Publications by authors named "Xueyun Zhang"

16 Publications

  • Page 1 of 1

Baseline serum exosome-derived miRNAs predict HBeAg seroconversion in chronic hepatitis B patients treated with peginterferon.

J Med Virol 2021 Mar 5. Epub 2021 Mar 5.

Department of Liver Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

This study aimed to explore the value of baseline serum exosome-derived miRNAs for predicting HBeAg seroconversion in chronic hepatitis B (CHB) patients treated with peginterferon (Peg-IFN). A total of 120 treatment-naïve HBeAg-positive CHB patients who received Peg-IFN therapy (48 weeks) were enrolled. Next-generation sequencing was performed to screen the serum exosomal miRNAs that were associated with Peg-IFN treatment outcome, and qRT-PCR was used to validate them. The area under the receiver operating characteristic curve (AUROC) was used to evaluate the predictive efficacy of biomarkers. Thirty-three patients (27.5%) achieved HBeAg seroconversion (response group), and 87 patients (72.5%) did not achieve HBeAg seroconversion (nonresponse group). In the identification cohort, 40 serum exosome-derived miRNAs were differentially expressed between the response group (four patients) and the nonresponse group (four patients). In the confirmation cohort, the expression levels of serum exosomal miR-194-5p (p < .001) and miR-22-3p (p < .001) were significantly downregulated in the response group (29 patients) compared to the nonresponse group (83 patients). Multivariate analysis identified baseline serum exosomal miR-194-5p, miR-22-3p, alanine aminotransferase (ALT), and HBV DNA as independent predictors of HBeAg seroconversion (all p < .05). The AUROCs of serum exosomal miRNAs (0.77 and 0.75 for miR-194-5p and miR-22-3p, respectively) were higher than that of ALT (0.70) and HBV DNA (0.69). The combination of exosomal miR-194-5p and miR-22-3p further improved the predictive performance with an AUROC of 0.82. Baseline serum exosomal miR-194-5p and miR-22-3p may serve as novel biomarkers to predict HBeAg seroconversion in CHB patients treated with Peg-IFN.
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http://dx.doi.org/10.1002/jmv.26916DOI Listing
March 2021

A Macromolecular Drug for Cancer Therapy via Extracellular Calcification.

Angew Chem Int Ed Engl 2021 03 17;60(12):6509-6517. Epub 2021 Feb 17.

Cancer Institute, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, China.

Cancer chemotherapy typically relies on drug endocytosis and inhibits tumor cell proliferation via intracellular pathways; however, severe side effects may arise. In this study, we performed a first attempt to develop macromolecular-induced extracellular chemotherapy involving biomineralization by absorbing calcium from the blood through a new type of drug, polysialic acid conjugated with folate (folate-polySia), which selectively induces biogenic mineral formation on tumor cells and results in the pathological calcification of tumors. The macromolecule-initiated extracellular calcification causes cancer cell death mainly by intervening with the glycolysis process in cancer cells. Systemic administration of folate-polySia inhibited cervical and breast tumor growth and dramatically improved survival rates in mice. This study provides an extracellular therapeutic approach for malignant tumor diseases via calcification that is ready for clinical trials and offers new insights into macromolecular anticancer drug discovery.
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http://dx.doi.org/10.1002/anie.202016122DOI Listing
March 2021

Quasi-Vertically-Orientated Antimony Sulfide Inorganic Thin-Film Solar Cells Achieved by Vapor Transport Deposition.

ACS Appl Mater Interfaces 2020 May 6;12(20):22825-22834. Epub 2020 May 6.

School of Photovoltaic and Renewable Energy Engineering, University of New South Wales, Sydney, NSW 2052, Australia.

The one-dimensional photovoltaic absorber material SbS requires crystal orientation engineering to enable efficient carrier transport. In this work, we adopted the vapor transport deposition (VTD) method to fabricate vertically aligned SbS on a CdS buffer layer. Our work shows that such a preferential vertical orientation arises from the sulfur deficit of the CdS surface, which creates a beneficial bonding environment between exposed Cd dangling bonds and S atoms in the SbS molecules. The CdS/VTD-SbS interface recombination is suppressed by such properly aligned ribbons at the interface. Compared to typical [120]-oriented SbS films deposited on CdS by the rapid thermal evaporation (RTE) method, the VTD-SbS thin film is highly [211]- and [121]-oriented and the performance of the solar cell is increased considerably. Without using any hole transportation layer, a conversion efficiency of 4.73% is achieved with device structure of indium tin oxide (ITO)/CdS/SbS/Au. This work provides a potential way to obtain vertically aligned thin films on different buffer layers.
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http://dx.doi.org/10.1021/acsami.0c02697DOI Listing
May 2020

A Stepwise Evaluation of Hepatitis B Virus-Related Acute-on-Chronic Liver Failure to Optimize the Indication for Urgent Liver Transplantation.

Dig Dis Sci 2021 Jan 6;66(1):284-295. Epub 2020 Mar 6.

Department of Infectious Diseases, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai, 200040, China.

Background: Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a dynamic but reversible disease.

Aim: We aimed to clarify whether the change in Chinese Group on the Study of Severe Hepatitis B-ACLF (COSSH-ACLF) grade in HBV-ACLF patients can be used to predict prognosis, and to explore the appropriate conditions for performing urgent liver transplantation.

Methods: We assessed the COSSH-ACLF grades of HBV-ACLF patients at different time points from June 2013 to May 2019 at Huashan Hospital in Shanghai, China, and analyzed the relationship between the change in grade and patient prognosis.

Results: A total of 207 HBV-ACLF patients were enrolled, of which 79 underwent urgent liver transplantation. Their COSSH-ACLF grades were calculated at diagnosis, 3-7 days after diagnosis, and on the final day. Most of the final ACLF grades were consistent with their corresponding grades at days 3-7 after diagnosis (62.5%), while only 44.5% were in accordance with the initial grades at diagnosis. In patients who had a poor prognosis (initial ACLF-3 and ACLF-2 or -3 at days 3-7), the 28-day survival rate was 93.3% in those who underwent transplantation and 6.8% in those who did not (P < 0.0001). However, in patients who had a good prognosis (ACLF-0 or ACLF-1 at days 3-7), the 28-day survival rate was 100% in transplanted patients and 91.5% in non-transplanted patients (P = 0.236).

Conclusions: Reevaluation of the COSSH-ACLF grade 3-7 days after diagnosis could potentially show an indication for urgent liver transplantation.
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http://dx.doi.org/10.1007/s10620-020-06149-xDOI Listing
January 2021

Glucose-6-Phosphate Upregulates Txnip Expression by Interacting With MondoA.

Front Mol Biosci 2019 9;6:147. Epub 2020 Jan 9.

Department of Biochemistry, School of Medicine, Cancer Institute of the Second Affiliated Hospital, Zhejiang University, Hangzhou, China.

The major metabolic fates of glucose in cells are glycolysis and the pentose phosphate pathway, and they share the first step: converting glucose to glucose-6-phosphate (G6P). Here, we show that G6P can be sensed by the transcription factor MondoA/Mlx to modulate Txnip expression. Endogenous knockdown and EMSA (gel migration assay) analyses both confirmed that G6P is the metabolic intermediate that activates the heterocomplex MondoA/Mlx to elicit the expression of Txnip. Additionally, the three-dimensional structure of MondoA is modeled, and the binding mode of G6P to MondoA is also predicted by molecular docking and binding free energy calculation. Finally, free energy decomposition and mutational analyses suggest that certain residues in MondoA, GKL139-141 in particular, mediate its binding with G6P to activate MondoA, which signals the upregulation of the expression of Txnip.
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http://dx.doi.org/10.3389/fmolb.2019.00147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962712PMC
January 2020

Development and Validation of a Novel Model to Predict Liver Histopathology in Patients with Chronic Hepatitis B.

Biomed Res Int 2019 27;2019:1621627. Epub 2019 Feb 27.

Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China.

It is still vague for chronic hepatitis B (CHB) patients with normal or mildly increasing alanine aminotransferase (ALT) level to undergo antiviral treatment or not. The purpose of our study was to establish a noninvasive model based on routine blood test to predict liver histopathology for antiviral therapy. This retrospective study enrolled 258 CHB patients with liver biopsy from the First Hospital of Quanzhou (training cohort, n=126) and Huashan Hospital (validation cohort, n=132). Histologic grading of necroinflammation (G) and liver fibrosis (S) was performed according to the Scheuer scoring system. A novel model, ATPI, including aspartate aminotransferase (AST), total bilirubin (TBil), and platelets (PLT), was developed in training cohort. The area under ROC curves (AUC) of ATPI for predicting antiviral therapy indication was 0.83 in training cohort and was 0.88 in the validation cohort, respectively. Similarly, ATPI also displayed the highest AUC in predicting antiviral therapy indication in CHB patients with normal or mildly increasing ALT level. In conclusion, ATPI is a novel independent model to predict liver histopathology for antiviral therapy in CHB patients with normal and mildly increased ALT levels.
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http://dx.doi.org/10.1155/2019/1621627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415284PMC
July 2019

Rhodium(III)-catalyzed C-H alkynylation of azomethine ylides under mild conditions.

Org Biomol Chem 2014 Dec 17;12(46):9329-32. Epub 2014 Oct 17.

School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Ji'nan 250353, China.

Rh(III)-catalyzed efficient C-H alkynylation of azomethine imines with alkynylated hypervalent iodine is developed under mild conditions. A broad scope of azomethine imines and alkyne substrates is established. The azomethine acts as a masked aldehyde and circumvents its poor directing effect.
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http://dx.doi.org/10.1039/c4ob01596gDOI Listing
December 2014

Rhodium(III)-catalyzed C-C and C-O coupling of quinoline N-oxides with alkynes: combination of C-H activation with O-atom transfer.

Angew Chem Int Ed Engl 2014 Sep 14;53(40):10794-8. Epub 2014 Aug 14.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China); School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan 250353 (China).

[Cp*Rh(III)]-catalyzed C-H activation of arenes assisted by an oxidizing N-O or N-N directing group has allowed the construction of a number of hetercycles. In contrast, a polar N-O bond is well-known to undergo O-atom transfer (OAT) to alkynes. Despite the liability of N-O bonds in both C-H activation and OAT, these two important areas evolved separately. In this report, [Cp*Rh(III)] catalysts integrate both areas in an efficient redox-neutral coupling of quinoline N-oxides with alkynes to afford α-(8-quinolyl)acetophenones. In this process the N-O bond acts as both a directing group for C-H activation and as an O-atom donor.
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http://dx.doi.org/10.1002/anie.201406747DOI Listing
September 2014

Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer.

Virol J 2014 May 12;11:84. Epub 2014 May 12.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan St,, Nangang District, Harbin 150001, China.

Background: Lung cancer is one of the leading causes of deaths from cancer worldwide. Tumor virotherapy using naturally oncolytic Newcastle disease virus (NDV) has been shown to be safe and effective in preclinical studies and clinical trials. Previously, we have reported the NDV D90 strain that was isolated from natural source has an antiproliferative effect in human lung cancer cell line A549.

Methods And Results: In this study, we constructed a reverse genetics system based on the oncolytic NDV D90 strain and generated a recombinant NDV carrying a gene encoding enhanced green fluorescent protein (rNDV-GFP). The rescued virus rNDV-D90 and rNDV-GFP showed the similar characteristics of replication and apoptotic ability in lung cancer A549 cells, which suggested that the recombinant viruses sustained the property of tumor-selective replication and induced apoptosis of tumor cells. The athymic mice bearing implanted lung cancer were treated with the parental D90 virus, the rescued rNDV-D90 and rNDV-GFP via intratumoral injections, respectively. The results showed that the recombinant viruses as well as the parental D90 virus significantly suppressed the loss of body weight and tumor growth.

Conclusions: The study provides a new platform to develop effective therapeutic agents for tumor treatment. The availability of the reverse genetics system for NDV D90 strain will make it possible to develop novel recombinant oncolytic viruses based on the NDV D90 strain for improving the efficacy of tumor treatment.
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http://dx.doi.org/10.1186/1743-422X-11-84DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032357PMC
May 2014

Development of a neutralizing mouse-pig chimeric antibody with therapeutic potential against Haemophilus parasuis in Pichia pastoris.

FEMS Microbiol Lett 2014 May 16;354(2):85-91. Epub 2014 Apr 16.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.

Haemophilus parasuis is one of the most important bacterial diseases of pigs worldwide. The lack of a vaccine against a broad spectrum of strains and the limitation of antimicrobial susceptibility hamper the control of disease. In this study, we cloned the constant regions of gamma heavy chains and kappa light chain of pig lymphocytes in frame with the variable regions of heavy and light chains of mouse monoclonal antibody 1D8, which reacts with all 15 serotypes of H. parasuis and has neutralizing activity. The constructed mouse-pig chimeric antibody was expressed in Pichia pastoris. Results demonstrated that the expressed chimeric antibody inhibited the growth of H. parasuis in vitro. Furthermore, the experiments in mice showed that chimeric antibody increased survival rate of the mice compared with that of the control group (P < 0.05). Importantly, the chimeric antibody partially protected piglets against H. parasuis infection according to the clinical lesion scores and PCR results of H. parasuis in the tissues from piglets of the chimeric antibody-inoculated group and the PBS group. In summary, our results demonstrated that the mouse-pig chimeric antibody could be a therapeutic candidate to prevent the H. parasuis infection and control the prevalence of disease.
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http://dx.doi.org/10.1111/1574-6968.12437DOI Listing
May 2014

Rh(III) -catalyzed hydroacylation reactions between N-sulfonyl 2-aminobenzaldehydes and olefins.

Chemistry 2014 Mar 11;20(12):3283-7. Epub 2014 Mar 11.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (P. R. China); College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074 (P. R. China).

Metal-catalyzed hydroacylation of olefins represents an important atom-economic synthetic process in CH activation. For the first time highly efficient Rh(III) Cp*-catalyzed hydroacylation was realized in the coupling of N-sulfonyl 2-aminobenzaldehydes with both conjugated and aliphatic olefins, leading to the synthesis of various aryl ketones. Occasionally, oxidative coupling occurred when a silver(I) oxidant was used.
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http://dx.doi.org/10.1002/chem.201400022DOI Listing
March 2014

Rhodium(III)-catalyzed redox-neutral C-H arylation via rearomatization.

Org Lett 2014 Mar 3;16(6):1586-9. Epub 2014 Mar 3.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.

Rhodium(III)-catalyzed arylation of arenes bearing a chelating group has been realized via a redox-economy process using 4-hydroxycyclohexa-2,5-dienones as the arylating reagents, leading to the synthesis of 3-arylated phenols. This redox-neutral process proceeds via a C-H activation pathway with rearomatization being the driving force.
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http://dx.doi.org/10.1021/ol500186jDOI Listing
March 2014

Identification of a novel Haemophilus parasuis-specific B cell epitope using monoclonal antibody against the OppA protein.

PLoS One 2014 9;9(1):e84516. Epub 2014 Jan 9.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.

Monoclonal antibody (MAb) 1B3 against Haemophilus parasuis (H. parasuis) was generated by fusing SP2/0 murine myeloma cells and spleen cells from BALB/c mice immunized with the whole-bacterial-cell suspension of H. parasuis HS80 (serotype 5). The MAb 1B3 showed strong reactivity with 15 serotype reference strains of H. parasuis using Dot blot and Western blot analysis. Immunoprecipitation and protein spectral analysis indicated that MAb 1B3 recognized by Oligopeptide permease A (OppA) belongs to the ATP binding cassette transporter family. In addition, a linear B-cell epitope recognized by MAb 1B3 was identified by the screening of a phage-displayed 12-mer random peptide library. Sequence analysis showed that MAb 1B3 was recognized by phages-displaying peptides with the consensus motif KTPSEXR (X means variable amino acids). Its amino acid sequence matched (469)KTPAEAR(475) of H. parasuis OppA protein. A series of progressively truncated peptides were synthesized to define the minimal region that was required for MAb 1B3 binding. The epitope was highly conserved in OppA protein sequences from the isolated H. parasuis strains, which was confirmed by alignment analysis. Furthermore, the minimal linear epitope was highly specific among 75 different bacterial strains as shown in sequence alignments. These results indicated MAb 1B3 might be potentially used to develop serological diagnostic tools for H. parasuis.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0084516PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3887010PMC
September 2014

Anisotropic wetting on checkerboard-patterned surfaces.

Langmuir 2011 Aug 6;27(15):9630-7. Epub 2011 Jul 6.

Department of Chemical and Biomolecular Engineering, Hong Kong University of Science & Technology, Clear Water Bay, Hong Kong.

A series of surfaces with microscale checkerboard patterns consisting of continuous central lines and discontinuous lateral lines were fabricated. The surface wetting properties of these checkerboard patterns were found to be anisotropic. The central continuous lines were found to have a strong influence on the dynamic wetting properties and moving trajectories of the water droplets. The droplets move more easily in the direction parallel to the central continuous lines and less easily in the direction perpendicular to the central continuous lines. Meanwhile, the droplets' moving path tends to incline toward the central continuous lines from a tilting direction. When the microsurface was modified with a layer of nanowire, the surface wettability was found to be isotropic and superhydrophobic.
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http://dx.doi.org/10.1021/la200342wDOI Listing
August 2011

Dynamics of a stick-jump contact line of water drops on a strip surface.

Langmuir 2009 Mar;25(5):3212-8

Department of Chemical and Biomolecular Engineering, Hong Kong University of Science & Technology, Clear Water Bay, Hong Kong.

In this study, we prepared microscale periodic rough structures consisting of parallel strips on a silicon surface. The width of each strip was equal to the gap between the strips, and the silicon surface was silanized with perfluorooctyltrichlorosilane. We studied the wetting characteristics of water drops as they advanced and receded on patterned surfaces in a direction perpendicular to the strip. Water drops were observed to advance or recede in a smooth manner when the strip width was smaller than 32 microm but in a stick-jump manner when the strip width was larger than 50 microm. The regular strip-patterned substrates enabled us to deduce the relationship between the stick-jump behavior and the feature size of the substrate. For surfaces on which water drops showed stick-jump behavior, the oscillation amplitude of the contact angle decreased with decreasing strip width. In addition, the jumping distances of the contact lines, for both advancing and receding water drops, were nearly equal to the strip period. A 2D model was applied to analyze the contact line motion on the patterned surfaces, which showed reasonable agreement with the experimental results.
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http://dx.doi.org/10.1021/la803801yDOI Listing
March 2009

Effect of pattern topology on the self-cleaning properties of textured surfaces.

J Chem Phys 2007 Jul;127(1):014703

Department of Physics, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.

The water contact angle and self-cleaning property of microfabricated surface textures possessing different topologies are compared. In one kind of surface textures, the protruded regions form a connected square network. In the other kind of surface textures, the protruded regions form a regular array of square posts. We find that the water apparent contact angle of the connected textures agrees with the Cassie equation [Discuss. Faraday Soc. 3, 11 (1948)], but that of the disconnected textures is much larger. Nevertheless, the disconnected textures exhibit inferior self-cleaning property, contrary to conventional conception. We discuss the possible reasons for these observations.
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http://dx.doi.org/10.1063/1.2748383DOI Listing
July 2007