Publications by authors named "Yajuan Li"

199 Publications

Isoginkgetin treatment attenuated lipopolysaccharide-induced monoamine neurotransmitter deficiency and depression-like behaviors through downregulating p38/NF-κB signaling pathway and suppressing microglia-induced apoptosis.

J Psychopharmacol 2021 Jul 19:2698811211032473. Epub 2021 Jul 19.

Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong, China.

Background: Microglia activation-induced neuroinflammation may contribute to the etiology of depression. containing high concentration of isoginkgetin could effectively treat mental diseases in ancient times. However, the therapeutic role, peculiarly in the brain-immune modulation in depression is still unclear. This study aimed to determine effects of isoginkgetin on lipopolysaccharide (LPS)-induced depression-like changes. Furthermore, its modulation on the p38/nuclear factor-kappa B (NF-κB) pathway in LPS-activated microglia was evaluated.

Methods: Adult Kunming mice were intraperitoneally injected vehicle or isoginkgetin (4 mg/kg) daily for 14 days before saline or LPS (0.83 mg/kg) administration. Depression-like behavior, neurotransmitter levels, and markers of neuroinflammation were determined. Isoginkgetin effect on LPS-induced microglial activation was then assessed in BV2 cells. Finally, conditioned medium (CM) derived from isoginkgetin-treated BV2 cells was co-cultured with SH-SY5Y cells for 24 h. Cell viability and apoptosis were evaluated.

Results: LPS significantly induced helplessness and anxiety, which were associated with decreased 5-HT, noradrenaline, and dopamine concentrations. Meanwhile, LPS increased microglia M1 hallmark Iba1 expression and serum interleukin (IL)-1β concentration. These changes were attenuated by isoginkgetin treatment. In vitro, isoginkgetin markedly suppressed the production of IL-1β, IL-6, tumor necrosis factor-alpha, cyclooxygenase-2, inducible nitric oxide, and reactive oxygen species, which are released from LPS-stimulated BV2 cells. More interestingly, CM from isoginkgetin-treated BV2 cells significantly alleviated SH-SY5Y cell apoptosis and restored cell viability compared to LPS-treated group through the inhibition of p38/NF-κB signaling pathway.

Conclusion: These data demonstrate that isoginkgetin is an effective therapeutic agent for depression-like behaviors and neuropathological changes via potent anti-inflammatory property.
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http://dx.doi.org/10.1177/02698811211032473DOI Listing
July 2021

Unveiling Crystal Orientation in Quasi-2D Perovskite Films by In Situ GIWAXS for High-Performance Photovoltaics.

Small 2021 Jul 12:e2100972. Epub 2021 Jul 12.

Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials (FUNSOM), Soochow University, Suzhou, 215123, P. R. China.

Quasi-2D perovskites are enchanting alternative materials for solar cells due to their intrinsic stability. The manipulation of crystal orientation of quasi-2D perovskites is indispensable to target efficient devices, however, the origin of orientation during the film fabrication process still lacks in-depth understanding and convincing evidence yet, which hinders further boosting the performance of photovoltaic devices. Herein, the crystallizing processes during spin-coating and annealing are probed by in situ grazing-incidence wide-angle X-ray scattering (GIWAXS), and the incident-angle-dependent GIWAXS is conducted to unveil the phase distribution in the films. It is found that undesirable lead iodide sol-gel formed intermediate phase would disturb oriented crystalline growth, resulting in random crystal orientation in poor quasi-2D films. A general strategy is developed via simple additive agent incorporation to suppress the formation of the intermediate phase. Accordingly, highly oriented perovskite films with reduced trap density and higher carrier mobility are obtained, which enables the demonstration of optimized quasi-2D perovskite solar cells with a power conversion efficiency of 15.2% as well as improved stability. This work paves a promising way to manipulate the quasi-2D perovskites nucleation and crystallization processes via tuning nucleation stage.
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http://dx.doi.org/10.1002/smll.202100972DOI Listing
July 2021

Inherited unbalanced translocation (4p16.3p15.32 duplication/8p23.3p23.2deletion) in the four generation pedigree with intellectual disability/developmental delay.

Mol Cytogenet 2021 Jul 8;14(1):35. Epub 2021 Jul 8.

Department of Reproductive Medicine Center, General Hospital of Northern Theater Command, Shenyang, Liaoning, 110016, People's Republic of China.

Chromosomal copy number variants (CNVs) are an important cause of congenital malformations and mental retardation. This study reported a large Chinese pedigree (4-generation, 76 members) with mental retardation caused by chromosome microduplication/microdeletion. There were 10 affected individuals with intellectual disability (ID), developmental delay (DD), and language delay phenotypes. SNP array analysis was performed in the proband and eight patients and found all of them had a microduplication of chromosome 4p16.3p15.2 and a microdeletion of chromosome 8p23.3p23.2. The high-resolution karyotyping analysis of the proband had unbalanced karyotype [46, XY, der(8)t(4;8)(p15.2;p23.1)mat], his mother had balanced karyotype [46, XX, t(4;8) (p15.2;p23.1)], whereas his father had normal karyotype [46,XY]. Fluorescence in situ hybridization (FISH) analysis further confirmed that the proband's mother had a balanced translocation between the short arm terminal segment of chromosome 4 and the short arm end segment of chromosome 8, ish t(4;8)(8p + ,4q + ;4p + ,8q +). In conclusion, all the patients inherited chromosomes 8 with 4p16.3p15.2 duplication and 8p23.3p23.2 deletion from their parental balanced translocation, which might be the cause of the prevalence of intellectual disability. Meanwhile, 8p23.3p23.2 deletion, rather than 4p16.3p15.2 duplication might cause a more severe clinical syndrome.
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http://dx.doi.org/10.1186/s13039-021-00552-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268195PMC
July 2021

MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice.

Int J Mol Sci 2021 Jun 21;22(12). Epub 2021 Jun 21.

Center of Experimental Teaching for Common Basic Courses, South China Agricultural University, Guangzhou 510642, China.

The developmental process of inflorescence and gametophytes is vital for sexual reproduction in rice. Multiple genes and conserved miRNAs have been characterized to regulate the process. The changes of miRNAs expression during the early development of rice inflorescence remain unknown. In this study, the analysis of miRNAs profiles in the early stage of rice inflorescence development identified 671 miRNAs, including 67 known and 44 novel differentially expressed miRNAs (DEMs). Six distinct clusters of miRNAs expression patterns were detected, and Cluster 5 comprised 110 DEMs, including unconserved, rice-specific osa-miR5506. Overexpression of osa-miR5506 caused pleiotropic abnormalities, including over- or under-developed palea, various numbers of floral organs and spikelet indeterminacy. In addition, the defects of ovaries development were frequently characterized by multiple megasporocytes, ovule-free ovary, megasporocyte degenerated and embryo sac degenerated in the transgenic lines. osa-miR5506 targeted REM transcription factor . Summarily, these results demonstrated that rice-specific osa-miR5506 plays an essential role in the regulation of floral organ number, spikelet determinacy and female gametophyte development in rice.
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http://dx.doi.org/10.3390/ijms22126610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235126PMC
June 2021

LPS Mediates Bovine Endometrial Epithelial Cell Pyroptosis Directly Through Both NLRP3 Classical and Non-Classical Inflammasome Pathways.

Front Immunol 2021 28;12:676088. Epub 2021 May 28.

Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agricultural and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Science, Chinese Academy of Agricultural Science, Lanzhou, China.

As a highly inflammatory form of programmed cell death, pyroptosis is triggered by pro-inflammatory signals and associated with inflammation. It is characterized by cell swelling and large bubbles emerging from the plasma membrane, which release cytokines during inflammation. Compared with other types of cell death, pyroptosis has a distinct morphology and mechanism and involves special inflammasome cascade pathways. However, the inflammasome mechanism through which endometrial epithelial cell pyroptosis occurs in LPS-mediated inflammation remains unclear. We confirmed that there was an increased mRNA and protein expression of the IL-6, TNF-α, IL-1β, IL-18 cytokines, the inflammasome molecules NLRP3, CASPASE-1, CASPASE-4, and GSDMD in LPS-induced primary bovine endometrial epithelial cells (BEECs) in an established inflammatory model using ELISA, real-time PCR (RT-PCR), vector construction and transfection, and Western blotting. Scanning electron microscopy and lactate dehydrogenase (LDH) activity assays revealed induced cell membrane rupture, which is the main characteristic of pyroptosis. In conclusion, the cytolytic substrate GSDMD's cleavage by caspase-1 or caspase-4 through the NLRP3 classical and non-classical inflammasome pathways, GSDMD N-terminus bind to the plasma membrane to form pores and release IL -18, IL-1β cause cell death during LPS induced BEECs inflammation.
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http://dx.doi.org/10.3389/fimmu.2021.676088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195237PMC
May 2021

Antimicrobial effect of tea polyphenols against foodborne pathogens.

J Food Prot 2021 Jun 4. Epub 2021 Jun 4.

Department of Food Science and Engineering, Ningbo University, Ningbo 315211, P.R. China.

In recent years, science and technology have developed to a considerable level. However, food contamination by food-borne pathogens is still widespread in many countries around the world, and food safety is a major global public health issue. Therefore, novel preservatives that can guarantee safer food are high in demand. Contrary to artificial food preservatives, tea polyphenols (TP) are getting wide attention as food additives for being "green", "safe" and "healthy". The sources of TP are wide, and the purification technology is sophisticated. Compared with other natural antibacterial agents, its antibacterial effect is more stable. It is an excellent natural antibacterial agent. Here, this review systematically summarizes the important chemical components of TP and discusses their antibacterial mechanisms against various foodborne pathogens. In addition, the potential application areas of TP are also discussed. It can provide a theoretical basis for the in-depth study of TP.
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http://dx.doi.org/10.4315/JFP-21-043DOI Listing
June 2021

Modulation of Bovine Endometrial Cell Receptors and Signaling Pathways as a Nanotherapeutic Exploration against Dairy Cow Postpartum Endometritis.

Animals (Basel) 2021 May 23;11(6). Epub 2021 May 23.

Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Science, Lanzhou 730050, China.

In order to control and prevent bovine endometritis, there is a need to understand the molecular pathogenesis of the infectious disease. Bovine endometrium is usually invaded by a massive mobilization of microorganisms, especially bacteria, during postpartum dairy cows. Several reports have implicated the Gram-negative bacteria in the pathogenesis of bovine endometritis, with information dearth on the potentials of Gram-positive bacteria and their endotoxins. The invasive bacteria and their ligands pass through cellular receptors such as TLRs, NLRs, and biomolecular proteins of cells activate the specific receptors, which spontaneously stimulates cellular signaling pathways like MAPK, NF-kB and sequentially triggers upregulation of pro-inflammatory cytokines. The cascade of inflammatory induction involves a dual signaling pathway; the transcription factor NF-κB is released from its inhibitory molecule and can bind to various inflammatory genes promoter. The MAPK pathways are concomitantly activated, leading to specific phosphorylation of the NF-κB. The provision of detailed information on the molecular pathomechanism of bovine endometritis with the interaction between host endometrial cells and invasive bacteria in this review would widen the gap of exploring the potential of receptors and signal transduction pathways in nanotechnology-based drug delivery system. The nanotherapeutic discovery of endometrial cell receptors, signal transduction pathway, and cell biomolecules inhibitors could be developed for strategic inhibition of infectious signals at the various cell receptors and signal transduction levels, interfering on transcription factors activation and pro-inflammatory cytokines and genes expression, which may significantly protect endometrium against postpartum microbial invasion.
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http://dx.doi.org/10.3390/ani11061516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224678PMC
May 2021

Synergistic Effect of Dielectric Property and Energy Transfer on Charge Separation in Non-Fullerene-Based Solar Cells.

Angew Chem Int Ed Engl 2021 Jun 24;60(27):15054-15062. Epub 2021 May 24.

Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, 199 Ren'ai Road, Suzhou, 215123, P. R. China.

In non-fullerene-based photovoltaic devices, it is unclear how excitons efficiently dissociate into charge carriers under small driving force. Here, we developed a modified method to estimate dielectric constants of PM6 donor and non-fullerene acceptors. Surprisingly, most non-fullerene acceptors and blend films showed higher dielectric constants. Moreover, they exhibited larger dielectric constants differences at the optical frequency. These results are likely bound to reduced exciton binding energy and bimolecular recombination. Besides, the overlap between the emission spectrum of donor and absorption spectra of non-fullerene acceptors allowed the energy transfer from donor to acceptors. Hence, based on the synergistic effect of dielectric property and energy transfer resulting in efficient charge separation, our finding paves an alternative path to elucidate the physical working mechanism in non-fullerene-based photovoltaic devices.
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http://dx.doi.org/10.1002/anie.202103357DOI Listing
June 2021

Expression of the Testis-Specific Serine/Threonine Kinases Suggests Their Role in Spermiogenesis of Bay Scallop .

Front Physiol 2021 30;12:657559. Epub 2021 Mar 30.

MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, China.

Members of the testis-specific serine/threonine kinases (Tssk) family play critical roles in spermatogenesis in vertebrates. But in mollusks, research on Tssk family is still lagging. In this study, we systematically identified Tssk family based on the genomic and transcriptomic data from a commercially important scallop and detected the spatiotemporal expression in adult gonads. Five members were identified, with the gene length varying from 1,068 to 10,729 bp and the protein length ranging from 294 to 731 aa. All the Tssks possess a serine/threonine protein kinase catalytic (S_TKc) domain. Phylogenetic analysis revealed existence of four homologs of vertebrate Tssk1/2, Tssk3, Tssk4, Tssk5, and absence of Tssk6 in the scallop. The remaining gene (Tssk7) formed an independent clade with Tssks of other mollusks and arthropods, indicating that it may be a new member of Tssk family unique to protostomes. By investigating the expression of in four developmental stages of testes and ovaries, we found all five were primarily expressed in mature testis. hybridization experiment revealed the five were localized in the spermatids and spermatozoa. The testis-predominant expression of Tssk family suggests Tssks may play pivotal roles in spermiogenesis in the scallop. Our study provides basic information on the characteristics and expression profiles of Tssk family of . To our knowledge, it represents the first comprehensive analysis of Tssk family in mollusks.
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http://dx.doi.org/10.3389/fphys.2021.657559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042308PMC
March 2021

Asymmetric Charged Conductive Porous Films for Electricity Generation from Water Droplets Capillary Infiltrating.

ACS Appl Mater Interfaces 2021 Apr 12;13(15):17902-17909. Epub 2021 Apr 12.

Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123, China.

Hydrovoltaic devices are proposed as an alternative way to directly generate electricity due to the ubiquity of water and its interaction with specific porous structures. At present, the output power density of the reported device is limited by its low current density arising from the low surface charge density and inferior charge transport capability of the active materials. In this work, an asymmetric structure consisting of positively charged conductive polyaniline (PANI) and negatively charged TiCT MXene is proposed to build a hydrovoltaic device to achieve high conductivity and surface charge density simultaneously. An extra polyvinyl alcohol layer is utilized between PANI and MXene to reserve the asymmetric structure and maintain a constant voltage output. As a result, a peak current density of 1.8 mA/cm is achieved, which is 18 times higher than the previous peak current density of the device with an inert electrode. Our work of incorporating an asymmetric structure provides an alternative way to target high-efficiency hydrovoltaic devices with a large current density.
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http://dx.doi.org/10.1021/acsami.0c21935DOI Listing
April 2021

Bioinspired Hierarchical Nanofabric Electrode for Silicon Hydrovoltaic Device with Record Power Output.

ACS Nano 2021 Apr 9;15(4):7472-7481. Epub 2021 Apr 9.

Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123, China.

Direct electricity generation from water flow/evaporation, coined hydrovoltaic effect, has recently attracted intense interest as a facile approach to harvest green energy from ubiquitous capillary water flow or evaporation. However, the current hydrovoltaic device is inferior in output power efficiency compared to other renewable energy devices. Slow water evaporation rate and inefficient charge collection at device electrodes are two fundamental drawbacks limiting energy output efficiency. Here, we report a bioinspired hierarchical porous fabric electrode that enables high water evaporation rate, efficient charge collection, and rapid charge transport in nanostructured silicon-based hydrovoltaic devices. Such an electrode can efficiently collect charges generated in nanostructured silicon as well as induce a prompt water evaporation rate. At room temperature, the device can generate an open-circuit voltage () of 550 mV and a short-current density () of 22 μA·cm. It can output a power density over 10 μW·cm, which is 3 orders of magnitude larger than all those reported for analogous hydrovoltaic devices. Our results could supply an effective strategy for the development of high-performance hydrovoltaic devices through optimizing electrode structures.
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http://dx.doi.org/10.1021/acsnano.1c00891DOI Listing
April 2021

Sexual Development of the Hermaphroditic Scallop Revealed by Morphological, Endocrine and Molecular Analysis.

Front Cell Dev Biol 2021 16;9:646754. Epub 2021 Mar 16.

MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, China.

Simultaneous or functional hermaphrodites possessing both ovary and testis at the same time are good materials for studying sexual development. However, previous research on sex determination and differentiation was mainly conducted in gonochoristic species and studies on simultaneous hermaphrodites are still limited. In this study, we conducted a combined morphological, endocrine and molecular study on the gonadal development of a hermaphroditic scallop aged 2-10 month old. Morphological analysis showed that sex differentiation occurred at 6 months of age. By examining the dynamic changes of progesterone, testosterone and estradiol, we found testosterone and estradiol were significantly different between the ovaries and testes almost throughout the whole process, suggesting the two hormones may be involved in scallop sex differentiation. In addition, we identified two critical sex-related genes FoxL2 and Dmrt1L, and investigated their spatiotemporal expression patterns. Results showed that and were female- and male-biased, respectively, and mainly localized in the germ cells and follicular cells, indicating their feasibility as molecular markers for early identification of sex. Further analysis on the changes of and expression in juveniles showed that significant sexual dimorphic expression of occurred at 2 months of age, earlier than that of . Moreover, expression was significantly correlated with estradiol/testosterone ratio (E/T). All these results indicated that molecular sex differentiation occurs earlier than morphological sex differentiation, and may be a key driver that functions through regulating sex steroid hormones in the scallop. This study will deepen our understanding of the molecular mechanism underlying sex differentiation and development in spiralians.
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http://dx.doi.org/10.3389/fcell.2021.646754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007870PMC
March 2021

Exploring the Characteristics of Intestinal Microbiota in Hematologic Malignancy Patients via 16s rDNA High-Throughput Sequencing.

Clin Lab 2021 Feb;67(2)

Background: Malignant hematopathy is an important branch of malignant tumors, with high mortality and malignancy. The purpose of this study was to investigate the relationship between intestinal microecology and diseases by observing patients with newly diagnosed hematologic malignancy.

Methods: In this study a total of 23 stool samples were collected and analyzed, 13 of which were stool samples from newly diagnosed patients with malignant blood system diseases, and 10 were healthy individual controls. The characteristics of the intestinal flora were analyzed through 16s rDNA technology in the next-generation sequenc-ing (NGS).

Results: Bacteroidetes and Firmicutes accounted for the major abundance of the intestinal microecology in both patients with hematological malignancies and healthy controls, whereas the abundance of Bacteroidetes in the patient group was lower than that in healthy controls. Linear discriminant analysis (LDA) in LEfSa was used to search for landmark species, suggesting that Erysipelotrichi, Erysipelotrichales, and Erysipelotrichaceae could be considered as markers for patients with hematological malignant diseases. Butyricicoccus pullicaecorum, Bacteroides plebeius, and Collinsella aerofaciens also contribute to potential values as markers for intestinal flora in hematological malignant patients.

Conclusions: Patients with hematologic malignancies have altered intestinal flora structure compared with healthy individuals, which can provide new ideas for the treatment of hematologic malignancies.
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http://dx.doi.org/10.7754/Clin.Lab.2020.200448DOI Listing
February 2021

OATP1B3 Expression in Hepatocellular Carcinoma Correlates with Intralesional Gd-EOB-DTPA Uptake and Signal Intensity on Gd-EOB-DTPA-Enhanced MRI.

Cancer Manag Res 2021 9;13:1169-1177. Epub 2021 Feb 9.

Department of Radiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, People's Republic of China.

Background: To evaluate the predictive value of the OATP1B3 expression in hepatocellular carcinoma (HCC) for the gadolinium ethoxybenzyl-diethylenetriaminepentaacetic acid (Gd-EOB-DTPA) uptake and the signal intensity (SI) in the hepatobiliary (HB) phase.

Methods: In this retrospective study, we analyzed 69 liver nodules of 64 patients who underwent Gd-EOB-DTPA enhancement magnetic resonance imaging (MRI) before operation. Based on the SI in the HB phase, the patients were categorized into the hypointense HCC and iso- or hyperintense HCC groups. The OATP1B3 expression was detected by polymerase chain reaction (PCR) and immunohistochemistry. The differences between the expression of OATP1B3 and Gd-EOB-DTPA enhanced magnetic resonance imaging between the two groups of hepatocellular carcinoma were compared. The relationship between the OATP1B3 expression and the SI and relative enhancement (RE) was analyzed.

Results: The examined HCC nodules were 59 hypointense HCC and 10 iso- or hyperintense. The relative expressions of OATP1B3, HB-phase signal, and the RE of the HB phase in iso- or hyperintense were significantly higher than those of the hypointense HCC, while the RE of the HB phase increased with an increase in the OATP1B3 expression (P < 0.05).

Conclusion: The OATP1B3 expression in HCC can predict the uptake of Gd-EOB-DTPA and the SI of the HB phase. We believe that the evaluation of OATP1B3 expression will facilitate the comprehension of imaging performance of HCC in Gd-EOB-DTPA-enhanced MRI.
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http://dx.doi.org/10.2147/CMAR.S292197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882717PMC
February 2021

Structural Tunability on Naphthalimide-Based Dendrimer Gelators via Glaser Coupling Interaction with Tailored Gelation Solvent Polarity and Stimuli-Responsive Properties.

Langmuir 2021 Mar 18;37(8):2677-2682. Epub 2021 Feb 18.

College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang 050024, China.

To date, most of the low-molecular-weight gels are found serendipitously, and modification on known gelator structures via organic synthesis is an efficient methodology to prepare gel series. However, a simple, direct, and rational modification method for a known gelator is still a challenge. Herein, we employ Glaser coupling reaction to synthesize a novel dendrimer gelator BisDEC with the (ALS) structure, starting from terminal alkyne-based gelator DEC with the ALS structure. This structural change results in gels with distinct gelation solvents, mechanical properties, and stimuli-responsive abilities. The gelation abilities of DEC and BisDEC in nonpolar and polar solvents, respectively, have been examined and discussed by several experiments and Hansen constants. It is also shown that the BisDEC gel system shows intriguing self-healing, self-supporting, and grinding chromism properties.
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http://dx.doi.org/10.1021/acs.langmuir.0c03316DOI Listing
March 2021

Structural Basis of the Pore-Forming Toxin/Membrane Interaction.

Toxins (Basel) 2021 02 9;13(2). Epub 2021 Feb 9.

Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, School of Basic Medical Sciences, University of Science and Technology of China, Hefei 230027, China.

With the rapid growth of antibiotic-resistant bacteria, it is urgent to develop alternative therapeutic strategies. Pore-forming toxins (PFTs) belong to the largest family of virulence factors of many pathogenic bacteria and constitute the most characterized classes of pore-forming proteins (PFPs). Recent studies revealed the structural basis of several PFTs, both as soluble monomers, and transmembrane oligomers. Upon interacting with host cells, the soluble monomer of bacterial PFTs assembles into transmembrane oligomeric complexes that insert into membranes and affect target cell-membrane permeability, leading to diverse cellular responses and outcomes. Herein we have reviewed the structural basis of pore formation and interaction of PFTs with the host cell membrane, which could add valuable contributions in comprehensive understanding of PFTs and searching for novel therapeutic strategies targeting PFTs and interaction with host receptors in the fight of bacterial antibiotic-resistance.
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http://dx.doi.org/10.3390/toxins13020128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914777PMC
February 2021

Efficacy and safety of Reyanning mixture combined with conventional Western medicine for treating COVID-19: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jan;100(3):e24169

Pharmacy College, Shaanxi University of Chinese Medicine, Xianyang.

Background: Since its first report in December 2019, coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly emerged as a pandemic affecting nearly all countries worldwide. So far, there is no specific anti-coronavirus therapy approved for the treatment of COVID-19. In China, some traditional Chinese medicines (TCM) have been successfully applied to the treatment of SARS-CoV-2 and have achieved good clinical results, including the Reyanning mixture, but there is no systematic review about it. This study will systematically evaluate its efficacy and safety in the treatment of COVID-19.

Methods: The following electronic bibliographic databases will be searched to identify relevant studies: PubMed, MEDLINE, EMBASE, CNKI, CBM, and Wanfang databases. We will use the Cochrane Handbook for Systematic Reviews of Interventions to assess the risk of bias. The protocol will be conducted according to the approach and Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P). Manager 5.3 software and STATA 16.0 software were used to perform the meta-analysis.

Results: The systematic review and meta-analysis aims to review and pool current clinical outcomes of Reyanning mixture for the treatment of COVID-19.

Conclusion: The conclusion of this review will provide evidence to judge whether Reyanning mixture combined with Conventional Western Medicine is an effective and safe intervention for COVID-19.

Inplasy Registration Number: INPLASY2020120044.
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http://dx.doi.org/10.1097/MD.0000000000024169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837896PMC
January 2021

The therapeutic efficacy of Xuanfei Baidu Formula combined with conventional drug in the treatment of coronavirus disease 2019: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jan;100(3):e24129

Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, China.

Background: Coronavirus disease 2019 (COVID-19) is a new acute espiratory infectious disease that has been in a public health emergency of international concern. In China, the combination of Xuanfei Baidu Formula (XBF) and conventional drug is used in the clinical treatment of patients with new coronary pneumonia, However, there is no comprehensive and systematic evidence on the effectiveness and safety of XBF.

Materials And Methods: We search for research in PubMed, China National Knowledge Infrastructure, Wan-fang Database, China Biomedical Database, and Chinese Science Citation Database. For "Xuanfei Baidu Formula" and "COVID-19," we screened suitable articles without language restrictions on keywords, Review Manager 5.3 and STATA 14.2 software was used for the data analysis.

Results: The systematic review and meta-analysis will evaluate the efficacy and safety of XBF combined with conventional drug in the treatment of COVID-19.

Conclusion: We will provide evidence of XBF for the treatment on COVID-19 patients.

Inplasy Registration Number: INPLASY2020120011.
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http://dx.doi.org/10.1097/MD.0000000000024129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837860PMC
January 2021

Traditional Chinese medicine Lianhua Qingwen for treating COVID-19: A protocol for a meta-analysis and systematic review of randomized controlled trials.

Medicine (Baltimore) 2021 Jan;100(2):e24204

Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi, China.

Background: Since the outbreak of coronavirus disease 2019 (COVID-19) in 2019, it has swept the world with rapid development and is one of the infectious diseases that seriously threatened global public health. Because of the complex pathogenesis, high infectivity, and high fatality rate of COVID-19, there are no effective treatments for this epidemic at present. Traditional Chinese Medicine (TCM) has a long clinical history in the prevention and treatment of such acute infectious diseases. The therapeutic effect of Lianhua Qingwen (LHQW) on this new coronary pneumonia has attracted the attention of all walks of life, and relevant research reports continue to appear. Here, we intend to conduct a systematic review and meta-analysis of randomized controlled trials (RCT) to evaluate the efficacy of LHQW in COVID-19 patients.

Methods: We will search each database from the built-in until Dec 2020. The English literature mainly search the Cochrane Library, EMBASE, PubMed, and Web of Science, while the Chinese literature come from CNKI, VIP, Chinese Biomedical Database (CBM), Chinese Science Citation Database (CSCD), and Wan Fang database. Simultaneously, we will retrieve clinical registration tests. This study only screens the RCT of LHQW against COVID-19 and evaluates its efficacy and safety. We will use the Cochrane Handbook to systematically review interventions to assess the risk of bias. The protocol will be reported according to the approach and preferred report items for systematic review and meta-analysis protocols (PRISMA - P). Finally, RevMan software version 5.3 will be used for meta-analysis.

Results: The systematic review and meta-analysis aim to review and pool current clinical outcomes of LHQW for treating COVID-19.

Conclusion: This study will provide further evidence for the efficacy and safety of LHQW in the treatment of COVID-19.

Inplasy Registration Number: INPLASY2020120043.
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http://dx.doi.org/10.1097/MD.0000000000024204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7808524PMC
January 2021

MicroRNAome: Potential and Veritable Immunomolecular Therapeutic and Diagnostic Baseline for Lingering Bovine Endometritis.

Front Vet Sci 2020 23;7:614054. Epub 2020 Dec 23.

Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Science, Lanzhou, China.

The bovine endometrium is a natural pathogen invasion barrier of the uterine tissues' endometrial epithelial cells that can resist foreign pathogen invasion by controlling the inflammatory immune response. Some pathogens suppress the innate immune system of the endometrium, leading to prolonged systemic inflammatory response through the blood circulation or cellular degradation resulting in bovine endometritis by bacterial endotoxins. The microRNA (miRNA) typically involves gene expression in multicellular organisms in post-transcription regulation by affecting both the stability and the translation of messenger RNA. Accumulated evidence suggests that miRNAs are important regulators of genes in several cellular processes. They are a class of endogenous non-coding RNAs, which play pivotal roles in the inflammatory response of reproductive diseases. Studies confirmed that miRNAs play a key regulatory role in various inflammatory diseases by mediating the molecular mechanism of inflammatory cytokines signal pathways. It implicates some miRNAs in the occurrence of bovine endometritis, resorting to regulating the activities of some inflammatory cytokines, chemokine, differentially expressed genes, and protein through modulating of specific cellular signal pathways functions. This review dwells on improving the knowledge of the role of miRNAs involvement in inflammatory response as to early diagnosis, control, and prevention of bovine endometritis and consequently enlighten on the molecular improvement of the genes coded by various differentially expressed miRNA through the need to adopt recent genetic technologies and the development of new pharmaceutical preparations.
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http://dx.doi.org/10.3389/fvets.2020.614054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785807PMC
December 2020

Competition between two phosphatases fine-tunes Hedgehog signaling.

J Cell Biol 2021 Feb;220(2)

Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing, China.

Hedgehog (Hh) signaling is essential for embryonic development and adult homeostasis. How its signaling activity is fine-tuned in response to fluctuated Hh gradient is less known. Here, we identify protein phosphatase V (PpV), the catalytic subunit of protein phosphatase 6, as a homeostatic regulator of Hh signaling. PpV is genetically upstream of widerborst (wdb), which encodes a regulatory subunit of PP2A that modulates high-level Hh signaling. We show that PpV negatively regulates Wdb stability independent of phosphatase activity of PpV, by competing with the catalytic subunit of PP2A for Wdb association, leading to Wdb ubiquitination and subsequent proteasomal degradation. Thus, regulated Wdb stability, maintained through competition between two closely related phosphatases, ensures graded Hh signaling. Interestingly, PpV expression is regulated by Hh signaling. Therefore, PpV functions as a Hh activity sensor that regulates Wdb-mediated PP2A activity through feedback mechanisms to maintain Hh signaling homeostasis.
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http://dx.doi.org/10.1083/jcb.202010078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774589PMC
February 2021

Polymerization inspired synthesis of [email protected] nanowires with long cycling stability for lithium ion battery anodes: growth mechanism and electrochemical performance.

Dalton Trans 2021 Jan;50(2):535-545

Hunan Provincial Key Laboratory of Efficient and Clean Utilization of Manganese Resources, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, Hunan, China.

Manganese-based transition metal oxides are regarded as one kind of high capacity and low cost anode material for Li-ion batteries. To overcome the challenges of poor electrical conductivity and large volumetric expansion during the charging-discharging process of MnO, we here synthesize [email protected] ([email protected]) nanowires via the polymerization inspired in situ growth of [Mn-NTA] (NTA = nitrilotriacetic acid) precursor nanowires with a subsequent heat treatment process. The growth mechanism of [Mn-NTA] precursor nanowires was studied. The morphology of the precursor nanowires depended largely on the molar ratio of MnCl2 to NTA reactants. At a molar ratio of 2, the length of the [Mn-NTA] nanowires reached up to more than 140 μm. Furthermore, the as-synthesized [email protected] nanowires were integrated with a very low content of reduced graphene oxide (rGO) to prepare a self-standing paper-like [email protected]/rGO anode for lithium ion batteries without a binder. The [email protected]/rGO anode showed a unique structure with one-dimensional porous MnO nanowires hierarchically encapsulated by a conductive carbon framework. As a result, the self-standing electrode achieved a high capacity of 1368 mA h g-1 after 100 cycles at a current density of 100 mA g-1 and prominent cycling stability with a capacity of 689.9 mA h g-1 even after 1700 cycles at 2000 mA g-1.
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http://dx.doi.org/10.1039/d0dt03540hDOI Listing
January 2021

Insights into the photocatalysis mechanism of the novel 2D/3D Z-Scheme g-CN/SnS heterojunction photocatalysts with excellent photocatalytic performances.

J Hazard Mater 2021 Jan 20;402:123711. Epub 2020 Aug 20.

Department of Mechanical Engineering, The University of Hong Kong, Hong Kong, China. Electronic address:

A novel 2D/3D Z-scheme g-CN/SnS photocatalyst was successfully fabricated via self-assembly forming 3D flower-like SnS microspheres on the surface of the 2D g-CN nanosheets. The photocatalytic performances of the samples were systematically explored through catalytic reduction of Cr and oxidation of Bisphenol S (BPS) under the illumination of visible light, and the photocatalytic degradation pathway of BPS was also proposed based on the degradation products confirmed by GCMS. Among the as-prepared samples, 0.4-g-CN/SnS exhibited the most efficient photocatalytic performances, and the apparent quantum efficiency (QE) for the removal of Cr could achieve 30.3 %, which is 2.8 times higher than that of the SnS. The enhancing photocatalytic activities originated from the efficient interfacial charge migration and separation obtained in g-CN/SnS, which was firstly verified via the photoluminescence spectra, time-resolved photoluminescence spectra and photoelectrochemical characterizations. Importantly, the DFT calculated shows that the band distribution of the g-CN/SnS sample is staggered near the forbidden, which can facilitate the efficient interfacial charge migration and separation as well as result in the improvement of the catalytic activity. Finally, we put forward a more reasonable Z-scheme charge transfer mechanism, it was verified by analysing the results of free radical scavenging tests, EPR experiments and theoretical calculations.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123711DOI Listing
January 2021

The lncRNA H19 alleviates muscular dystrophy by stabilizing dystrophin.

Nat Cell Biol 2020 11 26;22(11):1332-1345. Epub 2020 Oct 26.

Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Dystrophin proteomic regulation in muscular dystrophies (MDs) remains unclear. We report that a long noncoding RNA (lncRNA), H19, associates with dystrophin and inhibits E3-ligase-dependent polyubiquitination at Lys 3584 (referred to as Ub-DMD) and its subsequent protein degradation. In-frame deletions in BMD and a DMD non-silent mutation (C3340Y) resulted in defects in the ability of the protein to interact with H19, which caused elevated Ub-DMD levels and dystrophin degradation. Dmd C3333Y mice exhibited progressive MD, elevated serum creatine kinase, heart dilation, blood vessel irregularity and respiratory failure with concurrently reduced dystrophin and increased Ub-DMD status. H19 RNA oligonucleotides conjugated with agrin (AGR-H19) and nifenazone competed with or inhibited TRIM63. Dmd C3333Y animals, induced-pluripotent-stem-cell-derived skeletal muscle cells from patients with Becker MD and mdx mice subjected to exon skipping exhibited inhibited dystrophin degradation, preserved skeletal and cardiac muscle histology, and improved strength and heart function following AGR-H19 or nifenazone treatment. Our study paves the way for meaningful targeted therapeutics for Becker MD and for certain patients with Duchenne MD.
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http://dx.doi.org/10.1038/s41556-020-00595-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7951180PMC
November 2020

Suppression of Inflammation Delays Hair Cell Regeneration and Functional Recovery Following Lateral Line Damage in Zebrafish Larvae.

Biomolecules 2020 10 16;10(10). Epub 2020 Oct 16.

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, Division of Biomedical Sciences, University of Science and Technology of China, Hefei 230027, China.

Cochlear hair cells in human beings cannot regenerate after loss; however, those in fish and other lower species can. Recently, the role of inflammation in hair cell regeneration has been attracting the attention of scientists. In the present study, we investigated how suppression of inflammatory factors affects hair cell regeneration and the functional recovery of regenerated hair cells in zebrafish. We killed hair cells in the lateral line of zebrafish larvae with CuSO to induce an inflammatory response and coapplied BRS-28, an anti-inflammatory agent to suppress the inflammation. The recovery of the hair cell number and rheotaxis was slower when CuSO and BRS-28 were coapplied than when CuSO was applied alone. The recovery of hair cell count lagged behind that of the calcium imaging signal during the regeneration. The calcium imaging signal in the neuromasts in the inflammation-inhibited group was weaker than that in the noninflammation-inhibited group at the early stage of regeneration, although it returned to normal at the late stage. Our study demonstrates that suppressing inflammation by BRS-28 delays hair cell regeneration and functional recovery when hair cells are damaged. We suspect that BRS-28 inhibits pro-inflammatory factors and thereby reduces the migration of macrophages to delay the regeneration of hair cells.
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http://dx.doi.org/10.3390/biom10101451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650643PMC
October 2020

A Network Pharmacological Approach to Investigate the Mechanism of Action of Active Ingredients of Epimedii Herba and Their Potential Targets in Treatment of Alzheimer's Disease.

Med Sci Monit 2020 Sep 27;26:e926295. Epub 2020 Sep 27.

Department of Histology and Embryology, School of Basic Medical Sciences, Southwest Medical University, Luzhou, Sichuan, China (mainland).

BACKGROUND Epimedii Herba is a traditional Chinese herbal medicine used to treat central nervous system diseases such as Alzheimer's disease in China. However, the pharmacological mechanism is unclear. To investigate the mechanisms of Epimedii Herba in the treatment of Alzheimer's disease, we assessed effective compounds, corresponding targets, and related pathways of Epimedii Herba in the treatment of Alzheimer's disease based on network pharmacology. MATERIAL AND METHODS The active components and targets of Epimedii Herba were obtained through the TCMSP database and the DrugBank database. The DisGeNET database and GeneCards database were used to search for Alzheimer's disease targets. The common targets of components and disease were obtained by Wayne diagram. Gene ontology (GO) analysis and enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed using the DAVID database. The component-target-pathway interaction network model was constructed using Cytoscape software. Auto Duck Vina software was used for molecular docking to analyze the affinity of the key ingredients and the main targets. RESULTS We screened 17 active ingredients and 27 key targets of Epimedii Herba in the treatment of Alzheimer's disease, which were related to the HIF-1 signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, NF-kappaB signaling pathway, VEGF signaling pathway, and sphingolipid signaling pathway. CONCLUSIONS Based on network pharmacology, the multi-component, multi-target, and multi-pathway characteristics of Epimedii Herba in the treatment of Alzheimer's disease were explored. Our results provide new ideas for future pharmacological and experimental research on Epimedii Herba in the treatment of Alzheimer's disease.
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http://dx.doi.org/10.12659/MSM.926295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528617PMC
September 2020

Potential GnRH and steroidogenesis pathways in the scallop Patinopecten yessoensis.

J Steroid Biochem Mol Biol 2020 11 3;204:105756. Epub 2020 Oct 3.

MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, 266003, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.

Gonadotropin-releasing hormone (GnRH) controls synthesis of sex steroid hormones through hypothalamic-pituitary-gonadal (HPG) axis in vertebrates. But in mollusks, research on GnRH and steroidogenesis pathways is still limited. In this study, we first identified two gonadotropin receptor like genes (LGR and LGR5L) and four steroidogenesis-related genes (CYP17A, HSD17B12, HSD3B1 and HSD3B2) in the scallop Patinopecten yessoensis. By examining the expression of 11 genes in the ganglia and/or gonad as well as the concentration of progesterone, testosterone and estradiol in the gonad, we postulate that a potential GnRH signaling pathway (GnRH-GnRHR-GPB5-LGR/LGR5L) in the cerebral and pedal ganglia (CPG) and steroidogenesis pathway (CYP17A, HSD17B12 and HSD3B1) in the gonad are involved in regulating sex steroid hormones. E/T index that indicates aromatase activity is higher in the ovary than testis and is positively correlated with the expression of FOXL2 in the gonad, implying the presence of aromatase in the scallop. In addition, we confirmed that expression of most of the downstream genes in the two pathways was significantly elevated after injection of mature py-GnRH peptide. This study would contribute to a new understanding of the molecular basis underlying reproduction regulation by GnRH in mollusks.
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http://dx.doi.org/10.1016/j.jsbmb.2020.105756DOI Listing
November 2020

Effect of ethylparaben on the growth and development of Drosophila melanogaster on preadult.

Environ Toxicol Pharmacol 2020 Nov 16;80:103495. Epub 2020 Sep 16.

College of Life Sciences, Shaanxi Normal University, No. 620, West Chang'an Avenue, Chang'an District, Xi'an, 710119, China. Electronic address:

Parabens are esters of p-hydroxybenzoic acid, including methylparaben (MP), ethylparaben (EP), propylparaben (PP), and the like. This substance has estrogenic and antiandrogenic effects, and a putative role in promoting cancer through endocrine disruption. By exposing Drosophila melanogaster to different concentrations of EP (300 mg/L, 700 mg/L, and 1000 mg/L), we investigated the effect of EP on the growth and development of D. melanogaster before emergence. We found that EP prolonged the development cycle of D. melanogaster, and changed the relative expression levels of Met, Gce, EcR, Kr-h1, and Br. In addition, EP reduced the titer of juvenile hormone Ⅲ (JH Ⅲ) and 20-hydroxyecdysone (20E), and delayed the peak of hormone secretion. This study provided a more objective and thorough assessment of safety for the parabens.
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http://dx.doi.org/10.1016/j.etap.2020.103495DOI Listing
November 2020

Grapevine improves drought resistance by directly activating and promoting lignin biosynthesis through the regulation of three peroxidase genes.

Hortic Res 2020 1;7:150. Epub 2020 Sep 1.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100 Shaanxi China.

Drought stress severely affects grapevine quality and yield, and recent reports have revealed that lignin plays an important role in protection from drought stress. Since little is known about lignin-mediated drought resistance in grapevine, we investigated its significance. Herein, we show that mediates drought resistance by activating the expression of lignin biosynthetic genes and increasing lignin deposition. Transgenic grapevine plants overexpressing exhibited lignin deposition (mainly G and S monomers) in the stem secondary xylem under control conditions, which resulted from the upregulated expression of and . Overexpression of improves drought tolerance, characterized by a reduction in the water loss rate, maintenance of an effective photosynthesis rate, and increased lignin content (mainly G monomer) in leaves under drought conditions. Electrophoretic mobility shift assay, luciferase reporter assays, and chromatin immunoprecipitation-qPCR assays indicated that directly binds to the G-box -element in the promoters of lignin biosynthetic () and drought-responsive () genes to regulate their expression. In summary, we report a novel -mediated mechanism linking lignification and drought tolerance in grapevine. The results of this study may be of value for the development of molecular breeding strategies to produce drought-resistant fruit crops.
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http://dx.doi.org/10.1038/s41438-020-00372-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7458916PMC
September 2020

Genome of Tetraploid Fish Schizothorax o'connori Provides Insights into Early Re-diploidization and High-Altitude Adaptation.

iScience 2020 Aug 22;23(9):101497. Epub 2020 Aug 22.

Institute of Fisheries Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, China. Electronic address:

Whole-genome duplications (WGDs) of Schizothoracinae are believed to have played a significant role in speciation and environmental adaptation on the Qinghai-Tibet Plateau (QTP). Here, we present a genome for Schizothorax o'connori, a QTP endemic fish and showed the species as a young tetraploid with a recent WGD later than ∼1.23 mya. We exhibited that massive insertions between duplicated genomes caused by transposon bursts could induce mutagenesis in adjacent sequences and alter the expression of neighboring genes, representing an early re-diploidization process in a polyploid genome after WGD. Meanwhile, we found that many genes involved in DNA repair and folate transport/metabolism experienced natural selection and might contribute to the environmental adaptation of this species. Therefore, the S. o'connori genome could serve as a young tetraploid model for investigations of early re-diploidization in polyploid genomes and offers an invaluable genetic resource for environmental adaptation studies of the endemic fish of the QTP.
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http://dx.doi.org/10.1016/j.isci.2020.101497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7486454PMC
August 2020