Publications by authors named "Xueli Zhao"

43 Publications

Nucleolin-Targeted Ratiometric Fluorescent Carbon Dots with a Remarkably Large Emission Wavelength Shift for Precise Imaging of Cathepsin B in Living Cancer Cells.

Anal Chem 2021 03 15;93(8):4042-4050. Epub 2021 Feb 15.

State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

As one of the most promising biomarkers for numerous malignant tumors, accurate and reliable reporting of Cathepsin B (CTSB) activity is of great significance to achieve efficient diagnosis of cancers at an early stage and predicting metastasis. Here, we report a vigorous ratiometric fluorescent method integrating a cancer-targeting recognition moiety with a remarkably large emission wavelength shift into a single matrix to report CTSB activity sensitively and specifically. As a proof of concept, we synthesized amine-rich carbon quantum dots (CQDs) with a blue fluorescence, which offered an efficient scaffolding to covalently assemble the nucleolin-targeting recognition nucleic acid aptamer AS1411 and a CTSB-cleavable peptide substrate Gly-Arg-Arg-Gly-Lys-Gly-Gly-Cys-COOH that tethered with a near-infrared (NIR) fluorophore chlorin e6 (Ce6-GRRGKGGC, Ce6-Pep), enabling a cancer-targeting and CTSB stimulus-responsive ratiometric nanoprobe AS1411-Ce6-CQDs. Owing to the efficient fluorescence resonance energy transfer (FRET) process from the CQDs to Ce6 inside the assembly of nanoprobe, the blue fluorescence of CQDs at ∼450 nm was remarkably quenched, along with an obvious NIR fluorescence enhancement of Ce6 at ∼650 nm. After selective entry into cancer cells nucleolin-mediated endocytosis, the overexpressed CTSB in lysosome could cleave Ce6-Pep and trigger the Ce6 moiety dissociation from AS1411-Ce6-CQDs, thus leading to the termination of FRET process, achieving the efficient ratiometric fluorescence response toward endogenous CTSB with a remarkably large emission wavelength shift of ∼200 nm from NIR to blue emission region. Notably, the nanoprobe AS1411-Ce6-CQDs exhibited an excellent specificity for ratiometric fluorescent sensing of CTSB activity with an ultralow detection limit of 0.096 ng/mL, demonstrating its promising use for early precise cancer diagnosis in the near future.
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http://dx.doi.org/10.1021/acs.analchem.0c05046DOI Listing
March 2021

Dark-Field Imaging of Cation Exchange Synthesis of CuS@AuS@Au Nanoplates toward the Plasmonic Enhanced Hydrogen Evolution Reaction.

ACS Appl Mater Interfaces 2021 Feb 29;13(5):6515-6521. Epub 2021 Jan 29.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

The development of novel electrocatalysts, especially Pt-free electrocatalysts, is of great significance for evolving hydrogen fuel cells. Two-dimensional materials have many advantages, such as large specific surface area, abundant active edges, and adjustable electronic structure, which provide broad prospects for studying high-performance electrocatalysts. In this paper, CuS@AuS@Au nanoplates (NPs) were synthesized by cation exchange, which showed good catalytic performance toward the hydrogen evolution reaction (HER). Dark-field microscopy can help observe the process of cation exchange in real time to precisely control the synthesis of the composite materials. The synthesized CuS@AuS@Au nanoplates (NPs) exhibited greatly enhanced plasmonic emission, resulting in accelerated chemical conversion and improved HER efficiency. Under 532 nm laser excitation, the overpotential of the HER shifted from 152 to 96 mV at a current density of -10 mA cm. The plasmonic nanocatalysts show exciting prospects in the field of new energy resources.
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http://dx.doi.org/10.1021/acsami.0c20544DOI Listing
February 2021

Predictors of cardiac involvement and survival in patients with primary systemic light-chain amyloidosis: roles of the clinical, chemical, and 3-D speckle tracking echocardiography parameters.

BMC Cardiovasc Disord 2021 Jan 21;21(1):43. Epub 2021 Jan 21.

Xijing Hypertrophic Cardiomyopathy Center, Department of Ultrasound, Xijing Hospital, Xi'an, China.

Background: Light-chain (AL) amyloidosis is the most common type of systemic amyloidosis with poor prognosis. Currently, the predictors of cardiac involvement and prognostic staging systems are primarily based on conventional echocardiography and serological biomarkers. We used three-dimensional speckle tracking echocardiography (STE-3D) measurements of strain, hypothesizing that it could detect cardiac involvement and aid in prediction of mortality.

Methods: We retrospectively analysed 74 consecutive patients with biopsy-proven AL amyloidosis. Among them, 42 showed possible cardiac involvement and 32 without cardiac involvement. LV global longitudinal strain (GLS), global radial strain, global circumferential strain and global area strain (GAS) measurements were obtained.

Results: The GLS and GAS were considered significant predictors of cardiac involvement. The cut-off values discriminating cardiac involvement were 16.10% for GLS, 32.95% for GAS. During the median follow-up of 12.5 months (interquartile range 4-25 months), 20 (27%) patients died. For the Cox proportional model survival analysis, heart rate, cardiac troponin T, NT-proBNP levels, E/e', GLS, and GAS were univariate predictors of death. Multivariate Cox model showed that GLS ≤ 14.78% and cardiac troponin T ≥ 0.049 mg/l levels were independent predictors of survival.

Conclusions: STE-3D measurements of LV myocardial mechanics could detect cardiac involvement in patients with AL amyloidosis; GLS and cardiac biomarkers can provided prognostic information for mortality prediction.
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http://dx.doi.org/10.1186/s12872-021-01856-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819214PMC
January 2021

Direct anterior approach versus posterolateral approach in total hip arthroplasty: a meta-analysis of results on early post-operative period.

J Orthop Surg Res 2021 Jan 19;16(1):69. Epub 2021 Jan 19.

Department of Medical Oncology, Weifang People's Hospital, no. 151 Guangwen Road, Weifang, 260041, China.

Background: This study was performed to compare the advantage and disadvantage of posterolateral approach (PLA) and direct anterior approach (DAA) in total hip arthroplasty (THA).

Methods: Relevant trials were identified via a search of the Cochrane Central Register of Controlled Trials and PubMed from inception to 1 June 2019. A meta-analysis was performed to compare postoperative perioperative and radiographic outcomes between DAA and PLA in THA with respect to the hospital stay, blood loss, incision length, operative time, complications, and femoral and cup component position. The Harris Hip Score (HHS) was also assessed before and after 6 months postoperatively.

Results: Nine eligible studies involving 22698 adult patients (DAA group, n = 2947; PLA group, n = 19751) were identified for analysis. Compared with the PLA group, the DAA group had shorter hospital stay and achieved better HHS within 6 months after operation (P < 0.05), but the HHS was no significant differences between the two groups over 6 months (P > 0.05). The DAA group had significantly longer operative time, more blood loss, and complications than the PLA group (P < 0.05). In addition, the femoral component positioned in neutral and cup component inclination angle was comparable between both groups (P > 0.05); however, cup component anteversion angle was significantly larger in the PLA group (P < 0.05).

Conclusion: Patients in the DAA group had higher HHS within 6 months and shorter hospital stay. The DAA could offer rapid early functional recovery after THA compared with the PLA. However, the DAA group often required longer operative time and had more blood loss. Furthermore, there was a higher early complication rate. Therefore, we believe that the direct anterior approach was a more difficult technique. The surgeon should be a well-trained joint surgeon with extensive prior hip replacement experience before performing THA through a DAA, and DAA was not suitable for beginners performing THA. In addition, we did not observe the difference with regard to the femoral component position and cup component inclination angle except for the smaller cup component anteversion angle in DAA group.
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http://dx.doi.org/10.1186/s13018-021-02218-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816494PMC
January 2021

Feeding Fungal-Pretreated Corn Straw Improves Health and Meat Quality of Lambs Infected with Gastrointestinal Nematodes.

Animals (Basel) 2020 Sep 16;10(9). Epub 2020 Sep 16.

Jilin Provincial Key Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.

Infections with gastrointestinal nematodes (GIN) adversely affect meat color in lambs. Although white-rot fungi (WRF) pretreatment increases nutritional value and fiber digestion of corn straw for lambs, whether it can improve meat quality of lambs infected with GINs is unknown. The objective of this experiment was to study effects of feeding WRF-pretreated corn straw on the health and meat quality of lambs infected with GINs. Sixteen healthy Ujumqin lambs were orally drenched with 3rd-stage GINs larvae and randomly divided into two dietary treatments of control (CON) and WRF diets for 70 days of feeding. Results showed that feeding WRF-pretreated corn straw decreased * and * values ( < 0.05) and increased * value ( < 0.01) of both longissimus thoracis et lumborum (LTL) and semimembranosus (SM) muscles of lambs infected with GINs. Feeding WRF-pretreatment corn straw decreased fecal egg count ( 0.014) and increased packed cell volume ( 0.013) of lambs from 28 d of feeding and increased plasma iron content ( 0.008) of lambs from 56 d of the feeding. Feeding WRF-pretreatment corn straw decreased myosin heavy-chain (MyHC)-I ( 0.032) and MyHC-IIα ( 0.025) content in LTL muscle and MyHC-I ( 0.022) and MyHC-IIβ ( 0.048) in SM muscle of lambs. In conclusion, although there were no significant changes in the content of most amino acids or increased intensity of better flavor compounds, meat quality and health of lambs infected with GINs was significantly improved by feeding WRF-pretreated corn straw due to increased PCV and meat color and tenderness.
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http://dx.doi.org/10.3390/ani10091659DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552176PMC
September 2020

RINT1 is a new suppression target to reduce colon cancer cell growth, migration and invasion through regulating ZW10/NAG-1 expression.

Mol Cell Biochem 2020 Aug 4. Epub 2020 Aug 4.

Department of Pathology, Tangshan Gongren Hospital, Lubei District, No. 27 Wenhua Road, Tangshan, 063000, Hebei, China.

This study explored the function and mechanism of RAD50 interactor 1 (RINT1), Zeste white 10 (ZW10) or nonsteroidal anti-inflammatory drug-activated gene-1 (NAG-1) in cell growth of colon cancer. The mRNA expression of RINT1 was suppressed in patients with colon cancer. In addition, disease free survival (DFS) in colon cancer patients with high expression of RINT1, ZW10 and NAG-1 were promoted compared with colon cancer patients with low expression of RINT1, ZW10 and NAG-1. Over-expression of RINT1, ZW10 or NAG-1 reduced colon cancer cell growth, migration and invasion in vitro model. In contrast, down-regulation of RINT1, ZW10 or NAG-1 promoted colon cancer cell growth, migration and invasion in vitro model. Over-expression of RINT1 promoted while down-regulation of RINT1 suppressed ZW10 and NAG-1 expression. Following knockdown of ZW10 and NAG-1 protein expressions, RINT1 induced changes of colon cancer cell growth, migration and invasion were inhibited. Taken together, it is indicated that RINT1 is a new suppression target to reduce cell growth, migration and invasion of colon cancer by targeting ZW10/NAG-1.
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http://dx.doi.org/10.1007/s11010-020-03858-9DOI Listing
August 2020

Tuning the properties of atomically precise gold nanoclusters for biolabeling and drug delivery.

Chem Commun (Camb) 2020 Aug;56(62):8766-8769

Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou 450001, China.

A structure-determined gold-levonorgestrel nanocluster, Au10(C21H27O2)10 (Au10NC), has been obtained via solvent induced crystal transformation from Au8(C21H27O2)8 (Au8NC), accompanied by a fluorescence color change from green to yellow. A cationic polymer, poly(allylamine hydrochloride) (PAH), was chosen to assemble with Au10NC to prepare monodisperse and stable particles (Au10NC-PAH) with an increased quantum yield of 49.8%. The as-fabricated Au10NC-PAH realized effective antibody-mediated actin imaging and sustained drug release.
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http://dx.doi.org/10.1039/d0cc03498cDOI Listing
August 2020

High-potency white-rot fungal strains and duration of fermentation to optimize corn straw as ruminant feed.

Bioresour Technol 2020 Sep 8;312:123512. Epub 2020 May 8.

Jilin Provincial Key Laboratory of Grassland Farming, Northeast Institute of Geography and Agoecology, Chinese Academy of Sciences, Changchun, Jilin 130102, China. Electronic address:

Five white-rot fungi Pleurotus ostreatus, Lentinus edodes, Hericium erinaceus, Pleurotus eryngii and Flammulina filiformis were studied (solid-state incubation and in vitro gas production) to determine lignin degradation and optimal duration of fermentation of corn straw. All fungi significantly decreased lignin, with optimal reductions after 28 d. Although cellulose also decreased, L. edodes and P. eryngii minimized these losses. In intro dry matter digestibility, total volatile fatty acid concentration and total gas production of fermented corn straw decreased (P < 0.001) as fermentation was prolonged, with improved rumen fermentability for all fungal treatments except F. filiformis. Total gas production in L. edodes did not decrease but peaked on day 28, whereas F. filiformis reduced methane emission. In conclusion, fermentation of corn straw with P. eryngii or L. edodes for 28 d degraded lignin and improved nutritional value as ruminant feed.
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http://dx.doi.org/10.1016/j.biortech.2020.123512DOI Listing
September 2020

Reduction of depression-like behavior in rat model induced by ShRNA targeting norepinephrine transporter in locus coeruleus.

Transl Psychiatry 2020 05 4;10(1):130. Epub 2020 May 4.

CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China.

Depression may be associated with reduced monoamine neurotransmission, particularly serotonin and norepinephrine (NE). Reuptake of NE by the norepinephrine transporter (NET) is the primary mechanism by which many of the antidepressants are high-affinity substrates for NET. This study aimed to examine the effect of lentivirus-mediated shRNA targeting NET in locus coeruleus (LC) on depression-like behaviors of rats. We randomly assigned 60 male Wistar rats to 6 experimental groups: (1) Control group: without chronic unpredictable mild stress (CUMS) and without NET-shRNA treatment; (2) shRNA group: without CUMS + NET-shRNA; (3) CUMS group: 3-week CUMS without NET-shRNA; (4) CUMS + nonsense shRNA group; (5) CUMS + amygdala (Amy)-shRNA group; (6) CUMS+ locus coeruleus (LC)-shRNA group. First, recombinant lentiviral vector expressing shRNA (ShRNA-629, ShRNA-330, ShRNA-1222, ShRNA-1146 or ShRNA- negative control) against NET were produced, and their efficiency in knocking down of NET in PC12 cells were assessed by Q-PCR and western blot analysis. Second, shRNA was injected into the rat LC bilaterally to investigate whether it could prevent the depressive-like behavior induced by 3-week CUMS. Third, we tested the depressive-like behavior of the rats in the forced swimming test, the open field test, the sucrose preference test, as well as the body weight gain at the end of the seventh week. Finally, the protein expressions of NET was measured by western blot and the NE levels were measured by high performance liquid chromatography. Q-PCR and western blot showed that the ShRNA-1146 had the best interference efficiency targeting on NET in PC12 cells (p < 0.01). Compared to the depression model group, the immobility time in the forced swimming test was significantly reduced (p < 0.01), but the sucrose preference and the total scores in the open field test were significantly increased (all p < 0.01) in the group treated with shRNA in LC. Furthermore, compared with the depression model group, NET levels were significantly decreased (p < 0.01), but NE levels were significantly increased in the group treated with shRNA in LC (p < 0.05). Our findings suggest that Lentivirus-mediated shRNA targeting NET in LC downregulated NET both in vitro and in vivo, resulting in a significant decrease in depressive-like behavior of rats.
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http://dx.doi.org/10.1038/s41398-020-0808-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198598PMC
May 2020

The Design of a Frame-Like ZnO FBAR Sensor for Achieving Uniform Mass Sensitivity Distributions.

Sensors (Basel) 2020 Apr 23;20(8). Epub 2020 Apr 23.

Piezoelectric Device Laboratory, School of Mechanical Engineering and Mechanics, Ningbo University, Ningbo 315211, China.

In this paper, an infinite circular ZnO thin film bulk acoustic resonator (FBAR) with a frame-like electrode operating at the thickness-extensional (TE) mode is studied. Two-dimensional scalar differential equations established for the problem in the Cartesian coordinate system are successfully solved by transforming them into normal Bessel equations and modified Bessel equations in the cylindrical coordinate system. Resonant frequencies and vibration distributions are obtained for this frame-like FBAR sensor. A nearly uniform mass sensitivity distribution in the active area is achieved by designing proper electrode size and mass ratio of the driving electrode to the ZnO film. Numerical results show that compared with the reported ring electrode FBAR sensor, the novel frame-like electrode FBAR can achieve a maximum optimization ratio (up to 97.90%) on the uniformity of the mass sensitivity distribution in the active area under the same structural parameters, which is also higher than the optimization ratio 77.63% obtained by the reported double-ring electrode design. Moreover, the mechanism to achieve a very uniform mass sensitivity distribution in the active area by the frame-like electrode is explained in detail according to dispersion curves. Namely, when the resonant frequency of the FBAR sensor is close to the cut-off frequency of the active region in the dispersion curve, the mass sensitivity distribution is nearly uniform. These conclusions provide a theoretical guidance for the design and optimization of ZnO FBAR mass sensors with high performance.
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http://dx.doi.org/10.3390/s20082408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219592PMC
April 2020

Stereospecific interactions between chiral inorganic nanomaterials and biological systems.

Chem Soc Rev 2020 Apr 16;49(8):2481-2503. Epub 2020 Mar 16.

College of Chemistry, Zhengzhou University, Zhengzhou 450001, China.

Chirality is ubiquitous in nature and plays mysterious and essential roles in maintaining key biological and physiological processes. As biological systems display high selectivity for chiral biomolecules, chiral bio-nanoscience has become a popular research field during the last decade. Homochirality, as an essential attribute of natural compounds (l-amino acids, d-sugars, etc.), inspired the emergence of synthetic chiral nanomaterials, which in turn impacted their biological functions and fates. This review is a comprehensive overview of the interactions between chiral inorganic nanostructures and biosystems. We start with the recent progress in biocompatible chiral nanomaterials and focus on stereospecific biological interactions ranging from enantioselective reactions in applications such as sensing and catalysis to chirality-dependent controllable manipulation of cell behaviours and finally to enantiopure nanoplatforms for improved disease therapy. We also discuss the current challenges and future potential of these chiral nanotechnologies in biomedicine and bioengineering, provide strategies to overcome these barriers and offer a future perspective.
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http://dx.doi.org/10.1039/d0cs00093kDOI Listing
April 2020

A Peptide-Functionalized Magnetic Nanoplatform-Loaded Melatonin for Targeted Amelioration of Fibrosis in Pressure Overload-Induced Cardiac Hypertrophy.

Int J Nanomedicine 2020 27;15:1321-1333. Epub 2020 Feb 27.

Department of Ultrasound of Xijing Hospital, Xijing Hypertrophic Cardiomyopathy Center, Fourth Military Medical University, Xi'an 710032, People's Republic of China.

Introduction: Currently, the unsatisfactory treatment of cardiac hypertrophy is due to the unbridled myocardial fibrosis. Melatonin has been demonstrated to ameliorate cardiac hypertrophy and its accompanied fibrosis in previous studies. But it is not clinically appealing due to its short-lasting time against the hostile microenvironment when administered orally.

Methods: Herein, to address this, poly (lactide) polycarboxybetaine (PLGA-COOH) accompanied by cardiac homing peptide (CHP) and superparamagnetic iron oxide nanoparticles (SPIONs) were used to establish a novel drug delivery and transportation strategy for melatonin via a facile two-step emulsion method. This study characterized these nanoparticles (CHP-mel@SPIONs) and tested their delivery to the hypertrophied heart and their effect on myocardial hypertrophy and fibrosis in an animal model of pressure overload-induced cardiac hypertrophy.

Results: The engineered magnetic nanoparticles of CHP-mel@SPIONs were spherical (diameter = 221 ± 13 nm) and had a negative zeta potential of -19.18 ± 3.27 mV. The CHP-mel@SPIONs displayed excellent drug encapsulation capacities of SPIONs (75.27 ± 3.1%) and melatonin (77.69 ± 6.04%) separately, and their magnetic properties were characterized by constructing magnetic hysteresis curves and exhibited no remnant magnetization or coercivity. The animal experiments showed that compared with mel@SPIONs, CHP-mel@SPIONs accumulated more in the heart, especially in the presence of an external magnetic field, with in vivo echocardiography and RT-PCR and histological assessments confirming the amelioration of the myocardial hypertrophy and fibrosis with low drug doses.

Conclusion: This simple biocompatible dual-targeting nanoagent may be a potential candidate for the guided clinical therapy of heart disease.
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http://dx.doi.org/10.2147/IJN.S235518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7051809PMC
June 2020

Quantitative Analysis of Left Ventricular Flow Dynamics in Latent Obstructive Hypertrophic Cardiomyopathy Using Vector Flow Mapping.

Cardiology 2020 25;145(4):227-235. Epub 2020 Feb 25.

Ultrasound Department of Xijing Hospital, Xijing Hypertrophic Cardiomyopathy Center, Fourth Military Medical University, Xi'an, China,

Objectives: This study aimed to assess left ventricular (LV) energy loss (EL), circulation and vortex area using vector flow mapping (VFM) in patients with latent obstructive hyper-trophic cardiomyopathy (LOHCM) and nonobstructive hypertrophic cardiomyopathy (NOHCM).

Methods: Fourteen LOHCM patients, 10 NOHCM patients, and 11 healthy individuals were evaluated by transthoracic echocardiography. An offline VFM workstation was used to analyze the LV blood flow patterns and fluid dynamics. The hemodynamic parameters, EL, circulation, and vortex area in 7 cardiac phases were calculated and analyzed.

Results: Compared with controls and NOHCM patients, EL was significantly higher in -LOHCM patients during the rapid ejection phase, slow ejection (SE) phase, and isovolumetric relaxation phase (p < 0.05). LOHCM patients also showed increased circulation during SE compared to the other two groups (p < 0.05). The ability to discriminate between NOHCM and LOHCM was assessed by the area under the receiver-operating characteristic curve (AUC), and EL during SE was found to have the largest AUC (0.964); the best cutoff value was 6.34 J/m3/s, with a sensitivity of 100% and specificity of 80%.

Conclusions: The VFM technique can detect abnormal changes of LV EL and vortex characteristics in hypertrophic cardiomyopathy patients. Compared with controls and NOHCM patients, the LOHCM patients have worse systolic and diastolic functions.
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http://dx.doi.org/10.1159/000504665DOI Listing
February 2020

Melatonin Inhibits Formation of Mitochondrial Permeability Transition Pores and Improves Oxidative Phosphorylation of Frozen-Thawed Ram Sperm.

Front Endocrinol (Lausanne) 2019 8;10:896. Epub 2020 Jan 8.

Jilin Provincial Key Laboratory of Grassland Farming, Northeast Institute of Geography and Agoecology, Chinese Academy of Sciences, Changchun, China.

Structural and functional damages to mitochondria of frozen-thawed sperm are a typical cryoinjury, with mitochondrial permeability transition pore (MPTP) formation being the hallmark change. Mitochondria are both a primary synthesis site and principle target for melatonin; this compound can directly inhibit MPTP formation and therefore confer protection at a mitochondrial level. The objective was to determine effects of melatonin on MPTP opening, viability, motility, and oxidative phosphorylation (OXPHOS) of frozen-thawed ram sperm. Ram semen was diluted in glucose-egg yolk buffer with 0 or 10 M melatonin (frozen and frozen + melatonin groups, respectively) and slow frozen, with fresh semen as Control. In frozen-thawed sperm, melatonin inhibited MPTP opening and lactate concentrations and improved sperm viability, motility, acetyl-CoA concentration and adenosine triphosphate (ATP) production. With regard to the underlying physiological mechanism, melatonin suppressed movement of citrate synthase, isocitrate dehydrogenase, oxoglutarate dehydrogenase complex, and F0F1-ATP synthase permeability from mitochondrial to cytosolic fractions induced by MPTP opening; furthermore, it increased mRNA expressions of respiratory chain complex components and activities of complexes I, II, III, and IV and thereby improved oxygen consumption capacity in frozen-thawed sperm. In conclusion, melatonin improved OXPHOS of frozen-thawed ram sperm, attributed to inhibition of cryopreservation-induced MPTP opening.
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http://dx.doi.org/10.3389/fendo.2019.00896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960123PMC
January 2020

Periostin Promotes Colorectal Tumorigenesis through Integrin-FAK-Src Pathway-Mediated YAP/TAZ Activation.

Cell Rep 2020 01;30(3):793-806.e6

State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102, China; Cancer Research Center of Xiamen University, Xiamen 361102, China. Electronic address:

Periostin is a multifunctional extracellular matrix protein involved in various inflammatory diseases and tumor metastasis; however, evidence regarding whether and how periostin actively contributes to inflammation-associated tumorigenesis remains elusive. Here, we demonstrate that periostin deficiency significantly inhibits the occurrence of colorectal cancer in azoxymethane/dextran sulfate sodium-treated mice and in Apc mice. Moreover, periostin deficiency attenuates the severity of colitis and reduces the proliferation of tumor cells. Mechanistically, stromal fibroblast-derived periostin activates FAK-Src kinases through integrin-mediated outside-in signaling, which results in the activation of YAP/TAZ and, subsequently, IL-6 expression in tumor cells. Conversely, IL-6 induces periostin expression in fibroblasts by activating STAT3, which ultimately facilitates colorectal tumor development. These findings provide the evidence that periostin promotes colorectal tumorigenesis, and identify periostin- and IL-6-mediated tumor-stroma interaction as a promising target for treating colitis-associated colorectal cancer.
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http://dx.doi.org/10.1016/j.celrep.2019.12.075DOI Listing
January 2020

Quantifying global CH and NO footprints.

J Environ Manage 2019 Dec 17;251:109566. Epub 2019 Sep 17.

School of Management, China University of Mining & Technology (Beijing), Beijing, 100083, PR China; State Key Laboratory of Coal Resources and Safe Mining, University of Mining & Technology (Beijing), Beijing, 100083, PR China; Harvard China Project, School of Engineering and Applied Sciences, Harvard University, MA, 02138, United States. Electronic address:

This study aims to quantify global CH and NO footprints in 2012 in terms of 181 economies and 20 world regions based on the official national emission accounts from the UNFCCC database and the global multi-region input-output accounts from the EORA database. Global total CH and NO emissions increased by 15.0% in 2012 compared to 1990, mainly driven by increasing demands of agricultural and energy products. Mainland China, Western Europe, the USA, Southeast Asia and Sub-Saharan Africa were identified as the largest five CH footprint contributors (55.6% of the global total), while Mainland China, the USA, Western Europe, Brazil and Sub-Saharan Africa as the largest NO footprint contributors (59.2% of the global total). In most developed economies, the CH and NO footprints were much higher than their emissions on the production side, but opposite picture is observed in emerging economies. 36.4% and 24.8% of the global CH and NO emissions in 2012 were associated with international trade, respectively. Substantial energy-related CH and agricultural CH and NO emissions were transferred from developed countries to developing countries. Several nations within Kyoto targets have reduced their CH and NO emissions significantly between 1990 and 2012, but the generally-believed success is challenged when viewing from the consumption side. Mainland China, Southeast Asia, Sub-Saharan Africa, Brazil, Middle East and India witnessed prominent increase of CH and NO footprints in the same period. The structure and spatial patterns of global CH and NO footprints shed light on the role of consumption-side actions and international cooperation for future non-CO GHG emission reduction.
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http://dx.doi.org/10.1016/j.jenvman.2019.109566DOI Listing
December 2019

AIE Triggers the Circularly Polarized Luminescence of Atomically Precise Enantiomeric Copper(I) Alkynyl Clusters.

Angew Chem Int Ed Engl 2020 06 18;59(25):10052-10058. Epub 2019 Sep 18.

College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, China.

Atomically precise enantiomeric metal clusters are scarce, and copper(I) alkynyl clusters with intense circularly polarized luminescence (CPL) responses have not been reported. A pair of chiral alkynyl ligands, (R/S)-2-diphenyl-2-hydroxylmethylpyrrolidine-1-propyne (abbreviated as R/S-DPM) we successfully prepared and single crystals were characterized of optically pure enantiomeric pair of atomically-precise copper(I) clusters, [Cu (R/S-DPM) ](PF ) (denoted as R/S-Cu ), which feature bright red luminescence and CPL with a high luminescence anisotropy factor (g ). A dilute solution containing R/S-Cu was nonluminescent and CPL inactive at room temperature. Crystallization- and aggregation-induced emission (CIE and AIE, respectively) contribute to the triggering of the CPL of R/S-Cu in the crystalline and aggregated states. Their AIE behavior and good biocompatibility indicated applications of these copper(I) clusters in cell imaging in HeLa and NG108-15 cells.
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http://dx.doi.org/10.1002/anie.201908909DOI Listing
June 2020

Atomically Precise Gold-Levonorgestrel Nanocluster as a Radiosensitizer for Enhanced Cancer Therapy.

ACS Nano 2019 07 28;13(7):8320-8328. Epub 2019 Jun 28.

College of Chemistry and Molecular Engineering , Zhengzhou University , Zhengzhou 450001 , China.

Gold nanoclusters have become promising radiosensitizers due to their ultrasmall size and robust ability to adsorb, scatter, and re-emit radiation. However, most of the previously reported gold nanocluster radiosensitizers do not have a precise atomic structure, causing difficulties in understanding the structure-activity relationship. In this study, a structurally defined gold-levonorgestrel nanocluster consisting of Au(CHO) (AuNC) with bright luminescence (58.7% quantum yield) and satisfactory biocompatibility was demonstrated as a nanoradiosensitizer. When the AuNCs were irradiated with X-rays, they produced reactive oxygen species (ROS), resulting in irreversible cell apoptosis. As indicated by tumor formation experiments, tumorigenicity was significantly suppressed after one radiotherapy treatment with the AuNCs. In addition, compared with tumors treated with X-rays (4 Gy) alone, tumors treated with the nanosensitizer exhibited an inhibition rate of 74.2%. This study contributes to the development of atomically precise gold nanoclusters as efficient radiosensitizers.
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http://dx.doi.org/10.1021/acsnano.9b03767DOI Listing
July 2019

Effects of Feeding Garlic Powder on Growth Performance, Rumen Fermentation, and the Health Status of Lambs Infected by Gastrointestinal Nematodes.

Animals (Basel) 2019 Mar 20;9(3). Epub 2019 Mar 20.

Jilin Provincial Key Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.

For the study, forty lambs were weighed and assigned into two treatments to determine the effects of feeding garlic powder on growth performance, rumen fermentation, and the health status of lambs infected with gastrointestinal nematodes (GINs). The lambs were fed with a basal diet without or with 50 g/kg garlic powder for 84 d. Data were analyzed by a general linear or mixed model of SAS software and differences were considered statistically significant if ≤ 0.05. Results showed that garlic powder supplementation increased the lambs' average daily gain ( = 0.025), digestibility of dry matter ( = 0.019), and crude protein ( = 0.007). No significant changes were observed on the dry matter intake, feed conversion ratio, as well as the apparent digestibility of lipid and fiber. An interactive effect between treatment and feeding day was observed on ruminal pH ( < 0.001) and ammonia nitrogen concentration ( < 0.001). Ruminal pH ( < 0.001) and ammonia nitrogen concentration ( < 0.001) decreased with garlic powder supplementation, while ammonia nitrogen concentration increased ( = 0.001) with the extension of the feeding period. Garlic powder supplementation increased the total volatile fatty acid concentration ( < 0.001) in the rumen fluid, the molar proportion of acetic acid ( = 0.002), propionic acid ( < 0.001), and isovaleric acid ( = 0.049), but it decreased the ratio of acetic acid to propionic acid ( = 0.015). The lambs' fecal egg count decreased ( < 0.001), but the packed cell volume and body condition scores of lambs increased ( < 0.001) with garlic powder supplementation. In conclusion, feeding garlic powder increased growth performance, feed digestion, rumen fermentation, and the health status of lambs infected with GINs.
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http://dx.doi.org/10.3390/ani9030102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466378PMC
March 2019

Bone Marrow Mesenchymal Stromal Cell-Derived Periostin Promotes B-ALL Progression by Modulating CCL2 in Leukemia Cells.

Cell Rep 2019 02;26(6):1533-1543.e4

State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China; Cancer Research Center of Xiamen University, Xiamen, Fujian 361102, China. Electronic address:

Periostin (POSTN) is a multifunctional extracellular component that regulates cell-matrix interactions and cell-cell crosstalk. POSTN deletion significantly decreases leukemia burden in mice; however, the underlying mechanisms by which POSTN promotes B cell acute lymphoblastic leukemia (B-ALL) progression remain largely unknown. Here, we demonstrate that bone marrow (BM)-derived mesenchymal stromal cells (BM-MSCs) express higher levels of POSTN when co-cultured with B-ALL cells in vitro and in vivo. POSTN deficiency in BM-MSCs significantly decreases CCL2 expression in co-cultured B-ALL cells in vitro and in vivo. Moreover, POSTN treatment increases expression of CCL2 in B-ALL cells by activating the integrin-ILK-NF-κB pathway. Conversely, CCL2 treatment upregulates expression of POSTN in BM-MSCs via STAT3 activation. Furthermore, there is a positive correlation between POSTN expression and CCL2 level in the BM of mice and patients with B-ALL. These findings suggest that B-ALL cell-derived CCL2 contributes to the increased leukemia burden promoted by BM-MSC-derived POSTN.
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http://dx.doi.org/10.1016/j.celrep.2019.01.034DOI Listing
February 2019

Manganese(ii) phosphate nanosheet assembly with native out-of-plane Mn centres for electrocatalytic water oxidation.

Chem Sci 2019 Jan 2;10(1):191-197. Epub 2018 Oct 2.

Key Laboratory of Applied Surface and Colloid Chemistry , Ministry of Education , School of Chemistry and Chemical Engineering , Shaanxi Normal University , Xi'an 710119 , China . Email: ; Email:

Nature selects Mn-clusters as catalysts for water oxidation, which is a significant reaction in photosynthesis. Thus, it is of critical importance to develop Mn-based superstructures and study their catalytic details for water-splitting-based renewable energy research. Herein, we report a manganese(ii) phosphate nanosheet assembly with asymmetric out-of-plane Mn centers from the transformation of amine-intercalated nanoplates for efficient electrocatalytic water oxidation in neutral aqueous solutions. From structural and computational studies, it is found that the native out-of-plane Mn centers with terminal water ligands are accessible and preferential oxidation sites to form active intermediates for water oxidation. In addition, the asymmetry can stabilize the key Mn intermediate, as demonstrated by electrochemical and spectrometric studies. This study delivers a convenient strategy to prepare unique nanosheet assemblies for electrocatalysis and fundamental understandings of oxygen evolution chemistry.
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http://dx.doi.org/10.1039/c8sc03764gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333235PMC
January 2019

Intracellular delivery of nucleic acid by cell-permeable hPP10 peptide.

J Cell Physiol 2019 07 4;234(7):11670-11678. Epub 2018 Dec 4.

Hubei Key Lab of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, China.

Although gene therapy offers hope against incurable diseases, nonreplicating transduction vectors remain lacking. We have previously characterized a cell-penetrating peptide hPP10 for the delivery of various cargoes; however, whether hPP10 can mediate nucleic acid delivery is still unknown. Here, examining via different ways, we demonstrate that hPP10 stably complexes with plasmid DNA (pDNA) and safely mediates nucleic acid transfection. hPP10 can mediate GFP-, dsRed-, and luciferase-expressing plasmids into cells with nearly the same efficiency as commercial transfection reagents Turbofectin or Lipofect. Furthermore, hPP10 can mediate Cre fusion protein delivery and pDNA transfection simultaneously in the Cre/loxp system in vitro. In addition, hPP10 fused with an RNA-binding domain can mediate delivery of small interfering RNA into cells to silence the reporter gene expression. Collectively, our results suggest that hPP10 is an option for nucleic acid delivery with efficiencies similar to that of commercial reagents.
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http://dx.doi.org/10.1002/jcp.27826DOI Listing
July 2019

Carbon Nanotubes with Cobalt Corroles for Hydrogen and Oxygen Evolution in pH 0-14 Solutions.

Angew Chem Int Ed Engl 2018 Nov 18;57(46):15070-15075. Epub 2018 Oct 18.

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710119, China.

Water splitting is promising to realize a hydrogen-based society. The practical use of molecular water-splitting catalysts relies on their integration onto electrode materials. We describe herein the immobilization of cobalt corroles on carbon nanotubes (CNTs) by four strategies and compare the performance of the resulting hybrids for H and O evolution. Co corroles can be covalently attached to CNTs with short conjugated linkers (the hybrid is denoted as H1) or with long alkane chains (H2), or can be grafted to CNTs via strong π-π interactions (H3) or via simple adsorption (H4). An activity trend H1≫H3>H2≈H4 is obtained for H and O evolution, showing the critical role of electron transfer ability on electrocatalysis. Notably, H1 is the first Janus catalyst for both H and O evolution reactions in pH 0-14 aqueous solutions. Therefore, this work is significant to show potential uses of electrode materials with well-designed molecular catalysts in electrocatalysis.
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http://dx.doi.org/10.1002/anie.201807996DOI Listing
November 2018

Outsourcing natural resource requirements within China.

J Environ Manage 2018 Dec 17;228:292-302. Epub 2018 Sep 17.

School of Economics and Management, University of Chinese Academy of Sciences, Beijing, 100190, PR China. Electronic address:

Consumption demands are final drivers for the extraction and allocation of natural resources. This paper investigates demand-driven natural resource requirements and spatial outsourcing within China in 2012 by using the latest multi-regional input-output model. Exergy is adopted as a common metric for natural resources input. The total domestic resource exergy requirements amounted to 125.5 EJ, of which the eastern area contributed the largest share of 44.5%, followed by the western area (23.9%), the central area (23.0%) and the northeastern area (8.6%). Investment was the leading final demand category, accounting for 52.9% (66.4 EJ) of national total embodied resource use (ERU). The total trade volumes of embodied resource were equivalent to 69.6% of the total direct resource input (DRI), mostly transferred from the central and western regions such as Inner Mongolia, Shanxi, Shaanxi and Xinjiang to the eastern regions such as Jiangsu, Zhejiang, Guangdong and Shanghai. The northeastern and eastern areas had physical net imports of 1213.5 PJ and 38452.6 PJ, while the central and western inland areas had physical net exports of 6364.5 PJ and 33301.5 PJ, respectively. Shanghai, Beijing, Zhejiang, Jiangsu and Guangdong had prominent ERUs which respectively were 101.6, 12.6, 11.7, 8.4 and 4.3 times of their DRIs. The ERUs of Inner Mongolia, Shaanxi, Shanxi, Ningxia and Guizhou were equal to only 17.6%, 25.3%, 27.9%, 46.0% and 50.2% of their DRIs, respectively. Regional uneven development resulted in imbalanced resource requirements across China. The findings can provide a deep understanding of China's resource-driven economic development mode, and contribute to reducing regional resource footprints and their environment outcomes under the "new normal economy".
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http://dx.doi.org/10.1016/j.jenvman.2018.08.107DOI Listing
December 2018

Efficient penetration of Scp01-b and its DNA transfer abilities into cells.

J Cell Physiol 2019 05 19;234(5):6539-6547. Epub 2018 Sep 19.

Department of Pathology and Immunology, Medical School, China Three Gorges University, Yichang, China.

The in vivo application potential of viral-based gene delivery approaches is hindered by a risk of insertional oncogenesis. Of the many delivery methods, cell-penetrating peptides (CPP)-based delivery has good biocompatibility and biodegradability. However, low efficiency is still the disadvantage of CPPs-based nucleic acid transfection, and delivery efficiency may vary from different CPPs. Here, we describe Scp01-b, as a new CPP, which can enter cultured cell lines and primary cultured cells examined by fluorescence microscopy and quantitative assay, the internalization process is a concentration, temperature, and incubation time-dependent manner. Scp01-b does not insert into the membrane directly and its uptake is mediated through endocytosis pathway. Moreover, Scp01-b could mediate the uptake of plasmid DNA into the Caski and HSC-T6 cells, and we noted that Scp01-b-mediated transfection efficiency was nearly the same with traditional liposome (TurboFectin)-mediated transfection. These findings suggest that Scp01-b can act as a useful tool for non-viral-based delivery in further application such as reprogramming and gene editing.
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http://dx.doi.org/10.1002/jcp.27392DOI Listing
May 2019

Sphaerisporangium rhizosphaerae sp. nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell. Arg).

Int J Syst Evol Microbiol 2018 Sep 16;68(9):2860-2865. Epub 2018 Jul 16.

1​Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.

A novel actinomycete, designated strain NEAU-mq3, was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell. Arg) collected from Xianglu Mountain in Heilongjiang Province, north-east China, and characterized by using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives 'Sphaerisporangium dianthi' NEAU-CY18 (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291 (98.8 %). Morphological and chemotaxonomic properties of strain NEAU-mq3 were also consistent with the description of the genus Sphaerisporangium. The whole-cell sugars were madurose, mannose, ribose and glucose. The menaquinones were MK-9(H2), MK-9(H4), MK-9(H0) and MK-9(H6). The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol mannoside, an unidentified polar lipid and an unidentified phospholipid. The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c. DNA-DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-mq3 and its most closely related strains, which further clarified their relatedness and demonstrated that NEAU-mq3 could be distinguished from these strains. Therefore, it is concluded that strain NEAU-mq3 represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rhizosphaerae sp. nov. is proposed. The type strain is NEAU-mq3 (=CGMCC 4.7429=JCM 32389).
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http://dx.doi.org/10.1099/ijsem.0.002912DOI Listing
September 2018

Cardiomyocyte-targeted and 17β-estradiol-loaded acoustic nanoprobes as a theranostic platform for cardiac hypertrophy.

J Nanobiotechnology 2018 Mar 30;16(1):36. Epub 2018 Mar 30.

Department of Ultrasound, Xijing Hypertrophic Cardiomyopathy Center, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.

Background: Theranostic perfluorocarbon nanoprobes have recently attracted attention due to their fascinating versatility in integrating diagnostics and therapeutics into a single system. Furthermore, although 17β-estradiol (E2) is a potential anti-hypertrophic drug, it has severe non-specific adverse effects in various organs. Therefore, we have developed cardiomyocyte-targeted theranostic nanoprobes to achieve concurrent targeted imaging and treatment of cardiac hypertrophy.

Results: We had successfully synthesized E2-loaded, primary cardiomyocyte (PCM) specific peptide-conjugated nanoprobes with perfluorocarbon (PFP) as a core (PCM-E2/PFPs) and demonstrated their stability and homogeneity. In vitro and in vivo studies confirmed that when exposed to low-intensity focused ultrasound (LIFU), these versatile PCM-E2/PFPs can be used as an amplifiable imaging contrast agent. Furthermore, the significantly accelerated release of E2 enhanced the therapeutic efficacy of the drug and prevented systemic side effects. PCM-E2/PFPs + LIFU treatment also significantly increased cardiac targeting and circulation time. Further therapeutic evaluations showed that PCM-E2/PFPs + LIFU suppressed cardiac hypertrophy to a greater extent compared to other treatments, revealing high efficiency in cardiac-targeted delivery and effective cardioprotection.

Conclusion: Our novel theranostic nanoplatform could serve as a potential theranostic vector for cardiac diseases.
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http://dx.doi.org/10.1186/s12951-018-0360-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877324PMC
March 2018

Lentzea soli sp. nov., an actinomycete isolated from soil.

Int J Syst Evol Microbiol 2018 May 14;68(5):1496-1501. Epub 2018 Mar 14.

Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.

A novel actinobacterium, designated strain NEAU-LZC 7, was isolated from soil collected from Mount Song and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LZC 7 belonged to the genus Lentzea, with highest sequence similarity to Lentzea violacea JCM 10975 (98.1 %). Morphological and chemotaxonomic characteristics of the strain also supported its assignment to the genus Lentzea. However, DNA-DNA relatedness, physiological and biochemical data showed that strain NEAU-LZC 7 could be distinguished from its closest relative. Therefore, strain NEAU-LZC 7 represents a novel species of the genus Lentzea, for which the name Lentzea soli sp. nov. is proposed, with NEAU-LZC 7 (=CCTCC AA 2017027=JCM 32384) as the type strain.
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http://dx.doi.org/10.1099/ijsem.0.002698DOI Listing
May 2018

Streptomyces flavalbus sp. nov., an actinobacterium isolated from rhizosphere of maize (Zea mays L.).

Antonie Van Leeuwenhoek 2018 Jul 11;111(7):1047-1054. Epub 2018 Jan 11.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.

A novel actinobacterium, designated strain NEAU-QY24, was isolated from the rhizosphere of corn (Zea mays L.). A polyphasic approach was employed to determine the taxonomic status of strain NEAU-QY24. The isolate was found to have chemical and morphological properties of the genus Streptomyces, with high 16S rRNA gene sequence similarity to Streptomyces lanatus JCM 4332 (98.3%) and clustered phylogenetically with Streptomyces lannensis JCM 16578 (98.2%). The cell wall was found to contain meso-diaminopimelic acid and the whole cell sugars were identified as glucose and ribose. The predominant menaquinones were identified as MK-9(H), MK-9(H) and MK-9(H). The phospholipid profile was found to consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were identified as iso-C, C, anteiso-C and C. A combination of DNA-DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-QY24 and its closely related strains, which clarified their relatedness and demonstrated that strain NEAU-QY24 could be distinguished from these strains. These data indicate that the isolate should be recognised as a new species of the genus Streptomyces, for which the name Streptomyces flavalbus sp. nov. is proposed. The type strain is NEAU-QY24 (= CGMCC 4.7400 = DSM 104539).
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http://dx.doi.org/10.1007/s10482-017-1004-6DOI Listing
July 2018

Ultrasound-mediated nanobubble destruction (UMND) facilitates the delivery of A10-3.2 aptamer targeted and siRNA-loaded cationic nanobubbles for therapy of prostate cancer.

Drug Deliv 2018 Nov;25(1):226-240

a Department of Ultrasound , Chinese PLA General Hospital , Beijing , China.

The Forkhead box M1 (FoxM1) transcription factor is an important anti-tumor target. A novel targeted ultrasound (US)-sensitive nanobubble that is likely to make use of the physical energy of US exposure for the improvement of delivery efficacy to target tumors and specifically silence FoxM1 expression appears as among the most potential nanocarriers in respect of drug delivery. In this study, we synthesized a promising anti-tumor targeted FoxM1 siRNA-loaded cationic nanobubbles (CNBs) conjugated with an A10-3.2 aptamer (siFoxM1-Apt-CNBs), which demonstrate high specificity when binding to prostate-specific membrane antigen (PSMA) positive LNCaP cells. Uniform nanoscaled siFoxM1-Apt-CNBs were developed using a thin-film hydration sonication, carbodiimide chemistry approaches, and electrostatic adsorption methods. Fluorescence imaging as well as flow cytometry evidenced the fact that the siFoxM1-Apt-CNBs were productively developed and that they specifically bound to PSMA-positive LNCaP cells. siFoxM1-Apt-CNBs combined with ultrasound-mediated nanobubble destruction (UMND) significantly improved transfection efficiency, cell apoptosis, and cell cycle arrest in vitro while reducing FoxM1 expression. In vivo xenografts tumors in nude-mouse model results showed that siFoxM1-Apt-CNBs combined with UMND led to significant inhibition of tumor growth and prolonged the survival of the mice, with low toxicity, an obvious reduction in FoxM1 expression, and a higher apoptosis index. Our study suggests that siFoxM1-Apt-CNBs combined with UMND might be a promising targeted gene delivery strategy for therapy of prostate cancer.
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http://dx.doi.org/10.1080/10717544.2017.1422300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6058493PMC
November 2018