Publications by authors named "Xujun Chen"

32 Publications

Comparison of mitochondrial genomes from multi-, Bi-, and uninucleate .

Mitochondrial DNA B Resour 2021 Feb 9;6(2):472-474. Epub 2021 Feb 9.

Key Laboratory of Pest Monitoring and Green Management, MOA, Department of Plant Pathology, China Agricultural University, Beijing, China.

Six circular mitochondrial genomes of multi-, bi-, and uninucleate isolates were assembled and found that all the genomes contain 14 conserved protein-coding genes, one ribosomal protein (rps3), and 23 tRNA in the same order. The mitogenome sizes of uninucleate isolates were relatively smaller than binucleate and multinucleate stains. The size variations between uninucleate and multinucleate isolates were from both intergenic and intronic regions, whereas the differences between uninucleate and binucleate isolates were predominantly from intergenic regions. The phylogenetic analysis revealed that strains of the same nucleate types had a closer relationship.
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http://dx.doi.org/10.1080/23802359.2021.1872430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889275PMC
February 2021

Evolutionary and genomic comparisons of hybrid uninucleate and nonhybrid Rhizoctonia fungi.

Commun Biol 2021 Feb 15;4(1):201. Epub 2021 Feb 15.

Key Laboratory of Pest Monitoring and Green Management, MOA; Joint Laboratory for International Cooperation in Crop Molecular Breeding; Department of Plant Pathology, China Agricultural University, Beijing, China.

The basidiomycetous fungal genus, Rhizoctonia, can cause severe damage to many plants and is composed of multinucleate, binucleate, and uninucleate species differing in pathogenicity. Here we generated chromosome-scale genome assemblies of the three nuclear types of Rhizoctonia isolates. The genomic comparisons revealed that the uninucleate JN strain likely arose by somatic hybridization of two binucleate isolates, and maintained a diploid nucleus. Homeolog gene pairs in the JN genome have experienced both decelerated or accelerated evolution. Homeolog expression dominance occurred between JN subgenomes, in which differentially expressed genes show potentially less evolutionary constraint than the genes without. Analysis of mating-type genes suggested that Rhizoctonia maintains the ancestral tetrapolarity of the Basidiomycota. Long terminal repeat-retrotransposons displayed a reciprocal correlation with the chromosomal GC content in the three chromosome-scale genomes. The more aggressive multinucleate XN strain had more genes encoding enzymes for host cell wall decomposition. These findings demonstrate some evolutionary changes of a recently derived hybrid and in multiple nuclear types of Rhizoctonia.
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http://dx.doi.org/10.1038/s42003-021-01724-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884421PMC
February 2021

Optimization of Fermentation Process for Selenium Enrichment as Organic Selenium Source.

Front Nutr 2020 5;7:543873. Epub 2020 Nov 5.

National R&D Center for Se-rich Agricultural Products Processing, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.

Selenium is an essential trace element and micronutrient for human health. Application of organic selenium in plants and microorganisms as trace element supplement is attracting more and more attention. In this study, , an important probiotic, was used for selenium enrichment with sodium selenite as selenium source. The growth curve of was investigated, and 150 μg/ml was selected as the concentration of selenium for fermentation. With application of response surface methodology, the optimal fermentation conditions were obtained as follows: inoculation quantity of 7%, culture temperature of 33°C, and shaking speed of 170 rpm, leading to the maximal selenium conversion ratio of 94.3 ± 0.2%. Field emission scanning electron microscope and energy dispersive spectrometry evidenced that inorganic selenium had been successfully transformed. This study may contribute to get a strain with high Se conversion ratio, so as to extract organic selenium in the form of selenoprotein to be used for further application.
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http://dx.doi.org/10.3389/fnut.2020.543873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674919PMC
November 2020

Ketamine affects the integration of developmentally generated granule neurons in the adult stage.

BMC Neurosci 2019 12 18;20(1):60. Epub 2019 Dec 18.

Department of Anesthesiology, First Affiliated Hospital With Nanjing Medical University, Guangzhou Road 300, Nanjing, 210029, Jiangsu, People's Republic of China.

Background: Ketamine has been reported to cause neonatal neurotoxicity in a variety of developing animal models. Various studies have been conducted to study the mechanism of neurotoxicity for general anesthetic use during the neonatal period. Previous experiments have suggested that developmentally generated granule neurons in the hippocampus dentate gyrus (DG) supported hippocampus-dependent memory. Therefore, this study aimed to investigate whether ketamine affects the functional integration of developmentally generated granule neurons in the DG. For this purpose,the postnatal day 7 (PND-7) Sprague-Dawley (SD) rats were divided into the control group and the ketamine group (rats who received 4 injections of 40 mg/kg ketamine at 1 h intervals). To label dividing cells, BrdU was administered for three consecutive days after the ketamine exposure; NeuN+/BrdU+cells were observed by using immunofluorescence. To evaluate the developmentally generated granule neurons that support hippocampus-dependent memory, spatial reference memory was tested by using Morris Water Maze at 3 months old, after which the immunofluorescence was used to detect c-Fos expression in the NeuN/BrdU cells. The expression of caspase-3 was measured by western blot to detect the apoptosis in the hippocampal DG.

Results: The present results showed that the neonatal ketamine exposure did not influence the survival rate of developmentally generated granule neurons at 2 and 3 months old, but ketamine interfered with the integration of these neurons into the hippocampal DG neural circuits and caused a deficit in hippocampal-dependent spatial reference memory tasks.

Conclusions: In summary, these findings may promote more studies to investigate the neurotoxicity of ketamine in the developing brain.
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http://dx.doi.org/10.1186/s12868-019-0542-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921590PMC
December 2019

Comparison of Baumgaertner and Chang reduction quality criteria for the assessment of trochanteric fractures.

Bone Joint Res 2019 Oct 2;8(10):502-508. Epub 2019 Nov 2.

Department of Orthopaedics, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China.

Objectives: Different criteria for assessing the reduction quality of trochanteric fractures have been reported. The Baumgaertner reduction quality criteria (BRQC) are relatively common and the Chang reduction quality criteria (CRQC) are relatively new. The objectives of the current study were to compare the reliability of the BRQC and CRQC in predicting mechanical complications and to investigate the clinical implications of the CRQC.

Methods: A total of 168 patients were assessed in a retrospective observational study. Clinical information including age, sex, fracture side, American Society of Anesthesiologists (ASA) classification, tip-apex distance (TAD), fracture classification, reduction quality, blade position, BRQC, CRQC, bone quality, and the occurrence of mechanical complications were used in the statistical analysis.

Results: A total of 127 patients were included in the full analysis, and mechanical complications were observed in 26 patients. The TAD, blade position, BRQC and CRQC were significantly associated with mechanical complications in the univariate analysis. Only the TAD (p = 0.025) and the CRQC (p < 0.001) showed significant results in the multivariate analysis. In the comparison of the receiver operating characteristic curves, the CRQC also performed better than the BRQC.

Conclusion: The CRQC are reliable in predicting mechanical complications and are more reliable than the BRQC. Future studies could use the CRQC to assess fracture reduction quality. Intraoperatively, the surgeon should refer to the CRQC to achieve good reduction in trochanteric fractures.: 2019;8:502-508.
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http://dx.doi.org/10.1302/2046-3758.810.BJR-2019-0032.R1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825041PMC
October 2019

Exosomal MMP2 derived from mature osteoblasts promotes angiogenesis of endothelial cells via VEGF/Erk1/2 signaling pathway.

Exp Cell Res 2019 10 29;383(2):111541. Epub 2019 Jul 29.

Department of Orthopedics, The Fifth People's Hospital of Shanghai, Fudan University, China; Department of Orthopedics, Huashan Hospital, Fudan University, China. Electronic address:

The skeletal system is a dynamic organ that continuously undergoes coupled trabeculae and blood vessels remodeling, indicating the possible existence of molecular crosstalk between endothelial and osteoblastic cells. Since the cross-talk between bone-forming osteoblasts (OBs) and vessel-forming endothelial cells (ECs) have progressively gained investigators' attention, few studies focused on the regulatory function of extracellular vesicles derived from OBs on ECs. In this study, the effect of the exosomes derived from mature osteoblasts (MOBs) on the ECs was investigated. Firstly, exosomes derived from mature osteoblasts (MOB-Exos) were isolated and identified by NanoSight light scatter technology, electron microscopy and Western bolting. Fluorescent labeling of MOB-Exos revealed its internalization by ECs. RNA interference technique was used to knock down matrix metalloproteinase-2 (MMP2) in MOB-Exos. Then ECs were co-cultured with MOB-Exos and MMP2 knockdown MOB-Exos. Wound healing migration assay, transwell migration assay, CCK-8 assay and tube formation assay of ECs were conducted to determine the angiogenic capability of ECs. Then the VEGF/Erk1/2 pathway markers were detected by Western blot. Our results showed that MOB-Exos could promote the proliferation, migration and tube formation of ECs. Meanwhile, the promoted angiogenetic capacities of ECs were impaired when MMP2 in MOB-Exos was knocked down. In addition, immunoblotting indicated that MOB-Exos could promote the activation of the VEGF/Erk1/2 pathway of ECs; whereas the activation of the VEGF/Erk1/2 pathway was attenuated when the ECs were co-cultured with the MMP2 knockdown MOB-Exos. In conclusion, the MMP-2 existing in exosomes derived from MOBs could promote the angiogenesis of ECs in vitro, which might be realized through VEGF/Erk1/2 signaling pathway.
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http://dx.doi.org/10.1016/j.yexcr.2019.111541DOI Listing
October 2019

The Role of Continuous Cerebrospinal Fluid Pulsation Stress in the Remodeling of Artificial Vertebral Laminae: A Comparison Experiment.

Tissue Eng Part A 2019 02 15;25(3-4):203-213. Epub 2018 Sep 15.

Department of Orthopedics, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, People's Republic of China.

Impact Statement: In this study, we designed a comparison study to investigate the effect of continuous cerebrospinal fluid pulsation (CSFP) stress on the remodeling of artificial vertebral laminae, and we found that the continuous CSFP stress played an important role in the physiological reconstruction of artificial vertebral laminae by promoting the remodeling abilities of tissue-engineered laminae (TEL). It deepens our understanding of the in vivo development of TEL, and the impact of biomechanical stimuli on the osteogenesis and remodeling.
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http://dx.doi.org/10.1089/ten.TEA.2018.0100DOI Listing
February 2019

The rice terpene synthase gene OsTPS19 functions as an (S)-limonene synthase in planta, and its overexpression leads to enhanced resistance to the blast fungus Magnaporthe oryzae.

Plant Biotechnol J 2018 10 6;16(10):1778-1787. Epub 2018 Apr 6.

Department of Plant Sciences, University of Tennessee, Knoxville, TN, USA.

Rice blast disease, caused by the fungus Magnaporthe oryzae, is the most devastating disease of rice. In our ongoing characterization of the defence mechanisms of rice plants against M. oryzae, a terpene synthase gene OsTPS19 was identified as a candidate defence gene. Here, we report the functional characterization of OsTPS19, which is up-regulated by M. oryzae infection. Overexpression of OsTPS19 in rice plants enhanced resistance against M. oryzae, while OsTPS19 RNAi lines were more susceptible to the pathogen. Metabolic analysis revealed that the production of a monoterpene (S)-limonene was increased and decreased in OsTPS19 overexpression and RNAi lines, respectively, suggesting that OsTPS19 functions as a limonene synthase in planta. This notion was further supported by in vitro enzyme assays with recombinant OsTPS19, in which OsTPS19 had both sesquiterpene activity and monoterpene synthase activity, with limonene as a major product. Furthermore, in a subcellular localization experiment, OsTPS19 was localized in plastids. OsTPS19 has a highly homologous paralog, OsTPS20, which likely resulted from a recent gene duplication event. We found that the variation in OsTPS19 and OsTPS20 enzyme activities was determined by a single amino acid in the active site cavity. The expression of OsTPS20 was not affected by M. oryzae infection. This indicates functional divergence of OsTPS19 and OsTPS20. Lastly, (S)-limonene inhibited the germination of M. oryzae spores in vitro. OsTPS19 was determined to function as an (S)-limonene synthase in rice and plays a role in defence against M. oryzae, at least partly, by inhibiting spore germination.
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http://dx.doi.org/10.1111/pbi.12914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131416PMC
October 2018

Antihypertensive Effects, Molecular Docking Study, and Isothermal Titration Calorimetry Assay of Angiotensin I-Converting Enzyme Inhibitory Peptides from Chlorella vulgaris.

J Agric Food Chem 2018 Feb 1;66(6):1359-1368. Epub 2018 Feb 1.

State Key Laboratory of Bioreactor Engineering, Department of Food Science and Technology, School of Biotechnology, East China University of Science and Technology , Shanghai 200237, People's Republic of China.

The aim of this work is to explore angiotensin I-converting enzyme (ACE) inhibitory peptides from Chlorella vulgaris (C. vulgaris) and discover the inhibitory mechanism of the peptides. After C. vulgaris proteins were gastrointestinal digested in silico, several ACE inhibitory peptides with C-terminal tryptophan were screened. Among them, two novel noncompetitive ACE inhibitors, Thr-Thr-Trp (TTW) and Val-His-Trp (VHW), exhibited the highest inhibitory activity indicated by IC values 0.61 ± 0.12 and 0.91 ± 0.31 μM, respectively. Both the peptides were demonstrated stable against gastrointestinal digestion and ACE hydrolysis. The peptides were administrated to spontaneously hypertensive rats (SHRs) in the dose 5 mg/kg body weight, and VHW could decrease 50 mmHg systolic blood pressure of SHRs (p < 0.05). Molecular docking displayed that both TTW and VHW formed six hydrogen bonds with active site pockets of ACE. Besides, isothermal titration calorimetry assay discovered that VHW could form more stable complex with ACE than TTW. Therefore, VHW was an excellent ACE inhibitor.
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http://dx.doi.org/10.1021/acs.jafc.7b04294DOI Listing
February 2018

Biological and Mechanical Factors Promote the Osteogenesis of Rabbit Artificial Vertebral Laminae: A Comparison Study.

Tissue Eng Part A 2018 07 30;24(13-14):1082-1090. Epub 2018 Jan 30.

Department of Orthopedics, The Fifth People's Hospital of Shanghai, Fudan University , Minhang, China .

Reconstruction of vertebral laminae without epidural scar formation has been challenging. The success of bone formation depends on the biological and mechanical conditions of the surrounding tissues. In this study, we aimed to investigate the roles of biological and mechanical factors in the osteogenesis of artificial laminae. Mesenchymal stem cells derived from rabbit umbilical cord Wharton's jelly were induced for osteogenic differentiation for 3 weeks before seeding on the hydroxyapatite-collagen I scaffolds to construct the tissue-engineered laminae (TEL). TEL were then implanted into the fifth rabbit lumbar vertebrae in both orthotopic lamina (n = 30) and ectopic lamina (n = 30) groups. De novo laminae were examined through histological and radiographic analysis in the 2nd, 4th, 8th, 12th, and 16th weeks postimplantation. Our results showed that de novo laminae formed effectively in both groups, and the osteogenic gene expression levels and cancellous microstructure parameters of de novo laminae in the orthotopic lamina group were significantly higher than those in the ectopic lamina group. In conclusion, the biological stimulation of bone defect initiated the early onset osteogenesis, and the mechanical stimulation of cerebrospinal fluid pulsation stress promoted the osteogenesis of de novo laminae.
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http://dx.doi.org/10.1089/ten.TEA.2017.0426DOI Listing
July 2018

Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice.

Sci Rep 2017 05 30;7(1):2474. Epub 2017 May 30.

Key Laboratory of Pest Monitoring and Green Management, MOA, Department of Plant Pathology, China Agricultural University, Beijing, 100193, China.

Metabolomic and transcriptomic approaches were used to dissect the enhanced disease resistance in the plants harbouring a RNA interfering construct of OsWRKY62 and OsWRKY76 (dsOW62/76) genes. The primary metabolic pathways were activated in dsOW62/76 compared with wild-type (ZH17) plants, revealed by increased accumulation of amino acids and constituents of citric acid cycle etc. Contents of phenolic acids derived from phenylpropanoid pathway were elevated in dsOW62/76 plants. Importantly, phenolamides, conjugates of the phenolic acids with amines, were detected in large number and mostly at higher levels in dsOW62/76 compared with ZH17 plants; however, the free pools of flavonoids were mostly decreased in dsOW62/76. Salicylic acid (SA) and jasmonic acid (JA)/JA-Ile contents were increased in dsOW62/76 and knockout lines of individual OsWRKY62 and OsWRKY76 genes. Transcription of isochorismate synthase (OsICS1) gene was suppressed in dsOW62/76 and in MeJA-treated rice plants, whereas the transcription level of cinnamoyl-CoA hydratase-dehydrogenase (OsCHD) gene for β-oxidation in peroxisome was increased. The calli with OsCHD mutation showed markedly decreased SA accumulation. These results indicate that OsWRKY62 and OsWRKY76 function as negative regulators of biosynthetic defense-related metabolites and provide evidence for an important role of phenylpropanoid pathway in SA production in rice.
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http://dx.doi.org/10.1038/s41598-017-02643-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449406PMC
May 2017

Alternative Splicing of Rice WRKY62 and WRKY76 Transcription Factor Genes in Pathogen Defense.

Plant Physiol 2016 06 18;171(2):1427-42. Epub 2016 Apr 18.

Key Laboratory of Plant Pathology, MOA, China Agricultural University, Beijing 100193, China (Jiq.L., X.C., X.L., X.Z., F.Y., Jia.L., Z.G.); andHoward Hughes Medical Institute, Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (S.Y.H.)

The WRKY family of transcription factors (TFs) functions as transcriptional activators or repressors in various signaling pathways. In this study, we discovered that OsWRKY62 and OsWRKY76, two genes of the WRKY IIa subfamily, undergo constitutive and inducible alternative splicing. The full-length OsWRKY62.1 and OsWRKY76.1 proteins formed homocomplexes and heterocomplexes, and the heterocomplex dominates in the nuclei when analyzed in Nicotiana benthamiana leaves. Transgenic overexpression of OsWRKY62.1 and OsWRKY76.1 in rice (Oryza sativa) enhanced plant susceptibility to the blast fungus Magnaporthe oryzae and the leaf blight bacterium Xanthomonas oryzae pv oryzae, whereas RNA interference and loss-of-function knockout plants exhibited elevated resistance. The dsOW62/76 and knockout lines of OsWRKY62 and OsWRKY76 also showed greatly increased expression of defense-related genes and the accumulation of phytoalexins. The ratio of full-length versus truncated transcripts changed in dsOW62/76 plants as well as in response to pathogen infection. The short alternative OsWRKY62.2 and OsWRKY76.2 isoforms could interact with each other and with full-length proteins. OsWRKY62.2 showed a reduced repressor activity in planta, and two sequence determinants required for the repressor activity were identified in the amino terminus of OsWRKY62.1. The amino termini of OsWRKY62 and OsWRKY76 splice variants also showed reduced binding to the canonical W box motif. These results not only enhance our understanding of the DNA-binding property, the repressor sequence motifs, and the negative feedback regulation of the IIa subfamily of WRKYs but also provide evidence for alternative splicing of WRKY TFs during the plant defense response.
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http://dx.doi.org/10.1104/pp.15.01921DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4902586PMC
June 2016

Ectopic osteogenesis and scaffold biodegradation of nano-hydroxyapatite-chitosan in a rat model.

PLoS One 2015 10;10(8):e0135366. Epub 2015 Aug 10.

The Orthopaedic Department of The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China.

The bone-formation and scaffold-biodegradation processes have not been fully characterized. This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation. The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats. At 2, 4, 6, 8, and 12 weeks post-implantation, the rat femurs were scanned using computerized tomography (CT), and the CT values of the implants were measured and comparatively analyzed. The implants were then harvested and subjected to hematoxylin and eosin (HE) and Masson's trichrome staining, and the percentages of bone area, scaffold area and collagen area were compared between the two groups. The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05). Histological analysis revealed that de novo bone and collagen formation in the pores of the scaffolds gradually increased from 2 weeks post-implantation in both groups and that the scaffold gradually degraded as bone formation proceeded. However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05). In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0135366PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4530870PMC
May 2016

Pseudomonas syringae Effector Avirulence Protein E Localizes to the Host Plasma Membrane and Down-Regulates the Expression of the NONRACE-SPECIFIC DISEASE RESISTANCE1/HARPIN-INDUCED1-LIKE13 Gene Required for Antibacterial Immunity in Arabidopsis.

Plant Physiol 2015 Sep 23;169(1):793-802. Epub 2015 Jul 23.

Department of Energy Plant Research Laboratory (X.-F.X., K.N., X.D., X.C., K.A., F.U., B.R., J.Y., J.C., S.Y.H.), Department of Plant Biology (X.-F.X.), Department of Biochemistry and Molecular Biology (K.W.), and Howard Hughes Medical Institute (S.Y.H.), Michigan State University, East Lansing, Michigan 48824;State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai'an, 271018 Shandong, China (X.D.);Key Laboratory of Plant Pathology, Department of Plant Pathology, China Agricultural University, Beijing 100193, China (X.C.);Genome Institute, Washington University, St. Louis, Missouri 63108 (B.R.); andDepartment of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008 (J.Y.)

Many bacterial pathogens of plants and animals deliver effector proteins into host cells to promote infection. Elucidation of how pathogen effector proteins function not only is critical for understanding bacterial pathogenesis but also provides a useful tool in discovering the functions of host genes. In this study, we characterized the Pseudomonas syringae pv tomato DC3000 effector protein Avirulence Protein E (AvrE), the founding member of a widely distributed, yet functionally enigmatic, bacterial effector family. We show that AvrE is localized in the plasma membrane (PM) and PM-associated vesicle-like structures in the plant cell. AvrE contains two physically interacting domains, and the amino-terminal portion contains a PM-localization signal. Genome-wide microarray analysis indicates that AvrE, as well as the functionally redundant effector Hypersensitive response and pathogenicity-dependent Outer Protein M1, down-regulates the expression of the NONRACE-SPECIFIC DISEASE RESISTANCE1/HARPIN-INDUCED1-LIKE13 (NHL13) gene in Arabidopsis (Arabidopsis thaliana). Mutational analysis shows that NHL13 is required for plant immunity, as the nhl13 mutant plant displayed enhanced disease susceptibility. Our results defined the action site of one of the most important bacterial virulence proteins in plants and the antibacterial immunity function of the NHL13 gene.
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http://dx.doi.org/10.1104/pp.15.00547DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577396PMC
September 2015

Dlf1, a WRKY transcription factor, is involved in the control of flowering time and plant height in rice.

PLoS One 2014 18;9(7):e102529. Epub 2014 Jul 18.

Key Laboratory of Plant Pathology, China Agricultural University, Beijing, China; Zhejiang University, Hangzhou, China.

Flowering time and plant height are important agronomic traits for crop production. In this study, we characterized a semi-dwarf and late flowering (dlf1) mutation of rice that has pleiotropic effects on these traits. The dlf1 mutation was caused by a T-DNA insertion and the cloned Dlf1 gene was found to encode a WRKY transcription factor (OsWRKY11). The dlf1 mutant contains a T-DNA insertion at the promoter region, leading to enhanced accumulation of Dlf1 transcripts, resulting in a semidominant mutation. The dlf1 mutation suppressed the transcription of Ehd2/RID1/OsId1 and its downstream flowering-time genes including Hd1, Ehd1 and Hd3a under both long-day (LD) and short-day (SD) conditions. Knock-down of Dlf1 expression exhibited early flowering at LD condition related to the wild-type plants. Accumulation of Dlf1 mRNA was observed in most tissues, and two splicing forms of Dlf1 cDNAs were obtained (OsWRKY11.1 and OsWRKY11.2). These two proteins showed transactivation activity in yeast cells. Dlf1 protein was found to be localized in the nucleus. Enhanced expression of OsWRKY11.2 or its 5' truncated gene showed similar phenotypes to the dlf1 mutant, suggesting that it might function as a negative regulator. We conclude that Dlf1 acts as a transactivator to downregulate Ehd2/RID1/OsId1 in the signal transduction pathway of flowering and plays an important role in the regulation of plant height in rice.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0102529PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103817PMC
November 2015

Ectopic osteogenesis and scaffold biodegradation of tissue engineering bone composed of chitosan and osteo-induced bone marrow mesenchymal stem cells in vivo.

Chin Med J (Engl) 2014 ;127(2):322-8

Department of Orthopaedic Surgery, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.

Background: Chitosan (CS) scaffolds combined with osteogenically induced bone marrow mesenchymal stem cells (BMSCs) have been proved to be promising substitutes for repairing bone defects. Nevertheless, the bone-forming and scaffold-biodegrading processes are seldom studied. This study aimed to determine the osteogenic ability of CS/osteo-induced BMSC composites by observing the bone-forming process and explore the relationship between bone formation and scaffold biodegradation.

Methods: The CS/osteo-induced BMSC composites (CS+cells group) and the CS scaffolds (CS group) were, respectively, implanted into SD rat thigh muscles. At 2, 4, 6, 8, and 12 weeks postoperatively, the rat femurs were scanned by CT, and the CT values of the implants were measured and comparatively analyzed. Subsequently, the implants were harvested and stained with hematoxylin and eosin and Masson trichrome, and the percentages of bone area, scaffold area, and collagen area were calculated and compared between the two groups.

Results: The imaging results showed that the densities of implants of the two groups gradually increased along with time, but the CT values of implants in the CS+cells group were much higher than in the CS group at the same time point (P < 0.05). The histological results showed that the de novo bone and collagen formed in the pores of the scaffolds and gradually increased since 2 weeks postoperation in both groups, and the scaffold gradually degraded along with the boneforming process. However, the comparative analysis results showed that the CS+cells group gained more de novo bone and collagen formation and had less scaffold than the CS group at the same time point (P < 0.05).

Conclusion: The CS/osteo-induced BMSC composites are excellent bone tissue engineering substitutes, and the scaffold biodegradation is accordant with the bone formation.
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January 2015

[Preliminary effectiveness of I.T.S. locking plate for intra-articular calcaneal fractures].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2013 Sep;27(9):1057-60

Department of Orthopaedics, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai, 200240, P.R.China.

Objective: To summarize the preliminary effectiveness of I.T.S. locking plate for intra-articular calcaneal fractures.

Methods: Between July 2010 and July 2011, 18 cases of intra-articular calcaneal fractures were treated. There were 10 males and 8 females with an average age of 46 years (range, 25-64 years). According to Sanders classification system, there were 3 cases of type II, 9 cases of type III, and 6 cases of type IV. The disease duration was 5-11 days (mean, 6 days). Open reduction and internal fixation with I.T.S. locking plate were performed via an L-shaped lateral extending incision.

Results: Superficial infection occurred in 1 case, and was cured after dressing changing; healing of incision by first intention was obtained in the other cases. Sixteen cases were followed up 14 months on average (range, 12-18 months). X-ray films demonstrated the bone union in all cases with an average union time of 12 weeks (range, 10-14 weeks). No implant failure or irritation of peroneal tendon occurred during follow-up. X-ray films showed subtalar post-traumatic arthritis with mild pain in 1 case at 11 months after operation, which was relieved by conservative treatment. At last follow-up, the Böhler angle was improved from (12.9 +/- 3.2) degrees preoperatively to (33.8 +/- 4.0) degrees postoperatively, showing significant difference (t = 22.78, P = 0.00); the Gissane angle was improved from (83.6 +/- 6.4) degrees preoperatively to (119.9 +/- 8.5) degrees postoperatively, showing significant difference (t = 17.02, P = 0.00). The visual analogue scale (VAS) score was 1.3 +/- 1.2, and the ankle and hindfoot scale of American Orthopaedic Foot and Ankle Society (AOFAS) was 80.3 +/- 7.9 at last follow-up.

Conclusion: Treatment of intra-articular calcaneal fracture with I.T.S. locking plate can obtain a stable fixation, which is a safe and effective method.
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September 2013

Tissue-engineered bone formation in vivo for artificial laminae of the vertebral arch using β-tricalcium phosphate bioceramics seeded with mesenchymal stem cells.

Spine (Phila Pa 1976) 2013 Oct;38(21):E1300-6

From the Department of Orthopedics, Fifth People's Hospital of Shanghai, Fudan University, People's Republic of China.

Study Design: A rabbit laminectomy model was used to evaluate the efficacy of artificial laminae of vertebral arch using bone marrow-derived mesenchymal stem cells (MSCs) transplanted in porous beta-calcium phosphates (β-TCP) bioceramics.

Objective: The aim of this study was to establish artificial lamina of the vertebral arch for bone tissue engineering using β-TCP bioceramics seeded with MSCs in a rabbit model of decompressive laminectomy.

Summary Of Background Data: Decompressive laminectomy may induce various degrees of scar tissue and adhesion formation in the epidural space, and thus is the most common cause of failed back surgery syndrome. However, there is no effective method of bone defect treatment to control and reduce the scar tissue formation.

Methods: MSCs were harvested from New Zealand rabbits (2-week old) by femoral bone marrow extraction. These cells were seeded into porous β-TCP bioceramics and cultivated for up to 3 weeks in the presence of osteogenic supplements. Segmental defects (20 × 8 mm) were created in 48 adult New Zealand rabbits that underwent laminectomy at the L5 to L6 levels. The animals were transplanted with cell media (control), β-TCP bioceramics (group I), or MSC-loaded β-TCP bioceramics (group II). Bone formation was evaluated after operation using scanning electron microscopy, computed tomography, magnetic resonance imaging, histomorphometry, and immunohistochemistry.

Results: Scanning electron microscopy showed that MSCs filled the pores and surfaces of bioceramics in MSC-loaded β-TCP. In addition, significant increases in bone formation were observed in group II compared with other groups. Computed tomography and magnetic resonance imaging at 16 weeks showed that the artificial lamina of the vertebral arch was successfully formed. Hematoxylin-eosin and Masson trichrome staining were used to show the artificial laminae of the vertebral arch and the degraded bioceramics. In addition, immunohistochemistry results showed that the expression of bone morphogenetic protein-2 increased significantly in group II compared with group I at 2,4, and 8 weeks after implantation (P < 0.05).

Conclusion: β-TCP bioceramics seeded with MSCs are a promising source of tissue-engineered bone for the artificial lamina of the vertebral arch.
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http://dx.doi.org/10.1097/BRS.0b013e3182a3cbb3DOI Listing
October 2013

Minimally invasive edge-to-edge mitral repair with or without artificial chordae.

Ann Thorac Surg 2013 Apr 28;95(4):1347-53. Epub 2013 Feb 28.

Department of Cardiothoracic Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Background: This study aims to analyze the midterm outcomes of minimally invasive edge-to-edge mitral valve repair (MVR) with artificial chords (CHORD) or without artificial chords (noCHORD) in patients with mitral regurgitation (MR).

Methods: Records of all patients undergoing edge-to-edge MVR through minithoracotomy at a single institution over a 7-year period were retrospectively reviewed.

Results: A total of 186 patients underwent edge-to-edge MVR through minithoracotomy. Disease etiology was posterior prolapse in 73 (39%) and bileaflet prolapse in 77 (41%). Edge-to-edge sutures were used at A1-P1 in 20 patients (11%), A2-P2 in 136 (73%), and A3-P3 in 30 (16%). Annuloplasty rings were placed in 184 patients (99%), with a mean size of 36±5 mm. Mean follow-up was 2 years (range, 0 to 6), with mean mitral gradient 4±2 mm Hg, MR mild or less in 179 of 186 (96%), 4 (2%) late reoperations, and 1 (0.5%) late death. The CHORD patients (n=71) were more likely than the noCHORD patients (n=115) to have extensive posterior leaflet pathology (p<0.01), had longer clamp and pump times (p<0.01) and were less likely to need leaflet resection (p=0.002), but had similar postoperative courses. At 3 years, freedom from moderate MR was less in CHORD versus noCHORD patients (88±6 versus 100%, p=0.001), but freedom from reoperation was similar (96%±3% versus 99%±1%, p=not significant).

Conclusions: Early results suggest that edge-to-edge MVR can be safe and effective in patients with mitral regurgitation. Edge-to-edge MVR combined with artificial chordae may be useful in selected patients, but with some risk of recurrent moderate MR.
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http://dx.doi.org/10.1016/j.athoracsur.2012.12.026DOI Listing
April 2013

Comparative study on the histomorphology and molecular biology of radial artery conduits in patients with diabetes mellitus who underwent coronary bypass surgery.

Diab Vasc Dis Res 2013 May 12;10(3):208-15. Epub 2012 Sep 12.

Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, People's Republic of China.

We studied the impact of diabetes mellitus (DM) on the radial artery (RA) in 30 patients with DM and 30 non-diabetic patients undergoing coronary artery bypass grafting with autologous RA. RAs were recorded as normal if there was no cellular or stromal tissue between the endothelium and the internal elastic lamina. The RA was normal in 26.7% of diabetic and 76.7% of non-diabetic patients (p = 0.000298). Intimal thickness index and intima:media ratio were higher in the former than in the latter (p < 0.05; p < 0.05), with no significant difference in luminal narrowing (p > 0.05). Electron microscopy scores were lower in the non-diabetic group (p < 0.001); endothelial nitric oxide synthase (eNOS) protein expression and optical density were higher (p < 0.001). Von Willebrand factor and endothelin-1 messenger RNA (mRNA) levels were higher in the DM patients (p < 0.001). The quality of the RA in patients with DM was thus inferior to that in non-diabetic patients. Care should be taken when selecting RA as a conduit in patients with DM.
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http://dx.doi.org/10.1177/1479164112456310DOI Listing
May 2013

Evaluation of Anterior Vertebral Interbody Fusion Using Osteogenic Mesenchymal Stem Cells Transplanted in Collagen Sponge.

Clin Spine Surg 2016 May;29(4):E201-7

Department of Orthopaedics, The Fifth People's Hospital of Fudan University, Shanghai, P.R. China.

Study Design: The study used a rabbit model to achieve anterior vertebral interbody fusion using osteogenic mesenchymal stem cells (OMSCs) transplanted in collagen sponge.

Objective: We investigated the effectiveness of graft material for anterior vertebral interbody fusion using a rabbit model by examining the OMSCs transplanted in collagen sponge.

Summary Of Background Data: Anterior vertebral interbody fusion is commonly performed. Although autogenous bone graft remains the gold-standard fusion material, it requires a separate surgical procedure and is associated with significant short-term and long-term morbidity. Recently, mesenchymal stem cells from bone marrow have been studied in various fields, including posterolateral spinal fusion. Thus, we hypothesized that cultured OMSCs transplanted in porous collagen sponge could be used successfully even in anterior vertebral interbody fusion.

Methods: Forty mature male White Zealand rabbits (weight, 3.5-4.5 kg) were randomly allocated to receive one of the following graft materials: porous collagen sponge plus cultured OMSCs (group I); porous collagen sponge alone (group II); autogenous bone graft (group III); and nothing (group IV). All animals underwent anterior vertebral interbody fusion at the L4/L5 level. The lumbar spine was harvested en bloc, and the new bone formation and spinal fusion was evaluated using radiographic analysis, microcomputed tomography, manual palpation test, and histologic examination at 8 and 12 weeks after surgery.

Results: New bone formation and bony fusion was evident as early as 8 weeks in groups I and III. And there was no statistically significant difference between 8 and 12 weeks. At both time points, by microcomputed tomography and histologic analysis, new bone formation was observed in both groups I and III, fibrous tissue was observed and there was no new bone in both groups II and IV; by manual palpation test, bony fusion was observed in 40% (4/10) of rabbits in group I, 70% (7/10) of rabbits in group III, and 0% (0/10) of rabbits in both groups II and IV.

Conclusions: These findings suggest that mesenchymal stem cells that have been cultured with osteogenic differentiation medium and loaded with collagen sponge could induce bone formation and anterior vertebral interbody fusion. And the rabbit model we developed will be useful in evaluating the effects of graft materials for anterior vertebral interbody fusion. Further study is needed to determine the most appropriate carrier for OMSCs and the feasibility in the clinical setting.
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http://dx.doi.org/10.1097/BSD.0b013e31825ca123DOI Listing
May 2016

The rice ERF transcription factor OsERF922 negatively regulates resistance to Magnaporthe oryzae and salt tolerance.

J Exp Bot 2012 Jun 21;63(10):3899-911. Epub 2012 Mar 21.

Key Laboratory of Plant Pathology, Department of Plant Pathology, China Agricultural University, Beijing, China.

Rice OsERF922, encoding an APETELA2/ethylene response factor (AP2/ERF) type transcription factor, is rapidly and strongly induced by abscisic acid (ABA) and salt treatments, as well as by both virulent and avirulent pathovars of Magnaporthe oryzae, the causal agent of rice blast disease. OsERF922 is localized to the nucleus, binds specifically to the GCC box sequence, and acts as a transcriptional activator in plant cells. Knockdown of OsERF922 by means of RNAi enhanced resistance against M. oryzae. The elevated disease resistance of the RNAi plants was associated with increased expression of PR, PAL, and the other genes encoding phytoalexin biosynthetic enzymes and without M. oryzae infection. In contrast, OsERF922-overexpressing plants showed reduced expression of these defence-related genes and enhanced susceptibility to M. oryzae. In addition, the OsERF922-overexpressing lines exhibited decreased tolerance to salt stress with an increased Na(+)/K(+) ratio in the shoots. The ABA levels were found increased in the overexpressing lines and decreased in the RNAi plants. Expression of the ABA biosynthesis-related genes, 9-cis-epoxycarotenoid dioxygenase (NCED) 3 and 4, was upregulated in the OsERF922-overexpressing plants, and NCED4 was downregulated in the RNAi lines. These results suggest that OsERF922 is integrated into the cross-talk between biotic and abiotic stress-signalling networks perhaps through modulation of the ABA levels.
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http://dx.doi.org/10.1093/jxb/ers079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388842PMC
June 2012

Surgical treatment of a cavernous haemangioma of the heart.

Eur J Cardiothorac Surg 2012 May 21;41(5):1182-3. Epub 2011 Dec 21.

Department of Cardiothoracic Surgery, Nanjing Medical University, Nanjing, China.

Cardiac haemangioma is an extremely rare, benign vascular tumour. A 42-year-old female patient who was previously very active presented with complaints of exertional chest pain as well as lower extremity oedema. Computed tomography and magnetic resonance imaging showed a large mass in the anterior mediastinum originating from the right atrioventricular groove and abutting the superior vena cava, right atrium, right ventricular outflow tract and aortic root. Open biopsy via right mini-thoracotomy revealed cavernous haemangioma. After the biopsy, the patient was treated with three doses of bevacizumab but symptoms worsened and the mass did not regress. Therefore, the patient underwent median sternotomy and resection of the large tumour that encroached upon the right coronary artery. Final histopathological examination revealed cavernous haemangioma. The patient made an uneventful recovery.
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http://dx.doi.org/10.1093/ejcts/ezr153DOI Listing
May 2012

Construction of artificial laminae of the vertebral arch using bone marrow mesenchymal stem cells transplanted in collagen sponge.

Spine (Phila Pa 1976) 2012 Apr;37(8):648-53

Department of Orthopaedics, The Fifth People's Hospital of Fudan University, Shanghai, People's Republic of China.

Study Design: A rabbit laminectomy model was used to evaluate the efficacy of artificial laminae of vertebral arch using bone marrow-derived osteoblasts transplanted in a collagen sponge.

Objective: The objective of this study is to reconstruct the artificial laminae of vertebral arch using bone marrow-derived osteoblasts transplanted in a collagen sponge on a rabbit model.

Summary Of Background Data: Because the laminectomy and semilaminectomy can effectively decompress the spinal cord and expand the vertebral canal, they have been performed as routine surgical procedures. However, long-term follow-up results show that these procedures can lead to many serious complications. A variety of strategies have been used to solve these complications, but there are few experiments to determine the efficacy of reconstructing the laminae of vertebral arch using bone marrow-derived osteoblasts and the collagen sponge.

Methods: The bone marrow mesenchymal stem cells (BMSCs) from the bone marrow in the femur of 2-week-old rabbits were obtained by centrifugation and adhesion. The BMSCs were induced to differentiate into osteoblasts, which were transplanted into collagen sponge to construct the tissue-engineering bone. A total of 48 rabbits were randomly divided into three groups. Lumbar laminectomies were performed on all of the rabbits. Group A was the control. Groups B and C were implanted with collagen sponge and tissue-engineering bone, respectively. The artificial laminae of the vertebral arch were examined qualitatively by imageology and histomorphometry.

Results: The artificial laminae of the vertebral arch successfully formed 4 weeks after the operation in group C; computed tomography examination at 4 weeks showed that the new laminae of vertebral arch were formed, and that the vertebral canal was intact.

Conclusion: The artificial laminae of the vertebral arch can be successfully constructed using tissue engineering of transplanted BMSCs.
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http://dx.doi.org/10.1097/BRS.0b013e31822ecebcDOI Listing
April 2012

Proteomic analysis of myocardial tissue from the border zone during early stage post-infarct remodelling in rats.

Eur J Heart Fail 2011 Mar 10;13(3):254-63. Epub 2010 Dec 10.

Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital Affiliated to Nanjing Medical University, 68 Changle Road, Nanjing, Jiangsu 210006, China.

Aims: Long-term outcome of patients after myocardial infarction (MI) largely depends on the extent of post-infarct remodelling. To explore the molecular mechanism of remodelling, comparative proteomic analysis was undertaken to identify differential myocardial proteome profiles expressed in the border zone of the post-MI heart.

Methods And Results: Two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry were used to identify the differential protein profiles expressed in the border zone at specific time points (Days 0, 1, 4, and 10 post-infarction) in a permanent rat MI model. We identified 96 differential protein spots, corresponding to 69 proteins. Cluster analysis exhibited five main temporal expression patterns corresponding to the three phases of early stage remodelling. The alteration in expression was supported by reverse transcription-polymerase chain reaction, western blotting, and immunohistochemical analysis of three selected proteins. Bioinformatics analysis revealed that the proteins in each pattern were functionally related to specific cell processes in remodelling, such as ischaemia, inflammation, and proliferation.

Conclusion: A differential myocardial proteome profile was identified in the border zone during early stage post-infarct remodelling. Bioinformatics analysis indicated a possible role of these proteins in remodelling. Proteomics data provided the basis for further functional study of these proteins and for identifying potential molecular targets with therapeutic anti-remodelling effects.
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http://dx.doi.org/10.1093/eurjhf/hfq196DOI Listing
March 2011

Antifungal activity of the essential oil of Illicium verum fruit and its main component trans-anethole.

Molecules 2010 Oct 27;15(11):7558-69. Epub 2010 Oct 27.

College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.

In order to identify natural products for plant disease control, the essential oil of star anise (Illicium verum Hook. f.) fruit was investigated for its antifungal activity on plant pathogenic fungi. The fruit essential oil obtained by hydro-distillation was analyzed for its chemical composition by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). trans-Anethole (89.5%), 2-(1-cyclopentenyl)-furan (0.9%) and cis-anethole (0.7%) were found to be the main components among 22 identified compounds, which accounted for 94.6% of the total oil. The antifungal activity of the oil and its main component trans-anethole against plant pathogenic fungi were determined. Both the essential oil and trans-anethole exhibited strong inhibitory effect against all test fungi indicating that most of the observed antifungal properties was due to the presence of trans-anethole in the oil, which could be developed as natural fungicides for plant disease control in fruit and vegetable preservation.
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http://dx.doi.org/10.3390/molecules15117558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259245PMC
October 2010

The stented elephant trunk procedure combined total arch replacement for Debakey I aortic dissection: operative result and follow-up.

Interact Cardiovasc Thorac Surg 2010 Nov 17;11(5):594-8. Epub 2010 Aug 17.

Department of Thoracic and Cardiovascular Surgery, Nanjing Cardiovascular Disease Research Institute, Nanjing First Hospital Affiliated to Nanjing Medical University, 68 Changle Road, Nanjing 210006, China.

The stented elephant trunk technique in aortic arch replacement combined with transaortic stented graft implantation into the descending aorta has been introduced as a means of eliminating the residual false lumen in the descending thoracic aorta and improving long-term outcomes of surgical intervention for Debakey I aortic dissection. This report summarizes the operative and follow-up data with this new procedure. Between August 2004 and May 2009, 28 stented elephant trunk operations were performed for Debakey I aortic dissection at Nanjing First Hospital. A 10 cm long woven Dacron graft was implanted through the aortic arch during hypothermic circulatory arrest. Patent false lumina were evaluated using computed tomography three months after the operation. Mean cardiopulmonary bypass time was 213.2±47.2 min, and selected cerebral perfusion time was 38.8±9.7 min. Hospital mortality was 14.3% (4/28). Thrombus obliteration of the residual false lumen in the descending aorta was observed in 91.7% of the aortic dissections three months postoperatively. The survival rate was 87.5% at five years and the freedom from reoperation rate was 91.7%. Total aortic arch replacement combined with transaortic stented graft implantation into the descending aorta is an effective treatment for Debakey type I aortic dissection.
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http://dx.doi.org/10.1510/icvts.2010.238212DOI Listing
November 2010

Novel surgical method of proximal anastomosis in off-pump coronary artery bypass grafting.

Circ J 2009 Jul 29;73(7):1342-3. Epub 2009 May 29.

Department of Thoracic & Cardiovascular Surgery, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, China.

Background: Cerebral embolization as a result of aortic manipulation has emerged as an important risk factor for the incidence of stroke after off-pump coronary artery bypass grafting (OPACB).

Methods And Results: A new surgical technique for proximal anastomosis without using a side-biting clamp or any proximal anastomotic device in OPACB has been developed and successfully used for proximal anastomosis between a great saphenous vein or radial artery graft and the aorta in OPCAB of 138 patients, with good short-term results.

Conclusions: This novel technique proximal anastomosis in OPACB can be completed in a safe, easy and economical fashion.
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http://dx.doi.org/10.1253/circj.cj-09-0055DOI Listing
July 2009

Radial artery as conduit is safe and effective in coronary bypass surgery in the elderly: single-central results from 326 patients.

Circ J 2009 Jun 14;73(6):1049-54. Epub 2009 Apr 14.

Department of Thoracic & Cardiovascular Surgery, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, China.

Background: As an increasing number of aging patients are being referred for coronary artery bypass grafting (CABG), the radial artery (RA) has gained increasing popularity as a conduit. The aim of this study was to investigate the effectiveness of RA in CABG for the elderly with coronary heart disease.

Methods And Results: Three hundred and twenty-six elderly patients underwent CABG with RA harvesting between January 2000 and June 2008. A total of 377 RA as grafts were collected. The mean number of distal anastomoses per patient was 3.1 while the mean for RA was 1.1. The operative mortality was 3.1%. Twenty patients developed atrial fibrillation, 7 experienced acute renal failure, 9 had neurological accidents, and 4 had myocardial infarction. There was no ischemic complication on hand. Paresthesia in the donor hand was found in 2 patients. A complete follow up was obtained in 77.2% of patients, with a mean time of 53.5 +/-35.9 months. RA patency displayed by coronary angiography was 95.83% after a mean time of 74 months of follow up.

Conclusions: It was proven to be safe and effective to use RA as a secondary arterial graft after the left internal mammary artery in the elderly patients.
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http://dx.doi.org/10.1253/circj.cj-08-1000DOI Listing
June 2009

Tobacco OPBP1 enhances salt tolerance and disease resistance of transgenic rice.

Int J Mol Sci 2008 Dec 11;9(12):2601-13. Epub 2008 Dec 11.

Department of Plant Pathology, China Agricultural University, Beijing, China.

Osmotin promoter binding protein 1 (OPBP1), an AP2/ERF transcription factor of tobacco, has been demonstrated to function in disease resistance and salt tolerance in tobacco. To increase stress tolerant capability of rice, we generated rice plants with an OPBP1 overexpressing construct. Salinity shock treatment with 250 mM NaCl indicated that most of the OPBP1 transgenic plants can survive, whereas the control seedlings cannot. Similar recovery was found by using the seedlings grown in 200 mM NaCl for two weeks. The OPBP1 transgenic and control plants were also studied for oxidative stress tolerance by treatment with paraquat, showing the transgenic lines were damaged less in comparison with the control plants. Further, the OPBP1 overexpression lines exhibited enhanced resistance to infections of Magnaporthe oryzae and Rhizoctonia solani pathogens. Gene expressing analysis showed increase in mRNA accumulation of several stress related genes. These results suggest that expression of OPBP1 gene increase the detoxification capability of rice.
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http://dx.doi.org/10.3390/ijms9122601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635653PMC
December 2008