BSc, MSc, PhD
Hebei University and Beijing Institute of Technology
Beijing, Beijing | China
Additional Specialties: Molecular Genetics, Molecular Biology
Primary Affiliation: Hebei University and Beijing Institute of Technology - Beijing, Beijing , China
PubMed Central Citations
28PubMed Central Citations
Chin Med J 2017;130:460-9
Chinese Medical Journal
Background: The calcium clearance and reactive oxygen species (ROS) generations in the coronary artery smooth muscle cells in chronic heart failure (HF) have not been fully investigated. Therefore, we attempted to understand the gene expressions underlying the mishandling of calcium clearance and the accumulations of ROS. Methods: We initially established an animal model of chronic HF by making the left anterior descending coronary artery ligation (CAL) in rats, and then isolated the coronary artery vascular smooth muscle cells from the ischemic and the nonischemic parts of the coronary artery vessels in 12 weeks after CAL operation. The intracellular calcium concentration and ROS level were measured using flow cytometry, and the gene expressions of sarco/endoplasmic reticulum Ca2+‑ATPase (SERCA2a), encoding sarcoplasmic reticulum Ca2+‑ATPase 2a, encoding sodium‑calcium exchanger (NCX), and p47phox encoding a subunit of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase were examined using real‑time quantitative reverse transcription polymerase chain reaction and Western blotting, respectively. Results: We found that the calcium accumulation and ROS generation in the coronary artery smooth muscle cells isolated from either the ischemic or the nonischemic part of the CAL coronary artery vessel were significantly increased irrespective of blood supply (all P < 0.01). Moreover, these were accompanied by the increased expressions of NCX and p47phox, the decreased expression of SERCA2a, and the increased amount of phosphorylated forms of p47phox in NADPH oxidase (all P < 0.05). Conclusions: Our results demonstrated that the disordered calcium clearance and the increased ROS generation occurred in the coronary artery smooth muscle cells in rats with chronic HF produced by ligation of the left anterior descending coronary artery (CAL), and which was found to be disassociated from blood supply, and the increased generation of ROS in the cells was found to make concomitancy to the increased activity of NADPH oxidase in cytoplasm.
Iran J Basic Med Sci 2016 Nov;19(11):1216-1221
School of Basic Medical Sciences, Hebei University, Baoding 071002, China; School of Life Science, Beijing Institute of Technology, Beijing 10081, China.
Journal of Clinical and Laboratory Investigation Updates
DNA inverted repeats are abundant in all genomes with potential of adopting DNA hairpin and cruciform structures that sometimes raise genomic instabilities. This work showed that an inverted repeat DNA sequence in bovine prochymosin gene could only be cloned in M13 bacteriophage by one orientation, and propagation of this orientation in E.coli cells raised an immediate deletion of a 102-bp DNA fragment, containing partial repeat DNA and partial M13 DNA. The deletion was found to be mediated by two tandemly arranged “CTGC” sequences, one in the inverted repeat sequence and another in the M13 phage, respectively, indicating that the deletion occurred via a nascent strand slipped mis-pairing replication mechanism. We were failed in cloning the opposite orientation of the inverted repeats by screening a large number of white bacteriophage plagues, suggesting that orientation may be inviable. By contrast, the same inverted repeats can well be cloned and propagated in a pUC19 plasmid in E.coli with two orientations. These results suggested that the maintenance of the inverted repeat in vivo was tightly related to the repeat foldability subjected to its location in a DNA sequence and its replication modes used.
Progress in Natural Science
RecR protein, a functional equivalent of Rad52 in eukaryotes, plays a critical role in the RecF pathway of homologous recombination in Escherichia coli. By constructing and expressing the recR-yfp hybrid gene, the distribution of the RecR-YFP fusion protein was visualized in E. coli by fluorescent microscopy. Our results showed that RecR proteins can be localized predominantly in the nucleoid region of E. coli. By measuring the UV resistance of a recR mutant carrying the recR-yfp gene in the plasmid, the expressed RecR-YFP was found to be functional in improving the UV resistance of the recR deficiency strain.
Acta Genetica Sinica (Journal of Genetics and Genomics)
Effects of degU32 (Hy), degR genes from Bacillus subtilis 168 and degQa gene from Bacillus amyloliquefaciens on Bacillus subtilis Ki-2-132 cell growth, sporulation and protease fermentation were investigated by introducing these genes into B. subtilis Ki-2-132 chromosome and/or cytoplasm. Although the genes come from different species and strains, they showed pleiotropic effects in B. subtilis Ki-2-132. B. subtilis Ki-2-132degU32 (Hy) showed increased protease production, and when cooperating with degQa either in plasmid or in chromosome, further altered cell growth, increased protease production and affected the spore formation in a glucose and dosage dependent manner. By contrast, degR did not significantly affect the protease productivity in degU32 (Hy) mutant, consisting with that DegR was used to stabilise DegU-phosphate, which in degU32 (Hy) strain no longer further amplify the DegU-phosphate effect.
Eur J Biochem 2004 Sep;271(18):3665-70
Institute of Microbiology, The Chinese Academy of Sciences, Beijing, China.
Microbiol Res 2004 ;159(2):97-102
Institute of Microbiology, Chinese Academy of Sciences, Academy of Science, Beijing 100080, China.
Nucleic Acid Res
A 24 triplet TGG·CCA repeat array shows length- and orientation-dependent propagation when present in the plasmid pUC18. When TGG24 is present as template for leading-strand synthesis, plasmid recovery is normal in all strains tested. However, when it acts as template for lagging-strand synthesis, plasmid propagation is seriously compromised. Plasmids carrying deletions in the 5′ side of this sequence can be isolated and products carrying 15 TGG triplets do not significantly interfere with plasmid propagation. Mutations in sbcCD, mutS and recA significantly improve the recovery of plasmids with TGG24 on the lagging-strand template. These findings suggest that TGG24 can fold into a structure that can interfere with DNA replication in vivo but that TGG15 cannot. Furthermore, since the presence of the MutS and SbcCD proteins are required for propagation interference, it is likely that stabilisation of mismatched base pairs and secondary structure cleavage are implicated. In contrast, there is no correlation of triplet repeat expansion and deletion instability with predicted DNA folding. These results argue for a dissociation of the factors affecting DNA fragility from those affecting trinucleotide repeat expansion–contraction instability.