Publications by authors named "Ya Liu"

782 Publications

Expression of miR-155 in Serum Exosomes in Children with Epilepsy and Its Diagnostic Value.

Dis Markers 2022 26;2022:7979500. Epub 2022 Jul 26.

Department of Paediatric Rehabilitation, Linyi People's Hospital, Linyi, Shandong Province, China.

Objective: This study was designed to analyze the expression of miR-155 in serum exosomes in children with epilepsy and to explore its diagnostic value.

Methods: From March 2020 to March 2021, 43 hospitalized children with epilepsy admitted to the Department of Neurology of the hospital were included, and another 43 gender- and age-matched healthy children were randomly selected as the healthy control group during the same period. Then fasting serum samples of the two groups were collected to extract the exosomes. The morphology of the exosomes was evaluated under a transmission electron microscope, and the expression of specific protein markers on the surface was detected by Western Blot. In addition, the relative expression of miR-155 in serum exosomes in children with epilepsy with different courses of the disease and different degrees of abnormal electroencephalography (EEG) was compared, and the area under the receiver operating characteristic (ROC) curve (ROC-AUC) was used to evaluate the diagnostic value of miR-155.

Results: A higher relative expression level of miR-155 in serum exosomes was obtained in the epilepsy group, as compared to the healthy control group (P<0.05), and the relative expression of miR-155 in serum exosomes in children with epilepsy was correlated with the course of the disease and the degree of abnormal EEG (both P<0.05). In addition, the expression of miR-155 in serum exosomes showed high diagnostic efficiency for epilepsy (AUC = 0.813, P<0.05).

Conclusion: The expression of miR-155 in serum exosomes in children with epilepsy is up-regulated, and its level is related to the course of the disease and the degree of abnormal EEG, so miR-155 in serum exosomes may be used as a biomarker for the diagnosis and assessment of the severity of epilepsy.
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http://dx.doi.org/10.1155/2022/7979500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345723PMC
August 2022

Pathophysiology and transcriptomic analysis of inoculated by bark beetle-vectored fungus .

Front Plant Sci 2022 19;13:944336. Epub 2022 Jul 19.

Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, China.

Ophiostomatoid fungi exhibit a complex relationship with bark beetles; exhausting of host tree defenses is traditionally regarded as one of the key benefits provided to beetle vectors. is one of the dominant species of the mycobiota associated with genus bark beetles which infect the spruce trees across the Eurasian continent. Host spruce trees resist fungal invasion through structural and inducible defenses, but the underlying mechanisms at the molecular level, particularly with respect to the interaction between bark beetle-associated fungi and host trees, remain unclear. The aim of this study was to observe the pathological physiology and molecular changes in seedlings after artificial inoculation with strains (TS, BH, QH, MX, and LWQ). This study showed that was a weakly virulent pathogen of spruce, and that the virulent of the five strains showed differentiation. All strains could induce monoterpenoid release. A positive correlation between fungal virulence and release of monoterpenoids was observed. Furthermore, the release rate of monoterpenoids peaked at 4 days post-inoculation (dpi) and then decreased from 4 to 90 dpi. Transcriptomic analysis at 4 dpi showed that many plant-pathogen interaction processes and mitogen-activated protein kinase (MAPK) metabolic processes were activated. The expression of monoterpenoid precursor synthesis genes and diterpenoid synthesis genes was upregulated, indicating that gene expression regulated the release rate of monoterpenoids at 4 dpi. The enriched pathways may reveal the immune response mechanism of spruce to ophiostomatoid fungi. The dominant possibly induces the host defense rather than defense depletion, which is likely the pattern conducted by the pioneers of beetle-associated mycobiota, such as spp.. Overall, these results facilitate a better understanding of the interaction mechanism between the dominant association of beetles and the host at the molecular level.
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http://dx.doi.org/10.3389/fpls.2022.944336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345248PMC
July 2022

Control over emergence and alignment of transient blisters in thermo-responsive gels using hierarchically patterned substrates.

Soft Matter 2022 Aug 4. Epub 2022 Aug 4.

Chemical and Petroleum Engineering Department, University of Pittsburgh, PA, USA.

Surfaces with tunable microscale textures are vital in a large variety of technological applications, including heat transfer, antifouling and adhesion. To facilitate such broad-scale use, there is a need to create surfaces that undergo reconfigurable changes in topology and thus, enable switchable functionality. To date, there is a relative dearth of methods for engineering surfaces that can be actuated to change topography over a range of length scales, and hence, form tunable hierarchically structured layers. Combining modeling and experiments, we design a geometrically patterned, thermo-responsive poly (-isopropylacrylamide) gel film that undergoes controllable hierarchical changes in topology with changes in temperature. At the bottom, the film is covalently bound to a solid, curved substrate; at the top, the film encompasses longitudinal rectangular ridges that are oriented perpendicular to the underlying cylindrical curves. At temperatures below lower critical solution temperature (LCST), the swollen gel exhibits 3D variations in polymer density and thickness defined by the gel's top and bottom topography. As the temperature rises above LCST, the interplay between the upper ridges and lower curves in the gel drives non-uniform, directional solvent transport, the nucleation and propagation of a phase-separated higher-density skin layer, and the resulting pressure buildup within the film. These different, interacting kinetic processes lead to an instability, which produces transient microscopic blisters in the film. Through simulations, we show how tuning the width of the ridges modifies the propagation of a skin layer and creates localized pressure build-up points, which enables control over the emergence, distribution, and alignment of the microscopic blisters. Additionally, we provide a simple argument to predict the size of such microscopic features. Experiments confirm our predictions and further highlight how our computational model enables the rational design of topographical transitions in these tunable films. The development of actuatable, hierarchically structured films provides new routes for achieving switchable functionality in actuators, drug release systems and adhesives.
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http://dx.doi.org/10.1039/d2sm00579dDOI Listing
August 2022

, Encoding a Typical CC-NBS-LRR Protein, Confers Resistance to Southern Corn Rust in Maize.

Front Plant Sci 2022 12;13:951318. Epub 2022 Jul 12.

Maize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing, China.

Southern corn rust (SCR) caused by Underw. poses a major threat to maize production worldwide. The utilization of host SCR-resistance genes and the cultivation of resistant cultivars are the most effective, economical strategies for controlling SCR. Here, we identified and cloned a new SCR resistance gene, , from the elite maize inbred line Jing2416K. was found to encode a typical CC-NBS-LRR protein localized in both the nucleus and cytoplasm. This gene was constitutively expressed at all developmental stages and in all tissues examined, with the strongest expression detected in leaves at the mature stage. A transcriptome analysis provided further evidence that multiple defense systems were initiated in Jing2416K, including pathogen-associated molecular pattern-triggered immunity and effector-triggered immunity, reinforcement of cell walls, accumulation of antimicrobial compounds, and activation of phytohormone signaling pathways. Finally, we developed functional Kompetitive allele-specific PCR markers for using two conserved SNP sites and successfully applied these functional markers for the detection of and the cultivation of resistant maize cultivars, demonstrating their great potential utility in maize breeding.
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http://dx.doi.org/10.3389/fpls.2022.951318DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317930PMC
July 2022

[The Role and Function of Clinical Research Nurses in Anti-tumor Drug Clinical Trials for Lung Cancer Patients].

Zhongguo Fei Ai Za Zhi 2022 Jul;25(7):501-505

Department of Respiratory and Critical Care Medicine, Peking Union Medical College Hospital, 
Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100010, China.

Clinical trials of anti-tumor drugs is not only the important way to develop new drugs, but also the most advanced treatment methods for malignant tumors, bringing survival benefits to patients. There are a large number of new anti-tumor drug clinical trials for lung cancer patients, covering a wide variety of anti-tumor drugs, and with rapid progress and high efficiency of clinical transformation. These trials could not be carried out successfully without the joint efforts of the research team, in which the research nurses also played a role that should not be underestimated. Combined with the work content of clinical research nurses, this paper introduced the post management, role function, core competence and career development prospect of clinical research nurses in the process of carrying out clinical trial of lung cancer drugs in detail. In order to provide reference for more medical institutions to carry out related work, and promote the further development of clinical research nurses to standardization and specialization.
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http://dx.doi.org/10.3779/j.issn.1009-3419.2022.102.30DOI Listing
July 2022

Phylogenomic analysis of Salmonella Indiana ST17, an emerging MDR clonal group in China.

J Antimicrob Chemother 2022 Jul 26. Epub 2022 Jul 26.

National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, Guangdong, P. R.  China.

Objectives: To reconstruct the genomic epidemiology and evolution of MDR Salmonella Indiana in China.

Methods: A total of 108 Salmonella Indiana strains were collected from humans and livestock in China. All isolates were subjected to WGS and antimicrobial susceptibility testing. Phylogenetic relationships and evolutionary analyses were conducted using WGS data from this study and the NCBI database.

Results: Almost all 108 Salmonella Indiana strains displayed the MDR phenotype. Importantly, 84 isolates possessed concurrent resistance to ciprofloxacin and cefotaxime. WGS analysis revealed that class 1 integrons on the chromosome and IncHI2 plasmids were the key vectors responsible for multiple antibiotic resistance gene (ARG) [including ESBL and plasmid-mediated quinolone resistance (PMQR) genes] transmission among Salmonella Indiana. The 108 Salmonella Indiana dataset displayed a relatively large core genome and ST17 was the predominant ST. Moreover, the global ST17 Salmonella Indiana strains could be divided into five distinct lineages, each of which was significantly associated with a geographical distribution. Genomic analysis revealed multiple antimicrobial resistance determinants and QRDR mutations in Chinese lineages, which almost did not occur in other global lineages. Using molecular clock analysis, we hypothesized that ST17 isolates have existed since 1956 and underwent a major population expansion from the 1980s to the 2000s and the genetic diversity started to decrease around 2011, probably due to geographical barriers, antimicrobial selective pressure and MDR, favouring the establishment of this prevalent multiple antibiotic-resistant lineage and local epidemics.

Conclusions: This study revealed that adaptation to antimicrobial pressure was possibly pivotal in the recent evolutionary trajectory for the clonal spread of ST17 Salmonella Indiana in China.
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http://dx.doi.org/10.1093/jac/dkac243DOI Listing
July 2022

Ultraviolet Photodetector Based on Poly(3,4-Ethylenedioxyselenophene)/ZnO Core-Shell Nanorods p-n Heterojunction.

Nanoscale Res Lett 2022 Jul 25;17(1):67. Epub 2022 Jul 25.

State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources, College of Chemistry, Xinjiang University, Urumqi, 830017, Xinjiang, People's Republic of China.

In this work, we successfully assembled an organic-inorganic core-shell hybrid p-n heterojunction ultraviolet photodetector by the electropolymerization deposition of poly(3,4-ethylenedioxyselenophene) (PEDOS) on the surface of zinc oxide nanoarrays (ZnO NRs). The structures of composite were confirmed by FTIR, UV-Vis, XRD and XPS. Mott-Schottky analysis was used to study the p-n heterojunction structure. The photodetection properties of ZnO NRs/PEDOS heterojunction ultraviolet photodetector were systematically investigated current-voltage (I-V) and current-time (I-t) analysis under different bias voltages. The results showed that PEDOS films uniformly grew on ZnO NRs surface and core-shell structure was formed. The p-n heterojunction structure was formed with strong built-in electric field between ZnO NRs and PEDOS. Under the irradiation of UV light, the device showed a good rectification behavior. The responsivity, detection rate and the external quantum efficiency of the ultraviolet photodetector reached to 247.7 A/W, 3.41 × 10 Jones and 84,000% at 2 V bias, respectively. The rise time (τ) and fall time (τ) of ZnO NRs/PEDOS UV photodetector were obviously shortened compared to ZnO UV photodetector. The results show that the introduction of PEDOS effectively improves the performance of the UV photodetector.
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http://dx.doi.org/10.1186/s11671-022-03705-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314489PMC
July 2022

Chinese Herbal Medicine for Type 2 Diabetes Mellitus With Nonalcoholic Fatty Liver Disease: A Systematic Review and Meta-Analysis.

Front Pharmacol 2022 27;13:863839. Epub 2022 Jun 27.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

To evaluate the efficacy and safety of Chinese herbal medicine (CHM) for type 2 diabetes mellitus (T2DM) with nonalcoholic fatty liver disease (NAFLD) with current evidence. This study was registered in PROSPERO as CRD42021271488. A literature search was conducted in eight electronic databases from inception to December 2021. The primary outcomes were lipid indices and liver functions, including triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine transaminase (ALT), and aspartate transaminase (AST). Review Manager 5.2 and Stata v14.0 were applied for analysis. The research enrolled 18 RCTs with 1,463 participants. Results showed CHM combined with western medicine (WM) was more effective than WM alone in TG (weighted mean differences (WMD) = -0.35.95% confidence interval (CI) [-0.51, -0.19], < 0.0001), TC (WMD = -0.58.95%CI [-0.80, -0.36], < 0.00001), LDL-C (WMD = -0.37, 95%CI [-0.47, -0.26], < 0.00001), HDL-C (WMD = 0.20, 95%CI [0.10, 0.29], < 0.0001), ALT (WMD = -4.99, 95%CI [-6.64, -3.33], < 0.00001), AST (WMD = -4.76, 95%CI [-6.35, -3.16], < 0.00001), homeostatic model assessment of insulin resistance (WMD = -1.01, 95%CI [-1.22, -0.79], < 0.00001), fasting blood glucose (WMD = -0.87, 95%CI [-1.13, -0.61], < 0.00001), 2-h postprandial glucose (WMD = -1.45.95%CI [-2.00, -0.91], < 0.00001), body mass index (WMD = -0.73.95%CI [-1.35, -0.12], = 0.02), and overall effective rate (risk ratio (RR) = 1.37.95%CI [1.29, 1.46], < 0.00001). The CHM in combination with WM seems to be more beneficial in T2DM with NAFLD patients in improving lipid and glucose metabolism, liver function, and insulin resistance as well as improving overall efficiency and reducing body weight. Given the poor quality of reports from these studies and uncertain evidence, these findings should be interpreted cautiously. https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021271488, identifier CRD42021271488.
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http://dx.doi.org/10.3389/fphar.2022.863839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271569PMC
June 2022

Quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats.

BMC Genomics 2022 Jul 13;23(1):507. Epub 2022 Jul 13.

Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China.

Background: Changes in the abundance of ovarian proteins play a key role in the regulation of reproduction. However, to date, no studies have investigated such changes in pubescent goats. Herein we applied isobaric tags for relative and absolute quantitation (iTRAQ) and liquid chromatography-tandem mass spectrometry to analyze the expression levels of ovarian proteins in pre-pubertal (n = 3) and pubertal (n = 3) goats.

Results: Overall, 7,550 proteins were recognized; 301 (176 up- and 125 downregulated) were identified as differentially abundant proteins (DAPs). Five DAPs were randomly selected for expression level validation by Western blotting; the results of Western blotting and iTRAQ analysis were consistent. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that DAPs were enriched in olfactory transduction, glutathione metabolism, and calcium signaling pathways. Besides, gene ontology functional enrichment analysis revealed that several DAPs enriched in biological processes were associated with cellular process, biological regulation, metabolic process, and response to stimulus. Protein-protein interaction network showed that proteins interacting with CDK1, HSPA1A, and UCK2 were the most abundant.

Conclusions: We identified 301 DAPs, which were enriched in olfactory transduction, glutathione metabolism, and calcium signaling pathways, suggesting the involvement of these processes in the onset of puberty. Further studies are warranted to more comprehensively explore the function of the identified DAPs and aforementioned signaling pathways to gain novel, deeper insights into the mechanisms underlying the onset of puberty.
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http://dx.doi.org/10.1186/s12864-022-08699-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281040PMC
July 2022

Correction: CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer.

Cell Death Dis 2022 Jul 12;13(7):598. Epub 2022 Jul 12.

Department of Clinical Laboratory, Shanghai Tenth People's Hospital of Tongji University, 200072, Shanghai, China.

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http://dx.doi.org/10.1038/s41419-022-05012-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276761PMC
July 2022

The expression of IGFBP-5 in the reproductive axis and effect on the onset of puberty in female rats.

Reprod Biol Endocrinol 2022 Jul 12;20(1):100. Epub 2022 Jul 12.

Anhui Provincial Key Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, 130 Changjiang West Road, Hefei, 230036, Anhui, China.

Insulin-like growth factor-binding protein-5 (IGFBP-5) has recently been shown to alter the reproductive capacity by regulating insulin-like growth factor (IGF) bioavailability or IGF-independent effects. The present study aimed to investigate the effect and mechanism of IGFBP-5 on the onset of puberty in female rats. Immunofluorescence and real-time quantitative PCR were used to determine the expression and location of IGFBP-5 mRNA and protein distribution in the infant's hypothalamus-pituitary-ovary (HPO) axis prepuberty, peripuberty, puberty and adult female rats. Prepubertal rats with IGFBP-5 intracerebroventricular (ICV) were injected to determine the puberty-related genes expression and the concentrations of reproductive hormones. Primary hypothalamic cells were treated with IGFBP-5 to determine the expression of puberty-related genes and the Akt and mTOR proteins. Results showed that Igfbp-5 mRNA and protein were present on the HPO axis. The addition of IGFBP-5 to primary hypothalamic cells inhibited the expression of Gnrh and Igf-1 mRNAs (P < 0.05) and increased the expression of AKT and mTOR protein (P < 0.01). IGFBP-5 ICV-injection delayed the onset of puberty, reduced Gnrh, Igf-1, and Fshβ mRNAs, and decreased the concentrations of E2, P4, FSH,serum LH levels and the ovaries weight (P < 0.05). More corpus luteum and fewer primary follicles were found after IGFBP-5 injection (P < 0.05).
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http://dx.doi.org/10.1186/s12958-022-00966-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277959PMC
July 2022

Golgi-Targeted Fluorescent Probe for Imaging NO in Alzheimer's Disease.

Anal Chem 2022 07 10;94(28):10256-10262. Epub 2022 Jul 10.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Nitric oxide (NO) is a crucial neurotransmitter participating in many biological processes via nitrosylation reaction. NO produced in diverse subcellular regions also regulates the function of cells in different manners. A Golgi apparatus is rich in nitric oxide synthase and may serve as a potential therapeutic target for Alzheimer's disease (AD). However, due to the lack of an effective tool, it is difficult to reveal the relationship between Golgi-NO and AD. Herein, we report Golgi-NO as the first Golgi-targeted fluorescent probe for sensing and imaging NO in the Golgi apparatus. The probe is designed and synthesized by incorporating 4-sulfamoylphenylamide as a Golgi-targeted moiety to 6-carboxyrhodamine B, generating a fluorophore of Golgi-RhB with modifiable carboxyl, which is then combined with the NO recognition moiety of -diaminobenzene. The probe shows superior analytical performance including accurate Golgi-targeted ability and high selectivity for NO. Moreover, using the probe, we disclose a significant increase of NO in Golgi apparatus in the AD model. This study provides a competent tool for studying the function and nitrosylation of NO in the Golgi apparatus in related diseases.
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http://dx.doi.org/10.1021/acs.analchem.2c01885DOI Listing
July 2022

Open Reduction of Displaced Radial Neck Fractures in Children by Internal Fixation Techniques: Comparison of Percutaneous Kirschner Wiring and Elastic Stable Intramedullary Nailing.

Indian J Orthop 2022 Jul 2;56(7):1192-1198. Epub 2022 Apr 2.

Department of Orthopaedics, Children's Hospital of Soochow University, Suzhou, 215000 Jiangsu Province China.

Background: Although most paediatric radial neck fractures can be treated with closed reduction, some severely displaced fractures require open reduction. The purpose of this study is to compare the effects of ESIN and KW fixation in open reduction of radial neck fracture in children.

Methods: Twenty-four patients with mean age of 8.5 years were included. Four of the patients had a Judet type III fracture and 20 had a Judet type IV fracture. Ten patients who underwent percutaneous KW fixation were assigned to group A, while 14 patients who underwent ESIN fixation were assigned to group B. Variables of interest included age, sex, fracture type, associated lesions, surgical time, fracture reduction, cost, follow-up, healing time, X-rays, clinical outcomes, and complications.

Results: There were no significant between-group differences in sex, age, additional injuries, fracture type, and quality of reduction. Costs were significantly lower in Group A. Fracture healing was achieved in 23 of 24 patients (10/10 in group A and 13/14 in group B). In a postoperative elbow function assessment based on the Steele and Graham classification, 80% of patients in group A had a score of excellent or good, compared to 78.6% of patients in group B. Two cases of nail shifting and joint protrusion were observed in group B, one of which also presented with nonunion during follow-up.

Conclusions: Both KW and ESIN may achieve good clinical outcomes, but KW is associated with lower costs, easier implant removal (without the need for a secondary surgery), and lower iatrogenic complications.
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http://dx.doi.org/10.1007/s43465-022-00631-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232670PMC
July 2022

The Effect of Narrative Nursing Intervention on Shame in Elderly Patients with Bladder Cancer after Ileal Bladder Replacement: A Cohort Study.

Comput Math Methods Med 2022 30;2022:4299919. Epub 2022 Jun 30.

Clinical Nursing Teaching and Research Section, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China.

Background: The standard treatment for bladder cancer (BC) is transurethral resection (TURBt), intravesical chemotherapy, and regular follow-up cystoscopy after surgery. However, some patients experience relapse or progression. Narrative care refers to a nursing model in which nurses put themselves into the patient's position through communication and listening, thereby alleviating the patient's negative emotions. This study analyzed narrative nursing interventions in elderly patients with BC after vesicoileal replacement.

Objective: To explore the positive stimulating effect of narrative nursing intervention on the sense of shame in elderly patients with bladder cancer (BC) after ileal bladder replacement.

Methods: A total of 60 elderly patients with BC who went through ileal replacement of the bladder from February 2019 to April 2021 in our hospital were enrolled. The patients were divided into the control group and the study group by the arbitrary number table method. The former group received routine care, and the latter group received a narrative nursing intervention model. The nursing satisfaction, stigma score, self-care ability score, SAS score, SDS score, and quality of life score were compared.

Results: First, we compared the nursing satisfaction. In the research group, 23 cases were very satisfied, 6 cases were satisfied, and 1 case was normal, and the satisfaction rate was 100.00%. In the control group, 13 cases were very satisfied, 8 cases were satisfied, 4 cases were general, and 5 cases were dissatisfied, with a satisfaction rate of 83.33%. The nursing satisfaction of the research group was significantly higher compared to that of the control group ( < 0.05). Secondly, we compared the stigma scores. The stigma scores of the study group at the time of discharge, 1 month, 3 months, and 6 months after discharge were lower compared to those of the control group ( < 0.05). In terms of the scores of self-care ability, the total scores of self-concept, self-care responsibility, self-care knowledge, self-care skills, and self-care ability of the research group were higher compared to those of the control group ( < 0.05). With regard to SAS scores, before nursing, there was no significant difference exhibited ( > 0.05). After nursing, the patient's SAS score decreased. Compared with the two groups, the SAS scores of the study group at discharge, 1 month, 3 months, and 6 months after discharge were all lower ( < 0.05). In terms of SDS score, there was no significant difference before nursing ( > 0.05). After nursing, the SDS scores of patients decreased. Compared between the two groups, the SDS scores of the study group at the time of discharge, 1 month, 3 months, and 6 months after discharge were lower ( < 0.05). Finally, we compared the life quality scores. Before nursing, there was no significant difference exhibited ( > 0.05). After nursing, the scores of life quality of patients improved. Compared with the two groups, the physical function, psychological function, social function, and healthy self-cognition scores of the research group were all lower compared to those of the control group ( < 0.05).

Conclusion: Narrative nursing can reduce anxiety and depression in elderly patients with BC after ileal replacement of the bladder, enhance the quality of life, reduce the patient's stigma, and play a positive motivating role. This nursing model is worthy of promotion in clinic.
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http://dx.doi.org/10.1155/2022/4299919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262506PMC
July 2022

pH-Induced reversible conversion between non-Pickering and Pickering high internal phase emulsion.

Phys Chem Chem Phys 2022 Jul 21;24(28):17121-17130. Epub 2022 Jul 21.

College of Science, Xihua University, Chengdu 610039, P. R. China.

Solid-stabilized high internal phase emulsions have received extensive attention. Many previous studies have confirmed that solid emulsifiers in high internal phase Pickering emulsions (HIPPEs) provide a great interface mechanical barrier. With the development of research, novel solid-stabilized emulsions have emerged. These emulsions are stabilized by the electrostatic repulsion between the surfactants and hydrophilic solid particles. They are distinct from Pickering emulsions in that the solid particles do not exist at the oil-water interface, but are dispersed in the continuous phase, so it is called a non-Pickering emulsion. However, high internal phase non-Pickering emulsions (HIPNPEs) are rarely reported. Herein, HIPNPEs that are synergistically stabilized by anionic surfactants with dynamic covalent bonds and negatively charged nano-SiO particles were prepared. In the presence of dodecylamine, the acidity causes the dynamic covalent bonds to break and the surfactant to be inactivated. Additionally, the long-chain amine is protonated and adsorbed on nano-SiO particles to form a new surfactant for stabilizing HIPPEs. However, alkalinity causes the HIPNPEs to form again. In addition, rheological tests confirmed that the HIPNPEs and HIPPEs had similar rheological behaviors, which were typical gel-like fluids. The emulsion can quickly respond to realize the conversion between the different types of high internal phase emulsion by simple stimulation, which provides a new direction for stimulus-responsive high internal phase emulsions.
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http://dx.doi.org/10.1039/d2cp01747dDOI Listing
July 2022

Systematic Pelvic and Para-Aortic Lymphadenectomy During Fertility-Sparing Surgery in Patients With Early-Stage Epithelial Ovarian Cancer: A Retrospective Study.

Front Oncol 2022 17;12:913103. Epub 2022 Jun 17.

Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, China.

Objective: The implication of pelvic and para-aortic lymphadenectomy in surgical staging of early-stage epithelial ovarian cancer (eEOC) is still debated. Limited data are available about systematic lymphadenectomy during fertility-sparing surgery (FSS) in patients with eEOC.

Methods: The medical records of 38 patients with FIGO stage I EOC and below 40 years who underwent FSS at our hospital between January 2003 and December 2018 were retrospectively reviewed.

Results: Among them, 18 patients (47.4%) underwent comprehensive lymphadenectomy, 11 patients (28.9%) underwent lymph node sampling, and 9 patients (23.7%) did not undergo lymphadenectomy. There was no statically significant difference in age, histology, grade, surgical approach, chemotherapy, and gestation among the three groups. With a median follow-up of 52.5 months (range: 24-153), three patients (7.9%) with FIGO stage IC EOC developed tumor recurrence. In these patients, progress-free survival (PFS) was 92.1%, and overall survival (OS) was 94.7%. No significant difference in the OS. Three patients had among all the patients, 15 patients (39.5%) had gestation after treatment, and 23 patients (60.5%) did not have gestation after treatment.

Conclusion: The number of lymph nodes removed did not significantly affect survival eEOC with FSS. Systematic pelvic and para-aortic lymphadenectomy could not be performed for mucious eEOC patients with FSS if intraoperative freezing in confirmed and no suspicious lymph nodes are found. A better understanding of sentinel lymph node biopsy may help to identify whether the patient requires FSS.
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http://dx.doi.org/10.3389/fonc.2022.913103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249102PMC
June 2022

Fungal Endophthalmitis in a Case of Rhino-Orbito-Cerebral Mucormycosis: Successfully Treated With Amphotericin B Colloidal Dispersion.

Front Microbiol 2022 15;13:910419. Epub 2022 Jun 15.

Department of Anesthesiology and Intensive Care, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Background: Rhino-orbito-cerebral mucormycosis (ROCM) is an acute, fulminant, opportunistic fungal infection that usually occurs in diabetes or immunocompromised patients. Amphotericin B combined with surgical debridement remains the standard treatment, although it is controversial due to its lager nephrotoxicity. Thus far, no studies have reported the treatment for ROCM-associated fungal endophthalmitis because the exact pathogenesis and transmission routes in ROCM remain unclear. Here, we reported a case of ROCM complicated with fungal endophthalmitis treated favorably with amphotericin B colloidal dispersion (ABCD) in combination with other antifungals and surgical debridement.

Case Presentation: A 34-year-old woman with diabetes was admitted to our hospital owing to right-sided headache for 8 days, blindness with swelling in the right eye for 5 days, and blindness in the left eye for 1 day. MRI showed that the patient had sphenoid sinus, sinuses, frontal lobe lesions, and proptosis of the right eye. Metagenomic sequencing revealed that the patient had infection. During hospitalization, the patient received intravenous ABCD, oral posaconazole, and topical amphotericin B and underwent surgical debridement. After 67 days of treatment, the patient's condition was significantly improved, and limb muscle strength showed grade V. showed negative results, and conjunctival swelling decreased. Additionally, no nephrotoxicity occurred during treatment. After discharge, the patient's treatment was transitioned to oral posaconazole and she was free of complaints during the 30-day follow-up without any additional treatment for ROCM.

Conclusion: Treatment with ABCD combined with other antifungal drugs and surgical debridement for ROCM complicated with fungal endophthalmitis showed remarkable efficacy and good safety. Hence, this regimen is a promising treatment strategy for this fatal disease.
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http://dx.doi.org/10.3389/fmicb.2022.910419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240434PMC
June 2022

Dietary Magnesium Intake Level Modifies the Association Between Vitamin D and Insulin Resistance: A Large Cross-Sectional Analysis of American Adults.

Front Nutr 2022 7;9:878665. Epub 2022 Jun 7.

Department of Basic Medical Sciences, Qinghai University Medical College, Xining, China.

Background: Previous clinical studies and randomized controlled trials have revealed that low serum vitamin D levels are associated with the risk of developing insulin resistance. Magnesium has been reported to be a protective factor for insulin resistance, and magnesium has been considered an important co-factor for vitamin D activation. However, the effect of dietary magnesium intake on the relationship between vitamin D and the risk of developing insulin resistance has not been comprehensively investigated. Therefore, we designed this cross-sectional analysis to assess whether dietary magnesium intake modifies the association of vitamin D and insulin resistance.

Methods: A total of 4,878 participants (male: 48.2%) from 4 consecutive cycles of the National Health and Nutrition Examination Survey (2007-2014) were included in this study after a rigorous screening process. Participants were stratified by their dietary magnesium intake into low-intake (<267 mg/day) and high-intake (≥267 mg/day) groups. We assessed differences between serum vitamin D levels and the risk of developing insulin resistance (interaction test), using a weighted multivariate logistic regression to analyze differences between participants with low and high magnesium intake levels.

Results: There was a negative association between vitamin D and insulin resistance in the US adult population [OR: 0.93 (0.88-0.98)], < 0.001. Dietary magnesium intake strengthened the association ( for interaction < 0.001). In the low dietary magnesium intake group, vitamin D was negatively associated with the insulin resistance [OR: 0.94 (0.90-0.98)]; in the high dietary magnesium intake group, vitamin D was negatively associated with insulin resistance [OR: 0.92 (0.88-0.96)].

Conclusion: Among adults in the United States, we found an independent association between vitamin D level and insulin resistance, and this association was modified according to different levels of magnesium intake.
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http://dx.doi.org/10.3389/fnut.2022.878665DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9211020PMC
June 2022

Swimming Suppresses Cognitive Decline of HFD-Induced Obese Mice through Reversing Hippocampal Inflammation, Insulin Resistance, and BDNF Level.

Nutrients 2022 Jun 11;14(12). Epub 2022 Jun 11.

Tianjiu Research and Development Center for Exercise Nutrition and Foods, Hubei Key Laboratory of Exercise Training and Monitoring, College of Health Science, Wuhan Sports University, Wuhan 430079, China.

Obesity is an important public health problem nowadays. Long-term obesity can trigger a series of chronic diseases and impair the learning and memory function of the brain. Current studies show that scientific exercise can effectively improve learning and memory capacity, which also can provide benefits for obese people. However, the underlying mechanisms for the improvement of cognitive capacity under the status of obesity still need to be further explored. In the present study, the obesity-induced cognition-declined model was established using 4-week-old mice continuously fed with a high-fat diet (HFD) for 12 weeks, and then the model mice were subjected to an 8-week swimming intervention and corresponding evaluation of relevant indicators, including cognitive capacity, inflammation, insulin signal pathway, brain-derived neurotrophic factor (BNDF), and apoptosis, for exploring potential regulatory mechanisms. Compared with the mice fed with regular diets, the obese mice revealed the impairment of cognitive capacity; in contrast, swimming intervention ameliorated the decline in cognitive capacity of obese mice by reducing inflammatory factors, inhibiting the JNK/IRS-1/PI3K/Akt signal pathway, and activating the PGC-1α/BDNF signal pathway, thereby suppressing the apoptosis of neurons. Therefore, swimming may be an important interventional strategy to compensate for obesity-induced cognitive impairment.
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http://dx.doi.org/10.3390/nu14122432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228449PMC
June 2022

Epidemiology, Clinical Characteristics, Risk Factors, and Outcomes of Candidemia in a Large Tertiary Teaching Hospital in Western China: A Retrospective 5-Year Study from 2016 to 2020.

Antibiotics (Basel) 2022 Jun 9;11(6). Epub 2022 Jun 9.

Division of Clinical Microbiology, Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu 610041, China.

The aim of this study was to investigate the current status of candidemia and evaluate the clinical characteristics, risk factors and outcomes among different species. We conducted a retrospective study by univariate and multivariate analysis between and (NAC) species in a Chinese national medical center from 2016 to 2020. Among the 259 episodes, (38.6%) was the leading species, followed by (24.3%), (20.5%), and (12.4%). Most and were susceptible to nine tested antifungal agents, whereas showed 30.2~65.9% resistance/non-wild-type to four azoles with great cross-resistance, indicating that fluconazole should not be used for empirical antifungal treatment. In multivariable models, the factor related to an increased risk of NAC was glucocorticoid exposure, whereas gastrointestinal hemorrhage and thoracoabdominal drainage catheters were associated with an increased risk in . Subgroup analysis revealed leukemia and lymphoma, as well as glucocorticoid exposure, to be factors independently associated with in comparison with candidemia. No significant differences in 7-day mortality or 30-day mortality were observed between and NAC. This study may provide useful information with respect to choosing empirical antifungal agents and exploring differences in molecular mechanisms.
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http://dx.doi.org/10.3390/antibiotics11060788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220019PMC
June 2022

Long Noncoding RNA BCYRN1 Recruits BATF to Promote TM4SF1 Upregulation and Enhance HCC Cell Proliferation and Invasion.

Dis Markers 2022 11;2022:1561607. Epub 2022 Jun 11.

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.

Hepatocellular carcinoma (HCC) is a common form of cancer for which a subset of reliable clinical biomarkers has been defined. However, other factors including long noncoding RNAs (lncRNAs) can also regulate HCC development. This study was thus designed to understand how the lncRNA Brain cytoplasmic RNA 1 (BCYRN1) modulates HCC progression. Bioinformatics approaches were used to identify genes, lncRNAs, and transcription factors that were differentially expressed in the context of HCC, after which the relative expression of BCYRN1 in HCC and control tissues was assessed via qPCR. The ability of BCYRN1 to bind the transcription factor BATF was further evaluated in an RNA immunoprecipitation (RIP) assay, while chromatin immunoprecipitation (ChIP) was used to gauge the binding of the TM4SF1 promoter by BATF. Luciferase reporter assays were also used to assess the association between BCYRN1 and the TM4SF1 promoter. Subsequent loss- and gain-of-function assays were then conducted to explore the effects of altering BCYRN1 expression levels on the proliferative, invasive, and migratory activity of HCC cells. BCYRN1 upregulation was associated with poorer clinical outcomes in HCC patients, and knocking down this lncRNA impaired HCC cell migration and invasion. From a mechanistic perspective, BATF was recruited to the TM4SF1 promoter by BCYRN1, and reducing the expression of this lncRNA was sufficient to constrain xenograft tumor growth in mice. These results highlight BCYRN1 as a putative therapeutic target in HCC tumors.
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http://dx.doi.org/10.1155/2022/1561607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206761PMC
June 2022

Separate Reclamation of Oil and Surfactant from Oil-in-Water Emulsion with a CO-Responsive Material.

Environ Sci Technol 2022 Jul 20;56(13):9651-9660. Epub 2022 Jun 20.

Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Oil-in-water (O/W) emulsion is one type of oily wastewater produced by many industries. The treatment of and resource recovery from O/W emulsions are very challenging. Unlike bulk or floating oil, which can be successfully abstracted from wastewater by hydrophobic/oleophilic materials, the abstraction of emulsified oil is not easy because of its highly hydrophilic surface composed of dense surfactants. Separate reclamation of miscible oil and surfactant through a green approach is even more difficult. Here, we report that a CO-responsive material can abstract emulsified oil and demulsify the oil droplets. Moreover, it can release the abstracted oil and surfactant separately. This material exhibited a very high adsorption capacity for emulsified oil (14 g g). Upon switching the surface wettability of the material under CO or synthetic flue gas sparging, coalesced oil was reclaimed while the surfactant was retained inside the pores. The hydrophobic character of the material was retrieved when CO was purged with nitrogen sparging or air heating. Then, the surfactant was reclaimed by elution with diluted alkali/ethanol. Oil and surfactant were thus separately reclaimed from the O/W emulsion. High rates of oil removal, oil recovery, and surfactant recovery were maintained during repeated adsorption/desorption operations. This work provides a potentially sustainable and green way for O/W emulsion treatment and resource recovery.
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http://dx.doi.org/10.1021/acs.est.1c08149DOI Listing
July 2022

pH-Responsive Regulation of a Surfactant-Free Microemulsion Based on Hydrophobic Deep Eutectic Solvents.

Langmuir 2022 Jul 18;38(26):7898-7905. Epub 2022 Jun 18.

School of New Energy and Materials, Southwest Petroleum University, Chengdu 610500, P. R. China.

Microemulsions containing a responsive hydrophobic deep eutectic solvent (HDES) as the oil phase that can replace conventional organic solvents are considered to be a green strategy. It is anticipated that a pH-responsive HDES is synthesized to prepare rapid responsive surfactant-free microemulsions (SFMEs), which enable the transition from SFMEs to nanoemulsions. Menthol and -octanoic acid (OA) were assembled into HDES by hydrogen bonding at a molar ratio of 1:2. The pH-responsive HDES as the oil phase and isopropyl alcohol (IPA) as the double solvent could form HDES/IPA/water SFMEs, which have unique responsiveness. Specifically, from the nuclear magnetic resonance hydrogen spectrum, pH, thermogravimetry, and Fourier transform infrared spectroscopy investigations, the excellent switchability and stability of menthol-OA were demonstrated. On the basis of these complexes, microemulsions were successfully prepared. Electrical conductivity and pH measurements were used to determine the structures of microemulsions and the phase inversion process. The effects of the contents of water and HDES, NaCl concentration, and pH of the system were investigated. Nanoemulsions were successfully prepared on the basis of the pH response of the microemulsions. In addition, the prepared nanoemulsion has a unique pH-responsive behavior that can be controllably regulated among nanoemulsions, microemulsions, and phase separation systems.
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http://dx.doi.org/10.1021/acs.langmuir.2c00181DOI Listing
July 2022

Machine Learning Advances in Microbiology: A Review of Methods and Applications.

Front Microbiol 2022 26;13:925454. Epub 2022 May 26.

State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao, China.

Microorganisms play an important role in natural material and elemental cycles. Many common and general biology research techniques rely on microorganisms. Machine learning has been gradually integrated with multiple fields of study. Machine learning, including deep learning, aims to use mathematical insights to optimize variational functions to aid microbiology using various types of available data to help humans organize and apply collective knowledge of various research objects in a systematic and scaled manner. Classification and prediction have become the main achievements in the development of microbial community research in the direction of computational biology. This review summarizes the application and development of machine learning and deep learning in the field of microbiology and shows and compares the advantages and disadvantages of different algorithm tools in four fields: microbiome and taxonomy, microbial ecology, pathogen and epidemiology, and drug discovery.
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http://dx.doi.org/10.3389/fmicb.2022.925454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196628PMC
May 2022

CCCTC-Binding Factor Mediates the Transcription of Insulin-Like Growth Factor Binding Protein 5 Through EZH2 in Ulcerative Colitis.

Dig Dis Sci 2022 Jun 15. Epub 2022 Jun 15.

Department of Anorectal Surgery, Hengyang Medical School, The Affiliated Nanhua Hospital, University of South China, No. 336, Dongfeng South Road, Zhuhui District, Hengyang, 421001, Hunan, People's Republic of China.

Background: Ulcerative colitis (UC) features chronic, non-infectious inflammation of the colon. Insulin-like growth factor binding protein 5 (IGFBP5) has been indicated to be related to various inflammation-related diseases. However, its association with UC remains largely unclear.

Aims: Here, we investigate the role of IGFBP5 in colonic mucosal epithelial cell injury in UC.

Methods: Differentially expressed genes in the colonic tissues of UC mice were screened using the Gene Expression Omnibus database, and IGFBP5 was identified. UC mice were developed using dextran sulfate sodium, and IGFBP5 expression in the colonic tissues of UC mice was confirmed by immunohistochemistry and RT-qPCR. The effects of IGFBP5 in vivo and in vitro were investigated by intraperitoneal injection of adeno-associated virus into UC mice or by transfection with an IGFBP5 overexpression plasmid into lipopolysaccharide-treated colonic mucosal epithelial cells. The mechanisms causing IGFBP5 deletion in UC were predicted by bioinformatics analysis and ChIP-qPCR and verified by rescue experiments.

Results: IGFBP5 was reduced in UC. IGFBP5 impaired the NFκB pathway in the colonic tissue of UC mice and ameliorated inflammatory infiltration and colonic mucosal cell injury. IGFBP5 depletion was associated with H3K27me3 modification, which was induced by EZH2. CTCF was responsible for recruiting EZH2 to the promoter region of IGFBP5. CTCF inhibition repressed UC progression by reducing H3K27me3 modification via the discouragement of the enrichment of EZH2 in the IGFBP5 promoter.

Conclusions: CTCF modulates H3K27me3 modification of the IGFBP5 promoter by recruiting EZH2, thereby downregulating IGFBP5 to accentuate colonic mucosal epithelial cell injury in UC mice.
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http://dx.doi.org/10.1007/s10620-022-07566-wDOI Listing
June 2022

A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.

Cell Metab 2022 Aug 14;34(8):1168-1182.e6. Epub 2022 Jun 14.

Department of Endocrinology, Endocrinology Research Center, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China; Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Hunan 410008, China. Electronic address:

Exercise can prevent osteoporosis and improve immune function, but the mechanism remains unclear. Here, we show that exercise promotes reticulocalbin-2 secretion from the bone marrow macrophages to initiate bone marrow fat lipolysis. Given the crucial role of lipolysis in exercise-stimulated osteogenesis and lymphopoiesis, these findings suggest that reticulocalbin-2 is a pivotal regulator of a local adipose-osteogenic/immune axis. Mechanistically, reticulocalbin-2 binds to a functional receptor complex, which is composed of neuronilin-2 and integrin beta-1, to activate a cAMP-PKA signaling pathway that mobilizes bone marrow fat via lipolysis to fuel the differentiation and function of mesenchymal and hematopoietic stem cells. Notably, the administration of recombinant reticulocalbin-2 in tail-suspended and old mice remarkably decreases bone marrow fat accumulation and promotes osteogenesis and lymphopoiesis. These findings identify reticulocalbin-2 as a novel mechanosensitive lipolytic factor in maintaining energy homeostasis in bone resident cells, and it provides a promising target for skeletal and immune health.
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http://dx.doi.org/10.1016/j.cmet.2022.05.009DOI Listing
August 2022

A functionalized magnetic nanoparticle regulated CRISPR-Cas12a sensor for the ultrasensitive detection of alpha-fetoprotein.

Analyst 2022 Jul 12;147(14):3186-3192. Epub 2022 Jul 12.

Department of Clinical Laboratory, Shanghai Tenth People's Hospital Chong Ming Branch, 202150, China.

Alpha-fetoprotein (AFP) is an important clinical tumor marker of hepatoblastoma, and the concentration of AFP in serum is closely related to the staging of hepatoblastoma. We report a magnetic bead separation platform based on a switching aptamer triggered hybridization chain reaction (SAT-HCR) and the CRISPR-Cas12a sensor for the detection of AFP. AFP aptamer, as an easily regulated nucleic acid strand, is responsible for binding to AFP into nucleic acid detection, while HCR-CRISPR-Cas12a, regulated by functionalized magnetic nanoparticles, is responsible for highly specific nucleic acid signal amplification. Under the optimal conditions, the fluorescence intensity was proportional to the concentration of AFP in the range of 0.5-10 ng mL and the limit of detection was 0.170 ng mL. In addition, we have successfully applied this biosensor to detect AFP in clinical samples from patients with hepatoblastoma, with greater sensitivity relative to ELISA. Our proposed method showed great potential application in clinical diagnosis and pharmaceutical-related fields with the properties of high sensitivity, low cost and high selectivity.
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http://dx.doi.org/10.1039/d2an00697aDOI Listing
July 2022

The B-Type Cyclin CYCB1-1 Regulates Embryonic Development and Seed Size in Maize.

Int J Mol Sci 2022 May 25;23(11). Epub 2022 May 25.

Key Laboratory of Crop Physiology, Ecology and Genetic Breeding Ministry of Education, College of Agronomy, Jiangxi Agricultural University, Nanchang 330046, China.

Progress through the cell cycle is a critical process during plant embryo and seed development and its progression is regulated by cyclins. Despite extensive study of cyclins in other systems, their role in embryo and seed development of maize is unclear. In this study, we demonstrate that overexpression significantly accelerated embryo growth and increased seed size. In situ hybridization and toluidine blue staining indicated that was highly expressed in the plumule of embryos, and the cells of the plumule were smaller, denser, and more regularly arranged in overexpression plants. Overexpression of in maize also resulted in an increased ear length and enhanced kernel weight by increasing kernel width. Transcriptome analysis indicated that the overexpression of affected several different metabolic pathways, including photosynthesis in embryos and leaves, and lipid metabolism in leaves. Conversely, knocking out resulted in plants with slow growth. Our results suggest that regulates embryo growth and seed size, making it an ideal target for efforts aimed at maize yield improvement.
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http://dx.doi.org/10.3390/ijms23115907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180882PMC
May 2022

iTRAQ-based quantitative proteomic analysis provides insight into the drought-stress response in maize seedlings.

Sci Rep 2022 06 9;12(1):9520. Epub 2022 Jun 9.

Maize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Shuguang Garden Middle Road No. 9, Beijing, 100097, China.

Drought is a major abiotic stress that harms plant cell physiology and limits the growth and productivity of crops. Maize (Zea mays L.), one of the most drought-susceptible crops, is a major food source for humans and an important resource for industrial bioenergy production; therefore, understanding the mechanisms of the drought response is essential for maize improvement. Using isotopic tagging relative quantitation (iTRAQ)-based protein labeling technology, we detected the proteomic changes in maize leaves under drought stress. Among the 3063 proteins that were identified, the abundance of 214 and 148 proteins increased and decreased, respectively, after three days of drought treatment. These differentially abundant proteins (DAPs) were mainly involved in cell redox homeostasis, cell wall organization, photosynthesis, abscisic acid biosynthesis, and stress-response processes. Furthermore, some of the DAP abundances still differed from the control six days after the drought treatment, most of which were molecular chaperones, heat shock proteins, metabolism-related enzymes, hydrolases, and transmembrane signal receptors. The expression level of some DAPs returned to normal when the water supply was restored, but for others it did not. A significant correlation between the protein and transcript levels was observed following an RT-qPCR analysis. Finally, our research provides insights into the overall mechanism of drought-stress tolerance, and important information for breeding of drought-tolerant maize.
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http://dx.doi.org/10.1038/s41598-022-13110-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184573PMC
June 2022

The genomic landscape of Cronkhite-Canada syndrome: Possible clues for pathogenesis.

J Dig Dis 2022 Jun 9. Epub 2022 Jun 9.

Department of Gastroenterology, Chinese Academy of Medical Sciences, Peking Union Medical College Hospital, Beijing, China.

Objectives: Cronkhite-Canada syndrome (CCS) is a rare hamartomatous polyposis syndrome with a proposed association with chronic autoimmune inflammation. To date, genetic background of patients with CCS remains less investigated. In this study we aimed to explore the genomic landscape of CCS.

Methods: Whole exome sequencing was performed on peripheral blood samples extracted from 18 patients with CCS. Potential function-impacting germline variants were filtered by R software. Through systematic data analysis, a number of genetic variants were identified. Enrichment analysis was performed using the R package ClusterProfiler.

Results: Overall, 3960 low-frequency (<0.05 or not reported in the Exome Aggregation Consortium East Asian, 1000 Genomes, or ESP6500 database) potentially function-impacting germline variants were identified, with 18 genes (FDFT1, LOC400863, MUC3A, MUC4, ZNF806, GXYLT1, MUC6, PABPC3, PSPH, ZFPM1, CIC, LOC283710, ARSD, GOLGA6L2, LOC388282, SLC25A5, TMEM247, WDR89) involved over half the patients. Functional enrichment of these genes revealed several biological processes in relation to innate immune responses and glycosylation. Only one likely pathogenic germline variant of an hamartomatous polyposis syndrome-associated gene, PTCH1, was detected in one patient.

Conclusions: CCS has genomic alteration patterns completely distinct from those of traditional hamartomatous polyposis syndrome. The germline mutation landscape indicates potential roles of innate immune responses and glycosylation in the pathogenesis of CCS.
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http://dx.doi.org/10.1111/1751-2980.13101DOI Listing
June 2022
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