Publications by authors named "Yajuan Zhang"

74 Publications

Three-dimensional hydrated vanadium pentoxide/MXene composite for high-rate zinc-ion batteries.

J Colloid Interface Sci 2021 Mar 9;593:417-423. Epub 2021 Mar 9.

Shanghai Key Laboratory of Magnetic Resonance, School of Physics and Electronic Science, East China Normal University, Shanghai 200241, China. Electronic address:

Aqueous zinc-ion batteries (ZIBs) have been considered to be potential energy storage devices because of their cost-effectiveness and environmental friendliness. However, most of the cathode materials reported in ZIBs exhibit poor electrochemical performances like capacity fading during cycling and inferior performance at high current densities, which significantly hinder the further development of ZIBs. Here, we reported a novel three-dimensional hydrated vanadium pentoxide (VO·nHO)/MXene composite via a simple one-step hydrothermal method. Owing to the unique structure and high electrical conductivity of MXene, VO·nHO/TiCT MXene shows a remarkable electrochemical performance with a reversible capacity of 323 mAh g at 0.1 A g and exceptional rate capability (262 mAh g at 1 A g and 225 mAh g at 2 A g) when used as the cathode for aqueous ZIBs. This work offers a new insight into fabricating novel vanadium oxide-based cathode material for aqueous ZIBs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.02.090DOI Listing
March 2021

Effect of Repeated Anodal HD-tDCS on Executive Functions: Evidence From a Pilot and Single-Blinded fNIRS Study.

Front Hum Neurosci 2020 18;14:583730. Epub 2021 Jan 18.

School of Psychology, Shaanxi Normal University, Xi'an, China.

Executive functions are of vital importance in the process of active cognition, which is thought to be associated with the dorsolateral prefrontal cortex (DLPFC). As a valid brain stimulation technology, high-definition transcranial direct current stimulation (HD-tDCS) has been used to optimize cognitive function in healthy adults. Substantial evidence indicates that short-term or single anodal tDCS sessions over the left DLPFC will enhance the performance of executive functions. However, the changes in performance and cortical activation of executive functions after modulation by repeated anodal HD-tDCS is as yet unexplored. This study aims to examine changes in three core components of executive functions (inhibitory control, working memory, and cognitive flexibility) produced by nine HD-tDCS sessions (1.5 mA, over left DLPFC, 20 min per session), and to use functional near-infrared spectroscopy (fNIRS) to bilaterally record DLPFC neural activity. A total of 43 participants were divided randomly into two study groups (anodal group vs. sham group) to complete nine interventions. Our results demonstrate that the enhancement of cognitive flexibility in the anodal group was significantly better than that in the sham group. Additionally, a Stroop effect-related decrease in oxygenated hemoglobin (HbO) concentration in the DLPFC was observed in the anodal group but not the sham group. In conclusion, our study found that repeated anodal HD-tDCS sessions can significantly promote cognitive flexibility, one of the core components of executive function, and that alterations in DLPFC activation can enhance our understanding of the neuroplastic modifications modulated by HD-tDCS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnhum.2020.583730DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847848PMC
January 2021

Two novel BTD mutations causing profound biotinidase deficiency in a Chinese patient.

Mol Genet Genomic Med 2021 Jan 16:e1591. Epub 2021 Jan 16.

Medical Genetics Center, Southwest Hospital, Army Medical University, Chongqing, China.

Background: Biotinidase deficiency (OMIM 253260) is an autosomal recessively inherited disorder affecting about 1/60,000 people worldwide. The absence or deficiency of biotinidase impairs free biotin recycling and affects biotin-dependent carboxylase functions.

Methods: A Chinese patient with spontaneous recurrent epilepsy, an eczema-like rash, hair loss, hypotonia, and hearing loss began at three months of age. Her biotinidase activity was 1.0 nmol/ml/min, 9.5% of the mean control activity, which confirmed profound biotinidase deficiency.

Results: Compound heterozygous for c.250-1G > C and c.878dupT variants in the BTD gene were identified in this patient. These two variants were novel and absent in the population matched controls and any databases.

Conclusions: This study expanded the mutation spectrum of alterations of the BTD gene. Our patient also emphasized the critical role of biotinidase activity measurement combined with mutation analysis in early diagnosis of biotinidase deficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mgg3.1591DOI Listing
January 2021

Nitrogen and sulfur co-doped vanadium carbide MXene for highly reversible lithium-ion storage.

J Colloid Interface Sci 2021 Apr 17;587:489-498. Epub 2020 Dec 17.

Shanghai Key Laboratory of Magnetic Resonance, School of Physics and Electronic Science, East China Normal University, Shanghai 200241, China. Electronic address:

As an emerging group of two-dimensional (2D) layered material, MXenes have received significant attention in the direction of energy storage. However, the restacking of MXene flakes severely hinders the ion transport within electrodes, which limits their application for lithium-ion batteries (LIBs). To address this issue, herein, we rationally designed and optimized the structure of N, S co-doped VCT MXene, which exhibits excellent electrochemical performance with a high reversible capacity of 590 mAh g after 100 cycles at 0.1 A g when used as anode of LIBs. Even at a high current density of 2 A g, a reversible capacity of 298 mAh g is obtained after 300 cycles, which outperforms most of the VCT-based anode materials reported so far. The lithium-ion storage mechanism of N, S co-doped VCT MXene was studied by a series of characterizations. The results show that the significant improvement of electrochemical performance should be attributed to the facilitated charge transfer after N and S co-doping in VCT MXene, which can effectively improve the ion transfer kinetics during the lithiation-delithiation process. Furthermore, the expanded interlayer spacing of N, S co-doped VCT provides more active sites for the adsorption of lithium ions, promoting the insertion capacity of lithium ions. This work indicates that the N, S co-doped 2D VCT MXene should be a promising anode material for high-performance LIBs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2020.12.044DOI Listing
April 2021

Removal of Chlorine Ions from Desulfurization Wastewater by Modified Fly Ash Hydrotalcite.

ACS Omega 2020 Dec 4;5(49):31665-31672. Epub 2020 Dec 4.

Department of Environmental Science and Engineering, North China Electric Power University-Baoding Campus, Baoding 071003, P. R. China.

The effective removal of chlorine ion from the desulfurization slurry is of great significance to the stable operation of the desulfurization system. Modified fly ash hydrotalcites were prepared by alkali/acid-combined roasting and microwaving and used as an adsorbent for chlorine ion in desulfurized wastewater. The specific surface area and porosity of different adsorbents were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The impacts of pH, temperature, adsorbent dosage, and adsorption shaking time on adsorption performance were investigated. Results showed the alkali-combined roasting-modified fly ash hydrotalcite has the optimum removal effect on Cl. The optimal adsorption performance was achieved when the pH was 8, the adsorption temperature was 60 °C, the mass concentration of adsorbent was 10 g/L, the adsorption shaking time was 180 min, and the removal percentage of Cl was 68.1%. The adsorption isotherm was consistent with the Langmuir isotherm model, and the adsorption saturation was 694.4 mg/g, which belonged to monolayer adsorption.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c04074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745446PMC
December 2020

Modulation of Repeated Anodal HD-tDCS on Attention in Healthy Young Adults.

Front Psychol 2020 20;11:564447. Epub 2020 Nov 20.

Department of Military Medical Psychology, Air Force Military Medical University, Xi'an, China.

High-definition transcranial direct current stimulation (HD-tDCS) is a valid brain stimulation technology to optimize cognitive function. Recent evidence indicates that single anodal tDCS session enhances attention; however, the variation in attention produced by repeated anodal HD-tDCS over a longer period of time has not been explored. We examined the modulation of attention function in healthy young participants (39 young adults) who received repeated HD-tDCS sustained for 4 weeks. The results showed a robust benefit of anodal HD-tDCS on executive control and psychomotor efficiency, but not on orienting, alerting, or selective attention (inhibition); the benefit increased successively over 4 weeks; and the enhancement on executive control of each week was significant compared to baseline in the anodal group. In addition, the subjects' performances on the test of executive control and psychomotor efficiency gradually restored to the initial level in the sham group, which appeared obviously from week 3 (after 9 interventions), but the improvement of attention in the anodal group was persistent. We conclude that repeated anodal HD-tDCS provides a positive benefit on executive control and psychomotor efficiency and has obvious accumulative effect after 9 or more times intervention compared to sham HD-tDCS. Additionally, our findings might provide pivotal guidance for the formulation of a strategy for the use of repeated anodal HD-tDCS to modulate on attention function.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2020.564447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714753PMC
November 2020

Hydrated vanadium pentoxide/reduced graphene oxide composite cathode material for high-rate lithium ion batteries.

J Colloid Interface Sci 2021 Mar 25;585:347-354. Epub 2020 Nov 25.

Department of Electronic Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

As well-known, hydrated vanadium pentoxide (VO·nHO) has a larger layer spacing than orthogonal VO, which could offer more active sites to accommodate lithium ions, ensuring a high specific capacity. However, the exploration of VO·nHO cathode is limited by its inherently low conductivity and slow electrochemical kinetics, leading to a significant decrease in capability. Herein, we prepared VO·nHO/reduced graphene oxide (rGO) composite with low rGO content (8 wt%) via a simple yet effective dual electrostatic assembly strategy. When used as the cathode material for lithium-ion batteries (LIBs), VO·nHO/rGO manifests a high reversible capacity of 268 mAh g at 100 mA g and especially an excellent rate capability (196 mAh g at 1000 mA g and 129 mA h g at 2000 mA g), surpassing those of the VO/carbon composites reported in the literatures. Notably, the remarkable performance should be referable to the synergetic effects between one-dimensional VO·nHO nanobelts and two-dimensional rGO nanosheets, which provide a short transport pathway and enhanced electrical conductivity. This strategy opens a new opportunity for designing high-performance cathode material with excellent rate performance for advanced LIBs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2020.11.074DOI Listing
March 2021

Could the ambient higher temperature decrease the transmissibility of COVID-19 in China?

Environ Res 2021 02 3;193:110576. Epub 2020 Dec 3.

School of Public Health and Management, Ningxia Medical University, Yinchuan, 750001, Ningxia, China; The Key Laboratory of Environmental Factors and Chronic Disease Control of Ningxia, Yinchuan, 750001, Ningxia, China. Electronic address:

Background: Existing literatures demonstrated that meteorological factors could be of importance in affecting the spread patterns of the respiratory infectious diseases. However, how ambient temperature may influence the transmissibility of COVID-19 remains unclear.

Objectives: We explore the association between ambient temperature and transmissibility of COVID-19 in different regions across China.

Methods: The surveillance data on COVID-19 and meteorological factors were collected from 28 provincial level regions in China, and estimated the instantaneous reproductive number (R). The generalized additive model was used to assess the relationship between mean temperature and R.

Results: There were 12,745 COVID-19 cases collected in the study areas. We report the associated effect of temperature on R is likely to be negative but not of statistical significance, which holds for most Chinese regions.

Conclusions: We found little statistical evidence for that the higher temperature may reduce the transmissibility of COVID-19. Since intensive control measures against the COVID-19 epidemics were implemented in China, we acknowledge this may impact the underlying effect size estimation, and thus cautiousness should be taken when interpreting our findings.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envres.2020.110576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713592PMC
February 2021

Circ-ATP5H Induces Hepatitis B Virus Replication and Expression by Regulating miR-138-5p/ Axis.

Cancer Manag Res 2020 2;12:11031-11040. Epub 2020 Nov 2.

Department of Infectious Diseases, The People's Hospital of Danyang, Affiliated Danyang Hospital of Nantong University, Danyang City, Jiangsu Province, People's Republic of China.

Background: Circular RNAs (circRNAs) play an important regulatory role in various cancers, including hepatocellular carcinoma (HCC). This study aimed to investigate the function of hsa_circ_0006942 (circ-ATP5H) in hepatitis B virus (HBV)-associated HCC and its underlying mechanism.

Methods: The levels of circ-ATP5H, miR-138-5p and tumor necrosis factor alpha-induced protein 3 () were determined using quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot assay. The copies of HBV DNA were examined using qRT-PCR. The levels of hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) were detected via enzyme-linked immunosorbent assay (ELISA). Dual-luciferase reporter assay was used to analyze the interactions among circ-ATP5H, miR-138-5p and .

Results: Circ-ATP5H and levels were increased, while miR-138-5p level was reduced in HBV-positive HCC tissues and cells. Knockdown of circ-ATP5H hindered HBV DNA replication and decreased HBsAg and HBeAg levels in HBV-infected cells. Circ-ATP5H silencing suppressed HBV replication and expression by regulating miR-138-5p. Moreover, miR-138-5p blocked HBV replication and expression via targeting . Furthermore, circ-ATP5H up-regulated via absorbing miR-138-5p.

Conclusion: Circ-ATP5H promoted HBV replication and expression through modulating miR-138-5p/ axis, suggesting a new biomarker for HBV-related HCC treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/CMAR.S272983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648158PMC
November 2020

Socioeconomic disparity in the association between long-term exposure to PM and mortality in 2640 Chinese counties.

Environ Int 2021 Jan 5;146:106241. Epub 2020 Nov 5.

Department of Epidemiology and Preventive Medicine, School of Public Health and Preventive Medicine, Monash University, Melbourne, Australia. Electronic address:

Background: Although the association between long-term exposure to PM and mortality has been evaluated intensively, little is known about the socioeconomic disparity in the association.

Methods: We collected data on annual all-cause mortality, PM concentration, socioeconomic and demographic characteristics of 2640 counties from the two most recent Chinese censuses in 2000 and 2010. We applied the difference-in-differences (DID) method to estimate PM-mortality association for counties at different quartiles of literacy rate, college rate, urbanization rate and GDP per capita, respectively.

Results: Overall, every 10 µg/m increase in annual average PM was associated with 3.8% (95% confidence interval [CI]: 3.0-5.0) increase of all-cause mortality. The stratified analysis suggested higher health impact of exposure in counties with lower socioeconomic status. For counties of the lowest quartile (Q) of literacy rate, college rate, urbanization rate and GDP per capita, the effect estimates were 6.0% (95% CI: 4.2-7.7), 4.4% (95% CI: 2.8-6.0), 3.5% (95% CI: 2.0-5.1) and 4.9% (95% CI: 2.7-7.1), respectively. There was strong evidence for elevated risk in mortality associated with PM of all socioeconomic factors in the lowest quartile (Q) compared with the highest quartile counties (Q) (p-value for difference < 0.05).

Conclusions: There was socioeconomic disparity in the PM-mortality association in China. Dwellers living in less developed counties are more vulnerable to long-term exposure to ambient PM than those living in developed counties.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envint.2020.106241DOI Listing
January 2021

Effects of foliar selenium application on growth and rhizospheric soil micro-ecological environment of Koidz.

S Afr J Bot 2021 Mar 22;137:98-109. Epub 2020 Oct 22.

Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, Hubei, China.

(), a famous medicinal herb in China, is widely cultivated and consumed in China with various beneficial effects. Numerous studies have shown that selenium (Se) plays an important role in promoting plant growth, although Se has not been considered an essential element for higher plants. The objectives of this research were to determine the effects of foliar Se application (0, 2.5, 5.0, 10.0 and 20.0 mg m Se in sodium selenite, sprayed monthly from May to August) on the growth and rhizospheric soil micro-ecological environment of , and explore the possible mechanisms underlying plant response to foliar Se application through a field experiment. The results were: The foliar application of 5.0 mg m Se significantly increased the survival rate of compared to the control. The yield of was increased when the Se level maintained belowed 10.0 mg m but decreased when Se level reached 20.0 mg m. The Se content in the rhizome of showed a significant positive correlation with the Se level, while the insect attack rate was significantly negatively correlated with the Se level. However, foliar Se application hardly affected the concentration of bioactive compound atractylenolide in the rhizome of Notably, the application of foliar Se changed the content of partial soil nutrients, microbial diversity and composition in the rhizosphere soil of . Bacterial diversity was positively correlated with growth whereas fungal diversity was negatively correlated, suggesting that microbial diversity in the rhizosphere soils is closely related to plant growth. Moreover, correlation analysis showed that available potassium, and in rhizospheric soil might be critical factors for promoting the growth of . Overall, the foliar application of Se at moderate concentration was beneficial for the growth of and 5.0-10.0 mg m Se level was the optimum. Our findings revealed novel insights into the response of to foliar Se application from plant growth, rhizospheric soil nutrient and microbial community composition .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.sajb.2020.09.032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578779PMC
March 2021

circHIPK3 Acts as Competing Endogenous RNA and Promotes Non-Small-Cell Lung Cancer Progression through the miR-107/BDNF Signaling Pathway.

Biomed Res Int 2020 14;2020:6075902. Epub 2020 Sep 14.

Department of Respiratory Medicine, Minhang Hospital, Fudan University, China.

Circular RNAs (circRNAs) act as a crucial part in many human diseases, particularly in cancers. circRNA HIPK3 (circHIPK3) is a special circRNA that may participate in the oncogenesis of non-small-cell lung cancer (NSCLC), even though its latent regulatory mechanism is not very clear. Here, we studied the roles of circHIPK3 in NSCLC. qRT-PCR assay was applied to study the expression of circHIPK3 in NSCLC. The influence of circHIPK3 on NSCLC was estimated by silencing circHIPK3 and miR-107 mock transfection and brain-derived neurotrophic factor (BDNF) overexpression, and the correlation between circHIPK3, miR-107, and BDNF was evaluated by dual-luciferase reporter assay. The results showed that circHIPK3 expression was upregulated in NSCLC cells. circHIPK3 knockdown inhibited the migration and proliferation of NSCLC cells by promoting the expression of miR-107. circHIPK3 could be used as a miR-107 sponge to promote BDNF cell proliferation. The dual-luciferase reporter assay proved that miR-107 was the target of circHIPK3, and miR-107 had an interaction with the 3'untranslated region of BDNF. miR-107 overexpression inhibited BDNF-mediated NSCLC cell proliferation. These results indicate that circHIPK3 promotes tumor progression through a new circHIPK3/miR-107/BDNF axis, which offers potential markers and medical treatment for NSCLC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/6075902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7512038PMC
September 2020

Circular RNA circ-CPA4/ let-7 miRNA/PD-L1 axis regulates cell growth, stemness, drug resistance and immune evasion in non-small cell lung cancer (NSCLC).

J Exp Clin Cancer Res 2020 Aug 3;39(1):149. Epub 2020 Aug 3.

Department of Respiratory Medicine, Minhang Hospital, Fudan University, 170 Xin-Song Road, Shanghai, 201199, China.

Background: Non-small cell lung cancer (NSCLC) cells derived intracellular and extracellular programmed cell death ligand 1 (PD-L1) promoted cancer progression and drug resistance, and facilitated tumor immune evasion. However, the detailed molecular mechanisms are still largely unknown. In the present study, we aimed to explore the role of circular RNA circ-CPA4/let-7 miRNA/PD-L1 axis in the regulation of NSCLC progression, drug resistance and tumor immune microenvironment.

Methods: Real-Time qPCR and Western Blot analysis were conducted to examine gene expressions at transcriptional and translated levels, respectively. The regulatory mechanisms of circ-CPA4, let-7 miRNA and PD-L1 were validated by dual-luciferase reporter gene system and RNA pull-down assay. Cell growth and apoptosis were determined by CCK-8 assay, colony formation assay and Annexin V-FITC/PI double staining assay. Cell mobility was evaluated by transwell assay.

Results: Circ-CPA4 and PD-L1 were high-expressed, while let-7 miRNA was low-expressed in NSCLC cells and cancer tissues compared to the human bronchial epithelial (HBE) cells and their paired clinical normal adjacent tissues, respectively. Besides, knock-down of circ-CPA4 inhibited cell growth, mobility and epithelial-mesenchymal transition (EMT), and promoted cell death in NSCLC cells by downregulating PD-L1 through serving as a RNA sponge for let-7 miRNA. In addition, the NSCLC cells derived PD-L1-containing exosomes promoted cell stemness and increased resistance of NSCLC cells to cisplatin. Notably, by co-culturing the NSCLC cells with CD8 T cells isolated from human peripheral blood mononuclear cells (hPBMCs) in a transwell co-culturing system, we found that NSCLC cells inactivated CD8 T cells in a secreted PD-L1-dependent manner. Further results suggested that circ-CPA4 also positively regulated exosomal PD-L1, and the NSCLC cells with circ-CPA4 ablation re-activated CD8 T cells in the co-culturing system.

Conclusion: Taken together, circ-CPA4 regulated cell growth, mobility, stemness and drug resistance in NSCLC cells and inactivated CD8 T cells in the tumor immune microenvironment through let-7 miRNA/PD-L1 axis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13046-020-01648-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397626PMC
August 2020

Regeneration of Selective Catalyst Reduction Catalysts Deactivated by Pb, As, and Alkali Metals.

ACS Omega 2020 Jun 7;5(23):13886-13893. Epub 2020 Jun 7.

Hebei Key Lab of Power Plant Flue Gas Multi-Pollutants Control, Department of Environmental Science and Engineering, North China Electric Power University-Baoding Campus, Baoding 071003, P. R. China.

Owing to increased operating time and Pb, As, and alkali metal poisoning of a catalyst, the activity of the catalyst is lowered. In the present study, we utilized the acetic acid and the traditional sulfuric acid pickling process for regeneration and then performed Brunauer-Emmett-Teller (BET) specific surface area, denitrification efficiency, scanning electron microscopy, and X-ray fluorescence (XRF) analysis of a fresh catalyst, a deactivated catalyst, and a regenerated catalyst for comparison purposes. The experimental results demonstrated that the removal ratios of Pb, As, Na, and K were 99.2, 98.8, 99.9, and 93.9%, respectively. Compared to the traditional sulfuric acid regeneration technology, the acetic acid regeneration technology eliminates the activated liquid immersion step; therefore, the steps are simpler and efficient for the regeneration of selective catalyst reduction catalysts deactivated by Pb, As, and alkali. The current study provides a new method for the regeneration and application of selective catalyst reduction (SCR) catalysts, which is particularly applicable for regenerating a large number of Pb, As, and alkali-metal poisoned catalysts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c01283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301543PMC
June 2020

Simultaneous Desulfurization and Denitrification Using La-Ce-V-Cu-ZSM-5 Catalysts in an Electrostatic Precipitator.

ACS Omega 2020 May 28;5(18):10525-10532. Epub 2020 Apr 28.

Hebei Key Laboratory of Power Plant Flue Gas Multi-Pollutant Control, Department of Environmental Science and Engineering, North China Electric Power University-Baoding Campus, Baoding 071003, P. R. China.

Different catalysts were loaded onto the collecting plate of an electrostatic precipitator to achieve the simultaneous removal of multiple pollutants from coal-fired gas. The synergistic desulfurization and denitrification effect of the catalyst and the effect of corona discharge on the activity of the catalyst were studied. The La(6%)-Ce(8%)-V(7%)-Cu(8%)-ZSM-5 catalyst prepared by successive impregnation methods had the optimum simultaneous desulfurization and denitrification efficiency at a roasting temperature of 600 °C. The desulfurization and denitrification rates reached 97.09 and 83.30%, respectively. BET and SEM characterization results showed that the loading of active components and additives improved the pore structure of the molecular sieve, which contributed to the high stability of the catalyst's internal structure and large surface area, as well as better desulfurization and denitrification efficiency. Corona discharge can significantly improve the catalytic effect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c00808DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227058PMC
May 2020

Microenvironmental Th9 and Th17 lymphocytes induce metastatic spreading in lung cancer.

J Clin Invest 2020 07;130(7):3560-3575

Max Planck Institute for Heart and Lung Research, Department of Lung Development and Remodeling, member of the German Center for Lung Research (DZL), member of Cardio-Pulmonary Institute (CPI), Bad Nauheim, Germany.

Immune microenvironment plays a critical role in lung cancer control versus progression and metastasis. In this investigation, we explored the effect of tumor-infiltrating lymphocyte subpopulations on lung cancer biology by studying in vitro cocultures, in vivo mouse models, and human lung cancer tissue. Lymphocyte conditioned media (CM) induced epithelial-mesenchymal transition (EMT) and migration in both primary human lung cancer cells and cell lines. Correspondingly, major accumulation of Th9 and Th17 cells was detected in human lung cancer tissue and correlated with poor survival. Coculturing lung cancer cells with Th9/Th17 cells or exposing them to the respective CM induced EMT in cancer cells and modulated the expression profile of genes implicated in EMT and metastasis. These features were reproduced by the signatory cytokines IL-9 and IL-17, with gene regulatory profiles evoked by these cytokines partly overlapping and partly complementary. Coinjection of Th9/Th17 cells with tumor cells in WT, Rag1-/-, Il9r-/-, and Il17ra-/- mice altered tumor growth and metastasis. Accordingly, inhibition of IL-9 or IL-17 cytokines by neutralizing antibodies decreased EMT and slowed lung cancer progression and metastasis. In conclusion, Th9 and Th17 lymphocytes induce lung cancer cell EMT, thereby promoting migration and metastatic spreading and offering potentially novel therapeutic strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1172/JCI124037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324180PMC
July 2020

SO absorption in pure ionic liquids: Solubility and functionalization.

J Hazard Mater 2020 06 9;392:122504. Epub 2020 Mar 9.

School of Safety Science and Engineering, Henan Polytechnic University, Jiaozuo, 454003, China; Collaborative Innovation Center of Coal Safety Production of Henan Province, Jiaozuo, 454003, China; State Key Laboratory Cultivation Base for Gas Geology and Gas Control in Henan Polytechnic University, Jiaozuo, 454003, China. Electronic address:

The SO solubility in ionic liquids and absorption mechanisms with different functionalities, including ether, halide, carboxylate, dicarboxylate, thiocynate, phenol, amino, azole groups, etc., are presented in this review. Strategies of improving SO capture with low binding energy and the separation performance from CO are also concluded. Generally, moderate basicity is favourable for enhancing SO capacity and the water (below 6 wt%) effect on absorption is indefinite but generally slight. Introducing electron-withdrawing substituents such as nitrile, halogen, aldehyde and carboxylic groups are proposed to decrease the chemical absorption enthalpy between ionic liquid and SO in order to reduce regeneration power consumption. Although it is promising, the absorption enthalpy is still much higher than the physisorption performance especially of the ether-functionalized ones. The biocompatible choline-based, betaine-based, and amino acid ionic liquids have clear trends to be applied in SO capture due to their biodegradability, nontoxicity and easy accessibility. Generally, comparing to the traditional solvents, ionic liquids have made great improvement in SO capacity, however, the high viscosity and desorption energy are two main obstacles for SO absorption and separation. Molecular simulations have been applied to reveal the absorption regimes involving the roles of basic functionalities and physical interactions especially the hydrogen bonds, which could be referred for structure designing of the available ionic liquids with readily fluid characteristics and absorption ability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2020.122504DOI Listing
June 2020

Iridium-Catalyzed γ-Selective Hydroboration of γ-Substituted Allylic Amides.

Org Lett 2020 Apr 23;22(7):2861-2866. Epub 2020 Mar 23.

State Key Laboratory for Oxo Synthesis and Selective Oxidation, Center for Excellence in Molecular Science, Suzhou Research Institute, Lanzhou Institute of Chemical Physics, Chinese Academy of Science, Lanzhou 730000, China.

Reported here for the first time is the Ir-catalyzed γ-selective hydroboration of γ-substituted allylic amides under mild reaction conditions. A variety of functional groups could be compatible with reaction conditions, affording γ-branched amides in good yields with ≤97% γ-selectivity. We have also demonstrated that the obtained borylated products could be used in a series of C-O, C-F, C-Br, and C-C bond-forming reactions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.orglett.0c00977DOI Listing
April 2020

The association between ambient temperature and clinical visits for inflammation-related diseases in rural areas in China.

Environ Pollut 2020 Jun 8;261:114128. Epub 2020 Feb 8.

Department of Epidemiology and Preventive Medicine, School of Public Health and Preventive Medicine, Monash University, Melbourne, VIC, Australia.

Background: The association between temperature and mortality has been widely reported. However, it remains largely unclear whether inflammation-related diseases, caused by excessive or inappropriate inflammatory reaction, may be affected by ambient temperature, particularly in low-income areas.

Objectives: To explore the association between ambient temperature and clinical visits for inflammation-related diseases in rural villages in the Ningxia Hui Autonomous Region, China, during 2012─2015.

Methods: Daily data on inflammation-related diseases and weather conditions were collected from 258 villages in Haiyuan (161 villages) and Yanchi (97 villages) counties during 2012─2015. A Quasi-Poisson regression with distributed lag non-linear model was used to examine the association between temperature and clinical visits for inflammation-related diseases. Stratified analyses were performed by types of diseases including arthritis, gastroenteritis, and gynecological inflammations.

Results: During the study period, there were 724,788 and 288,965 clinical visits for inflammation-related diseases in Haiyuan and Yanchi, respectively. Both exposure to low (RR: 2.045, 95% CI: 1.690, 2.474) and high temperatures (RR: 1.244, 95% CI: 1.107, 1.399) were associated with increased risk of total inflammation-related visits in Haiyuan county. Low temperatures were associated with increased risks of all types of inflammation-related diseases in Yanchi county (RR: 4.344, 95% CI: 2.887, 6.535), while high temperatures only affected gastroenteritis (RR: 1.274, 95% CI: 1.040, 1.561). Moderate temperatures explained approximately 26% and 33% of clinical visits due to inflammation-related diseases in Haiyuan and Yanchi, respectively, with the burden attributable to cold exposure higher than hot exposure. The reference temperature values ranged from 17 to 19 in Haiyuan, and 12 to 14 in Yanchi for all types of clinical visits.

Conclusions: Our findings add additional evidence for the adverse effect of suboptimal ambient temperature and provide useful information for public health programs targeting people living in rural villages.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2020.114128DOI Listing
June 2020

Characterization of a novel aldo-keto reductase with anti-Prelog stereospecificity from Corallococcus sp. EGB.

Int J Biol Macromol 2020 Mar 27;146:36-44. Epub 2019 Dec 27.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China. Electronic address:

The asymmetric reduction of prochiral ketones is a promising process for synthesis of optically active alcohols. The aldo-keto reductase (AKR) is an attractive candidate of biocatalyst, due to its high enantioselectivity and environmentally friendly reaction conditions. In this work, nine putative AKR encoding genes from Corallococcus sp. EGB were cloned and expressed in Escherichia coli. Of these produced enzymes (CoAKRs), CoAKR7 exhibited reductive activity to various ketones and ketoesters, especially very high activity toward ethyl 4-chloro-3-oxobutanoate (COBE) with NADPH as the coenzyme. The CoAKR7 was optimally active at pH 7.0 and 50 °C. The apparent K and V for COBE was 14.18 U/mg and 0.269 mM, respectively. Moreover, CoAKR7 catalyzed an anti-Prelog reduction of COBE to (S)-ethyl-4-chloro-3-hydroxybutanoate (CHBE) with e.e. >99%. Enzyme-substrate-cofactor docking analysis elucidated the molecular mechanism of the substrate stereospecificity, providing basis for protein engineering of these enzymes for applications in the synthesis of valuable chemicals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2019.12.214DOI Listing
March 2020

IL-17 CD8 T cell suppression by dimethyl fumarate associates with clinical response in multiple sclerosis.

Nat Commun 2019 12 16;10(1):5722. Epub 2019 Dec 16.

Institute for Medical Microbiology and Hospital Hygiene, University of Marburg, 35043, Marburg, Germany.

IL-17-producing CD8 (Tc17) cells are enriched in active lesions of patients with multiple sclerosis (MS), suggesting a role in the pathogenesis of autoimmunity. Here we show that amelioration of MS by dimethyl fumarate (DMF), a mechanistically elusive drug, associates with suppression of Tc17 cells. DMF treatment results in reduced frequency of Tc17, contrary to Th17 cells, and in a decreased ratio of the regulators RORC-to-TBX21, along with a shift towards cytotoxic T lymphocyte gene expression signature in CD8 T cells from MS patients. Mechanistically, DMF potentiates the PI3K-AKT-FOXO1-T-BET pathway, thereby limiting IL-17 and RORγt expression as well as STAT5-signaling in a glutathione-dependent manner. This results in chromatin remodeling at the Il17 locus. Consequently, T-BET-deficiency in mice or inhibition of PI3K-AKT, STAT5 or reactive oxygen species prevents DMF-mediated Tc17 suppression. Overall, our data disclose a DMF-AKT-T-BET driven immune modulation and suggest putative therapy targets in MS and beyond.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-019-13731-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915776PMC
December 2019

Impact of maltogenic α-amylase on the structure of potato starch and its retrogradation properties.

Int J Biol Macromol 2020 Feb 13;145:325-331. Epub 2019 Dec 13.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.

Structural modification of starch using efficient α-amylases to improve its properties is an established method in the starch industry. In our previous research, the novel maltogenic α-amylase CoMA that catalyzes multi-molecular reactions has been identified. In this study, the impact of CoMA on the structure and retrogradation properties of potato starch was evaluated. CoMA cleaves internal starch chains to change the proportion of amylose and amylopectin in starch. Following treatment, visible pores and microporous on the surface of starch granules were observed from SEM analysis. CoMA modification led to increased insoluble blue complex formation and hydrolysis to shorten the outer chains, which was found to reduce the development rate of starch according to network interactions from the dynamic rheological analysis. Furthermore, maltose accumulation with water competition was also deduced to be involved in the inhibition of retrogradation. Its activities in the cleavage of internal starch granules, shortening of outer chains of starch, and maltose formation make CoMA a powerful agent for the inhibition of starch retrogradation with a very low effective dose of 0.5 mg/kg, which may find potential applications in the starch processing industry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2019.12.098DOI Listing
February 2020

Outcomes of the Warden Procedure for Partial Anomalous Pulmonary Venous Drainage.

Pediatr Cardiol 2020 Jan 6;41(1):134-140. Epub 2019 Dec 6.

Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 167, North Lishi Street, Xicheng District, Beijing, 100037, China.

Partial anomalous pulmonary venous drainage (PAPVD) is a common congenital heart disease. If the insertion of an anomalous pulmonary vein (PV) is high into the superior vena cava (SVC), the traditional 1-patch or 2-patch surgical repair might be challenging. Unlike patch procedures, the cavoatrial anastomosis technique (Warden procedure) theoretically reduces complications such as sinus node dysfunction and venous obstruction. We investigate outcomes of the Warden procedure (WP) at a single institution. A total of 67 patients (42 males and 25 females) with PAPVD who underwent the WP between January 2011 and December 2018 were consecutively enrolled. The median age was 2.8 years (3-61 years old). 52 cases were younger than 18 years old. perioperative and follow-up clinical data were collected. In addition, we selected possible risk factors (a total of 18 risk factors) of SVC stenosis or obstruction. Univariate and multivariate analyses were conducted to confirm the independent risk factors. The average Cardiopulmonary bypass (CPB) time was 132.3 ± 46.3 min, and the aortic cross-clamp time was 85.2 ± 35.7 min. One patient died postoperatively in hospital. The mean follow-up time of the remaining patients (n = 66) was 15.8 ± 14.5 months (3-64 months). No abnormal cardiac function or sinus node dysfunction cases were identified during the follow-up. Of the survival patients, no venous obstruction was presented before the discharge. Numbers of patients identified with mild PV and SVC stenosis were 1 (1.5%) and 7 (10.6%, 4 of them turned negative eventually) at discharge. At the last follow-up, no PV obstruction was identified, whereas, 4 cases (6%) had SVC obstruction. Numbers of PV and SVC stenosis cases were 3 (4.5%) and 8 (12%). Univariate and multivariate analyses showed that only the diameter of SVC less than 10 mm was an independent risk factor for SVC stenosis or obstruction at the last follow-up. Warden procedure can effectively treat PAPVD (anomalous drainages into SVC), with satisfactory early and mid-term postoperative results. Different modifications of the Warden procedure may be selected according to the anatomic characteristics. The diameter of SVC less than 10 mm predicts SVC stenosis or obstruction after Warden procedure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00246-019-02235-8DOI Listing
January 2020

Construction and analysis of a diabetic nephropathy related protein-protein interaction network reveals nine critical and functionally associated genes.

Comput Biol Chem 2019 Dec 30;83:107115. Epub 2019 Aug 30.

Department of Bioinformatics, Chongqing Medical University, Chongqing 400016, China. Electronic address:

Diabetic nephropathy (DN) is one of the common diabetic complications, but the mechanisms are still largely unknown. In this study, we constructed a DN related protein-protein interaction network (DNPPIN) on the basis of RNA-seq analysis of renal cortices of DN and normal mice, and the STRING database. We analyzed DNPPIN in detail revealing nine critical proteins which are central in DNPPIN, and contained in one network module which is functionally enriched in ribosome, nucleic acid binding and metabolic process. Overall, this study identified nine critical and functionally associated protein-coding genes concerning DN. These genes could be a starting point of future research towards the goal of elucidating the mechanisms of DN pathogenesis and progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.compbiolchem.2019.107115DOI Listing
December 2019

Recommended storage temperature for green tea based on sensory quality.

J Food Sci Technol 2019 Sep 1;56(9):4333-4348. Epub 2019 Jul 1.

1State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, 230036 Anhui China.

This study aimed to evaluate the effect of storage temperature on the alteration of the sensory quality of tea. Huangshan Maofeng tea was stored at - 80 °C, - 20 °C, 4 °C, or room temperature for up to 150 days. The physicochemical parameters, taste-related components, appearance color, volatile compounds and sensory quality of tea were analyzed and compared. Results showed that storing tea at - 80 °C and - 20 °C effectively preserved the physicochemical parameters, taste-related compounds and appearance color in tea. Multivariate statistical analysis (PCA and OPLS-DA) indicated that tea stored at - 80 °C exhibited a similar volatiles composition as fresh tea based on gas chromatography-mass spectrometry, whereas the composition of volatiles was significantly altered in tea stored at 4 °C after 100 days of storage. Sensory evaluation illustrated that tea stored at - 80 °C and - 20 °C remained the freshness regarding leaves appearance and tea infusion color, taste and aroma, whereas an obvious decrease on the tea freshness was found in tea stored at 4 °C and room temperature. These findings indicated that storage temperature played a vital role in altering the aromatic and sensory quality of Huangshan Maofeng tea and the recommended tea storage temperature was - 80 °C or - 20 °C.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13197-019-03902-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706524PMC
September 2019

α-Ketoglutarate-Activated NF-κB Signaling Promotes Compensatory Glucose Uptake and Brain Tumor Development.

Mol Cell 2019 10 22;76(1):148-162.e7. Epub 2019 Aug 22.

State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Innovation Center for Cell Signaling Network, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China; Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China. Electronic address:

The rapid proliferation of cancer cells and dysregulated vasculature within the tumor leads to limited nutrient accessibility. Cancer cells often rewire their metabolic pathways for adaption to nutrient stress, and the underlying mechanism remains largely unknown. Glutamate dehydrogenase 1 (GDH1) is a key enzyme in glutaminolysis that converts glutamate to α-ketoglutarate (α-KG). Here, we show that, under low glucose, GDH1 is phosphorylated at serine (S) 384 and interacts with RelA and IKKβ. GDH1-produced α-KG directly binds to and activates IKKβ and nuclear factor κB (NF-κB) signaling, which promotes glucose uptake and tumor cell survival by upregulating GLUT1, thereby accelerating gliomagenesis. In addition, GDH1 S384 phosphorylation correlates with the malignancy and prognosis of human glioblastoma. Our finding reveals a unique role of α-KG to directly regulate signal pathway, uncovers a distinct mechanism of metabolite-mediated NF-κB activation, and also establishes the critical role of α-KG-activated NF-κB in brain tumor development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.molcel.2019.07.007DOI Listing
October 2019

IFN-DC Loaded with Autophagosomes containing Virus Antigen is Highly Efficient in Inducing Virus-Specific Human T Cells.

Int J Med Sci 2019 10;16(5):741-750. Epub 2019 May 10.

Clinical Research Center, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine. Zhong Fu Road, Gulou District, Nanjing, Jiangsu, PR China 210003.

Autophagy plays a critical role in the regulation of innate and adaptive immune responses to pathogens and tumors. A previous study utilized proteasome and lysosome inhibitors to form autophagosomes (DRibbles) and the effect of dendritic cells (DCs) loaded with DRibbles in activating antigen-specific T cells has been demonstrated in a mouse experiment and human IL-4-DC. In this study, CMV-DRibbles derived from MDA cell lines expressing cytomegalovirus (CMV) pp65 protein were loaded onto human IFN-DC and IL-4-DC derived from monocytes, respectively. We observed that CMV-DRibbles resulted in the up-regulation of HLA-DR, CD11c, and CD83, but not co-stimulatory molecules CD 80 and CD86 on IFN-DC. Meanwhile, the expression of HLA-DR, CD80, CD83, and CD86, except for CD11c on IL-4-DC loaded with CMV-DRibbles were up-regulated. Moreover, CMV-DRibbles had no ability to stimulate these two moDCs to secrete cytokines IL-6, IL-1β and IL-10. Then, we optimized the conditions for antigen up-take by DCs and found that mature moDCs had a superior ability to up-take CMV-DRibbles compared with immature DCs in a dose-dependent manner. Furthermore, the efficiency of CMV-DRibbles up-take by IFN-DC was superior compared to IL-4-DC. Finally, we observed that mIFN-DC was significantly more efficient at stimulating autologous CMV-specific CD4 T cells (0.39 vs. 0.28 %, <0.05) and CD8 T cells (0.36 vs. 0.12%, <0.05) to secrete IFN-γ compared with mIL-4-DC. Therefore, DRibbles containing specific viral antigens were efficient activators of human antigen-specific T cells. Our results demonstrated that IFN-DC loaded with CMV-DRibbles revealed a superior ability to induce CMV-specific T cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/ijms.31830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566749PMC
December 2019

Effect of early highly active antiretroviral therapy on viral suppression among newly diagnosed men who have sex with men living with human immunodeficiency virus in Xi'an, China.

J Med Virol 2019 07 27;91(7):1263-1271. Epub 2019 Mar 27.

Department of Sexually Transmitted Diseases Prevention and Control, Xi'an Centers for Disease Control and Prevention, Xi'an, China.

Background: The number of men who have sex with men (MSM) living with human immunodeficiency virus (HIV) in China has increased rapidly and thus immediate highly active antiretroviral therapy (HAART) after diagnosis was implemented as a strategy to reduce the HIV transmission.

Methods: MSM who were diagnosed with HIV and received HAART between 2013 to 2015 in Xi'an were divided into three groups (>350, 200-350, and <200 cell/μL) according to their baseline CD4+ T cell count. The time of follow-up was calculated from the first date of receiving HAART to December 31, 2016. The CD4+ T cell count was detected with 1 week before or after HAART. The plasma viral loads were tested after 1, 2, and 3 years of treatment.

Results: Of 1442 subjects who received HAART, 690 (47.9%) cases were in >350 cell/μL group, whereas 400 (27.7%) cases and 352 (24.4%) cases were in the 200-350 cell/μL group and <200 cell/μL group, respectively. After 1 year of treatment, the viral suppression rate in the <200 cell/μL group was 91.1%, which was significantly lower than the other two groups. The logistic regression results show that the >350 cell/μL group and 200-350 cell/μL group predicted higher viral suppression rates.

Conclusions: Baseline CD4+ T cell count more than 350 cell/μL can improve viral suppression among MSM living with HIV. Furthermore, to reduce the transmission risk, the treatment compliance of people living with HIV with high CD4+ T cell levels should be improved, and their diagnosis to the treatment time should be decreased.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jmv.25449DOI Listing
July 2019

Tyrosine phosphorylation activates 6-phosphogluconate dehydrogenase and promotes tumor growth and radiation resistance.

Nat Commun 2019 03 1;10(1):991. Epub 2019 Mar 1.

State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Innovation Center for Cell Signaling Network, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 200031, Shanghai, China.

6-Phosphogluconate dehydrogenase (6PGD) is a key enzyme that converts 6-phosphogluconate into ribulose-5-phosphate with NADP as cofactor in the pentose phosphate pathway (PPP). 6PGD is commonly upregulated and plays important roles in many human cancers, while the mechanism underlying such roles of 6PGD remains elusive. Here we show that upon EGFR activation, 6PGD is phosphorylated at tyrosine (Y) 481 by Src family kinase Fyn. This phosphorylation enhances 6PGD activity by increasing its binding affinity to NADP and therefore activates the PPP for NADPH and ribose-5-phosphate, which consequently detoxifies intracellular reactive oxygen species (ROS) and accelerates DNA synthesis. Abrogating 6PGD Y481 phosphorylation (pY481) dramatically attenuates EGF-promoted glioma cell proliferation, tumor growth and resistance to ionizing radiation. In addition, 6PGD pY481 is associated with Fyn expression, the malignancy and prognosis of human glioblastoma. These findings establish a critical role of Fyn-dependent 6PGD phosphorylation in EGF-promoted tumor growth and radiation resistance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-019-08921-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397164PMC
March 2019

Heavy metal poisoned and regeneration of selective catalytic reduction catalysts.

J Hazard Mater 2019 03 7;366:492-500. Epub 2018 Dec 7.

School of Environmental Science and Engineering, North China Electric Power University, Baoding, 071003, China.

Being poison to heterogeneous catalysts, Pb and As are important metals in the study of industrial NO emission reductions. A series of VO-WO/TiO-SiO catalysts poisoned by Pb and As were prepared through wet impregnation. The regeneration effect of deionized water, acid solution, complexing agent, and alkali solution on a poisoned catalyst was evaluated through experimental and theoretical calculations. For a Pb-poisoned catalyst, deionized water, acetic acid, and triethylenetetramine were used to wash. NO conversion rate on the Pb-VO-WO/TiO-SiO catalyst increased from approximately 59.49% to 95.70%, which is 98.43% of the efficiency of the original fresh catalyst. As for an As-poisoned catalyst, deionized water, acetic acid solution, and sodium carbonate solution were used to wash, and the NO conversion rate on the As-VO-WO/TiO-SiO catalyst increased from approximately 64.05% to 80.76%, which is 98.45% of the original efficiency of the fresh catalyst. The specific surface areas of the lead and arsenic regeneration catalyst were 46.41 m/g and 45.95 m/g respectively, approximately 14.00% and 11.02% higher than that of the poisoned catalyst (40.71 m/g and 41.39 m/g).The study provides a new method for the regeneration of SCR catalysts, which is particularly applicable for regenerating a large number of Pb- and As-poisoned catalysts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2018.11.112DOI Listing
March 2019