Publications by authors named "Yahia Cherrah"

68 Publications

Synthesis, α-glucosidase and α-amylase inhibitory activities, acute toxicity and molecular docking studies of thiazolidine-2,4-diones derivatives.

J Biomol Struct Dyn 2021 Apr 13:1-12. Epub 2021 Apr 13.

Laboratory of Pharmacology and Toxicology, Biopharmaceutical and Toxicological Analysis Research Team, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Rabat, Morocco.

In the present study, a series of thiazolidine-2,4-diones derivatives () and () were synthesized and characterized by H NMR, C NMR and ESI-MS spectrometry. All compounds were screened for their α-glucosidase and α-amylase inhibitory activities. biological investigations revealed that most of compounds were active against α-glucosidase with IC values in the range of 43.85 ± 1.06 to 380.10 ± 1.02 µM, and α-amylase with IC in the range of 18.19 ± 0.11 to 208.10 ± 1.80 µM. Some of the tested compounds were found to be more potent inhibitors than the clinical drug Acarbose (IC = 97.12 ± 0.35 µM and IC = 2.97 ± 0.004 μM). The lead compounds were evaluated for their acute toxicity on Swiss mice and found to be completely non-toxic with LD > 2000 mg/kg BW. Furthermore, the Structure-activity relationship (SAR) and the binding interactions of all compounds with the active site of α-glucosidase and α-amylase were confirmed through molecular docking and stabilizing energy calculations. This study has identified the inhibitory potential a new class of synthesized thiazolidine-2,4-diones in controlling both hyperglycemia and type 2 diabetes mellitus. Furthermore, the theoretical binding mode of the target molecules was evaluated by molecular docking studies against the 3D Crystal Structure of human pancreatic α-amylase (PDB ID: 1B2Y) and α-glucosidase (PDB ID: 3W37)Communicated by Ramaswamy H. Sarma.
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http://dx.doi.org/10.1080/07391102.2021.1911854DOI Listing
April 2021

Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection.

Molecules 2021 Feb 18;26(4). Epub 2021 Feb 18.

Medical Biotechnology Center, Moroccan Foundation for Advanced Science, Innovation & Research (MAScIR), Rabat 10100, Morocco.

Lateral flow assays (lateral flow immunoassays and nucleic acid lateral flow assays) have experienced a great boom in a wide variety of early diagnostic and screening applications. As opposed to conventional examinations (High Performance Liquid Chromatography, Polymerase Chain Reaction, Gas chromatography-Mass Spectrometry, etc.), they obtain the results of a sample's analysis within a short period. In resource-limited areas, these tests must be simple, reliable, and inexpensive. In this review, we outline the production process of antibodies against drugs of abuse (such as heroin, amphetamine, benzodiazepines, cannabis, etc.), used in lateral flow immunoassays as revelation or detection molecules, with a focus on the components, the principles, the formats, and the mechanisms of reaction of these assays. Further, we report the monoclonal antibody advantages over the polyclonal ones used against drugs of abuse. The perspective on aptamer use for lateral flow assay development was also discussed as a possible alternative to antibodies in view of improving the limit of detection, sensitivity, and specificity of lateral flow assays.
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http://dx.doi.org/10.3390/molecules26041058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922373PMC
February 2021

New insights into the Argan oil categories characterization: Chemical descriptors, FTIR fingerprints, and chemometric approaches.

Talanta 2021 Apr 6;225:122073. Epub 2021 Jan 6.

Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, CePhaR, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, B-1090, Brussels, Belgium. Electronic address:

The characterization of Argan oils to classify them in three categories ('Extra Virgin', 'Virgin' and 'Lower quality') was evaluated. A total of 120 Moroccan Argan oils samples from the Taroudant Argan forest was investigated. The free acidity, peroxide value, spectrophotometric indices (K232 and K270), fatty acids, sterols, and tocopherol contents were assessed. The samples were also scanned by FTIR spectroscopy. The Principal Component Analysis (PCA) and four classification methods, Partial Least Squares Discriminant Analysis (PLS-DA), Soft Independent Modelling of Class Analogy (SIMCA), K-nearest Neighbors (KNN), and Support Vector Machines (SVM), were applied on both the chemical and spectral data. Besides the conventional chemical profiling, FTIR spectra were evaluated for their feasibility as a rapid non-invasive approach for classifying and predicting the oil quality categories. The most important variables for differentiating the oil categories were identified as K, peroxide value, ɣ-tocopherol, δ-tocopherol, acidity, stigma-8-22-dien-3β-ol, stearic acid (C) and linoleic acid (C) and could be used as quality indicators. Eight chemical descriptors or key features from the FTIR spectra (selected by interval-PLS) could also be established as indicators of quality and freshness of Argan oils.
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http://dx.doi.org/10.1016/j.talanta.2020.122073DOI Listing
April 2021

Classification of polymorphic forms of fluconazole in pharmaceuticals by FT-IR and FT-NIR spectroscopy.

J Pharm Biomed Anal 2021 Mar 26;196:113922. Epub 2021 Jan 26.

Bio-Pharmaceutical and Toxicological Analysis Research Team, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat, Morocco.

The main goal of this work was to test the ability of vibrational spectroscopy techniques to differentiate between different polymorphic forms of fluconazole in pharmaceutical products. These are mostly manufactured with fluconazole as polymorphic form II and form III. These crystalline forms may undergo polymorphic transition during the manufacturing process or storage conditions. Therefore, it is important to have a method to monitor these changes to ensure the stability and efficacy of the drug. Each of FT-IR or FT-NIR spectra were associated to partial least squares-discriminant analysis (PLS-DA) for building classification models to distinguish between form II, form III and monohydrate form. The results has shown that combining either FT-IR or FT-NIR to PLS-DA has a high efficiency to classify various fluconazole polymorphs, with a high sensitivity and specificity. Finally, the selectivity of the PLS-DA models was tested by analyzing separately each of three following samples by FT-IR and FT-NIR: lactose monohydrate, which is an excipient mostly used for manufacturing fluconazole pharmaceutical products, itraconazole and miconazole. These two last compounds mimic potential contaminants and belong to the same class as fluconazole. Based on the plots of Hotelling's T² vs Q residuals, pure compounds of miconazole and itraconazole, that were analyzed separately, were significantly considered outliers and rejected. Furthermore, binary mixtures consist of fluconazole form-II and monohydrate form with different ratios were used to test the suitability of each technique FT-IR and FT-NIR with PLS-DA to detect minimum contaminant or polymorphic conversion from a polymorphic form to another using also the plots of Hotelling's T² vs Q residuals.
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http://dx.doi.org/10.1016/j.jpba.2021.113922DOI Listing
March 2021

Corrigendum to "Sedative and Hypnotic Activities of the Methanolic and Aqueous Extracts of from Morocco".

Adv Pharmacol Pharm Sci 2020 12;2020:5695093. Epub 2020 Nov 12.

Laboratory of Pharmacology and Toxicology, Department of Drugs Sciences, Faculty of Medicine and Pharmacy, Mohammed V Souissi University, ERTP, BP 6203, Rabat Instituts, Agdal, Rabat, Morocco.

[This corrects the article DOI: 10.1155/2012/270824.].
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http://dx.doi.org/10.1155/2020/5695093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701209PMC
November 2020

Evaluation of antidiabetic effect of Cistus salviifolius L. (Cistaceae) in streptozotocin-nicotinamide induced diabetic mice.

J Basic Clin Physiol Pharmacol 2020 Oct 5. Epub 2020 Oct 5.

Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Biopharmaceutical and Toxicological Analysis Research Team, Mohammed V University in Rabat, Rabat Institute, Rabat, Morocco.

Objectives Cistus salviifoluis L. is a shrub from Cistaceae family used in many traditional medicines for the treatment of various diseases including diabetes mellitus. The aim of this study was to evaluate the in vivo antidiabetic potential of the aerial parts aqueous extract of Cistus salviifolius L. (CSA). Methods Experimental diabetes was induced in adult male mice by intra-peritoneal injection of Streptozotocin-nicotinamide (STZ-NC). CSA at a dose of 500 mg/kg was administered daily to the diabetic mice for four weeks. The effect of the extract on hyperglycemia, body weight, serum total cholesterol, triglycerides, hepatic and renal functional markers were determined. Histopathological examination of the mice pancreas was also performed. The diabetic animals treated with CSA were compared with animals treated by the standard drug metformin. Results Treatment with CSA showed a significant reduction in blood glucose, total triglycerides and creatinine levels and prevented the reduction of body weight caused by diabetes. Furthermore, histopathological analysis of the mice pancreas showed that the group treated with CSA reduced damage induced by STZ-NC on islets of Langerhans cells when compared to the diabetic control. Conclusions The results suggest that the aqueous extract of Moroccan C. salviifolius L. possesses beneficial effect on treatment of diabetes.
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http://dx.doi.org/10.1515/jbcpp-2020-0044DOI Listing
October 2020

Comparative Study of Leaf and Rootstock Aqueous Extracts of on Chemical Profile and Antioxidant and Antihyperglycemic Activities.

Adv Pharmacol Pharm Sci 2020 1;2020:8852570. Epub 2020 Sep 1.

Biopharmaceutical and Toxicological Analysis Research Team, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco.

is a medicinal plant used in Moroccan folk medicine to treat several diseases such as diabetes. The aim of this study was to determine the phenolic bioactive compounds and to evaluate the antioxidant and antihyperglycemic activities of leaf and rootstock extracts. Phenolic compounds of rootstock and leaf extracts were determined using HPLC-DAD-QTOFMS analysis. The antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals. Moreover, the antihyperglycemic effects were tested by measuring the inhibition of -amylase and -glucosidase activities. HPLC-DAD-QTOFMS analysis identified thirty-two phenolic components in both leaf and rootstock extracts. Caffeic acid, quinic acid, and chlorogenic acid were the major compounds of leaf extract (FVLE), while the main compound of rootstock extracts (FVRE) was quinic acid. In the DPPH assay, leaf extract showed important antioxidant activity (IC = 12.16 ± 0.02 g/mL) than rootstock extract (IC = 34.36 ± 0.09 g/mL). Moreover, fennel leaf extracts revealed also the most powerful antioxidant activity (IC = 22.95 ± 0.4 g/mL) in the ABTS assay. The antihyperglycemic activity showed that rootstock extract exhibited a remarkable inhibitory capacity (IC = 194.30 ± 4.8 g/mL) of -amylase compared with leaf extract (IC = 1026.50 ± 6.5 g/mL). Furthermore, the inhibition of -glucosidase was more importantly with rootstock (IC of 165.90 ± 1.2 g/mL) than leaf extracts (203.80 ± 1.3 g/mL). The funding of this study showed that rootstock and leaf extracts presented several phenolic compounds and showed important antioxidant and antidiabetic effects. We suggest that the identified molecules are responsible for the obtained activities. However, further studies focusing on the isolation and the determination of antioxidant and antidiabetic effects of rootstock and leaf main compounds are required.
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http://dx.doi.org/10.1155/2020/8852570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481954PMC
September 2020

Platelets Can Associate with SARS-Cov-2 RNA and Are Hyperactivated in COVID-19.

Circ Res 2020 Sep 17. Epub 2020 Sep 17.

Infectious Diseases and Immunity, Centre de Recherche du CHU de Quebec, CANADA.

In addition to the overwhelming lung inflammation that prevails in COVID-19, hypercoagulation and thrombosis contribute to the lethality of subjects infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Platelets are chiefly implicated in thrombosis. Moreover, they can interact with viruses and are an important source of inflammatory mediators. While a lower platelet count is associated with severity and mortality, little is known about platelet function during COVID-19. To evaluate the contribution of platelets to inflammation and thrombosis in COVID-19 patients. Blood was collected from 115 consecutive COVID-19 patients presenting non-severe (n=71) and severe (n=44) respiratory symptoms. We document the presence of SARS-CoV-2 RNA associated with platelets of COVID-19 patients. Exhaustive assessment of cytokines in plasma and in platelets revealed the modulation of platelet-associated cytokine levels in both non-severe and severe COVID-19 patients, pointing to a direct contribution of platelets to the plasmatic cytokine load. Moreover, we demonstrate that platelets release their alpha- and dense-granule contents in both non-severe and severe forms of COVID-19. In comparison to concentrations measured in healthy volunteers, phosphatidylserine-exposing platelet extracellular vesicles were increased in non-severe, but not in severe cases of COVID-19. Levels of D-dimers, a marker of thrombosis, failed to correlate with any measured indicators of platelet activation. Functionally, platelets were hyperactivated in COVID-19 subjects presenting non-severe and severe symptoms, with aggregation occurring at suboptimal thrombin concentrations. Furthermore, platelets adhered more efficiently onto collagen-coated surfaces under flow conditions. Taken together, the data suggest that platelets are at the frontline of COVID-19 pathogenesis, as they release various sets of molecules through the different stages of the disease. Platelets may thus have the potential to contribute to the overwhelming thrombo-inflammation in COVID-19, and the inhibition of pathways related to platelet activation may improve the outcomes during COVID-19.
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http://dx.doi.org/10.1161/CIRCRESAHA.120.317703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641188PMC
September 2020

[Current antiplatelet agents, new inhibitors and therapeutic targets].

Med Sci (Paris) 2020 Apr 1;36(4):348-357. Epub 2020 May 1.

Research Center of Abulcasis University of Health Sciences, Rabat, Maroc - Faculty of Sciences, Department of Biology, Mohammed V University, Rabat, Maroc.

Cardiovascular diseases are the leading cause of deaths in the world. Platelets play a major role in the occurrence of these diseases and the development of antiplatelet drugs is a priority in the fight against cardiovascular diseases-associated mortality. Aspirin and thienopyridine-based P2Y12 inhibitors are the main drugs currently used. These molecules target the initiation of platelets activation and are responsible for a moderate inhibitory action. Other antiplatelet agents, as glycoprotein (GP) IIb/IIIa antagonists, inhibit platelet aggregation independently of initial activation-associated pathways, but are responsible for increased hemorrhagic events. Regarding each antiplatelet agent's specific characteristics, the prescription of these drugs must take into account the type of cardiovascular event, the age of the patient, the past medical history, and the potential hemorrhagic adverse events. Thus, there is a need for the development of new molecules with a more targeted effect, maintaining optimal efficiency but with a reduction of the hemorrhagic risk, which is the principal limitation of these treatments.
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http://dx.doi.org/10.1051/medsci/2020061DOI Listing
April 2020

Overview of recent developments of pyrazole derivatives as an anticancer agent in different cell line.

Bioorg Chem 2020 04 14;97:103470. Epub 2019 Dec 14.

Laboratory of Pharmacology and Toxicology, Bio Pharmaceutical and Toxicological Analysis Research Team, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, BP 6203, Rabat, Morocco.

Pyrazole is a five-membered aromatic heterocyclic ring with two adjacent nitrogen atoms CHNH.The presence of this nucleus in pharmacological agents of various therapeutic categories gifts a broad spectrum of biological activities and pharmaceuticals that contain pyrazole like celecoxib (anti-inflammatory), CDPPB (antipsychotic), Rimonabant (anti-obesity), Difenamizole, (Analgesic), Betazole (H2 receptor agonist), Fezolamide (Antidepressant), etc… The pharmacological potential of the pyrazole fraction is proved in many publication where they synthesized and evaluated pyrazoles against several biological agents. The aim of this article review is to survey recent works linking pyrazole structures to anticancer activities corresponding to 9 different type of cancer.
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http://dx.doi.org/10.1016/j.bioorg.2019.103470DOI Listing
April 2020

Therapeutic Characteristics, Chemotherapy-Related Toxicities and Survivorship in Colorectal Cancer Patients.

Ethiop J Health Sci 2020 Jan;30(1):65-74

Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy of Rabat, University Mohamed V Rabat, Avenue Mohammed Belarbi El Alaoui - Souissi - BP, 6203 Rabat, Morocco.

Background: Colorectal Cancer (CRC) is a major health problem around the globe. In Morocco, the disease ranks third after breast and lung cancers. This study is the first in Morocco to investigate epidemiological, clinical and therapeutic features while exhaustively describing toxic side-effects to chemotherapy of CRC and studying the 3-years survivorship.

Methods: This is a descriptive and analytical retrospective study of about 290 patients with CRC enrolled during the period of January-December 2013. Statistical analysis was performed to correlate clinicopathological data with chemotherapy toxicity and survivorship in patients, by Chi test. Overall Survival (OS) rate has been calculated by the Kaplan-Meier method and compared using Log-rank test.

Results: Fifty-five percent had a tumor localized in rectum, and 42,8% in colon. Mean age of these patients at diagnosis was 56,16 ± 14,6. incidence rate of adverse events (grade I to IV) was 85,6%. Diarrhea was the predominant toxicity (4.6%) occurring at a high grade (grade III-IV).The 3-years OS rate of patients with CRC was 71%. OS decreased by age, and patients with age subgroup between 40 to 59 years had a better OS than the other age subgroups (60 to 79 years and >80 years) with a p-value of 0.0001. Occurence of toxicity (all grades and types) was linked to a higher survival rates compared to the group who had no toxicity noticed (p-value of 0.001).

Conclusion: Our study shows that patients who had a polychemotherapy had a better OS than those who had monotherapy (p-value of 0.002).
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http://dx.doi.org/10.4314/ejhs.v30i1.9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036457PMC
January 2020

Discrimination of diesel fuels marketed in Morocco using FTIR, GC-MS analysis and chemometrics methods.

Talanta 2020 Mar 6;209:120543. Epub 2019 Nov 6.

Bio-Pharmaceutical and Toxicological Analysis Research Team, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat, Morocco. Electronic address:

The purpose of this study was to perform a discrimination and classification of diesel samples from the four major suppliers of petroleum products in Morocco using Fourier Transform Infrared Spectroscopy (FTIR), Gas Chromatography coupled with Mass Spectrometry (GC-MS) and chemometrics tools. Eighty diesel samples were collected from different gas stations owned by the four biggest brands in the Moroccan market. Principal Component Analysis (PCA) was performed to depict the similarities between the samples and check the presence of outliers. Partial Least Squares Discriminant Analysis (PLS-DA) models were set up for the discrimination and the classification of the four groups of samples (i.e., diesel suppliers). The models proposed in this study, were characterized by good prediction abilities, especially the FTIR-PLSDA model that was characterized by 100% of accurate discrimination of the four groups. The approach of analysis showed that the FTIR spectra can provide a cheap and rapid means for the determination of the diesel origin and to ensure the traceability of diesel products marketed in Morocco with respect for the rules of the green chemistry.
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http://dx.doi.org/10.1016/j.talanta.2019.120543DOI Listing
March 2020

Benzodiazepines use in Morocco: A nation wide consumption database study between 2004 and 2017.

Asian J Psychiatr 2020 Jan 10;47:101852. Epub 2019 Nov 10.

Centre de biotechnologie médicale, Moroccan foundation for Science, Innovation & Research (MAScIR), Rabat, Morocco. Electronic address:

Although information exists regarding the rate of benzodiazepines (BZDs) use in different countries, little information is available concerning the BZDs consumption in Morocco. To describe prescription rate in Morocco, a retrospective descriptive analysis of BZDs and their agonists use with the instituteIQIVIA database was performed during the period 2004-2017. The obtained data provide a dynamic approach to total BZDs consumption using an annual collection of sales data in Morocco and were expressed in terms of daily defined doses/ 1000 inhabitants / day. Data analysis showed that the major BZDs sold in Morocco were Alprazolam, Bromazepam, Nordazepam, Lorazepam, Parazepam, Diazepam and two benzodiazepine agonists, Zolpidem and Zopiclone. The Bromazepam was the molecule the most consumed during 2004-2016. In 2017, Alprazolam was the most consumed followed by Bromazepam, Nordazepam, Zolpidem, Lorazepam, Parazepam, Diazepam, Dipotassium clorazepate, Dipotassium Clorazypate and Zopiclone with 0.94, 0.91, 0.6, 0.55, 0.45, 0.32, 0.18, 0.18, 0.07 and 0.05 daily defined doses/ 1000 inhabitants / day respectively. The total amount consumed each year for all BZDs and their agonists in Morocco was 2.69, 2.77, 3, 3.17, 3.32, 3.54, 3.61, 3.81, 4.06, 4.30, 4.06, 3.94, 3.78 and 3.66 daily defined doses/ 1000 inhabitants / day, respectively during 2004-2017. To our knowledge, this is the first study that describes the consumption of BZDs and their agonists (Zolpidem, Zopiclone) in Morocco. This data may help the analytical toxicology laboratory and health organizations operating in the field of analytical biochemistry to develop specific BZDs quantification and detection methods needed for the Moroccan population.
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http://dx.doi.org/10.1016/j.ajp.2019.101852DOI Listing
January 2020

Diagnostic Utility of Human Leukocyte Antigen B*15:02 Screening in Severe Carbamazepine Hypersensitivity Syndrome.

Ann Indian Acad Neurol 2019 Oct-Dec;22(4):377-383. Epub 2019 Oct 25.

Laboratory of Pharmacology-Toxicology, Faculty of Medicine and Pharmacy, Rabat Institute University Mohamed V, Rabat, Morocco.

Background: Despite many studies suggesting an association between human leukocyte antigen (HLA)-B*15:02 and carbamazepine (CBZ)-induced severe cutaneous adverse drug reactions essentially toxic epidermal necrolysis (TEN) and Stevens-Johnson syndrome (SJS), the evidence of association in different populations and the degree of association remain uncertain.

Materials And Methods: The primary analysis was based on population control studies. Data were pooled by means of a random-effects model, and sensitivity, specificity, positive and negative likelihood ratios (LR+ and LR-), diagnostic odds ratios (DOR), and areas under the summary receiver operating characteristic curve (AUC) were calculated.

Results: In 23 population control studies, HLA-B*15:02 was measured in 373 patients with CBZ-induced TEN/SJS and 3452 patients without CBZ-induced TEN/SJS. The pooled sensitivity, specificity, LR+, LR-, DOR, and AUC were 0.67 (95% confidence interval [CI] = 0.63-0.72), 0.98 (95% CI = 0.98-0.99), 19.73 (95% CI = 10.54-36.92), 0.34 (95% CI = 0.23-0.49), 71.38 (95% CI = 34.89-146.05), and 0.96 (95% CI = 0.92-0.98), respectively. Subgroup analyses for Han Chinese, Thai, and Malaysian populations yielded similar findings. Specifically, racial/ethnic subgroup analyses revealed similar findings with respect to DOR for Han Chinese (99.28; 95% CI = 22.20-443.88), Thai (61.01; 95% CI = 23.05-161.44), and Malaysian (30; 95% CI = 7.08-126.68) populations, which are similar to the pooled DOR for the relationship between the HLA-B*15:02 allele and CBZ-induced TEN/SJS across all populations (71.38; 95% CI = 34.89-146.05).

Conclusions: The present study reveals that CBZ is the leading cause of TEN/SJS in many countries. Screening of HLA-B*15:02 may help patients to prevent the occurrence of CBZ-induced TEN/SJS, especially in populations with a higher (≥5%) risk allele frequency.
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http://dx.doi.org/10.4103/aian.AIAN_492_18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839291PMC
October 2019

The displacement study of Tc-DTPA-Human serum albumin binding in presence of furosemide and metformin by using equilibrium dialysis and molecular docking.

IUBMB Life 2019 12 21;71(12):2003-2009. Epub 2019 Oct 21.

Biopharmaceutical and Toxicological analysis research team, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat, Morocco.

The Tc-DTPA (Technetium diethylenetriaminepentaacetic acid), is a radiopharmaceutical used in renal scintigraphy. The human serum albumin (HSA) binding site(s) for the Tc-DTPA have never been characterized. This study will cover in vitro the binding rates of Tc-DTPA on HSA and the Tc-DTPA competition interactions with two drugs having known human serum albumin binding sites. Furosemide (FUR) and metformin (MET) were added to Tc-DTPA solution (weight ratios 1/1 vol:vol) followed by the quantification of Tc-DTPA binding rates to HSA (40 g/L) using equilibrium dialysis and the qualification of this binding using Molecular Modeling methods. The Tc-DTPA binding rates to human serum albumin increased with the highest concentration. Both drugs FUR and MET displaced Tc-DTPA binding. Tc-DTPA could bind to human serum albumin in many locations in site I and I-II, but strongly bound to site I through hydrogen bonds.
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http://dx.doi.org/10.1002/iub.2167DOI Listing
December 2019

The displacement study of Tc-DTPA-Human serum albumin binding in presence of furosemide and metformin by using equilibrium dialysis and molecular docking.

IUBMB Life 2019 12 21;71(12):2003-2009. Epub 2019 Oct 21.

Biopharmaceutical and Toxicological analysis research team, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat, Morocco.

The Tc-DTPA (Technetium diethylenetriaminepentaacetic acid), is a radiopharmaceutical used in renal scintigraphy. The human serum albumin (HSA) binding site(s) for the Tc-DTPA have never been characterized. This study will cover in vitro the binding rates of Tc-DTPA on HSA and the Tc-DTPA competition interactions with two drugs having known human serum albumin binding sites. Furosemide (FUR) and metformin (MET) were added to Tc-DTPA solution (weight ratios 1/1 vol:vol) followed by the quantification of Tc-DTPA binding rates to HSA (40 g/L) using equilibrium dialysis and the qualification of this binding using Molecular Modeling methods. The Tc-DTPA binding rates to human serum albumin increased with the highest concentration. Both drugs FUR and MET displaced Tc-DTPA binding. Tc-DTPA could bind to human serum albumin in many locations in site I and I-II, but strongly bound to site I through hydrogen bonds.
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http://dx.doi.org/10.1002/iub.2167DOI Listing
December 2019

In vivo anti-inflammatory response and bioactive compounds' profile of polyphenolic extracts from edible Argan oil (Argania spinosa L.), obtained by two extraction methods.

J Food Biochem 2019 12 1;43(12):e13066. Epub 2019 Oct 1.

Pharmacodynamy Research Team ERP, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco.

The present work examined and assessed the in vivo anti-inflammatory effect of polyphenolic extracts from Moroccan edible Argan oils (Argania spinosa L.), extracted by two extraction processes: Hand pressing and mechanical pressing. Chemical properties, such as acidity, peroxide index, ultraviolet indices, total polyphenols composition, fatty acid composition, tocopherol composition, phenolic profiling, and sterol composition were studied. Then, the anti-inflammatory potential was determined by applying carrageenan, an induced paw edema test in rats. The results revealed an anti-inflammatory effect of edible Argan oil and indicated a higher efficiency of hand-pressed oil compared to mechanical-pressed oil, supporting its traditional use in human health, related to pain and inflammations. The chemical composition of these oils was evaluated, and total polyphenols, tocopherol composition, and some phenolic compounds were found highly concentrated in the hand-pressed oil. PRACTICAL APPLICATIONS: The present study highlights and compares the in vivo anti-inflammatory effect of polyphenolic compounds, extracted from Argan oil by two processes (hand and mechanical extraction). The study demonstrated the better quality of hand-pressed oil over mechanically pressed, supporting the traditional uses of this oil in treating several inflammations and pain-related situations. Moreover, the edible Argan oil may be introduced as a regular diet and food ingredient.
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http://dx.doi.org/10.1111/jfbc.13066DOI Listing
December 2019

Impact of Disparities in Reimbursement Rules Between Public and Private Sectors on Accessibility to Care in Moroccan Mandatory Health Insurance: A Cross-Sectional Study.

Value Health Reg Issues 2019 Sep 27;19:132-137. Epub 2019 Aug 27.

Mohammed V University, Faculty of Medicine and Pharmacy, Cheikh Zaid Hospital, Rabat, Morocco.

Background: Disparities in the reimbursement rules between the 2 funds that manage mandatory health insurance in Morocco could negatively affect the accessibility of insured persons to healthcare services and products.

Objective: The objective is to analyze the impact of these disparities on access to care and to assess the insured's copayment difference between the 2 funds.

Methods: Healthcare utilization rates of the insured population in the 2 funds were analyzed by sector, sex, and age groups for 2014. We also looked at the percentage of copayment paid by the insured depending on the fund, methods of reimbursement, type of care, and nature of diseases. The analysis was based on data retrieved and aggregated at the National Agency for Health Insurance.

Results: The healthcare utilization rate differs significantly between the 2 funds. It is higher for the insured in the public sector (45%) compared with those in the private sector (18.5%) (P < .001). The healthcare utilization rate differs significantly according to the age groups in the 2 sectors (P < .001, respectively), and according to the sex of the insured in the 2 sectors (the healthcare utilization rate is higher for women than for men [P < .001, respectively]). The copayment percentage incurred by insured persons was 32.1% for employees in the public sector and 36.4% for employees in the private sector.

Conclusion: Differences in reimbursement rules between the 2 funds may be the cause of inequity in access to care between insured persons. This situation can jeopardize the objectives of a universal and equitable health insurance scheme.
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http://dx.doi.org/10.1016/j.vhri.2019.07.008DOI Listing
September 2019

Synthesis of Surfactants Derived from 2-Mercaptobenzimidazole and Study of Their Acute Toxicity and Analgesic and Psychotropic Activities.

Biochem Res Int 2019 30;2019:9615728. Epub 2019 Jul 30.

Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University, BP 6203 Rabat-Institutes, Rabat, Morocco.

The aim of the present study is to synthesize cationic salts from a relatively toxic compound named 2-mercaptobenzimidazole and to evaluate some of their pharmacological properties. The acute toxicity of these salts is evaluated according to OECD 423 Guidelines at the doses of 300 and 2000 mg/kg; their peripheral analgesic effect is studied using the Koster test at the therapeutic dose of 200 mg/kg and their sedative action is evaluated using Traction, Chimney, Hole-board, and Rotarod tests at the doses of 200 and 400 mg/kg. All synthesized molecules show no acute toxicity according to OECD Code 423 guidelines at doses ranging from 300 to 2000 mg/kg and do not cause any obesity or anorexia. Also, the results of the Koster test show that the studied compounds have an average analgesic effect at the dose of 200 mg/kg compared to acetylsalicylic acid. In addition, the elaborated compounds have shown a moderate sedative effect at the dose of 400 mg/kg, in comparison to 2-mercaptobenzimidazole (400 mg/kg) and Bromazepam (20 mg/kg). These compounds have no cataleptic and hypnotic effects on the central nervous system at the doses of 200 and 400 mg/kg. These results argue in favor of a possible integration of the most active salts tested in the pharmaceutical industry owing to their analgesic and sedative effects.
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http://dx.doi.org/10.1155/2019/9615728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6701276PMC
July 2019

[Traditional camphor misuse: a forgotten danger to children (about 2 cases)].

Pan Afr Med J 2019 26;32:89. Epub 2019 Feb 26.

Laboratoire de Pharmacologie et de Toxicologie, Faculté de Médecine et de Pharmacie, Université Mohammed V, Rabat, Maroc.

In our country, the use of traditional medicinal recipes and artisan cosmetic products is very frequent due to the high rate of illiteracy, low purchasing power and of the large number of herbalists. Camphor is a low-cost product, easily accessible and omnipresent in almost every home, making it potentially toxic in case of misuse, in particular among children. We here report two cases of intoxication induced by beauty recipe made with powdered camphor. Patients' medical history gave informations about poisoning in 2 children caused by synthetic powder made with camphor imported from China. Patient 1: little girl aged 2 months, with no previous medical history, admitted to the Emergency Department due to constant crying and refusal to eat. Clinical examination showed no abnormalities. Standard laboratory tests were normal. Neurological, digestive and cutaneous monitoring were performed. Patient 2: girl aged 6 years admitted with atonic seizure associated with syncope and foaming followed by abdominal pain and vomiting after ingesting milk. The evolution was favorable 48 hours after symptom management. Mothers reported two neighbors had received a traditional hair care recipe by a third neighbor. After that they had mixed powdered camphor with olive oil, then they had applied it to the hair of their children for one hour, thus causing the occurrence of these symptoms.
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http://dx.doi.org/10.11604/pamj.2019.32.89.17943DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560990PMC
July 2019

Incidence of adverse reactions caused by first-line anti-tuberculosis drugs and treatment outcome of pulmonary tuberculosis patients in Morocco.

Infection 2020 Feb 4;48(1):43-50. Epub 2019 Jun 4.

Equipe de Recherche de Pharmacoéconomie et Pharmacoépidémiologie, Laboratoire de Pharmacologie et Toxicologie, Faculté de Médecine et de Pharmacie, Université Mohammed V, Rabat, Morocco.

Purpose: The treatment of tuberculosis is associated with a high incidence of adverse reactions with different degrees of severity. The aim of this study was to determine the incidence of adverse reactions caused by first-line anti-tuberculosis drugs and to evaluate the treatment outcome of TB patients in a large region of Morocco.

Methods: It is a multi-centric observational cohort study conducted from January 01, 2014 to January 01, 2016. A questionnaire was established for data collection from clinical charts of TB patients. The study was carried out in all the 18 centers located in the Rabat-Salé-Kénitra region of Morocco where tuberculosis is treated. Adverse reactions are evaluated from the start of TB treatment until its end by a specialist clinician. The treatment outcomes are evaluated, and the definitions and classifications of these outcomes are defined according to World Health Organization guidelines.

Results: Among a total number of 2532 patients treated for TB, the average age is 37.3 ± 16.4 years, 10.0% of patients produced adverse reactions. 7.4% of adverse reactions are gastrointestinal, 3.7% are cutaneous, 2.0% are hepatic, 1.14% are articular, 1.07% are immunoallergic, 0.7% are neuropsychiatric, and 0.1% are ocular. The treatment outcome of TB patients is 79.1% rate for successful treatment and 15.6% for unsuccessful treatment.

Conclusion: Adverse reactions caused by anti-TB drugs are frequent among patients with TB. These ADRs must be followed up by a closer monitoring during anti-TB treatment period. Treatment success outcome in our study is slightly lower than the success rate target of WHO of at least 85%.
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http://dx.doi.org/10.1007/s15010-019-01324-3DOI Listing
February 2020

Contribution of fibrin glue in the surgery of cyanogenic and non-cyanogenic congenital cardiopathies: retrospective cohort study.

BMC Cardiovasc Disord 2019 05 16;19(1):117. Epub 2019 May 16.

Department of Pediatric Cardiac Surgery, Cheikh Zaid Hospital, Rabat, Morocco.

Background: Postoperative bleeding in cardiovascular surgery is a frequent and complicated situation for the surgical team, and may also be responsible for significant hospital expenditures. Fibrin glue are indicated in surgery to improve hemostasis when conventional techniques such as compression, sutures or electrocoagulation are insufficient. Through this study, we tried to study the contribution of fibrin glue to the improvement of the clinical parameters (volume of postoperative bleeding, length of stay in intensive care, volume of blood transfusion ...) in two populations having undergone cardiac surgery, one in which we used the fibrin glue and one without fibrin glue.

Methods: This was a retrospective cohort study conducted in the cardiovascular surgery department of our Hospital in Rabat between June 2012 and June 2015. Fibrin glue (Tissucol® of BAXTER) was used in one group with an haemostatic aim. The pre and post-operative clinical data of the patients were analyzed and compared with data from patients who were operated without the use of fibrin glue because it was not yet available in the hospital. The clinical parameters were collected analyzed using the SPSS 13.0 software.

Results: One hundred ten patients were included in this study. The fibrin glue was used intraoperatively in 55 patients and not used in 55 patients. 43 (39.1%) had cyanogenic diseases and 67 (60.9%) had non-cyanogenic pathologies. The volume of transfused red blood cells was lower in patients in whom we used biological glue (p = 0.005), as well as the number of days spent in intensive care (p = 0.02). However, the difference was not significant between the two groups for other parameters such as bleeding volume per kg, the number of units of fresh frozen plasma and the platelet units count transfused.

Conclusions: The results we found show that fibrin glue reduces the duration of hospitalization in resuscitation and reduces the number of units of transfused red blood cells to patients after surgery. However, it does not reduce significantly the total postoperative bleeding volume per weight, the number of fresh frozen plasma units or platelets units transfused. The fibrin glue could therefore be of moderate benefit in pediatric cardiac surgery.
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http://dx.doi.org/10.1186/s12872-019-1102-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6524237PMC
May 2019

Treatment outcomes of drug resistant tuberculosis patients in Morocco: multi-centric prospective study.

BMC Infect Dis 2019 Apr 11;19(1):316. Epub 2019 Apr 11.

Equipe de Recherche de Pharmacoéconomie & Pharmacoépidémiologie. Laboratoire de Pharmacologie & Toxicologie, Faculté de Médecine et de Pharmacie, Université Mohammed V, Rabat, Morocco.

Background: Drug resistant tuberculosis is a major public health problem in Morocco and worldwide. Treatment outcome of drug resistant tuberculosis is poor and requires a long period of treatment with many toxic and expensive antituberculosis drugs. The aim of this study is to evaluate treatment outcomes of drug resistant tuberculosis and to determine predictors of poor treatment outcomes in a large region of Morocco.

Methods: It is a multi-centric observational cohort study conducted from January 01, 2014 to January 01, 2016. A questionnaire was established to collect data from clinical charts of patients with confirmed resistant TB. The study was carried out in all the 11 centers located in the Rabat-Salé-Kénitra region of Morocco where drug resistant tuberculosis is treated. Treatment outcomes were reported and the definitions and classifications of these outcomes were defined according to the WHO guidelines. Univariate and multivariate logistic regression were conducted to determine factors associated with poor drug resistant tuberculosis treatment outcomes in Morocco.

Results: In our study, 101 patients were treated for drug resistant tuberculosis between January 01, 2014 and January 01, 2016. Patients' age ranged from 9.5 to70 years; 72patients (71.3%) were male and 80 patients (79.2%) were living in urban areas. Thirty two patients were smokers, 74 patients had multidrug-resistant tuberculosis, 25 patients had rifampicin resistance and 2 patients had isoniazid resistance. Treatment outcomes of tuberculosis patients were as follows: 45 patients were cured (44.5%), 9 completed treatment (8.9%), 5 patients died before completing the treatment, 35 patients were lost to follow up (34.6%) and 7 patients had treatment failure. In the multivariate analysis, being a smoker is an independent risk factor for poor treatment outcomes, (p-value = 0.015, OR = 4.355, IC [1.327-14.292]).

Conclusion: Treatment success outcomes occurred in more than half of the cases, which is lower than the World Health Organization target of at least a 75% success rate. A significant number of patients abandoned their treatment before its completion. These dropouts are a serious public health hazard that needs to be addressed urgently.
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http://dx.doi.org/10.1186/s12879-019-3931-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458640PMC
April 2019

Arbutus unedo L., (Ericaceae) inhibits intestinal glucose absorption and improves glucose tolerance in rodents.

J Ethnopharmacol 2019 May 8;235:385-391. Epub 2019 Feb 8.

Laboratoires TBC, Faculty of Pharmaceutical and Biological Sciences, Lille, France. Electronic address:

Ethnopharmacological Relevance: Arbutus unedo L., (Ericaceae) is one of the most traditional plants commonly used to treat diabetes in people living in Eastern Morocco region particularly in Taza and Beni Mellal.

Aim Of The Study: The aim of the study was to find if there is a scientific support to the ethnopharmacological relevance use of Arbutus unedo L., roots bark (AU) to treat diabetes.

Materials And Methods: We studied the effects of crude aqueous extract of AU on intestinal glucose absorption using short-circuit current technique in vitro and oral glucose tolerance test in vivo.

Results: The aqueous extract of AU (10 µg/mL to 1 mg/mL) induced concentration-dependent inhibition of sodium-dependent glucose transport across isolated mouse jejunum. The maximal inhibition was obtained with 1 mg/mL, which exhibited more than 80% of the Phloridzin inhibition with an IC close to 216 µg/mL. A 6-week AU ingestion (2 g/(kg day)), improved oral glucose tolerance as efficiently as metformin (300 mg/(kg day)). Arbutus unedo L. and metformin also reduced body weight.

Conclusions: Arbutus unedo L. roots bark aqueous extract directly inhibited the electrogenic intestinal absorption of glucose in vitro. In addition it improved oral glucose tolerance and lowered body weight in rats after chronic oral administration in vivo. These results add a scientific support to the ethnopharmacological relevance use of Arbutus unedo L. roots bark to treat diabetes.
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http://dx.doi.org/10.1016/j.jep.2019.02.013DOI Listing
May 2019

Integrative herbal treatments of diabetes in Beni Mellal region of Morocco.

J Integr Med 2019 Mar 5;17(2):93-99. Epub 2019 Jan 5.

Faculty of Medicine and Pharmacy, Pharmacokinetic Team, Laboratory of Pharmacology and Toxicology, Université Mohammed V-Souissi, Rabat 10056, Morocco.

Objectives: Diabetes is one of the most life-threatening chronic metabolic disorders and is considered a global health problem due to its prevalence and incidence. In Morocco, several herbal preparations are utilized to treat this disease. For this reason, the current study aimed to identify and to collect data about the medicinal plants utilized in folk medicine for the treatment of diabetes in the Beni Mellal region of Morocco.

Methods: An ethnobotanical survey was carried out among 400 herbalists, competent villagers and traditional healers from the Beni Mellal region through direct interviews using a semistructured questionnaire.

Results: Forty-five medicinal plants belonging to 25 families were identified for their use in diabetes treatment. Interview results showed that the most frequently used plants were Olea europaea, Salvia officinalis, Allium sativum and Trigonella foenum-graecum, with a relative frequency of citation values of 24.3%, 23.0%, 22.5% and 20.5%, respectively. Moreover, in this study, the Fabaceae family was the most commonly reported plant family, and the leaves and roots were the most commonly used parts, for the treatment of diabetes.

Conclusion: The Beni Mellal region of Morocco has an important floristic biodiversity of plants used to treat diabetes in traditional medicinal practice. This result provides a good database for pharmacological screenings in the search for plant-based antidiabetic medications.
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http://dx.doi.org/10.1016/j.joim.2019.01.001DOI Listing
March 2019

Discrimination and Quantification of Moroccan Gasoline Adulteration with Diesel Using Fourier Transform Infrared Spectroscopy and Chemometric Tools.

J AOAC Int 2019 May 23;102(3):966-970. Epub 2018 Oct 23.

Vrije Universiteit Brussel (VUB), Center for Pharmaceutical Research, Department of Analytical Chemistry and Pharmaceutical Technology, Laarbeeklaan 103, B-1090 Brussels, Belgium.

In this work, transform-infrared spectroscopy (FTIR) was associated with chemometric tools, especially principal component analysis (PCA) and partial least squares regression (PLSR), to discriminate and quantify gasoline adulteration with diesel. The method is composed of a total of 100 mixtures were prepared, and then FTIR fingerprints were recorded for all samples. PCA was used to verify that mixtures can be distinguished from pure products and to check that there are no outliers. As a result of using just PC1 and PC2, more than 98% of the general variability was explained. The PLSR model based on infrared spectra has shown its capabilities to be suitable for predicting gasoline adulteration in the concentration range of 0 to 98% (w/w), with a high significant coefficient of determination (² = 99.25%) and an acceptable calibration and prediction errors (root mean squared error of calibration = 0.63 and root mean square of external validation and/or prediction = 0.69).
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http://dx.doi.org/10.5740/jaoacint.18-0179DOI Listing
May 2019

Detection and analysis of drug-drug interactions among hospitalized cardiac patients in the Mohammed V Military Teaching Hospital in Morocco.

Pan Afr Med J 2018 24;29:225. Epub 2018 Apr 24.

Laboratory of Pharmacology, Toxicology, Faculty of Medicine and Pharmacy, Rabat Institute, University Mohamed V, Rabat-Morocco.

Introduction: Drug-drug interactions (DDIs) are defined as two or more drugs interacting in such a manner that the effectiveness or toxicity of one or more drugs is altered. Patients with cardiovascular disorders are at higher risk for DDIs because of the types and number of drugs they receive. The aim of the present study was to assess the prevalence of DDIs in patients admitted to the cardiology department of a hospital in Morocco.

Methods: A prospective observational study from June 2016 to September 2016 was carried out in the cardiology department of a hospital in Morocco. Those patients who were taking at least two drugs and had a hospital stay of at least 48 hours were included in the study. The medications of the patients were analysed for possible interactions. All the prescriptions of the study population were screened for drug-drug interactions using a computerized DDI database system (Theriaque).

Results: During the study period, 138 patients were included; 360 interactions were detected among 94 patients, with an average number of drugs taken of 5.2. The prevalence of DDIs was estimated at 68.11%, the most common of which concerned Kardegic/Plavix (12.22%), Kardegic/Heparin (8.33%), and Lasilix/Spironolactone (5.83%). Among the 726 prescribed drugs, (372 [51.24%]) were drugs of the cardiovascular system, followed by blood and hematopoietic organ drugs (288 [39.67%]) according to the Anatomical Therapeutic Chemical Classification codes. These interactions were categorized on the basis of level of severity: interactions with major severity accounted for 11.11% (40) of the total DDIs while those with moderate and minor severity accounted for 37.22% (134) and 51.66% (186), respectively.

Conclusion: This study reports the prevalence of DDIs in patients admitted to the cardiology department of a hospital in Morocco. This study shows that DDIs are frequent among hospitalized cardiac patients and highlights the need to screen prescriptions of cardiovascular patients for possible DDIs, as this helps in their detection and prevention.Pan African Medical Journal - ISSN: 1937- 8688 (www.panafrican-med-journal.com)Published in partnership with the African Field Epidemiology Network (AFENET). (www.afenet.net)Pan African Medical Journal - ISSN: 1937- 8688 (www.panafrican-med-journal.com)Published in partnership with the African Field Epidemiology Network (AFENET). (www.afenet.net).
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http://dx.doi.org/10.11604/pamj.2018.29.225.14169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080962PMC
August 2018

Synthesis and Pharmacological Valorization of Derivatives of 4-Phenyl-1,5-Benzodiazepin-2-One.

Adv Pharmacol Sci 2018 1;2018:6042602. Epub 2018 Apr 1.

PharmacodynamyResearch Team PRT, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University, BP 6203, Rabat, Morocco.

The objective of our work is to make a pharmacological study of molecules derived from 4-phenyl-1,5-benzodiazepin-2-one carrying long chains so that they have a structure similar to surfactants, with the benzodiazepine as a hydrophilic head and a carbon chain as a hydrophobic tail. First, we studied the acute toxicity of the above mentioned 4-phenyl-1,5-benzodiazepin-2-one derivatives. This study was conducted according to OECD 423 guidelines in female mice and revealed that these compounds are nontoxic. We then assessed the psychotropic effects of our products on the central nervous system (CNS). The results obtained show that 4-phenyl-1,5-benzodiazepin-2-one has no sedative effect at therapeutic doses of 100 and 200 mg/kg. On the other hand, its long-chain derivatives possess them. Moreover, all these products have no cataleptic and hypnotic effects at the doses studied. But at 100 mg/kg, these compounds all have the ability to significantly prolong the hypnotic effect of thiopental sodium.
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http://dx.doi.org/10.1155/2018/6042602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902104PMC
April 2018

Selected-ion flow-tube mass-spectrometry (SIFT-MS) fingerprinting versus chemical profiling for geographic traceability of Moroccan Argan oils.

Food Chem 2018 Oct 18;263:8-17. Epub 2018 Apr 18.

Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, CePhaR, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, B-1090 Brussels, Belgium. Electronic address:

This study investigated the effectiveness of SIFT-MS versus chemical profiling, both coupled to multivariate data analysis, to classify 95 Extra Virgin Argan Oils (EVAO), originating from five Moroccan Argan forest locations. The full scan option of SIFT-MS, is suitable to indicate the geographic origin of EVAO based on the fingerprints obtained using the three chemical ionization precursors (HO, NO and O). The chemical profiling (including acidity, peroxide value, spectrophotometric indices, fatty acids, tocopherols- and sterols composition) was also used for classification. Partial least squares discriminant analysis (PLS-DA), soft independent modeling of class analogy (SIMCA), K-nearest neighbors (KNN), and support vector machines (SVM), were compared. The SIFT-MS data were therefore fed to variable-selection methods to find potential biomarkers for classification. The classification models based either on chemical profiling or SIFT-MS data were able to classify the samples with high accuracy. SIFT-MS was found to be advantageous for rapid geographic classification.
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http://dx.doi.org/10.1016/j.foodchem.2018.04.059DOI Listing
October 2018

[Which place occupies the free drug sample in the prescription of the physicians in Morocco?]

Therapie 2018 Oct 13;73(5):377-383. Epub 2018 Apr 13.

Équipe de recherche pharmacoéconomie et pharmacoépidémiologie, laboratoire de pharmacologie et toxicologie, faculté de médecine et de pharmacie, université Mohammed V de Rabat, 6203 Rabat, Maroc. Electronic address:

Objectives: Do the state of affairs of the perception of free drug samples by a group of prescribers of drugs in the Morocco.

Methods: This was a cross-sectional study of 381 physicians practising in different sectors (public, private) using a self-administered anonymous questionnaire, conducted between December 2016 and March 2017. The questionnaire focused on assessing the general knowledge of prescriber on free drug sample, medical visit of medical representative of pharmaceutical laboratory, drug prescribing and free drug sample use.

Results: In total, 381 prescribers were interviewed. Sex M/F ratio was 0.53 with a mean age of 37.4±11.2 years exerting mainly in the public sector in urban areas. According to surveyed prescribers, the drug specialties presented as free drug sample occupied 52.1 percent of their prescription. The main interest in having the free drug sample was to help the patient at low socioeconomic level but it influences the physician's prescription.

Conclusion: This survey puts in perspective the place of the free drug sample in the medical prescription with prescribers, in order to raise awareness about the ability of free drug sample to influence the medical prescription although it allows to help the patient low socio-economic level.
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http://dx.doi.org/10.1016/j.therap.2018.04.001DOI Listing
October 2018