Publications by authors named "Yan Dong"

1,322 Publications

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An Analysis of Injury Trends and Disease Burden From Three Surveillance Hospitals in Urumqi From 2006 to 2018.

Front Public Health 2022 22;10:915637. Epub 2022 Jul 22.

Post-doctoral Mobile Research Station of Public Health and Preventive Medicine, School of Public Health, Xinjiang Medical University, Urumqi, China.

Objective: To investigate injury trends, injury distribution, and disease burden from three surveillance hospitals in Urumqi from 2006 to 2018.

Method: Injury data from the National Injury Surveillance System (NISS) from three hospitals in Urumqi (2006 to 2018) were collected to analyze changes in the characteristics of outpatient injury cases. Years of potential life lost (YPLL) were calculated to determine the disease burden of the injury cases.

Results: A total of 161,400 injury cases were recorded over 13 years, and the average age of the patient seeking medical attention was 32.4 years old. Male patients outnumbered female patients with a ratio of 1.6:1, but the proportion of female patients was greater after 45 years of age. The highest number of cases occurred in patients 15-29 years of age, accounting for 26.8% of all injury cases. Injury in females occurred most frequently in the home. A total of 41.4% of injury cases occurred while doing housework. The top three causes of injury were falls (49.7%), blunt force of an object, (13.7%), and motor vehicle accidents (MVA) (13.5%). Years of potential life lost from injury accounted for 7.39% of the total YPLL in the three hospitals.

Conclusion: Males should be targeted for injury prevention and intervention in Urumqi. The prevention of falls, blunt force of objects, and MVA should be made a priority. Injury prevention strategies and targeted projects should be developed to reduce the disease burden of injury.
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http://dx.doi.org/10.3389/fpubh.2022.915637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354953PMC
July 2022

Long-term outcome following microwave ablation of lung metastases from colorectal cancer.

Front Oncol 2022 22;12:943715. Epub 2022 Jul 22.

Department of Interventional Therapy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Purpose: To retrospectively evaluate the safety and efficacy of percutaneous computed tomography (CT)-guided microwave ablation (MWA) in colorectal cancer (CRC) lung metastases, and to analyze prognostic factors.

Materials And Methods: Data were collected from 31 patients with CRC lung metastases from May 2013 to September 2017. They had removed the CRC, no extrapulmonary metastases, no more than three metastases in the lung, the maximum diameter of the lesions was ≤3 cm, and all the lung metastases could be completely ablated. The ablation procedures were performed using a KY-2000 microwave multifunctional therapeutic apparatus. Efficacy is assessed two to four weeks after ablation, and follow-up are performed every three months for two years. The primary outcome was overall survival (OS). The secondary outcomes were progression-free survival (PFS), and complications. Cox regression analysis was used for the evaluation of the statistical significance of factors affecting the end result of MWA therapy. The Kaplan-Meier method was used for estimation of survival rates.

Results: A total of 45 metastatic lung lesions from CRC in 31 patients were treated with CT-guided MWA procedures. The median OS was 76 months. The one, two, three, and five-year survival rates were 93.5%, 80.6%, 61.3%, and 51.6%, respectively. Multivariate analysis showed that the primary tumor from the rectum ( = 0.009) and liver metastases at the diagnosis of lung metastases ( = 0.043) were risk factors affecting OS, while PFS was a protective factor. The median PFS was 13 months. The maximum diameter of lung metastases lesions ( = 0.004) was a risk factor. The interval between pulmonary metastases and MWA (=0.031) was the protective factor. Pneumothorax was observed in 13 out of 36 procedures. Four patients developed pneumothorax requiring drainage tube insertion. No patient deaths occurred within 30 days of ablation. Three out of 31 patients (9.67%) were found to have local recurrence of the original lung metastatic ablation foci.

Conclusion: MWA therapy may be safely and effectively used as a therapeutic tool for the treatment of selected CRC pulmonary metastases, and the prognosis is better in patients without liver metastases at the diagnosis of lung metastases.
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http://dx.doi.org/10.3389/fonc.2022.943715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354679PMC
July 2022

Greater bone marrow fat and myosteatosis are associated with lower vBMD but not asymptomatic vertebral fracture.

Eur Radiol 2022 Aug 6. Epub 2022 Aug 6.

Department of Radiology, Beijing Jishuitan Hospital, Beijing, China.

Objectives: Organ fat may affect bone metabolism and be associated with vertebral fracture (VF). This study aimed to explore relationships between VF, adiposity indexes measured by MRI, and volumetric BMD (vBMD) measured by quantitative CT (QCT).

Methods: Four hundred volunteers, ranging in age from 22 to 83 years, were recruited and underwent same-day abdominal QCT and chemical shift-encoded (CSE) MRI. We used MRI to quantify the fat content of bone marrow (BMF), psoas major and paraspinal muscles, and the liver. Abdominal fat, VF, and vBMD of the lumbar spine were measured by QCT. For VF discrimination analysis, we examined both the whole cohort (60 VF cases in 30 men and 30 women) and a restricted subgroup of those aged over 50 years (50 VF cases in 23 men and 27 women).

Results: Amongst the men, a 1 SD increase in BMF was associated with a 27.67 (95% CI, -32.71 to -22.62) mg/cm decrease in vBMD after adjusting for age and BMI. Amongst women, all adiposity indexes except for liver fat were significantly associated with vBMD, with BMF having the strongest association (β, -24.00; 95% CI, -28.54 to -19.46 mg/cm). Similar findings were also observed in participants aged over 50 years. The associations of adiposity indexes with vertebral fracture were not significant after adjusting for age in both sexes aged over 50 years.

Conclusions: In both sexes, higher bone marrow fat was associated with lower vBMD at the spine. However, marrow fat and other adipose tissues were not associated with radiographic-based prevalent vertebral fractures.

Key Points: • In both sexes, higher bone marrow fat was associated with lower vBMD at the spine. • Among women, all adiposity indexes except for liver fat content were significantly associated with vBMD, with bone marrow fat having the strongest association. • Marrow fat and other adipose tissues were not associated with radiographic-based asymptomatic vertebral fractures.
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http://dx.doi.org/10.1007/s00330-022-08979-xDOI Listing
August 2022

The complete chloroplast genome of (DC.) Norl. (Asteraceae: Asterodae: Calenduleae), an ornamental plant.

Mitochondrial DNA B Resour 2022 28;7(7):1377-1379. Epub 2022 Jul 28.

School of Horticulture, Xinyang Agriculture and Forestry University, Xinyang, Henan, PR China.

(DC.) Norl. 1838 is a herbaceous and perennial plant native to South Africa. It is an ornamental plant that shows great commercial potential. In the present study, the complete chloroplast (cp) genome was 151,295 bp in total length, and 127 genes were identified, including 85 protein-coding, 34 tRNA, and eight rRNA genes. The cp genome includes a large single-copy (LSC) region of 83,293 bp, a small single-copy (SSC) region of 18,012 bp, and a pair of inverted repeats (IRs) regions of 24,995 bp. The phylogenetic relationship of revealed by cp genome provides a foundation for future studies of the phylogeny in the Asteraceae.
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http://dx.doi.org/10.1080/23802359.2022.2101394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341342PMC
July 2022

Tailoring the Boron Configurations in B-doped Na3V2(PO4)[email protected] for Fast and Durable Sodium Storage.

ChemSusChem 2022 Aug 2. Epub 2022 Aug 2.

University of Electronic Science and Technology of China, State Key Laboratory of Electronic Thin Films and Integrated Devices, Jian She Bei Lu, 610054, Chengdu, CHINA.

Na 3 V 2 (PO 4 ) 3 (NVP) is a widely studied cathode material for sodium-ion batteries because of its high ionic conductivity and attractive charge/discharge plateau (3.4 V vs. Na/Na + ). However, its poor electronic conductivity and severe volume expansion during sodium storage need to be addressed before its intensive application could be realized. Herein, we synthesize boron-doped NVP through a facile electrospinning method. By adding boric acid into the reaction mixture during electrospinning followed by carbonization, boron could be directly inserted into the carbon matrix, giving rise to B-doped carbon nanofiber wrapped NVP. By tuning the doping amount, the boron-containing configurations can be facilely manipulated which play different roles in promoting the sodium storage properties of the composite. Based on the calculation results, BC 2 O enhances sodium diffusion by lowering the energy barrier, while BCO 2 improves the structural stability. Due to these specific functionalities of the configurations, the as-prepared composite with a balanced amount of BC 2 O and BCO 2 demonstrates superior sodium storage capacity of 113 mA h g -1 at 1 C, outstanding long cycling performance of 103 mA h g -1 at 10 C and retains 91 mA h g -1 after 1500 cycles. This gives rise to a capacity loss of only 0.08‰ per cycle, much better than the undoped counterpart.
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http://dx.doi.org/10.1002/cssc.202201121DOI Listing
August 2022

Prevalence and heart rate variability characteristics of premature ventricular contractions detected by 24-hour Holter among outpatients with palpitations in China: a cross-sectional study.

BMJ Open 2022 Aug 2;12(8):e059337. Epub 2022 Aug 2.

Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

Objective: To analyse the prevalence and heart rate variability (HRV) characteristics of premature ventricular contraction (PVC) detected by 24-hour Holter among Chinese outpatients with palpitations.

Design: A cross-sectional study.

Setting: This study was conducted in a tertiary hospital.

Participants: A total of 4754 outpatients who received 24-hour Holter for palpitations.

Main Outcome Measures: Prevalence, HRV time-domain and frequency-domain analyses of 24-hour Holter, and echocardiographic parameters were assessed. Propensity score matching (PSM) was applied to balance baseline variables (age, gender) to decrease the bias between comparison groups.

Results: The prevalence of PVC was 67.7% (3220/4754), and was higher in men than women (69.9% vs 66.0%, p=0.004); the prevalence of frequent PVCs (PVC burden≥5%) was 7.7% (368/4754). Older patients had the highest frequency of PVC among all patients. However, among 3220 patients with PVC, younger patients' PVC burden was much higher. Matched 1:1 by age and gender, the HRV time-domain parameters in patients with PVC were all lower than those in patients without PVC (all p<0.05); for the HRV frequency-domain parameters, the patients with frequent PVCs had a higher low frequency/high frequency (LF/HF) ratio (5.4 vs 2.8, p<0.001) than those with PVC burden less than 5%.

Conclusions: The prevalence of PVC and frequent PVCs were 67.7% and 7.7%, respectively, detected by 24-hour Holter among Chinese outpatients with palpitations. Decreased HRV time-domain parameters suggested the occurrence of PVC, and increased LF/HF ratio represented the imbalance of autonomic nervous system in patients with frequent PVCs. Further studies are needed to understand the HRV indexes in PVC patients.
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http://dx.doi.org/10.1136/bmjopen-2021-059337DOI Listing
August 2022

Incidence and clinical characteristics of fall-related injuries among older inpatients at a tertiary grade a hospital in Shandong province from 2018 to 2020.

BMC Geriatr 2022 08 1;22(1):632. Epub 2022 Aug 1.

Department of Respiratory and Critical Care, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, China.

Background: Falls are an important cause of injury and death of older people. Hence, analyzing the multifactorial risk of falls from past cases to develop multifactorial intervention programs is clinically significant. However, due to the small sample size, there are few studies on fall risk analysis of clinical characteristics of fallers, especially among older hospitalized patients.

Methods: We collected data on 153 inpatients who fell (age ≥ 60 years) from the hospital nursing adverse event reporting system during hospitalization at Shandong Provincial Hospital Affiliated to Shandong First Medical University, China, from January 2018 to December 2020. Patient characteristics at the time of the fall, surrounding environment, primary nurse, and adverse fall events were assessed. The enumeration data were expressed as frequency and percentage, and the chi-squared was performed between recurrent fallers and single fallers, and non-injurious and injurious fall groups.

Results: Cross-sectional data showed 18.3% of the 153 participants experienced an injurious fall. Compared with single fallers, a large proportion of older recurrent fallers more often experienced preexisting conditions such as cerebrovascular disease or taking hypoglycemic drugs. They were exposed to higher risks and could experience at least 3 fall times in 3 months. Besides, the credentials of their responsible nurses were often higher. Factors that increased the risk of a fall-related injury were hypoglycemic drugs (OR 2.751; 95% CI 1.114-6.795), and nursing adverse events (OR 47.571; 95% CI 14.392-157.247). Older inpatients with bed rails (OR 0.437; 95% CI 0.190-1.005) or falling at the edge of the bed (OR 0.365; 95% CI 0.138-0.964) were less likely to be injured than those without bed rails or not falling at the edge of the bed. Fall risks were significantly correlated with more severe fall-related injuries. Older patients with moderate (OR 5.517; CI 0.687-44.306) or high risk (OR 2.196; CI 0.251-19.219) were more likely to experience fall-related injuries than those with low risk.

Conclusions: Older inpatient falls are an ongoing challenge in hospitals in China. Our study found that the incidence of fall-related injuries among inpatients aged ≥ 60 years remained at a minor level. However, complex patient characteristics and circumstances can contribute to fall-related injuries. This study provides new evidence on fall-related injuries of older inpatients in China. Based on the factors found in this study, regular fall-related injury epidemiological surveys that investigate the reasons associated with the injuries were crucial when considering intervention measures that could refine fall-related injuries. More prospective studies should be conducted with improved and updated multidisciplinary fall risk assessment and comprehensive geriatric assessment as part of a fall-related injury prevention protocol.
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http://dx.doi.org/10.1186/s12877-022-03321-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341405PMC
August 2022

LncRNA CRLM1 inhibits apoptosis and promotes metastasis through transcriptional regulation cooperated with hnRNPK in colorectal cancer.

Cell Biosci 2022 Jul 30;12(1):120. Epub 2022 Jul 30.

Department of Oncology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.

Background: Colorectal liver metastases (CRLM) continue to have a low survival rate. The number of CRLM regulators and clinical indicators remains limited. Long non-coding RNAs (lncRNAs) are a new master regulator of cell invasion and metastasis. However, the function and regulation mechanism of lncRNAs in colorectal cancer (CRC) metastasis are yet unknown.

Methods: To screen and identify CRLM-related lncRNAs, public transcriptome data were used. Gain and loss of function experiments were carried out to investigate the biological activities of lncRNA CRLM1 in vitro and in vivo. RNA sequencing (RNA-seq), chromatin isolation by RNA purification (ChIRP), immunofluorescence (IF), quantitative real-time PCR (qRT-PCR), western blotting, and rescue experiments were performed to explore the molecular mechanism of CRLM1. Moreover, identified the proteins, DNAs, and RNAs that interact with CRLM1.

Results: The investigation of lncRNA expression dynamics in CRLM, primary CRC, and normal tissues in this work resulted in identifying a series of lncRNAs associated with metastasis, including CRLM1. CRLM1 inhibited apoptosis of CRC cells and promoted liver metastasis in Balb/C nude mice. CRLM1 was weakly associated with the chromatin regions of genes involved in cell adhesion and DNA damage, and this association was bidirectionally correlated with CRLM1-regulated pro-metastatic gene expression. CRLM1 physically interacts with the hnRNPK protein and promotes its nuclear localization. CRLM1 effectively enhances hnRNPK promoter occupancy and co-regulates the expression of a panel of metastatic genes.

Conclusions: The finding of the clinically significant lncRNA CRLM1 in promoting metastasis and regulating gene expression suggests a potential biomarker and target for CRLM therapy.
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http://dx.doi.org/10.1186/s13578-022-00849-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338583PMC
July 2022

Stable, Bright, and Long-Fluorescence-Lifetime Dyes for Deep-Near-Infrared Bioimaging.

J Am Chem Soc 2022 Jul 29. Epub 2022 Jul 29.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

Near-infrared (NIR) fluorophores absorbing maximally in the region beyond 800 nm, i.e., deep-NIR spectral region, are actively sought for biomedical applications. Ideal dyes are bright, nontoxic, photostable, biocompatible, and easily derivatized to introduce functionalities (e.g., for bioconjugation or aqueous solubility). The rational design of such fluorophores remains a major challenge. Silicon-substituted rhodamines have been successful for bioimaging applications in the red spectral region. The longer-wavelength silicon-substituted congeners for the deep-NIR spectral region are unknown to date. We successfully prepared four silicon-substituted bis-benzannulated rhodamine dyes (), with an efficient five-step cascade on a gram-scale. Because of the extensive overlapping of their HOMO-LUMO orbitals, are highly absorbing (λ ≈ 865 nm and ε > 10 cm M). By restraining both the rotational freedom via annulation and the vibrational freedom via silicon-imparted strain, the fluorochromic scaffold of is highly rigid, resulting in an unusually long fluorescence lifetime (τ > 700 ps in CHCl) and a high fluorescence quantum yield (ϕ = 0.14 in CHCl). Their half-lives toward photobleaching are 2 orders of magnitude longer than the current standard (ICG in serum). They are stable in the presence of biorelevant concentration of nucleophiles or reactive oxygen species. They are minimally toxic and readily metabolized. Upon tail vein injection of (as an example), the vasculature of a nude mouse was imaged with a high signal-to-background ratio. dyes have broad potentials for bioimaging in the deep-NIR spectral region.
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http://dx.doi.org/10.1021/jacs.2c05826DOI Listing
July 2022

Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL.

Theor Appl Genet 2022 Jul 29. Epub 2022 Jul 29.

Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.

Key Message: We fine mapped QTL QTKW.caas-5DL for thousand kernel weight in wheat, predicted candidate genes and developed a breeding-applicable marker. Thousand kernel weight (TKW) is an important yield component trait in wheat, and identification of the underlying genetic loci is helpful for yield improvement. We previously identified a stable quantitative trait locus (QTL) QTKW.caas-5DL for TKW in a Doumai/Shi4185 recombinant inbred line (RIL) population. Here we performed fine mapping of QTKW.caas-5DL using secondary populations derived from 15 heterozygous recombinants and delimited the QTL to an approximate 3.9 Mb physical interval from 409.9 to 413.8 Mb according to the Chinese Spring (CS) reference genome. Analysis of genomic synteny showed that annotated genes in the physical interval had high collinearity among CS and eight other wheat genomes. Seven genes with sequence variation and/or differential expression between parents were predicted as candidates for QTKW.caas-5DL based on whole-genome resequencing and transcriptome assays. A kompetitive allele-specific PCR (KASP) marker for QTKW.caas-5DL was developed, and genotyping confirmed a significant association with TKW but not with other yield component traits in a panel of elite wheat cultivars. The superior allele of QTKW.caas-5DL was frequent in a panel of cultivars, suggesting that it had undergone positive selection. These findings not only lay a foundation for map-based cloning of QTKW.caas-5DL but also provide an efficient tool for marker-assisted selection.
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http://dx.doi.org/10.1007/s00122-022-04182-0DOI Listing
July 2022

Identification of fatty acid metabolism-related lncRNAs in the prognosis and immune microenvironment of colon adenocarcinoma.

Biol Direct 2022 Jul 28;17(1):19. Epub 2022 Jul 28.

Department of Oncology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.

Background: Cancer metabolism is largely altered compared to normal cells. This study aims to explore critical metabolism pathways in colon adenocarcinoma (COAD), and reveal the possible mechanism of their role in cancer progression.

Methods: Expression data and sequencing data of COAD samples were obtained from The Cancer Genome Atlas and Gene Expression Omnibus databases. The expression profiles between tumor and normal samples were compared to identify differential metabolism pathways through single sample gene set enrichment analysis.

Results: Fatty acid synthesis was identified as a key metabolism pathway in COAD. Based on fatty acid-related lncRNAs, two molecular subtypes (C1 and C2) were defined. C2 subtype with worse prognosis had higher immune infiltration and higher expression of immune checkpoints. Five transcription factors (TFs) including FOS, JUN, HIF1A, STAT3 and STAT2 were highly expressed in C2 subtype. Five fatty acid-related lncRNAs were identified to be biomarkers for predicting COAD prognosis. Finally, further experients showed that knockdown of lncRNA PAXIP1-AS1 decreased the triglyceride content and the fatty acid synthase and acetyl-CoA carboxylase 1 expressions, which suggested that lncRNA PAXIP1-AS1 plays an important role in fatty acid metabolism of COAD.

Conclusions: This study demonstrated that fatty acid synthesis was greatly altered in COAD. Fatty acid-related lncRNAs were speculated to be involved in cancer progression through associating with TFs. The five screened TFs may serve as new drug targets for treating COAD.
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http://dx.doi.org/10.1186/s13062-022-00332-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331591PMC
July 2022

Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat.

Theor Appl Genet 2022 Jul 26. Epub 2022 Jul 26.

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

Key Message: Twelve QTL associated with pre-harvest sprouting tolerance were identified using association analysis in wheat. Two markers were validated and a candidate gene TaNAC074 for Qgpf.cas-3B.2 was verified using Agrobacterium-mediated transformation. Pre-harvest sprouting (PHS) is a considerable global threat to wheat yield and quality. Due to this threat, breeders must identify quantitative trait loci (QTL) and genes conferring PHS-tolerance (PHST) to reduce the negative effects of PHS caused by low seed dormancy. In this study, we evaluated a panel of 302 diverse wheat genotypes for PHST in four environments and genotyped the panel with a high-density wheat 660 K SNP array. By using a genome-wide association study (GWAS), we identified 12 stable loci significantly associated with PHST (P < 0.0001), explaining 3.34 - 9.88% of the phenotypic variances. Seven of these loci co-located with QTL and genes reported previously. Five loci (Qgpf.cas-3B.2, Qgpf.cas-3B.3, Qgpf.cas-3B.4, Qgpf.cas-7B.2, and Qgpf.cas-7B.3), located in genomic regions with no known PHST QTL or genes, are likely to be new QTL conferring PHST. Additionally, two molecular markers were developed for Qgpf.cas-3A and Qgpf.cas-7B.3, and validated using a different set of 233 wheat accessions. Finally, the PHST-related function of candidate gene TaNAC074 for Qgpf.cas-3B.2 was confirmed by CAPS (cleaved amplified polymorphic sequences) marker association analysis in 233 wheat accessions and by expression and phenotypic analysis of transgenic wheat. Overexpression of TaNAC074 significantly reduced seed dormancy in wheat. This study contributes to broaden the genetic basis and molecular marker-assisted breeding of PHST.
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http://dx.doi.org/10.1007/s00122-022-04184-yDOI Listing
July 2022

Using 16S rDNA Sequencing Technology to Preliminarily Analyze Intestinal Flora in Children with Pneumonia.

Biomed Environ Sci 2022 Jun;35(6):528-537

Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.

Objective: We investigated changes in the intestinal flora of children with pneumonia (MPP).

Methods: Between September 2019 and November 2019, stool samples from 14 children with MPP from The Fourth Hospital of Baotou city, Inner Mongolia Autonomous Region, were collected and divided into general treatment (AF) and probiotic (AFY) groups, according to the treatment of "combined and tablets live". High-throughput sequencing was used to identify intestinal flora.

Results: Intestinal flora abundance and diversity in children with MPP were decreased. Both Shannon and Simpson indices were lower in the AF group when compared with healthy controls ( < 0.05). When compared with healthy controls, the proportion of was lower in the AF group, while the proportion of was higher ( < 0.05). The proportion of and was lower in the AFY group but , , and proportions were higher. The proportion of - in the AFY group after treatment was decreased ( < 0.05).

Conclusions: The intestinal flora of children with MPP is disturbed, manifested as decreased abundance and diversity, and decreased . Our probiotic mixture partly improved intestinal flora disorders.
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http://dx.doi.org/10.3967/bes2022.070DOI Listing
June 2022

Regulatory effect of gut microbes on blood pressure.

Animal Model Exp Med 2022 Jul 26. Epub 2022 Jul 26.

Xinxiang Key Laboratory of Pathogenic Biology, Department of Pathogenic Biology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, China.

Hypertension is an important global public health issue because of its high morbidity as well as the increased risk of other diseases. Recent studies have indicated that the development of hypertension is related to the dysbiosis of the gut microbiota in both animals and humans. In this review, we outline the interaction between gut microbiota and hypertension, including gut microbial changes in hypertension, the effect of microbial dysbiosis on blood pressure (BP), indicators of gut microbial dysbiosis in hypertension, and the microbial genera that affect BP at the taxonomic level. For example, increases in Lactobacillus, Roseburia, Coprococcus, Akkermansia, and Bifidobacterium are associated with reduced BP, while increases in Streptococcus, Blautia, and Prevotella are associated with elevated BP. Furthermore, we describe the potential mechanisms involved in the regulation between gut microbiota and hypertension. Finally, we summarize the commonly used treatments of hypertension that are based on gut microbes, including fecal microbiota transfer, probiotics and prebiotics, antibiotics, and dietary supplements. This review aims to find novel potential genera for improving hypertension and give a direction for future studies on gut microbiota in hypertension.
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http://dx.doi.org/10.1002/ame2.12233DOI Listing
July 2022

Molten Salt Synthesis of Broad-Band Near-Infrared InBO:Cr Submicron Phosphor and Its Luminescent Enhancement by Lanthanide Ion Codoping.

Inorg Chem 2022 Aug 25;61(31):12275-12283. Epub 2022 Jul 25.

School of Mechanical and Electronic Engineering, Nanjing Forestry University, Nanjing 210037, P. R. China.

Phosphor materials with small particle sizes and high luminescent efficiency are desired for the fabrication of phosphor-converted light-emitting diodes (pc-LEDs). Near-infrared (NIR) pc-LED light sources have great application potential in the food industry and medical fields, which stimulate the extensive exploration of NIR phosphors. In this work, broad-band NIR-emitting InBO:Cr phosphors with submicron size and spherical morphology are successfully synthesized via the molten salt method. The InBO:Cr phosphor exhibits a broad emission band covering 700-1000 nm and peaking at ∼820 nm. The maximum emission intensity is obtained for InBO:0.02Cr with an internal quantum yield (IQY) of ∼62%, which is higher than that of microsized counterparts derived from solid-state reaction. Furthermore, the absorption and emission enhancements are achieved by codoping lanthanide ions into InBO:Cr submicron phosphors. The codoping of inert La ions can increase the absorption efficiency of InBO:Cr, due to the increased octahedral distortion of Cr sites. The codoping of active Yb ions can significantly enhance the NIR emissions of InBO:Cr between 950 and 1100 nm. Meanwhile, the increased IQY of ∼73% is achieved for InBO:0.02Cr,0.005Yb simultaneously with suppressed thermal quenching, originating from the effective energy transfer from Cr to Yb ions.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01477DOI Listing
August 2022

Sensing gastric cancer exosomes with MoS-based SERS aptasensor.

Biosens Bioelectron 2022 Jul 9;215:114553. Epub 2022 Jul 9.

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China.

Exosomes have been widely used in early cancer diagnosis as promising cancer biomarkers due to their abundant tumor-specific molecular information. In this study, we developed a sensitive and straightforward surface-enhanced Raman scattering (SERS) aptasensor to detect exosomes based on gold nanostars-decorated molybdenum disulfide (MoS) nanocomposites (MoS-AuNSs). ROX-labeled aptamers (ROX-Apt) were assembled on MoS-AuNSs surface as recognition probes that specifically bind with transmembrane protein CD63 (a representative surface marker on exosomes). Thus obvious ROX Raman signals were obtained through the synergistic Raman enhancement effect of AuNSs and MoS nanosheet. In presence of exosomes, ROX-Apt is preferentially tethered onto exosomes and released from the surface of nanocomposites, resulting in a decrease of the SERS signal. Expectedly, the as-fabricated SERS aptasensor was capable of detecting exosomes in a wide range from 55 to 5.5 × 10 particles μL with a detection limit of 17 particles μL. Moreover, the aptasensor exhibited accepted stability and potential clinical applicability.
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http://dx.doi.org/10.1016/j.bios.2022.114553DOI Listing
July 2022

Battery-free, tuning circuit-inspired wireless sensor systems for detection of multiple biomarkers in bodily fluids.

Sci Adv 2022 Jul 6;8(27):eabo7049. Epub 2022 Jul 6.

Department of Materials Science and Engineering, The Ohio State University, Columbus, OH 43220, USA.

Tracking the concentration of biomarkers in biofluids can provide crucial information about health status. However, the complexity and nonideal form factors of conventional digital wireless schemes impose challenges in realizing biointegrated, lightweight, and miniaturized sensors. Inspired by the working principle of tuning circuits in radio frequency electronics, this study reports a class of battery-free wireless biochemical sensors: In a resonance circuit, the coupling between a sensing interface and an inductor-capacitor oscillator through a pair of varactor diodes converts a change in electric potential into a modulation in capacitance, resulting in a quantifiable shift of the resonance circuit. Proper design of sensing interfaces with biorecognition elements enables the detection of various biomarkers, including ions, neurotransmitters, and metabolites. Demonstrations of "smart accessories" and miniaturized probes suggest the broad utility of this circuit model. The design concepts and sensing strategies provide a realistic pathway to building biointegrated electronics for wireless biochemical sensing.
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http://dx.doi.org/10.1126/sciadv.abo7049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258955PMC
July 2022

Longitudinal effect of self-control on reactive-proactive aggression: Mediating roles of hostile rumination and moral disengagement.

Aggress Behav 2022 Jul 19. Epub 2022 Jul 19.

Faculty of Psychology, Southwest University, Chongqing, China.

Self-control is a well-known inhibitor of aggression, but the effect of self-control on different kinds of aggression (such as reactive-proactive aggression) and the underlying mediating mechanisms of these effects are unclear. We developed a mediation model to address these issues. A three-wave study was conducted with a sample of 1203 qualifying Chinese undergraduates to test the model. The results showed that self-control at Wave 1 negatively predicted reactive aggression at Wave 3 through mediating effects of hostile rumination and moral disengagement at Wave 2 at the same time, while self-control at Wave 1 negatively predicted proactive aggression at Wave 3 only through moral disengagement at Wave 2. Furthermore, the longitudinal relationship between hostile rumination and moral disengagement is mutual. The current findings support our hypotheses regarding the mediation model of self-control inhibiting reactive-proactive aggression and suggest that moral disengagement should be a common and basic variable to predict most kinds of aggression; further, hostile rumination only has a particular effect on reactive aggression. The present study used motivation theory to explain its mediation model, which develops aggressive theory regarding varied common influencing factors and underlying mediating mechanisms of reactive and proactive aggression.
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http://dx.doi.org/10.1002/ab.22046DOI Listing
July 2022

CRISPR-empowered hybridization chain reaction amplification for an attomolar electrochemical sensor.

Chem Commun (Camb) 2022 Aug 4;58(63):8826-8829. Epub 2022 Aug 4.

School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

Rapid pathogen screening holds the key against certain viral infections, especially in an overwhelming pandemic. Herein, a CRISPR-empowered electrochemical biosensor was designed for the ultrasensitive detection of the avian influenza A (H7N9) virus gene sequence. Combining the CRISPR/Cas system, a signal-amplification strategy and a high-conductivity sensing substrate, the developed biosensor showed an ultrawide dynamic range, an ultralow detection limit, and excellent selectivity for H7N9 detection, providing a potential sensing platform for the simple, fast, sensitive, and on-site detection of infectious diseases.
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http://dx.doi.org/10.1039/d2cc01155gDOI Listing
August 2022

Contingent Amygdala Inputs Trigger Heterosynaptic LTP at Hippocampus-to-Accumbens Synapses.

J Neurosci 2022 Jul 15. Epub 2022 Jul 15.

Department of Neuroscience, University of Pittsburgh, Pittsburgh, PA 15260

The nucleus accumbens shell (NAcSh) is a key brain region where environmental cues acquire incentive salience to reinforce motivated behaviors. Principal medium spiny neurons (MSNs) in the NAcSh receive extensive glutamatergic projections from limbic regions, among which, the ventral hippocampus (vH) transmits information enriched in contextual cues, and the basolateral amygdala (BLA) encodes real-time arousing states. The vH and BLA project convergently to NAcSh MSNs, both activated in a time-locked manner upon a cue-conditioned motivational action. In brain slices prepared from male and female mice, we show that co-activation of the two projections induces long-term potentiation (LTP) at vH-to-NAcSh synapses without affecting BLA-to-NAcSh synapses, revealing a heterosynaptic mechanism through which BLA signals persistently increase the temporally contingent vH-to-NAcSh transmission. Furthermore, this LTP is more prominent in dopamine D1 receptor-expressing (D1) MSNs than D2 MSNs and can be prevented by inhibition of either D1 receptors or dopaminergic terminals in NAcSh. This heterosynaptic LTP may provide a dopamine-guided mechanism through which vH-encoded cue inputs that are contingent to BLA activation acquire increased circuit representation to reinforce behavior.In motivated behaviors, environmental cues associated with arousing stimuli acquire increased incentive salience, processes mediated in part by the nucleus accumbens (NAc). NAc principal neurons receive glutamatergic projections from the ventral hippocampus (vH) and basolateral amygdala, which transmit information encoding contextual cues and affective states, respectively. Our results show that co-activation of the two projections induces long-term potentiation (LTP) at vH-to-NAc synapses without affecting BLA-to-NAc synapses, revealing a heterosynaptic mechanism through which BLA signals potentiate the temporally contingent vH-to-NAc transmission. Furthermore, this LTP is prevented by inhibition of either D1 receptors or dopaminergic axons. This heterosynaptic LTP may provide a dopamine-guided mechanism through which vH-encoded cue inputs that are contingent to BLA activation acquire increased circuit representation to reinforce behavior.
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http://dx.doi.org/10.1523/JNEUROSCI.0838-22.2022DOI Listing
July 2022

Fretting stimulation enhances bone growth at the interface between hydroxyapatite coating and bone.

Colloids Surf B Biointerfaces 2022 Jul 5;217:112669. Epub 2022 Jul 5.

School of Materials and Physics, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China. Electronic address:

Biologically fixed arthroplasty is limited in its development by the long postoperative recovery time and the low quality of solidity of the fixed interface in the short postoperative period. Therefore, fretting stimulation is used to accelerate the combination between bone tissue and the biological fixation interface of artificial joint prostheses. The effects of different compression loads and tangential micro-motion amplitude on the growth rate of bone tissue and the firm quality of fixation interface were studied by using two kinds of micro-motion stimuli: compression and tangential micro-motion. The mechanism of micro-motion stimulation to promote bone growth at the fixation interface was revealed. The results of binding force detection of biological fixation interface and bone tissue section staining showed that the bone tissue and hydroxyapatite coating interface had the most tendency to produce new bone tissue under compression load of 4 N. In the tangential fretting environment, the tangential fretting amplitude of ± 40 µm and the normal load of 7.5 N were the most conducive to bone growth, making the combination of bone tissue and titanium alloy prosthesis coated with hydroxyapatite more firm. The study is important for accelerating the integration and shortening the rehabilitation time after artificial joint replacement.
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http://dx.doi.org/10.1016/j.colsurfb.2022.112669DOI Listing
July 2022

Effect of different amounts of bamboo charcoal on properties of biodegradable bamboo charcoal/polylactic acid composites.

Int J Biol Macromol 2022 Jul 7;216:456-464. Epub 2022 Jul 7.

College of Materials Science and Engineering, Central South University of Forestry and Technology, 410004 Changsha, PR China. Electronic address:

Biodegradable composites were prepared from polylactic acid (PLA) and bamboo charcoal (BC) by melt blending and hot pressing. The effects of BC addition on the mechanical properties, water absorption, DMA, TGA, DSC, and CONE of BC/PLA composites were investigated. The microscopic morphology of the composites was analyzed by SEM. The results showed that for BC addition of 40 wt%, the mechanical strength, thermal properties, and flame retardant properties of the composites were improved compared with those of PLA, with a 2.24 % increase in flexural strength and a 1535 % increase (500 °C) in TG mass retention rate. The crystallinity increased by 129.66 %, the peak loss factor decreased by 31.15 %, the time required for combustion was delayed by 168 s, the peak heat release rate decreased by 29.40 %, the carbon residue rate detected by cone calorimetry increased by 48.50 %, and the peak mass loss rate decreased by 48.82 %. The addition of BC enhanced the crystallization capacity of PLA, and improved the thermal properties and flame retardant properties of the prepared composites. The results showed that materials prepared with a BC content of 40 wt% exhibited the best overall performance.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.06.209DOI Listing
July 2022

Conjunctive Analyses of BSA-Seq and BSR-Seq Unveil the and as Key Candidate Genes for Cytoplasmic Male Sterility in Alfalfa ( L.).

Int J Mol Sci 2022 Jun 28;23(13). Epub 2022 Jun 28.

College of Forestry and Grassland, Jilin Agricultural University, Changchun 130118, China.

Knowing the molecular mechanism of male sterility in alfalfa is important to utilize the heterosis more effectively. However, the molecular mechanisms of male sterility in alfalfa are still unclear. In this study, the bulked segregant analysis (BSA) and bulked segregant RNA-seq (BSR) were performed with F2 separation progeny to study the molecular mechanism of male sterility in alfalfa. The BSA-seq analysis was located in a candidate region on chromosome 5 containing 626 candidate genes which were associated with male sterility in alfalfa, while the BSR-seq analysis filtered seven candidate DEGs related to male sterility, and these candidate genes including , , , , and . The conjunctive analyses of BSR and BSA methods revealed that the genes of and are the common detected candidate genes involved in male sterility in alfalfa. Our research provides a theory basis for further study of the molecular mechanism of male sterility in alfalfa and significant information for the genetic breeding of .
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http://dx.doi.org/10.3390/ijms23137172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266382PMC
June 2022

Rational Design Hierarchical SnS Uniformly Adhered to Three-Sided Carbon Active Sites to Enhance Sodium Storage.

ACS Appl Mater Interfaces 2022 Jul 6;14(28):32096-32104. Epub 2022 Jul 6.

College of Chemical and Environmental Engineering, Yancheng Teachers University, Yancheng 224000, P. R. China.

Reducing material accumulation and designing reasonable sizes are critical strategies for increasing the rate and cycling stability of electrode materials. Herein, we presented a double-walled hollow carbon spheres (DWHCSs) loading strategy for achieving ultrafine SnS nanosheet adhesion by utilizing three-sided active sites of the interior/exterior carbon walls. The structure effectively shortened the electron/ion transport path, increased the effective contact between electrolyte and electrode material, and promoted ion diffusion kinetics. Furthermore, the hollow structure can adapt to the volume change of the electrode during the cycle, preventing active substances from draining. Based on the above advantages, [email protected] as an anode material for sodium ion batteries (SIBs) exhibited a distinguished reversible capacity of 665.7 mA h g at 2 A g after 1000 cycles, and a superior rate ability of 377.6 mA h g at an ultrahigh rate of 10 A g. The outstanding electrochemical performance revealed that the structure exhibited a broad application prospect in the field of energy storage and provided a reference for the rational design of other 2D materials.
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http://dx.doi.org/10.1021/acsami.2c08253DOI Listing
July 2022

Saponins Alleviate Coronary Artery Disease Through Hypermethylation of the miR-194-MAPK Pathway.

Front Pharmacol 2022 16;13:829416. Epub 2022 Jun 16.

Department of Cardiology, Guang Anmen Hospital, Beijing, China.

saponins (PNS) may have an inhibitory effect against coronary artery disease (CAD); however, the mechanism is unclear. Recent research has begun to evaluate the role of epigenetics in CAD. Our team found that hypomethylation of miR-194 could be an important mechanism of CAD. The aim of this study was to investigate the effect of PNS against CAD and evaluate whether the mechanism is related to methylation of mi-R194. We conducted a randomized controlled trial with a double-blind placebo design on 84 patients with CAD. Treatment was continued for 4 weeks, and the clinical effect of PNS on CAD was observed. Methylation of miR-194, its promoter, and the key nodes of the MAPK pathway were measured by pyrosequencing and qRT-PCR. We then conducted a pharmacological analysis of the active components of PNS. The effects of PNS on oxidized human umbilical vein endothelial cells and the methylation of miR-194, its promoter, and the key nodes of the MAPK pathway were measured through methylation-specific PCR (MSPCR), qRT-PCR, Western blot analysis, and annexin V/propidium iodide apoptosis assay. PNS improved symptoms of CAD. High-density lipoprotein and white blood cell count demonstrated significant changes after treatment in the PNS group. No significant difference was observed between miR-194 and mRNA MAPK, FAS, RAS, and FOS in the PNS group after treatment. However, some notable trends were observed in these genes. The targets of PNS were predicted by the pharmacological components. Some targets were found to be differentially expressed genes in CAD sequencing. Six genes, including MAPK1, RAS, and FASL, were common targets of PNS in CAD sequencing. Correlations were observed between genes in the interaction network and clinical parameters. experiments confirmed that PNS could change the methylation of miR-194, its promoter, and MAPK, FAS, RAS, and FOS. Intervention with PNS is likely to improve apoptosis. We reported the regulation of miR-194 promoter, miR-194, and MAPK methylation by PNS through cell experiments and a randomized controlled trial. PNS can be used for intervention in CAD by targeting the miR-194 promoter-miR-194-MAPK signaling pathway. : https://www.clinicaltrials.gov/, NCT03083119.
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http://dx.doi.org/10.3389/fphar.2022.829416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243564PMC
June 2022

SMANet: multi-region ensemble of convolutional neural network model for skeletal maturity assessment.

Quant Imaging Med Surg 2022 Jul;12(7):3556-3568

China Academy of Information and Communications Technology, Beijing, China.

Background: Bone age assessment (BAA) is a crucial research topic in pediatric radiology. Interest in the development of automated methods for BAA is increasing. The current BAA algorithms based on deep learning have displayed the following deficiencies: (I) most methods involve end-to-end prediction, lacking integration with clinically interpretable methods; (II) BAA methods exhibit racial and geographical differences.

Methods: A novel, automatic skeletal maturity assessment (SMA) method with clinically interpretable methods was proposed based on a multi-region ensemble of convolutional neural networks (CNNs). This method predicted skeletal maturity scores and thus assessed bone age by utilizing left-hand radiographs and key regional patches of clinical concern.

Results: Experiments included 4,861 left-hand radiographs from the database of Beijing Jishuitan Hospital and revealed that the mean absolute error (MAE) was 31.4±0.19 points (skeletal maturity scores) and 0.45±0.13 years (bone age) for the carpal bones-series and 29.9±0.21 points and 0.43±0.17 years, respectively, for the radius, ulna, and short (RUS) bones series based on the Tanner-Whitehouse 3 (TW3) method.

Conclusions: The proposed automatic SMA method, which was without racial and geographical influence, is a novel, automatic method for assessing childhood bone development by utilizing skeletal maturity. Furthermore, it provides a comparable performance to endocrinologists, with greater stability and efficiency.
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http://dx.doi.org/10.21037/qims-21-1158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246748PMC
July 2022

The Association Between the Occurrence of Common Treatment-Emergent Adverse Events and Efficacy Outcomes After Lasmiditan Treatment of a Single Migraine Attack: Secondary Analyses from Four Pooled Randomized Clinical Trials.

CNS Drugs 2022 Jul 2;36(7):771-783. Epub 2022 Jul 2.

Albert Einstein School of Medicine, Bronx, NY, USA.

Background: In controlled clinical trials, compared with placebo, a significantly greater proportion of participants using lasmiditan to treat a migraine attack achieved 2-h pain freedom (PF) and experienced ≥ 1 treatment-emergent adverse event (TEAE).

Objective: To better inform clinicians about treatment expectations by evaluating the association between TEAEs and efficacy outcomes after lasmiditan treatment.

Methods: Pooled data from SAMURAI, SPARTAN, MONONOFU, and CENTURION were analyzed. A common TEAE (CTEAE) was defined as occurring in ≥ 2% in the overall population. Central nervous system (CNS)-CTEAEs were based on Medical Dictionary for Regulatory Activities.

Results: At 2 h, a significantly higher percentage of lasmiditan 200 mg-treated participants who achieved PF experienced ≥ 1 CTEAE than non-responders who continued to experience moderate/severe pain (48.2% vs. 28.7%, respectively). Correspondingly, a significantly higher percentage of lasmiditan 200 mg-treated participants who experienced ≥ 1 CTEAE achieved PF at 2 h than those who did not (39.0% vs. 30.2%, respectively). Similar results were generally observed with individual CNS-CTEAEs, but for non-CNS-CTEAEs, this pattern was less evident or in the opposite direction. No consistent differences were observed for migraine-related functional disability freedom. The percentage of participants with improved patient global impression of change (PGIC) was greater with a CNS-CTEAE versus no CNS-CTEAE.

Conclusions: Those who had PF at 2 h were more likely to experience a CNS-CTEAE, and those with CNS-CTEAEs were more likely to experience PF. The occurrence of CTEAEs did not seem to negatively affect disability freedom or PGIC.

Clinicaltrials:

Gov Registration: SAMURAI (NCT02439320), SPARTAN (NCT02605174), MONONOFU (NCT03962738), CENTURION (NCT03670810), ClinicalTrials.gov: NCT02439320, NCT02605174, NCT03962738, NCT03670810.
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http://dx.doi.org/10.1007/s40263-022-00928-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259541PMC
July 2022

Natural killer cell homing and trafficking in tissues and tumors: from biology to application.

Signal Transduct Target Ther 2022 06 29;7(1):205. Epub 2022 Jun 29.

Institute of Materia Medica, College of Pharmacy, Army Medical University, 400038, Chongqing, China.

Natural killer (NK) cells, a subgroup of innate lymphoid cells, act as the first line of defense against cancer. Although some evidence shows that NK cells can develop in secondary lymphoid tissues, NK cells develop mainly in the bone marrow (BM) and egress into the blood circulation when they mature. They then migrate to and settle down in peripheral tissues, though some special subsets home back into the BM or secondary lymphoid organs. Owing to its success in allogeneic adoptive transfer for cancer treatment and its "off-the-shelf" potential, NK cell-based immunotherapy is attracting increasing attention in the treatment of various cancers. However, insufficient infiltration of adoptively transferred NK cells limits clinical utility, especially for solid tumors. Expansion of NK cells or engineered chimeric antigen receptor (CAR) NK cells ex vivo prior to adoptive transfer by using various cytokines alters the profiles of chemokine receptors, which affects the infiltration of transferred NK cells into tumor tissue. Several factors control NK cell trafficking and homing, including cell-intrinsic factors (e.g., transcriptional factors), cell-extrinsic factors (e.g., integrins, selectins, chemokines and their corresponding receptors, signals induced by cytokines, sphingosine-1-phosphate (S1P), etc.), and the cellular microenvironment. Here, we summarize the profiles and mechanisms of NK cell homing and trafficking at steady state and during tumor development, aiming to improve NK cell-based cancer immunotherapy.
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http://dx.doi.org/10.1038/s41392-022-01058-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243142PMC
June 2022

Cellular imaging properties of phosphorescent iridium(III) complexes substituted with ester or amide groups.

Dalton Trans 2022 Jul 12;51(27):10501-10506. Epub 2022 Jul 12.

State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM) & Institute of Flexible Electronics (Future Technology), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, P. R. China.

Phosphorescent iridium(III) complexes have been extensively investigated as cellular imaging reagents and sensors. The intracellular localization of the complexes is known to be closely related to their formal charge, molecular size, lipophilicity, and bioactive pendants. Herein, we reported four phosphorescent iridium(III) complexes with the diimine ligands being modified with ester or amide groups as imaging reagents for living cells. The complexes have the same positive charge and very similar molecular size and weight. The lipophilicity of the complexes is similar ranging from 1.45 to 2.14. Upon internalization into living HeLa cells, while complexes 2-4, like most other iridium(III) complexes, were localized in the cytoplasm, complex 1 unexpectedly stained the whole cells including nuclei. The nuclear uptake of complex 1 was not observed when the cells were pretreated with chlorpromazine or nocodazole, suggesting that clathrin and microtubules mediated the nuclear uptake of complex 1. Additionally, the nuclear uptake efficiency is related to the cell division cycle. The complex was mainly concentrated in the nucleus when the cells were in mitosis, and distributed in whole cells when the cells were in the interphases. Furthermore, complex 1 exhibited a longer luminescence lifetime in the nucleus than in the cytoplasm as revealed by photoluminescence lifetime imaging microscopy (PLIM). Incubation of the cells in the hypoxia environment elongated the lifetime of the cytoplasmic complex, but hardly affected the luminescence properties of the intranuclear complex.
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http://dx.doi.org/10.1039/d2dt01551jDOI Listing
July 2022

Mechanism of OTUD5 in non-small cell lung cancer cell proliferation, invasion, and migration.

Bosn J Basic Med Sci 2022 Jun 27. Epub 2022 Jun 27.

Department of Oncology, Beijing Luhe Hospital Affiliated to Capital Medical University, Beijing, China.

Ovarian tumor protease deubiquitinase 5 (OTUD5) has been discussed as a regulator of cancer development. Herein, the current study set out to explore the molecular mechanism of OTUD5 in non-small cell lung cancer (NSCLC) cell proliferation, invasion, and migration. Firstly, the expression patterns of OTUD5, phosphatase and tensin homolog (PTEN), as well as microRNA (miR)-652-3p in cells were detected by qRT-PCR and Western blot. Cell viability, migration, and invasion were assessed with the help of cell-counting kit-8 and Transwell assays, in addition to the measurement of the ubiquitination and protein levels of PTEN. The binding relations between OTUD5 and PTEN, and miR-652-3p and OTUD5 were testified by co-immunoprecipitation or dual-luciferase assays. Cells were further treated with GSK2643943A (inhibitor of deubiquitinase) or miR-652-3p-inhibitor to explore the role of PTEN ubiquitination and miR-652-3p in NSCLC cells. OTUD5 and PTEN were both poorly-expressed, and miR-652-3p was highly-expressed in NSCLC cells. On the other hand, over-expression of OTUD5 suppressed NSCLC cell proliferation, invasion, and migration. OTUD5 deubiquitinated and stabilized PTEN, and miR-652-3p targeted and inhibited OTUD5 expression. Augmenting the ubiquitination levels of PTEN promoted NSCLC cell growth, whereas miR-652-3p inhibition promoted the tumor-suppressing effects of the OTUD5/ PTEN axis in NSCLC. Altogether, our findings highlighted that miR-652-3p restrained the role of OTUD5 in deubiquitinating PTEN to improve PTEN protein level, thereby promoting NSCLC cell proliferation, invasion, and migration.
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http://dx.doi.org/10.17305/bjbms.2022.7206DOI Listing
June 2022
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