Publications by authors named "Xueli Liu"

88 Publications

Structure of the mannose phosphotransferase system (man-PTS) complexed with microcin E492, a pore-forming bacteriocin.

Cell Discov 2021 Apr 6;7(1):20. Epub 2021 Apr 6.

State Key Laboratory of Membrane Biology, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.

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http://dx.doi.org/10.1038/s41421-021-00253-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021565PMC
April 2021

The Mechanisms and Functions of GDF-5 in Intervertebral Disc Degeneration.

Orthop Surg 2021 Apr 4. Epub 2021 Apr 4.

Spinal Surgery Department, Hospital (T.C.M) Affiliated to Southwest Medical University, Luzhou, China.

Intervertebral disc degeneration (IDD) is widely recognized as the main cause of low back pain, which leads to disability in aging populations and induces great losses both socially and economically worldwide. Unfortunately, current treatments for IDD are aimed at relieving symptoms instead of preserving disc structure and function. Researchers are forged to find new promising biological therapeutics to stop, and even reverse, IVD degeneration. Recently, the injection of growth factors has been shown to be a promising biological therapy for IDD. A number of growth factors have been investigated to modulate the synthesis of the extracellular matrix (ECM) through a variety of pathogenetic biological mechanisms, including suppressing inflammatory process and down-regulating degrading enzymes. However, growth factors, including Transforming Growth Factor-β (TGF-β), Fibroblast Growth Factor (FGF), and Insulin-like Growth Factor-1 (IGF-1), may induce unwanted blood vessel in-growth, which accelerates the process of IDD. On the contrary, studies have demonstrated that injection of GDF-5 into the intervertebral disc of mice can effectively alleviate the degeneration of the intervertebral disc, which elicits their response via BMPRII and will not induce blood vessel in-growth. This finding suggests that GDF-5 is more suitable for use in IDD treatment compared with the three other growth factors. Substantial evidence has suggested that GDF-5 may maintain the structure and function of the intervertebral disc (IVD). GDF-5 plays an important role in IDD and is a very promising therapeutic agent for IDD. This review is focused on the mechanisms and functions of GDF-5 in IDD.
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http://dx.doi.org/10.1111/os.12942DOI Listing
April 2021

Narrative therapy to relieve stigma in oral cancer patients: A randomized controlled trial.

Int J Nurs Pract 2021 Apr 5:e12926. Epub 2021 Apr 5.

Department of Nursing, Qilu Hospital, Shandong University, Jinan, China.

Aims And Objectives: This study aimed to evaluate the efficacy of narrative therapy in relieving stigma in oral cancer patients who underwent major surgical treatment.

Background: Health-related stigma compromises mental health and life quality in people with physical or mental abnormalities. Narrative therapy has been implemented to overcome stigma among populations in a diversity of disease states. However, the effectiveness of narrative therapy in relieving stigma among patients with oral cancer is not known.

Design: This study was a randomized controlled trial, in which 100 oral cancer patients were selected and randomly assigned to the 'narrative therapy' group, who received narrative therapy treatment in addition to standard care, and the 'control' group, who was provided standard care only.

Methods: This research combined measurement of several questionnaires to evaluate stigma. Analysis of variance and paired t tests were employed for data analysis.

Results: Findings in this study demonstrated that narrative therapy treatment effectively relieved oral cancer patients' sense of shame, reducing overall stigma and significantly improving self-esteem and social relationships.

Conclusions: Narrative therapy was demonstrated to be a promising therapeutic intervention for stigma relief in oral cancer patients.

Summary Statement: What is already known about this topic? Stigma is common among oral cancer patients, especially those who undergo major surgical resection. Psychological intervention is highly recommended in addition to postsurgical care. Narrative therapy might help relieve stigma among oral cancer patients. What this paper adds? Conventional postsurgical care did not improve oral cancer patients' psychological situation. A 7-week intervention of narrative therapy treatment significantly reduced stigma and enhanced sense of self-worth and social support among oral cancer patients. The implications of this paper: Narrative therapy can be clinically accepted as a therapeutic approach to assist oral cancer patients with stigma after major surgery. Tailored approaches, including patient-customized methods, should be encouraged in conducting narrative therapy.
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http://dx.doi.org/10.1111/ijn.12926DOI Listing
April 2021

Porcine hemagglutinating encephalomyelitis virus induces atypical autophagy via opposite regulation of expression and nuclear translocation of transcription factor EB.

Vet Microbiol 2021 Apr 17;255:109015. Epub 2021 Feb 17.

Key Laboratory ofZoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address:

Porcine hemagglutinating encephalomyelitis virus (PHEV) displays neurotropism and induces atypical autophagy. However, the exact mechanisms mediating autophagy induced by PHEV remains uncharacterized. Transcription factor EB (TFEB) is a master transcriptional regulator playing a key role in autophagy and its activity is regulated by MTORC1 kinase on the surface of lysosomes. We first found that PHEV infection decreases TFEB expression, while it activates TFEB by inhibiting MTORC1 activation, indicating that TFEB plays a complex role in the process of PHEV-induced autophagy through opposite regulation of its expression and activity. Furthermore, this study preliminarily demonstrated that PHEV replication is dependent on TFEB expression.
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http://dx.doi.org/10.1016/j.vetmic.2021.109015DOI Listing
April 2021

Performance and substance transformation of low-pH and neutral-pH biofilters treating complex gases containing hydrogen sulfide, ammonia, acetic acid, and toluene.

Environ Sci Pollut Res Int 2021 Feb 13. Epub 2021 Feb 13.

Beijing Research Center of Sustainable Urban Drainage System and Risk Control, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.

Two biofilters with low pH and neutral pH were operated on pilot scale for the treatment of complex gases containing hydrogen sulfide, ammonia, acetic acid, and toluene during 205 days. Under the coexistence of complex gases, the low-pH biofilter (LPB) had higher removal efficiency (RE) for hydrogen sulfide and toluene, and the maximum efficiencies were 99.24% and 99.90% respectively, while the neutral-pH biofilter (NPB) had higher REs of ammonia and acetic acid, up to 99.90% and 99.92% respectively. Higher pressure drop up to 622 Pa was achieved in the LPB, most likely caused by the special structure of fungi different from bacteria. Determination of the concentration of carbon-based intermediates revealed the dominant microbial removal of acetic acid and clarified the relationship between the generation of intermediate and the performance of biofilters. Respective amount of CO in the inlet and outlet showed that the mineralization capacity of the NPB was higher than that of the LPB, and it was more influenced by empty bed residence time (EBRT). The proportion of different forms of nitrogen and sulfur in the filler indicated that the removal of ammonia in the LPB mainly depended on the adsorption by moisture, while that in the NPB was microbial degradation, which was also the main removal pathway of sulfur regardless of pH condition. The removal and transformation of different substances in coexisting complex gases showed different characteristics in the LPB and NPB respectively.
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http://dx.doi.org/10.1007/s11356-021-12602-9DOI Listing
February 2021

Determination and pharmacokinetic analysis of ticarcillin disodium-clavulanate potassium for injection in rat plasma by UPLC-ESI-MS/MS.

J Int Med Res 2020 Dec;48(12):300060520967822

Department of Pharmaceutical of Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, China.

Objective: To establish a specific and rapid ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) method for measuring ticarcillin and clavulanate levels in rat plasma.

Methods: A Waters ACQUITY BEH C18 column (50 mm × 2.1 mm, 1.7 μm) and SCIEX QTRAP® LC-MS/MS System were used. Analyses were conducted to optimize the chromatographic and MS conditions, and the pharmacokinetic parameters of ticarcillin and clavulanate were assessed.

Results: Linear relationships were observed in the ranges of 10 to 10,000 ng/mL for ticarcillin R (r = 0.9967) 30 to 10,000 ng/mL for ticarcillin S (r = 0.9961), and 30 to 10,000 ng/mL for clavulanate (r = 0.9981). The average extraction recoveries of all compounds ranged from 86.9% to 96.4%. The pharmacokinetic parameters of the ticarcillin R and S isomers in rats were distinctive. The ticarcillin R and S isomers and clavulanate were rapidly absorbed . Ticarcillin S and clavulanate had similar elimination rates, whereas that of ticarcillin R was slower.

Conclusion: A UPLC-ESI-MS/MS method was developed and validated for the determination of ticarcillin and clavulanate in rat plasma.
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http://dx.doi.org/10.1177/0300060520967822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770263PMC
December 2020

Study on the regulation of earthworm physiological function under cadmium stress based on a compound mathematical model.

Environ Toxicol Pharmacol 2020 Nov 18;80:103499. Epub 2020 Sep 18.

College of Resources and Environmental Science, Northeast Agricultural University, Harbin, China. Electronic address:

A cadmium (Cd) stress test was carried out on Eisenia fetida in artificial soil. Six Cd concentration gradient solutions (0, 50, 100, 125, 250 and 500 mg/kg) were prepared. Two treatment groups, short-term stress and long-term stress, were established. The former lasted for 10 days, and the latter lasted for 30 days. The Biolog ECO-microplate culture method was used to determine the utilization of the 31 carbon sources by the microbes in earthworm homogenate. The total protein content (TP), peroxidase activity (POD), catalase activity (CAT), superoxide dismutase activity (SOD), glutathione peroxidase activity (GPX), glutathione-S-transferase activity (GST), malondialdehyde content (MDA) and acetylcholinesterase activity (AChE) in earthworm were determined in order to investigate the regulation of oxidative stress and the functional diversity of microbial communities in earthworms under Cd stress. By combining the entropy weight method (EW) and the technique for order preference by similarity to an ideal solution model (TOPSIS), the physiological functional indices of earthworms were assessed objectively and scientifically, and the physiological changes under the different stress periods were evaluated. The results showed that a Cd-tolerant dominant population appeared in the microbial community under Cd stress. In the short-term test, oxidative stress were more effective in coping with Cd stress than the microbial community, and oxidative stress regulated the microbial community functional diversity. Under long-term Cd stress, the regulatory effect was weak or non-existent. In this study, a new evaluation model was established to explore the regulation process of earthworm on its oxidation stress and the functional diversity of microbial communities under Cd stress, and provide a theoretical basis for revealing the detoxification mechanism of earthworms.
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http://dx.doi.org/10.1016/j.etap.2020.103499DOI Listing
November 2020

Styrene removal with an acidic biofilter with four packing materials: Performance and fungal bioaerosol emissions.

Environ Res 2020 12 30;191:110154. Epub 2020 Aug 30.

Beijing Engineering Research Center of Sustainable Urban Sewage System Construction and Risk Control, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China; Key Laboratory of Urban Stormwater System and Water Environment, Ministry of Education, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.

The packing material used in acidic biofilters (ABFs) has a significant impact on styrene removal. The bioaerosol emission was rarely considered when evaluating the packing materials in the ABFs. Four ABFs packed with ceramsite, compost, lava and polyurethane (PU) were developed and compared for their styrene removal and fungal bioaerosol emissions characteristics over 529 days. The removal efficiencies of styrene in the ABFs were higher under the condition of longer empty bed residence time (EBRT) and lower inlet concentration. The maximum styrene elimination capacities of the ABFs with ceramsite, compost, lava and PU were 74.57, 87.81, 67.13 and 101.88 g/m h, respectively. A lower pressure drop and the highest fungi count were observed in the ABF packed with PU. The emissions concentrations of fungal bioaerosols at the humidity of 63.5% were lower than those at a humidity of 42.7% and it increased with the air velocity. Additionally, the concentrations of fungal bioaerosols emitted from the ABFs packed with PU were 2168 ± 145-3661 ± 257 CFU/m, which was 33%-90% lower than those of the other three ABFs. The particle size distributions of the fungal bioaerosols emitted from the ABFs packed with PU and compost were mainly centered around large particles. Considering the removal of styrene and the fungal bioaerosols emissions, PU was the optimal packing material for ABF.
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http://dx.doi.org/10.1016/j.envres.2020.110154DOI Listing
December 2020

Field-Induced Wettability Gradients for No-Loss Transport of Oil Droplets on Slippery Surfaces.

ACS Appl Mater Interfaces 2020 Aug 12;12(34):38723-38729. Epub 2020 Aug 12.

National Center for International Research on Green Optoelectronics, Guangdong Provincial Key Laboratory of Optical Information Materials and Technology & Institute of Electronic Paper Displays, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, P. R. China.

Transporting oil droplets is crucial for a wide range of industrial and biomedical applications but remains highly challenging due to the large contact angle hysteresis on most solid surfaces. A liquid-infused slippery surface has a low hysteresis contact angle and is a highly promising platform if sufficient wettability gradient can be created. Current strategies used to create wettability gradient typically rely on the engineering of the chemical composition or geometrical structure. However, these strategies are inefficient on a slippery surface because the infused liquid tends to conceal the gradient in the chemical composition and small-scale geometrical structure. Magnifying the structure, on the other hand, will significantly distort the surface topography, which is unwanted in practice. In this study, we address this challenge by introducing a field-induced wettability gradient on a flat slippery surface. By printing radial electrodes array, we can pattern the electric field, which induces gradient contact angles. Theoretical analysis and experimental results reveal that the droplet transport behavior can be captured by a nondimensional electric Bond number. Our surface enables no-loss transport of various types of droplets, which we expect to find important applications such as heat transfer, anticontamination, microfluidics, and biochemical analysis.
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http://dx.doi.org/10.1021/acsami.0c06389DOI Listing
August 2020

Simultaneous removal of bioaerosols, odors and volatile organic compounds from a wastewater treatment plant by a full-scale integrated reactor.

Process Saf Environ Prot 2020 Dec 4;144:2-14. Epub 2020 Jul 4.

Beijing Engineering Research Center of Sustainable Urban Sewage System Construction and Risk Control, Beijing University of Civil Engineering and Architecture, Beijing 100044, China.

Biological control of odors and bioaerosols in wastewater treatment plants (WWTPs) have gained more attention in recent years. The simultaneous removal of odors, volatile organic compounds (VOCs) and bioaerosols in each unit of a full-scale integrated-reactor (FIR) in a sludge dewatering room was investigated. The average removal efficiencies (REs) of odors, VOCs and bioaerosols were recorded as 98.5 %, 94.7 % and 86.4 %, respectively, at an inlet flow rate of 5760 m/h. The RE of each unit decreased, and the activated carbon adsorption zone (AZ) played a more important role as the inlet flow rate increased. The REs of hydrophilic compounds were higher than those of hydrophobic compounds. For bioaerosols, roughly 35 % of airborne heterotrophic bacteria (HB) was removed in the low-pH zone (LPZ) while over 30 % of total fungi (TF) was removed in the neutral-pH zone (NPZ). Most bioaerosols removed by the biofilter (BF) had a particle size larger than 4.7 μm while bioaerosols with small particle size were apt to be adsorbed by AZ. The microbial community in the BF changed significantly at different units. Health risks were found to be associated with HS rather than with bioaerosols at the FIR outlet.
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http://dx.doi.org/10.1016/j.psep.2020.07.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341965PMC
December 2020

Gas Phase Dehydration of Glycerol to Acrolein Over Nickel Phosphate Catalysts.

J Nanosci Nanotechnol 2020 Dec;20(12):7680-7685

School of Materials and Chemical Engineering, Chuzhou University, Chuzhou 239000, China.

We investigated the catalytic performance of glycerol conversion to acrolein on nickel phosphates samples (NiP-T ( = 300,400,500,600, and 700 °C)). The textural property, acidity of the fresh catalyst and carbon content of the used NiP-500 were also determined. The results showed that NiP was amorphous under the appropriate calcination temperature. The textural property, acid amount and strength were important in this reaction. Glycerol conversion was proportional to the acid amount of the sample. After 2 h on stream, NiP-500 with the largest pore size, largest acid amount and largest number of moderate acid sites had the maximum catalytic performance (89% glycerol conversion and 64% acrolein selectivity). NiP-700 showed the lowest performance (48% glycerol conversion and 34% acrolein selectivity), which is due to the lowest surface area, pore size and the lowest acid amount of NiP-700. Moreover, the catalyst deactivation was ascribed to carbon deposition on phosphates during the reaction.
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http://dx.doi.org/10.1166/jnn.2020.18873DOI Listing
December 2020

Astrocytic IGFBP2 and CHI3L1 in cerebrospinal fluid drive cortical metastasis of HER2+breast cancer.

Clin Exp Metastasis 2020 06 11;37(3):401-412. Epub 2020 Apr 11.

Division of Neurosurgery, Beckman Research Institute, City of Hope, 1500 E. Duarte Road, Duarte, CA, 91010, USA.

The brain is often reported as the first site of recurrence among breast cancer patients overexpressing human epidermal growth factor receptor 2 (HER2). Although most HER2+tumors metastasize to the subcortical region of the brain, a subset develops in the cortical region. We hypothesize that factors in cerebrospinal fluid (CSF) play a critical role in the adaptation, proliferation, and establishment of cortical metastases. We established novel cell lines using patient biopsies to model breast cancer cortical and subcortical metastases. We assessed the localization and growth of these cells in vivo and proliferation and apoptosis in vitro under various conditions. Proteomic analysis of human CSF identified astrocyte-derived factors that support the proliferation of cortical metastases, and we used neutralizing antibodies to test the effects of inhibiting these factors both in vivo and in vitro. The cortical breast cancer brain metastatic cells exhibited greater proliferation than subcortical breast cancer brain metastatic cells in CSF containing several growth factors that nourish both the CNS and tumor cells. Specifically, the astrocytic paracrine factors IGFBP2 and CHI3LI promoted the proliferation of cortical metastatic cells and the formation of metastatic lesions. Disruption of these factors suppressed astrocyte-tumor cell interactions in vitro and the growth of cortical tumors in vivo. Our findings suggest that inhibition of IGFBP2 and CHI3LI signaling, in addition to existing treatment modalities, may be an effective therapeutic strategy targeting breast cancer cortical metastasis.
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http://dx.doi.org/10.1007/s10585-020-10032-4DOI Listing
June 2020

Performance and microbial community of a novel combined anaerobic bioreactor integrating anaerobic baffling and anaerobic filtration process for low-strength rural wastewater treatment.

Environ Sci Pollut Res Int 2020 May 23;27(15):18743-18756. Epub 2020 Mar 23.

Beijing Engineering Research Center of Sustainable Urban Sewage System Construction and Risk Control, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.

A novel combined bioreactor integrating anaerobic baffling and anaerobic filtration process was developed and operated for 210 days to treat low-strength rural wastewater. The effects of hydraulic residence time (HRT) and organic loading rate (OLR) on chemical oxygen demand (COD) removal and methane (CH) production of the combined bioreactor were investigated. The combined bioreactor can start up successfully in 25 days and achieve enhanced performance. The COD removal rate and CH yield were influenced significantly by HRT and OLR. The influent COD was removed effectively through the synergistic effects of the anaerobic baffling and anaerobic filtration. The baffle zone played the main role in the degradation of the pollutants, and the filter zone mainly contributed to improve the resistance to shock loading. High-throughput sequencing technology was used to analyze the bacterial and archaeal community structure and diversity. Clostridium_sensu_stricto, Longilinea, Acetoanaerobium, Arcobacter, and Acinetobacter were found to be the dominant bacteria. While Methanothrix and Methanoregula were the dominant archaea, which were responsible for methane generation. This study not only highlights the good energy recovery and resource utilization potential of the combined bioreactor but also presents significant guidance for the application of the combined anaerobic process for low-strength rural wastewater treatment.
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http://dx.doi.org/10.1007/s11356-020-08263-9DOI Listing
May 2020

Targeted Sequencing and RNA Assay Reveal a Noncanonical Splicing Variant Causing Alagille Syndrome.

Front Genet 2019 24;10:1363. Epub 2020 Jan 24.

International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Alagille syndrome (ALGS), as known as congenital arteriohepatic dysplasia, is a rare autosomal dominant multi-systemic disorder. Mutations in or more rarely have been reported as the cause of ALGS. In this study, a 5-year old girl with typical ALGS feature and her pregnant mother came to our reproductive genetics clinic for counseling. We aimed to clarify the genetic diagnosis and provide prenatal genetic diagnosis for the pregnant. Next generation sequencing (NGS)-based multigene panel was used to identify pathogenic variant of the proband. Then the candidate variant was verified by using Sanger sequencing. RNA assay was performed to clarify splicing effect of the candidate variant. Amniocentesis, karyotyping, and Sanger sequencing were performed for prenatal testing. We found a novel noncanonical splicing variant (c.2917-8C > A) in the proband. Peripheral blood RNA assay suggested that the mutant transcript might escape nonsense-mediated messenger RNA (mRNA) decay (NMD) and encode a C-terminal truncated protein. Information of the variant has resulted in a successful prenatal diagnosis of the fetus. Our results clarified the genetic diagnosis of an ALGS patient and ensured utility of prenatal genetic testing.
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http://dx.doi.org/10.3389/fgene.2019.01363DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6993058PMC
January 2020

Comparative proteomic analysis of cucumber powdery mildew resistance between a single-segment substitution line and its recurrent parent.

Hortic Res 2019 15;6:115. Epub 2019 Oct 15.

School of Horticulture and Plant Protection, Yangzhou University, 225009 Yangzhou, Jiangsu China.

Powdery mildew (PM) is considered a major cause of yield losses and reduced quality in cucumber worldwide, but the molecular basis of PM resistance remains poorly understood. A segment substitution line, namely, SSL508-28, was developed with dominant PM resistance in the genetic background of PM-susceptible cucumber inbred line D8. The substituted segment contains 860 genes. An iTRAQ-based comparative proteomic technology was used to map the proteomes of PM-inoculated and untreated (control) D8 and SSL508-28. The number of differentially regulated proteins (DRPs) in SSL508-28 was almost three times higher than that in D8. Fourteen DRPs were located in the substituted segment interval. Comparative gene expression analysis revealed that nodulin-related protein 1 (NRP1) may be a good candidate for PM resistance. Gene Ontology enrichment analysis showed that DRPs functioning in tetrapyrrole biosynthetic process, sulfur metabolic process and cell redox homeostasis were specifically enriched in the resistant line SSL508-28. DRPs categorized in the KEGG term photosynthesis increased in both lines upon PM infection, suggesting that the strategies used by cucumber may be different from those used by other crops to react to PM attacks at the initial stage. The measurement of hydrogen peroxide and superoxide anion production and net photosynthetic rate were consistent with the changes in protein abundance, suggesting that the proteomic results were reliable. There was a poor correlation between DRPs measured by iTRAQ and the corresponding gene expression changes measured by RNA-seq with the same experimental design. Taken together, these findings improve the understanding of the molecular mechanisms underlying the response of cucumber to PM infection.
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http://dx.doi.org/10.1038/s41438-019-0198-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804742PMC
October 2019

Development of a High Stability Pd-Ni Alloy Thin-Film Coated SAW Device for Sensing Hydrogen.

Sensors (Basel) 2019 Aug 15;19(16). Epub 2019 Aug 15.

Key Laboratory of Non-Destructive Testing Ministry of Education, Nanchang HangKong University, Nanchang 330063, China.

A Pd-Ni alloy thin-film coated surface acoustic wave (SAW) device is proposed for sensing hydrogen. The Pd-Ni thin-film was sputtered onto the SAW propagation path of a SAW device with a delay line pattern to build the chip-sized hydrogen sensor. The prepared sensor chip was characterized by employing a differential oscillation loop. The effect of the Pd-Ni film thickness on sensing performance was also evaluated, and optimal parameters were determined, allowing for fast response and high sensitivity. Excellent working stability (detection error of 3.7% in half a year), high sensitivity (21.3 kHz/%), and fast response (less than 10 s) were achieved from the 40 nm Pd-Ni alloy thin-film coated sensing device.
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http://dx.doi.org/10.3390/s19163560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721225PMC
August 2019

Structure of the mannose transporter of the bacterial phosphotransferase system.

Cell Res 2019 08 17;29(8):680-682. Epub 2019 Jun 17.

State Key Laboratory of Membrane Biology, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, 100084, Beijing, China.

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http://dx.doi.org/10.1038/s41422-019-0194-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796895PMC
August 2019

Enhanced Delivery of Oncolytic Adenovirus by Neural Stem Cells for Treatment of Metastatic Ovarian Cancer.

Mol Ther Oncolytics 2019 Mar 13;12:79-92. Epub 2018 Dec 13.

Department of Stem Cell & Developmental Biology, City of Hope, 1500 East Duarte Road, Duarte, CA 91010, USA.

Oncolytic virotherapy is a promising approach for treating recurrent and/or drug-resistant ovarian cancer. However, its successful application in the clinic has been hampered by rapid immune-mediated clearance or neutralization of the virus, which reduces viral access to tumor foci. To overcome this barrier, patient-derived mesenchymal stem cells have been used to deliver virus to tumors, but variability associated with autologous cell isolations prevents this approach from being broadly clinically applicable. Here, we demonstrate the ability of an allogeneic, clonal neural stem cell (NSC) line (HB1.F3.CD21) to protect oncolytic viral cargo from neutralizing antibodies within patient ascites fluid and to deliver it to tumors within preclinical peritoneal ovarian metastases models. The viral payload used is a conditionally replication-competent adenovirus driven by the survivin promoter (CRAd-S-pk7). Because the protein survivin is highly expressed in ovarian cancer, but not in normal differentiated cells, viral replication should occur selectively in ovarian tumor cells. We found this viral agent was effective against cisplatin-resistant ovarian tumors and could be used as an adjunct treatment with cisplatin to decrease tumor burden without increasing toxicity. Collectively, our data suggest NSC-delivered CRAd-S-pk7 virotherapy holds promise for improving clinical outcome, reducing toxicities, and improving quality of life for patients with advanced ovarian cancer.
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http://dx.doi.org/10.1016/j.omto.2018.12.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350263PMC
March 2019

Understanding the Giant Gap between Single-Pore- and Membrane-Based Nanofluidic Osmotic Power Generators.

Small 2019 Mar 17;15(11):e1804279. Epub 2019 Jan 17.

CAS Key Laboratory of Bio-Inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.

Nanofluidic blue energy harvesting attracts great interest due to its high power density and easy-to-implement nature. Proof-of-concept studies on single-pore platforms show that the power density approaches up to 10 to 10 W m . However, to translate the estimated high power density into real high power becomes a challenge in membrane-scale applications. The actual power density from existing membrane materials is merely several watts per square meter. Understanding the origin and thereby bridging the giant gap between the single-pore demonstration and the membrane-scale application is therefore highly demanded. In this work, an intuitive resistance paradigm is adopted to show that this giant gap originates from the different ion transport property in porous membrane, which is dominated by both the constant reservoir resistance and the reservoir/nanopore interfacial resistance. In this case, the generated electric power becomes saturated despite the increasing pore number. The theoretical predictions are further compared with existing experimental results in literature. For both single nanopore and multipore membrane, the simulation results excellently cover the range of the experimental results. Importantly, by suppressing the reservoir and interfacial resistances, kW m to MW m power density can be achieved with multipore membranes, approaching the level of a single-pore system.
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http://dx.doi.org/10.1002/smll.201804279DOI Listing
March 2019

Image synthesis-based multi-modal image registration framework by using deep fully convolutional networks.

Med Biol Eng Comput 2019 May 7;57(5):1037-1048. Epub 2018 Dec 7.

Digital Medical Research Center, School of Basic Medical Sciences, Fudan University, Shanghai, China.

Multi-modal image registration has significant meanings in clinical diagnosis, treatment planning, and image-guided surgery. Since different modalities exhibit different characteristics, finding a fast and accurate correspondence between images of different modalities is still a challenge. In this paper, we propose an image synthesis-based multi-modal registration framework. Image synthesis is performed by a ten-layer fully convolutional network (FCN). The network is composed of 10 convolutional layers combined with batch normalization (BN) and rectified linear unit (ReLU), which can be trained to learn an end-to-end mapping from one modality to the other. After the cross-modality image synthesis, multi-modal registration can be transformed into mono-modal registration. The mono-modal registration can be solved by methods with lower computational complexity, such as sum of squared differences (SSD). We tested our method in T1-weighted vs T2-weighted, T1-weighted vs PD, and T2-weighted vs PD image registrations with BrainWeb phantom data and IXI real patients' data. The result shows that our framework can achieve higher registration accuracy than the state-of-the-art multi-modal image registration methods, such as local mutual information (LMI) and α-mutual information (α-MI). The average registration errors of our method in experiment with IXI real patients' data were 1.19, 2.23, and 1.57 compared to 1.53, 2.60, and 2.36 of LMI and 1.34, 2.39, and 1.76 of α-MI in T2-weighted vs PD, T1-weighted vs PD, and T1-weighted vs T2-weighted image registration, respectively. In this paper, we propose an image synthesis-based multi-modal image registration framework. A deep FCN model is developed to perform image synthesis for this framework, which can capture the complex nonlinear relationship between different modalities and discover complex structural representations automatically by a large number of trainable mapping and parameters and perform accurate image synthesis. The framework combined with the deep FCN model and mono-modal registration methods (SSD) can achieve fast and robust results in multi-modal medical image registration. Graphical abstract The workflow of proposed multi-modal image registration framework.
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http://dx.doi.org/10.1007/s11517-018-1924-yDOI Listing
May 2019

Enhanced Sensitivity of a Hydrogen Sulfide Sensor Based on Surface Acoustic Waves at Room Temperature.

Sensors (Basel) 2018 Nov 6;18(11). Epub 2018 Nov 6.

Institute of Acoustics, Chinese Academy of Sciences, Beijing 100190, China.

In this contribution, a new surface acoustic wave (SAW)-based sensor was proposed for sensing hydrogen sulfide (H₂S) at room temperature (30 °C), which was composed of a phase discrimination circuit, a SAW-sensing device patterned with delay line, and a triethanolamine (TEA) coating along the SAW propagation path of the sensing device. The TEA was chosen as the sensitive interface for H₂S sensing, owing to the high adsorption efficiency by van der Waals' interactions and hydrogen bonds with H₂S molecules at room temperature. The adsorption in TEA towards H₂S modulates the SAW propagation, and the change in the corresponding phase was converted into voltage signal proportional to H₂S concentration was collected as the sensor signal. A SAW delay line patterned on Y-cut quartz substrate with Al metallization was developed photographically, and lower insertion and excellent temperature stability were achieved thanks to the single-phase unidirectional transducers (SPUDTs) and lower cross-sensitivity of the piezoelectric substrate. The synthesized TEA by the reaction of ethylene oxide and ammonia was dropped into the SAW propagation path of the developed SAW device to build the H₂S sensor. The developed SAW sensor was characterized by being collecting into the phase discrimination circuit. The gas experimental results appear that fast response (7 s at 4 ppm H₂S), high sensitivity (0.152 mV/ppm) and lower detection limit (0.15 ppm) were achieved at room temperature. It means the proposed SAW sensor will be promising for H₂S sensing.
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http://dx.doi.org/10.3390/s18113796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263627PMC
November 2018

Catalytic dehydrogenative aromatization of cyclohexanones and cyclohexenones.

Org Biomol Chem 2018 11;16(45):8662-8676

College of Material and Chemical Engineering, Chuzhou University, Chuzhou, Anhui 239012, China.

Phenols and phenol derivatives serve as synthetic building blocks for the construction of compounds ranging from polymers to pharmaceuticals. Despite numerous methods for phenol synthesis, the catalytic dehydrogenation of carbocyclic compounds such as cyclohexanones and cyclohexenones to generate substituted phenol derivatives has received much attention. This review describes recent research progress concerning catalytic dehydrogenative aromatization and I2 promoted oxidative aromatization reactions in view of the types of cyclohexanone and cyclohexenone substrates and reactions, including the associated mechanisms and developments.
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http://dx.doi.org/10.1039/c8ob02351dDOI Listing
November 2018

Single molecule localization microscopy coupled with touch preparation for the quantification of trastuzumab-bound HER2.

Sci Rep 2018 10 11;8(1):15154. Epub 2018 Oct 11.

Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, CA, 91010, USA.

All breast cancers are assessed for levels of human epidermal growth factor receptor 2 (HER2). Fluorescence in situ hybridization (FISH) and immunohistochemistry are currently used to determine if a patient is eligible for anti-HER2 therapy. Limitations of both tests include variability and relatively long processing times. Additionally, neither test determines whether HER2 contains the extracellular domain. While truncated in some tumors, this domain is required for binding of the therapeutic antibody trastuzumab. Here, trastuzumab was used to directly detect HER2 with quantitative single molecule localization microscopy (qSMLM). In proof of concept studies, our new method rapidly quantified both HER2 density and features of nano-organization. In cultured cells, the method was sensitive to subtle variations in HER2 expression. To assess patient samples, we combined qSMLM with tissue touch preparation (touch prep-qSMLM) and examined large areas of intact membranes. For cell lines and patient samples, HER2 copy numbers from FISH showed a significant positive correlation with detected densities from qSMLM and trended with HER2 cluster occupancy.
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http://dx.doi.org/10.1038/s41598-018-33225-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181918PMC
October 2018

2'-Hydroxyflavanone effectively targets RLIP76-mediated drug transport and regulates critical signaling networks in breast cancer.

Oncotarget 2018 Apr 6;9(26):18053-18068. Epub 2018 Apr 6.

Department of Medical Oncology, Beckman Research Institute of City of Hope, Comprehensive Cancer Center and National Medical Center, Duarte, CA 91010, USA.

Breast cancer (BC) is the most common cancer in women. Estrogen, epidermal growth factor receptor 2 (ERBB2, HER2), and oxidative stress represent critical mechanistic nodes associated with BC. RLIP76 is a major mercapturic acid pathway transporter whose expression is increased in BC. In the quest of a novel molecule with chemopreventive and chemotherapeutic potential, we evaluated the effects of 2'-Hydroxyflavanone (2HF) in BC. 2HF enhanced the inhibitory effects of RLIP76 depletion and also inhibited RLIP76-mediated doxorubicin transport in BC cells. RNA-sequencing revealed that 2HF induces strong reversal of the gene expression pattern in ERMCF7, HER2 SKBR3 and triple-negative MDA-MB-231 BC cells with minimal effects on MCF10A normal breast epithelial cells. 2HF down regulated ERα and enhanced inhibitory effects of imatinib mesylate/Gleevec in MCF7 cells. 2HF also down regulated ERα and HER2 gene networks in MCF7 and SKBR3 cells, respectively. 2HF activated TP53 and inhibited TGFβ1 canonical pathway in MCF7 and MDA-MB-231 BC cells. 2HF also regulated the expression of a number of critical prognostic genes of MammaPrint panel and their upstream targets including TP53, CDKN2A and MYC. The collective findings from this study provide a comprehensive, direct and integrated evidence for the benefits of 2HF in targeting major and clinically relevant mechanistic regulators of BC.
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http://dx.doi.org/10.18632/oncotarget.24720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915057PMC
April 2018

Ammonium and arsenic trioxide are potent facilitators of oligonucleotide function when delivered by gymnosis.

Nucleic Acids Res 2018 04;46(7):3612-3624

Department of Medical Oncology and Experimental Therapeutics, City of Hope, 1500 E. Duarte Rd., Duarte, CA 91010, USA.

Oligonucleotide (ON) concentrations employed for therapeutic applications vary widely, but in general are high enough to raise significant concerns for off target effects and cellular toxicity. However, lowering ON concentrations reduces the chances of a therapeutic response, since typically relatively small amounts of ON are taken up by targeted cells in tissue culture. It is therefore imperative to identify new strategies to improve the concentration dependence of ON function. In this work, we have identified ammonium ion (NH4+) as a non-toxic potent enhancer of ON activity in the nucleus and cytoplasm following delivery by gymnosis. NH4+ is a metabolite that has been extensively employed as diuretic, expectorant, for the treatment of renal calculi and in a variety of other diseases. Enhancement of function can be found in attached and suspension cells, including in difficult-to-transfect Jurkat T and CEM T cells. We have also demonstrated that NH4+ can synergistically interact with arsenic trioxide (arsenite) to further promote ON function without producing any apparent increased cellular toxicity. These small, inexpensive, widely distributed molecules could be useful not only in laboratory experiments but potentially in therapeutic ON-based combinatorial strategy for clinical applications.
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http://dx.doi.org/10.1093/nar/gky150DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909461PMC
April 2018

Successive Waste as Reagent: Two More Steps Forward in a Pinnick Oxidation.

Org Lett 2018 02 2;20(4):913-916. Epub 2018 Feb 2.

Catalytic Hydrogenation Research Center, State Key Laboratory Breeding Base of Green Chemistry-Synthesis Technology, Zhejiang University of Technology , Hangzhou 310014, China.

The successful development of the classical Pinnick oxidation into a new and promising oxidative lactonization reaction is reported. Chiral 3-oxindolepropionic aldehydes, Michael adducts of 3-olefinic oxindoles with aliphatic aldehydes, are directly converted to spirocyclic oxindole-γ-lactones solely by sodium chlorite via a tandem Pinnick oxidation/chlorination/substitution sequence. This reaction uses waste ClO generated in the initial Pinnick oxidation as an ecofriendly halogenating agent for the subsequent chlorination, and then it utilizes the byproduct OH formed in the chlorination to facilitate the final internal substitution.
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http://dx.doi.org/10.1021/acs.orglett.7b03684DOI Listing
February 2018

Deformable multi-modal registration using 3D-FAST conditioned mutual information.

Comput Assist Surg (Abingdon) 2017 12 26;22(sup1):295-304. Epub 2017 Oct 26.

a Digital Medical Research Center , Fudan University , Shanghai , China.

Purpose: Mutual information (MI) has been a preferred choice of similarity measure for multi-modal image registration, but the accuracy and robustness of MI are not satisfactory as MI only considers the global intensity correlation while ignoring local and structural information. To address this problem, we combine MI with local and structural information.

Method: We bring structural information extracted by a modified Accelerated Segment Test (FAST) algorithm into MI. Traditional FAST is transferred into 3 D for the first time, and the 3 D-FAST based structural information is added into MI as another channel, thereby incorporating spatial and geometric information with intensity information in the registration.

Result: The robustness and accuracy of the proposed method were demonstrated in three experiments. The average registration errors of our method were 1.17, 1.33 and 1.20 compared to 1.47, 1.63 and 1.40 of LMI in T1-T2, T1-PD and T2-PD registration respectively.

Discussion: In this paper, we use the structural similarity computed by 3 D-FAST as the conditional information to encode spatial and geometric cues into LMI. In all of these three experiments, our method shows to be more robust and accurate than common registration methods based on information theory.
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http://dx.doi.org/10.1080/24699322.2017.1389408DOI Listing
December 2017

Different protein kinase C isoenzymes mediate inhibition of cardiac rapidly activating delayed rectifier K current by different G-protein coupled receptors.

Br J Pharmacol 2017 Dec 7;174(23):4464-4477. Epub 2017 Nov 7.

Department of Pharmacology, Hebei Medical University, Shijiazhuang, China; The Key Laboratory of New Drug Pharmacology and Toxicology, Shijiazhuang, China; The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, China.

Background And Purpose: Elevated angiotensin II (Ang II) and sympathetic activity contributes to a high risk of ventricular arrhythmias in heart disease. The rapidly activating delayed rectifier K current (I ) carried by the hERG channels plays a critical role in cardiac repolarization, and decreased I is involved in increased cardiac arrhythmogenicity. Stimulation of α -adrenoreceptors or angiotensin II AT receptors is known to inhibit I via PKC. Here, we have identified the PKC isoenzymes mediating the inhibition of I by activation of these two different GPCRs.

Experimental Approach: The whole-cell patch-clamp technique was used to record I in guinea pig cardiomyocytes and HEK293 cells co-transfected with hERG and α -adrenoreceptor or AT receptor genes.

Key Results: A broad spectrum PKC inhibitor Gö6983 (not inhibiting PKCε), a selective cPKC inhibitor Gö6976 and a PKCα-specific inhibitor peptide, blocked the inhibition of I by the α -adrenoreceptor agonist A61603. However, these inhibitors did not affect the reduction of I by activation of AT receptors, whereas the PKCε-selective inhibitor peptide did block the effect. The effects of angiotensin II and the PKCε activator peptide were inhibited in mutant hERG channels in which 17 of the 18 PKC phosphorylation sites were deleted, whereas a deletion of the N-terminus of the hERG channels selectively prevented the inhibition elicited by A61603 and the cPKC activator peptide.

Conclusions And Implications: Our results indicated that inhibition of I by activation of α -adrenoreceptors or AT receptors were mediated by PKCα and PKCε isoforms respectively, through different molecular mechanisms.
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http://dx.doi.org/10.1111/bph.14049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715974PMC
December 2017

Correlation Between Molecular Subclassifications of Clear Cell Renal Cell Carcinoma and Targeted Therapy Response.

Eur Urol Focus 2016 Jun 9;2(2):204-209. Epub 2015 Dec 9.

Department of Medical Oncology and Experimental Therapeutics, City of Hope Comprehensive Cancer Center, Duarte, CA, USA. Electronic address:

Background: Vascular endothelial growth factor (VEGF) and mammalian target of rapamycin (mTOR)-directed therapies are the standard of care in metastatic clear cell renal cell carcinoma (mccRCC) but are not used based on molecular subclassifications of ccRCC.

Objective: To determine if an association exists between genomic alterations (GAs) detected by comprehensive genomic profiling (CGP) in the course of clinical care and the response to anti-VEGF receptor (VEGFR) and anti-MTOR pathway targeted therapies in a cohort of patients with treated mccRCC.

Design, Setting, And Participants: CGP, using a Clinical Laboratory Improvement Amendments-certified platform, was performed on 31 formalin-fixed, paraffin-embedded tissue specimens (84% from cytoreductive nephrectomies) obtained from patients with metastatic renal cell carcinoma who had received VEGFR and/or mTOR inhibitors. Duration of treatment (DOT) and extent and duration of clinical response were obtained from review of medical records.

Outcome Measurements And Statistical Analysis: All classes of GAs-base substitutions, short insertions, deletions, gene fusions, rearrangements, and copy number-were assessed via hybrid capture-based CGP. Descriptive statistics were used to determine the frequency of GAs in groups segregated by the DOT with VEGF-directed agents.

Results And Limitations: The most common GAs detected in this series were in VHL (70%), PBRM1 (48%), SETD2 (32%), TSC1 (29%), MLL (19%), TERT (16%), ARID1B (16%), and KDM5C (16%). Across 61 administrations of VEGF-directed therapy in 27 patients, exceptional responses (DOT >21 mo) were more frequent among patients with GAs in KDM5C, PBRM1, and VHL. Conversely, these patients also featured a lower frequency of GA associated with response to mTOR-directed therapy, such as TSC1.

Conclusions: Molecular subclassifications may affect response to VEGF-directed therapy. The predictive and prognostic nature of these molecular subclassifications in the metastatic setting should be explored in an extended series.

Patient Summary: Comprehensive genomic profiling in the course of clinical care in the community oncology setting can delineate subgroups of patients with advanced kidney cancer who stand to benefit more from specific molecular-targeted agents.
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http://dx.doi.org/10.1016/j.euf.2015.11.007DOI Listing
June 2016