-CDH could effectively improve the autophagic levels in MSCs, which further promoted osteogenic differentiation of MSCs. Hence, the Ti surface with Sr-Ga CDH modification supplied a simple and effective strategy to design biomaterials for bone generation.
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http://dx.doi.org/10.1039/d1tb00805fDOI Listing
July 2021

Effects of Paired Associative Stimulation on Metabolites in Ischemia Stroke Rats Model as Studied by Nuclear Magnetic Resonance Spectrum.

Neurochem Res 2021 Sep 7;46(9):2495-2504. Epub 2021 Jul 7.

Department of Rehabilitation Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, No. 1095, Jiefang Avenue, Qiaokou District, Wuhan City, 430000, Hubei Province, China.

Paired associated stimulation (PAS) has been confirmed to play a role in motor recovery after stroke, but the underlying mechanism has not been fully elucidated. In this study, we employed a comprehensive battery of measurements, including behavioral test, electrophysiology and H-NMR approaches, to investigate the therapeutic effects of PAS in rat model of cerebral ischemia and its underlying mechanism. Rats were randomly divided into a transient middle cerebral artery occlusion group (tMCAO group), a tMCAO + PAS group (PAS group), and a sham group. PAS was applied over 7 consecutive days in PAS group. The behavioral function of rats was evaluated by modified Garcia Scores and Rota-rod test. Electrophysiological changes were measured by motor evoked potentials (MEP). Metabolic changes of ischemic penumbra were detected by H-NMR. After PAS intervention, the performances on Rota-rod test and Garcia test improved and the amplitude of MEP increased significantly. The gamma-aminobutyric acid (GABA) in penumbra cortex was decreased significantly, whereas the glutamate showed the opposite changes. The results suggested that post-stroke recovery promoted by PAS may be related to the metabolites alteration in ischemic penumbra and also regulate the excitability of motor cortex.
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http://dx.doi.org/10.1007/s11064-021-03388-wDOI Listing
September 2021

Effectiveness of omalizumab in patients with severe allergic asthma: A retrospective study in China.

Respir Med 2021 Jun 29;186:106522. Epub 2021 Jun 29.

Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Background: We conducted the first real-world study of treatment with omalizumab, a humanized monoclonal anti-immunoglobulin E antibody, in Chinese patients with severe allergic asthma.

Objective: The primary objective was the steroid-sparing effect of omalizumab after 12 and 16 weeks of treatment. Characteristics of the patient population, treatment patterns, response rate, and other measures of therapeutic effectiveness were also reported.

Methods: This nationwide, retrospective, real-world study was conducted in patients with severe allergic asthma who were treated with omalizumab in China. Data, including demographics, Asthma Control Test (ACT) and laboratory and lung function test results, and omalizumab use information, were extracted from patient records collected as part of a previously conducted real-world survey (Asthma Group of the Respiratory Disease Society of the Chinese Medical Association).

Results: In total, 139 patient records were included; 131 and 118 patients remained on treatment at the ≥12- and ≥16-week time points, respectively. The mean ± standard deviation age and median asthma duration (interquartile range) were 47.4 ± 14.3 and 7 (4, 15) years, respectively; 75.6% of patients had a history of allergic disease. Reductions (versus baseline) in inhaled corticosteroid/long-acting β2 agonists or oral corticosteroids were reported in 61.1% and 63.6% of patients at ≥12 and ≥ 16 weeks, respectively. There were significant improvements in ACT scores (6.08, P < .001) and nitric oxide fraction in exhaled air (-13.0, P = .01) from baseline. Multivariate analysis revealed that age and allergic medical history were predictors of omalizumab treatment response. No serious adverse events were reported.

Conclusion: Real-world omalizumab treatment was efficacious and well-tolerated in Chinese patients with severe allergic asthma.
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http://dx.doi.org/10.1016/j.rmed.2021.106522DOI Listing
June 2021

R-loops mediate transcription-associated formation of human rDNA secondary constrictions.

J Cell Biochem 2021 Jul 5. Epub 2021 Jul 5.

Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, China.

The ribosomal gene DNA (rDNA) often forms secondary constrictions in the chromosome; however, the molecular mechanism involved remains poorly understood. Here, we report that occurrence of rDNA constriction was increased in the chromosomes in human cancer cell lines compared with normal cells and that decondensed rDNA was significantly enhanced after partial inhibition of rDNA transcription. rDNA transcription was found during the S phase when replication occurred, and thus, DNA replication inhibitors caused constriction formation through hindering rDNA transcription. Inhibition of ataxia ATR (telangiectasia-mutated and RAD3-related) induced rDNA constriction formation. Replication stress or transcription inhibition increased R-loop formation. Topoisomerase I and RNase H1 suppressed secondary constriction formation. These data demonstrate that transcription stress causes the accumulation of stable R-loops (RNA-DNA hybrid) and subsequent constriction formation in the chromosomes.
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http://dx.doi.org/10.1002/jcb.30074DOI Listing
July 2021

Targeting JAK/STAT signaling pathways in treatment of inflammatory bowel disease.

Inflamm Res 2021 Jul 1;70(7):753-764. Epub 2021 Jul 1.

Research and Teaching Department of Comparative Medicine, Dalian Medical University, Dalian, 116044, China.

Janus kinase/signal transduction and transcriptional activator (JAK/STAT) signaling pathway is a transport hub for cytokine secretion and exerts its effects. The activation of JAK/STAT signaling pathway is essential for the regulation of inflammatory responses. Inappropriate activation or deletion of JAK/STAT signaling pathway is the initiator of the inflammatory response. JAK/STAT signaling pathway has been demonstrated to be involved in the process of innate and adaptive immune response to inflammatory bowel disease (IBD). In this review, we discuss the role of the JAK/STAT signaling pathway in the regulation of different cells in IBD, as well as new findings on the involvement of the JAK/STAT signaling pathway in the regulation of the intestinal immune response. The current status of JAK inhibitors in the treatment of IBD is summarized as well. This review highlights natural remedies that can serve as potential JAK inhibitors. These phytochemicals may be useful in the identification of precursor compounds in the process of designing and developing novel JAK inhibitors.
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http://dx.doi.org/10.1007/s00011-021-01482-xDOI Listing
July 2021

Comparative Metagenomic Analysis of Chicken Gut Microbial Community, Function, and Resistome to Evaluate Noninvasive and Cecal Sampling Resources.

Animals (Basel) 2021 Jun 9;11(6). Epub 2021 Jun 9.

Poultry Institute, Chinese Academy of Agricultural Science, Yangzhou 225000, China.

When conducting metagenomic analysis on gut microbiomes, there is no general consensus concerning the mode of sampling: non-contact (feces), noninvasive (rectal swabs), or cecal. This study aimed to determine the feasibility and comparative merits and disadvantages of using fecal samples or rectal swabs as a proxy for the cecal microbiome. Using broiler as a model, gut microbiomes were obtained from cecal, cloacal, and fecal samples and were characterized according to an analysis of the microbial community, function, and resistome. Cecal samples had higher microbial diversity than feces, while the cecum and cloaca exhibited higher levels of microbial community structure similarity compared with fecal samples. Cecal microbiota possessed higher levels of DNA replicative viability than feces, while fecal microbiota were correlated with increased metabolic activity. When feces were excreted, the abundance of antibiotic resistance genes like and decreased, but some antibiotic genes became more prevalent, such as , , and . Interestingly, was a dominant bacterial genus in feces that led to differences in microbial community structure, metabolism, and resistome. In conclusion, fecal microbiota have limited potential as a proxy in chicken gut microbial community studies. Thus, feces should be used with caution for characterizing gut microbiomes by metagenomic analysis.
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http://dx.doi.org/10.3390/ani11061718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228302PMC
June 2021

The transcription factor TEAD4 enhances lung adenocarcinoma progression through enhancing PKM2 mediated glycolysis.

Cell Biol Int 2021 Jun 30. Epub 2021 Jun 30.

Department of Respiratory, The First People's Hospital of Zigong City, Zigong, Sichuan, China.

Lung adenocarcinoma (LUAD) is a deadly disease with a hallmark of aberrant metabolism. TEA domain 4 (TEAD4) is involved in the progression of several forms of cancer including LUAD. However, the role of TEAD4 in LUAD glucose metabolism is rarely reported as well as its potential mechanisms. Pyruvate kinase isozymes M2 (PKM2), the key regulatory enzymes in glycolysis, was predicted to be a target for TEAD4 by bioinformatics analysis. Thus, we aimed to explore whether TEAD4/PKM2 axis was related to LUAD glucose metabolism and malignant phenotype. The expression level of TEAD4 and PKM2 was measured by quantitative real-time PCR and Western blot. Luciferase reporter assay were employed to verify the effect of TEAD4 on PKM2 promoter as well as TEAD4/PKM2 axis on reporter activity of hypoxia inducible factor-1α (HIF-1α). Glycolysis was investigated according to glucose consumption, lactate production and the extracellular acidification rate. The present study indicated that TEAD4 and PKM2 were upregulated in LUAD and closely related to prognosis. Mechanistic investigations identified that TEAD4 played a key role as a transcription factor and promoted PKM2 transcription and expression, which further altered the reporter activity of HIF-1α and upregulated HIF-1α-targeted glycolytic genes glucose transporter-1 and hexokinase II. Functional assays revealed that TEAD4 and PKM2 affected glycolytic and 2-DG blocked the positive function of TEAD4 and PKM2 on glycolytic. Besides, TEAD4/PKM2 axis affects LUAD cell viability, apoptosis, migration, and invasion. Together, these data provided evidence that both TEAD4 and PKM2 were poor prognosticator. Targeting TEAD4/PKM2 axis might be an effective therapeutic strategy for LUAD.
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http://dx.doi.org/10.1002/cbin.11654DOI Listing
June 2021

Can the BMI-based dose regimen be used to reduce injection activity and to obtain a constant image quality in oncological patients by F-FDG total-body PET/CT imaging?

Eur J Nucl Med Mol Imaging 2021 Jun 29. Epub 2021 Jun 29.

Department of Nuclear Medicine, Zhongshan Hospital affiliated Fudan University, 180 Fenglin Road, Shanghai, 200032, China.

Purpose: PET image quality is influenced by the patient size according to the current guideline. The study aimed to propose an optimized dose regimen to yield a constant image quality independent of patient habitus to meet the clinical needs.

Methods: A first patient cohort of 78 consecutive oncological patients (59.7 ± 13.7 years) who underwent a total-body PET/CT scan were retrospectively enrolled to develop the regimen. The patients were randomly distributed in four body mass index (BMI) groups according to the World Health Organization (WHO) criteria. The liver SNR (signal-to-noise ratio, SNR) was obtained by manually drawing regions of interest (ROIs) and normalized (SNR) by the product of injected activity and acquisition time. Fits of SNR against different patient-dependent parameters were performed to determine the best correlating parameter and fit method. A qualitative assessment on image quality was performed using a 5-point Likert scale to determine the acceptable threshold of SNR. Thus, an optimized regimen was proposed and validated by a second patient cohort consisted of prospectively enrolled 38 oncological patients.

Results: The linear fit showed SNR had the strongest correlation (R = 0.69) with the BMI than other patient-dependent parameters and fit method. The qualitative assessment indicated a SNR value of 14.0 as an acceptable threshold to achieve sufficient image quality. The optimized dose regimen was determined as a quadratic relation with BMI: injected activity (MBq) = 39.2 (MBq)/(- 0.03*BMI + 1.49). In the validation study, the SNR no longer decreased with the increase of BMI. There was no significant difference of the image quality regarding the value of SNR between different BMI groups (p > 0.05). In addition, the injected activity was reduced by 75.6 ± 2.9%, 72.1 ± 4.0%, 67.1 ± 4.4%, and 64.8 ± 3.5% compared with the first cohort for the four BMI groups, respectively.

Conclusion: The study proposed a quadratic relation between the F-FDG injected activity and the patient's BMI for total-body F-FDG PET imaging. In this regimen, the image quality can maintain in a constant level independent of patient habitus and meet the clinical requirement with a reduced injected activity.
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http://dx.doi.org/10.1007/s00259-021-05462-5DOI Listing
June 2021

Laparoscopic Radical Hysterectomy Results in Higher Recurrence Rate Versus Open Abdominal Surgery for Stage IB1 Cervical Cancer Patients With Tumor Size Less Than 2 Centimeter: A Retrospective Propensity Score-Matched Study.

Front Oncol 2021 10;11:683231. Epub 2021 Jun 10.

Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China.

Objective: To compare the oncologic outcomes between laparoscopic and open radical hysterectomy in patients with stage IB1 cervical cancer lesion less than 2 cm.

Methods: Patients diagnosed FIGO (2009) stage IB1 (tumor diameter <2 cm) and underwent radical hysterectomy in our hospital between March 2008 and November 2018 were studied. A propensity-matched comparison (1:2) was conducted to minimize selection biases. Demographic and baseline oncologic characteristics were balanced between groups. Overall survival (OS) and disease-free survival (DFS) were assessed using the Kaplan-Meier model, along with univariable and multivariable regression analysis.

Results: A total of 261 patients were enrolled in this study after propensity-matching, with 174 in the open group and 87 in the laparoscopic group. Disease relapsed in seven patients in laparoscopy group, and the recurrence rate was 8.0% (7/87). There were eight patients underwent abdominal radical hysterectomy experienced recurrence, and the recurrence rate was 4.6% (8/174). The multivariate analysis model revealed that laparoscopic operation was associated with higher risk of recurrence than abdominal radical hysterectomy (HR, 3.789; 95% CI, 1.143-12.559; = 0.029). There were five patients or 2.9% (5/174) died in open surgery group and the corresponding percentage in laparoscopy group was 2.3% (2/87). No difference was found in OS between the two groups (HR, 1.823; 95% CI, 0.2673-12.44; log-rank = 0.5398). All the recurrence occurred within two years after operation in the laparoscopy group, among which pelvic recurrence (85.7%) was dominant.

Conclusion: Traditional laparotomy radical hysterectomy has a lower recurrence rate when compared with laparoscopic operation in those cervical cancer patients with a foci diameter less than 2 cm. However, no detrimental effect on survival was found in minimal invasive operation group. Further multi-center prospective trials are needed to confirm our results on a large scale.
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http://dx.doi.org/10.3389/fonc.2021.683231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222693PMC
June 2021

Metal-free tandem carbene N-H insertions and C-C bond cleavages.

Chem Sci 2020 Nov 10;12(2):803-811. Epub 2020 Nov 10.

Shaanxi Key Laboratory of Chemical Additives for Industry, College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology Xi'an 710021 China

A metal-free C-H [5 + 1] annulation reaction of 2-arylanilines with diazo compounds has been achieved, giving rise to two types of prevalent phenanthridines highly selective C-C cleavage. Compared to the simple N-H insertion manipulation of diazo, this method elegantly accomplishes a tandem N-H insertion/SAr/C-C cleavage/aromatization reaction, and the synthetic utility of this new transformation is exemplified by the succinct syntheses of trisphaeridine and bicolorine alkaloids.
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http://dx.doi.org/10.1039/d0sc05763kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178978PMC
November 2020

Efficacy of montmorillonite and vitamin AD combined with zinc preparation in children with diarrheal disease and its effect on inflammatory factors.

Am J Transl Res 2021 15;13(5):5428-5435. Epub 2021 May 15.

Department of Child Health Care, Anhui Children's Hospital Hefei 230001, Anhui Province, China.

Objective: To investigate the effect of montmorillonite and vitamin AD combined with zinc preparation in children with diarrheal disease and its effect on inflammatory factors.

Methods: A total of 156 children with diarrheal diseases admitted to our hospital from January 2018 to January 2020 were enrolled and divided into two groups (n=78 for each) using random number table. The control group (CG) was treated with montmorillonite, and the observation group (OG) was additionally treated with vitamin AD and zinc. Both groups completed 7 d of treatment and 3 months of follow-up to compare the efficacy rate, time to onset of symptom relief, inflammatory factor levels, T-lymphocyte levels, adverse drug reactions, and relapse rate.

Results: The efficacy rate of 7 d treatment in the OG was 94.87%, higher than that of 56.41% in the CG ( < 0.05). After intervention, the OG had normal frequency of defecation, shorter time for stool to return to normal appearance, shorter duration of antiemetic, antipyretic, antidiarrheal treatment and shorter hospital stay than those of the CG ( < 0.05). The OG exhibited lower levels of CRP, TNF-α, NO, and PCT and higher levels of SOD than the CG ( < 0.05). The OG had lower levels CD3 and CD4 at 7 d after treatment, lower CD8 levels and higher CD4/CD8 levels than the CG ( < 0.05). The relapse rate at 6 months after treatment in the OG was lower than that in the CG ( < 0.05).

Conclusion: The combination of montmorillonite, vitamin AD and zinc preparation can achieve higher short-time efficacy, shorten the time of disappearance of symptoms, reduce the level of inflammatory factors, and improve the level of T-lymphocytes, without increasing the incidence of adverse drug reactions.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205834PMC
May 2021

Literature review and report of three cases of Dubin-Johnson syndrome related to ABCC2 gene mutations in children.

Am J Transl Res 2021 15;13(5):4477-4488. Epub 2021 May 15.

Department of Traditional Chinese Medicine, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health Beijing 100045, China.

Objective: The aim of the present study was to analyze the clinical features of Dubin-Johnson syndrome (DJS) related to ABCC2 gene mutations in children and to review the relevant literature to improve understanding of this type of genetic disease and reduce misdiagnosis.

Methods: Three children with clinically suspected DJS who were treated at Beijing Children's Hospital of Capital Medical University between 2017 and 2020 were enrolled in the study. The target genes were captured and sequenced using GenCap target gene capture technology and a new generation of high-throughput sequencing technology (Beijing Mykino Company). The clinical and genetic characteristics were analyzed and summarized.

Results: Two of the cases were female and one was male. All three cases were in early infancy and in good general health. Case 1 was complicated with unilateral hypertrophy, Case 2 was complicated with pneumonia, anemia, myocardial injury, and bilateral inguinal hernia, and Case 3 was complicated with patent foramen ovale and a ventricular septal defect. In all three cases, total bilirubin was elevated, with the main increase being in direct bilirubin (DBIL) and varying degrees of elevated alanine aminotransferase (ALT), γ-glutamyl transferase (GGT), and total bile (TBA). Genetic testing indicated that there were seven gene mutations in ABCC2, two mutation sites of which had not been reported previously.

Conclusion: The clinical manifestations of DJS are non-specific and are mainly characterized by elevated DBIL. Some children might have different degrees of hepatic function abnormality and cholestasis. Due to the lack of serological markers, the diagnosis of DJS is difficult, but genetic testing, along with the formation of pedigree analysis and verification, could be used for accurate diagnosis. Novel mutations might enrich the spectrum of ABCC2 gene mutation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205816PMC
May 2021

Sinapic Acid Reduces Oxidative Stress and Pyroptosis via Inhibition of BRD4 in Alcoholic Liver Disease.

Front Pharmacol 2021 4;12:668708. Epub 2021 Jun 4.

Department of Pharmacy, The Second Hospital of Dalian Medical University, Dalian, China.

Alcoholic liver disease (ALD) is one of the main causes of death in chronic liver disease. Oxidative stress and pyroptosis are important factors leading to ALD. Bromodomain-containing protein 4 (BRD4) is a factor that we have confirmed to regulate ALD. As a phenolic acid compound, sinapic acid (SA) has significant effects in antioxidant, anti-inflammatory and liver protection. In this study, we explored whether SA regulates oxidative stress and pyroptosis through BRD4 to play a protective effect in ALD. Male C57BL/6 mice and AML-12 cells were used for experiments. We found that SA treatment largely abolished the up-regulation of BRD4 and key proteins of the canonical pyroptosis signalling in the liver of mice fed with alcohol, while conversely enhanced the antioxidant response. Consistantly, both SA pretreatment and BRD4 knockdown inhibited oxidative stress, pyroptosis, and liver cell damage . More importantly, the expression levels of BRD4 and pyroptosis indicators increased significantly in ALD patients. Molecule docking analysis revealed a potent binding of SA with BRD4. In conclusion, this study demonstrates that SA reduces ALD through BRD4, which is a valuable lead compound that prevents the ALD process.
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http://dx.doi.org/10.3389/fphar.2021.668708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212038PMC
June 2021

Inhibition of SDF-1/CXCR4 Axis to Alleviate Abnormal Bone Formation and Angiogenesis Could Improve the Subchondral Bone Microenvironment in Osteoarthritis.

Biomed Res Int 2021 28;2021:8852574. Epub 2021 May 28.

Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

The pathogenesis of the osteoarthritis (OA) is complex. Abnormal subchondral bone metabolism is an important cause of this disease. Further understanding on the pathology of the subchondral bone in OA may provide a new therapy. This research is about to investigate the role of SDF-1 in the subchondral bone during the pathological process of OA. In vitro, Transwell was used to test the migratory ability of bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs). Western blot presented the protein level after SDF-1 treatment in BMSCs and HUVESs. Alizarin red was used to assess the ability of osteogenic differentiation. To inhibit SDF-1 signaling pathway in vivo, AMD3100 (SDF-1 receptor blocker) was continuously delivered via miniosmotic pump for 4 weeks in mice after performing anterior cruciate ligament transaction surgery. Micro-CT, histology staining, immunofluorescence, immunohistochemistry, and TRAP staining were used to assess the role of SDF-1 on osteogenesis and angiogenesis in the subchondral bone. Our results showed that SDF-1 could recruit BMSCs, activate the p-ERK pathway, and enhance osteogenic differentiation. SDF-1 promoted the ability of proliferation, migration and tube formation of HUVECs by activating the ERK and AKT signaling pathways. In an animal study, inhibition of SDF-1/CXCR4 axis could significantly reduce subchondral osteogenesis differentiation and H-type vessel formation. Furthermore, the AMD3100-treated group showed less cartilage destruction and bone resorption. Our research shows that SDF-1 alters the microenvironment of the subchondral bone by promoting osteoid islet formation and abnormal H-type angiogenesis in the subchondral bone, resulting in articular cartilage degeneration.
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http://dx.doi.org/10.1155/2021/8852574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179778PMC
May 2021

PEG-Induced Controllable Thin-Thickness Gradient and Water Retention: A Simple Way to Programme Deformation of Hydrogel Actuators.

Macromol Rapid Commun 2021 Jul 15;42(14):e2000749. Epub 2021 Jun 15.

The Collaborative Innovation Center for Eco-Friendly and Fire-Safety Polymeric Materials(MoE), National Engineering Laboratory of Eco-Friendly Polymeric Materials (Sichuan), State Key Laboratory of Polymer Materials Engineering, College of Chemistry, Sichuan University, Chengdu, 610064, China.

Building the differential growth through the thickness is a promising and challenging approach to design the morphing structures of hydrogel actuators. Besides retaining the size of the hydrogel actuators under environmental stimuli still remains a big challenge. Herein, a facile and universal approach is developed to address both issues by introducing PEG during the polymerization of N-isopropylacrylamide (NIPAm) via one step method using asymmetric mold. Both composition gradient and pore gradient are obtained in micro level along the thickness direction of the final hydrogel, while thin-thickness gradient in macro level. The thickness gradient and water retention can be controllably adjusted by changing PEG concentration. The introduction of PEG effectively improves both responsive and non-shrunken performance by the interaction with PNIPAm. The resultant anisotropic PNIPAm/PEG hydrogel respond quickly and reach maximum deformation (360°) within 10 s at low temperature (40 °C). The various 3D shape and biomimetic movement can be programmed by simply controlling the PEG concentration and mold shape. This strategy can provide new insights into the design intelligent soft materials with 3D morphing for bioinspired and biomedical applications.
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http://dx.doi.org/10.1002/marc.202000749DOI Listing
July 2021

RANKL from bone marrow adipose lineage cells promotes osteoclast formation and bone loss.

EMBO Rep 2021 Jul 13;22(7):e52481. Epub 2021 Jun 13.

Institute of Translational Medicine, Shanghai University, Shanghai, China.

Receptor activator of NF-κB ligand (RANKL) is essential for osteoclast formation and bone remodeling. Nevertheless, the cellular source of RANKL for osteoclastogenesis has not been fully uncovered. Different from peripheral adipose tissue, bone marrow (BM) adipose lineage cells originate from bone marrow mesenchymal stromal cells (BMSCs). Here, we demonstrate that adiponectin promoter-driven Cre expression (Adipoq ) can target bone marrow adipose lineage cells. We cross the Adipoq mice with rankl mice to conditionally delete RANKL from BM adipose lineage cells. Conditional deletion of RANKL increases cancellous bone mass of long bones in mice by reducing the formation of trabecular osteoclasts and inhibiting bone resorption but does not affect cortical bone thickness or resorption of calcified cartilage. Adipoq ; rankl mice exhibit resistance to estrogen deficiency and rosiglitazone (ROS)-induced trabecular bone loss but show bone loss induced by unloading. BM adipose lineage cells therefore represent an essential source of RANKL for the formation of trabecula osteoclasts and resorption of cancellous bone during remodeling under physiological and pathological conditions. Targeting bone marrow adiposity is a promising way of preventing pathological bone loss.
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http://dx.doi.org/10.15252/embr.202152481DOI Listing
July 2021

Changes of serum IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 in COVID-19 patients.

Int J Clin Pract 2021 Jun 9:e14462. Epub 2021 Jun 9.

Medical Laboratory and Pathology Center, The First Hospital of Hunan University of Chinese Medicine, Changsha, China.

Background And Purpose: Studies have shown that some cytokines in COVID-19 patients were elevated. This study aims to assess whether IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 serve as potential diagnostic biomarkers of COVID-19.

Methods: The above serum cytokines in COVID-19 patients and non-COVID-19 patients were detected by ELISA and SARS-CoV-2 IgM and IgG were detected by the chemiluminescence method. The independent-sample Mann-Whitney U test was utilised to compare cytokine levels in different groups and courses, the Levene T-test and T'-test were utilised to compare they in different genders and the Spearman correlation test was utilised to analyse the correlation between the cytokine levels with ages and SARS-CoV-2 IgG and IgM.

Results: Serum levels of IL-10, IL-1β, MCP-1, TNF-α and IL-4 in COVID-19 patients were significantly higher than those in non-COVID-19 patients, while IL-6 were only significantly higher than in healthy people, IP-10 were significantly lower than in other diseases patients. AUCs of COVID-19 diagnosed by IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 were 0.735, 0.775, 0.595, 0.821, 0.848, 0.38 and 0.682, respectively. In the COVID-19 patients' serum, the levels of IL-10 and MCP-1 of male were noticeably higher than those of female, and all cytokines were significantly positively correlated with age, IL-1β and IL-4 were significantly negatively correlated with SARS-CoV-2 IgM, while IL-10, IL-1β, IL-6, TNF- and IP-10 were significantly negatively correlated with SARS-CoV-2 IgG. IL-10 on 43-56 days was significantly lower than at 29-42 days, TNF-α at 15-42 days was significantly higher than at 0-14 days, IP-10 at 0-14 days was the highest and IL-4 at 29-42 days was significantly higher than at 0-14 days.

Conclusions: The detection of IL-10, IL-1 β, IL-6, MCP-1, TNF-α and IL-4 would assist the clinical study of COVID-19, and IP-10 may be the cytokine of early elevation in COVID-19 patients.
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http://dx.doi.org/10.1111/ijcp.14462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237069PMC
June 2021

A new trinuclear cluster-based Cd(II) compound: photocatalytic property and nursing application values on the bacterial infection.

Authors:
Yan Hu Jiao Ni

Des Monomers Polym 2021 May 31;24(1):173-181. Epub 2021 May 31.

Xiaoshan District Chengxiang Street Community Health Service Center, Hangzhou, Zhejiang, China.

A new Cd(II) coordination polymer with the formula of {[(CH)NH][Cd(NH-bdc)(btz)(HO)]} ( NH-Hbdc = 2-aminoterephthalic acid, Hbtz = 1 H-benzotriazole) was produced and then it was structurally characterized through powder X-ray diffraction (PXRD), the analysis of X-ray single-crystal diffraction, along with elemental analysis (EA). The photocatalytic property investigations indicate that compound exhibits good activity for photodegradation of methyl violet (MV) with 60.7% of MV removal in 40 min under room temperature. Furthermore, the assessment of the compound's treatment activity and nursing application values on the bacterial infection was conducted and its corresponding mechanism was also studied. Evaluation of the in vitro hemolysis of the compound was determined by measuring the degree of red blood cell lysis and hemoglobin release. The effect of new compounds on the relative proliferation rate of L-929 cells was measured by MTT assay. The ELISA detection kit showed that the compound could reduce the TNF-α and IL-1β content released into plasma. Next, the inhibitory activity of the compound on the bacterial survival gene expression was also proved with real-time RT-PCR. The hemolysis rate of the new compound to blood is 3.4%, which is less than the standard 5%, which is non-hemolytic reaction. The compound also has no obvious cytotoxicity and has good cell compatibility.
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http://dx.doi.org/10.1080/15685551.2021.1935010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168771PMC
May 2021

Histone demethylase PHF8 promotes cell growth and metastasis of non-small-cell lung cancer through activating Wnt/β-catenin signaling pathway.

Histol Histopathol 2021 Jun 8:18349. Epub 2021 Jun 8.

Department of Respiratory, The First People's Hospital of Zigong City, Zigong, China.

PHD finger protein 8 (PHF8), serving as a histone demethylase, is upregulated in some types of malignant tumors. The role of PHF8 in non-small-cancer lung carcinoma (NSCLC) remains unclear. This study aims to verify the effect of PHF8 in NSCLC and its molecular mechanism. We collected 20 cases of fresh NSCLC and adjacent lung tissues to assess differential expressions of PHF8 by reverse transcription-quantitative PCR (RT-qPCR). Western blot was employed to examine protein levels of PHF8, Wnt1, β-catenin and epithelial-mesenchymal transition (EMT) related proteins. Chromatin immunoprecipitation assays were executed to confirm the regulatory mechanism of PHF8 and Wnt1. Cell Counting Kit-8 assays and Transwell assays were utilized to identify the effects of PHF8/Wnt1 pathway on cell proliferation, migration and invasion. PHF8 was overexpressed in NSCLC tissues and cells and higher PHF8 expression was correlated with poorer overall survival in NSCLC patients. PHF8 overexpression promoted NSCLC cell proliferation, migration and invasion, while PHF8 knockdown exerted the opposite effect. Mechanistic investigations identified that PHF8 occupied the Wnt1 promoter, leading to a decrease of repressive histone markers H3K9me1, H3K9me2, H3K27me2 and H4K20me1 in the promoter region of the Wnt1 gene, which further promoted the transcription of the Wnt1 gene. PHF8 activated Wnt/β-catenin signaling pathway through promoting Wnt1 expression. Besides, PHF8 altered the EMT of NSCLC through regulating Wnt1 levels. PHF8, acting as an oncogene and prognostic biomarker in NSCLC, stimulated NSCLC to proliferate, metastasis and EMT by activating Wnt/β-catenin signaling.
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http://dx.doi.org/10.14670/HH-18-349DOI Listing
June 2021

Evolving symptom networks in relation to HIV-positive duration among people living with HIV: A network analysis.

Int J Infect Dis 2021 Jul 8;108:503-509. Epub 2021 Jun 8.

NYU Rory Meyers College of Nursing, New York City, NY, USA.

Objectives: To explore and visualize the relationships among multiple symptoms in people living with HIV (PLWH) and compare centrality indices and the density of symptom networks among groups of individuals with different HIV-positive durations.

Methods: We conducted a secondary analysis of data from the HIV-related Symptoms Monitoring Survey conducted in China. Networks were constructed among 27 symptoms. Centrality properties, including strength and closeness, and network density were used to describe relationships among symptoms in 5 different HIV-positive duration groups.

Results: The findings showed that PLWH with longer HIV-positive durations did not have more severe symptoms; instead, their symptom networks were denser than those of their newly HIV-diagnosed counterparts (F = 27.073, P < 0.001). Fatigue was the most severe and central symptom in PLWH with an HIV-positive duration <10 years (r = 7.79-10.09, r = 18-44, r = 0.01). Confusion was the most central symptom across the 3 centrality indices (r = 11.81, r = 14.00, r = 0.02) in PLWH who had HIV-positive durations >10 years.

Conclusion: This study demonstrates a need to include an assessment of PLWH symptom networks as an essential component of HIV care. We recommended evaluating cognitive function and cognitive training as essential components of HIV care for long-term survivors, even in younger populations (aged ≤50 years).
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http://dx.doi.org/10.1016/j.ijid.2021.05.084DOI Listing
July 2021

IFI35 is involved in the regulation of the radiosensitivity of colorectal cancer cells.

Cancer Cell Int 2021 Jun 3;21(1):290. Epub 2021 Jun 3.

Central Laboratory, The First People's Hospital of Taicang, Taicang Affiliated Hospital of Soochow University, No. 58 Changsheng South Road, Taicang, 215400, Jiangsu, China.

Background: Interferon regulatory factor-1 (IRF1) affects the proliferation of colorectal cancer (CRC). Recombinant interferon inducible protein 35 (IFI35) participates in immune regulation and cell proliferation. The aim of the study was to examine whether IRF1 affects the radiation sensitivity of CRC by regulating IFI35.

Methods: CCL244 and SW480 cells were divided into five groups: blank control, IFI35 upregulation, IFI35 upregulation control, IFI35 downregulation, and IFI35 downregulation control. All groups were treated with X-rays (6 Gy). IFI35 activation by IRF1 was detected by luciferase reporter assay. The GEPIA database was used to examine IRF1 and IFI35 in CRC. The cells were characterized using CCK-8, EdU, cell cycle, clone formation, flow cytometry, reactive oxygen species (ROS), and mitochondrial membrane potential. Nude mouse animal models were used to detect the effect of IFI35 on CRC.

Results: IRF1 can bind to the IFI35 promoter and promote the expression of IFI35. The expression consistency of IRF1 and IFI35 in CRC, according to GEPIA (R = 0.68, p < 0.0001). After irradiation, the upregulation of IFI35 inhibited cell proliferation and colony formation and promoted apoptosis and ROS, while IFI35 downregulation promoted proliferation and colony formation and reduced apoptosis, ROS, and mitochondrial membrane potential were also reduced. The in vivo experiments supported the in vitro ones, with smaller tumors and fewer liver metastases with IFI35 upregulation.

Conclusions: IRF1 can promote IFI35 expression in CRC cells. IFI35 is involved in the regulation of radiosensitivity of CRC cells and might be a target for CRC radiosensitization.
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http://dx.doi.org/10.1186/s12935-021-01997-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176734PMC
June 2021

Corrigendum to "Carboxymethyl chitosan microspheres loaded hyaluronic acid/gelatin hydrogels for controlled drug delivery and the treatment of inflammatory bowel disease" [Int. J. Biol. Macromol., 167 (2021) 1598-1612].

Int J Biol Macromol 2021 Aug 31;184:1064. Epub 2021 May 31.

School of Pharmaceutical Science, South-Central University for Nationalities, Wuhan 6430074, China; National Demonstration Center for Experimental Ethnopharmacology Education, South-Central University for Nationalities, Wuhan 430074, China.

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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.083DOI Listing
August 2021

CRKL is a critical target of Hh-GLI2 pathway in lung adenocarcinoma.

J Cell Mol Med 2021 Jun 2. Epub 2021 Jun 2.

Department of Respiratory and Critical Care, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Lung adenocarcinoma (LUAD) is one of the important components of non-small-cell lung cancer (NSCLC) and leads to many deaths every year. During the initiation and progression of the LUAD, the Hh-GLI2 pathway plays critical roles. Several components of this pathway have been shown to be amplified or overexpressed in LUAD, providing this pathway as an attractive target for therapeutics. However, a gap in our understanding of the Hh-GLI2 pathway is the identity of transcriptional targets of GLI2 that drive LUAD tumorigenesis. Here, we show that the oncogene CRKL is a direct target of GLI2. GLI2 turns on CRKL transcription through binding its second intron. Furthermore, CRKL is an essential mediator for GLI2-driven proliferation and migration of LUAD cells. Depletion of CRKL blunts Hh-GLI2 pathway-mediated cell proliferation and invasion. Lastly, we find that CRKL knockout cells are more sensitive to EGFR-TKI and chemotherapeutics. Taken together, our work here identifies a specific target for Hh-related malignancies and provides CRKL as a promising therapeutic target for LUAD.
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http://dx.doi.org/10.1111/jcmm.16592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256351PMC
June 2021

Effects of 2,4-dichlorophenol exposure on zebrafish: Implications for the sex hormone synthesis.

Aquat Toxicol 2021 Jul 16;236:105868. Epub 2021 May 16.

Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, China. Electronic address:

2,4-Dichlorophenol (2,4-DCP), an estrogenic endocrine disruptor, is widely spread in aquatic environments and may interfere with normal physiological functions in fish. However, the influence of this chemical on the synthesis of sex hormones is not well understood. In the present study, zebrafish (Danio rerio) were exposed to 2,4-DCP (80 and 160 μg/L) with or without fadrozole (an aromatase inhibitor which inhibits the synthesis of estradiol) from 20 to 40 days post fertilization. Then, the sex ratio, the content of vitellogenin (VTG) and sex hormones (androstenedione (ASD), estrone (E1), 17β-estradiol (E2), estriol (E3), testosterone (T) and 11-ketotestosterone (11-KT)) were studied. Furthermore, the expression of genes involved in synthesis of sex hormones (cyp19a1a, cyp19a1b, 17β-hsd, 11β-hsd and cyp11b) along with the DNA methylation in cyp19a1a and cyp19a1b promoters was analyzed. The results showed that 2,4-DCP exposure led to female-biased ratio, increased the content of ASD, E2 and VTG, as well as the ratio of E2/11-KT, while decreased the levels of androgens (T and 11-KT). The sex hormonal change can be explained by the significant up-regulation of cyp19a1a, cyp19a1b, 17β-hsd and 11β-hsd genes. In addition, hypomethylation of cyp19a1a promoter was involved in this process. Notably, fadrozole can partly attenuate 2,4-DCP-induced feminization, and recover the levels of ASD, E2 and 11-KT. Thus, these results demonstrate that 2,4-DCP induces feminization in fish by disrupting the synthesis of sex hormones.
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http://dx.doi.org/10.1016/j.aquatox.2021.105868DOI Listing
July 2021

Nanowire-structured FeP-CoP arrays as highly active and stable bifunctional electrocatalyst synergistically promoting high-current overall water splitting.

J Colloid Interface Sci 2021 Oct 18;600:811-819. Epub 2021 May 18.

Guangxi Key Laboratory of Low Carbon Energy Materials, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China. Electronic address:

The design and construction of highly efficient and durable non-noble metal bifunctional catalysts for oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) in alkaline media is essential for developing the hydrogen economy. To achieve this goal, we have developed a bifunctional nanowire-structured FeP-CoP array catalyst on carbon cloth with uniform distribution through in-situ hydrothermal growth and phosphating treatment. The unique nanowire array structure and the strong electronic interaction between FeP and CoP species have been confirmed. Electrochemical studies have found that the designed FeCo-P/CC catalyst appears excellent HER (130 [email protected] mA cm)/OER (270 [email protected] mA cm) activity and stability. Moreover, the bifunctional FeCo-P/CC catalyst is also used in simulated industrial water splitting system, where the pair catalyst requires about 1.95 and 2.14 V to reach 500 and 1000 mA cm, even superior to the control RuO||Pt/C catalyst, showing good industrial application prospects. These excellent electrocatalytic properties are attributed to the synergy between FeP and CoP species as well as the unique microstructure, which can accelerate charge transfer, expose more active sites and enhance electrolyte diffusion and gas emissions.
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http://dx.doi.org/10.1016/j.jcis.2021.05.074DOI Listing
October 2021
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