Publications by authors named "Yan Deng"

533 Publications

Cooking with biomass fuels increased the risk for cognitive impairment and cognitive decline among the oldest-old Chinese adults (2011-2018): A prospective cohort study.

Environ Int 2021 May 4;155:106593. Epub 2021 May 4.

Department of Environmental Health, School of Public Health, China Medical University, Shenyang 110122, China. Electronic address:

Backgrounds: While the pernicious effects of outdoor air pollution on cognitive ability have been previously examined, evidence regarding household air pollution is scarce.

Methods: Using data from the Chinese Longitudinal Healthy Longevity Survey, we explored the relationship between cooking with biomass fuel and cognitive impairment and cognitive decline using a Cox proportional hazards model. We further assessed the correlation of biomass fuels and cognitive score using a generalized estimating equation. Cognitive ability was measured based on the Chinese version of the Mini-Mental State Examination (MMSE) and cognitive impairment was defined as MMSE < 24 points and cognitive decline was defined as a reduction of MMSE ≥ 3 points. On follow-up, we investigated the effect of switch-cooking combustibles on cognitive ability.

Results: The mean (SD) age of 4161 participants was 81.7 (10.0) years old. The reported cooking with biomass fuels was correlated with an elevated risk of cognitive impairment (hazard ratio (HR): 1.19, 95% confidence interval (CI): 1.04, 1.37) and cognitive decline (HR: 1.18, 95% CI: 1.04, 1.33). Besides, cooking with biomass fuels was related to a decrease in cognitive score (β: -0.43, 95% CI: -0.73, -0.14). In comparison to persistent biomass fuel users, participants who reported changing their primary cooking fuels from biomass to clean fuels exhibited a reduced risk of cognitive impairment (HR: 0.68, 95% CI: 0.57, 0.82) and cognitive decline (HR: 0.66, 95% CI: 0.56, 0.76) and a higher cognitive score (β: 0.72, 95% CI: 0.17, 1.26). Cooking without ventilated cookstoves was associated with a higher risk of cognitive impairment (HR: 1.31, 95% CI: 1.10, 1.58) and cognitive decline (HR: 1.18, 95% CI: 1.02, 1.38), regardless of types of cooking fuels. Interaction and stratified analyses showed relatively poor cognitive ability in participants who engaged in irregular exercise or were not living with family members.

Conclusions: Cooking with biomass fuels was correlated with a higher risk of cognitive impairment and cognitive decline. Among the oldest-old population, this risk may, however, be lower for those changing their primary cooking fuels from biomass to clean fuels.
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http://dx.doi.org/10.1016/j.envint.2021.106593DOI Listing
May 2021

Transdermal Delivery of Adipocyte Phospholipase A2 siRNA using Microneedles to Treat Thyroid Associated Ophthalmopathy-Related Proptosis.

Cell Transplant 2021 Jan-Dec;30:9636897211010633

Department of Obstetrics & Gynaecology, Faculty of Medicine, 26451The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.

Thyroid associated ophthalmopathy (TAO) is an organ-specific autoimmune disease occurring in patients with thyroid disease. Patients with TAO-related proptosis is largely due to excessive orbital adipose tissue Adipocyte phospholipase A2 (AdPLA) is one of the most important regulatory factors in adipocyte lipolysis, which may be associated with TAO-related proptosis. Thus, silencing AdPLA by RNA interference may be beneficial for the treatment of TAO. In this study, we sought to evaluate the efficiency of two types of microneedles to deliver siRNAs for silencing AdPLA. Our results showed that AdPLA mRNA was up-regulated in the orbit adipose tissues from TAO patients. Silence of AdPLA by siRNA can reduce lipid accumulation in both human and mouse adipocyte cell lines. Moreover, silence effects of silicon microneedle array patch-based and injectable microneedle device-based siRNA administration were examined at the belly site of the mice, and injectable microneedle device showed higher knockdown efficiency than silicon microneedle array patch. This study sets the stage not only for future treatment of TAO-related proptosis using AdPLA siRNA, but also provides the foundation for targeted siRNA delivery by using microneedles.
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http://dx.doi.org/10.1177/09636897211010633DOI Listing
April 2021

Fast and Accurate Control Strategy for Portable Nucleic Acid Detection (PNAD) System Based on Magnetic Nanoparticles.

J Biomed Nanotechnol 2021 Mar;17(3):407-415

Portable nucleic acid detection (PNAD) systems are performed for sample processing, amplification and detection automatically in an individual device realizing "sample in, answer out." For this goal, numerous function modules should be integrated in a diminutive device, in which temperature controller is one of the most important modules. In a nucleic acid detection process, both sample processing and polymerase chain reaction (PCR) require fast and accurate temperature control to increase concentration and purity of the extraction product and to improve amplification efficiency. In this paper, a dual-channel temperature controller for PNAD systems is developed, which contains a printed circuit board (PCB) and an integrated control program with a fast and accurate control strategy. According to the principle of nucleic acid detection based on magnetic nanoparticles, the controller can work in different modes such as high-precision heating control for nucleic acid extraction, rapid thermal cycle control for PCR, and rate adjustable constant heating/cooling control for melting curve. Evaluatively, the average heating/cooling rate of the module can exceed about 6 C/s, while the temperature fluctuation was less than ± 0.1°C, which can meet the demands of PNAD systems very well.
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http://dx.doi.org/10.1166/jbn.2021.3028DOI Listing
March 2021

Radiomics Model Based on MR Images to Discriminate Pancreatic Ductal Adenocarcinoma and Mass-Forming Chronic Pancreatitis Lesions.

Front Oncol 2021 24;11:620981. Epub 2021 Mar 24.

Sichuan Key Laboratory of Medical Imaging and Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.

Background: It is difficult to identify pancreatic ductal adenocarcinoma (PDAC) and mass-forming chronic pancreatitis (MFCP) lesions through conventional CT or MR examination. As an innovative image analysis method, radiomics may possess potential clinical value in identifying PDAC and MFCP. To develop and validate radiomics models derived from multiparametric MRI to distinguish pancreatic ductal adenocarcinoma (PDAC) and mass-forming chronic pancreatitis (MFCP) lesions.

Methods: This retrospective study included 119 patients from two independent institutions. Patients from one institution were used as the training cohort (51 patients with PDAC and 13 patients with MFCP), and patients from the other institution were used as the testing cohort (45 patients with PDAC and 10 patients with MFCP). All the patients had pathologically confirmed results, and preoperative MRI was performed. Four feature sets were extracted from T1-weighted imaging (T1WI), T2-weighted imaging (T2WI), and the artery (A) and portal (P) phases of dynamic contrast-enhanced MRI, and the corresponding radiomics models were established. Several clinical characteristics were used to discriminate PDAC and MFCP lesions, and clinical model was established. The results of radiologists' evaluation were compared with pathology and radiomics models. Univariate analysis and the least absolute shrinkage and selection operator algorithm were performed for feature selection, and a support vector machine was used for classification. The receiver operating characteristic (ROC) curve was applied to assess the model discrimination.

Results: The areas under the ROC curves (AUCs) for the T1WI, T2WI, A and, P and clinical models were 0.893, 0.911, 0.958, 0.997 and 0.516 in the primary cohort, and 0.882, 0.902, 0.920, 0.962 and 0.649 in the validation cohort, respectively. All radiomics models performed better than clinical model and radiologists' evaluation both in the training and testing cohorts by comparing the AUC of various models, all P<0.050. Good calibration was achieved.

Conclusions: The radiomics models based on multiparametric MRI have the potential ability to classify PDAC and MFCP lesions.
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http://dx.doi.org/10.3389/fonc.2021.620981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025779PMC
March 2021

COX-2 promotes the osteogenic potential of BMP9 through TGF-β1/p38 signaling in mesenchymal stem cells.

Aging (Albany NY) 2021 Apr 4;13(8):11336-11351. Epub 2021 Apr 4.

Department of Orthopedics, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.

This study investigated the effects of transforming growth factor-β1 (TGF-β1) and cyclooxygenase-2 (COX-2) on bone morphogenetic protein 9 (BMP9) in mesenchymal stem cells (MSCs). We found that BMP9 increased mRNA levels of TGF-β1 and COX-2 in C3H10T1/2 cells. BMP9-induced osteogenic markers were enhanced by TGF-β1 and reduced by TGF-βRI-specific inhibitor LY364947. BMP9 increased level of p-Smad2/3, which were either enhanced or reduced by COX-2 and its inhibitor NS398. BMP9-induced osteogenic markers were decreased by NS398 and it was partially reversed by TGF-β1. COX-2 increased BMP9-induced osteogenic marker levels, which almost abolished by LY364947. BMP9-induced bone formation was enhanced by TGF-β1 but reduced by silencing TGF-β1 or COX-2. BMP9's osteogenic ability was inhibited by silencing COX-2 but partially reversed by TGF-β1. TGF-β1 and COX-2 enhanced activation of p38 signaling, which was induced by BMP9 and reduced by LY364947. The ability of TGF-β1 to increase the BMP9-induced osteogenic markers was reduced by p38-specific inhibitor, while BMP9-induced TGF-β1 expression was reduced by NS398, but enhanced by COX-2. Furthermore, CREB interacted with Smad1/5/8 to regulate TGF-β1 expression in MSCs. These findings suggest that COX-2 overexpression leads to increase BMP9's osteogenic ability, resulting from TGF-β1 upregulation which then activates p38 signaling in MSCs.
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http://dx.doi.org/10.18632/aging.202825DOI Listing
April 2021

Manipulating mode degeneracy for tunable spectral characteristics in multi-microcavity photonic molecules.

Opt Express 2021 Mar;29(7):11181-11193

Optical microcavities are capable of confining light to a small volume, which could dramatically enhance the light-matter interactions and hence improve the performances of photonic devices. However, in the previous works on the emergent properties with photonic molecules composed of multiple plasmonic microcavities, the underlying physical mechanism is unresolved, thereby imposing an inevitable restriction on manipulating degenerate modes in microcavity with outstanding performance. Here, we demonstrate the mode-mode interaction mechanism in photonic molecules composed of degenerate-mode cavity and single-mode cavity through utilizing the coupled mode theory. Numerical and analytical results further elucidate that the introduction of direct coupling between the degenerate-mode cavity and single-mode cavity can lift the mode degeneracy and give rise to the mode splitting, which contributes to single Fano resonance and dual EIT-like effects in the double-cavity photonic molecule structure. Four times the optical delay time compared to typical double-cavity photonic molecule are achieved after removing the mode degeneracy. Besides, with the preserved mode degeneracy, ultra-wide filtering bandwidth and high peak transmission is obtained in multiple-cavity photonic molecules. Our results provide a broad range of applications for ultra-compact and multifunction photonic devices in highly integrated optical circuits.
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http://dx.doi.org/10.1364/OE.420462DOI Listing
March 2021

Effects of meteorological factors and groundwater depths on plant sap flow velocities in karst critical zone.

Sci Total Environ 2021 Mar 27;781:146764. Epub 2021 Mar 27.

School of Electronic Engineering and Automation, Guilin University of Electronic Technology, Guilin 541004, China.

Determining water supply intensity of fracture/conduits is one of the difficulties involved in the research of plant transpiration water consumption in the Karst Critical Zone (KCZ). Our aims were to evaluate the effect of groundwater depth on plant sap flow velocities in KCZ. Thus, four sampled plots with different groundwater depth (GD) in boreholes KCZ7 (4 to 10 m GD), KCZ5 (2 to 9 m GD), KCZ1 (0 to 8 m GD) and KCZ3 (2 to 5 m GD), were selected, and the plant stem sap flow velocity in each plot were also monitored continuously and automatically using heat ratio techniques. The daily sap flow flux of Toona sinensis varied between 0.35 kg d in KCZ3 and 1.50 kg d in KCZ1. Photosynthetically active radiation (PAR), vapor pressure deficit (VPD), and gust velocity (ZWS) were the primary meteorological factors that determined the sap flow velocity of T. sinensis, which contributed to a regression equation, while the influence of GD on sap flow was complex. Most of the sap flow velocity had no obvious significant correlation with the GD; however, the sap flow velocity in four different GD showed significant differences (P < 0.05). Unit sap flow velocity changes induced by unit GD changes (K) in KCZ7 and KCZ1 samples was faster than that of other samples. In brief, the sap flow velocity was mainly affected by the PAR and VPD in KCZ7, KCZ5 and KCZ1 because of the sufficient epikarst water, while the sap flow velocity in KCZ3 was mainly affected by the rock water content. The karst aquifer medium and GD was the main factors causing the difference sap flow velocity in the four sample plots. This finding indicated that KCZ aquifer medium structure may have an important influence on plant water utilization.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146764DOI Listing
March 2021

Effects of Water and Energy on Plant Diversity along the Aridity Gradient across Dryland in China.

Plants (Basel) 2021 Mar 27;10(4). Epub 2021 Mar 27.

State Key Laboratory of Grassland Agro-Ecosystem, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.

Plants need water and energy for their growth and reproduction. However, how water and energy availability influence dryland plant diversity along the aridity gradient in water-limited regions is still lacking. Hence, quantitative analyses were conducted to evaluate the relative importance of water and energy to dryland plant diversity based on 1039 quadrats across 184 sites in China's dryland. The results indicated that water availability and the water-energy interaction were pivotal to plant diversity in the entire dryland and consistent with the predictions of the water-energy dynamic hypothesis. The predominance of water limitation on dryland plant diversity showed a weak trend with decreasing aridity, while the effects of energy on plants were found to be significant in mesic regions. Moreover, the responses of different plant lifeforms to water and energy were found to vary along the aridity gradient. In conclusion, the study will enrich the limited knowledge about the effects of water and energy on plant diversity (overall plants and different lifeforms) in the dryland of China along the aridity gradient.
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http://dx.doi.org/10.3390/plants10040636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067162PMC
March 2021

The Prevalence of Mild Cognitive Impairment among Chinese People: A Meta-Analysis.

Neuroepidemiology 2021 23;55(2):79-91. Epub 2021 Mar 23.

Department of Nutrition Hygiene and Toxicology, School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan, China,

Background: Mild cognitive impairment (MCI) induced the majority number of dementia patients. The prevalence of MCI in China varied across studies with different screening tools and diagnostic criteria.

Objective: A systematic review and meta-analysis was conducted to estimate the pooled MCI prevalence among the population aged 55 years and older in China.

Methods: PubMed, EMBASE, CNKI, Wanfang, CQVIP, and CBMdisc were searched for studies on prevalence of MCI among Chinese elderly between January 1, 1980, and February 10, 2020. The quality assessment was conducted via external validity, internal validity, and informativity, the pooled prevalence was calculated through the random-effect model, and the homogeneity was evaluated by Cochran's Q test and I2.

Results: Fifty-three studies with 123,766 subjects were included. The pooled prevalence of MCI among Chinese elderly was 15.4% (95% CI: 13.5-17.4%). Subgroup analyses indicated that the prevalence calculated with different screening tools was 20.2% (95% CI: 15.1-25.9%) for Montreal Cognitive Assessment (MoCA) and 13.0% (95% CI: 10.7-15.5%) for Mini-Mental State Examination (MMSE). According to different diagnostic criteria, the prevalence was 14.8% (95% CI: 12.2-17.6%) for Petersen criteria, 15.0% (95% CI: 12.7-17.5%) for DSM-IV, and 21.2% (95% CI: 17.5-25.2%) for Chinese Expert Consensus on Cognitive Impairment (CECCI). Besides, women, older adults, illiterate people, rural residents, and those who lived with unhealthy lifestyles and morbidity showed higher prevalence.

Conclusions: The prevalence of MCI in China was 15.4%, which varied by demographics, lifestyles, morbidity, screening tools, and diagnostic criteria. In further studies, screening tools and diagnosis criteria should be considered when estimating MCI prevalence.
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http://dx.doi.org/10.1159/000512597DOI Listing
March 2021

The association of CYP2D6 gene polymorphisms in the full-length coding region with higher recurrence rate of vivax malaria in Yunnan Province, China.

Malar J 2021 Mar 20;20(1):160. Epub 2021 Mar 20.

Yunnan Institute of Parasitic Diseases Control, Yunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Yunnan Centre of Malaria Research, Pu'er, 665000, China.

Background: Accumulating evidence suggest that compromised CYP2D6 enzyme activity caused by gene mutation could contribute to primaquine failure for the radical cure of vivax malaria. The current study aims to preliminarily reveal the association between the recurrence of vivax malaria in Yunnan Province and CYP2D6 gene mutation by analysing polymorphisms in the entire coding region of human CYP2D6 gene.

Methods: Blood samples were collected from patients with vivax malaria, who received "chloroquine and 8-day course of primaquine therapy" in Yunnan Province. The suspected relapsed cases were determined by epidemiological approaches and gene sequence alignment. PCR was conducted to amplify the CYP2D6 gene in the human genome, and the amplified products were then sequenced to compare with the non-mutation "reference" sequence, so as to ensure correct sequencing results and to determine 9 exon regions. Subsequently, the DNA sequences of 9 exons were spliced into the coding DNA sequence (CDS), which, by default, is known as maternal CDS. The paternal CDS was obtained by adjusting the bases according to the sequencing peaks. The mutation loci, haplotypes (star alleles), genotypes and odds ratios (OR) of all the CDSs were analysed.

Results: Of the119 maternal CDS chains in total with 1491 bp in length, 12 mutation sites in the 238 maternal and paternal CDS chains were detected. The c.408G > C mutation was most frequently detected in both suspected relapsed group (SR) and non-relapsed group (NR), reaching 85.2% (75/88) and 76.0% (114/150), respectively. The c.886C > T mutation was most closely related to the recurrence of vivax malaria (OR = 2.167, 95% CI 1.104-4.252, P < 0.05). Among the 23 haplotypes (Hap_1 ~ Hap_23), Hap_3 was non-mutant, and the sequence structure of Hap_9 was the most complicated one. Five star alleles, including *1, *2, *4, *10 and *39, were confirmed by comparison, and CYP2D6*10 allele accounted for the largest percentage (45.4%, 108/238). The frequency of CYP2D6*2 allele in the SR group was significantly higher than that in the NR group (Χ = 16.177, P < 0.05). Of the defined 24 genotypes, 8 genotypes, including *4/*4, *4/*o, *2/*39, *39/*m, *39/*x, *1/*r, *1/*n, and *v/*10, were detected only in the SR group.

Conclusion: Mutation of CYP2D6*10 allele accounts for the highest proportion of vivax malaria cases in Yunnan Province. The mutations of c. 886C > T and CYP2D6*2 allele, which correspond to impaired PQ metabolizer phenotype, are most closely related to the relapse of vivax malaria. In addition, the genotype *4/*4 with null CYP2D6 enzyme function was only detected in the SR group. These results reveal the risk of defected CYP2D6 enzyme activity that diminishes the therapeutic effect of primaquine on vivax malaria.
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http://dx.doi.org/10.1186/s12936-021-03685-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981985PMC
March 2021

The Association of Socioeconomic Status with the Burden of Cataract-related Blindness and the Effect of Ultraviolet Radiation Exposure: An Ecological Study.

Biomed Environ Sci 2021 Feb;34(2):101-109

Department of Environmental Health, School of Public Health, China Medical University, Shenyang 110122, Liaoning, China.

Objective: To assess the association of socioeconomic status with the burden of cataract blindness in terms of year lived with disability (YLD) rates and to determine whether ultraviolet radiation (UVR) levels modify the effect of socioeconomic status on this health burden.

Methods: National and subnational age-standardized YLD rates associated with cataract-related blindness were derived from the Global Burden of Disease (GBD) study 2017. The human development index (HDI) from the Human Development Report was used as a measure of socioeconomic status. Estimated ground-level UVR exposure was obtained from the Ozone Monitoring Instrument (OMI) dataset of the National Aeronautics and Space Administration (NASA).

Results: Across 185 countries, socioeconomic status was inversely associated with the burden of cataract blindness. Countries with a very high HDI had an 84% lower age-standardized YLD rate [95% confidence interval ( ): 60%-93%, < 0.001] than countries with a low HDI; for high-HDI countries, the proportion was 76% (95% : 53%-88%, < 0.001), and for medium-HDI countries, the proportion was 48% (95% : 15%-68%, = 0.010; for trend < 0.001). The interaction analysis showed that UVR exposure played an interactive role in the association between socioeconomic status and cataract blindness burden ( value for interaction = 0.047).

Conclusion: Long-term high-UVR exposure amplifies the association of poor socioeconomic status with the burden of cataract-related blindness. The findings emphasize the need for strengthening UVR exposure protection interventions in developing countries with high-UVR exposure.
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http://dx.doi.org/10.3967/bes2021.015DOI Listing
February 2021

An autophagy-related prognostic signature associated with immune microenvironment features of uveal melanoma.

Biosci Rep 2021 Mar;41(3)

Department of Pediatric Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.

Autophagy is involved in cancer initiation and progression but its role in uveal melanoma (UM) was rarely investigated. Herein, we built an autophagy-related gene (ARG) risk model of UM patients by univariate Cox regression and least absolute shrinkage and selection operator (Lasso) regression model and filtrated out nine prognostic ARGs in The Cancer Genome Atlas (TCGA) cohort. Survival and Receiver Operating Characteristic (ROC) Curve analysis in the TCGA and other four independent UM cohorts (GSE22138, GSE27831, GSE44295 and GSE84976) proved that the ARG-signature possessed robust and steady prognosis predictive ability. We calculated risk scores for patients included in our study and patients with higher risk scores showed worse clinical outcomes. We found the expressions of the nine ARGs were significantly associated with clinical and molecular features (including risk score) and overall survival (OS) of UM patients. Furthermore, we utilized univariate and multivariate Cox regression analyses to determine the independent prognostic ability of the ARG-signature. Functional enrichment analysis showed the ARG-signature was correlated with several immune-related processes and pathways like T-cell activation and T-cell receptor signaling pathway. Gene set enrichment analysis (GSEA) found tumor hallmarks including angiogenesis, IL6-JAK-STAT3-signaling, reactive oxygen species pathway and oxidative phosphorylation were enriched in high-risk UM patients. Finally, infiltrations of several immune cells and immune-related scores were found significantly associated with the ARG-signature. In conclusion, the ARG-signature might be a strong predictor for evaluating the prognosis and immune infiltration of UM patients.
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http://dx.doi.org/10.1042/BSR20203812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982771PMC
March 2021

Unsuspected Bladder Diverticulum Resulted in False-Positive 68Ga-DOTATATE Uptake.

Clin Nucl Med 2021 06;46(6):477-479

Department of Pediatrics, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.

Abstract: A 67-year-old woman with medullary thyroid cancer was referred for a 68Ga-DOTATATE PET/CT scan for staging. The images showed abnormal activity in the left thyroid gland and the left cervical lymph nodes. Surprisingly, increased activity was also noted in the posterolateral aspect of the urinary bladder without corresponding anatomical abnormality. This activity disappeared after urination, suggesting false-positive uptake due to bladder diverticulum.
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http://dx.doi.org/10.1097/RLU.0000000000003571DOI Listing
June 2021

PTEN Reduces BMP9-Induced Osteogenic Differentiation Through Inhibiting Wnt10b in Mesenchymal Stem Cells.

Front Cell Dev Biol 2020 4;8:608544. Epub 2021 Feb 4.

Department of Pharmacology, School of Pharmacy, Chongqing Medical University, Chongqing, China.

Bone morphogenetic protein 9 (BMP9) is one of the most efficacious osteogenic cytokines. PTEN and Wnt10b are both implicated in regulating the osteogenic potential of BMP9, but the potential relationship between them is unknown. In this study, we determined whether PTEN could reduce the expression of Wnt10b during the osteogenic process initialized by BMP9 in mesenchymal stem cells (MSCs) and the possible molecular mechanism. We find that PTEN is inhibited by BMP9 in MSCs, but Wnt10b is increased simultaneously. The BMP9-induced osteogenic markers are reduced by PTEN but increased by silencing PTEN. The effects of knockdown PTEN on elevating BMP9-induced osteogenic markers are almost abolished by knockdown of Wnt10b. On the contrary, the BMP9-increased ALP activities and mineralization are both inhibited by PTEN but almost reversed by the combination of Wnt10b. Bone masses induced by BMP9 are enhanced by knockdown of PTEN, which is reduced by knockdown of Wnt10b. The BMP9-increased Wnt10b is decreased by PTEN but enhanced by knockdown of PTEN. Meanwhile, the BMP9-induced Wnt10b is also reduced by a PI3K-specific inhibitor (Ly294002) or rapamycin, respectively. The BMP9-induced phosphorylation of CREB or Smad1/5/9 is also reduced by PTEN, but enhanced by PTEN knockdown. In addition, p-CREB interacts with p-Smad1/5/9 in MSCs, and p-CREB or p-Smad1/5/9 are both enriched at the promoter region of Wnt10b. Our findings indicate that inhibitory effects of PTEN on BMP9's osteogenic potential may be partially mediated through decreasing the expression of Wnt10b via the disturbance of interaction between CREB and BMP/Smad signaling.
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http://dx.doi.org/10.3389/fcell.2020.608544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889951PMC
February 2021

Point-of-care diagnostics for infectious diseases: From methods to devices.

Nano Today 2021 Apr 6;37:101092. Epub 2021 Feb 6.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.

The current widespread of COVID-19 all over the world, which is caused by SARS-CoV-2 virus, has again emphasized the importance of development of point-of-care (POC) diagnostics for timely prevention and control of the pandemic. Compared with labor- and time-consuming traditional diagnostic methods, POC diagnostics exhibit several advantages such as faster diagnostic speed, better sensitivity and specificity, lower cost, higher efficiency and ability of on-site detection. To achieve POC diagnostics, developing POC detection methods and correlated POC devices is the key and should be given top priority. The fast development of microfluidics, micro electro-mechanical systems (MEMS) technology, nanotechnology and materials science, have benefited the production of a series of portable, miniaturized, low cost and highly integrated POC devices for POC diagnostics of various infectious diseases. In this review, various POC detection methods for the diagnosis of infectious diseases, including electrochemical biosensors, fluorescence biosensors, surface-enhanced Raman scattering (SERS)-based biosensors, colorimetric biosensors, chemiluminiscence biosensors, surface plasmon resonance (SPR)-based biosensors, and magnetic biosensors, were first summarized. Then, recent progresses in the development of POC devices including lab-on-a-chip (LOC) devices, lab-on-a-disc (LOAD) devices, microfluidic paper-based analytical devices (PADs), lateral flow devices, miniaturized PCR devices, and isothermal nucleic acid amplification (INAA) devices, were systematically discussed. Finally, the challenges and future perspectives for the design and development of POC detection methods and correlated devices were presented. The ultimate goal of this review is to provide new insights and directions for the future development of POC diagnostics for the management of infectious diseases and contribute to the prevention and control of infectious pandemics like COVID-19.
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http://dx.doi.org/10.1016/j.nantod.2021.101092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864790PMC
April 2021

Soil Microbial Composition and Gene Abundance Are Sensitive to Phosphorus Level in a Long-Term Wheat-Maize Crop System.

Front Microbiol 2020 14;11:605955. Epub 2021 Jan 14.

College of Resources and Environment, Academy of Agricultural Sciences, Southwest University, Chongqing, China.

Microbes associated with phosphorus (P) cycling are intrinsic to soil P transformation and availability for plant use but are also influenced by the application of P fertilizer. Nevertheless, the variability in soil P in the field means that integrative analyses of soil P cycling, microbial composition, and microbial functional genes related to P cycling remain very challenging. In the present study in the North China Plain, we subjected the bacterial and fungal communities to amplicon sequencing analysis and characterized the alkaline phosphatase ( encoding bacterial alkaline phosphatase in a long-term field experiment (10 years) with six mineral P fertilization rates up to 200 kg P ha. Long-term P fertilization increased soil available P, inorganic P, and total P, while soil organic P increased until the applied P rate reached 25 kg ha and then decreased. The fungal alpha-diversity decreased as P rate increased, while there were no significant effects on bacterial alpha-diversity. Community compositions of bacteria and fungi were significantly affected by P rates at order and family levels. The number of keystone taxa decreased from 10 to 3 OTUs under increasing P rates from 0 to 200 kg ha. The gene copy numbers of the biomarker of the alkaline phosphatase was higher at moderate P rates (25 and 50 kg ha) than at low (0 and 12.5 kg ha) and high (100 and 200 kg ha) rates of P fertilization, and was positively correlated with soil organic P concentration. One of the keystone taxa named BacOTU3771 belonging to Xanthomonadales was positively correlated with potential functional genes encoding enzymes such as glycerophosphoryl diester phosphodiesterase, acid phosphatase and negatively correlated with guinoprotein glucose dehydrogenase. Altogether, the results show the systematic effect of P gradient fertilization on P forms, the microbial community structure, keystone taxa, and functional genes associated with P cycling and highlight the potential of moderate rates of P fertilization to maintain microbial community composition, specific taxa, and levels of functional genes to achieve and sustain soil health.
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http://dx.doi.org/10.3389/fmicb.2020.605955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873961PMC
January 2021

Indoor solid fuel use and incident arthritis among middle-aged and older adults in rural China: A nationwide population-based cohort study.

Sci Total Environ 2021 Jun 27;772:145395. Epub 2021 Jan 27.

Department of Immunology and Rheumatology, First Hospital, China Medical University, Shenyang 110001, China. Electronic address:

Background: Many households in developing countries, including China, rely on the traditional use of solid fuels for cooking and heating. Arthritis is highly prevalent in middle-aged and older adults and is a major cause of disability. However, evidence linking indoor solid fuel use with arthritis is scarce in this age group (≥45 years) in developing countries.

Objectives: To investigate whether exposure to indoor solid fuel for cooking and heating is associated with arthritis in middle-aged and older adults in rural China.

Methods: Data for the present study were extracted from the China Health and Retirement Longitudinal Study (CHARLS), a longitudinal national prospective study of adults aged 45 years and older enrolled in 2010 and followed up through 2015. We included 7807 rural participants without arthritis at baseline, of whom 1548 living in a central heating area in winter were included in the heating analysis (taking the Qinling-Huaihe line as the heating boundary). Cox proportional hazards models were used to examine the association between indoor solid fuel use and arthritis, controlling for age, sex, education, marital status, smoking status, drinking status, self-reported socioeconomic status, BMI, sleep time, napping time, independent cooking, hypertension, diabetes, dyslipidemia, heart problems and stroke. We also investigated the effect of switching primary fuels and using solid fuels for both cooking and heating on arthritis risk.

Results: The mean (SD) age of the study participants was 59.2 (10.0) years old, and 48.0% of participants were women. A total of 64.8% and 63.0% of the participants reported primarily using solid fuel for cooking and heating, respectively. Arthritis incidence rates were lower among clean fuel users than solid fuel users. Compared to those using clean fuels, cooking and heating solid fuel users had a higher risk of arthritis, with hazard ratios (HRs) of 1.22 (95% confidence interval (CI): 1.01, 1.49) and 1.76 (95% CI: 1.07, 2.89), respectively. Switching from clean fuels to solid fuels for heating (HR: 3.28, 95% CI: 1.21, 7.91) and using solid fuels for both cooking and heating (HR, 1.71, 95% CI, 1.01-2.79) increased the risk of arthritis.

Conclusions: Long-term solid fuel use for indoor cooking and heating is associated with an increased risk of arthritis events among adults aged 45 years and older in rural China. The potential benefits of reducing indoor solid fuel use in groups at high risk for arthritis merit further exploration.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145395DOI Listing
June 2021

Novel Genetic Variants of Promoter in Gestational Diabetes Mellitus and its Molecular Regulation in Adipogenesis.

Front Endocrinol (Lausanne) 2020 22;11:499788. Epub 2021 Jan 22.

Department of Obstetrics and Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong.

Peroxisome proliferator-activated receptor γ2 () is a nuclear hormone receptor of ligand-dependent transcription factor with a key role in adipogenesis and insulin sensitization in diabetes mellitus. In this study, we investigated genetic variants in promoter, its association with gestational diabetes mellitus (GDM), and its molecular regulation. promoter and start codon (-2,091 to +82 bp) from 400 pregnancies with GDM and 400 gestational-age matched control pregnancies were sequenced. Association and linkage disequilibrium of the identified polymorphisms with GDM was determined. ChIP-seq, gene silencing, and dual-luciferase reporter assays were performed to confirm transcription factor binding sites and promoter activity of the variants. Transfection experiments were carried out to determine the effects of variants on gene expression and adipogenesis. Among 15 variants identified, 7 known variants were not significantly associated with the risk of GDM (odds ratio: 0.710-1.208, 95% confidence interval: 0.445-0.877 to 1.132-1.664, > 0.05) while linkage disequilibrium was significant (D' > 0.7, R > 0.9). However, T-A-A-T-G haplotype was not significantly associated with GDM (χ = 2.461, = 0.117). Five rare variants and 3 novel variants (rs948820149, rs1553638909, and rs1553638903) were only found in GDM. Transcription factor glucocorticoid receptor β (GRβ) bound to -807A/C (rs948820149) and knockdown of GRβ suppressed promoter activity. This mutation significantly down-regulated expression and alleviated adipogenesis. In conclusion, a novel -807A/C in promoter was identified in Chinese women with GDM and the mutation affected GRβ binding and transcription of for adipogenesis.
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http://dx.doi.org/10.3389/fendo.2020.499788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862745PMC
January 2021

Is polycystic ovary syndrome appropriately diagnosed by obstetricians and gynaecologists across China: a nationwide survey.

J Ovarian Res 2021 Feb 3;14(1):25. Epub 2021 Feb 3.

Department of Obstetrics and Gynecology, Peking Union Medical College, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No.1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China.

Background: To describe the diagnostic criteria used and their application accuracy in the practice of polycystic ovary syndrome (PCOS) caring among obstetricians and gynaecologists across China.

Methods: This was an Online cross-sectional survey of Obstetricians and gynecologists involved in PCOS caring conducted via the largest continuing education platform of obstetrics and gynecology across China from September 2019 to November 2019.

Results: A total of 2,328 respondents were eligible for the final analysis. Of these, 94.5 % were general obstetricians and gynaecologists (Ge-ObGyn), and 5.5 % were reproductive endocrinologists (Re-ObGyn). Overall, the most frequently used criteria were the Androgen Excess and Polycystic Ovary Syndrome Society (AE-PCOS) criteria (48.2 %), followed by the Rotterdam criteria (35.7 %) and NIH criteria (12.1 %). Of the respondents, 31.3 % used their diagnostic criteria in their clinical practice. More respondents who chose the Rotterdam criteria could accurately apply the diagnostic criteria than those who chose the AE-PCOS criteria (41.2 % vs. 32.1 %, P < 0.001). Compared with Ge-ObGyn, Re-ObGyn were less likely to use the AE-PCOS criteria (adjusted odds ratio, 0.513; 95 % CI, 0.328-0.802; P < 0.05) and 1.492 times more likely to accurately use their criteria (95 % CI, 1.014-2.196; P < 0.05).

Conclusions: Less than one-third of obstetricians and gynaecologists across China could accurately use the diagnostic criteria they choose to diagnose PCOS. There is an urgent need to train obstetricians and gynaecologists on PCOS diagnosis in an effort to improve the medical care quality of patients with PCOS.
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http://dx.doi.org/10.1186/s13048-021-00780-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7860004PMC
February 2021

Ultrasensitive chemiluminescence immunoassay with enhanced precision for the detection of cTnI amplified by acridinium ester-loaded microspheres and internally calibrated by magnetic fluorescent nanoparticles.

Nanoscale 2021 Feb 3;13(5):3275-3284. Epub 2021 Feb 3.

State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

A novel enhanced chemiluminescent immunoassay (CLIA) for ultrasensitive and excellent precisive determination of cardiac troponin I (cTnI) was reported. The method made full use of poly[(N-isopropyl acrylamide)-co-(methacrylic acid)] (P(NIPAM-co-MAA)) microspheres as new potential signal enhancers and magnetic fluorescent nanoparticles as internal standards for better precision. This protocol involved a sandwich format, in which the antigen in the sample was captured by the immobilized antibodies on the surface of magnetic fluorescent beads and recognized by the other antibodies labeled with acridinium ester (AE)-loaded P(NIPAM-co-MAA) microspheres. The combination of the remarkable sensitivity of the enhanced CLIA method and the use of P(NIPAM-co-MAA) microspheres as anti-cTnI carriers for acridinium ester signal amplification provided an extremely sensitive limit of blank (LoB) at 0.097 pg mL, a limit of detection (LoD) at 0.116 pg mL, and a limit of quantitation (LoQ) at 0.606 pg mL, much greater than those achieved by the classical chemiluminescence immunoassay (CLIA, Getein). Moreover, the intra-day variable coefficient can be improved to 1.21-2.12%, and inter-day variability was 2.01-3.49% under the application of magnetic fluorescent beads as an internal standard. The sensitivity and precision have reached a high level, comparable with the current commercial detection kits. The results showed a good correlation with a commercial chemiluminescence assay (CLIA, Abbott), with a correlation coefficient of 0.9883. This proposed method has been successfully applied to the clinical determination of cTnI in the human serum.
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http://dx.doi.org/10.1039/d0nr08008jDOI Listing
February 2021

Pulmonary mucosa-associated lymphoid tissue lymphoma: CT findings and pathological basis.

J Surg Oncol 2021 Apr 1;123(5):1336-1344. Epub 2021 Feb 1.

Department of Respiratory and Critical Care, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Background: Pulmonary mucosa-associated lymphoid tissue lymphoma (MALToma) is the most frequent subset of primary pulmonary lymphoma. This study aimed to identify radiologic characteristics of pulmonary MALToma based on computed tomography (CT) observations and pathologic features, and further investigate its prognosis.

Methods: Sixty-six patients (55.4 ± 10.9 years; 51.5% male) diagnosed as pulmonary MALToma by pathology were retrospectively enrolled. According to distributions and features of lesions shown on CT, patients were divided into three patterns, including single nodular/mass, multiple nodular/mass, and pneumonia-like consolidative.

Results: Variety of the location and extent of the lymphomatous infiltration accounted for different characteristics demonstrated at CT. The pneumonia-like consolidative pattern was the most frequent pattern observed in 42 patients (63.6%), followed by single nodular/mass (21.2%) and multiple nodular/mass (15.2%). CT features included air bronchogram (72.7%), well-marginated halo sign (53.0%), coarse spiculate with different lengths (72.7%), angiogram sign (77.1% of 35 patients), peribronchovascular thickening (48.5%), irregular cavitation (16.7%) and pulmonary cyst (7.6%). The estimated 5-year cumulative overall survival rate of pulmonary MALToma was 100.0%.

Conclusions: Pulmonary MALToma demonstrates several characteristics at CT. Identification of the significant pulmonary abnormalities of this indolent disease entity might be helpful for early diagnosis and optimal treatment.
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http://dx.doi.org/10.1002/jso.26403DOI Listing
April 2021

Clinical-Grade Human Embryonic Stem Cell-Derived Mesenchymal Stromal Cells Ameliorate the Progression of Osteoarthritis in a Rat Model.

Molecules 2021 Jan 24;26(3). Epub 2021 Jan 24.

Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing 100044, China.

Mesenchymalstem cell (MSC)-based therapy is being increasingly explored in preclinical and clinical studies as a regenerative method for treating osteoarthritis (OA). However, the use of primary MSCs is hampered by a number of limitations, including donor heterogeneity and inconsistent cell quality. Here, we tested the therapeutic potential of embryonic stem cell-derived MSCs (ES-MSCs) in anOA rat model. ES-MSCs were generated and identified by morphology, trilineage differentiation and flow cytometry. Sprague Dawley rats were treated with either a single dose (10 cells/rat) of ES-MSCs or with three doses spaced one week apart for each dose, starting at four weeks after anterior cruciate ligament transectionto induce OA. Cartilage quality was evaluated at 6 and 10 weeks after treatment with behavioral analysis, macroscopic examination, and histology. At sixweeks after treatment, the groups treated with both single and repeated doses of ES-MSCs had significantly better modified Mankin scores and International Cartilage Repair Society (ICRS) macroscopic scores in the femoral condyle compared to the control group. At 10 weeks after treatment, the repeated doses group had a significantly better ICRS macroscopic scores in the femoral condyle compared to the single dose and control groups. Histological analysis also showed more proteoglycan and less cartilage loss, along with lower Mankin scores in the repeated doses group. In conclusion, treatment with multiple injections of ES-MSCs can ameliorate OA in a rat model. TheES-MSCs have potential to be considered as a regenerative therapy for OA, and can provide an infinite cellular source.
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http://dx.doi.org/10.3390/molecules26030604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865331PMC
January 2021

Immunogenicity and safety of the inactivated poliomyelitis vaccine made from Sabin strains in a phase IV clinical trial for the vaccination of a large population.

Vaccine 2021 Mar 22;39(9):1463-1471. Epub 2021 Jan 22.

Institute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Kunming, Yunnan, China; Yunnan Key Laboratory of Vaccine Research and Development on Severe Infections Diseases, Kunming, Yunnan, China. Electronic address:

As a recently launched novel vaccine used as one of the vaccines for the final eradication of polios worldwide, complete data on the consistency and immunogenicity characteristics of the inactivated poliomyelitis vaccine made from the Sabin strain (sIPV) and its safety in large-scale populations are required to support the future use of this vaccine worldwide. A phase IV clinical trial was conducted to perform an immunogenicity evaluation of lot-to-lot consistency of three commercial batches of sIPV in 1200 infants and to investigate the vaccine's safety on a large-scale in 20,019 infants for active monitoring and 29,683 infants for passive monitoring through the Adverse Event Following Immunization (AEFI) reporting system in China. In the immunogenicity evaluation, the average seroconversion rates for type I, type II and type III of the three groups were 99.83%, 98.93% and 99.44%, respectively. No differences in the seroconversion rate and the GMT ratios were noted in the pair-to-pair comparisons. In the large-scale safety evaluation, most adverse reactions occurred 0-30 days after the first doses, and the common local and systemic reactions were similar to those in the phase III clinical trial, with low incidence in both activated and passive monitoring. In conclusion, sIPV exhibits good lot-to-lot consistency and safety in large-scale populations; thus, it is qualified to serve as one of the vaccines for use in eradicating all wild and vaccine-derived polioviruses worldwide in the near future. Clinic Trial Registration. NCT04224519 and NCT04220515.
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http://dx.doi.org/10.1016/j.vaccine.2021.01.027DOI Listing
March 2021

Icarisid II promotes proliferation and neuronal differentiation of neural stem cells via activating Wnt/β-catenin signaling pathway.

Phytother Res 2021 Jan 17. Epub 2021 Jan 17.

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.

Adult neurogenesis plays a vital role in maintaining cognitive functions in mammals and human beings. Mobilization of hippocampal neurogenesis has been regarded as a promising therapeutic approach to restore injured neurons in neurodegenerative diseases including Alzheimer's disease (AD). Icarisid II (ICS II), an active ingredient derived from Epimedii Folium, has been reported to exhibit multiple neuroprotective effects. In the present study, we investigated the effects of ICS II on the proliferation and differentiation of neural stem cells (NSCs) and amyloid precusor protein (APP)-overexpressing NSCs (APP-NSCs) in vitro. Our results demonstrated that ICS II dose-dependently suppressed apoptosis and elevated viability of APP-NSCs. ICS II (1 μM) potently promoted proliferation and neuronal differentiation of NSCs and APP-NSCs. ICS II (1 μM) significantly upregulated Wnt-3a expression, increased the phosphorylation of glycogen synthase kinase-3β and enhanced the nuclear transfer of β-catenin. Moreover, ICS II also promoted astrocytes to secrete Wnt-3a, which positively modulates Wnt/β-catenin signaling pathway. These findings demonstrate that ICS II promotes NSCs proliferation and neuronal differentiation partly by activating the Wnt/β-catenin signaling pathway.
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http://dx.doi.org/10.1002/ptr.7022DOI Listing
January 2021

Pre-emptive remifentanil alleviates pain associated with tracheal suctioning in patients under mechanical ventilation and goal-directed sedation: A randomized controlled feasibility trial.

Int J Nurs Pract 2021 Jan 5:e12915. Epub 2021 Jan 5.

Department of Emergency Medicine, Laboratory of Emergency Medicine, West China Hospital, and Disaster Medical Center, Sichuan University, Chengdu, China.

Aims: To investigate the efficacy of pre-emptive remifentanil in alleviating pain during tracheal suctioning in patients under mechanical ventilation.

Background: Goal-directed sedation is recommended for patients under mechanical ventilation by the current guidelines. Whether goal-directed sedation can prevent pain during tracheal suctioning in these patients is unknown.

Design: This was a two-centre, randomized, crossover, single-blind trial conducted between August and October 2019.

Methods: Patients under mechanical ventilation received low-dose remifentanil, high-dose remifentanil or placebo prior to each tracheal suctioning in a random order. The primary outcomes were evaluated using the critical-care pain observation tool and Richmond agitation-sedation scale after tracheal suctioning. Adverse events were also documented.

Results: A total of 39 patients who underwent 117 tracheal suctions were enrolled. After the tracheal suction, changes in the critical-care pain observation tool and Richmond agitation-sedation scale scores were significantly lower in the low-dose and high-dose groups than in the placebo group (P < 0.001). A non-significant increase in the absence of spontaneous breathing was observed in the high-dose group compared to that in the placebo group.

Conclusion: A pre-emptive remifentanil bolus of 0.5 μg/kg can mitigate the pain associated with tracheal suctioning.
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http://dx.doi.org/10.1111/ijn.12915DOI Listing
January 2021

Dynamics of Internalization and Intracellular Interaction of Tau Antibodies and Human Pathological Tau Protein in a Human Neuron-Like Model.

Front Neurol 2020 26;11:602292. Epub 2020 Nov 26.

Department of Neuroscience and Physiology, Neuroscience Institute, New York University Grossman School of Medicine, New York, NY, United States.

We and others have shown in various and cell culture models that several tau antibodies interact with pathological tau within neurons. To further clarify this interaction in a dynamic human model, we differentiated SH-SY5Y cells with retinoic acid and BDNF to create a neuron-like model. Therein, tau antibodies were primarily taken up by receptor-mediated endocytosis, and prevented toxicity of human brain-derived paired helical filament-enriched tau (PHF). Subsequently, we monitored in real-time the interaction of antibodies and PHF within endocytic cellular compartments. Cells were pre-treated with fluorescently-tagged PHF and then incubated with tau antibodies, 4E6, 6B2, or non-specific isotype control IgG1 labeled with a pH sensitive dye. The uptake and binding of the efficacious antibody, 4E6, to PHF occurred mainly within the soma, whereas the ineffective antibody, 6B2, and ineffective control IgG1, were visualized via the processes and showed limited colocalization with PHF within this period. In summary, we have developed a neuron-like model that clarifies the early intracellular dynamics of the interaction of tau antibodies with pathological tau, and identifies features associated with efficacy. Since the model is entirely human, it is suitable to verify the therapeutic potential of humanized antibodies prior to extensive clinical trials.
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http://dx.doi.org/10.3389/fneur.2020.602292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727311PMC
November 2020

Pancreatitis, Panniculitis, and Polyarthritis Syndrome Detected on 99mTc-MDP Bone Scan.

Clin Nucl Med 2021 Mar;46(3):261-263

Department of Pediatrics, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, People's Republic of China China.

Abstract: Pancreatitis, panniculitis, and polyarthritis syndrome is a rare disease. A 49-year-old man recently complained of bilateral ankle and epigastric pain. An ankle x-ray showed subcutaneous soft tissue swelling. Subsequent 99mTc-MDP bone scan showed foci of abnormal activity around joints in all extremities, especially in the knees and ankles. In addition, multiple nodular calcifications in the head of the pancreas were found in the abdominal CT scan. The patient also had increased blood amylase and lipase. The symptoms were resolved after the treatment of his pancreatitis.
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http://dx.doi.org/10.1097/RLU.0000000000003460DOI Listing
March 2021

Recent Progress in Black Phosphorus Sensors.

J Biomed Nanotechnol 2020 Jul;16(7):1045-1064

Black phosphorus (BP) is a new two-dimensional material with many unique properties, such as great biocompatibility, excellent surface activity, high carrier mobility, and tunable bandgap. Black phosphorus has been particularly attractive in sensor. However, black phosphorus isolated by traditional methods is easily oxidized and degraded by air, with poor stability, which limits its application as sensors. The modification and functionalization of black phosphorus enhance the stability, sensitivity, selectivity and biocompatibility of its application in sensor. This review describes recent progresses in sensor based on black phosphorus (2016-2020). Firstly, the structure and properties, synthesis methods, modification and functionalization of black phosphorus are briefly described. Then, the advancements in designing of various sensors based on black phosphorus are systematically provided, with a specific focus on electrochemical sensors, electrochemiluminescence sensors and photoelectrochemical sensors. Finally, latest challenges and further opportunities for developing new sensors with black phosphorus nanomaterial are discussed.
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http://dx.doi.org/10.1166/jbn.2020.2963DOI Listing
July 2020

Self-Assembly Based Cancer Therapy Strategy.

J Biomed Nanotechnol 2020 Jul;16(7):997-1017

Nanotechnology has been widely applied in tumor imaging, diagnostic and therapy. Beside the prefabricated nanomaterials, constructing nanostructures in living cells through self-assemble provides an alternative strategy to treat cancer. self-assembly renders the conversion of compatible small molecules into assembled nanostructures with toxicity, and is expected to outperform the prefabricated nanotechnologies as the small molecules diffuse faster than their assembly form. Attributed to the specific tumor environment such as low pH, high ROS, high enzyme expression and so on, self-assembly could differentiate cancer cells from normal ones with high selectivity. The self-assembly based caner therapy has made considerable progress in the last decade with confirmed advantages such as high capacity, minimal drug resistance, high accumulation, enhanced retention and so on. This review summarized the self-assembly of nanostructures induced by the stimuli like pH, ROS, enzyme, metal ion, localized concentration, biominerization and their utilization in cancer therapy.
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http://dx.doi.org/10.1166/jbn.2020.2962DOI Listing
July 2020

Supramolecular Adhesion at Extremely Low Temperatures: A Combined Experimental and Theoretical Investigation.

J Am Chem Soc 2020 12 10;142(51):21522-21529. Epub 2020 Dec 10.

College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, P. R. China.

Adhesive materials that are resistant to low temperatures have wide applications in daily life, scientific research, and industry. Currently, the overwhelming majority of low-temperature-resistant adhesives are traditional polymer systems. In this study, a new strategy was developed to obtain strong and long-lasting adhesion effects from low-molecular-weight adhesives at low temperatures. The introduction of water molecules and the formation of hydrogen bonds not only triggered supramolecular polymerization but also endowed the water-involved copolymer with low-temperature resistance. The water content of the polymeric supramolecular system played a crucial role in exhibiting adhesion behavior at low temperatures. Good adhesion performance was obtained in extremely low-temperature environments, including liquid nitrogen.
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http://dx.doi.org/10.1021/jacs.0c10786DOI Listing
December 2020