Publications by authors named "Ya-Li Yang"

39 Publications

Inhibition of Improve Lipid Metabolism Disorder in Dihydrotestosterone-Induced Hyperandrogenic Mice.

Front Cell Dev Biol 2021 24;9:669189. Epub 2021 May 24.

Center for Energy Metabolism and Reproduction, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Hyperandrogenism is a key pathological feature of polycystic ovarian syndrome (PCOS). Excess androgen can lead to PCOS-like cell hypertrophy in the ovaries and adipose tissue of rodents. Here, we established a dihydrotestosterone (DHT)-induced hyperandrogenic mouse model to analyze the differences in gene expression and signaling pathways of the ovaries and gonad fat pads of mice treated with or without DHT by RNA microarray analysis. From the results, we focused on the overlapping differentially expressed gene--and the major differentially enriched signaling pathway-lipid metabolism. We employed DHT-induced mouse ovarian stromal cell, adipogenic 3T3-L1 cell and hepatic cell line NCTC1469 models to investigate whether androgens directly mediate lipid accumulation and hypertrophy. We found that DHT increased lipid droplet accumulation in ovarian stromal cells and adipogenic 3T3-L1 cells but not NCTC1469 cells. DHT significantly altered stromal cell cholesterol metabolism and steroidogenesis, as indicated by changes in cholesterol levels and the expression of related genes, but these effects were not observed in 3T3-L1 cells. Moreover, expression was significantly increased in ovaries and gonadal fat pads of DHT-treated mice, and inhibition alleviated DHT-induced excess lipid accumulation and hypertrophy of ovarian stromal cells and adipogenic 3T3-L1 cells, even improved lipid metabolism in overnourished NCTC1469 cells. Our results indicate that plays important roles in the pathogenesis of DHT-induced lipid metabolism disorder and the hypertrophy of ovarian stromal cells and adipocytes.
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http://dx.doi.org/10.3389/fcell.2021.669189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181728PMC
May 2021

Inhibitory Effect of Astragalus Polysaccharide Combined with Cisplatin on Cell Cycle and Migration of Nasopharyngeal Carcinoma Cell Lines.

Biol Pharm Bull 2021 Jul 29;44(7):926-931. Epub 2021 Apr 29.

School of Medicine, Jiaying University.

Background Astragalus polysaccharide (APS) had shown great promise in anti-tumour activities in our previous studies. The present study was designed to investigate whether APS has synergistic effect with cisplatin on the growth-inhibitory of human nasopharyngeal carcinoma cell lines and the possible mechanism. Methods Here, nasopharyngeal carcinoma cell lines (CNE-1) were divided into CNE-1 group, Cisplatin treatment group (2 µg/mL Cisplatin), APS treatment group (200 µg/mL APS) and combination group (2 µg/mL Cisplatin and 200 µg/mL APS). The proliferation inhibition rate of CNE-1 cells was determined by Cell Counting Kit-8 (CCK-8) method after treatment with different concentrations of APS for 24, 48, and 72 h. Apoptosis rates and cell cycle retardation of cells were detected by flow cytometry. Cell migration and invasion was evaluated by transwell assay. Western blotting and quantitative (q)RT-PCR were performed to detect the expression of Bcl-2, Bax, caspase-3, matrix metalloproteinase-2 (MMP-2), p53 and matrix metalloproteinase-9 (MMP-9) proteins in CNE-1 cells. Results APS have an inhibition on the proliferation of CNE-1 cells with time and dose dependence manner. Both the APS and combination therapy could promote apoptosis of CNE-1 cells, with the count of cells increased in G0/G1 and S phase while decreased in G2/M phase, and inhibited the migration and invasion of CNE-1 cells. Moreover, co-administration of Cisplatin and APS was more efficacious for the antitumor effect than either agent alone, as evidenced by the significant decrease in MMP-9 level and increase in p53. Conclusion APS, in combination with cisplatin, had significantly synergistic growth-inhibitory effect on nasopharyngeal carcinoma cell lines, which may be related to cell cycle and migration induction.
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http://dx.doi.org/10.1248/bpb.b20-00959DOI Listing
July 2021

Higher versus lower mean arterial pressure target management in older patients having non-cardiothoracic surgery: A prospective randomized controlled trial.

J Clin Anesth 2021 May 6;69:110150. Epub 2021 Jan 6.

Department of Anesthesiology, Shenzhen People's Hospital, Shenzhen, China; The Second Clinical Medical College, Jinan University, Shenzhen, China; First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China. Electronic address:

Study Objective: This study aimed to evaluate the effects of low versus high mean arterial pressure (MAP) levels on the incidence of postoperative delirium during non-cardiothoracic surgery in older patients.

Design: Multicenter, randomized, parallel-controlled, open-label, and assessor-blinded clinical trial.

Setting: University hospital.

Patients: Three hundred twenty-two patients aged ≥65 with an American Society of Anesthesiologists physical status of I-II who underwent non-cardiothoracic surgery with general anaesthesia.

Interventions: Participants were randomly assigned into a low-level MAP (60-70 mmHg) or high-level MAP (90-100 mmHg) group during general anaesthesia. The study was conducted from November 2016 to February 2020. Participants were older patients having non-cardiothoracic surgery. The follow-up period ranged from 1 to 7 days after surgery. The primary outcome was the incidence of postoperative delirium.

Main Results: In total, 322 patients were included and randomized; 298 completed in-hospital delirium assessments [median (interquartile range) age, 73 (68-77) years; 173 (58.1%) women]. Fifty-four (18.1%) patients total, including 36 (24.5%) and 18 (11.9%) in the low-level and high-level MAP groups [relative risk (RR) 0.48, 95% confidence interval (CI) 0.25 to 0.87, P = 0.02], respectively, experienced postoperative delirium. The adjusted RR was 0.34 (95% CI 0.16 to 0.70, P < 0.01) in the multiple regression analysis. High-level MAP was associated with a shorter delirium span and a higher intraoperative urine volume than low-level MAP.

Conclusions: In older patients during non-cardiothoracic surgery, high-level blood pressure management might help reduce the incidence of postoperative delirium.
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http://dx.doi.org/10.1016/j.jclinane.2020.110150DOI Listing
May 2021

Spleen tyrosine kinase expression and its correlation with necrosis and high-risk histopathologic features in retinoblastoma.

Chin Med J (Engl) 2020 Jul;133(14):1752-1753

Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.

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http://dx.doi.org/10.1097/CM9.0000000000000889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401740PMC
July 2020

Modified Anterior Tibial Artery Perforator-Pedicled Propeller Flap for Soft-Tissue Coverage of the Ankle and Heel.

World J Surg 2020 07;44(7):2237-2242

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.

Background: The ankle and heel are challenging regions to reconstruct functionally. Here, we explored the feasibility and clinical outcomes of a modified anterior tibial artery perforator-pedicled propeller flap for the repair of soft-tissue defects of the ankle and heel.

Patients And Methods: Between January 2013 and December 2015, 12 patients with soft-tissue defects of the ankle and/or heel underwent reconstructive surgery that included our flap technique. The flaps measured 20 × 8 cm to 7 × 4 cm. A hand-held Doppler was used to identify a proper constant perforator in the distal ankle. In each case, the base of the flap was well preserved. The flap was transposed (180° rotation) to reach and cover the defect.

Results: The average follow-up time was 13 months (10-28 months). We observed good texture matches and contour in all of the flaps. All patients could walk and wear normal footwear. All but one flap survived completely without complications. Partial loss was observed in one patient, and the necrotic region was healed with secondary intention.

Conclusion: Our modified anterior tibial artery free-style perforator-pedicled propeller flap provides a novel option for functional ankle and heel reconstruction.

Level Of Evidence: Level IV.
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http://dx.doi.org/10.1007/s00268-020-05452-yDOI Listing
July 2020

Removal of heavy metal ions and anionic dyes from aqueous solutions using amide-functionalized cellulose-based adsorbents.

Carbohydr Polym 2020 Feb 14;230:115619. Epub 2019 Nov 14.

Institute of Agricultural Facilities and Equipment, Jiangsu Academy of Agricultural Sciences, China; Key Laboratory for Protected Agricultural Engineering in the Middle and Lower Reaches of Yangtze River, Ministry of Agriculture, China; School of Materials Science & Engineering, Jiangsu University, China. Electronic address:

An efficient, ecofriendly, amide-functionalized cellulose-based porous adsorbent was synthesized by a cross-linking reaction between cellulose filament fibers and bisacrylamide at room temperature. This process is simple, fast and inexpensive, and has significant potential for industrial applications. The prepared material has numerous adsorption sites, resulting in the highly efficient removal of anionic dyes and copper ions from aqueous media. The maximum adsorption capacities of this cellulose-based adsorbent for the dyes Acid Black 1 and Acid Red 18 and for copper ions were 751.8, 417.9, and 51.3 mg g, respectively. Regeneration experiments showed that the removal efficiencies for all model pollutants remained above 92 % after five consecutive recycling trials. These results indicate that amide-functionalized cellulose-based adsorbents could possibly be used to treat industrial wastewaters.
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http://dx.doi.org/10.1016/j.carbpol.2019.115619DOI Listing
February 2020

Removal of DHT can relieve polycystic ovarian but not metabolic abnormalities in DHT-induced hyperandrogenism in mice.

Reprod Fertil Dev 2019 Sep;31(10):1597-1606

Research Laboratory for Reproductive Health, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; and Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; and Corresponding author. Email:

Polycystic ovary syndrome (PCOS) is an endocrine disorder with a high prevalence in women of childbearing age. To date, there is no method of efficiently diagnosing PCOS and curing it completely because its pathomechanism remains unclear. Here, we investigated whether metabolic abnormalities maintain the hyperandrogenism and PCOS-like ovaries and whether the symptoms induced by excess androgen are treatable. We ceased the abnormal dihydrotestosterone (DHT) stimulation to determine changes in PCOS-like mice. After ceasing DHT stimulation, the ovarian morphology and gene expression recovered from the DHT-stimulated status. However, after cessation of DHT stimulation, the hypertrophy of adipose tissues and hepatic steatosis were not significantly restored, and fat accumulation-related gene expression and serum metabolic markers in the mice were altered. These findings showed that the reproductive dysfunction was obviously relieved, but because the metabolic abnormalities were not relieved after the cessation of excess androgen for 30 days, it appears that the latter may not maintain the former.
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http://dx.doi.org/10.1071/RD18459DOI Listing
September 2019

Antifungal Susceptibility Profile of Candida Albicans Isolated from Vulvovaginal Candidiasis in Xinjiang Province of China.

Mycopathologia 2019 Jun 9;184(3):413-422. Epub 2019 Apr 9.

The People's Hospital of Suzhou National New & Hi-Tech Industrial Development Zone, Suzhou, Jiangsu, China.

We investigated the antifungal susceptibility profiles of 207 independent Candida albicans strains isolated from patients with vulvovaginal candidiasis (VVC) in Xinjiang Province of China. Using CLSI M27-A3 and M27-S4 guidelines, anidulafungin and micafungin were the most active drugs against C. albicans showing an MIC/MIC corresponding to 0.016/0.0313 µg/mL, followed by caspofungin (0.25/0.25 µg/mL), posaconazole (0.125/0.5 µg/mL), ravuconazole (0.063/1 µg/mL), itraconazole (0.125/1 µg/mL), amphotericine B (0.5/1 µg/mL), isavuconazole (0.063/2 µg/mL), 5-flucytosine (1/2 µg/mL), voriconazole (0.125/4 µg/mL), and fluconazole (0.5/4 µg/mL). 96.1% (199)-100.0% (207) isolates were sensitive to the three echinocandins tested, amphotericine B and 5-flucytosine. The in vitro activity of triazoles against all isolates tested was variable; itraconazole and voriconazole had reduced the activity to almost half of the isolates (55.1% (114) and 51.2% (106) susceptible, respectively). Fluconazole was active against 76.3% (158) isolates tested. The new triazoles ravuconazole, isavuconazole and posaconazole showed good in vitro potency against 89.9% (186)-95.2% (197) of isolates with the geometric mean MIC (µg/mL) of 0.10, 0.12 and 0.14 µg/mL, respectively. In conclusion, our study indicates that for effective management of systemic candidiasis in Xinjiang Province of China, it is important to determine the susceptibility profiles of isolated C. albicans from patients with VVC.
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http://dx.doi.org/10.1007/s11046-018-0305-2DOI Listing
June 2019

Phenotypic and Molecular Characterisation of Staphylococcus Aureus with Reduced Vancomycin Susceptibility Derivated in Vitro.

Open Med (Wars) 2018 22;13:475-486. Epub 2018 Oct 22.

Department of Medical Microbiology and Parasitology, College of Basic Medical Sciences, China Medical University, Shenyang, PR China.

Vancomycin has been the primary agent used to treat serious Methicillin-resistant (MRSA) infection for many years. However, the rise of MRSA infection rates and the extensive use of vancomycin have led to the emergence of reduced vancomycin susceptibility. Therefore, four typical ( strains from different clinical specimens were derivated by vancomycin in vitro to better clarify their phenotypic and molecular characteristics. Some experiments, such as stepwise selection of vancomycin-resistant strains, pulsed-field gel electrophoresis (PFGE), antimicrobial susceptibility test, population analysis profile-area under the curve (PAP-AUC), molecular typing, transmission electron microscopy, δ-hemolysin expression, autolysis assay, biofilm assay and quantitative real-time polymerase chain reaction (qPCR) for gene expression were carried out to compare the derivated bacteria with their parental strains. Results showed that the observed phenotypes of vancomycin-resistant strains such as hemolysin, autolysis and biofilm significantly reduced, which were associated with vancomycin resistance capability of the selected strain. The changes of phenotype and regulatory genes expression were inversely proportional to the vancomycin minimum inhibitory concentration (MICvan). Most heterogeneous vancomycin intermediate (hVISA) or VISA strains belonged to type t570 and group II. In summary, the clinical isolated vancomycin susceptible (VSSA), hVISA and VISA could be derivated into high vancomycin-resistant VISA in vitro, but it was difficult for them to develop into vancomycin resistant (VRSA). VISA and hVISA could gradually adapt to the environment with the vancomycin concentration that continuously elevates.
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http://dx.doi.org/10.1515/med-2018-0071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6227741PMC
October 2018

Global mRNA and Long Non-Coding RNA Expression in the Placenta and White Adipose Tissue of Mice Fed a High-Fat Diet During Pregnancy.

Cell Physiol Biochem 2018 13;50(6):2260-2271. Epub 2018 Nov 13.

Laboratory for Reproductive Health, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Background/aims: Gestational diabetes mellitus (GDM) is a common complication of pregnancy, but the mechanisms underlying the disorders remain unclear. The study aimed to identify mRNA and long non-coding RNA (lncRNA) profiles in placenta and gonadal fat of pregnant mice fed a high-fat diet and to investigate the transcripts and pathways involved in the development of gestational diabetes mellitus.

Methods: Deep and broad transcriptome profiling was performed to assess the expression of mRNAs and lncRNAs in placenta and gonadal fat from 3 mice fed an HFD and chow during pregnancy. Then, differentially expressed mRNAs and lncRNAs were validated by quantitative real-time PCR. The function of the differentially expressed mRNAs was determined by pathway and Gene Ontology (GO) analyses, and the physical or functional relationships between the lncRNAs and the corresponding mRNAs were determined.

Results: Our study revealed that 82 mRNAs and 52 lncRNAs were differentially expressed in the placenta of mice fed an HFD during pregnancy, and 202 mRNAs and 120 lncRNAs were differentially expressed in gonadal fat. GO and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed differentially expressed mRNAs of placenta were closely related to extracellular matrix interactions, digestion, adhesion, and metabolism, whereas the differentially expressed mRNAs in adipose tissue were related to metabolic and insulin signalling pathways. The gene network demonstrated that Actg2, Cnfn, Muc16, Serpina3k, NONMMUT068202, and NONMMUT068203, were the core of the network in placental tissue, and the genes Tkt, Acss2, and Elovl6 served as the core of the network in gonadal fat tissue.

Conclusion: These newly identified key genes and pathways in mice might provide valuable information regarding the pathogenesis of GDM and might be used to improve early diagnosis, prevention, drug design, and clinical treatment.
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http://dx.doi.org/10.1159/000495086DOI Listing
December 2018

Deficiency of Gpr1 improves steroid hormone abnormality in hyperandrogenized mice.

Reprod Biol Endocrinol 2018 May 24;16(1):50. Epub 2018 May 24.

Research Laboratory for Reproductive Health, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Background: Polycystic ovary syndrome (PCOS) is a complex genetic disease with multifarious phenotypes. Many researches use dehydroepiandrosterone (DHEA) to induce PCOS in pubertal mouse models. The aim of this study was to investigate the role of GPR1 in dehydroepiandrosterone (DHEA)-induced hyperandrogenized mice.

Methods: Prepubertal C57BL/6 mice (25 days of age) and Gpr1-deficient mice were each divided into two groups and injected daily with sesame oil with or without DHEA (6 mg/100 g) for 21 consecutive days. Hematoxylin and eosin (H&E) staining was performed to determine the characteristics of the DHEA-treated ovaries. Real-time PCR was used to examine steroid synthesis enzymes gene expression. Granulosa cell was cultured to explore the mechanism of DHEA-induced, GPR1-mediated estradiol secretion.

Results: DHEA treatment induced some aspects of PCOS in wild-type mice, such as increased body weight, elevated serum testosterone, increased number of small, cystic, atretic follicles, and absence of corpus luteum in ovaries. However, Gpr1 deficiency significantly attenuated the DHEA-induced weight gain and ovarian phenotype, improving steroidogenesis in ovaries and estradiol synthesis in cultured granulosa cells, partially through mTOR signaling.

Conclusions: In conclusion, Gpr1 deficiency leads to the improvement of steroid synthesis in mice hyperandrogenized with DHEA, indicating that GPR1 may be a therapeutic target for DHEA-induced hyperandrogenism.
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http://dx.doi.org/10.1186/s12958-018-0363-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5968470PMC
May 2018

Five New Alkaloids from the Roots of Sophora flavescens.

Chem Biodivers 2018 Mar;15(3):e1700577

Guangdong Province Hospital of TCM, Guangzhou, 510000, P. R. China.

Five new quinolizidine alkaloids, including three sparteine-type alkaloids (1 - 3) and two cytisine-type alkaloids (4 and 5), along with four known ones, were isolated from the roots of Sophora flavescens. Their structures were determined by extensive spectroscopic techniques including IR, UV, NMR, and HR-ESI-MS. All the compounds were evaluated for their antibacterial activities against Staphylococcus aureus and Escherichia coli.
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http://dx.doi.org/10.1002/cbdv.201700577DOI Listing
March 2018

The role of GPR1 signaling in mice corpus luteum.

J Endocrinol 2016 07 5;230(1):55-65. Epub 2016 May 5.

Research Laboratory for Reproductive HealthShenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China

Chemerin, a chemokine, plays important roles in immune responses, inflammation, adipogenesis, and carbohydrate metabolism. Our recent research has shown that chemerin has an inhibitory effect on hormone secretion from the testis and ovary. However, whether G protein-coupled receptor 1 (GPR1), the active receptor for chemerin, regulates steroidogenesis and luteolysis in the corpus luteum is still unknown. In this study, we established a pregnant mare serum gonadotropin-human chorionic gonadotropin (PMSG-hCG) superovulation model, a prostaglandin F2α (PGF2α) luteolysis model, and follicle and corpus luteum culture models to analyze the role of chemerin signaling through GPR1 in the synthesis and secretion of gonadal hormones during follicular/luteal development and luteolysis. Our results, for the first time, show that chemerin and GPR1 are both differentially expressed in the ovary over the course of the estrous cycle, with highest levels in estrus and metestrus. GPR1 has been localized to granulosa cells, cumulus cells, and the corpus luteum by immunohistochemistry (IHC). In vitro, we found that chemerin suppresses hCG-induced progesterone production in cultured follicle and corpus luteum and that this effect is attenuated significantly by anti-GPR1 MAB treatment. Furthermore, when the phosphoinositide 3-kinase (PI3K) pathway was blocked, the attenuating effect of GPR1 MAB was abrogated. Interestingly, PGF2α induces luteolysis through activation of caspase-3, leading to a reduction in progesterone secretion. Treatment with GPR1 MAB blocked the PGF2α effect on caspase-3 expression and progesterone secretion. This study indicates that chemerin/GPR1 signaling directly or indirectly regulates progesterone synthesis and secretion during the processes of follicular development, corpus luteum formation, and PGF2α-induced luteolysis.
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http://dx.doi.org/10.1530/JOE-15-0521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5064765PMC
July 2016

Off-label photodynamic therapy for recalcitrant facial flat warts using topical 5-aminolevulinic acid.

Lasers Med Sci 2016 Jul 8;31(5):929-36. Epub 2016 Apr 8.

Department of Dermatology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China.

The facial flat wart (verruca plana) is one of the most common reasons for dermatology and primary care visits. Although there are many therapeutic modalities, no single therapy has been proven to be completely curative. Case reports and uncontrolled studies suggested that photodynamic therapy (PDT) with topical 5-aminolevulinic acid (ALA) can effectively treat recalcitrant facial flat warts. A total of 12 patients with recalcitrant facial flat warts were enrolled in the study. ALA gel (10 %) was applied topically to lesions and incubated for 3 h. The lesions were irradiated by an LED light of 630 ± 10 nm at dose levels of 60-100 mW/cm. Clinical assessment was conducted before and after every treatment for up to 24 weeks. Among the ten patients completing three sessions of ALA-PDT, five had complete lesions clearance, and the other five patients were significantly improved. At the 24-week follow-up, the average effective rate was 88.8 %, with no recurrences. No significant side effects were reported. A low-dose topical ALA-PDT regimen using 10 % ALA, 3 h incubation, and a red light source for three treatment sessions are suggested as the optimal scheme for the treatment of recalcitrant flat warts on the face in Chinese patients. Superior efficacy is found in elevated or active period lesions with mild side effects.
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http://dx.doi.org/10.1007/s10103-016-1925-8DOI Listing
July 2016

Heparanase mRNA and Protein Expression Correlates with Clinicopathologic Features of Gastric Cancer Patients: a Meta- analysis.

Asian Pac J Cancer Prev 2015 ;16(18):8653-8

School of Medicine Technology, Gansu University of Traditional Chinese Medicine, Lanzhou, China E-mail :

Background: Heparanase is believed to be involved in gastric carcinogenesis. However, the clinicopathologic features of gastric cancer with high heparanase expression remain unclear.

Aim: The purpose of this study was to comprehensively and quantitatively summarize available evidence for the use of heparanase mRNA and protein expression to evaluate the clinicopathological associations in gastric cancer in Asian patients by meta-analysis.

Materials And Methods: Relevant articles listed in MEDLINE, CNKI and the Cochrane Library databases up to MARCH 2015 were searched by use of several keywords in electronic databases. A meta-analysis was performed to clarify the impact of heparanase mRNA and protein on clinicopathological parameters in gastric cancer. Combined ORs with 95%CIs were calculated by Revman 5.0, and publication bias testing was performed by stata12.0.

Results: A total of 27 studies which included 3,891 gastric cancer patients were combined in the final analysis. When stratifying the studies by the pathological variables of heparanase mRNA expression, the depth of invasion (633 patients) (OR=4.96; 95% CI=2.38-1.37; P<0.0001), lymph node metastasis (639 patients) (OR=6.22; 95%CI=2.70-14.34, P<0.0001), and lymph node metastasis (383 patients) (OR=6.85; 95% CI=2.04-23.04; P=0.002) were all significant. When stratifying the studies by the pathological variables of heparanase protein expression, this was the case for depth of invasion (1250 patients) (OR=2.76; 95% CI=1.52-5.03; P=0.0009), lymph node metastasis (1178 patients) (OR=4.79 ; 95% CI=3.37-6.80, P<0.00001), tumor size (727 patients) (OR=2.06 ; 95% CI=1.31-3.23; P=0.002) (OR=2.61; 95% CI=2.09-3.27; P=0.000), and TNM stage (1233 patients) (OR=6.85; 95% CI=2.04-23.04; P=0.002). Egger's tests suggested publication bias for depth of invasion, lymph node metastasis, lymph node metastasis and tumor size of heparanase mRNA and protein expression.

Conclusions: This meta- analysis suggests that higher heparanase expression in gastric cancer is associated with clinicopathologic features of depth of invasion, lymph node metastasis and TNM stage at mRNA and protein levels, and of tumor size only at the protein level. Egger's tests suggested publication bias for these clinicopathologic features of heparanase mRNA and protein expression, and which may be caused by shortage of relevant studies. As a result, although abundant reports showed heparanase may be associated with clinicopathologic features in gastric cancer, this meta-analysis indicates that more strict studies were needed to evaluate its clinicopathologic significance.
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http://dx.doi.org/10.7314/apjcp.2015.16.18.8653DOI Listing
October 2016

[Leaching Remediation of Copper and Lead Contaminated Lou Soil by Saponin Under Different Conditions].

Huan Jing Ke Xue 2015 Apr;36(4):1445-52

In order to investigate the leaching remediation effect of the eco-friendly biosurfactant saponin for Cu and Pb in contaminated Lou soil, batch tests method was used to study the leaching effect of saponin solution on single Cu, Pb contaminated Lou soil and mixed Cu and Pb contaminated Lou soil under different conditions such as reaction time, mass concentration of saponin, pH, concentration of background electrolyte and leaching times. The results showed that the maximum leaching removal effect of Cu and Pb in contaminated Lou soil was achieved by complexation of the heavy metals with saponin micelle, when the mass concentration of saponin solution was 50 g x L(-1), pH was 5.0, the reaction time was 240 min, and there was no background electrolyte. In single and mixed contaminated Lou soil, the leaching percentages of Cu were 29.02% and 25.09% after a single leaching with 50 g x L(-1) saponin under optimal condition, while the single leaching percentages of Pb were 31.56% and 28.03%, respectively. The result indicated the removal efficiency of Pb was more significant than that of Cu. After 4 times of leaching, the cumulative leaching percentages of Cu reached 58.92% and 53.11%, while the cumulative leaching percentages of Pb reached 77.69% and 65.32% for single and mixed contaminated Lou soil, respectively. The fractionation results of heavy metals in soil before and after a single leaching showed that the contents of adsorbed and exchangeable Cu and Pb increased in the contaminated soil, while the carbonate-bound, organic bound and sulfide residual Cu and Pb in the contaminated Lou soil could be effectively removed by saponin.
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April 2015

Erratum to: Arsenic Concentrations and Associated Health Risks in Laccaria Mushrooms from Yunnan (SW China).

Biol Trace Elem Res 2015 Jun;165(2):233-4

Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China.

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http://dx.doi.org/10.1007/s12011-015-0321-8DOI Listing
June 2015

Clinical significance of upregulation of mir-196a-5p in gastric cancer and enriched KEGG pathway analysis of target genes.

Asian Pac J Cancer Prev 2015 ;16(5):1781-7

Department of Clinical Testing Teaching and Research, School of Medical Technology, Gansu Traditional Chinese Medical University, Lanzhou, China E-mail :

Background: miRNAs are relatively recently discovered cancer biomarkers which have important implications for cancer early diagnosis, treatment and estimation of prognosis. Here we focussed on expression of mir-196a-5p in gastric cancer tissues and cell lines so as to analyse its significance for clinicopathologic characteristics and generate enriched KEGG pathways clustered by target genes for exploring its potential roles as a biomarker in gastric cancer.

Materials And Methods: The expression of mir-196a-5p in poorly, moderate and well differentiated gastric cancer cell lines compared with GES-1 was detected by RT-qPCR, and the expression of mir-196a-5p in gastric cancer tissues comparing with adjacent non cancer tissues of 58 cases were also assessed by RT- qPCR. Subsequently, an analysis of clinical significance of mir-196a-5p in gastric cancer and enriched KEGG pathways was executed based on the miRWalk prediction database combined with bioinformatics tools DAVID 6.7 and Mirfocus 3.0.

Results: RT-qPCR showed that mir-196a-5p was up-regulated in 6 poorly and moderate differentiated gastric cancer cell lines SGC-7901, MKN-45, MKN-28, MGC-803, BGC-823, HGC-27 compared with GES-1, but down-regulated in the highly differentiated gastric cancer cell line AGS. Clinical data indicated mir-196a-5p to beup-regulated in gastric cancer tissues (47/58). Overexpression of mir-196a-5p was associated with more extensive degree of lymph node metastasis and clinical stage (P <0.05; x2 test). Enriched KEGG pathway analyses of predicted and validated targets in miRWalk combined with DAVID 6.7 and Mirfocus 3.0 showed that the targeted genes regulated by mir-196a-5p were involved in malignancy associated biology.

Conclusions: Overexpression of mir-196a-5p is associated with lymph node metastasis and clinical stage, and enriched KEGG pathway analyses showed that targeted genes regulated by mir-196a-5p may contribute to tumorgenesis, suggesting roles as an oncogenic miRNA biomarker in gastric cancer.
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http://dx.doi.org/10.7314/apjcp.2015.16.5.1781DOI Listing
January 2016

Arsenic concentrations and associated health risks in Laccaria mushrooms from Yunnan (SW China).

Biol Trace Elem Res 2015 Apr 23;164(2):261-6. Epub 2014 Dec 23.

Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, 650200, Kunming, China.

Some species of Laccaria have been known to contain relatively high levels of arsenic in Europe and are used as edible mushrooms in the southwest China. One population of Laccaria proxima and one population of L. vinaceoavellanea as well as topsoil (0-10 cm) they grew on were collected from natural habitats of Yunnan (SW China), while other samples such as Laccaria mushroom samples without soil were purchased from four different local markets in Yunnan. Concentrations of arsenic were determined in fruit bodies of the mushrooms and in the soils by using atomic fluorescence spectrometry to assess potential health risks of these species. The mean arsenic concentrations in caps were 135, 14.1-143, 5.5 and 130-163 mg kg(-1) dry weight (dw) for Laccaria amethystina, Laccaria laccata, L. proxima and L. vinaceoavellanea, respectively. The mean value for bioconcentration factor of arsenic in caps of L. vinaceoavellanea was 29.1 for soil with arsenic content at 5.6 mg kg(-1) dw, which indicate that L. vinaceoavellanea is an accumulator for arsenic. Caps of L. amethystina, L. laccata and L. vinaceoavellanea consumed at a volume of 300 g fresh weight for a single meal in a week can yield an exposure amount of arsenic at 4.1, 0.42-4.3 and 3.9-4.9 mg, respectively. These values are higher than the limit dose for the intake of inorganic arsenic recommended by the Joint FAO/WHO Expert Committee on Food Additives.
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http://dx.doi.org/10.1007/s12011-014-0213-3DOI Listing
April 2015

Cryptococcosis in kidney transplant recipients in a Chinese university hospital and a review of published cases.

Int J Infect Dis 2014 Sep 22;26:154-61. Epub 2014 Jul 22.

Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China. Electronic address:

Background: Cryptococcosis is a severe fungal infection with a high mortality rate among solid-organ transplant recipients. Today, China is among the countries performing the most kidney transplants worldwide, however data on the association of cryptococcosis with kidney transplantation in mainland China remain scarce and fragmented.

Methods: We retrospectively analyzed cases of culture-confirmed cryptococcosis following kidney transplantation that have occurred at our hospital and reviewed the published cases in China over the last 30 years.

Results: Cryptococcosis in kidney transplant recipients was mainly caused by Cryptococcus neoformans var. grubii VNI strains and occurred most frequently in patients aged 41-50 years (37.9%, 11/29). The average time to infection after kidney transplantation was 5.16 ± 3.97 years. The clinical manifestations were found to be diverse, with slight to moderate headache and fever, meningeal irritation, and high cerebrospinal fluid pressure being relatively common. Physicians should be alert to these symptoms among kidney transplant recipients.

Conclusions: Cryptococcosis is a serious infection among kidney transplant recipients in mainland China. It has unique characteristics, such as a relatively long time to onset after kidney transplantation, and diverse clinical manifestations. Treatment with intrathecal injection of amphotericin B is considered effective for central nervous system involvement. The findings of this study also highlight the urgent need for multicenter, prospective, and multidisciplinary clinical studies and education on cryptococcosis in kidney transplant recipients in China.
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http://dx.doi.org/10.1016/j.ijid.2014.05.028DOI Listing
September 2014

[Advance in studies on effective components in wheat bran and their pharmacological activities].

Zhongguo Zhong Yao Za Zhi 2014 Jan;39(2):175-80

Wheat bran, as the testa of wheat, has a long history of medication. Modern studies have discovered that wheat bran contains dietary fiber, phenolic compounds, proteins, vitamins, minerals and many other compounds, and boasts wide pharmacological activities such as blood glucose reduction, hypertension reduction, lipid reduction, anti-oxidation, anti-bacteria, anti-inflammatory, antivirus, prevention of colon cancer and mutations, immunomodulation and adsorption of heavy metals. With great development and utilization values, wheat bran has long attracted wide attention from Chinese and foreign scholars. The paper summarizes the latest advance in domestic and foreign studies on effective components in wheat bran and their pharmacological effect, and gives a brief introduction of the limiting factors in the comprehensive development and utilization of wheat bran, in order to provide new preference for the development and utilization of abundant wheat bran resources in China.
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January 2014

Echocardiographic diagnosis of sinus of Valsalva aneurysm: a 17-year (1995-2012) experience of 212 surgically treated patients from one single medical center in China.

Int J Cardiol 2014 Apr 15;173(1):33-9. Epub 2014 Feb 15.

Department of Ultrasonography, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei Provincial Key Laboratory of Molecular Imaging, Wuhan 430022, China.

Objective: To evaluate the value of echocardiography in the diagnosis of different pathological patterns of sinus of Valsalva aneurysms (SVAs).

Methods: Echocardiographic features and surgical findings of 212 consecutive patients with SVAs treated in the last 17 years (1995-2012) at the Union Hospital of Huazhong University of Science and Technology were compared and analyzed retrospectively.

Results: 212 Chinese patients with SVAs underwent surgical repairs from January 1995 to May 2012 in our hospital. The aneurysms originated from the right, non-, multiple and left coronary sinus in 77.8%, 19.3%, 2.4% and 0.5%, respectively. 71.7% were ruptured, most commonly into the right ventricle (67.9%) followed by the right atrium (27.4%). Other rare entry sites of rupture included the left atrium, the left ventricle, the interatrial septum, the interventricular septum and the pulmonary artery (0.5%-1.9%). 164 SVAs arising from the right coronary sinus were classified by the Sakakibara method: 47.6% type I, 33.5% type II, 6.1% type IIIv and 12.8% type IIIa. 41 aneurysms of the non-coronary sinus were classified by the Guo method: 61.0% type I, 34.1% type IIa and 4.9% type IIv. The three most common associated cardiovascular lesions were ventricular septal defect (VSD) (53.3%), stenosis of right ventricular outflow tract (RVOTS) (7.5%) and aortic valvular malformations (5.2%). Compared with surgical results, the sensitivity, specificity and accuracy of echocardiographic diagnosis of SVAs were 93.9%, 99.9% and 99.8%, respectively. Of the 13 SVAs that were missed on echocardiography, 77% were small aneurysms of the right coronary sinus extending into the right ventricle across a VSD. Of the 199 cases diagnosed by echocardiography prior to surgery, the diagnostic accuracy of aneurismal origination, termination and whether ruptured or not was 99.0%, 99.0% and 97.5%, respectively. Echocardiography also diagnosed accurately all of the complications of the SVAs with the exception of aneurismal vegetations. The sensitivity, specificity and accuracy of echocardiography in diagnosing the associated cardiovascular lesions were 89.2%, 99.9% and 99.0%, respectively. The most common misdiagnosis and misdiagnosed associated lesions were the RVOTS and the types of VSD, respectively.

Conclusions: Echocardiography has a specific value in the diagnosis of different pathological patterns of the SVAs with distinguishing ultrasonic features. To the best of our knowledge, this is the largest series of patients with SVAs surgically treated in a single medical center.
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http://dx.doi.org/10.1016/j.ijcard.2014.02.003DOI Listing
April 2014

Synergetic decolorization of reactive blue 13 by zero-valent iron and anaerobic sludge.

Bioresour Technol 2013 Dec 17;149:38-43. Epub 2013 Sep 17.

Department of Chemistry, University of Science & Technology of China, Hefei 230026, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.

Efficient decolorization of reactive blue 13 was achieved by a combined use of anaerobic sludge and zero-valent iron (ZVI), and the underlying mechanism of this process was elucidated, Addition of 1.0 g/L ZVI into sludge accelerated the decolorization, with the decoloration ratio after 1-h treatment increased by 29.4% compared with the sum of the individual systems, indicating a synergy between the sludge and ZVI. Meanwhile, substantial changes in sludge morphology and microbial community were observed. The increased dye removal by ZVI was mainly attributed to: (1) a directly chemical reduction and aggregation; and (2) creation of a more favorable pH and anaerobic environment for microorganisms. The acid production by acidogens also partially offset the pH rise in ZVI corrosion and would alleviate ZVI deactivation. This work might offer valuable implications for the optimization and practical application of ZVI-anaerobic sludge processes for treatment of azo dyes or other recalcitrant pollutants.
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http://dx.doi.org/10.1016/j.biortech.2013.09.041DOI Listing
December 2013

Coronary sinus septal defect (unroofed coronary sinus): echocardiographic diagnosis and surgical treatment.

Int J Cardiol 2013 Sep 23;168(2):1258-63. Epub 2012 Dec 23.

Department of Ultrasonography, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology; Hubei Provincial Key Laboratory of Molecular Imaging, Wuhan, China.

Objective: To explore the value of transthoracic and right heart contrast echocardiography in the diagnosis of coronary sinus septal defect (CSSD), also known as unroofed coronary sinus.

Methods: The echocardiographic characteristics of 20 patients with CSSD who underwent surgery in our hospital between October 1999 and June 2012 were reviewed retrospectively, including results of 9 cases studied by contrast echocardiography, and compared with surgical results.

Results: Of the coronary sinuses in these 20 patients, 40% were totally unroofed, 35% partially unroofed in mid-portion, 20% partially unroofed in terminal portion, and 5% mixed type (mid- and terminal portions). Of these 20 patients, 65% were associated with other types of atrial septal defects, and 65% had a persistent left superior vena cava. The diagnostic accuracy of echocardiography for the CSSD was 65%. In patients whose diagnoses were confirmed by echocardiography, the accuracy for the types of CSSD was 84.6%. All of the first 6 cases studied before 2002 were either undiagnosed or misdiagnosed, and 78.6% of the last 14 cases studied were diagnosed accurately. Of 9 patients who underwent contrast echocardiography, 8 were correctly diagnosed, including 5 type II and 3 Raghib syndromes. 1 case of type IIIa was misdiagnosed as Raghib syndrome.

Conclusions: The combination of transthoracic and contrast echocardiography can diagnose and classify CSSD accurately in most cases and should be the first choice for diagnosing CSSD.
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http://dx.doi.org/10.1016/j.ijcard.2012.11.113DOI Listing
September 2013

Malakoplakia of the esophagus caused by human papillomavirus infection.

World J Gastroenterol 2012 Dec;18(45):6690-2

Department of Pathology, Yunnan Province Second People's Hospital, Kunming 650021, Yunnan Province, China.

Malakoplakia is a rare granulomatous disease probably caused by infection and characterized histologically by Michaelis-Gutmann bodies. We report a more rarely seen case esophageal malakoplakia in a 54-year-old woman. She presented with coughing while eating and drinking. Gastroscopy showed yellow nodules in the esophagus, and endoscopic ultrasonography showed a space-occupying lesion in the substratum of the esophageal mucosa. All findings highly resembled esophageal cancer. Histopathological examination finally indentified this space-occupying lesion as malakoplakia and not cancer. Immunohistochemistry showed that she had human papillomavirus (HPV) infection in the esophagus, which indicates that infection was responsible for the malakoplakia. This is believed to be the first case of malakoplakia in the esophagus, and more importantly, we established that HPV infection was the initiator of esophageal malakoplakia.
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http://dx.doi.org/10.3748/wjg.v18.i45.6690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516212PMC
December 2012

Influence of chondroitin sulfate and hyaluronic acid on structure, mechanical properties, and glioma invasion of collagen I gels.

Biomaterials 2011 Nov 5;32(31):7932-40. Epub 2011 Aug 5.

Department of Chemistry, Columbia University, New York, NY 10027, USA.

To mimic the extracellular matrix surrounding high grade gliomas, composite matrices composed of either acid-solubilized (AS) or pepsin-treated (PT) collagen and the glycosaminoglycans chondroitin sulfate (CS) and hyaluronic acid (HA) are prepared and characterized. The structure and mechanical properties of collagen/CS and collagen/HA gels are studied via confocal reflectance microscopy (CRM) and rheology. CRM reveals that CS induces fibril bundling and increased mesh size in AS collagen but not PT collagen networks. The presence of CS also induces more substantial changes in the storage and loss moduli of AS gels than of PT gels, in accordance with expectation based on network structural parameters. The presence of HA significantly reduces mesh size in AS collagen but has a smaller effect on PT collagen networks. However, both AS and PT collagen network viscoelasticity is strongly affected by the presence of HA. The effects of CS and HA on glioma invasion is then studied in collagen/GAG matrices with network structure both similar to (PT collagen-based gels) and disparate from (AS collagen-based gels) those of the corresponding pure collagen matrices. It is shown that CS inhibits and HA has no significant effect on glioma invasion in 1.0 mg/ml collagen matrices over 3 days. The inhibitory effect of CS on glioma invasion is more apparent in AS than in PT collagen gels, suggesting invasive behavior in these environments is affected by both biochemical and network morphological changes induced by GAGs. This study is among the few efforts to differentiate structural, mechanical and biochemical effects of changes to matrix composition on cell motility in 3D.
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http://dx.doi.org/10.1016/j.biomaterials.2011.07.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159748PMC
November 2011

Pore size variable type I collagen gels and their interaction with glioma cells.

Biomaterials 2010 Jul;31(21):5678-88

Department of Chemistry, Columbia University, New York, NY 10027, USA.

Gelation temperatures from 22 degrees C to 37 degrees C were used to control the pore size of collagen matrices independent of collagen concentration. To limit cell exposure to temperatures lower than physiological temperature, the putative nucleation and growth mechanism of collagen was investigated to determine the time at which gel fibril and network structure becomes independent of temperature. It was found that the temperature dependent portion of collagen gelation ends close to the time at which fibrils first form a network spanning structure. These findings were then exploited to prepare cell-embedded gels nucleated at 22, 27, or 32 degrees C and then incubated at 37 degrees C. This achieves fibrillar and network structure characteristic of gels formed solely at the nucleation temperature. Proof of principle studies of glioma invasion in these gels suggested pore size is a key determinant of glioma invasive speed in collagen gels.
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http://dx.doi.org/10.1016/j.biomaterials.2010.03.039DOI Listing
July 2010

[Determination of the components of bitespiramycin by HPLC].

Yao Xue Xue Bao 2009 Oct;44(10):1183-6

National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.

The paper is to report the establishment of an HPLC method for determination of the components of bitespiramycin and its products, and to evaluate the components profile of bitespiramycin and its related products. Liquid chromatography combined with mass spectrometry (LC-MS) was used to identify the nine major components of the reference substance of bitespiramycins. The nine components of bitespiramycins and its products have been quantified by HPLC with gradients elution methods. The content of the component of 4"-O-isovalerylspiramycin III was not less than 35%, the content of the components of 4"-O-isovalerylspiramycin (I + II + III) was not less than 60%, and the contents of the nine components of bitespiramycin were not less than 80%, separately. In addition to the components mentioned above, there also exists some other impurities, however, with lower contents. This gradient elution HPLC method reported by this paper is considered to be suitable for the quality control of bitespiramycin.
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October 2009
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