Publications by authors named "Ya Nan Wang"

337 Publications

Preparation of high solid content oxidized starch by acid pretreatment-HO oxidation and its performance as the ligand in zirconium tanning.

Carbohydr Res 2022 Jan 13;511:108501. Epub 2022 Jan 13.

National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu, 610065, China; Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu, 610065, China.

Highly-oxidized starch (HOS) is an excellent ligand for zirconium tanning in leather making. However, oxidation reaction can only be undertaken in diluted solution due to the high viscosity of starch, which leads to high oxidant consumption and low solid content of HOS, and therefore greatly limits its industrial application. Starch was predegraded by using HCl followed by HO oxidation for preparing a practical oxidized starch (OST) ligand with high solid content. HCl (5%) remarkably reduced the viscosity of starch and simplified the subsequent oxidation, resulting in a decrease in HO dosage from 60% to 40% and an increase in solid content from 10% to 38%. HCl broke the α-(1-4) glycosidic bond between glucose units, and HO oxidation led to the generation of abundant carboxyl groups in OST. The OST with moderate molecular weight (Mw 5609 g/mol) and carboxyl content (6.48 mmol/g) made the tanning performance of OST-Zr comparable to those of HOS-Zr and chrome tanning agents. Therefore, this work gives new insights into the crop-polysaccharides based ligands application and exhibits huge potential for promoting industrial applications of zirconium tanning.
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http://dx.doi.org/10.1016/j.carres.2022.108501DOI Listing
January 2022

Minor terpenoids from the stems and twigs of .

J Asian Nat Prod Res 2022 Jan 18:1-12. Epub 2022 Jan 18.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

Four minor undescribed terpenoids, including a monoterpenoid () and three triterpenoids (, and ), together with 26 known terpenoids were isolated from the stems and twigs of . Their structures were identified by extensive spectroscopic analyses and electronic circular dichroism (ECD) techniques. Compound showed excellent cytotoxicity against human colon cancer cell (HCT-116) with IC value of 2.56 μM. Compounds and exhibited partly inhibitory effects on nitric oxide production stimulated by lipopolysaccharide-induced neuroinflammation in microglia cells at 10 μM with inhibition ratios of 39.70% and 28.08%, respectively.
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http://dx.doi.org/10.1080/10286020.2022.2027916DOI Listing
January 2022

Remediation of Cr(VI)-contaminated soil by combined chemical reduction and microbial stabilization: The role of biogas solid residue (BSR).

Ecotoxicol Environ Saf 2022 Jan 13;231:113198. Epub 2022 Jan 13.

School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao, China.

In this work, the use of chemical reduction combined with microbial stabilization to remediate Cr(VI) in contaminated soil was systematically investigated. The effectiveness, phytotoxicity and microbial diversity resulting from the combination of ferrous sulfate with microbial stabilization by biogas solid residue (BSR) were determined. The stabilization experiments showed that the optimum Cr(VI) conversion rate of 99.92% was achieved with an Fe (II)/Cr(VI) molar ratio of 3:1, a BSR dose of 5.2% (wt), and a water content of 40%. Under these conditions, the residual Cr(VI) content was 0.80 mg/kg, which satisfied the risk screening value (≤ 5.7 mg/kg) for soil contamination of land for general development in China. The remaining Cr(VI) level was stable for 90 days during the chemical reduction and biogenic stabilization process. Moreover, Zucconi test analysis suggested that the soil phytotoxicity to Brassica campestris L. disappeared. The results of microbial diversity analysis indicated that the bacterial community changed significantly during chemical reduction and microbial stabilization processes, and Bacillus, Pseudomonas and Psychrobacter may participate in the reduction of Cr(VI) into Cr(III).
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http://dx.doi.org/10.1016/j.ecoenv.2022.113198DOI Listing
January 2022

and Nor- Isodhilarane-Type Meroterpenoids from and the Configuration Revisions of Related Compounds.

J Nat Prod 2022 Jan 3. Epub 2022 Jan 3.

Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China.

and nor- isodhilarane-type meroterpenoids (SIMs and NSIMs) are mainly found in fungi and have been characterized by highly congested polycyclic skeletons and a broad range of bioactivities. However, the literature reports inconsistent configuration assignments for some SIMs and NSIMs, due to their complex polycyclic systems and multichiral centers. Herein, we described eight SIMs and NSIMs isolated from the EtOAc extract of , which led to the configuration revisions of purpurogenolide C (), berkeleyacetal B (), chrysogenolide F (), and berkeleyacetal C () as compounds -, respectively. Furthermore, extensive re-evaluation of the experimental and computational C NMR chemical shifts of the reported 39 SIMs and NSIMs provided an empirical approach for determining the C-9 relative configuration, according to the C NMR chemical shifts of C-9, which contributed to the configuration revisions of another three SIMs ( and ) and NSIMs (), denoted as compounds -, respectively. Biological assays indicated that compound exhibited cytotoxic activity against HepG2 and A549 cell lines with IC values of 5.58 and 6.80 μM, respectively. Compounds -, , , and showed moderate hepatoprotective activity at 10 μM in the APAP-induced HepG2 cell injury model.
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http://dx.doi.org/10.1021/acs.jnatprod.1c01025DOI Listing
January 2022

Effect of type 2 diabetes mellitus on mandibular bone regeneration and the expression of T helper cell 17/regulat-ory T cell-related factors in mice.

Hua Xi Kou Qiang Yi Xue Za Zhi 2021 Dec;39(6):642-650

Dept. of Implantology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan 250012, China.

Objectives: To observe the effect of type 2 diabetes mellitus (T2DM) on mandibular bone regeneration and the expression of factors related to T helper cell 17 (Th17 cell) and regulatory T cell (Treg cell) in mice.

Methods: Thirty-six 6-week-old C57BL/6J male mice were randomly divided into normal control (NC) and T2DM groups. Fasting blood glucose levels were detected 0 d, 7 d, 14 d, and 28 d after surgery for mandibular defects. Hematoxylin-eosin (HE) staining was used in observing the bone after 7 d, 14 d, and 28 d of the healing process. Immunohistochemical staining was used in observing the expression of alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), forkhead box protein P3 (Foxp3), retinoic acid related orphan receptor gamma T (RORγt), and protein tyrosine phosphatase non-receptor type 2 (PTPN2) after 7 d, 14 d, and 28 d of healing.

Results: HE staining showed that the area with new bones in the T2DM group was significantly smaller than that in the NC group. Immunohistochemical staining showed that the expression of osteogenesis related proteins ALP and RUNX2 were significantly reduced in the T2DM group. In addition, the number of RORγt positive cells increased, whereas the number of Foxp3 positive cells and the expression PTPN2 decreased significantly in the mandibular bone defect in mice with T2DM.

Conclusions: T2DM significantly inhibit mandibular bone regeneration in mice. Decline in PTPN2 expression and the transition of Treg and Th17 may be the underlying molecular mechanisms.
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http://dx.doi.org/10.7518/hxkq.2021.06.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703102PMC
December 2021

Two new diterpenoids from the stems of as GABA receptor agonists.

J Asian Nat Prod Res 2021 Dec 2:1-8. Epub 2021 Dec 2.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.

Two new diterpenoids, dauricumins A () and B (), together with two known aromatic meroterpenoids ( and ), were isolated from the petroleum ether soluble fraction of the stems from through an HPLC-MS-SPE-NMR combination strategy. The absolute configurations of and were elucidated by ECD calculations and [Rh (OCOCF)]-induced CD spectrum analysis. In a membrane potential FLIPR assay, confluentin () showed an agonistic effect on GABA receptor (EC = 20 µM).
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http://dx.doi.org/10.1080/10286020.2021.2007089DOI Listing
December 2021

13,13a-seco-protoberberines from the tubers of Corydalis yanhusuo and their anti-inflammatory activity.

Phytochemistry 2022 Feb 25;194:113023. Epub 2021 Nov 25.

Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, China. Electronic address:

Six undescribed protoberberine derivatives including two pairs of enantiomers, named yanhusanines G-L, along with fifteen reported protoberberine alkaloids, were isolated from the tubers of Corydalis yanhusuo. Among them, yanhusanines H-L feature a unique 13,13a-seco skeleton which is rare in nature. Their structural elucidations were achieved by extensive spectroscopic analysis and quantum chemistry calculations. A biogenetic route for yanhusanines H-L was proposed. Bioassay results showed that yanhusanine J exhibited potent inhibitory effect against the nitric oxide (NO) production in lipopolysaccharide (LPS) induced RAW 264.7 cells (IC = 2.25 ± 1.32 μM). Western blot analysis demonstrated that yanhusanine J exerted its anti-inflammatory effect via suppressing the nuclear factor kappa B (NF-κB) pathway, together with the decrease of the inflammatory factors TNF-α, IL-6 and IL-1β. Furthermore, molecular simulation docking indicated that yanhusanine J had strong interaction with the active site of the inducible nitric oxide synthase (iNOS) protein.
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http://dx.doi.org/10.1016/j.phytochem.2021.113023DOI Listing
February 2022

Establishment of reference (housekeeping) genes via quantitative real-time PCR for investigation of the genomic basis of abiotic stress resistance in Psammochloa villosa (Poaceae).

J Plant Physiol 2022 Jan 20;268:153575. Epub 2021 Nov 20.

School of Life Sciences, Qinghai Normal University, Xining, 810008, China.

Psammochloa villosa is a desert plant growing in Northwest China with considerable resistance to abiotic stress, including drought, cold, and salt. To facilitate future studies of stress resistance in Psammochloa villosa, we sought to establish a suite of reference (or housekeeping) genes for utilization within future gene expression studies. Specifically, we selected nine candidate genes based on prior studies and new transcriptomic data for P. villosa, and we evaluated their expression stability in three different tissues of P. villosa under different treatments simulating abiotic stress conditions using four different bioinformatics assessments. Our results showed that TIP41 (TIP41-like family protein) was the most stable reference gene in drought- and salt-stressed leaves and salt-stressed stems, ELF-1α (elongation factor 1-α) was the most stable in cold-stressed leaves and drought- and salt-stressed roots, ACT (actin) was the most stable in drought-stressed stems, TUA (α-tubulin) was the most stable in cold-stressed stems, and 18S rRNA (18S ribosomal RNA) was the most stable in cold-stressed roots. Additionally, we tested the utility of these candidate reference genes to detect the expression pattern of P5CS (Δ-pyrroline-5-carboxylate synthetase), which is a drought-related gene. This study is the first report on selecting and validating reference genes of P. villosa under various stress conditions and will benefit future investigations of the genomic mechanisms of stress resistance in this ecologically important species.
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http://dx.doi.org/10.1016/j.jplph.2021.153575DOI Listing
January 2022

Polyhydroxylated bergamotane-type sesquiterpenoids from cultures of Paraconiothyrium sporulosum YK-03 and their absolute configurations.

Phytochemistry 2022 Feb 15;194:113000. Epub 2021 Nov 15.

Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China.

Eight undescribed polyhydroxylated bergamotane-type sesquiterpenoids with bicyclic, tricyclic and tetracyclic systems, namely sporulamides A-D, sporulosoic acids A-B and sporuloketals A-B, along with three known analogs were isolated from cultures of the marine mud-associated fungus Paraconiothyrium sporulosum YK-03. The chemical structures of these sesquiterpenoids were elucidated by the extensive spectroscopic techniques of NMR and HR-ESI-MS. Assisted by the X-ray crystallography analysis and electronic circular dichroism (ECD) spectroscopic calculation and comparison, their absolute configurations were established. Sporuloketals A-B represent two rare tetracyclic bergamotanes. It's the first time that ECD empirical rules have been successfully verified and applied for determining the absolute configurations of these bergamotane-type sesquiterpenoids.
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http://dx.doi.org/10.1016/j.phytochem.2021.113000DOI Listing
February 2022

Piezoelectric Effect and Ignition Characteristics of Coal Mine Gob Roof Collapse.

ACS Omega 2021 Nov 21;6(43):28936-28945. Epub 2021 Oct 21.

School of Resource, Environment and Safety Engineering, Hunan University of Science and Technology, Xiangtan 411201, Hunan, China.

Existing studies of coal self-ignition and impact frictional sparks do not provide valid support for the analysis of ignition sources in all cases of methane explosions in the gob. In this paper, the explosion in the gob of the Renlou coal mine is used to investigate the piezoelectric effect and ignition characteristics of roof collapse in identifying a new ignition source of gas explosion. Experimental and theoretical analyses conclude that the piezoelectric effect is produced by quartz, which is the main constituent of the roof sandstone. During the loading process, the piezoelectric effect and compressive strength are key factors in the gathering of free charges on rock tips. During rupture, the rock tip retains a large number of charges, forming a ″point-surface″ effect, which triggers an electron avalanche accompanied by an orange-yellow spark lasting over 22 ms, far exceeding the ionization energy and ignition induction period of methane-air mixtures. The piezoelectric effect and compressive strength of the rock cause the generation of electrical sparks, which is the ignition source of the explosion in the gob of Renlou mine II722.
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http://dx.doi.org/10.1021/acsomega.1c03987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567394PMC
November 2021

Role of epigenetic modifications in the development of crops essential traits.

Yi Chuan 2021 Sep;43(9):858-879

College of Life Sciences, Northeast Forestry University, Harbin 150040, China.

Epigenetic modification refers to the chemical modifications of chromosomal DNA and histones, mainly including DNA methylation, histone modifications and non-coding RNAs. Without altering the DNA sequence, these heritable modifications can affect gene expression profiles by changing the chromatin state and play an important role in regulating the growth and development of plants. When the specific epigenetic modifications are changed, crops can obtain excellent phenotypes and stronger environmental adaptability. Therefore, artificially changing the epigenetic modifications are expected to obtain high-quality germplasm resources more suitable for agricultural production. In this review, we summarize the main types of plant epigenetic modifications, highlight the research progresses of functional plant epigenetic modifications on the important traits and responses to environmental stress, and identify the main problems that need be solved in the application of epigenetics in crop improvement, thereby providing new insights for the functional epigenetic modifications on crop breeding and improvement.
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http://dx.doi.org/10.16288/j.yczz.21-170DOI Listing
September 2021

Characterization of the complete chloroplast genome of (Typhaceae) from Jilin Province, China and phylogenetic analysis.

Mitochondrial DNA B Resour 2021 20;6(11):3253-3254. Epub 2021 Oct 20.

Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

The complete chloroplast genome of was sequenced and assembled in this study. The circular genome is 160,391 bp in length and exhibits a typical quadripartite structure with a large single-copy (LSC, 87,660 bp) and small single-copy (SSC, 18,721 bp) regions, separated by a pair of inverted repeats (IRs, 27,005 bp). The cp genome contains 113 unique genes, including 79 protein-coding, 30 tRNA, and four rRNA genes. The phylogenetic analysis within the Poales showed that is monophyletic and most closely related to . Within , is sister to the clade of and . The work reported here will provide useful information for the evolutionary studies on the genus of .
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http://dx.doi.org/10.1080/23802359.2021.1991247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8530496PMC
October 2021

Bioactive sesquineolignans from the twigs of Litsea cubeba.

Chin J Nat Med 2021 Oct;19(10):796-800

Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China; State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address:

In a continuing search for biological natural products with structure diversity from traditional Chinese herbs, five new sesquineolignans (1-5) were isolated from an ethyl acetate extract of the twigs of Litsea cubeba. Their structures were elucidated based on MS, 1D and 2D NMR spectroscopic data, as well as experimental electronic circular dichroism (ECD) spectra. Compounds 1-5 showed moderate inhibitory effects against LPS-induced NO production in RAW264.7 macrophages, with IC values of 16.2, 20.2, 22.1, 15.1, and 16.6 μmol·L, respectively.
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http://dx.doi.org/10.1016/S1875-5364(21)60075-4DOI Listing
October 2021

[Seasonal Variation and Influencing Factor Analysis of Antibiotic Resistance Genes in Water Supply Reservoirs of Central China].

Huan Jing Ke Xue 2021 Oct;42(10):4753-4760

Henan Key Laboratory for Synergistic Prevention of Water and Soil Environmental Pollution, School of Geographic Sciences, Xinyang Normal University, Xinyang 464000, China.

This study quantified an integron gene and 19 antibiotic resistance genes(ARGs) to identify the ARGs pollution characteristics in 11 drinking water reservoirs of central China. The results indicated that the ARGs abundance did not change significantly over time in the studied reservoir waterbodies. Tetracycline, sulfonamide, and -lactam ARGs were dominant. The high abundance and detection rate of two sulfonamide ARGs( and ) suggested that they were the predominant ARGs. No polymyxin resistance genes() were detected, which indicated that the antibiotic restriction policy of China has achieved positive outcomes. Compared with that in other environmental media, the ARGs abundance in the reservoir environment was low. The correlation analysis showed relevance between the water quality indicators and the ARGs, which suggested that the water quality indexes can be used as ARGs pollution indicators in the reservoir environment. The abundance and detection rate of carbapenem ARGs were low owing to their dosage restriction and high degradability. Tetracycline ARGs were closely related to the other resistance gene types, which might have been due to horizontal gene transfer. Although the overall correlation between and ARGs was modest, it might be the main reason for the spread of several individual ARGs in the reservoir environment.
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http://dx.doi.org/10.13227/j.hjkx.202102127DOI Listing
October 2021

Cadmium and arsenic availability in soil under submerged incubation: The influence of humic substances on iron speciation.

Ecotoxicol Environ Saf 2021 Dec 13;225:112773. Epub 2021 Sep 13.

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-environment, Ministry of Agriculture, Beijing 100081, China; College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China. Electronic address:

Humic substances (HSs), as electron shuttles, are associated with iron oxide transformation, yet the manner by which HSs affect Cd/As availabilities during this process under anaerobic conditions remains unclear. Two HSs (humic sodium, HA-Na, and biochemical fulvic acid, BFA) were applied at 0, 1, 2, and 4 gCkg in a submerged incubation experiment. The dissolved, extractable and fractions of Cd/As and different iron oxides in soils were monitored. The addition of both HA-Na and BFA decreased the CaCl-extractable Cd by 12.66-93.13%, and increased the KHPO-extractable As by 18.81-71.38% on the 60th day of incubation. The soil Eh and crystalline iron oxides (Fed) decreased, while amorphous iron oxides (Feo) and dissolved As increased after addition of both HSs. However, the two HSs had opposite effects on soil pH and dissolved Cd at the end of the incubation. HA-Na immobilized 19.47-85.99% more available Cd than did BFA over the incubation, although the extent of immobilization was similar with the maximum application rate on the 60th day. BFA mobilized 5.22-26.12% more available As than did HA-Na. XPS data showed that FeOOH decreased while the FeO component increased over the incubation. Correlation analysis and SEM showed that the reduction in the soil Eh and Fed and relative increase in Feo increased the available Cd, while decreased the available As. Consequently, the addition of HA-Na and BFA, particularly combined with flooding irrigation management, could effectively reduce the available Cd in Cd-contaminated soil. However, this method should be used with caution in As-contaminated soil.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112773DOI Listing
December 2021

Neuronal architecture and functional mapping of the taste center of larval Helicoverpa armigera (Lepidoptera: Noctuidae).

Insect Sci 2021 Aug 24. Epub 2021 Aug 24.

Department of Entomology, College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, China.

The sense of taste plays a crucial role in herbivorous insects by discriminating nutrients from complex plant metabolic compounds. The peripheral coding of taste has been thoroughly studied in many insect species, but the central gustatory pathways are poorly described. In the present study, we characterized single neurons in the gnathal ganglion of Helicoverpa armigera larvae using the intracellular recording/staining technique. We identified different types of neurons, including sensory neurons, interneurons, and motor neurons. The morphologies of these neurons were largely diverse and their arborizations seemingly covered the whole gnathal ganglion. The representation of the single neurons responding to the relevant stimuli of sweet and bitter cues showed no distinct patterns in the gnathal ganglion. We postulate that taste signals may be processed in a manner consistent with the principle of population coding in the gnathal ganglion of H. armigera larvae.
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http://dx.doi.org/10.1111/1744-7917.12965DOI Listing
August 2021

Chemical-activity-based quality marker screening strategy for Viscum articulatum.

Biomed Chromatogr 2021 Dec 8;35(12):e5175. Epub 2021 Sep 8.

Academician Workstation, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.

Viscum articulatum Burm. f. is a parasitic plant rich in flavonoids, triterpenoids, and catechins and has a high nutritional value. It has been reported that consuming V. articulatum can prevent cardiac diseases. In this study, six bioactive compounds, including catechins, triterpenoids, and phenylpropanoid glycosides, were determined in alcohol extracts of the plant using HPLC. The anti-inflammatory and antioxidant activities of three catechins, two triterpenoids, and three combination drugs were measured in cardiomyocytes, and the results showed that the anti-inflammatory activity was significantly enhanced while retaining strong antioxidant activity when epicatechin and ursolic acid were used in combination. The main quality markers epicatechin and ursolic acid were screened based on the specificity of the genuine herb and a potent synergistic effect, and the lowest limitation contents of V. articulatum which could discriminate it from some other taxonomically similar materials were accordingly determined. This self-built lowest limitation content of the two screened quality markers could quickly and accurately reflect the efficacy in terms of chemical composition and reverse the disorderly market use of nongenuine herbs or confusing species for adulteration. This study is of some significance for market regulation, drug development, and clinical medication.
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http://dx.doi.org/10.1002/bmc.5175DOI Listing
December 2021

Protein Tyrosine Kinase 7 Regulates EGFR/Akt Signaling Pathway and Correlates With Malignant Progression in Triple-Negative Breast Cancer.

Front Oncol 2021 22;11:699889. Epub 2021 Jul 22.

Institute of Life Science and Green Development, Hebei University, Baoding, China.

Purpose: Triple-negative breast cancer (TNBC), the most aggressive subtype of breast cancer, is associated with high invasiveness, high metastatic occurrence and poor prognosis. Protein tyrosine kinase 7 (PTK7) plays an important role in multiple cancers. However, the role of PTK7 in TNBC has not been well addressed. This study was performed to evaluate the role of PTK7 in the progression of TNBC.

Methods: Correlation of PTK7 expression with clinicopathological parameters was assessed using tissue microarray immunohistochemistry (IHC) staining in 280 patients with breast cancer. PTK7 expression in TNBC (MDA-MB-468, MDA-MB-436 and MDA-MB-231) and non-TNBC (MCF7 and SK-BR-3) breast cancer cell lines were examined using immunoblotting assay. PTK7 correlated genes in invasive breast carcinoma were analyzed using cBioPortal breast cancer datasets including 1,904 patients. PTK7 overexpressed or knockdown TNBC cell lines (MDA-MB-468 and MDA-MB-436) were used to analyze the potential roles of PTK7 in TNBC metastasis and tumor progression. A TNBC tumor bearing mouse model was established to further analyze the role of PTK7 in TNBC tumorigenicity .

Results: PTK7 is highly expressed in breast cancer and correlates with worse prognosis and associates with tumor metastasis and progression in TNBC. Co-expression analysis and gain- or loss-of-function of PTK7 in TNBC cell lines revealed that PTK7 participates in EGFR/Akt signaling regulation and associated with extracellular matrix organization and migration genes in breast cancer, including COL1A1, FN1, WNT5B, MMP11, MMP14 and SDC1. Gain- or loss-of-function experiments of PTK7 suggested that PTK7 promotes proliferation and migration in TNBC cell lines. PTK7 knockdown MDA-MB-468 cell bearing mouse model further demonstrated that PTK7-deficiency inhibits TNBC tumor progression .

Conclusion: This study identified PTK7 as a potential marker of worse prognosis in TNBC and revealed PTK7 promotes TNBC metastasis and progression EGFR/Akt signaling pathway.
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http://dx.doi.org/10.3389/fonc.2021.699889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339706PMC
July 2021

Gingipain-Responsive Thermosensitive Hydrogel Loaded with SDF-1 Facilitates Periodontal Tissue Regeneration.

ACS Appl Mater Interfaces 2021 Aug 29;13(31):36880-36893. Epub 2021 Jul 29.

Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong 250012, China.

Existing local drug delivery systems for periodontitis suffer from poor antibacterial effect and unsatisfied periodontal regeneration. In this study, a smart gingipain-responsive hydrogel ([email protected]) was synthesized as an environmentally sensitive carrier for on-demand drug delivery. The [email protected] hydrogel was synthesized from polyethylene glycol diacrylate (PEG-DA) based scaffolds, dithiothreitol (DTT), and a novel designed functional peptide module (FPM) via Michael-type addition reaction, and the hydrogel was further loaded with stromal cell derived factor-1 (SDF-1). The FPM exhibiting a structure of anchor peptide-short antimicrobial peptide (SAMP)-anchor peptide could be cleaved by gingipain specifically, and the SAMP was released out of the hydrogel for antibacterial effect in response to gingipain. The hydrogel properties were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), swelling ratio analysis, degradation evaluation, and release curve description of the SAMP and SDF-1. Results indicated the [email protected] hydrogel exhibited preferable biocompatibility and could promote the proliferation, migration, and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Antibacterial testing demonstrated that the [email protected] hydrogel released the SAMP stressfully in response to gingipain stimulation, thereby strongly inhibiting the growth of . Furthermore, the study indicated that the [email protected] hydrogel inhibited reproduction, created a low-inflammatory environment, facilitated the recruitment of CD90+/CD34- stromal cells, and induced osteogenesis. Taken together, these results suggest that the gingipain-responsive [email protected] hydrogel could facilitate periodontal tissue regeneration and is a promising candidate for the on-demand local drug delivery system for periodontitis.
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http://dx.doi.org/10.1021/acsami.1c08855DOI Listing
August 2021

Cytotoxicity and Apoptosis Induced by L. Essential Oil in Human Normal Liver Cell Line L02 via the Endogenous Mitochondrial Pathway Rather Than the Endoplasmic Reticulum Stress.

Int J Environ Res Public Health 2021 07 13;18(14). Epub 2021 Jul 13.

West China School of Public Health, Sichuan University, Chengdu 610017, China.

L. ( has been used as dietary condiments and as traditional medicine in South America. The oil of L. ( can be used as a natural antioxidant in food processing. It also has analgesic, sedating, and deworming effects, and can be used along with the whole plant for its medical effects: decongestion, as an insecticide, and to offer menstruation pain relief. This study was conducted to investigate the cytotoxicity and apoptosis effects of an essential oil from in vitro. The cytotoxicity evaluation of the essential oil from on human normal liver cell line L02 was assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. AO/EB dual fluorescent staining assay and Annexin V-FITC were used for apoptosis analysis. The changes in mitochondrial membrane potential (MMP) were analyzed with 5,5,6,6'-tetrachloro-1,1,3,3,-tetraethyl-imidacarbocyanine iodide (JC-1) dye under a fluorescence microscope. The level of apoptosis related protein expression was quantified by Western blot. The L02 cells were treated with the essential oil from at 24, 48, and 72 h, and the IC values were 65.45, 58.03, and 35.47 μg/mL, respectively. The AO/EB staining showed that viable apoptotic cells, non-viable apoptotic cells, and non-viable non-apoptotic cells appeared among the L02 cells under the fluorescence microscope. Cell cycle arrest at the S phase and cell apoptosis increased through flow cytometry in the L02 cells treated with the essential oil. MMP decreased in a concentration-dependent manner, as seen through JC-1 staining under the fluorescence microscope. In the L02 cells as shown by Western blot and qPCR, the amount of the apoptosis-related proteins and the mRNA expression levels of cytochrome C, Bax, Caspase-9, and Caspase-3 increased, Bcl-2 decreased, and Caspase-12, which is expressed in the endoplasmic reticulum, showed no obvious changes in protein amount or mRNA expression level. The essential oil form had a cytotoxic effect on L02 cells. It could inhibit L02 cell proliferation, arrest the cell cycle at the S phase, and induce L02 cell apoptosis through the endogenous mitochondrial pathway.
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http://dx.doi.org/10.3390/ijerph18147469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304090PMC
July 2021

T Cell Protein Tyrosine Phosphatase in Glucose Metabolism.

Front Cell Dev Biol 2021 29;9:682947. Epub 2021 Jun 29.

Department of Implantology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, China.

T cell protein tyrosine phosphatase (TCPTP), a vital regulator in glucose metabolism, inflammatory responses, and tumor processes, is increasingly considered a promising target for disease treatments and illness control. This review discusses the structure, substrates and main biological functions of TCPTP, as well as its regulatory effect in glucose metabolism, as an attempt to be referenced for formulating treatment strategies of metabolic disorders. Given the complicated regulation functions in different tissues and organs of TCPTP, the development of drugs inhibiting TCPTP with a higher specificity and a better biocompatibility is recognized as a promising therapeutic strategy for diabetes or obesity. Besides, treatments targeting TCPTP in a specific tissue or organ are suggested to be considerably promising.
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http://dx.doi.org/10.3389/fcell.2021.682947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276021PMC
June 2021

[Revealing the mechanisms underlying the therapeutic effects of acupuncture on migraine using neuroimaging: a narrative review].

Sheng Li Xue Bao 2021 Jun;73(3):433-445

Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.

Migraine is a neurological disorder characterized by attacks of moderate or severe headache and various neurological symptoms. Acupuncture, as a commonly used non-pharmacological therapy, has the advantage of obvious therapeutic effect and few side effects in the prevention and treatment of migraine. But the underlying mechanism of acupuncture on migraine remains unclear. Recently, advances in neuroimaging technology have helped to objectively assess the effect of acupuncture on treating migraine and offered new opportunities to explore the central mechanism of acupuncture on treating migraine. In order to better understand the current status of neuroimaging studies on the therapeutic mechanism of acupuncture on migraine and shed light on future research, this review aims to overview the neuroimaging studies in recent 10 years from two aspects: (1) Central mechanism of acupuncture on treating acute migraine attack; (2) Central mechanism of acupuncture on preventing migraine attack.
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June 2021

Pharmic Activation of PKG2 Alleviates Diabetes-Induced Osteoblast Dysfunction by Suppressing PLC1-Ca-Mediated Endoplasmic Reticulum Stress.

Oxid Med Cell Longev 2021 16;2021:5552530. Epub 2021 Jun 16.

Department of Implantology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China.

As reported in our previous study, cinaciguat can improve implant osseointegration in type 2 diabetes mellitus (T2DM) rats by reactivating type 2 cGMP-dependent protein kinase (PKG2), but the downstream mechanisms remain unclear. In the present study, we investigated the favorable effect of cinaciguat on primary rat osteoblast, which was cultivated on titanium disc under vitro T2DM conditions (25 mM glucose and 200 M palmitate), and clarified the therapeutic mechanism by proteomic analysis. The results demonstrated that T2DM medium caused significant downregulation of PKG2 and induced obvious osteoblast dysfunction. And overexpression of PKG2 by lentivirus and cinaciguat could promote cell proliferation, adhesion, and differentiation, leading to decreased osteoblasts injury. Besides, proteomic analysis revealed the interaction between PKG2 and phospholipase C1 (PLC1) in the cinaciguat addition group, and we further verified that upregulated PKG2 by cinaciguat could inhibit the activation of PLC1, then relieve intracellular calcium overload, and suppress endoplasmic reticulum (ER) stress to ameliorate osteoblast functions under T2DM condition. Collectively, these findings provided the first detailed mechanisms responsible for cinaciguat provided a favorable effect on promoting osseointegration in T2DM and demonstrated a new insight that diabetes mellitus-induced the aberrations in PKG2-PLC1-Ca-ER stress pathway was one underlying mechanism for poor osseointegration.
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http://dx.doi.org/10.1155/2021/5552530DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225424PMC
December 2021

Viscosity sensitive endoplasmic reticulum fluorescent probes based on oxazolopyridinium.

J Mater Chem B 2021 07;9(28):5664-5669

College of Chemistry, Chemical Engineering and Material Science, Soochow University, 199 Ren'Ai Road, Suzhou 215123, China.

A series of viscosity sensitive fluorescent probes 1a-e were synthesized by linking coumarin and oxazolopyridinium via dimethylene in this paper. The viscosity test of probes 1a-e indicated that the fluorescence intensity of the probes enhanced significantly with the increase of viscosity of the system (0.89-865 cP), and exhibited a nearly OFF-ON response to viscosity at 648 nm, 650 nm and 650 nm, respectively. In addition, cells still had a high survival rate after co-culturing with probes 1a-e for 12 h (94-98%). Meanwhile, the laser confocal experiment showed that the variation of the carbon chain length in the oxazolopyridinium could affect the subcellular region of the localization of the probes in cells. When the length of the carbon chain in oxazolopyridinium was between n-C7H15 and n-C12H23, probes 1b-d had the ability to target the endoplasmic reticulum in the cells. Moreover, probes 1b-d showed no significant change in fluorescence intensity after 35 min of continuous laser confocal irradiation, indicating that they had excellent anti-photobleaching properties.
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http://dx.doi.org/10.1039/d1tb01106eDOI Listing
July 2021

Characterization of HPV subtypes not covered by the nine-valent vaccine in patients with CIN 2-3 and cervical squamous cell carcinoma.

Curr Probl Cancer 2021 12 9;45(6):100761. Epub 2021 Jun 9.

Department of Gynecology and Obstetrics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China. Electronic address:

Background: As the second most common female malignant tumor, cervical cancer is also one of the most preventable and avoidable cancers. The World Health Organization has launched a global plan to accelerate the elimination of cervical cancer. Therefore, in the era of postvaccine, the role of HPV subtypes in cervical precancerous lesions and cervical cancer that are not covered by vaccine should be further discussed. The purpose of this study was to explore the role of HPV subtypes not covered by the nine-valent vaccine in high-grade cervical precancerous lesions and cervical cancer.

Materials And Methods: A retrospective analysis was performed on the clinical data of 5220 patients with an HPV infection who were diagnosed and treated in the Department of Gynecology of Shanghai General Hospital between October 2016 and February 2020. In addition, the clinical characteristics of the biopsy results of 470 cases of cervical intraepithelial neoplasia (CIN) 2-3 and 205 cases of cervical squamous cell carcinoma were analyzed.

Results: Among patients with HPV subtype infection not covered by the nine-valent vaccine, univariate analysis showed that compared with patients with CIN 2-3, age ≥ 50, not using condom and TCT reported as ASC-H were risk factors for cervical squamous cell carcinoma (P < 0.05). The detection rates of HPV subtype not covered by the nine-valent vaccine in CIN 2-3 and cervical squamous cell carcinoma patients were 7.23% and 6.34%, respectively.

Conclusion: In patients with CIN 2-3 and cervical squamous cell carcinoma, the infection rates of HPV subtype not covered by the nine-valent vaccine were 7.23% and 6.34%, respectively. With the increasing popularity of the vaccine, the infection rates of the corresponding HPV subtype decreased; however, HPV subtype infection not covered by the nine-valent vaccine should not be ignored.
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http://dx.doi.org/10.1016/j.currproblcancer.2021.100761DOI Listing
December 2021

Online discovery of the molecular mechanism for directionally detoxification of Fuzi using real-time extractive electrospray ionization mass spectrometry.

J Ethnopharmacol 2021 Sep 24;277:114216. Epub 2021 May 24.

State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, PR China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, PR China. Electronic address:

Ethnopharmacological Relevance: Aconitum carmichaelii Debeaux, a famous traditional medicinal herb for collapse, rheumatic fever, and painful joints, always raises global concerns about its fatal toxicity from toxic alkaloids when improperly processed. Therefore, it is urgent to clarify the internal molecular mechanism of processing detoxification on Aconitum and develop simple and reliable approaches for clinical application, which is also of great significance to the rational medicinal use of Aconitum.

Aim Of The Study: The study aimed at developing a complete molecular mechanism exploration strategy in complex medicinal herb decocting system, clarifying the internal molecular mechanism of processing detoxification on Aconitum, and exploring valid approaches for detoxification.

Materials And Methods: Aconiti Lateralis Radix Praeparata (Fuzi) was selected as the model for exploring the complex Aconitum detoxification mechanism using an advanced online real-time platform based on extractive electrospray ionization mass spectrometry. The methods realized the sensitive capture of dynamic trace intermediates, accurate qualitative and quantitative analysis, and real-time and long-term monitoring of multi-components with satisfactory accuracy and resistance to complex matrices.

Results: Components in the complex Aconitum decocting system were real-timely characterized and fat meat was discovered and verified to directionally detoxify Aconitum while reserving the therapy effect. More importantly, the dynamic detoxification mechanism in the chemically complex Aconitum decoction was molecularly profiled. A novel reaction pathway based on nucleophilic substitution reaction mechanism was proposed. As confirmed by the theoretic calculations at DFT B3LYP/6-31G (d) levels, fatty acids (e.g., palmitic acid) acted as a green, cheap, and high-performance catalyst and promote the decomposition of toxic diester alkaloids to non-toxic and active benzoyl-monoester alkaloids through the discovered mechanism.

Conclusion: The study exposed a novel detoxification molecular mechanism of Aconitum and provided an effective method for the safe use of Aconitum, which could effectively guide the development of traditional processing technology and compatibility regulation of the toxic herb and had great value to the modernization and standardization development of traditional medicine.
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http://dx.doi.org/10.1016/j.jep.2021.114216DOI Listing
September 2021

Nomogram Based on Neutrophil-to-Lymphocyte Ratio and Platelet-to-Lymphocyte Ratio to Predict Recurrence in Patients with Hepatocellular Carcinoma after Radiofrequency Ablation.

Cardiovasc Intervent Radiol 2021 Oct 25;44(10):1551-1560. Epub 2021 May 25.

Department of Interventional Therapy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

Objectives: To investigate the prognostic value of pre-procedure neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) and construct a nomogram to predict disease-free survival (DFS) in patients receiving radiofrequency ablation (RFA) for hepatocellular carcinoma (HCC) within Milan criteria.

Methods: The data of 515 patients of HCC within Milan criteria receiving RFA were retrospectively collected. The patients were divided into two groups: the training group (n = 382) and the validation group (n = 133). Several preprocedural variables were analyzed in the two groups to determine the prognostic factors.

Results: The median DFS time of the training and validation group was 28.4 months and 24.5 months, respectively. Multivariate analyses showed that number of lesions, alpha-feto protein levels, NLR and PLR were independent risk factors of DFS. According to the time-dependent receiver operating characteristic curve (t-ROC), the optimal cutoff value of the NLR and PLR was 1.55 and 75.30, respectively, with sensitivity of 0.737 and 0.648 and specificity of 0.541 and 0.508, respectively. The area under curve (AUC) of the t-ROC curves for the NLR was 0.662 and PLR was 0.597. The DFS was significantly higher in the NLR ≤ 1.55 group compared to NLR > 1.55 group and the PLR ≤ 75.30 group compared to PLR > 75.30 group in both training and validation datasets. Nomogram was developed based on the prognostic factors indicated by the Cox regression to predict 1-, 2-, 3- and 5-year DFS probabilities.

Conclusions: The cutoff value of the NLR and PLR was 1.55 and 75.30. This new nomogram based on NLR and PLR may provide good and individualized prediction of recurrence for HCC patients within Milan criteria after RFA.
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http://dx.doi.org/10.1007/s00270-021-02872-8DOI Listing
October 2021

Single VHH-directed BCMA CAR-T cells cause remission of relapsed/refractory multiple myeloma.

Leukemia 2021 10 24;35(10):3002-3006. Epub 2021 May 24.

Department of Hematology, Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital, Zhengzhou, 450008, Henan, China.

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http://dx.doi.org/10.1038/s41375-021-01269-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478646PMC
October 2021

Effective removal of refractory organic contaminants from reverse osmosis concentrated leachate using PFS-nZVI/PMS/O process.

Waste Manag 2021 Jun 6;128:55-63. Epub 2021 May 6.

Qingdao Xiaojianxi Leachate Treatment Co. Ltd., Qingdao, China; Qingdao Jieyuan Environment Co. Ltd., Qingdao, China.

Reverse osmosis concentrated leachate (ROCL) from landfill leachate treatment contains high amounts of refractory organics. In this study, a combination of polymerized ferric sulfate (PFS) and nanoscale zero-valent iron/peroxymonosulfate/ozone (nZVI/PMS/O) approach was adopted to remove refractory pollutants in ROCL. The effects of coagulant species, dosage and initial pH on the pre-treatment of organics from ROCL during coagulation process were investigated. Moreover, the influences of experimental factors, including initial pH, ozone doses, PMS, and nZVI on the removal of refractory organics in ROCL from coagulation effluent were systematically studied. The characteristics of organics were determined by using microscopic, spectroscopic and electron paramagnetic resonance (EPR) analyses. The batch experimental results indicated that the refractory organic contaminants in ROCL were effectively removed through PFS-nZVI/PMS/O treatment. The maximum removal efficiencies of COD and TOC were 89.1% and 83.2% under the optimum conditions: PFS of 8 g/L, ozone dose of 100 mg/min, PMS dose of 1.5 mM and nZVI dose of 10 mM, and at these conditions, the biodegradability index (BOD/COD) was enhanced from 0.02 to 0.32. The excitation-emission matrix fluorescence spectroscopy (EEM) analysis indicated that humic-like and fulvic-like substances in ROCL were effectively removed. According to EPR analysis, hydroxyl and sulfate radicals were the dominant reactive species for the degradation of organics in nZVI/PMS/O system. Overall, the environmental and economic analysis suggested that the PFS-nZVI/PMS/O system was a cost-effective method for cleaning refractory organics from ROCL.
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http://dx.doi.org/10.1016/j.wasman.2021.04.051DOI Listing
June 2021

The Influence of Lower Temperature Induction of on the Infection of Apple Trees.

Plant Dis 2021 Oct 29;105(10):2776-2780. Epub 2021 Oct 29.

College of Plant Protection, Hebei Agricultural University, Baoding 071000, China.

Apple valsa canker (AVC), caused by , is one of the most important diseases of apple trees in China. AVC occurred severely along with cold winter or cold spring. However, the effect of lower temperature on is poorly understood. This study evaluated the influence of lower temperature pretreatment of on the infection of apple twigs and leaves. The results showed that exposing to lower temperatures (between -10°C and 10°C) for more than 18 h significantly increased the disease severity of apple leaves and twigs, with a higher lesion area ratio (LAR), lesion length, and disease incidence () than that at 25°C. In addition, cold treatment ranging from -5°C to 10°C promoted colony growth. Meanwhile, the relative expression of four cell wall degrading enzyme (CWDE)-related genes pretreated at -5°C and 5°C were significantly higher than that at 25°C. The results indicated that the virulence of mycelium is sensitive to lower temperatures. After sensing lower temperature changes, can adjust its infection of apple trees by regulating the expression of pathogenicity gene and growth rate. Spring has very frequent temperature changes, and is highly invasive in this season. Therefore, more attention should be paid in spring to protecting apple trees from infection of , by reducing pruning wound formation in spring and applying protective agents to pruning wounds in time.
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http://dx.doi.org/10.1094/PDIS-01-21-0086-SCDOI Listing
October 2021
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