Publications by authors named "Yair Glick"

42 Publications

Hidden in Plain Sight: Eosinophilic Fasciitis.

Am J Med 2022 Jul 9. Epub 2022 Jul 9.

Chief Consultant, Meuhedet HMO, Rehovot, Jerusalem, Israel; Faculty of Medicine, Hadassah Medical School, Hebrew University, Jerusalem, Israel; Consultant, Department of Imaging, Laniado Hospital, Sanz Medical Center, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2022.06.011DOI Listing
July 2022

All That Wheezes Is Not Asthma: Giant Hiatal Hernia.

Am J Med 2022 Jun 6. Epub 2022 Jun 6.

Department Imaging, Laniado Hospital, Sanz Medical Centre, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2022.05.030DOI Listing
June 2022

Diffuse Large B-cell Lymphoma Presenting with Paraparesis.

QJM 2022 May 24. Epub 2022 May 24.

Departments of Medicine, (AS, ID), and Imaging, (YG), Laniado Hospital, Sanz Medical Centre, Netanya; Department of Radiology, Tel Aviv Sourasky Medical Center, (MI); and the Faculty of Medicine, Hebrew University Hadassah, (AS), Jerusalem, Israel.

The development of paraparesis in the setting of back pain and lassitude in an immunocompetent patient was diagnosed as the presenting feature of diffuse large B-cell lymphoma. MRI imaging and surgical biopsy were diagnostic, despite the absence of lymphadenopathy or splenomegaly, and near-normal laboratory workup. Different possible pathological mechanisms of paraparesis in large B-cell lymphoma are discussed.
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http://dx.doi.org/10.1093/qjmed/hcac132DOI Listing
May 2022

Cerebral Sinus Vein Thrombosis as Presenting Feature of Adenocarcinoma of the Lung.

Am J Med 2022 03 23;135(3):e65-e66. Epub 2021 Nov 23.

Department of Imaging, Laniado Hospital, Sanz Medical Centre, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2021.11.001DOI Listing
March 2022

Caveat: Elusive Foreign Body: A Reminder.

Am J Med 2022 02 13;135(2):e51-e52. Epub 2021 Oct 13.

Department of Medicine, Laniado Hospital, Sanz Medical Centre, Netanya, Israel; Faculty of Medicine, Hebrew University Hadassah Medical School, Jerusalem, Israel. Electronic address:

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http://dx.doi.org/10.1016/j.amjmed.2021.09.009DOI Listing
February 2022

Not What It Seems: Obstructive Jaundice in an HIV-Positive Patient.

Am J Med 2021 11 29;134(11):e550-e551. Epub 2021 Jun 29.

Department of Imaging, Laniado Hospital, Sanz Medical Center, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2021.05.020DOI Listing
November 2021

Hemoptysis in a cannabis user.

Am J Emerg Med 2021 Nov 4;49:442.e1-442.e2. Epub 2021 May 4.

Department of Imaging, Sanz Medical Center, Netanya, Israel.

A healthy young man presented to the emergency department with mild hemoptysis associated with cannabis abuse. He was on no medications and cocaine abuse was ruled out by both history and negative toxicology screens. There were neither signs of an infection, nor of a systemic or cardiac disease. Imaging studies were consistent with diffuse alveolar hemorrhage, a reported, albeit rare adverse drug reaction of tetrahydrocannabinol (Naranjo score 6). The patient improved spontaneously within a few days, hemoptysis stopped and repeat imaging was entirely normal. With the increase in cannabis abuse and enhanced cannabis potency worldwide clinicians may increasingly encounter even unusual cannabis-associated adverse drug reactions, including associated diffuse alveolar hemorrhage.
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http://dx.doi.org/10.1016/j.ajem.2021.05.003DOI Listing
November 2021

Bartonella and the Spleen.

Am J Med 2021 08 31;134(8):e461-e462. Epub 2021 Mar 31.

Department of Medicine, Laniado Hospital, Sanz Medical Center, Netanya, Israel; Faculty of Medicine, Hebrew University and Hadassah Medical School, Jerusalem, Israel. Electronic address:

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http://dx.doi.org/10.1016/j.amjmed.2021.02.018DOI Listing
August 2021

Subclavian Steal Syndrome, Cerebellar Infarctions, and Alcohol.

Am J Med 2021 05 27;134(5):e317-e318. Epub 2020 Oct 27.

Department of Imaging, Laniado Hospital, Sanz Medical Center, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2020.09.037DOI Listing
May 2021

Pauci-Symptomatic Aortic Dissection.

Am J Med 2021 03 28;134(3):e201-e202. Epub 2020 Sep 28.

Department of Imaging, Laniado Hospital, Sanz Medical Centre, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2020.08.036DOI Listing
March 2021

Opioid analgesics, faecal stones and rectosigmoid perforation.

Postgrad Med J 2021 Dec 29;97(1154):760-761. Epub 2020 Sep 29.

Department of Imaging, Laniado Hospital, Sanz Medical Centre, Netanya, Israel.

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http://dx.doi.org/10.1136/postgradmedj-2020-138960DOI Listing
December 2021

Acute painless pancreatitis as an unusual presentation of leptospirosis in a low-incidence country.

BMJ Case Rep 2020 Aug 24;13(8). Epub 2020 Aug 24.

Laniado Hospital, Sanz Medical Centre, Netanya, Israel.

A healthy, urban-dwelling man presented with lassitude, jaundice without increased liver enzymes or obstructive features on imaging, brief acute kidney injury, leucocytosis with near-normal C reactive protein and markedly increased serum amylase and lipase. Leptospirosis was not considered for 10 days because of the low incidence of the disease in the country, absent animal contact and physicians' low index of suspicion. Presentation without fever and without the commonly associated abdominal pain, myalgia, headache, thrombocytopaenia or elevated serum creatine kinase added to the diagnostic challenge. Once an infectious cause of acute pancreatitis was contemplated, leptospirosis was immediately sought and diagnosed by PCR of urine and microscopic agglutination test, and he fully recovered on ceftriaxone. Physicians in countries with a low incidence of leptospirosis should be more aware of the possibility of the disease even when several key features such as fever or pain are missing and the patient has a rare infectious acute pancreatitis.
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http://dx.doi.org/10.1136/bcr-2020-234988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7449284PMC
August 2020

Multiple Spontaneous Osteoporotic Fractures Mimicking Metastatic Bone Disease.

Am J Med Sci 2020 09 27;360(3):309-311. Epub 2020 May 27.

Department of Imaging, Laniado Hospital, Sanz Medical Centre, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjms.2020.05.027DOI Listing
September 2020

Coma During a Febrile Illness - Respiratory Syncytial Virus-Triggered Ischemic Stroke.

Am J Med 2020 09 19;133(9):e521-e523. Epub 2020 May 19.

Department of Medicine, Laniado Hospital, Sanz Medical Center, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2020.03.060DOI Listing
September 2020

An Underappreciated Embolic Source: Aortic Arch Floating Thrombus.

Am J Med 2020 08 17;133(8):e430-e431. Epub 2020 Feb 17.

Department of Medicine, Laniado Hospital, Sanz Medical Centre, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2020.01.021DOI Listing
August 2020

Acute extensive myocardial calcification.

Postgrad Med J 2020 Nov 5;96(1141):719. Epub 2020 Jan 5.

Department of Internal Medicine, Laniado Hospital, Sanz Medical Centre, Netanya, Israel

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http://dx.doi.org/10.1136/postgradmedj-2019-137211DOI Listing
November 2020

SIRT6 Promotes Hepatic Beta-Oxidation via Activation of PPARα.

Cell Rep 2019 12;29(12):4127-4143.e8

Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan 5290002, Israel. Electronic address:

The pro-longevity enzyme SIRT6 regulates various metabolic pathways. Gene expression analyses in SIRT6 heterozygotic mice identify significant decreases in PPARα signaling, known to regulate multiple metabolic pathways. SIRT6 binds PPARα and its response element within promoter regions and activates gene transcription. Sirt6 results in significantly reduced PPARα-induced β-oxidation and its metabolites and reduced alanine and lactate levels, while inducing pyruvate oxidation. Reciprocally, starved SIRT6 transgenic mice show increased pyruvate, acetylcarnitine, and glycerol levels and significantly induce β-oxidation genes in a PPARα-dependent manner. Furthermore, SIRT6 mediates PPARα inhibition of SREBP-dependent cholesterol and triglyceride synthesis. Mechanistically, SIRT6 binds PPARα coactivator NCOA2 and decreases liver NCOA2 K780 acetylation, which stimulates its activation of PPARα in a SIRT6-dependent manner. These coordinated SIRT6 activities lead to regulation of whole-body respiratory exchange ratio and liver fat content, revealing the interactions whereby SIRT6 synchronizes various metabolic pathways, and suggest a mechanism by which SIRT6 maintains healthy liver.
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http://dx.doi.org/10.1016/j.celrep.2019.11.067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7165364PMC
December 2019

Cytomegalovirus-Associated Splenic Infarction.

Am J Med 2020 03 14;133(3):e104-e105. Epub 2019 Oct 14.

Department of Imaging, Laniado Hospital, Sanz Medical Center, Netanya, Jerusalem, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2019.09.011DOI Listing
March 2020

Exclusive Temporal Stimulation of IL-10 Expression in LPS-Stimulated Mouse Macrophages by cAMP Inducers and Type I Interferons.

Front Immunol 2019 6;10:1788. Epub 2019 Aug 6.

Department of Biochemistry & Molecular Biology, School of Neurobiology, Biochemistry & Biophysics, Tel Aviv University, Tel Aviv, Israel.

Expression of the key anti-inflammatory cytokine IL-10 in lipopolysaccharide (LPS)-stimulated macrophages is mediated by a delayed autocrine/paracrine loop of type I interferons (IFN) to ensure timely attenuation of inflammation. We have previously shown that cAMP synergizes with early IL-10 expression by LPS, but is unable to amplify the late type I IFN-dependent activity. We now examined the mechanism of this synergistic transcription in mouse macrophages at the promoter level, and explored the crosstalk between type I IFN signaling and cAMP, using the β-adrenergic receptor agonist, isoproterenol, as a cAMP inducer. We show that silencing of the type I IFN receptor enables isoproterenol to synergize with LPS also at the late phase, implying that autocrine type I IFN activity hinders synergistic augmentation of LPS-stimulated IL-10 expression by cAMP at the late phase. Furthermore, IL-10 expression in LPS-stimulated macrophages is exclusively stimulated by either IFNα or isoproterenol. We identified a set of two proximate and inter-dependent cAMP response element (CRE) sites that cooperatively regulate early IL-10 transcription in response to isoproterenol-stimulated CREB and that further synergize with a constitutive Sp1 site. At the late phase, up-regulation of Sp1 activity by LPS-stimulated type I IFN is correlated with loss of function of the CRE sites, suggesting a mechanism for the loss of synergism when LPS-stimulated macrophages switch to type I IFN-dependent IL-10 expression. This report delineates the molecular mechanism of cAMP-accelerated IL-10 transcription in LPS-stimulated murine macrophages that can limit inflammation at its onset.
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http://dx.doi.org/10.3389/fimmu.2019.01788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691811PMC
October 2020

Splenic Vein Thrombosis-Caught in the Middle.

Am J Med 2020 02 13;133(2):e48-e49. Epub 2019 Aug 13.

Department of Imaging, Laniado Hospital, Sanz Medical Centre, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2019.07.035DOI Listing
February 2020

A Reminder From the Past: Unsuspected Splenic Calcified Granulomatosis Decades After Pulmonary Tuberculosis.

Am J Med 2019 12 24;132(12):e848-e849. Epub 2019 Jun 24.

Ramat Aviv Medical Center, Tel Aviv, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2019.05.053DOI Listing
December 2019

A Fateful Miss: Ground-Level Fall Revealing Occult Cervical Spinal Stenosis.

Am J Med 2019 06 27;132(6):e581-e582. Epub 2019 Feb 27.

Department of Medicine, Laniado Hospital, Sanz Medical Centre, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2019.02.003DOI Listing
June 2019

A high-throughput integrated microfluidics method enables tyrosine autophosphorylation discovery.

Commun Biol 2019 30;2:42. Epub 2019 Jan 30.

The Mina and Everard Goodman Faculty of Life Sciences and the Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Building #206, Ramat-Gan, 5290002 Israel.

Autophosphorylation of receptor and non-receptor tyrosine kinases is a common molecular switch with broad implications for pathogeneses and therapy of cancer and other human diseases. Technologies for large-scale discovery and analysis of autophosphorylation are limited by the inherent difficulty to distinguish between phosphorylation and autophosphorylation in vivo and by the complexity associated with functional assays of receptors kinases in vitro. Here, we report a method for the direct detection and analysis of tyrosine autophosphorylation using integrated microfluidics and freshly synthesized protein arrays. We demonstrate the efficacy of our platform in detecting autophosphorylation activity of soluble and transmembrane tyrosine kinases, and the dependency of in vitro autophosphorylation assays on membranes. Our method, Integrated Microfluidics for Autophosphorylation Discovery (IMAD), is high-throughput, requires low reaction volumes and can be applied in basic and translational research settings. To our knowledge, it is the first demonstration of posttranslational modification analysis of membrane protein arrays.
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http://dx.doi.org/10.1038/s42003-019-0286-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353932PMC
April 2020

Postpartum shoulder pain and shortness of breath.

Postgrad Med J 2019 Apr 23;95(1122):221-222. Epub 2019 Jan 23.

Department of Medicine, Laniado Hospital, Sanz Medical Centre, Netanya, Israel

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http://dx.doi.org/10.1136/postgradmedj-2018-136224DOI Listing
April 2019

Ruptured Urinary Bladder After a 'Simple' Fall.

Am J Med 2019 01 23;132(1):e11-e12. Epub 2018 Aug 23.

Department of Imaging, Laniado Hospital, Sanz Medical Center, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2018.08.010DOI Listing
January 2019

A Dangerous Entrapment: Pelvic Aneurysms Causing Hydronephrosis.

Am J Med 2018 11 1;131(11):e467-e468. Epub 2018 Aug 1.

Department of Imaging, Laniado Hospital, Sanz Medical Center, Netanya, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2018.07.022DOI Listing
November 2018

Prostate Abscesses and Staphylococcus aureus Bacteremia.

Am J Med 2018 10 18;131(10):e417-e418. Epub 2018 Jun 18.

Department of Medicine, Laniado Hospital-Sanz Medical Center, Netanya, Israel; Faculty of Medicine, Hebrew University-Hadassah Medical School, Jerusalem, Israel.

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http://dx.doi.org/10.1016/j.amjmed.2018.05.031DOI Listing
October 2018

Neuregulin 1 discovered as a cleavage target for the HCV NS3/4A protease by a microfluidic membrane protein array.

N Biotechnol 2018 Oct 10;45:113-122. Epub 2018 Feb 10.

Mina and Everard Goodman Faculty of Life Sciences and Bar Ilan Institute for Nanotechnology and Advanced Materials, Anna Web Nanotechnology bld. (206), Bar Ilan University, Ramat Gan, 5290002, Israel. Electronic address:

The hepatitis C virus (HCV) non-structural protein 3 (NS3) is essential for HCV maturation. The NS3/4A protease is a target for several HCV treatments and is a well-known target for HCV drug discovery. The protein is membrane associated and thus probably interacts with other membrane proteins. However, the vast majority of known NS3 host partners are soluble proteins rather than membrane proteins, most likely due to lack of appropriate platforms for their discovery. Utilization of an integrated microfluidics platform enables analysis of membrane proteins in their native form. We screened over 2800 membrane proteins for interaction with NS3 and 90 previously unknown interactions were identified. Of these, several proteins were selected for validation by co-immunoprecipitation and for NS3 proteolytic activity. Bearing in mind the considerable number of interactions formed, together with the popularity of NS3/4A protease as a drug target, it was striking to note its lack of proteolytic activity. Only a single protein, Neuregulin1, was observed to be cleaved, adding to the 3 known NS3/4A cleavage targets. Neuregulin1 participates in neural proliferation. Recent studies have shown its involvement in HCV infection and hepatocellular carcinoma. We showed that NS3/4A triggers an increase in neuregulin1 mRNA levels in HCV infected cells. Despite this increase, its protein concentration is decreased due to proteolytic cleavage. Additionally, its EGF-like domain levels were increased, possibly explaining the ErbB2 and EGFR upregulation in HCV infected cells. The newly discovered protein interactions may provide insights into HCV infection mechanisms and potentially provide new therapeutic targets against HCV.
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http://dx.doi.org/10.1016/j.nbt.2018.02.004DOI Listing
October 2018

DNA Bipedal Motor Achieves a Large Number of Steps Due to Operation Using Microfluidics-Based Interface.

ACS Nano 2017 04 14;11(4):4002-4008. Epub 2017 Apr 14.

Department of Chemistry and the Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev , Beer Sheva, 84105, Israel.

Realization of bioinspired molecular machines that can perform many and diverse operations in response to external chemical commands is a major goal in nanotechnology, but current molecular machines respond to only a few sequential commands. Lack of effective methods for introduction and removal of command compounds and low efficiencies of the reactions involved are major reasons for the limited performance. We introduce here a user interface based on a microfluidics device and single-molecule fluorescence spectroscopy that allows efficient introduction and removal of chemical commands and enables detailed study of the reaction mechanisms involved in the operation of synthetic molecular machines. The microfluidics provided 64 consecutive DNA strand commands to a DNA-based motor system immobilized inside the microfluidics, driving a bipedal walker to perform 32 steps on a DNA origami track. The microfluidics enabled removal of redundant strands, resulting in a 6-fold increase in processivity relative to an identical motor operated without strand removal and significantly more operations than previously reported for user-controlled DNA nanomachines. In the motor operated without strand removal, redundant strands interfere with motor operation and reduce its performance. The microfluidics also enabled computer control of motor direction and speed. Furthermore, analysis of the reaction kinetics and motor performance in the absence of redundant strands, made possible by the microfluidics, enabled accurate modeling of the walker processivity. This enabled identification of dynamic boundaries and provided an explanation, based on the "trap state" mechanism, for why the motor did not perform an even larger number of steps. This understanding is very important for the development of future motors with significantly improved performance. Our universal interface enables two-way communication between user and molecular machine and, relying on concepts similar to that of solid-phase synthesis, removes limitations on the number of external stimuli. This interface, therefore, is an important step toward realization of reliable, processive, reproducible, and useful externally controlled DNA nanomachines.
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http://dx.doi.org/10.1021/acsnano.7b00547DOI Listing
April 2017

Control and automation of multilayered integrated microfluidic device fabrication.

Lab Chip 2017 01;17(3):557-566

Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar Ilan University, Ramat Gan, Israel.

Integrated microfluidics is a sophisticated three-dimensional (multi layer) solution for high complexity serial or parallel processes. Fabrication of integrated microfluidic devices requires soft lithography and the stacking of thin-patterned PDMS layers. Precise layer alignment and bonding is crucial. There are no previously reported standards for alignment of the layers, which is mostly performed using uncontrolled processes with very low alignment success. As a result, integrated microfluidics is mostly used in academia rather than in the many potential industrial applications. We have designed and manufactured a semiautomatic Microfluidic Device Assembly System (μDAS) for full device production. μDAS comprises an electrooptic mechanical system consisting of four main parts: optical system, smart media holder (for PDMS), a micropositioning xyzθ system and a macropositioning XY mechanism. The use of the μDAS yielded valuable information regarding PDMS as the material for device fabrication, revealed previously unidentified errors, and enabled optimization of a robust fabrication process. In addition, we have demonstrated the utilization of the μDAS technology for fabrication of a complex 3 layered device with over 12 000 micromechanical valves and an array of 64 × 64 DNA spots on a glass substrate with high yield and high accuracy. We increased fabrication yield from 25% to about 85% with an average layer alignment error of just ∼4 μm. It also increased our protein expression yields from 80% to over 90%, allowing us to investigate more proteins per experiment. The μDAS has great potential to become a valuable tool for both advancing integrated microfluidics in academia and producing and applying microfluidic devices in the industry.
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http://dx.doi.org/10.1039/c6lc01534dDOI Listing
January 2017
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