Publications by authors named "Ya-Fei Guo"

12 Publications

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Concurrent and reactivation of hepatitis B virus infection in diffuse large B-cell lymphoma: risk factors and survival outcome.

Infect Agent Cancer 2018 12;13:40. Epub 2018 Dec 12.

Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000 China.

Objective: To determine the clinical features and survival difference of HBV related and Non-HBV related diffuse large B-cell lymphoma (DLBCL) and to evaluate the occurrence of HBV reactivation in DLBCL patients and related risk factors for HBV reactivation after R-CHOP therapy.

Methods: A total of 246 patients diagnosed with CD20+ DLBCL were enrolled from June 2010 to June 2015. The medical records and survival data were analysed. Multivariate logistic regression analysis was used to identify predictors of HBV reactivation. Survival curves were performed by the Kaplan-Meier method.

Results: Among patients enrolled, 80 patients were HBsAg sero-positive and 166 patients were HBsAg sero-negative. Findings showed that HBsAg sero-negative patients were significantly older than that of patients with HBsAg sero-positive ( <  0.001). Proportion of B symptom positive patients in HBsAg sero-positive were higher ( = 0.002). Higher LDH level ( = 0.019) and late Ann Arbor stage ( = 0.010) were more often observed in patients with HBsAg sero-positive. The rate of complete response, partial response, stable disease and progress disease in HBsAg sero-negative group were 63.9, 16.9, 1.1 and 18.1%, respective, which is significantly higher than that in HBsAg sero-positive group (36.2, 18.8, 1.2 and 43.8%). Kaplan-Meier analysis showed that DLBCL patients with HBsAg sero-negative had better prognosis. In total, 17 patients showed HBV reactivation among 166 patients (10.2%) with HBsAg sero-negative after R-CHOP treatment, while a significant higher HBV reactivation 18.75% (9/48) in HBsAb negative group were observed, with 8.25% (8/97) patients in HBsAb level 10-100 U/mL group, and 0% patients in HBsAb level higher than 100 U/mL group. Multivariable analysis showed that serum HBsAb and serum HBcAb were independent risk factors for HBV reactivation in DLBCL patients.

Conclusion: Our data revealed that characteristics and prognosis were significantly different between HBV related DLBCL than non-HBV related DLBCL patients. DLBCL patients with resolved hepatitis B are at a higher risk of developing HBV reactivation after R-CHOP chemotherapy compared with HBsAg-negative/HBcAb negative patients.
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http://dx.doi.org/10.1186/s13027-018-0215-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6292058PMC
December 2018

[Effect of Chlorambucil on the Apoptotic Signaling Pathway in Lymphoma Cells].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2018 Oct;26(5):1360-1365

Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.

Objective: To study whether chlorambucil has apoptotic effect on the B cell lymphoma A20 cells and its exact mechanisms in apoptotic signaling pathway.

Methods: The experimental cells were treated with 20 μmol/L chlorambucil, the control cells were treated with PBS. Annexin V-FITC Cell Apoptosis Detection Kit was used to examine cell apoptosis. Western blot was used to detect the expressions of active caspase-3, Survivin, NF-B and pAKT. Real-time fluorescent quantitative PCR was performed to examine the mRNA expression of Survivin.

Results: Compared with the control group, the proportion of FITC/PI apoptotic cells and the expression of active caspase-3 (t=7.384, P=0.000) in the chlorambucil treatment group was significantly elevated. However, the expression of Survivin mRNA (t=4.384, P=0.000), protein expressions of survivin (t=12.360, P=0.000), NF-B (t=5.462, P=0.000) and pAKT (t=7.183, P=0.000) in the chlorambucil-treated group all significantly decreased.

Conclusion: The chlorambucil can induce the apoptosis of lymphoma cells, its mechanism may related with inhibition of PI3K/AKT signaling pathway, and expression of NF-B and survivin.
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http://dx.doi.org/10.7534/j.issn.1009-2137.2018.05.017DOI Listing
October 2018

[Clinical Analysis of EB Virus Infection Complicated with Hemophagocytic Syndrome and Hodgkin's Lymphoma].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2018 Aug;26(4):1072-1078

Department of Hematology,The Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,Fujian Province,China.

Objective: To investigate the clinical characteristics and outcome of parhents with EBV infection conbined with hemophagocytic syndrome and Hodgkin's lymphoma.

Methods: The morphotogy of bone marrow cells was observed by bone marrow smear and light microscopy, the pathologic changes of bone marrow ware analyzed by bone marrow biopsy and immunohistochemistry methord, the pathologic changes of lymphonudes ware detected by immunohistochemical methord, the paticnts were treated with ABVD (epirubicin, bleomycin, vincristine and dacarbazine) chemotherapeutic regimen.

Results: Fever complicatid with pancytopenia, obvious increase of ferritin and sCD25, hypofibrinogenemia, hemophogocytic phenomen of bone marrow, increase of EBV-DNA copy number ware observed, which all accorded with the criteria EBV righted hemophagocytic syndrome. The curative efficacy of amtiinfective treatmatnt was poor, After treatment with HLH-2004 regimen, the fever symptome and the laboratory indicaters such as whole blood cells, ferritin and fibrinogen all were recovered to normal levels. Left mandibular lymphadenctasis was confirmed as Hodgkin's lymphoma (mixed cell type) by pathological examination. The patient achieved complete molecular remission after 1 course chemotherapy with ABVD regimen. The level of EBV-DNA copy number were also decreased. As the reshlt, the patient's hemophagocytic syndrome had bean effectively controlled, and the Hodgkin's lymphoma is still in complete remission.

Conclusion: Epstein-Barr virus-ratated hemophagocytic syndrome and Hodgkin's lymphoma are rare, and their long-term prognosis needs to be further explored.
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http://dx.doi.org/10.7534/j.issn.1009-2137.2018.04.022DOI Listing
August 2018

[Clinical Characteristics and Prognosis of patients with Variant Philadelphia Chromosome Positive Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2018 Aug;26(4):1056-1061

Department of Hematology,The Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,Fujian Province,China.

Objective: To study the clinical characteristics and prognosis of patients with variant Ph chromosome-positive leukemia.

Methods: The defection of morphology, cytogenetics, immunology and molecular biology was performed in 4 cares of variant Ph chromosome-positive leukemia, and the therepeuitics outcome of 4 patients was evaluated.

Results: Among 4 cases of variant Ph+ leukemia, 3 cases were patients with CML, including 1 case in chronic phase and 2 cases in accelerated phase; and 1 cases was patient with adult B acute lymphoblasric leukemia(B-ALL).The defecfion of cytogenetics in 4 cases showed that 2 cases of CML displayed t(9; 22; 14) abnormality, 1 case of CML displayed t(5; 9; 22) abnormality, moreover, the BCR/ABL fution gane in 3 cases of CML all was e14a2 type, 1 cases of adult B-ALL disylayed t(9; 22; 17) abnormatlity, BCR/ABL fution gene of this case was e13a3 type, 4 patients all received treatment wire chemotherapeptic regimen contaiming methanesulfanate imatinib. As a result, 1 cases of adult B-ALL with e13a3 type BCR/ABL fusion gene positive relapsed after molecular biology remission for 4 months and died in the 10th month; and yet 3 cases of CML are still in molecular biology remission, the disease-free survival time of these 3 cases was 10, 19 and 27 months respectively.

Conclusion: The patients with variant Ph chromosome-positive leukemia will response to the first generation tyrosine kinase inhibitors, but the prognosis of patients with e13a3 type of BCR/ABL fusion gene remains to be further explored.
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http://dx.doi.org/10.7534/j.issn.1009-2137.2018.04.019DOI Listing
August 2018

Inclusion complexes of GA and the plant growth regulation activities.

Mater Sci Eng C Mater Biol Appl 2018 Oct 18;91:475-485. Epub 2018 May 18.

Key Laboratory of Medicinal Chemistry for Natural Resources, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, PR China. Electronic address:

Gibberellic acid (GA) is an important phytohormone that is applied in agriculture, nurseries, tissue culture, tea gardens, etc. However, it has some drawbacks such as potential hazardous effects on mammals and labile in the condition of a weak base or acid. In this study, the enhanced stability and bioavailability of GA were achieved by forming the inclusion complexes of GA with cyclodextrins (β- or γ-CD) and its derivative (HP-β-CD). In the preliminary plant growth regulation assay, GA/CDs displays superior bioactivity compared to pure GA to help with the early seedling growth of cucumber and mung bean and the root growth of cucumber and mung bean, respectively. The results showed that there was a certain relationship between the inclusion ability, stability and bioactivity. The inclusion stability constants of gibberellin clathrate are consistent with the order of stabilities of the inclusion complex. Among these complexes, GA/HP-β-CD possess highest inclusion constant, and the binding ability of the HP-β-CD not only enhances the stability of gibberellic acid in the stability test but also plays a slow release role in the bioactivity assay. Therefore, the complex of GA may be used as a promising plant growth regulator.
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http://dx.doi.org/10.1016/j.msec.2018.05.043DOI Listing
October 2018

Oxidation of Aromatic Aldehydes to Esters: A Sulfate Radical Redox System.

J Org Chem 2017 02 23;82(3):1591-1599. Epub 2017 Jan 23.

Beijing Key Laboratory of Ionic Liquids Clean Process, Key Laboratory of Green Process and Engineering, State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences , Beijing 100190, PR China.

A mild oxidative esterification of various aromatic aldehydes by sulfate radical redox system was presented. In the reaction pathway exploration, the transiency of MeOSO was disclosed, which was generated from esterification between the in situ generated HSO and MeOH, a rate-limiting step in the process. More importantly, the selectivity-controlling step was represented by the subsequent nucleophilic displacement between MeOSO and aldehydes. The ionic oxidant 1a ((NH)SO) with more N-H numbers in the cation, as compared with 1c ((n-BuN)SO) and 1d ((PyH)SO), has better performance in the oxidative esterification of aldehydes.
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http://dx.doi.org/10.1021/acs.joc.6b02775DOI Listing
February 2017

Hepatocyte growth factor promotes proliferation, invasion, and metastasis of myeloid leukemia cells through PI3K-AKT and MAPK/ERK signaling pathway.

Am J Transl Res 2016 15;8(9):3630-3644. Epub 2016 Sep 15.

Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital Fuzhou, China.

This study aims to investigate effects of HGF expression on biological behaviors of Kasumi-1 and HL60. Expression of HGF and c-Met gene were detected using qRT-PCR. Short hairpin RNA (shRNA) was used to reduce HGF expression. Silencing effect of shRNA was verified by qRT-PCR and western blot. Cell reproductive capacity, cell clonality and cell cycle (apoptosis) were detected by CCK-8, clone formation, flow cytometry (FCM), respectively. Cell adhesion, cell invasion ability and cell proliferation were also examined. Changes of PI3K-AKT, MAPK/ERK signaling factors were detected by western blot. HGF and c-Met expression in first-vist AML group was significantly higher than in AML-relief and normal control group. HGF shRNA can inhibit cell proliferation, inhibit cloning ability. Compared with control group, apoptosis ratios of Kasumi-1 and HL60 cell in interference groups were significantly higher. After shRNA interference, the number of adherent cells and transmembrane cells were significantly decreased compared with control group. Meanwhile, shRNA also down-regulated Bad, Bcl-XL, Bcl-2, CDK1, Cyclin B, MMP2, MMP9, and up-regulated cleaved caspase9, cleaved caspase3, cleaved PARP, Bax, and P21. Moreover, phosphorylated c-Met, AKT, Erk, and mTOR were also reduced. In conclusion, HGF and c-Met gene highly expressed among first-visit AML patients, but decreased after relief treatment. HGF may promote proliferation, invasion, and metastasis of AML cells through PI3K-AKT and MAPK/ERK signaling pathway. Therefore, proliferation and invasion ability of AML cell can be inhibited by down-regulating HGF gene to retardate cell in G2/M stage.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040664PMC
September 2016

[Expression of HIF-1α in primary acute myeloid leukemia cells and its relationship with prognosis].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2015 Feb;23(1):19-23

Fujian Insitute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China. E-mail:

Objective: This study was aimed to detect the expression of HIF-1α in acute myeloid leukemia (AML) except acute promyelocyte leukemia (APL) and investigate the relationship of its expression levels with clinical parameters and prognosis.

Methods: The primary AML cells were collected from peripheral blood of 53 newly diagnosed AML patients by using CD3 negative sorting. The expression of HIF-1α was measured by real-time fluorescent quantitative PCR (FQ-PCR) , and the relationship between expression level of HIF-1α and clinical parameters (age, sex, WBC count, clinical typing, prognosis) was analysed according to relative expression level. Furthermore, Western blot was used to detect the protein level of HIF-1α in AML patients with or without extramedullary infiltration.

Results: The expression level of HIF-1α did not correlate with age, sex, WBC count, Hb level, Plt count and the percentage of blast. There was no significant difference of HIF-1α expression between different AML subtype based on FAB. The higher level of HIF-1α was found in AML patients who did not get complete remission after one or two courses of chemotherapy, however, the difference was not statistically significant. The relapse rate was higher in AML patients with the higher expression of HIF-1α. In addition, the higher level of HIF-1α mRNA and protein were found in bone marrow of AML patients with extramedullary infiltration (P < 0.01). The negative correlation between HIF-1α and PTEN was observed (r = -0.48, P = 0.001).

Conclusions: Overexpression of HIF-1α are closely related with extramedullary infiltration and prognosis of acute myeloid leukemia, and may be used as an early indicator of extramedullary infiltration and prognosis.
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http://dx.doi.org/10.7534/j.issn.1009-2137.2015.01.004DOI Listing
February 2015

[A new method of anti-jamming ability improvement for Michelson Interferometer].

Guang Pu Xue Yu Guang Pu Fen Xi 2014 May;34(5):1425-8

In order to improve anti-jamming capability of Michelson interferometer system, replace the traditional structure of the moving mirror scanning was replaced, an interference system based on electro-optic modulation of crystal refractive index was designed to achieve optical path scanning. The system modulated voltage signal on the variable refractive crystal, to generate cyclical changes, changed the refractive index to control optical path difference in the original optical path system. Using electronic scanning to replace of mechanical scanning, improved the system's noise immunity was improved. In the electro-optic modulation process, computed the maximum optical path difference of the system was computed, and analyzed of the crystal thickness and crystal diffraction efficiency of the modulation process were analyzed. The simulation experiment shows that, with the modulation voltage range increasing, the available range of the optical path is also increased, and the system spectrum resolving power will also increase accordingly. Meanwhile, in the modulation process set the modulation range was set to make the energy of diffraction energy losses less than 10% of the total energy, so as to ensure a better signal to noise ratio. Experimental results show that, as the modulation voltage changes, interference fringes occurred continuously moved. When the voltage is further increased, the nonlinear error appears. After non-linear error correction for the system, spectrum resolution reached to 7. 2 cm-1, slightly lower than the original system. But its anti-jamming capability is greatly enhanced, as in the absence of experimental platform for seismic conditions, conventional interferometer relative error is more than 20%, while the relative error of the system is less than 5%, in line with the design requirements. It was proved that the anti-jamming capability of the system was enhanced greatly, when the static electro-optical modulation was used.
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May 2014

[Prediction of heat-related mortality impacts under climate change scenarios in Shanghai].

Zhonghua Yu Fang Yi Xue Za Zhi 2012 Nov;46(11):1025-9

School of Public Health, Peking Union Medical College, Beijing 100730, China.

Objective: To project the future impacts of climate change on heat-related mortality in shanghai.

Methods: The statistical downscaling techniques were applied to simulate the daily mean temperatures of Shanghai in the middle and farther future under the changing climate. Based on the published exposure-reaction relationship of temperature and mortality in Shanghai, we projected the heat-related mortality in the middle and farther future under the circumstance of high speed increase of carbon e mission (A2) and low speed increase of carbon emission (B2). The data of 1961 to 1990 was used to establish the model, and the data of 1991 - 2001 was used to testify the model, and then the daily mean temperature from 2030 to 2059 and from 2070 to 2099 were simulated and the heat-related mortality was projected. The data resources were from U.S. National Climatic Data Center (NCDC), U.S. National Centers for Environmental Prediction Reanalysis Data in SDSM Website and UK Hadley Centre Coupled Model Data in SDSM Website.

Results: The explained variance and the standard error of the established model was separately 98.1% and 1.24°C. The R(2) value of the simulated trend line equaled to 0.978 in Shanghai, as testified by the model. Therefore, the temperature prediction model simulated daily mean temperatures well. Under A2 scenario, the daily mean temperature in 2030 - 2059 and 2070 - 2099 were projected to be 17.9°C and 20.4°C, respectively, increasing by 1.1°C and 3.6°C when compared to baseline period (16.8°C). Under B2 scenario, the daily mean temperature in 2030 - 2059 and 2070 - 2099 were projected to be 17.8°C and 19.1°C, respectively, increasing by 1.0°C and 2.3°C when compared to baseline period (16.8°C). Under A2 scenario, annual average heat-related mortality were projected to be 516 cases and 1191 cases in 2030 - 2059 and 2070 - 2099, respectively, increasing 53.6% and 254.5% when compared with baseline period (336 cases). Under B2 scenario, annual average heat-related mortality were projected to be 498 cases and 832 cases in 2030 - 2059 and 2070 - 2099, respectively, increasing 48.2% and 147.6% when compared with baseline period (336 cases).

Conclusion: Under the changing climate, heat-related mortality is projected to increase in the future;and the increase will be more obvious in year 2070 - 2099 than in year 2030 - 2059.
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November 2012

Assessing heat-related mortality risks in Beijing, China.

Biomed Environ Sci 2012 Aug;25(4):458-64

Institute of Environmental Health and Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: To obtain the exposure-response relationship for temperature and mortality, and assess the risk of heat-related premature death.

Methods: A statistical model was developed using a Poisson generalized linear regression model with Beijing mortality and temperature data from October 1st, 2006 to September 30th, 2008. We calculated the exposure-response relationship for temperature and mortality in the central city, and inner suburban and outer suburban regions. Based on this relationship, a health risk model was used to assess the risk of heat-related premature death in the summer (June to August) of 2009.

Results: The population in the outer suburbs had the highest temperature-related mortality risk. People in the central city had a mid-range risk, while people in the inner suburbs had the lowest risk. Risk assessment predicted that the number of heat-related premature deaths in the summer of 2009 was 1581. The city areas of Chaoyang and Haidian districts had the highest number of premature deaths. The number of premature deaths in the southern areas of Beijing (Fangshan, Fengtai, Daxing, and Tongzhou districts) was in the mid-range.

Conclusion: Ambient temperature significantly affects human mortality in Beijing. People in the city and outer suburban area have a higher temperature-related mortality risk than people in the inner suburban area. This may be explained by a temperature-related vulnerability.
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http://dx.doi.org/10.3967/0895-3988.2012.04.011DOI Listing
August 2012

[Clinical comparative study on the influence of acupoint sticking therapy in dog days and in non-dog days to the quality of life of allergic rhinitis patients].

Zhongguo Zhen Jiu 2012 Jan;32(1):31-4

School of TCM, Southern Medical University, Guangzhou 510515, Guangdong Province, China.

Objective: To observe and compare the influence of acupoint sticking therapy in dog days and in non-dog days to the quality of life of allergic rhinitis patients.

Methods: Fifty-five cases were divided into group A (the acupoint sticking therapy in dog days group, 28 cases) and group B (the acupoint sticking therapy in non-dog day group, 27 cases) randomly. The acupoint sticking therapy description (Dazhui (GV 14), Fengmen (BL 12), Feishu (BL 13) etc. was used in both groups. Five times of acupoint sticking therapy were given to patients in group A during dog days in 2010, while another 5 times of acupoint sticking therapy were given to patients in group B before the dog days. The Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ) was used to evaluate the effects before, after and half a year after treatment.

Results: The scores of behavior problems, nasal symptoms and quality of life in RQLQ of both groups all decreased after treatment (all P < 0.01). The score of emotion reaction of group A in the follow-up period decreased compared with that before treatment (P < 0.05). The scores of non-nasal-or-ocular symptoms, ocular symptoms and emotion reaction after treatment and in the follow-up period all decreased than those before treatment (P < 0.01, P < 0.05). The decreasing degrees of scores of non-nasal-or-ocular symptoms, ocular symptoms, emotion reaction and quality of life after treatment and in the follow-up period in group A were all greater than those in group B (P < 0.01, P < 0.05).

Conclusion: In the same season, both acupoint sticking therapy in dog days and in non-dog dags can improve the symptoms of allergic rhinitis, but the improving degree to quality of life in the method of acupoint sticking in dog days is greater.
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January 2012