Publications by authors named "Yan Cheng"

1,336 Publications

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Heterostructured CoS/[email protected] carbon hollow sphere for potassium-ion batteries.

J Colloid Interface Sci 2021 Sep 29;608(Pt 1):275-283. Epub 2021 Sep 29.

Institute of Advanced Structure Technology, Beijing Institute of Technology, Beijing 100081, China. Electronic address:

Potassium ions batteries (PIBs) have been regarded as a promising choice for electrical energy storage technology due to the wide distribution of potassium resources. However, developing low-cost and robust earth-rich anode materials is still a major challenge for the practical and scalable usage of PIBs. Herein, for the first time, we developed nitrogen doped carbon coating CoS/CuCoS heterostructure (CoS/[email protected]) hollow spheres and evaluated as anode for PIBs. The CoS and CuCoS heterostructure interface could generate a built-in electric field, which can fasten electrons transportation. The nanostructures could shorten the diffusion length of K and provide large surface area to contact with electrolytes. Furthermore, the inner hollow sphere morphology along with the carbon layer could accommodate the volume expansion during cycling. What's more, the N-doped carbon could increase the conductivity of the anodes. Benefitting from the above features, the CoS/[email protected] displays an outstanding rate capability (309 mAh g at 500 mA g after 250 cycles) and a long-term cycling life (112 mAh g at 1000 mA g after 1000 cycles) in ether-based electrolyte. Conversion reaction mechanism in CoS/[email protected] anode is also revealed through ex situ XRD characterizations. This work provides a practical direction for investigating metal sulfides as anode for PIBs.
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http://dx.doi.org/10.1016/j.jcis.2021.09.137DOI Listing
September 2021

Time-restricted feeding improves blood glucose and insulin sensitivity in overweight patients with type 2 diabetes: a randomised controlled trial.

Nutr Metab (Lond) 2021 Oct 7;18(1):88. Epub 2021 Oct 7.

NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, China.

Background: Time-restricted feeding is an emerging dietary intervention that is becoming increasingly popular. There are, however, no randomised clinical trials of time-restricted feeding in overweight patients with type 2 diabetes. Here, we explored the effects of time-restricted feeding on glycaemic regulation and weight changes in overweight patients with type 2 diabetes over 12 weeks.

Methods: Overweight adults with type 2 diabetes (n = 120) were randomised 1:1 to two diet groups: time-restricted feeding (n = 60) or control (n = 60). Sixty patients participated in a 10-h restricted feeding treatment program (ad libitum feeding from 8:00 to 18:00 h; fasting between 18:00 and 8:00 h) for 12 weeks.

Results: Haemoglobin A1c and body weight decreased in the time-restricted feeding group (- 1.54% ± 0.19 and - 2.98 ± 0.43 kg, respectively) relative to the control group over 12 weeks (p < 0.001). Homeostatic model assessment of β-cell function and insulin resistance changed in the time-restricted feeding group (0.73 ± 0.21, p = 0.005; - 0.51 ± 0.08, p = 0.02, respectively) compared with the control group. The medication effect score, SF-12 score, and the levels of triglycerides, total cholesterol and low-density lipoprotein cholesterol were improved in the time-restricted feeding group (- 0.66 ± 0.17, p = 0.006; 5.92 ± 1.38, p < 0.001; - 0.23 ± 0.08 mmol/L, p = 0.03; - 0.32 ± 0.07 mmol/L, p = 0.01; - 0.42 ± 0.13 mmol/L, p = 0.02, respectively) relative to the control group. High-density lipoprotein cholesterol was not significantly different between the two groups.

Conclusion: These results suggest that 10-h restricted feeding improves blood glucose and insulin sensitivity, results in weight loss, reduces the necessary dosage of hypoglycaemic drugs and enhances quality of life. It can also offer cardiovascular benefits by reducing atherosclerotic lipid levels.

Trial Registration: This study was registered with the Chinese Clinical Trial Registry (ChiCTR-IPR-15006371).
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http://dx.doi.org/10.1186/s12986-021-00613-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8499480PMC
October 2021

Enhancing Cell Performance of Lithium-Rich Manganese-Based Materials via Tailoring Crystalline States of a Coating Layer.

ACS Appl Mater Interfaces 2021 Oct 5;13(41):49390-49401. Epub 2021 Oct 5.

School of Metallurgy and Environment, Central South University, Changsha, Hunan 410083, China.

Li-rich Mn-based-layered oxides are considered to be the most felicitous cathode material candidates for commercial application of lithium-ion batteries on account of high energy density. Nevertheless, defects containing an unsatisfactory initial Coulombic efficiency and rapid voltage decay seriously impede their practical utilization. Herein, a coating layer with three distinct crystalline states are employed as a coating layer to modify Li[LiMnNiCo]O, respectively, and the effects of coating layers with distinct crystalline states on the crystal structure, diffusion kinetics, and cell performance of host materials are further explored. A coating layer with high crystallinity enables mitigatory voltage decay and better cyclic stability of materials, while a coating layer with planar defects facilitates Li transfer and enhances the rate performance of materials. Consequently, optimizing the crystalline state of coating substances is critical for preferable surface modification.
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http://dx.doi.org/10.1021/acsami.1c11180DOI Listing
October 2021

Outcomes of routine screening for reproductive coercion in a family planning service.

Sex Health 2021 Oct 5. Epub 2021 Oct 5.

Background: Reproductive coercion (RC) occurs when a person's autonomous decision-making regarding reproductive health is compromised by another. RC screening, that is, the use of routine, non-invasive screening questions asked of service users/clients, is one strategy that can be used to assess for RC. Routine screening for RC was implemented within Family Planning NSW clinical consultations in December 2018. A cross-sectional study was undertaken to review the outcomes of screening to better understand the situation of RC among women accessing family planning services.

Methods: A retrospective review of clinical consultation data of eligible women attending Family Planning NSW clinics in 2019 was undertaken. Descriptive analysis was conducted and modified Poisson regression was used to estimate prevalence ratios and assess associations between binary outcomes and client characteristics.

Results: Of 7943 women eligible for RC screening, 5497 were screened (69%) and 127 women (2.3%) disclosed RC. RC was more likely to be disclosed among clients who were unemployed, had a disability or had more than one visit within 1 year.

Conclusions: Sexual and reproductive health clinicians, in particular, are well placed to conduct RC screening. However, they must have adequate training and access to resources to implement screening and respond to women who disclose RC.
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http://dx.doi.org/10.1071/SH21079DOI Listing
October 2021

Copper-doping defect-lowered perovskite nanosheets for deep-blue light-emitting diodes.

J Colloid Interface Sci 2021 Sep 15;607(Pt 2):1796-1804. Epub 2021 Sep 15.

Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, State Key Laboratory of Traction Power, Southwest Jiaotong University, Chengdu 610031, PR China. Electronic address:

Mixed-halide blue perovskites CsPb(Br/Cl) are considered promising candidates for developing efficient deep-blue perovskite light-emitting diodes (PeLEDs), but their low photoluminescence quantum yield (PLQY), environmental instability, and poor device performance gravely inhibit their future development. Here, we employ a heteroatomic Cu doping strategy combined with post-treatment Br anion exchange to prepare high-performance deep-blue perovskites CsPb(Br/Cl). The Cu doping strategy significantly decreases the intrinsic chlorine defects, ensuring that the inferior CsPbCl quantum dots are transformed into two-dimensional nanosheets with enhanced violet photoluminescence and increased exciton binding energy. Further, with the post-treatment Br anion exchange, the as-prepared CsPb(Br/Cl) nanosheets with more radiation recombination and less ion migration present an enhanced PLQY of 94% and better humidity stability of 30 days. Based on the optimized CsPb(Br/Cl), we fabricated deep-blue PeLEDs with luminescence emission at 462 nm, a maximum luminance of 761 cd m, and a current density of 205 mA cm. This work puts forward a feasible synthesis strategy to prepare efficient and stable mixed-halide blue perovskite CsPb(Br/Cl) and related blue PeLEDs, which may promote the further application of mixed-halide perovskites in the blue light range.
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http://dx.doi.org/10.1016/j.jcis.2021.09.061DOI Listing
September 2021

Estimating acceptable exposure time for bioaerosols emission in a wastewater treatment plant by reverse quantitative microbial risk assessment based on various risk benchmarks.

Environ Sci Pollut Res Int 2021 Sep 29. Epub 2021 Sep 29.

School of Environmental Studies, China University of Geosciences, 388 Lumo Road, Wuhan, 430074, People's Republic of China.

Populations exposed to bioaerosols over time in wastewater treatment plants (WWTPs) will be infected. Then, the reverse quantitative microbial risk assessment (QMRA) provides a quantitative framework for the estimation of acceptable exposure time to protect people from excessive exposure and then manage their health risk. In this study, the acceptable exposure time for staffs and visiting researchers exposed to S. aureus or E. coli bioaerosols emitted from aeration ponds in WWTPs was estimated and analyzed by Monte Carlo simulation-based reverse QMRA (using the 1E-4 pppy suggested by the US EPA or 1E-6 DALYs pppy suggested by the WHO as benchmarks). The 1E-3 and 1E-2 pppy were selected as a series of loose annual infection risk benchmarks to calculate a practical acceptable exposure time. The results showed that for the acceptable exposure time in each specific exposure scenario, the exposure of females was consistently 0.3-0.4 times longer than that of males; the exposure of staffs was 3.6-3.9 times shorter than that of visiting researchers; the exposures of populations in the rotating-disc aeration mode were consistently 6.3-6.6 and 2.8-3.1 times longer than those in the microporous aeration mode for S. aureus and E. coli bioaerosols, respectively. The acceptable exposure time with the use of personal protective equipment (PPE) was 33.4-35.0 times as long as that without PPE. The US EPA benchmark is stricter than the WHO benchmark with regard to the estimation of the acceptable exposure time of S. aureus or E. coli bioaerosols. The 1E-3 pppy is more appropriate and practical than the US EPA benchmark, but the 1E-2 pppy is notably too loose for health risk management. This research can assist managers of WWTPs to formulate a justified exposure time and develop applicable administrative and personal intervention strategies. The results can enrich the knowledge bases of reverse QMRA to elect a series of loose health-based target risk benchmarks for health risk management.
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http://dx.doi.org/10.1007/s11356-021-16699-wDOI Listing
September 2021

Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction.

Acta Pharmacol Sin 2021 Sep 29. Epub 2021 Sep 29.

Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.

Physapubenolide (PB), a withanolide-type compound extracted from the traditional herb Physalis minima L., has been demonstrated to exert remarkable cytotoxicity against cancer cells; however, its molecular mechanisms are still unclear. In this study, we demonstrated that PB inhibited cell proliferation and migration in melanoma cells by inducing cell apoptosis. The anticancer activity of PB was further verified in a melanoma xenograft model. To explore the mechanism underlying the anticancer effects of PB, we carried out an in silico target prediction study, which combined three approaches (chemical similarity searching, quantitative structure-activity relationship (QSAR), and molecular docking) to identify the targets of PB, and found that PB likely targets 3-hydroxy-methylglutaryl CoA reductase (HMGCR), the rate-limiting enzyme of the mevalonate pathway, which promotes cancer cell proliferation, migration, and metastasis. We further demonstrated that PB interacted with HMGCR, decreased its protein expression and inhibited the HMGCR/YAP pathway in melanoma cells. In addition, we found that PB could restore vemurafenib sensitivity in vemurafenib-resistant A-375 cells, which was correlated with the downregulation of HMGCR. In conclusion, we demonstrate that PB elicits anticancer action and enhances sensitivity to vemurafenib by targeting HMGCR.
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http://dx.doi.org/10.1038/s41401-021-00745-xDOI Listing
September 2021

AKAP9 supports spermatogenesis through its effects on microtubule and actin cytoskeletons in the rat testis.

FASEB J 2021 10;35(10):e21925

The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China.

In mammalian testes, extensive remodeling of the microtubule (MT) and actin cytoskeletons takes place in Sertoli cells across the seminiferous epithelium to support spermatogenesis. However, the mechanism(s) involving regulatory and signaling proteins remains poorly understood. Herein, A-kinase anchoring protein 9 (AKAP9, a member of the AKAP multivalent scaffold protein family) was shown to be one of these crucial regulatory proteins in the rat testis. Earlier studies have shown that AKAP9 serves as a signaling platform by recruiting multiple signaling and regulatory proteins to create a large protein complex that binds to the Golgi and centrosome to facilitate the assembly of the MT-nucleating γ-tubulin ring complex to initiate MT polymerization. We further expanded our earlier studies based on a Sertoli cell-specific AKAP9 knockout mouse model to probe the function of AKAP9 by using the techniques of immunofluorescence analysis, RNA interference (RNAi), and biochemical assays on an in vitro primary Sertoli cell culture model, and an adjudin-based animal model. AKAP9 robustly expressed across the seminiferous epithelium in adult rat testes, colocalizing with MT-based tracks, and laid perpendicular across the seminiferous epithelium, and prominently expressed at the Sertoli-spermatid cell-cell anchoring junction (called apical ectoplasmic specialization [ES]) and at the Sertoli cell-cell interface (called basal ES, which together with tight junction [TJ] created the blood-testis barrier [BTB]) stage specifically. AKAP9 knockdown in Sertoli cells by RNAi was found to perturb the TJ-permeability barrier through disruptive changes in the distribution of BTB-associated proteins at the Sertoli cell cortical zone, mediated by a considerable loss of ability to induce both MT polymerization and actin filament bundling. A considerable decline in AKAP9 expression and a disruptive distribution of AKAP9 across the seminiferous tubules was also noted during adjudin-induced germ cell (GC) exfoliation in this animal model, illustrating AKAP9 is essential to maintain the homeostasis of cytoskeletons to maintain Sertoli and GC adhesion in the testis.
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http://dx.doi.org/10.1096/fj.202100960RDOI Listing
October 2021

Genome-Wide Investigation of Family Genes in Pineapple and Functional Analysis of AcoSBT1.12 in Floral Transition.

Front Genet 2021 7;12:730821. Epub 2021 Sep 7.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Sciences, College of Plant Protection, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China.

SBT (Subtilisin-like serine protease), a clan of serine proteolytic enzymes, plays a versatile role in plant growth and defense. Although family genes have been obtained from studies of dicots such as Arabidopsis, little is known about the potential functions of SBT in the monocots. In this study, 54 pineapple genes (s) were divided into six subfamilies and then identified to be experienced strong purifying selective pressure and distributed on 25 chromosomes unevenly. -acting element analysis indicated that almost all s promoters contain light-responsive elements. Further, the expression pattern via RNA-seq data showed that different s were preferentially expressed in different above-ground tissues. Transient expression in tobacco showed that AcoSBT1.12 was located in the plasma membrane. Moreover, Transgenic Arabidopsis ectopically overexpressing exhibited delayed flowering time. In addition, under the guidance of bioinformatic prediction, we found that AcoSBT1.12 could interact with AcoCWF19L, AcoPUF2, AcoCwfJL, Aco012905, and AcoSZF1 by yeast-two hybrid (Y2H). In summary, this study provided valuable information on pineapple genes and illuminated the biological function of in floral transition.
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http://dx.doi.org/10.3389/fgene.2021.730821DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452990PMC
September 2021

Electrochemical Microfluidic Paper-Based Aptasensor Platform Based on a Biotin-Streptavidin System for Label-Free Detection of Biomarkers.

ACS Appl Mater Interfaces 2021 Oct 21;13(39):46317-46324. Epub 2021 Sep 21.

State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, P. R. China.

Timely and rapid detection of biomarkers is extremely important for the diagnosis and treatment of diseases. However, going to the hospital to test biomarkers is the most common way. People need to spend a lot of money and time on various tests for potential disease detection. To make the detection more convenient and affordable, we propose a paper-based aptasensor platform in this work. This device is based on a cellulose paper, on which a three-electrode system and microfluidic channels are fabricated. Meanwhile, novel nanomaterials consisting of amino redox graphene/thionine/streptavidin-modified gold nanoparticles/chitosan are synthesized and modified on the working electrode of the device. Through the biotin-streptavidin system, the aptamer whose 5'end is modified with biotin can be firmly immobilized on the electrode. The detection principle is that the current generated by the nanomaterials decreases proportionally to the concentration of targets owing to the combination of the biomarker and its aptamer. 17β-Estradiol (17β-E2), as one of the widely used diagnostic biomarkers of various clinical conditions, is adopted for verifying the performance of the platform. The experimental results demonstrated that this device enables the determination of 17β-E2 in a wide linear range of concentrations of 10 pg mL to 100 ng mL and the limit of detection is 10 pg mL (S/N = 3). Moreover, it enables the detection of targets in clinical serum samples, demonstrating its potential to be a disposable and convenient integrated platform for detecting various biomarkers.
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http://dx.doi.org/10.1021/acsami.1c12716DOI Listing
October 2021

Efficacy of Huaiqihuang granules as adjuvant therapy for bronchial asthma in children: a real-world study.

Zhongguo Dang Dai Er Ke Za Zhi 2021 Sept 15;23(9):877-881

Children's Hospital of Capital Institute of Pediatrics, Beijing 100020, China(LiuC-H, Email:

Objectives: To study the efficacy of Huaiqihuang granules as adjuvant therapy for bronchial asthma in children.

Methods: A multicenter, prospective, and registered real-world study was performed for the children, aged 2-5 years, who had a confirmed diagnosis of bronchial asthma in the outpatient service of 21 hospitals in China. Among these children, the children treated with medications for long-term asthma control (inhaled corticosteroid and/or leukotriene receptor antagonist) without Huaiqihuang granules were enrolled as the control treatment group, and those treated with medications for long-term asthma control combined with Huaiqihuang granules were enrolled as the combined treatment group. The medical data of all children were collected. Outpatient or telephone follow-up was performed at weeks 4, 8, 12, 20, 28, and 36 after treatment, including asthma attacks and rhinitis symptoms. A statistical analysis was performed for the changes in these indices.

Results: There was no significant difference in the frequency of asthma attacks or rhinitis attacks between the two groups before treatment (>0.05). After treatment, the combined treatment group had significantly lower frequencies of asthma attacks, severe asthma attacks, and rhinitis attacks compared with the control treatment group (<0.05). There was no signification difference in the incidence rate of adverse reactions between the two groups (=0.667).

Conclusions: Huaiqihuang granules in addition to medications for long-term asthma control can alleviate the symptoms of bronchial asthma and rhinitis and improve the level of asthma control in children with bronchial asthma, with good safety and little adverse effect. .
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http://dx.doi.org/10.7499/j.issn.1008-8830.2106028DOI Listing
September 2021

Understanding the Effect of Al Doping on the Electrochemical Performance Improvement of the LiMnO Cathode Material.

ACS Appl Mater Interfaces 2021 Sep 17;13(38):45446-45454. Epub 2021 Sep 17.

Key Laboratory of Polar Materials and Devices (MOE) and Department of Electronics Sciences, School of Physics and Electronic Science, East China Normal University, Shanghai 200062, China.

It is well known that the electrochemical performance of spinel LiMnO can be improved by Al doping. Herein, combining X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, and spherical aberration-corrected scanning transmission electron microscopy (Cs-STEM) with electron-beam (E-beam) irradiation techniques, the influence of Al doping on the structural evolution and stability improvement of the LiMnO cathode material is revealed. It is revealed that an appropriate concentration of Al ions could dope into the spinel structure to form a more stable LiAlMnO phase framework, which can effectively stabilize the surface and bulk structure by inhibiting the dissolution of Mn ions during cycling. The optimized LiAlMnO sample exhibits a superior capacity retention ratio of 80% after 1000 cycles at 10 C (1 C = 148 mA h g) in the voltage range of 3.0-4.5 V, which possesses an initial discharge capacity of 90.3 mA h g. Compared with the undoped LiMnO sample, the Al-doped sample also shows superior rate performance, especially the capacity recovery performance.
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http://dx.doi.org/10.1021/acsami.1c11315DOI Listing
September 2021

The Downregulation of eIF3a Contributes to Vemurafenib Resistance in Melanoma by Activating ERK via PPP2R1B.

Front Pharmacol 2021 27;12:720619. Epub 2021 Aug 27.

Department of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, and National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.

Vemurafenib, a BRAF V600E inhibitor, provides therapeutic benefits for patients with melanoma, but the frequent emergence of drug resistance remains a challenge. An understanding of the mechanisms underlying vemurafenib resistance may generate novel therapeutic strategies for patients with melanoma. Here, we showed that eIF3a, a translational regulatory protein, was an important mediator involved in vemurafenib resistance. eIF3a was expressed at significantly lower levels in vemurafenib-resistant A375 melanoma cells (A375R) than in parental A375 cells. Overexpression of eIF3a enhanced the sensitivity to BRAF inhibitors by reducing p-ERK levels Furthermore, eIF3a controlled ERK activity by regulating the expression of the phosphatase PPP2R1B via a translation mechanism, thus determining the sensitivity of melanoma cells to vemurafenib. In addition, a positive correlation between eIF3a and PPP2R1B expression was also observed in tumor samples from the Human Protein Atlas and TCGA databases. In conclusion, our studies reveal a previously unknown molecular mechanism of BRAF inhibitor resistance, which may provide a new strategy for predicting vemurafenib responses in clinical treatment.
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http://dx.doi.org/10.3389/fphar.2021.720619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430041PMC
August 2021

Biomarkers for the prediction of esophageal cancer neoadjuvant chemoradiotherapy response: A systemic review.

Crit Rev Oncol Hematol 2021 Sep 8;167:103466. Epub 2021 Sep 8.

Department of Radiation Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Neoadjuvant chemoradiotherapy followed by surgery has been established as the standard treatment for locally advanced esophageal cancer. For patients with complete regression after neoadjuvant chemotherapy, active surveillance rather than planned surgery has been proposed as an organ preservation strategy. Reliable biomarkers to predict chemoradiation response is needed. We first summarized the previous reports of biomarkers with the potential to predict the treatment response of esophageal cancer neoadjuvant chemoradiotherapy. These traditional biomarkers are classified into three groups: genetic biomarkers, RNA biomarkers, and protein biomarkers. We then summarized some special types of biomarkers, including metabolites biomarkers, immune and tumor microenvironment biomarkers, and microbiome biomarkers.
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http://dx.doi.org/10.1016/j.critrevonc.2021.103466DOI Listing
September 2021

Blocking antibody-mediated phosphatidylserine enhances cancer immunotherapy.

J Cancer Res Clin Oncol 2021 Sep 9. Epub 2021 Sep 9.

Department of General Surgery, Institute of General Surgery, Northern Jiangsu Province Hospital, Clinical Medical College, Yangzhou University, Yangzhou, 225001, People's Republic of China.

Cancer immunotherapy is a major breakthrough in tumor therapy and has been used in monotherapy or combination therapy. However, it has been associated with poor immune tolerance in some patients or immune-related adverse events. Therefore, ideal and reliable tumor elimination strategies are urgently needed to overcome these shortcomings. Phosphatidylserine (PS) is a negatively charged phospholipid, usually present in the inner lobules of eukaryotic cell membranes. Under certain physiological or pathological conditions, PS may be exposed on the outer leaflets of apoptotic cells serving as recognition signals by phagocytes and modulating the immune response. On the contrary, increased exposure of PS in the tumor microenvironment can significantly antagonize the body's anti-tumor immunity, thereby promoting tumor growth and metastasis. During radiotherapy and chemotherapy, PS-mediated immunosuppression increases the PS levels in necrotic tissue in the tumor microenvironment, further suppressing tumor immunity. PS-targeted therapy is a promising strategy in cancer immunotherapy. It inhibits tumor growth and improves the anti-tumor activity of immune checkpoint inhibitors. A comprehensive understanding of the mechanism of PS-targeted therapy opens up a new perspective for future cancer immunotherapies.
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http://dx.doi.org/10.1007/s00432-021-03792-3DOI Listing
September 2021

Metabolomics reveals the role of isopentenyl group in coumarins metabolism.

Biomed Chromatogr 2021 Sep 7:e5239. Epub 2021 Sep 7.

Laboratory of Metabolomics and Drug-Induced Liver Injury, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.

Coumarins are a group of natural compounds commonly found in the families of Rutaceae and Umbelliferae. 7-Isopentenyloxycoumarin (ISC), auraptene (AUR), and umbelliprenin (UM) belong to prenyloxycoumarins (PYCs), which link isopentenyl, geranyl, and farnesyl group at C7 position, respectively. The substituent of 7-ethoxycoumarin (ETC) is the ethyl group. In this study, UPLC-ESI-QTOF-MS (ultra-performance liquid chromatography-electrospray ionization-quadrupole time of flight-MS)-based metabolomics was used to evaluate the in vivo and in vitro metabolism of PYCs. Results showed that ETC produced 10 known metabolites, and ISC was transformed into 17 metabolites in vivo and in vitro, which were undescribed compounds. A total of 35 AUR metabolites, including 34 undescribed metabolites were identified, and 21 metabolites were reported for the first time in UM. The results indicated that hydroxylation and N-acetylcysteine conjugation were the common metabolic reactions for PYCs. The metabolic rates of ETC, ISC, AUR and UM were 26%, 36%, 81%, and 38%, respectively, in human liver microsome, while they were 24%, 40%, 80%, and 37%, respectively, in mouse liver microsomes. In addition, recombinant cytochrome P450s (CYPs) screening showed that CYP1A1, 2C19, 3A4, and 3A5 were the major metabolic enzymes involved in the formation of hydroxylation metabolites. Together, these results suggest that the isopentenyl group plays an important role in the metabolism of PYCs.
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http://dx.doi.org/10.1002/bmc.5239DOI Listing
September 2021

Surface treatment of 3D printed porous Ti6Al4V implants by ultraviolet photofunctionalization for improved osseointegration.

Bioact Mater 2022 Jan 25;7:26-38. Epub 2021 Jun 25.

Department of Orthopedics, Peking University Third Hospital, Beijing, 100191, People's Republic of China.

Three-dimensional (3D)-printed porous Ti6Al4V implants play an important role in the reconstruction of bone defects. However, its osseointegration capacity needs to be further improved, and related methods are inadequate, especially lacking customized surface treatment technology. Consequently, we aimed to design an omnidirectional radiator based on ultraviolet (UV) photofunctionalization for the surface treatment of 3D-printed porous Ti6Al4V implants, and studied its osseointegration promotion effects and , while elucidating related mechanisms. Following UV treatment, the porous Ti6Al4V scaffolds exhibited significantly improved hydrophilicity, cytocompatibility, and alkaline phosphatase activity, while preserving their original mechanical properties. The increased osteointegration strength was further proven using a rabbit condyle defect model , in which UV treatment exhibited a high efficiency in the osteointegration enhancement of porous Ti6Al4V scaffolds by increasing bone ingrowth (BI), the bone-implant contact ratio (BICR), and the mineralized/osteoid bone ratio. The advantages of UV treatment for 3D-printed porous Ti6Al4V implants using the omnidirectional radiator in the study were as follows: 1) it can significantly improve the osseointegration capacity of porous titanium implants despite the blocking out of UV rays by the porous structure; 2) it can evenly treat the surface of porous implants while preserving their original topography or other morphological features; and 3) it is an easy-to-operate low-cost process, making it worthy of wide clinical application.
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http://dx.doi.org/10.1016/j.bioactmat.2021.05.043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377410PMC
January 2022

Testis Toxicants: Lesson from Traditional Chinese Medicine (TCM).

Adv Exp Med Biol 2021 ;1288:307-319

The Mary M. Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, New York, NY, USA.

The testis is one of the organs in the mammalian body that is sensitive to toxicants. Accumulating evidence has shown that human exposure to toxic ingredients in Traditional Chinese Medicine (TCM), such as triptolide, gossypol, cannabidol, piperine, α-solanine, matrine, aristolochic acid, and emodin, lead to testis injury and reproductive dysfunction. The most obvious phenotype is reduced sperm counts due to defects in spermatogenesis. Studies have also shown that Sertoli cells in the seminiferous tubule, the functional unit of the testis that supports spermatogenesis, are the cell type that is most sensitive to the disruptive effects of toxicants. Since Sertoli cells are the "mother cells" that nurture germ cell development, Sertoli cell injury thus leads to failure in germ cell development in the seminiferous epithelium. Mounting evidence has shown that the Sertoli cell cytoskeletons, mitochondria function, Leydig cells steroidogenesis pathways and sperm ion channels are some of the prime targets of toxicants from TCM. We carefully evaluate recent findings in this area of research herein, and to provide a summary of these findings, including some insightful information regarding the underlying molecular basis of toxicant-induced testis injury that impede spermatogenesis.
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http://dx.doi.org/10.1007/978-3-030-77779-1_15DOI Listing
August 2021

Spermiation: Insights from Studies on the Adjudin Model.

Adv Exp Med Biol 2021 ;1288:241-254

Sir Run Run Shaw Hospital (SRRSH), Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Spermatogenesis is comprised of a series of cellular events that lead to the generation of haploid sperm. These events include self-renewal of spermatogonial stem cells (SSC), proliferation of spermatogonia by mitosis, differentiation of spermatogonia and spermatocytes, generation of haploid spermatids via meiosis I/II, and spermiogenesis. Spermiogenesis consists of a series of morphological events in which spermatids are being transported across the apical compartment of the seminiferous epithelium while maturing into spermatozoa, which include condensation of the genetic materials, biogenesis of acrosome, packaging of the mitocondria into the mid-piece, and elongation of the sperm tail. However, the biology of spermiation remains poorly understood. In this review, we provide in-depth analysis based on the use of bioinformatics tools and an animal model that mimics spermiation through treatment of adult rats with adjudin, a non-hormonal male contraceptive known to induce extensive germ cell exfoliation across the seminiferous epithelium, but nost notably elongating/elongated spermatids. These analyses have shed insightful information regaridng the biology of spermiation.
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http://dx.doi.org/10.1007/978-3-030-77779-1_12DOI Listing
August 2021

Male Infertility in Humans: An Update on Non-obstructive Azoospermia (NOA) and Obstructive Azoospermia (OA).

Adv Exp Med Biol 2021 ;1288:161-173

Sir Run Run Shaw Hospital (SRRSH), Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Non-obstructive azoospermia (NOA) and obstructive azoospermia (OA) are two common causes of infertility that affect a considerable number of men. However, few studies were performed to understand the molecular etiology of these disorders. Studies based on bioinformatics and genetic analyses in recent years, however, have yielded insightful information and have identified a number of genes that are involved in these disorders. In this review, we briefly summarize and evaluate these findings. We also discuss findings based on epigenetic modifications of sperm DNAs that affect a number of genes pertinent to NOA and OA. The information summarized in this Chapter should be helpful to investigators in future functional studies of NOA and OA.
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http://dx.doi.org/10.1007/978-3-030-77779-1_8DOI Listing
August 2021

Motor Proteins and Spermatogenesis.

Adv Exp Med Biol 2021 ;1288:131-159

Sir Run Run Shaw Hospital (SRRSH), Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Unlike the intermediate filament- and septin-based cytoskeletons which are apolar structures, the microtubule (MT) and actin cytoskeletons are polarized structures in mammalian cells and tissues including the testis, most notable in Sertoli cells. In the testis, these cytoskeletons that stretch across the epithelium of seminiferous tubules and lay perpendicular to the basement membrane of tunica propria serve as tracks for corresponding motor proteins to support cellular cargo transport. These cargoes include residual bodies, phagosomes, endocytic vesicles and most notably developing spermatocytes and haploid spermatids which lack the ultrastructures of motile cells (e.g., lamellipodia, filopodia). As such, these developing germ cells require the corresponding motor proteins to facilitate their transport across the seminiferous epithelium during the epithelial cycle of spermatogenesis. Due to the polarized natures of these cytoskeletons with distinctive plus (+) and minus (-) end, directional cargo transport can take place based on the use of corresponding actin- or MT-based motor proteins. These include the MT-based minus (-) end directed motor proteins: dyneins, and the plus (+) end directed motor proteins: kinesins, as well as the actin-based motor proteins: myosins, many of which are plus (+) end directed but a few are also minus (-) end directed motor proteins. Recent studies have shown that these motor proteins are essential to support spermatogenesis. In this review, we briefly summarize and evaluate these recent findings so that this information will serve as a helpful guide for future studies and for planning functional experiments to better understand their role mechanistically in supporting spermatogenesis.
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http://dx.doi.org/10.1007/978-3-030-77779-1_7DOI Listing
August 2021

Unraveling the Regulation of Cancer/Testis Antigens in Tumorigenesis Through an Analysis of Normal Germ Cell Development in Rodents.

Adv Exp Med Biol 2021 ;1288:69-93

Sir Run Run Shaw Hospital (SRRSH), Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Cancer/testis (CT) antigens are proteins aberrantly overexpressed in various tumorigenic cells, but they can also be normally expressed in the mammalian germline. Most CT antigens are highly immunogenic and known to be involved in cancer cell proliferation and tumor metastasis. A recent genome-wide analysis systematically identified CT antigen expression in 19 cancer types, significantly expanding the repertoire of CT antigens by 5-fold, from over 200 to approximately 1000. However, their function and regulation in tumorigenesis remain poorly understood. The shared functional characteristics between germ cells and cancer cells, if methodically defined, offer a unique gateway to understanding the regulation of CT antigens in cancers by studying gametogenesis. Nonetheless, such studies also provide insightful information on the role of CT antigens in spermatogenesis. Herein, we analyzed publicly available next generation sequencing datasets generated from normal adult testes in rodents, primordial germ cells and cancer samples across a series of published studies and databases. Based on these analyses, we report that a subset of CT antigens belonged to the core fitness gene family. Furthermore, super-enhancers both in normal testes and various cancers controlled specific CT antigens. We found that DNA methylation of CT antigens, such as TEX101 and TAF7L, was inversely correlated with their expression in both normal primordial germ cells and various cancers, which was mediated at least partly by DNA methyltransferase1 (DNMT1). By analyzing data from a testis knockout model, we showed that TAF7L could further influence the expression of additional CT antigens, which also held true in tumors. These findings not only confirmed the previous notion that CT antigens regulate cancer dynamics, but also showed that understanding the regulation of CT antigens during gametogenesis can offer new insights for cancer research.
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http://dx.doi.org/10.1007/978-3-030-77779-1_4DOI Listing
August 2021

Inhibition of autophagy with Chloroquine enhanced apoptosis induced by 5-aminolevulinic acid-photodynamic therapy in secondary hyperparathyroidism primary cells and organoids.

Biomed Pharmacother 2021 Oct 16;142:111994. Epub 2021 Aug 16.

Department of General Surgery, the Third Xiangya Hospital, Central South University, Changsha, Hunan Province 410000, China. Electronic address:

Secondary hyperparathyroidism (SHPT), the most common complication in the later stage of chronic kidney disease (CKD), seriously affects quality of life and the survival time of patients. At present, the conventional drugs and surgical methods still cannot fully meet the needs of clinical treatment. It is quite significant to develop effective and minimally invasive treatment methods. 5-Aminolevulinic acid-mediated photodynamic therapy (5-ALA-PDT), an alternative treatment relying on light irradiation, photosensitizer, and oxygen to produce a series of cytotoxic effects on tissue, is a promising technique for treating SHPT. We have successfully cultivated SHPT primary cells and organoids, and further proved that the amount of 5-ALA transformed into protoporphyrin IX in a time- and concentration-dependent manner. Also, 5-ALA-PDT exerted a cytotoxic effect on both primary cells and organoids by the cell counting kit (CCK-8) assay. Mechanically, 5-ALA-PDT increased the number of autophagosomes, and autophagy- and apoptosis-related proteins were upregulated markedly by western-blotting. The autophagy inhibitor Chloroquine (CQ) significantly increased the proportion of apoptotic cells, while the autophagy inducer rapamycin decreased the inhibitory ability of 5-ALA-PDT in SHPT primary cells. In brief, 5-ALA-PDT exhibits a phototoxic effect on SHPT primary cells and organoids. Autophagy and apoptosis are involved in the mechanism, and autophagy plays a role in promoting survival and inhibiting apoptosis. Therefore, the use of autophagy inhibitors can increase the sensitivity of SHPT cells and organoids treated with 5-ALA-PDT.
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http://dx.doi.org/10.1016/j.biopha.2021.111994DOI Listing
October 2021

From Co-MOF to CoNi-MOF to Ni-MOF: A Facile Synthesis of 1D Micro-/Nanomaterials.

Inorg Chem 2021 Sep 19;60(17):13168-13176. Epub 2021 Aug 19.

School of Chemistry and Chemical Engineering, Institute for Innovative Materials and Energy, Yangzhou University, Yangzhou 225002, Jiangsu, P. R. China.

Controlling the growth of metal-organic frameworks (MOFs) at the micro-/nanoscopic scale will result in new physical properties and novel functions into the materials without changing the chemical identities and the characteristic features of the MOFs themselves. Herein, we report a facile approach to synthesize a series of MOFs [Co-MOF, CoNi-MOFs ( and represent the molar ratio of Co and Ni and / = 1:1, 1:5, 1:10, 1:15, and 1:20), and Ni-MOF] with a one-dimensional micro-/nanoscaled rod-like architecture. From Co-MOF to CoNi-MOFs to Ni-MOF, the diameters of the rods turn to be spindly with the increase of Ni content which will facilitate the supercapacitor performances. Interestingly, CoNi-MOF exhibits a highest specific capacity of 597 F g at 0.5 A g and excellent cycle performance (retained 93.59% after 4000 cycles) among these MOF materials owing to its micro-/nanorod structure with a smaller diameter and the synergy effect between the optimum molar ratio of Co and Ni.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01561DOI Listing
September 2021

Case report of the first molecular diagnosis of Stickler syndrome with a pathogenic COL2A1 variant in a Mongolia family.

Mol Genet Genomic Med 2021 Aug 18:e1781. Epub 2021 Aug 18.

Eye Center of the Second Hospital, Jilin University, Changchun, China.

Background: Stickler syndrome is a group of connective tissue disorders that can affect eye (myopia, cataract, and retinal detachment), skeleton (spondyloepiphyseal dysplasia and precocious arthritis), craniofacies (midfacial under development and cleft palate), and inner ear (conductive and sensorineural); with the degree of symptoms varying among patients. Mutations in the COL2A1, COL11A1, COL11A2, COL9A1, COL9A2, and COL9A3 procollagen genes cause Stickler syndrome.

Case Presentation: A 16-year-old Mongolian girl approached our clinics with retinal detachment. The proband had vitreous degeneration in both eyes, rhegmatogenous retinal detachment in her right eye, a large area of retina degeneration in her left eye, and coupled with severe myopia. No obvious hearing disorder was found, no abnormalities in bones and joints, and her communication and learning capability were also normal. Further clinical examination showed that the patient's other five family members across three generations had vitreous and retina degenerations. Exome sequencing showed a heterozygous splicing variant in COL2A1 in all patients.

Conclusions: In this case report, a pathogenic splicing variant in the COL2A1 gene was identified in a Mongolian family affected with Stickler syndrome type I by exome sequencing. This heterozygous splicing variant in COL2A1 (NM_001844.4:C.2518-1G>A) that may impair splicing, which was suggested by in silico prediction. Next-generation sequencing is helpful for the differential diagnosis of this clinically variable and genetically heterogeneous disorder.
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http://dx.doi.org/10.1002/mgg3.1781DOI Listing
August 2021

Novel concepts of molecular mechanisms in spermatogenesis.

Authors:
C Yan Cheng

Semin Cell Dev Biol 2021 Aug 11. Epub 2021 Aug 11.

Nantong University Medical School, Institute of Reproductive Medicine, Nantong, Jiangsu 226001, China. Electronic address:

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http://dx.doi.org/10.1016/j.semcdb.2021.08.005DOI Listing
August 2021

Tumor Inhibitory Effect of Long Non-coding RNA LOC100505817 on Gastric Cancer.

Pathol Oncol Res 2021 26;27:581542. Epub 2021 May 26.

Department of Oncology, The First Hospital of Qinhuangdao, Qinhuangdao, China.

Gastric cancer (GC) is one of the major malignancies worldwide. Emerging evidence has revealed the potential involvement of long noncoding RNA (lncRNA) in human genetic disorders and cancer, but the role of LOC100505817 remains unknown. Thus, in this study, we isolated tissues from GC patients to characterize the functional importance of LOC100505817 in GC tumorigenesis. We also proposed a hypothesis that the regulation of Wnt/β-catenin pathway by LOC100505817 was regulated by miR-20a-mediated WT1. After the collection of cancer tissues and adjacent tissues were obtained from GC patients, expression of LOC100505817, Wnt/β-catenin pathway- and EMT-related genes was quantified. Ectopic expression and knockdown experiments were applied in order to investigate the protective role of LOC100505817 in the progression of GC. Subsequently, cell viability, flow cytometry for apoptosis and cell cycle were detected via CCK-8, while migration and invasion were determined using scratch test and Transwell assay respectively. Then interactions among LOC100505817, miR-20a and WT1 were explored by dual luciferase reporter gene assay, RNA pull down assay and RNA binding protein immunoprecipitation (RIP) assay. The results found poor expression LOC100505817 was poorly expressed in GC cells and tissues. Overexpressed LOC100505817 resulted in the significant reduction of cell proliferation, migration and invasion as well as the expression of Wnt2b, β-catenin, CyclinD1, N-cadherin, Vimentin and snail, while increased cell apoptosis along with the expression of E-cadherin. Wnt/β-catenin pathway and EMT in GC cells were suppressed by LOC100505817 through miR-20a-inhibted WT1. In summary, our results provided evidence suggesting that LOC100505817 inhibits GC through LOC100505817-mediated inhibition of Wnt/β-catenin pathway, that leads to the overall restraining of GC cell proliferation, migration and invasion through miR-20a-reduced WT1.
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http://dx.doi.org/10.3389/pore.2021.581542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354317PMC
May 2021

Fat body Ire1 regulates lipid homeostasis through the Xbp1s-FoxO axis in .

iScience 2021 Aug 7;24(8):102819. Epub 2021 Jul 7.

Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences; the Institute for Advanced Studies, Wuhan University, Wuhan 430072, China.

The endoplasmic reticulum (ER)-resident transmembrane protein kinase/RNase Ire1 is a conserved sensor of the cellular unfolded protein response and has been implicated in lipid homeostasis, including lipid synthesis and transport, across species. Here we report a novel catabolic role of Ire1 in regulating lipid mobilization in . We found that Ire1 is activated by nutrient deprivation, and, importantly, fat body-specific deficiency leads to increased lipid mobilization and sensitizes flies to starvation, whereas fat body Ire1 overexpression results in the opposite phenotypes. Genetic interaction and biochemical analyses revealed that Ire1 regulates lipid mobilization by promoting Xbp1s-associated FoxO degradation and suppressing FoxO-dependent lipolytic programs. Our results demonstrate that Ire1 is a catabolic sensor and acts through the Xbp1s-FoxO axis to hamper the lipolytic response during chronic food deprivation. These findings offer new insights into the conserved Ire1 regulation of lipid homeostasis.
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http://dx.doi.org/10.1016/j.isci.2021.102819DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333185PMC
August 2021

Investor Sentiment and Stock Returns During the COVID-19 Pandemic.

Front Psychol 2021 20;12:708537. Epub 2021 Jul 20.

School of Economics, Zhejiang University of Technology, Zhejiang, China.

In this paper, we regard the Baidu index as an indicator of investors' attention to China's epidemic stocks. We believe that when seeking information to guide investment decisions, investor sentiment is usually affected by the information provided by the Baidu search engine, which may cause stock prices to fluctuate. Therefore, we constructed a GARCH extended model including the Baidu index to predict the return of epidemic stocks and compared it with the benchmark model. The empirical research in this paper finds that the forecast model including the Baidu index is significantly better than the benchmark model. This has important reference value both for investors in predicting stock trends and for the government's formulation of policies to prevent excessive stock market volatility.
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http://dx.doi.org/10.3389/fpsyg.2021.708537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329237PMC
July 2021
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