Publications by authors named "Ya Li"

858 Publications

Malignant peritoneal mesothelioma.

Am J Med Sci 2022 Aug 5. Epub 2022 Aug 5.

Department of Pathology and Medical Research Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China. Electronic address:

Malignant peritoneal mesothelioma (MPM) is a rare, life-threatening malignant tumor. We present a report of a rare case of a 67-year-old male patient with MPM and severe abdominal pain, bloating, and bloody ascites as manifestations. The diagnosis was confirmed by cytology of ascites aspiration fluid and further verified by laparoscopic exploratory biopsy. The characteristics of signs and clinical manifestations in this case are less common. As everyone knows, asbestos exposure is usually associated with pleural mesothelioma, but only 6%-10% of malignant mesothelioma cases originate from the peritoneum, which is far less than pleural mesothelioma. Generally, its non-specificity provides a huge challenge to medical professionals in its diagnosis, and this is also the main reason for delayed diagnosis. Patients should be vigilant, even though no clear risk factor is observed.
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http://dx.doi.org/10.1016/j.amjms.2022.07.008DOI Listing
August 2022

Small Cell (Neuroendocrine) Carcinoma of the Cervix: An Analysis for 19 Cases and Literature Review.

Front Cell Infect Microbiol 2022 13;12:916506. Epub 2022 Jul 13.

Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.

Cervical SCNEC is a rare and highly malignant invasive tumor. The incidence is low, at less than 5% of all cervical cancers. Moreover, most patients with small cell carcinoma are interrelated with high risk HPV (more familiar HPV 18). Compared to squamous cell carcinoma or adenocarcinoma, patients of cevical SCNEC are more prone to lymph node invasion early, so the clinical manifestation is usually local or distant metastasis. We summarized the clinical features of 19 patients with cervical small cell carcinoma in the Second Affiliated Hospital of Dalian Medical University from 2012 to 2021, and retrospectively analyzed data from 1576 patients in 20 related studies and more than 50 pieces of literature in recent years by searching PubMed, Google schalor, Cochrane Library, Clinicalkey, and other databases. The collected patient data included age, clinical manifestation, TCT, HPV detection, the size and morphology of the tumor, local invasion depth, stage, lymph node status, initial treatment method, tumor-free survival, and so on. The positive rates of CGA, SYN, and CD56 in our cases were high, and NSE was a moderately sensitive index. P16 and Ki67 were the most sensitive, and all patients were positive. We found that multimodal treatment can indeed improve tumor-free survival (DFS), but the prognosis of patients is still very poor. For the early stages, our treatment principles refer to the guidelines of SGO, international gynecological cancer Cooperation (GCIG), and NCCN. We suggest a combination of surgery, radiotherapy, and chemotherapy. However, the general state of advanced patients is poor, whether they can tolerate the operation after neoadjuvant chemotherapy, whether the operation area can remain tumor-free, and whether this treatment will prolong the survival time of patients still need to be further discussed. In order to better prolong the tumor-free survival and prognosis of patients, we need to find gene changes suitable for targeted therapy, so as to complete the clinical application of these treatment methods. Further works are needed to explore more effective therapy for cervical SCNEC.
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http://dx.doi.org/10.3389/fcimb.2022.916506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326003PMC
August 2022

Multi-mtDNA Variants May Be a Factor Contributing to Mitochondrial Function Variety in the Skin-Derived Fibroblasts of Leber's Hereditary Optic Neuropathy Patients.

Front Mol Neurosci 2022 13;15:920221. Epub 2022 Jul 13.

Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, China.

Heterogeneity is a major feature of Leber's hereditary optic neuropathy (LHON) and has a significant impact on the manifestation and diagnosis of the disease. This study explored whether multiple variations in mitochondrial genes were associated with the heterogeneity, mainly phenotypic heterogeneity. Ophthalmic examinations were conducted in two probands with LHON with G11778A and multiple mitochondrial DNA gene (mtDNA) variants. Skin fibroblast cell lines were generated from patients and age- and sex-matched controls. ROS levels, mitochondrial membrane potential, cell energy respiration, and metabolic functions were measured. Flow cytometry and cell viability tests were performed to evaluate the cell apoptosis levels and fate. We found that cells with more mtDNA variants had higher ROS levels, lower mitochondrial membrane potential, and weaker respiratory function. Flow cytometry and cell viability testing showed that multiple mtDNA variants are associated with different levels of cell viability and apoptosis. In conclusion, we found that skin-derived fibroblast cells from G11778A LHON patients could be used as models for LHON research. Multi-mtDNA variants contribute to mitochondrial function variety, which may be associated with heterogeneity in patients with LHON.
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http://dx.doi.org/10.3389/fnmol.2022.920221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326446PMC
July 2022

Clinical Application of Different Vertical Position Tests for Posterior Canal-Benign Paroxysmal Positional Vertigo-Cupulolithiasis.

Front Neurol 2022 12;13:930542. Epub 2022 Jul 12.

Department of Neurology, The First Hospital of Hebei Medical University, Shijiazhuang, China.

Background: Posterior canal-benign paroxysmal positional vertigo-cupulolithiasis (PC-BPPV-cu) is a new and controversial type of benign paroxysmal positional vertigo (BPPV). At present, there are few relevant clinical studies as to whether the Half Dix-Hallpike test (Half D-HT) induces more obvious nystagmus than the Dix Hallpike test (D-HT) and straight head hanging test (SHH) in patients with PC-BPPV-cu.

Objectives: To investigate the clinical characteristics of PC-BPPV-cu, and analyze the diagnostic significance of the Dix-Hallpike test (D-HT), Half D-HT, and straight head hanging (SHH) test in these patients.

Methods: A total of 46 patients with PC-BPPV-cu were enrolled, and divided into two groups ( = 23): a group A (induction order: D-HT, Half D-HT, SHH) and a group B (induction order: Half D-HT, D-HT, SHH).

Results: Among 46 patients with PC-BPPV-cu, the bilateral and unilateral abnormality rates of the disease side were 5 cases and 41 cases, respectively. There were significant differences in the proportion of torsional-upbeating nystagmus and upbeating nystagmus among the three headhanging positions in 46 patients with PC-BPPV-cu ( < 0.001). The slow phase velocity (SPV) of induced nystagmus at half D-HT supine position was slower than D-HT supine position ( < 0.05) and SHH supine position ( < 0.05). The nystagmus latency of D-HT supine position was significantly shorter than half D-HT ( < 0.05) and SHH ( < 0.05). PC-BPPV-cu patients were accompanied by 53.5% semicircular canal paresis, 69.6% audiological abnormalities, 76% cervical vestibular evoked myogenic potential (cVEMP), and 75% video head impulse test (vHIT) abnormalities, the concordance rates of the four detection methods were similar (χ = 0.243, = 0.970).

Conclusions: The Half D-HT is simple and feasible, but might have a risk of false-negative diagnoses of the torsional-upbeating nystagmus and upbeating nystagmus. The D-HT is still a classic induction method for PC-BPPV-cu. The two complement each other and may aid in the diagnosis of PC-BPPV-cu patients. Future clinical applications of Half D-HT require extensive research to determine its diagnostic efficacy.
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http://dx.doi.org/10.3389/fneur.2022.930542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315446PMC
July 2022

Autosomal dominant optic atrophy caused by six novel pathogenic OPA1 variants and genotype-phenotype correlation analysis.

BMC Ophthalmol 2022 Jul 26;22(1):322. Epub 2022 Jul 26.

Department of Ophthalmology, Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Zhengzhou University, Zheng-zhou, 450003, China.

Purpose: To describe the genetic and clinical features of nineteen patients from eleven unrelated Chinese pedigrees with OPA1-related autosomal dominant optic atrophy (ADOA) and define the phenotype-genotype correlations.

Methods: Detailed ophthalmic examinations were performed. Targeted next-generation sequencing (NGS) was conducted in the eleven probands using a custom designed panel PS400. Sanger sequencing and cosegregation were used to verify the identified variants. The pathogenicity of gene variants was evaluated according to American College of Medical Genetics and Genomics (ACMG) guidelines.

Results: Nineteen patients from the eleven unrelated Chinese ADOA pedigrees had impaired vision and optic disc pallor. Optical coherence tomography showed significant thinning of the retinal nerve fiber layer. The visual field showed varying degrees of central or paracentral scotoma. The onset of symptoms occurred between 3 and 24 years of age (median age 6 years). Eleven variants in OPA1 were identified in the cohort, and nine novel variants were identified. Among the novel variants, two splicing variants c.984 + 1_984 + 2delGT, c.1194 + 2 T > C, two stop-gain variants c.1937C > G, c.2830G > T, and one frameshift variant c.2787_2794del8, were determined to be pathogenic based on ACMG. A novel splicing variant c.1316-10 T > G was determined to be likely pathogenic. In addition, a novel missense c.1283A > C (p.N428T) and two novel splicing variants c.2496G > A and c.1065 + 5G > C were of uncertain significance.

Conclusions: Six novel pathogenic variants were identified. The findings will facilitate genetic counselling by expanding the pathogenic mutation spectrum of OPA1.
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http://dx.doi.org/10.1186/s12886-022-02546-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327245PMC
July 2022

General Pharmacological Activation Mechanism of K Channels Bypassing Channel Gates.

J Med Chem 2022 Jul 25. Epub 2022 Jul 25.

Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, China.

Under the known pharmacological activation mechanisms, activators allosterically or directly open potassium channel gates. However, herein, molecular dynamics simulations on TREK-1, a member of the channel class gated at the filter, suggested that negatively charged activators act with a gate-independent mechanism where compounds increase currents by promoting ions passing through the central cavity. Then, based on studies of KCNQ2, we uncovered that this noncanonical activation mechanism is shared by the other channel class gated at the helix-bundle crossing. Rational drug design found a novel KCNQ2 agonist, CLE030, which stably binds to the central cavity. Functional analysis, molecular dynamics simulations, and calculations of the potential of mean force revealed that the carbonyl oxygen of CLE030 influences permeant ions in the central cavity to contribute to its activation effects. Together, this study discovered a ligand-to-ion activation mechanism for channels that bypasses their gates and thus is conserved across subfamilies with different gates.
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http://dx.doi.org/10.1021/acs.jmedchem.1c02115DOI Listing
July 2022

Gensini score values for predicting periprocedural myocardial infarction: An observational study analysis.

Medicine (Baltimore) 2022 Jul 22;101(29):e29491. Epub 2022 Jul 22.

Department of Cardiology, Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Zhejiang University, Hangzhou, People's Republic of China.

The Gensini score (GS) is a convenient, powerful tool for assessing the severity and complexity of coronary artery diseases. Our research investigated the relationship between the GS and periprocedural myocardial infarction (PMI). We recruited 4949 patients (3366 men, 1583 women; mean age 66.45 ± 10.09 years) with a single coronary artery revascularization. Based on the tertile of the GS 20 and 36, the population was divided into 3 groups: Low Group (0 < GS ≤ 20, N = 1809); Intermediate Group (20 < GS ≤ 36, N = 1579); High Group (GS > 36, N = 1561). PMI3 represented the endpoint for cTnI > 3-fold upper reference limit, while PMI5 represented the endpoint for cTnI > 5-fold upper reference limit. The incidence of PMI of High Group was statistically higher than that of Intermediate Group (P < .05), while that of Intermediate Group was statistically higher than Low Group (P < .05). With the adjustment of some general variables, GS was an independent significantly predictor for PMI3 (β = 0.006, P < .05) and PMI5 (β = 0.007, P < .05). Following receiver operating characteristic curve analysis, the optimal cut-off value to predict PMI are 22.5 for PMI3 and 27 for PMI5. The GS was an independent predictor of PMI in the single-coronary revascularization population. Additionally, the 22.5 of GS was the optimal cut-off value for determining the presence of PMI3, while the 27 of GS for PMI5.
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http://dx.doi.org/10.1097/MD.0000000000029491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302281PMC
July 2022

Accurate age estimation from blood samples of Han Chinese individuals using eight high-performance age-related CpG sites.

Int J Legal Med 2022 Jul 11. Epub 2022 Jul 11.

Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, No. 13, Hangkong Road, Wuhan, 430030, China.

Age-related CpG sites (AR-CpGs) are currently the most promising biomarkers for forensic age estimation. In our previous studies, we first validated the age correlation of seven reported AR-CpGs in blood samples of Chinese Han population. Subsequently, we screened some good age predictors from blood samples of Chinese Han population, and built pyrosequencing-based age prediction models. However, it is still important to select a set of high-performance AR-CpGs in a specific racial group and establish a simple and efficient method for accurate age estimation for forensic purpose. In this study, eight AR-CpGs, namely chr6: 11,044,628 (ELOVL2), cg06639320 (FHL2), chr1: 207,823,723 (C1orf132), cg19283806 (CCDC102B), cg14361627 (KLF14), cg17740900 (SYNE2), cg07553761 (TRIM59), and cg26947034, were selected based on our previous studies, and a multiplex methylation SNaPshot assay was developed to investigate DNA methylation levels at these AR-CpGs in 529 blood samples (aged 2-82 years) from Han Chinese population. All selected CpG sites showed strong age correlation with the correlation coefficient (r) from 0.8363 to 0.9251. Multiple linear regression (MLR) and support vector regression (SVR) age prediction models were simultaneously established to fit change characteristics of DNA methylation levels of eight AR-CpGs with the age in 374 donors' blood samples. The MLR model enabled age prediction with R = 0.923, mean absolute error (MAE) = 3.52, while the SVR model enabled age prediction with R = 0.935, MAE = 2.88. One hundred fifty-five independent samples were used as a validation set to test the two models' performance, and the prediction MAE for the validation set was 3.71 and 3.34 for the MLR and SVR models, respectively. For the MLR and SVR models, the correct prediction rate at ± 5 years reached a high level of 79.35% and 83.23%, respectively. In general, these statistical parameters indicated that the SVR model outperformed the MLR model in age prediction of the Han Chinese population. In addition, our method provides sufficient sensitivity in forensic applications and allows for 100% efficiency when examining bloodstains kept in room conditions for up to 43 days. These results indicate that our multiplex methylation SNaPshot assay is a reliable, effective, and accurate method for age prediction in blood samples from the Chinese Han population.
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http://dx.doi.org/10.1007/s00414-022-02865-3DOI Listing
July 2022

Unity of Opposites between Soluble and Insoluble Lithium Polysulfides in Lithium-Sulfur Batteries.

Adv Mater 2022 Jul 11:e2203699. Epub 2022 Jul 11.

Key Laboratory of Core Technology of High Specific Energy Battery and Key Materials for Petroleum and Chemical Industry, College of Energy, Soochow University, Suzhou, Jiangsu, 215006, P. R. China.

Rechargeable batteries based on Li-S chemistry show promise as possible for next-generation energy storage devices because of their ultra-high capacities and energy densities. Research over the past decade has demonstrated that the morphology of lithium polysulfides (LPSs) in electrolytes (soluble or insoluble) plays a decisive role in battery performance. Early studies have focused mainly on inhibiting the dissolution of LPSs and invested considerable effort to realize this objective. However, in recent years, a completely different view has emerged that the dissolution of LPSs during battery discharge/charge should be promoted. At this critical juncture in the large-scale application of Li-S batteries, it is time to summarize and discuss both sides of the contradiction. This perspective provides an overview of these two opposite views pertaining to soluble and insoluble LPSs, including their historical environment, classical strategies, advantages, and disadvantages. Finally, the future morphology of LPSs in Li-S batteries is predicted based on a multi-angle review of research studies conducted thus far, and the reasoning behind this conjecture is thoroughly discussedi. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202203699DOI Listing
July 2022

An evidence-based evaluation of Buyang Huanwu decoction for the treatment of the sequelae of stroke: A PRISMA-compliant systematic review and meta-analysis of randomized controlled trials.

Phytomedicine 2022 Sep 3;104:154312. Epub 2022 Jul 3.

Faculty of Chinese Medicine and State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, PR China; Zhuhai MUST Science and Technology Research Institute, Zhuhai, Guangdong, China.. Electronic address:

Background: Buyang Huanwu decoction (BYHWD) is a famous traditional Chinese formula that has been widely prescribed for sequelae of stroke in China. However, the efficacy and safety of BYHWD in treating sequelae of stroke have never been systematically evaluated.

Purpose: To evaluate the effectiveness and safety of BYHWD in the treatment of sequelae of stroke.

Study Design: A Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA)-compliant systematic review and meta-analysis of randomized clinical trials (RCTs).

Materials And Methods: A systematic review and meta-analysis was performed in accordance with the PRISMA guidelines. Five common electronic databases were searched for relevant RCTs from their inception until May 20, 2022. The Cochrane risk-of-bias tool was used to evaluate the methodological quality and the risk of bias of the included RCTs. Review Manager 5.4 was used to analyse all the data obtained. The clinical effective rate (CER) was the primary outcome, and National Institutes of Health Stroke Scale (NIHSS) and Fugl-Meyer Assessment (FMA) scores were the secondary outcomes. The Grading of Recommendations, Assessment, Development and Evaluations (GRADE) system was used to evaluate the quality of evidence for each outcome.

Results: Thirty-two clinical studies that recruited 2,527 eligible patients were included in this meta-analysis. The results of the meta-analysis suggested that compared with conventional treatment alone, the addition of BYHWD significantly improved the CER (RR = 1.24, 95% CI: 1.20-1.29, p < 0.00001), decreased the NIHSS score (MD = -5.42, 95% CI: -5.87-4.97, p < 0.00001), and increased the FMA score (MD = 17.28, 95% CI: 15.12-19.45, p < 0.00001). There were no reported adverse events in the included studies. Most results were robust, and the quality of evidence was moderate.

Conclusion: Our study is the first meta-analysis of RCTs evaluating the effects of BYHWD on sequelae of stroke. The addition of BYHWD to conventional treatment for sequelae of stroke significantly improved the CER and promoted neurological rehabilitation in patients, and there were no reported adverse events associated with this combination therapy. The findings of our study support the use of BYHWD as an adjunct treatment to conventional treatment in this clinical population. However, due to the limitations of the included clinical trials, high-quality clinical trials with longer follow-ups are needed to assess the long-term effectiveness and safety of BYHWD for treating the sequelae of stroke.
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http://dx.doi.org/10.1016/j.phymed.2022.154312DOI Listing
September 2022

MicroRNA214 expression inhibits HCC cell proliferation through PTK2b/ Pyk2.

Cell Mol Biol (Noisy-le-grand) 2022 May 22;68(1):20-25. Epub 2022 May 22.

Department of Integrated Traditional Chinese and Western Medicine, Wuhan Third Hospital, Wuhan, Hubei430060, China.

MicroRNAs (miRNAs/miRs) are crucial regulatory molecules that act as the most significantly downregulated microRNAs in hepatocellular carcinoma (HCC). PTK2b/Pyk2 is a non-receptor protein tyrosine kinase, which plays an important role in the development and metastasis of cancer. In this study, we explored the expression level and functional relationship between MicroRNA-214 (miR-214) and PTK2b/ Pyk2 in liver cancer cells. For this purpose, we analyzed the expression of miR-214 and PTK2b/Pyk2 in 38 cases of HCC and paired non-neoplastic tissue specimens using real-time PCR. MTT, cell cycle and construct recombinant plasmids analysis were used to explore the effects of miR-214 and PTK2b/Pyk2 on liver cancer cell proliferation. Results showed that the expression level of mir-214 in liver cancer tissues and liver cancer cell lines was significantly lower than that in normal tissues and cells, while the expression of PTK2b/Pyk2 was significantly increased. The overexpression of mir-214 or inhibition PTK2b/Pyk2 inhibited the proliferation of HCC cells. This research showed that mir-214 has an inhibitory effect on liver cancer through the expression of PTK2b/Pyk2.
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http://dx.doi.org/10.14715/cmb/2022.68.1.4DOI Listing
May 2022

Etiology distribution of abnormal uterine bleeding according to FIGO classification system: A combined study of ultrasound and histopathology.

J Obstet Gynaecol Res 2022 Jul 5;48(7):1913-1920. Epub 2022 Apr 5.

Department of Ultrasound, The Second Hospital of Shandong University, Jinan, China.

Aim: To classify the FIGO's PALM-COEIN etiology for abnormal uterine bleeding (AUB) patients according to the ultrasound and histopathology and to evaluate the etiology prevalence distribution for AUB patients.

Methods: A total of 1065 women with AUB in the Second Hospital of Shandong University between January 2019 and March 2021. The intrauterine histopathological and ultrasound results were reviewed retrospectively.PALM-COEIN etiology classification was performed according to histopathology and ultrasound. The classification system was stratified into nine basic categories: Polyp (AUB-P), adenomyosis (AUB-A), leiomyoma (AUB-L), malignancy and hyperplasia (AUB-M), coagulopathy (AUB-C), ovulatory disorders (AUB-O), endometrium (AUB-E), Iatrogenic(AUB-I), and not classified (AUB-N). The number of cases and composition ratio were also calculated.

Results: According to the FIGO's classification system, the PALM group (656,61.60%), which were defined as structural entities that can be measured visually with ultrasound and/or histopathology. 176 (16.53%) patients were classified as AUB-P,102 (9.58%) patients were classified as AUB-A, 487(45.73%) patients were classified as AUB-L, 31 (2.91%) patients were classified as AUB-M. The COEIN group (409,38.40%), which were defined as nonstructural entities that cannot be measured by ultrasound and/or histopathology. Nobody were classified as AUB-C in our group, and 383 (35.96%) patients were classified as AUB-O, 4 (0.38%) patients were classified as AUB-E,14 (1.31%) patients were classified as AUB-I,8 (0.75%) patients were classified as AUB-N.

Conclusion: In our study, AUB-L was the most common cause, followed by AUB-O, AUB-P, AUB-A, AUB-M, AUB-I, AUB-N, and AUB-E.PALM-COEIN etiology classification system played an important role in the epidemic and management standardization of AUB patients, provided an effective communication between physicians and researchers also. Ultrasonography was the preferred examination for AUB patients, especially with structural abnormalities.
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http://dx.doi.org/10.1111/jog.15226DOI Listing
July 2022

Long Short-Term Memory-Based Music Analysis System for Music Therapy.

Front Psychol 2022 14;13:928048. Epub 2022 Jun 14.

Hainan Hairui Zhong Chuang Technol Co. Ltd., Haikou, China.

Music can express people's thoughts and emotions. Music therapy is to stimulate and hypnotize the human brain by using various forms of music activities, such as listening, singing, playing and rhythm. With the empowerment of artificial intelligence, music therapy technology has made innovative development in the whole process of "diagnosis, treatment and evaluation." It is necessary to make use of the advantages of artificial intelligence technology to innovate music therapy methods, ensure the accuracy of treatment schemes, and provide more paths for the development of the medical field. This paper proposes an long short-term memory (LSTM)-based generation and classification algorithm for multi-voice music data. A Multi-Voice Music Generation system called MVMG based on the algorithm is developed. MVMG contains two main steps. At first, the music data are modeled to the MDPI and text sequence data by using an autoencoder model, including music features extraction and music clip representation. And then an LSTM-based music generation and classification model is developed for generating and analyzing music in specific treatment scenario. MVMG is evaluated based on the datasets collected by us: the single-melody MIDI files and the Chinese classical music dataset. The experiment shows that the highest accuracy of the autoencoder-based feature extractor can achieve 95.3%. And the average F1-score of LSTM is 95.68%, which is much higher than the DNN-based classification model.
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http://dx.doi.org/10.3389/fpsyg.2022.928048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237431PMC
June 2022

Molecular cloning, expression and appetite regulation function of adiponectin in Siberian sturgeon (Acipenser baerii).

Int J Biol Macromol 2022 Aug 15;214:360-369. Epub 2022 Jun 15.

Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211 # Huimin Road, Chengdu 610000, China. Electronic address:

Adiponectin (AdipoQ) as an adipocytokine has the potential to regulate feeding behavior, but the information about adipoq in fish is limited. In this study, Siberian sturgeon adiponectin (Ssadipoq) gene was cloned encoding 264 amino acids. The amino acid identity of SsAdipoQ was low compared with that of mammals, birds, amphibians and teleost fishes. The expression of Ssadipoq in the hypothalamus was significantly decreased at 1 h and 3 h post feeding, and increased after 15-day fasting. The mature domain of AdipoQ (fAd) was inserted into expression vector pET32a and successfully expressed in Escherichia coli BL21 (DE3) after stimulated by isopropyl-β-d-thiogalactoside. Food intake at 1 h and 3 h post treatment with SsfAd protein decreased significantly (P < 0.05). The mRNA expression of pyy and cck in the valvula intestine was promoted and hypothalamic npy, agrp and pomc mRNA expression were inhibited after treatment with SsfAd protein. Furthermore, hypothalamic ampk subunits expression was associated with peripheral SsfAd treatment. In summary, present study indicate that SsfAd plays an important role in the regulation of food intake and appetite signals in Siberian sturgeon, which provides a basis for further study application of prokaryotic AdipoQ in feeding behavior regulation.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.06.097DOI Listing
August 2022

Label-free detection and enumeration of rare circulating tumor cells by bright-field image cytometry and multi-frame image correlation analysis.

Lab Chip 2022 Jun 16. Epub 2022 Jun 16.

School of Information Engineering, Zhengzhou University, Zhengzhou 450001, China.

Identification and enumeration of circulating tumor cells (CTCs) in peripheral blood are proved to correlate with the progress of metastatic cancer and can provide valuable information for diagnosis and monitoring of cancer. Here, we introduce a bright-field image cytometry (BFIC) technique, assisted by a multi-frame image correlation (MFIC) algorithm, as a label-free approach for tumor cell detection in peripheral blood. For this method, images of flowing cells in a wide channel were continuously recorded and cell types were determined simultaneously using a deep neural network of YOLO-V4 with an average precision (AP) of 98.63%, 99.04%, and 98.95% for cancer cell lines HT29, A549, and KYSE30, respectively. The use of the wide microfluidic channel (400 μm width) allowed for a high throughput of 50 000 cells per min without clogging. Then erroneous or missed cell classifications caused by imaging angle differences or accidental misinterpretations in single frames were corrected by the multi-frame correlation analysis. This further improved the AP to 99.40%, 99.52%, and 99.47% for HT29, A549, and KYSE30, respectively. Meanwhile, cell counting was also accomplished in this dynamic process. Moreover, our imaging cytometry method can readily detect as few as 10 tumor cells from 100 000 white blood cells and was unaffected by the EMT process. Furthermore, CTCs from 8 advanced-stage cancer clinical samples were also successfully detected, while none for 6 healthy control subjects. Although this method is implemented for CTCs, it can also be used for the detection of other rare cells.
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http://dx.doi.org/10.1039/d2lc00190jDOI Listing
June 2022

Association between plasma Vitamin B5 levels and all-cause mortality: A nested case-control study.

J Clin Hypertens (Greenwich) 2022 Jul 14;24(7):945-954. Epub 2022 Jun 14.

Department of Clinical Laboratory, the First Affiliated Hospital of Kunming Medical University, Kunming, China.

We aimed to evaluate the prospective association of vitamin B5 with all-cause mortality and explore its potential modifiers in Chinese adults with hypertension. A nested, case-control study was conducted in the China Stroke Primary Prevention Trial, including 505 deaths of all causes and 505 matched controls. The median follow-up duration was 4.5 years. The primary outcome measure in this investigation was all-cause mortality, which encompassed deaths for any reason. The mean plasma vitamin B5 concentration for cases (43.7 ng/mL) was higher than that in controls (40.9 ng/mL) (p = .001). When vitamin B5 was further assessed as quintiles, compared with the reference group (Q1: < 33.0 ng/mL), the risk of all-cause mortality increased by 29% (OR = 1.29, 95% CI: 0.83-2.01) in Q2, 22% (OR = 1.22, 95% CI: 0.77-1.94) in Q3, 62% (OR = 1.62, 95% CI: 1.00-2.62) in Q4, and 77% (OR = 1.77, 95% CI: 1.06-2.95) in Q5. The trend test was significant (p = .022). When Q4-Q5 were combined, a significant 41% increment (OR = 1.41, 95% CI: 1.03-1.95) in all-cause death risk was found compared with Q1-Q3. The adverse effects were more pronounced in those with normal folate levels (p-interaction = .019) and older people (p-interaction = .037). This study suggests that higher baseline levels of plasma vitamin B5 are a risk factor for all-cause mortality among Chinese patients with hypertension, especially among older adults and those with adequate folate levels. The findings, if confirmed, may inform novel clinical and nutritional guidelines and interventions to optimize vitamin B5 levels.
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http://dx.doi.org/10.1111/jch.14516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278592PMC
July 2022

Corrigendum to "Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration" [Carbohydrate Polymers Volume 290, 2022, 119499].

Carbohydr Polym 2022 Sep 19;291:119613. Epub 2022 May 19.

Department of Neurosurgery, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, PR China.

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http://dx.doi.org/10.1016/j.carbpol.2022.119613DOI Listing
September 2022

One-step fabrication of nitrogen-rich linear porous organic polymer-based micron-sized sphere for selective enrichment of glycopeptides.

Anal Chim Acta 2022 Jul 25;1215:339988. Epub 2022 May 25.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

In this work, nitrogen-rich linear porous organic polymers (POPs) were designed to improve glycopeptides enrichment efficiency in HILIC. Two kinds of novel POPs (CH-pPAL and CH-mPAL) were one-step prepared via the Schiff-base condensation reaction and intermolecular hydrogen-bonded using a flexible amine monomer (carbohydrazide, CH) to react with two aldehyde monomers, p-phthalaldehyde (pPAL) and m-phthalaldehyde (mPAL), respectively. The specific surface area of CH-pPAL and CH-mPAL was 238 and 232 m g, respectively. Based on the hydrogen-bond interaction, several narrow peaks existed in the PXRD patterns. Particularly, micron-sized spherical CH-pPAL possessed a honeycomb-like orderly tunnel structure. The contact angle of CH-pPAL and CH-mPAL severally was 35.4° and 28.8°, respectively, indicating excellent hydrophilicity of as-synthesized POPs. They both demonstrated high sensitivity and high adsorption capacity in glycopeptides enrichment. The 851 and 794 unique glycopeptides derived from 170 and 200 N-glycosylated proteins were identified after the enrichment by CH-pPAL and CH-mPAL within three independent replicates of proteins extracted from human serum, respectively. Therefore, these CH-based hydrophilic POP materials would be applied in the enrichment and identification of low-abundance N-linked glycopeptides from complicated biological samples.
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http://dx.doi.org/10.1016/j.aca.2022.339988DOI Listing
July 2022

The Bufei Jianpi Formula Improves Mucosal Immune Function by Remodeling Gut Microbiota Through the SCFAs/GPR43/NLRP3 Pathway in Chronic Obstructive Pulmonary Disease Rats.

Int J Chron Obstruct Pulmon Dis 2022 1;17:1285-1298. Epub 2022 Jun 1.

Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Henan University of Chinese Medicine, Zhengzhou, People's Republic of China.

Purpose: Bufei Jianpi formula (BJF), a traditional Chinese medicine, is an effective and safe therapeutic formula for chronic obstructive pulmonary disease (COPD). BJF treatment is known to reduce the incidence of loose stools in rats with COPD. It is unclear whether BJF regulates gut microbiota. This study examined whether BJF improved mucosal immune function by remodeling the gut microbiota and modulating metabolites in COPD rats.

Methods: Sixty Sprague Dawley (SD) rats were randomized into control, model, BJF, aminophylline (APL), and probiotics (PBT) groups. The stable COPD rat model was duplicated using repeated cigarette smoke inhalation and lipopolysaccharide (LPS) injection. Normal saline, BJF, APL, or PBT were intragastrically administered from weeks eight to twelve, and then the rats were sacrificed at week thirteen. Lung and colon tissues were removed; feces were collected. Pulmonary function, histopathology, levels of inflammatory factors, and activation of NF-κB in the lung tissues were evaluated. Gut microbiota were analyzed using 16S rRNA gene sequencing; fecal short-chain fatty acid (SCFA) concentrations were determined using gas chromatography/mass spectrometry. Mucosal immune response-related genes and proteins were determined using quantitative polymerase chain reaction and Western blotting.

Results: BJF improved pulmonary function and reduced lung inflammation. Further, BJF treatment altered the gut microbiota composition and significantly increased the abundance of Firmicutes and the ratio of Firmicutes to Bacteroides, raising SCFA levels, including acetate, butyrate, and propionate levels. However, the abundance of Bacteroidetes, Proteobacteria, Spirochaetes, Clostridiaceae, and Treponema decreased after BJF administration. BJF decreased the gene and protein expression of NLRP3, Caspase-1, IL-8, and IL-1β, and increased GPR43 expression.

Conclusion: Overall, BJF administration improved mucosal immune function by remodeling the gut microbiota and suppressing the SCFAs/GPR43/NLRP3 pathway in COPD rats. This study provides evidence for the mechanisms underlying BJF-induced improvements in COPD and supports clinical application of BJF.
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http://dx.doi.org/10.2147/COPD.S359428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167601PMC
June 2022

Targeted inhibition of STAT3 induces immunogenic cell death of hepatocellular carcinoma cells via glycolysis.

Mol Oncol 2022 Aug 27;16(15):2861-2880. Epub 2022 Jun 27.

Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, Shandong University, Jinan, China.

In hepatocellular carcinoma (HCC), the signal transducer and activator of transcription 3 (STAT3) is present in an overactive state that is closely related to tumour development and immune escape. STAT3 inhibition reshapes the tumour immune microenvironment, but the underlying mechanisms have not been fully clarified. We found that STAT3 inhibition could induce immunogenic cell death (ICD) of HCC cells via translocation of the "eat me" molecule calreticulin to the cell surface and a significant reduction in the expression of the "don't eat me" molecule leucocyte surface antigen CD47. STAT3 inhibition promoted dendritic cell (DC) activation and enhanced the recognition and phagocytosis of HCC cells by macrophages. Furthermore, STAT3 inhibition prevented the expression of key glycolytic enzymes, facilitating the induction of ICD in HCC. Interestingly, STAT3 directly regulated the transcription of CD47 and solute carrier family 2 member 1 (SLC2A1; also known as GLUT1). In subcutaneous and orthotopic transplantation mouse tumour models, the STAT3 inhibitor napabucasin prevented tumour growth and induced the expression of calreticulin and the protein disulfide isomerase family A member 3 (PDIA3; also known as ERp57) but suppressed that of CD47 and GLUT1. Meanwhile, the amount of tumour-infiltrated DCs and macrophages increased, along with the expression of costimulatory molecules. More CD4 and CD8 T cells accumulated in tumour tissues, and CD8 T cells had lower expression of checkpoint molecules such as lymphocyte activation gene 3 protein (LAG-3) and programmed cell death protein 1 (PD-1). Significantly, the antitumour immune memory response was induced by treatment targeting STAT3. These findings provide a new mechanism for targeting STAT3-induced ICD in HCC, and confirms STAT3 as a potential target for the treatment of HCC via reshaping the tumour immune microenvironment.
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http://dx.doi.org/10.1002/1878-0261.13263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348600PMC
August 2022

Neuromedin U regulates food intake of Siberian sturgeon through the modulation of central and peripheral appetite factors.

Br J Nutr 2022 Jun 6:1-15. Epub 2022 Jun 6.

Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, Sichuan, People's Republic of China.

Neuromedin U (NMU) has a critical function on the regulation of food intake in mammals, while the information is little in teleost. To investigate the function of NMU on appetite regulation of Siberian sturgeon (), this study first cloned cDNA sequence that encoded 154 amino acids including NMU-25 peptide. Besides, the results showed that mRNA was widely distributed in various tissues especially in the hypothalamus and telencephalon. The results of nutritional status (pre-feeding and post-feeding, fasting and re-feeding) experiments showed that mRNA expression was significantly decreased at 1 and 3 h after feeding in different brain regions. Similarly, after feeding, the expression of significantly decreased in peripheral tissues. Moreover, expression in the hypothalamus was significantly increased after fasting 1 d, but decreased after fasting 17 d, which was significantly reversed after re-feeding. However, other brain regions like telencephalon and peripheral tissues like oesophagus, intestinum valvula and liver have different change patterns. Further study showed that acute and injection of NMU and chronic injection of NMU significantly reduced the food intake in a dose-dependent mode. In addition, the expressions of several critical appetite factors (, , , , , , , and ) were significantly affected by acute NMU-25 administration in the hypothalamus, intestinum valvula and liver. These results indicate that NMU-25 has the anorexigenic function on food intake by affecting different appetite factors in Siberian sturgeon, which provides a foundation for further exploring the appetite regulation networks in fish.
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http://dx.doi.org/10.1017/S0007114522001696DOI Listing
June 2022

Construction of Machine Learning Models to Predict Changes in Immune Function Using Clinical Monitoring Indices in HIV/AIDS Patients After 9.9-Years of Antiretroviral Therapy in Yunnan, China.

Front Cell Infect Microbiol 2022 12;12:867737. Epub 2022 May 12.

Department of Laboratory Medicine, First Affiliated Hospital of Kunming Medical University, Kunming, China.

Objective: To investigate trends in clinical monitoring indices in HIV/AIDS patients receiving antiretroviral therapy (ART) at baseline and after treatment in Yunnan Province, China and to provide the basis for guiding clinical treatment to obtain superior clinical outcomes.

Methods: A total of 96 HIV/AIDS patients who had started and persisted in highly active ART treatment from September 2009 to September 2019 were selected. Of these, 54 had a CD4 cell count < 200 cells/μl while 42 had a CD4 cell count ≥ 200 cells/μl. Routine blood tests, liver and renal function, and lipid levels were measured before and 3, 6, 9, and 12 months after treatment. Lymphocyte subset counts and viral load were measured once per year, and recorded for analysis and evaluation. Three machine learning models (support vector machine [SVM], random forest [RF], and multi-layer perceptron [MLP]) were constructed that used the clinical indicators above as parameters. Baseline and follow-up results of routine blood and organ function tests were used to analyze and predict CD4 T cell data after treatment during long-term follow-up. Predictions of the three models were preliminarily evaluated.

Results: There were no statistical differences in gender, age, or HIV transmission route in either patient group. Married individuals were substantially more likely to have <200 CD4 cells/μl. There was a strong positive correlation between ALT and AST (r = 0.587) and a positive correlation between CD4 cell count and platelet count (r = 0.347). Platelet count was negatively correlated with ALT (r = -0.229), AST (r = -0.251), and positively correlated with WBCs (r = 0.280). Compared with the CD4 cell count < 200 cells/μl group, all three machine learning models exhibited a better predictive capability than for patients with a CD4 cell count ≥ 200 cells/μl. Of all indicators, the three models best predicted the CD4/CD8 ratio, with results that were highly consistent. In patients with a CD4 cell count < 200 cells/μl, the SVM model had the best performance for predicting the CD4/CD8 ratio, while the CD4/CD8 ratio was best predicted by the RF model in patients with a CD4 cell count ≥ 200 cells/μl.

Conclusion: By the incorporation of clinical indicators in SVM, RF, and MLP machine learning models, the immune function and recuperation of HIV/AIDS patients can be predicted and evaluated, thereby better guiding clinical treatment.
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http://dx.doi.org/10.3389/fcimb.2022.867737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134793PMC
May 2022

The relation between red blood cell distribution width and coronary atherosclerotic plaque vulnerability detected by intracoronary optical coherence tomography.

Curr Vasc Pharmacol 2022 May 27. Epub 2022 May 27.

Department of Cardiology, 12th ward, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Disease, Beijing Key Laboratory of Precision Medicine of Coronary Atherosclerotic Disease, Clinical center for coronary heart disease, Capital Medical University,Beijing 100029, China.

Background: A higher red blood cell distribution width (RDW) predicts major adverse cardiac events in patients with coronary artery disease (CAD). However, there are only few studies regarding the relationship between RDW and vulnerable plaques. Thus, the purpose of present study is to retrospectively explore the predictive value of the association between RDW and plaque vulnerability assessed by optical coherence tomography (OCT) in patients with cardiovascular (CV) diseases.

Methods: This study included 35 patients with stable angina pectoris (SAP) and 70 patients with acute coronary syndrome (ACS). We documented clinical features as well as peripheral RDW. Plaque vulnerability was determined by OCT. We defined thin-cap fibroatheroma (TCFA) as a lipid-rich plaque (fibrous cap <65 μm thick).

Results: Plaque rupture were detected more frequently in patients with ACS compared with patients with SAP (62.9 vs 2.9%, p<0.001 and the corresponding TCFA was 50.69±15.68 vs 80.03±21.60 μm, p<0.001, respectively). A higher RDW was found in patients with ACS than in patients with SAP (p<0.001). A cut-off value of RDW >13.85% could detect ruptured plaque with a sensitivity of 72.3% and a specificity of 62%.

Conclusions: TCFA and plaque rupture were detected more frequently in patients with ACS compared with SAP. Elevated RDW were positively the predictive value of the association between plaque vulnerability.
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http://dx.doi.org/10.2174/1570161120666220527093551DOI Listing
May 2022

Effect of bronchofiberscopic lavage with budesonide suspension on refractory mycoplasma pneumoniae pneumonia.

Pak J Med Sci 2022 Mar-Apr;38(4Part-II):922-927

Xiaohua Gou Pulmonary and Critical Care Medicine 2 Department, Baoding First Central Hospital, Baoding071000, Hebei, China.

Objectives: To investigate the clinical efficacy of budesonide suspension through bronchofiberscopic lavage in the treatment of refractory mycoplasma pneumoniae pneumonia.(RMPP).

Methods: A total of 108 children with refractory mycoplasma pneumoniae pneumonia from June 2018 to October 2020 were randomly divided into two groups: the study group and the control group, with 54 cases in each group. Children in the control group were treated with anti-infective, antitussive, antipyretic routine symptomatic treatment and nebulization of budesonide suspension, while children in the study group were treated with bronchofiberscopic lavage with Budesonide suspension along with standard treatment as in control group. The clinical efficacy, changes in blood gas analysis indexes, levels of inflammatory mediators, time for improvement of clinical symptoms and the incidence of adverse reactions were compared between the two groups.

Results: The efficacy of treatment in the study group was 85.19%, which was higher than 66.67% in the control group (P<0.05). The antipyretic time, antitussive time, lung rales disappearance time and length of hospital stay in the study group were shorter than those in the control group (P<0.05). Moreover, the levels of arterial oxygen partial pressure (PaO) in the study group were higher than those in the control group, while the levels of partial pressure of carbon dioxide (PaCO) and arterial oxygen saturation (SaO) in the study group were lower than those in the control group (P<0.05). The levels of inflammatory mediators interleukin-6 (IL-6), interleukin-4 (IL-4), and interferon-γ (IFN-γ) in the study group were lower than those in the control group (P<0.05), and no significant difference was found in the comparison of the incidence of adverse reactions between the two groups (P>0.05).

Conclusions: Budesonide suspension combined with bronchofiberscopic lavage with Budesonide suspension is a safe treatment regimen that can significantly improve the clinical outcome in children with RMPP. With such a combined treatment regimen, the clinical symptoms of children can be ameliorated, the ventilation function can be enhanced, and the level of inflammatory mediators will be reduced.
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http://dx.doi.org/10.12669/pjms.38.4.4835DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121973PMC
July 2021

Static test research on steel bridge deck pavement structures paved by high-content hybrid fibre polymer concrete.

Sci Rep 2022 05 25;12(1):8796. Epub 2022 May 25.

School of Civil Engineering, Chongqing Jiaotong University, Chongqing, 400074, China.

Steel bridge deck pavement has always been a key and difficult point in the construction of long-span bridges. In practical engineering, common paving material is asphalt, and serious damage is caused on the pavement layer in the early stage. In this study, a high-performance high-content hybrid fibre polymer concrete was used as the paving material. A test was conducted on the small beam of the composite structure formed by the pavement layer and steel plate, and a positive/negative bending moment test was conducted to analyse the stiffness and bearing capacity of the composite structure. As revealed in the research results, the flexural and tensile stiffness of the structure and the bearing capacity of the composite structure showed superior performance, increased significantly with the pavement thickness, but increased slowly after the pavement thickness exceeded 80 mm and the increase in thickness contributed little to the bearing capacity. Under the simulated action of a positive/negative bending moment, the pavement layer still exhibited certain ductile failure features when the structure was bearing an ultimate load. This proves that high-content hybrid fibre polymer concrete exhibits suitable mechanical properties for steel bridge deck pavement.
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http://dx.doi.org/10.1038/s41598-022-12987-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132993PMC
May 2022

Correction: Childhood trauma and current depression among Chinese university students: a moderated mediation model of cognitive emotion regulation strategies and neuroticism.

BMC Psychiatry 2022 May 16;22(1):340. Epub 2022 May 16.

Department of Clinical Psychology, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China.

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http://dx.doi.org/10.1186/s12888-022-03960-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112540PMC
May 2022

Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration.

Carbohydr Polym 2022 Aug 19;290:119499. Epub 2022 Apr 19.

Department of Neurosurgery, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, PR China.

As an important transcription factor, c-Jun could upregulate growth factors expression in Schwann cells (SCs). Arginine-Glycine-Aspartate (RGD)-functionalized chitosan-graft-polyethyleneimine (RCP) gene vectors were prepared through the maleic anhydride & the carbodiimide methods, and electrostatically bound with c-Jun plasmids (pJUN), finally loaded on poly-L-lactic acid/silk fibroin parallel fiber films to fabricate nerve scaffold (RCP/[email protected]), which could locally deliver c-Jun plasmids into SCs via the mediation of RGD peptides, and upregulate the expression of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in SCs. After the scaffold was bridged in sciatic nerve defect, the delivery of c-Jun plasmids from RCP/[email protected] facilitated SCs to sustain the expressions of NGF, BDNF and vascular endothelial growth factor in the injury field, promoting myelination, axonal growth and microvascular generation and nerve regeneration, muscle reinnervation and functional recovery. These results suggested that RCP/[email protected], as an effective gene delivery platform, could provide a local gene therapy to improve nerve regeneration.
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http://dx.doi.org/10.1016/j.carbpol.2022.119499DOI Listing
August 2022

Anti-Apoptosis of Podocytes and Pro-Apoptosis of Mesangial Cells for Telmisartan in Alleviating Diabetic Kidney Injury.

Front Pharmacol 2022 19;13:876469. Epub 2022 Apr 19.

Department of Pharmacy, Shanghai East Hospital, Tongji University, Shanghai, China.

Podocytes damage and mesangial cells expansion are two important pathological manifestations of glomerular injury in early diabetes. Telmisartan, as an angiotensin type 1 (AT) receptor inhibitor, could improve advanced glycation end (AGE) products or angiotensin Ⅱ (Ang Ⅱ)-induced podocytes injury including detachment or apoptosis. In this current paper, we first confirmed the protective effect of telmisartan on early diabetic kidney injury in type 1 diabetic rats. Telmisartan reduced the loss of podocin and inhibited the expression of α-SMA, reflecting its protective effect on podocyte injury and mesangial proliferation, respectively. More interestingly we observed an opposite effect of telmisartan on the cell viability and apoptosis of podocytes and mesangial cells in a high-glucose environment . The anti-apoptotic effect of telmisartan on podocytes might be related to its inhibition of swiprosin-1 (a protein can mediate high glucose-induced podocyte apoptosis) expression. While telmisartan induced a high expression of PPARγ in mesangial cells, and GW9662 (a PPARγ antagonist) partially inhibited telmisartan-induced apoptosis and reduced viability of mesangial cells. In addition, high glucose-induced PKCβ1/TGFβ1 expression in mesangial cells could be blocked by telmisartan. These data provide a more precise cellular mechanism for revealing the protective effect of telmisartan in diabetic kidney injury.
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http://dx.doi.org/10.3389/fphar.2022.876469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061946PMC
April 2022

The insulin gene as an energy homeostasis biomarker in Yangtze sturgeon (Acipenser dabryanus).

Fish Physiol Biochem 2022 Jun 3;48(3):693-705. Epub 2022 May 3.

College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, 611130, China.

Insulin plays an important role in maintaining energy homeostasis and has the potential to be an indicator of energy homeostasis in the Yangtze sturgeon, Acipenser dabryanus. In this study, the Yangtze sturgeon insulin (Adinsulin) was cloned and characterized. To evaluate the possibility of insulin as an energy state assessment indicator, quantification real-time PCR (qRT-PCR) was used to evaluate expression changes in different tissues (the whole brain, esophagus, cardiac stomach, pyloric stomach, pyloric caeca, duodenum, valvula intestine, rectum, liver, pancreas, spleen, kidney, heart, muscle, gill and eye) from 6 fish (average weight 325.7 ± 22.3 g) and in three experiments including postprandial, fasting and re-feeding, and glucose tolerance treatment in which fish were divided into two groups including a group that administered a glucose solution (1 ul/g body weight) and another group that administered sterile water as control. In these three experiments, 6 fish were sampled, respectively, then been used to evaluate expression changes of insulin. All fish in feeding groups were fed in tanks (60.0 cm × 50.0 cm × 40.0 cm) with a commercial diet (crude protein ≥ 40%, crude fat ≥ 12%, coarse fiber ≤ 6%, crude ash ≤ 18%; TONGWEI CO., LTD, China) once a day at 16:00. The result showed that Adinsulin was highly expressed in the pancreas, which was the basis for the next experiment to use the pancreas as the test target. Adinsulin expression significantly increased 1 h after feeding and decreased rapidly after 3 h of feeding, but it was still significantly higher than that of the group without feeding (P < 0.01). Compared to the feeding group, the expression of Adinsulin was significantly reduced in the fasting group of 3 days (P < 0.01), 6 days (P < 0.01), 10 days (P < 0.05), 11 days (P < 0.05) and 13 days (P < 0.01) and was no significant difference in re-feeding for 1st day, 2nd day and 4th day, but there was difference between re-feeding group and fasting group. After glucose tolerance treatment, serum glucose levels increased significantly (P < 0.05), accompanied by a significant increase (P < 0.001) in insulin expression. This study result shows that insulin has the capacity to measure the energy homeostasis of Yangtze sturgeon. Further development of detection methods for sturgeon plasma or serum insulin will avoid slaughtering animals and is more practical in energy homeostasis assessment.
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http://dx.doi.org/10.1007/s10695-022-01079-zDOI Listing
June 2022
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