Publications by authors named "Yadong Tang"

17 Publications

  • Page 1 of 1

Fabrication and in vitro evaluation of PCL/gelatin hierarchical scaffolds based on melt electrospinning writing and solution electrospinning for bone regeneration.

Mater Sci Eng C Mater Biol Appl 2021 Sep 1;128:112287. Epub 2021 Jul 1.

School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China. Electronic address:

As an emerging 3D printing technique, melt electrospinning writing (MEW) has been used to fabricate scaffolds with controllable structure and good mechanical strength for bone regeneration. However, how to further improve MEW scaffolds with nanoscale extracellular matrix (ECM) mimic structure and bioactivity is still challenging. In this study, we proposed a simple composite process by combining MEW and solution electrospinning (SE) to fabricate a micro/nano hierarchical scaffold for bone tissue engineering. The morphological results confirmed the hierarchical structure with both well-defined MEW microfibrous grid structure and SE random nanofiber morphology. The addition of gelatin nanofibers turned the scaffolds to be hydrophilic, and led to a slight enhancement of mechanical strength. Compared with PCL MEW scaffolds, higher cell adhesion efficiency, improved cell proliferation and higher osteoinductive ability were achieved for the MEW/SE composite scaffolds. Finally, multilayer composite scaffolds were fabricated by alternately stacking of MEW layer and SE layer and used to assess the effect on cell ingrowth in the scaffolds. The results showed that gelatin nanofibers did not inhibit cell penetration, but promoted the three-dimensional growth of bone cells. Thus, the strategy of the combined use of MEW and SE is a potential method to fabricate micro/nano hierarchical scaffolds to improve bone regeneration.
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http://dx.doi.org/10.1016/j.msec.2021.112287DOI Listing
September 2021

Kinetics of Mn(II) adsorption and catalytic oxidation on the surface of ferrihydrite.

Sci Total Environ 2021 Oct 4;791:148225. Epub 2021 Jun 4.

Key Laboratory of Poyang Lake Basin Agricultural Resource and Ecology of Jiangxi Province, College of Land Resource and Environment, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address:

Mn(II) adsorption-oxidation on iron (Fe) oxides (e.g., ferrihydrite) occurs in various soils and sediments, significantly affecting the toxicities and bioavailabilities of Mn and other associated elements. However, the detailed processes of Mn(II) adsorption-oxidation on ferrihydrite remain elusive. In this study, the Mn(II) (2 mM) adsorption-oxidation kinetics on different masses of ferrihydrite (0.25, 0.50, 1.00, and 1.25 g) at pH 7 were determined using batch kinetic studies combined with X-ray diffraction, transmission electron microscopy, and wet chemistry analyses. The results indicated that the low-concentration Mn(II) adsorption-oxidation on ferrihydrite occurred in two steps. First, Mn(II) was adsorbed onto ferrihydrite, where it was partially oxidized by the catalytic effect of ferrihydrite, within ~0-60 min; subsequently, the remaining Mn(II) underwent autocatalytic oxidation on the previously generated Mn (oxyhydr)oxides. The initial adsorption-oxidation behaviors of Mn(II) on the ferrihydrite surface determined the kinetics of Mn(II) removal and oxidation, and therefore the amounts and types of Mn (oxyhydr)oxides formed. Furthermore, the specific characteristics of Mn(II) adsorption-oxidation on ferrihydrite showed a strong dependence on the Fe/Mn molar ratio. When this ratio was below 16.35, the initial process was dominated by Mn(II) adsorption onto ferrihydrite, with slight oxidation generating hausmannite (~0-60 min), followed by the catalytic oxidation of Mn(II) on the formed hausmannite, generating manganite or groutite. Conversely, when the Fe/Mn molar ratio was above 32.7, the reactions primarily involved Mn(II) adsorption onto ferrihydrite with minor oxidation to form Mn(III/IV) (oxyhydr)oxides (~0-60 min), followed by the autocatalytic oxidation of Mn(II) on the freshly-generated Mn(III/IV) (oxyhydr)oxides, forming Mn(III) (oxyhydr)oxides, i.e., feitknechtite. These results provide further insight into the interaction between Fe and Mn, Mn(II) removal, and Mn (oxyhydr)oxide formation in the environment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148225DOI Listing
October 2021

Coaxial electrospun PVA/PCL nanofibers with dual release of tea polyphenols and ε-poly (L-lysine) as antioxidant and antibacterial wound dressing materials.

Int J Pharm 2021 May 27;601:120525. Epub 2021 Mar 27.

School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China; School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, China. Electronic address:

Preparing wound dressing with dual-delivery of antioxidant and antibacterial agents is highly desirable in clinical wound treatment. Herein, a series of coaxial nanofiber membranes loaded with antioxidant tea polyphenols (TP) in the core and antibacterial ε-poly (L-lysine) (ε-PL) in the shell layer were successfully fabricated by coaxial electrospinning. The physicochemical characterizations by transmission electron microscopy, inverted fluorescence microscopy and fourier transform infrared spectroscopy confirmed the formation of core-shell structure. The results of in vitro drug release indicated that ε-PL exhibited a fast release profile while TP released in a sustained manner, which is favorable to the achievement of quick bacteria inhibition in the initial phase as well as long-term antioxidant activity during wound healing. The antioxidant activity of coaxial nanofibers was found to be increased with the increment of TP content and incubation time. The antibacterial assays against Escherichia coli and Staphylococcus aureus demonstrated that the incorporation of ε-PL in the coaxial nanofibers led to strong antibacterial activity. Additionally, all the coaxial nanofibers possessed good cytocompatibility. Therefore, the prepared coaxial nanofibers simultaneously incorporated with ε-PL and TP are promising as potential wound dressing materials.
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http://dx.doi.org/10.1016/j.ijpharm.2021.120525DOI Listing
May 2021

Design, synthesis, and biological activity of novel semicarbazones as potent Ryanodine receptor1 inhibitors of Alzheimer's disease.

Bioorg Med Chem 2021 01 26;29:115891. Epub 2020 Nov 26.

Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, 103 Wenhua Road, Shenhe District, Shenyang 110016, PR China; Department of Pharmacology, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang 110016, PR China. Electronic address:

Ryanodine receptors (RyRs) are important ligand-gated Ca channels; their excessive activation leads to Ca leakage in the sarcoplasmic reticulum that may cause neurological diseases. In this study, three series of novel potent RyR1 inhibitors based on dantrolene and bearing semicarbazone and imidazolyl moieties were designed and synthesized, and their biological activity was evaluated. Using a single-cell calcium imaging method, the calcium overload inhibitory activities of 26 target compounds were tested in the R614C cell line, using dantrolene as a positive control. The preliminary investigation showed that compound 12a suppressed Ca release as evidenced by store overload-induced Carelease (SOICR) (31.5 ± 0.1%, 77.2 ± 0.1%, 93.7 ± 0.2%) at 0.1 μM, 3 μM and 10 μM, respectively. Docking simulation results showed that compound 12a could bind at the active site of the RyR1 protein. The Morris water-maze test showed that compound 12a significantly improved the cognitive behavior of AD-model mice. Further studies on the structural optimization of this series of derivatives are currently underway in our laboratory.
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http://dx.doi.org/10.1016/j.bmc.2020.115891DOI Listing
January 2021

Multidrug-loaded electrospun micro/nanofibrous membranes: Fabrication strategies, release behaviors and applications in regenerative medicine.

J Control Release 2021 02 21;330:1264-1287. Epub 2020 Nov 21.

School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China; School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, China. Electronic address:

Electrospun micro/nanofibrous membranes (EFMs) have been widely investigated as local drug delivery systems. Multiple drugs can be simultaneously incorporated into one EFM to create synergistic effects, reduce side effects, and play their respective roles in the complex physiological processes of tissue regeneration and postoperative adhesion prevention. Due to the versatile electrospinning techniques, sustained and programmed release behaviors of multiple drugs could be achieved by modulating the structure of the EFMs and the location of the drugs. In this review, various multidrug incorporation approaches based on electrospinning are overviewed. In particular, the advantages and limitations of each drug incorporation technique, the methods to control drug release and the effect of one drug release on another are discussed. Then the applications of multidrug-loaded EFMs in regenerative medicine, including wound healing, bone regeneration, vascular tissue engineering, nerve regeneration, periodontal regeneration and adhesion prevention are comprehensively reviewed. Finally, the future perspectives and challenges in the research of multidrug-loaded EFMs are discussed.
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http://dx.doi.org/10.1016/j.jconrel.2020.11.036DOI Listing
February 2021

Angelica Essential Oil Loaded Electrospun Gelatin Nanofibers for Active Food Packaging Application.

Polymers (Basel) 2020 Feb 2;12(2). Epub 2020 Feb 2.

School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China.

The development of food packaging possessing bioactivities which could extend the shelf life of food has gained increased interest in recent years. In this study, gelatin nanofibers with encapsulated angelica essential oil (AEO) were fabricated via electrospinning. The morphology of gelatin/AEO nanofibers was examined by scanning electron microscopy (SEM) and the addition of AEO resulted in the increase of fiber diameter. The proton nuclear magnetic resonance (H-NMR) spectra were measured to confirm the presence of AEO in nanofibers. The hydrophobic property of gelatin nanofibers was also found to be improved with the addition of AEO. The nanofibers incorporated with AEO showed significant antioxidant activity and inhibitory effect against both Gram-negative and Gram-positive bacteria in a concentration dependent manner. Furthermore, the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay demonstrated that the developed gelatin/AEO nanofibers revealed no cytotoxicity effect. Thus, gelatin nanofibers incorporated with AEO can be used as potential food packaging.
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http://dx.doi.org/10.3390/polym12020299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077408PMC
February 2020

Melt electrohydrodynamic 3D printed poly (ε-caprolactone)/polyethylene glycol/roxithromycin scaffold as a potential anti-infective implant in bone repair.

Int J Pharm 2020 Feb 24;576:118941. Epub 2019 Dec 24.

School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China; School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, China. Electronic address:

Implanted scaffold or bone substitute is a common method to treat bone defects. However, the possible bone infection caused by orthopaedic surgery has created a challenging clinical problem and generally invalidate bone repair and regeneration. In this study, a poly (ε-caprolactone) (PCL)/polyethylene glycol (PEG)/roxithromycin (ROX) composite scaffold was prepared via melt electrohydrodynamic (EHD) 3D printing. Fourier transform infrared spectroscopy (FTIR) spectroscopy was performed to verify the existence of PEG and ROX in the scaffolds. By water contact angle measurement, the addition of both PEG and ROX was found to improve the hydrophilicity of the scaffolds. By in vitro drug release assay, the PCL/PEG/ROX scaffolds showed an initial burst drug release and subsequent long-term sustained release behaviour, which is favourable for the prevention and treatment of bone infections. The antibacterial assays against E. coli and S. aureus demonstrated that the composite scaffold with ROX possessed effective antibacterial activity, especially for S. aureus, the main cause of bone infection. The immunostaining and MTT assay with human osteoblast-like cells (MG63) indicated that cells showed good viability and growth on the scaffolds. Therefore, the melt EHD 3D printed PCL/PEG/ROX scaffold could be a promising anti-infective implant for bone tissue engineering.
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http://dx.doi.org/10.1016/j.ijpharm.2019.118941DOI Listing
February 2020

Effects of myo-inositol hexakisphosphate, ferrihydrite coating, ionic strength and pH on the transport of TiO nanoparticles in quartz sand.

Environ Pollut 2019 Sep 4;252(Pt B):1193-1201. Epub 2019 Jun 4.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agriculture University, Wuhan, 430070, China. Electronic address:

Evaluating the fate and transport of nanoparticles (NPs) in the subsurface environment is critical for predicting the potential risks to both of the human health and environmental safety. It is believed that numerous environmental factors conspire to control the transport dynamics of nanoparticles, yet the effects of organic phosphates on nanoparticles transport remain largely unknown. In this work, we quantified the transport process of TiO nanoparticle (nTiO) and their retention patterns in water-saturated sand columns under various myo-inositol hexakisphosphate (IHP) or phosphate (Pi) concentrations (0-180 μM P), ferrihydrite coating fractions (λ, 0-30%), ionic strengths (1-50 mM KCl), and pH values (4-8). The transport of nTiO was enhanced at increased P concentration due to the enhanced colloidal stability. As compared with Pi at the equivalent P level, IHP showed stronger effect on the electrokinetic properties of nTiO particles due to its relatively more negative charge and higher adsorption affinity, thereby facilitating the nTiO transport (and thus reduced retention) in porous media. At the IHP concentration of 5 μM, the retention of nTiO increased with increasing λ and ionic strength, while decreased with pH. In addition, the retention profiles of nTiO showed a typical hyperexponential pattern for most scenarios mainly due to the unfavorable attachment, and can be well described by a hybrid mathematical model that coupled convection dispersion equations with a two-site kinetic model and DLVO theory. These quantitative estimations revealed the importance of IHP on affecting the transport of nTiO typically in phosphorus-enriched environments. It provides new insights into advanced understanding of the co-transport of nanoparticles and phosphorus in natural systems, essential for both nanoparticle exposure and water eutrophication.
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http://dx.doi.org/10.1016/j.envpol.2019.06.008DOI Listing
September 2019

Honey loaded alginate/PVA nanofibrous membrane as potential bioactive wound dressing.

Carbohydr Polym 2019 Sep 8;219:113-120. Epub 2019 May 8.

School of Mechanical and Electric Engineering, Guangzhou University, Guangzhou, 510006, China. Electronic address:

Honey is an ancient natural wound-healing agent and has been reintroduced to modern clinical wound care as it has various bioactivities. In this study, honey was incorporated into an alginate/PVA-based electrospun nanofibrous membrane to develop an efficient wound dressing material. The morphology and chemical composition of the nanofibrous membrane were observed by scanning electron microscopy and characterized via Fourier transform infrared spectroscopy, respectively, demonstrating that honey was successfully introduced to the nanofibers. The nanofibrous membranes with increasing honey content showed enhanced antioxidant activity, suggesting the ability to control the overproduction of reactive oxygen species. Disc diffusion assay and dynamic contact assay proved the antibacterial activity of the honey loaded nanofibers towards Gram-positive bacterium (Staphylococcus aureus) and Gram-negative bacterium (Escherichia coli). The cytotoxicity assay illustrated the non-cytotoxicity and biocompatibility of the nanofibrous membranes. Therefore, the developed honey/alginate/PVA nanofibrous membranes are promising for wound dressings.
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http://dx.doi.org/10.1016/j.carbpol.2019.05.004DOI Listing
September 2019

Electrospun Gelatin Nanofibers Encapsulated with Peppermint and Chamomile Essential Oils as Potential Edible Packaging.

J Agric Food Chem 2019 Feb 15;67(8):2227-2234. Epub 2019 Feb 15.

School of Mechanical and Electric Engineering , Guangzhou University , Guangzhou , Guangdong 510006 , People's Republic of China.

Natural and edible materials have attracted increasing attention in food packaging, which could overcome the serious environmental issues caused by conventional non-biodegradable synthetic packaging. In this work, gelatin nanofibers incorporated with two kinds of essential oil (EO), peppermint essential oil (PO) and chamomile essential oil (CO), were fabricated by electrospinning for potential edible packaging application. Electron microscopy showed that smooth and uniform morphology of the gelatin/EOs was obtained, and the diameter of nanofibers was mostly enlarged with the increase of the EO content. The proton nuclear magnetic resonance spectrum confirmed the existence of PO and CO in nanofibers after electrospinning. The addition of EOs led to an enhancement of the water contact angle of nanofibers. The antioxidant activity was significantly improved for the nanofibers loaded with CO, while the antibacteria activity against Escherichia coli and Staphylococcus aureus was better for the fibers with PO addition. The combination of half PO and half CO in nanofibers compensated for their respective limitations and exhibited optimum bioactivities. Finally, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay with NIH-3T3 fibroblasts demonstrated the absence of cytotoxicity of the gelatin/EO nanofibers. Thus, our studies suggest that the developed gelatin/PO/CO nanofiber could be a promising candidate for edible packaging.
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http://dx.doi.org/10.1021/acs.jafc.8b06226DOI Listing
February 2019

Comparative genomics for non-O1/O139 Vibrio cholerae isolates recovered from the Yangtze River Estuary versus V. cholerae representative isolates from serogroup O1.

Mol Genet Genomics 2019 Apr 28;294(2):417-430. Epub 2018 Nov 28.

Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), China Ministry of Agriculture, College of Food Science and Technology, Shanghai Ocean University, Shanghai, People's Republic of China.

Vibriocholerae, which is autochthonous to estuaries worldwide, can cause human cholera that is still pandemic in developing countries. A number of V. cholerae isolates of clinical and environmental origin worldwide have been subjected to genome sequencing to address their phylogenesis and bacterial pathogenesis, however, little genome information is available for V. cholerae isolates derived from estuaries, particularly in China. In this study, we determined the complete genome sequence of V. cholerae CHN108B (non-O1/O139 serogroup) isolated from the Yangtze River Estuary, China and performed comparative genome analysis between CHN108B and other eight representative V. cholerae isolates. The 4,168,545-bp V. cholerae CHN108B genome (47.2% G+C) consists of two circular chromosomes with 3,691 predicted protein-encoding genes. It has 110 strain-specific genes, the highest number among the eight representative V. cholerae whole genomes from serogroup O1: there are seven clinical isolates linked to cholera pandemics (1937-2010) and one environmental isolate from Brazil. Various mobile genetic elements (such as insertion sequences, prophages, integrative and conjugative elements, and super-integrons) were identified in the nine V. cholerae genomes of clinical and environmental origin, indicating that the bacterium undergoes extensive genetic recombination via lateral gene transfer. Comparative genomics also revealed different virulence and antimicrobial resistance gene patterns among the V. cholerae isolates, suggesting some potential virulence factors and the rising development of resistance among pathogenic V. cholerae. Additionally, draft genome sequences of multiple V. cholerae isolates recovered from the Yangtze River Estuary were also determined, and comparative genomics revealed many genes involved in specific metabolism pathways, which are likely shaped by the unique estuary environment. These results provide additional evidence of V. cholerae genome plasticity and will facilitate better understanding of the genome evolution and pathogenesis of this severe water-borne pathogen worldwide.
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http://dx.doi.org/10.1007/s00438-018-1514-6DOI Listing
April 2019

Glabridin, an isoflavan from licorice root, ameliorates imiquimod-induced psoriasis-like inflammation of BALB/c mice.

Int Immunopharmacol 2018 Jun 14;59:243-251. Epub 2018 Apr 14.

Institute of Natural Medicine & Green Chemistry, School of Chemical Engineering and Light Industry, Guandong University of Technology, Guangzhou, 510006, China. Electronic address:

In this paper, we investigate the effect of glabridin (Glab) on psoriasis. We observed that Glab significantly suppressed the levels of nitric oxide (NO), NF-κB subunit p65, interleukin (IL)-6, and IL-1β in lipopolysaccharide (LPS)-stimulated HaCaT cells. In addition, Glab treatment reduced the expression of IL-17A, IL-22, and IL-23 in TNF-α-stimulated-HaCaT cells. These findings prompted us to test whether Glab could be used to treat psoriasis in vivo. The effects of Glab on PASI scores, histopathological changes, oxidative/anti-oxidative indexes and pro-inflammatory cytokines in IMQ-induced mice were investigated. The results indicated that Glab could reduce the PASI scores and ameliorate the deteriorating histopathology. Interestingly, RT-PCR revealed that Glab significantly decreased the mRNA expression of p65, IL-6, IL-1β, IL-17A, IL-22, and IL-23. These results were confirmed by Western blot analysis and immunohistochemistry staining. In conclusion, our present study revealed that Glab had beneficial effects on psoriasis, and the underlying mechanism may be associated with the downregulation of pro-inflammatory cytokines and the improvement of antioxidant status. Hence, Glab is a promising candidate molecule for development of effective psoriasis therapies.
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http://dx.doi.org/10.1016/j.intimp.2018.04.018DOI Listing
June 2018

iAPSL-IF: Identification of Apoptosis Protein Subcellular Location Using Integrative Features Captured from Amino Acid Sequences.

Int J Mol Sci 2018 Apr 13;19(4). Epub 2018 Apr 13.

Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), China Ministry of Agriculture, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Apoptosis proteins (APs) control normal tissue homeostasis by regulating the balance between cell proliferation and death. The function of APs is strongly related to their subcellular location. To date, computational methods have been reported that reliably identify the subcellular location of APs, however, there is still room for improvement of the prediction accuracy. In this study, we developed a novel method named iAPSL-IF (identification of apoptosis protein subcellular location-integrative features), which is based on integrative features captured from Markov chains, physicochemical property matrices, and position-specific score matrices (PSSMs) of amino acid sequences. The matrices with different lengths were transformed into fixed-length feature vectors using an auto cross-covariance (ACC) method. An optimal subset of the features was chosen using a recursive feature elimination (RFE) algorithm method, and the sequences with these features were trained by a support vector machine (SVM) classifier. Based on three datasets ZD98, CL317, and ZW225, the iAPSL-IF was examined using a jackknife cross-validation test. The resulting data showed that the iAPSL-IF outperformed the known predictors reported in the literature: its overall accuracy on the three datasets was 98.98% (ZD98), 94.95% (CL317), and 97.33% (ZW225), respectively; the Matthews correlation coefficient, sensitivity, and specificity for several classes of subcellular location proteins (e.g., membrane proteins, cytoplasmic proteins, endoplasmic reticulum proteins, nuclear proteins, and secreted proteins) in the datasets were 0.92-1.0, 94.23-100%, and 97.07-100%, respectively. Overall, the results of this study provide a high throughput and sequence-based method for better identification of the subcellular location of APs, and facilitates further understanding of programmed cell death in organisms.
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http://dx.doi.org/10.3390/ijms19041190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979326PMC
April 2018

Three-dimensional prostate tumor model based on a hyaluronic acid-alginate hydrogel for evaluation of anti-cancer drug efficacy.

J Biomater Sci Polym Ed 2017 Oct 8;28(14):1603-1616. Epub 2017 Jun 8.

a Institute of Natural Medicinal Chemistry and Green Chemistry, Guangdong University of Technology , Guangdong , China.

In vitro cell-based assays are widely applied to evaluate anti-cancer drug efficacy. However, the conventional approaches are mostly based on two-dimensional (2D) culture systems, making it difficult to recapitulate the in vivo tumor scenario because of spatial limitations. Here, we develop an in vitro three-dimensional (3D) prostate tumor model based on a hyaluronic acid (HA)-alginate hybrid hydrogel to bridge the gap between in vitro and in vivo anticancer drug evaluations. In situ encapsulation of PCa cells was achieved by mixing HA and alginate aqueous solutions in the presence of cells and then crosslinking with calcium ions. Unlike in 2D culture, cells were found to aggregate into spheroids in a 3D matrix. The expression of epithelial to mesenchyme transition (EMT) biomarkers was found to be largely enhanced, indicating an increased invasion and metastasis potential in the hydrogel matrix. A significant up-regulation of proangiogenic growth factors (IL-8, VEGF) and matrix metalloproteinases (MMPs) was observed in 3D-cultured PCa cells. The results of anti-cancer drug evaluation suggested a higher drug tolerance within the 3D tumor model compared to conventional 2D-cultured cells. Finally, we found that the drug effect within the in vitro 3D cancer model based on HA-alginate matrix exhibited better predictability for in vivo drug efficacy.
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http://dx.doi.org/10.1080/09205063.2017.1338502DOI Listing
October 2017

Induction and differentiation of human induced pluripotent stem cells into functional cardiomyocytes on a compartmented monolayer of gelatin nanofibers.

Nanoscale 2016 Aug 14;8(30):14530-40. Epub 2016 Jul 14.

Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités - UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France.

Extensive efforts have been devoted to develop new substrates for culture and differentiation of human induced pluripotent stem cells (hiPSCs) toward cardiac cell-based assays. A more exciting prospect is the construction of cardiac tissue for robust drug screening and cardiac tissue repairing. Here, we developed a patch method by electrospinning and crosslinking of monolayer gelatin nanofibers on a honeycomb frame made of poly(ethylene glycol) diacrylate (PEGDA). The monolayer of the nanofibrous structure can support cells with minimal exogenous contact and a maximal efficiency of cell-medium exchange whereas a single hiPSC colony can be uniformly formed in each of the honeycomb compartments. By modulating the treatment time of the ROCK inhibitor Y-27632, the shape of the hiPSC colony could be controlled from a flat layer to a hemisphere. Afterwards, the induction and differentiation of hiPSCs were achieved on the same patch, leading to a uniform cardiac layer with homogeneous contraction. This cardiac layer could then be used for extracellular recording with a commercial multi-electrode array, showing representative field potential waveforms of matured cardiac tissues with appropriate drug responses.
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http://dx.doi.org/10.1039/c6nr04545fDOI Listing
August 2016

Effective motor neuron differentiation of hiPSCs on a patch made of crosslinked monolayer gelatin nanofibers.

J Mater Chem B 2016 May 25;4(19):3305-3312. Epub 2016 Apr 25.

Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités - UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France.

Human induced pluripotent stem cells (hiPSCs) are differentiated into mature motor neurons by using a culture patch made of crosslinked monolayer gelatin nanofibers. Compared to the conventional culture dish method, the patch method is more effective for culture and differentiation of stem cells, because cells are supported by a net-like structure made of crosslinked monolayer nanofibers instead of a planar substrate. The pores of the net-like structure have sizes smaller than those of cells but large enough to minimize the exogenous cell-material contact and to increase the permeability as well as the efficiency of cell-cell interactions. As expected, the differentiated hiPSCs showed the up-regulation of the expression of neuron specific proteins and the signature of matured motor neurons, allowing plug-and-play with a commercial multi-electrode array for neuron spike recording.
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http://dx.doi.org/10.1039/c6tb00351fDOI Listing
May 2016

Microfluidic device with integrated microfilter of conical-shaped holes for high efficiency and high purity capture of circulating tumor cells.

Sci Rep 2014 Aug 13;4:6052. Epub 2014 Aug 13.

1] Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités - UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France [2] Institute for Integrated Cell-Material Science, Kyoto University, Kyoto 606-8507, Japan [3] Institute for Interdisciplinary Research, Jianghan University, 430056 Wuhan, China.

Capture of circulating tumor cells (CTCs) from peripheral blood of cancer patients has major implications for metastatic detection and therapy analyses. Here we demonstrated a microfluidic device for high efficiency and high purity capture of CTCs. The key novelty of this approach lies on the integration of a microfilter with conical-shaped holes and a micro-injector with cross-flow components for size dependent capture of tumor cells without significant retention of non-tumor cells. Under conditions of constant flow rate, tumor cells spiked into phosphate buffered saline could be recovered and then cultured for further analyses. When tumor cells were spiked in blood of healthy donors, they could also be recovered at high efficiency and high clearance efficiency of white blood cells. When the same device was used for clinical validation, CTCs could be detected in blood samples of cancer patients but not in that of healthy donors. Finally, the capture efficiency of tumor cells is cell-type dependent but the hole size of the filter should be more closely correlated to the nuclei size of the tumor cells. Together with the advantage of easy operation, low-cost and high potential of integration, this approach offers unprecedented opportunities for metastatic detection and cancer treatment monitoring.
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http://dx.doi.org/10.1038/srep06052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365311PMC
August 2014
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