Publications by authors named "Xue-Qing Wu"

33 Publications

In Silico Screening of Potential Spike Glycoprotein Inhibitors of SARS-CoV-2 with Drug Repurposing Strategy.

Chin J Integr Med 2020 Sep 1;26(9):663-669. Epub 2020 Aug 1.

Clinical Medical Academy, Shenzhen University, Shenzhen, Guangdong, 518055, China.

Objective: To select potential molecules that can target viral spike proteins, which may potentially interrupt the interaction between the human angiotension-converting enzyme 2 (ACE2) receptor and viral spike protein by virtual screening.

Methods: The three-dimensional (3D)-coordinate file of the receptor-binding domain (RBD)-ACE2 complex for searching a suitable docking pocket was firstly downloaded and prepared. Secondly, approximately 15,000 molecular candidates were prepared, including US Food and Drug Administration (FDA)-approved drugs from DrugBank and natural compounds from Traditional Chinese Medicine Systems Pharmacology (TCMSP), for the docking process. Then, virtual screening was performed and the binding energy in Autodock Vina was calculated. Finally, the top 20 molecules with high binding energy and their Chinese medicine (CM) herb sources were listed in this paper.

Results: It was found that digitoxin, a cardiac glycoside in DrugBank and bisindigotin in TCMSP had the highest docking scores. Interestingly, two of the CM herbs containing the natural compounds that had relatively high binding scores, Forsythiae fructus and Isatidis radix, are components of Lianhua Qingwen (), a CM formula reportedly exerting activity against severe acute respiratory syndrome (SARS)-Cov-2. Moreover, raltegravir, an HIV integrase inhibitor, was found to have a relatively high binding score.

Conclusions: A class of compounds, which are from FDA-approved drugs and CM natural compounds, that had high binding energy with RBD of the viral spike protein. Our work provides potential candidates for other researchers to identify inhibitors to prevent SARS-CoV-2 infection, and highlights the importance of CM and integrative application of CM and Western medicine on treating COVID-19.
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http://dx.doi.org/10.1007/s11655-020-3427-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395204PMC
September 2020

Healthy at 100 Through Lifestyle Medicine.

Am J Med 2019 08;132(8):e663

University Department of Obstetrics and Gynecology, First Affiliated Hospital, Medical University of Wenzhou, Zhejiang, China.

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http://dx.doi.org/10.1016/j.amjmed.2019.02.030DOI Listing
August 2019

[Simultaneous Nitrification and Denitrifying Phosphorus Removal in Continuous Flow Reactor with Intermittent Aeration].

Huan Jing Ke Xue 2019 Feb;40(2):799-807

School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.

A continuous flow reactor (TCFR) with 10 compartments was used to treat domestic sewage. The anaerobic compartments of TCFR were kept at 3. The anoxic compartments of TCFR were reduced from 2 to 0. Therefore, the aerobic compartments of TCFR were increased gradually from 5 to 7. The aerobic compartments were set to continual aeration in Run1 and intermittent aeration from Run2 to Run4. The aeration/non-aeration ratios were 40 min/20 min,40 min/30 min, and 40 min/40 min, respectively. The nitrification liquid reflux ratios were reduced gradually from 150% to 0%. When the average influent concentrations of COD, NH-N, TN, and PO-P were 259.34, 60.26, 64.42, and 6.10 mg·L, respectively, the corresponding effluent concentrations were 26.40, 1.03, 5.84, and 0.3 mg·L, respectively in Run4. The nitrogen removal amounts increased gradually from 192.30 mg·h in Run1 to 244.00 mg·h in Run4, and the corresponding removal rates increased from 65.40% to 95.30%. The activity of denitrifying phosphorus accumulating organisms (DPAOs) and phosphorus accumulating organisms (PAOs) increased from 36.05% and 38.20% in Run1 to 140.50% and 133.40% in Run4, respectively. Simultaneous nitrification and denitrifying phosphorus removal was achieved in TCFR by adopting intermittent aeration, which provided a reference for the reformation of sewage treatment plants.
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http://dx.doi.org/10.13227/j.hjkx.201807093DOI Listing
February 2019

Metformin Promotes Regeneration of the Injured Endometrium Via Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis.

Reprod Sci 2019 04 22;26(4):560-568. Epub 2018 Nov 22.

1 Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Intrauterine adhesion (IUA) is now recognized as one of the most common diseases in reproductive-age women. Metformin, a well-known frontline oral antidiabetic drug, has been found effective in numerous different diseases. The aim of this study was to determine the effect of metformin on reducing adhesions in an animal model of IUA. Sprague-Dawley rats were randomized into 4 groups: sham operation, control, metformin-treated for 7 days, and metformin-treated for 14 days. To establish the IUA model, mechanical injury to the endometria of rats was induced with a mini curette. Metformin was injected intraperitoneally after surgery. A significant amelioration in both the number of glands and the fibrotic area, compared to those of the control group, was detected 14 days after metformin intervention. The expression levels of antigen KI-67 and vascular endothelial growth factor were increased at 7 and 14 days after treatment. However, the transforming growth factor-β expression was decreased at 14 days after treatment. Endoplasmic reticulum stress-related apoptosis proteins (glucose-regulated protein 78, caspase-12, and CCAAT/enhancer binding protein (EBP) homologous protein) were downregulated after metformin treatment. Moreover, we determined that the effect of metformin was related to the inhibition of endoplasmic reticulum stress-induced apoptosis via the Phosphatidylinositol 3 kinase (PI3K)/Protein kinase B (AKT) and Extracellular regulated protein kinases1/2 pathways. In conclusion, metformin can attenuate the adhesion and promote the regeneration of the endometrium of the IUA rat, and metformin may serve as a novel therapeutic strategy for IUA patients.
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http://dx.doi.org/10.1177/1933719118804424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421622PMC
April 2019

Purinergic, P2X3 positive cells in amniotic membranes.

Am J Obstet Gynecol 2019 01 29;220(1):125-126. Epub 2018 Sep 29.

Department of Obstetrics and Gynaecology, Medical University of Wenzhou, 1 Nanbaixiang Road, Ouhai, Wenzhou, People's Republic of China 325000. Electronic address:

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http://dx.doi.org/10.1016/j.ajog.2018.09.032DOI Listing
January 2019

Low-frequency repetitive transcranial magnetic stimulation inhibits the development of methamphetamine-induced conditioned place preference.

Behav Brain Res 2018 11 9;353:129-136. Epub 2018 Jul 9.

Collaborative Innovation Center for Brain Science, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Psychotic Disorders, Shanghai, China. Electronic address:

The abuse of amphetamine-type stimulants (ATS) has become a global public health issue in recent years, these new-type drugs can cause addiction and serious cognitive impairment. However, there are no effective methods for the prevention and treatment of ATS addiction at present. Repetitive transcranial magnetic stimulation (rTMS) is a painless and non-invasive new therapeutic approach that has been used for the treatment of depression and other neuropsychiatric disorders, but whether it can be used to treat drug addiction is unclear. In the present study, we investigated the possible effects of rTMS on methamphetamine(METH)-induced conditioned place preference (CPP). High-frequency (10 Hz) and low-frequency stimulation patterns (1 Hz) were applied to test the effect of rTMS on METH-induced CPP. The results showed that low-frequency but not high-frequency rTMS could block METH-CPP, accompanied with a downregulation of gamma-aminobutyric acid type B receptor subunit 1 (GABAR1) expression in rat dorsolateral striatum. These results suggested that low-frequency rTMS could effectively inhibit the development of METH addiction and shed light on the rTMS as a potential approach for the prevention of drug addiction.
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http://dx.doi.org/10.1016/j.bbr.2018.07.004DOI Listing
November 2018

Re: Pre-eclampsia is primarily a placental disorder: The arteriolar injury in pre-eclampsia.

BJOG 2018 03 14;125(4):511-512. Epub 2017 Dec 14.

Department of Obstetrics & Gynaecology, First Affiliated Hospital, Medical University of Wenzhou, Wenzhou, Zhejiang, China.

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http://dx.doi.org/10.1111/1471-0528.15008DOI Listing
March 2018

The effect of neonatal immune challenge on reproduction by altering intraovarian kisspeptin/GPR54 system in the rat.

Reprod Toxicol 2017 12 21;74:40-47. Epub 2017 Sep 21.

Department of Obstetrics and Gynecology, Shenzhen University General Hospital, Guangdong, China; Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address:

Immune challenge in early life has been observed to influence the long-term reproductive dysfunction. On PNDs 3 and 5, female offsprings were administered with LPS (50μg/kg, i.p.) or saline. Vaginal opening was recorded, and oestrous cyclicity was monitored immediately post puberty and again at 56-70 days. At 10 weeks of age, the ovaries were removed for immunostaining and RNA analysis. Neonatal exposure to LPS resulted in a significant delay puberty onset as well as destroyed expression of ovulation related genes. At PND 42 and 70, a significant increase in Kiss1 mRNA and Kisspeptin expression was detected at proestrus and oestrus in neo-LPS treated rats compared with the counterparts. Therefore, neonatal LPS exposure had a long-term effect on reproductive function and the up-regulated expression of ovarian Kiss1 and kisspeptin during the ovulatory transition stage may contribute to ovulatory dysfunction induced by peripheral LPS administration in early life.
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http://dx.doi.org/10.1016/j.reprotox.2017.08.021DOI Listing
December 2017

Three-dimensional structure micelles of heparin-poloxamer improve the therapeutic effect of 17β-estradiol on endometrial regeneration for intrauterine adhesions in a rat model.

Int J Nanomedicine 2017 7;12:5643-5657. Epub 2017 Aug 7.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.

Intrauterine adhesions (IUA) frequently occur after infectious or mechanical injury to the endometrium, which may lead to infertility and/or pregnancy complications. There are few effective treatments due to the complex function of endometrium and shortage of native materials. 17β-estradiol (E) is commonly used as an ancillary treatment in IUA patients, but it is limited by its poor solubility in aqueous solutions and low concentrations at the injured sites. In this research, a mini-endometrial curette was used to injure the rat's endometrium to form an IUA model. 17β-estradiol was encapsulated into the micelles of heparin-poloxamer and a thermosensitive hydrogel (E-HP hydrogel) was formed. This sustained releasing system was applied to restore the structure and function of the injured uterus. E-HP hydrogel was constructed and relevant characteristics including gelation temperature and micromorphology were evaluated. Sustained release of 17β-estradiol from HP hydrogel was performed both in vitro and in vivo. Ultrasonography measurement and pathologic characteristics on the IUA rats were performed to evaluate the therapeutic effect of E-HP hydrogel. Endoplasmic reticulum (ER) stress-related apoptosis was analyzed to explore the possible mechanisms in IUA recovery. E-HP hydrogel showed a prolonged release of E at the targeting region and more effective endometrium regeneration in IUA rats. Significant improvements in both gland numbers and fibrosis area were observed in the E-HP hydrogel group. We also demonstrated that E-HP hydrogel in the recovery of IUA was closely related to the suppression of ER stress signals via the activation of downstream signals, PI3K/Akt and ERK1/2. HP hydrogel might be an effective approach to deliver E into the injured endometrium. Therapeutic strategies targeting ER stress using E-HP hydrogel might be a promising solution for the treatment of women with intrauterine adhesions.
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http://dx.doi.org/10.2147/IJN.S137237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557621PMC
January 2018

Dual Regulations of Thermosensitive Heparin-Poloxamer Hydrogel Using ε-Polylysine: Bioadhesivity and Controlled KGF Release for Enhancing Wound Healing of Endometrial Injury.

ACS Appl Mater Interfaces 2017 Sep 24;9(35):29580-29594. Epub 2017 Aug 24.

Department of Pharmaceutics, School of Pharmaceutical Sciences and ‡First Affiliated Hospital, Wenzhou Medical University , Wenzhou City, Zhejiang Province 325035, China.

Hydrogel was not only used as an effective support matrix to prevent intrauterine adhesion after endometrial injury but also served as scaffold to sustain release of some therapeutics, especially growth factor. However, because of the rapid turnover of the endometrial mucus, the poor retention and bad absorption of therapeutic agents in damaged endometrial cavity were two important factors hindering their pharmacologic effect. Herein, a mucoadhesive hydrogel was described by using heparin-modified poloxamer (HP) as the matrix material and ε-polylysine (EPL) as functional excipient. Various EPL-HP hydrogels formulations are screened by rheological evaluation and mucoadhesion studies. It was found that the rheological and mucoadhesive properties of EPL-HP hydrogels were easily controlled by changing the amount of EPL in formulation. The storage modulus of EPL-HP hydrogel with 90 μg/mL of EPL (EPL-HP-90) was elevated to be 1.9 × 10 Pa, in accordance with the adhesion force rising to 3.18 N (10-fold higher than HP hydrogels). Moreover, in vitro release of model drug keratinocyte growth factor (KGF) from EPL-HP hydrogel was significantly accelerated by adding EPL in comparison with HP hydrogel. Both strong mucoadhesive ability and the accelerated drug release behavior for EPL-HP-90 made more of the encapsulated KGF absorbed by the uterus basal layer and endometrial glands after 8 h of administration in uterus cavity. Meanwhile, the morphology of endometrium in the injured uterus was repaired well after 3 d of treatment with KGF-EPL-HP-90 hydrogels. Compared with KGF-HP group, not only proliferation of endometrial epithelial cell and glands but also angiogenesis in the regenerated endometrium was obviously enhanced after treatment with KGF-EPL-HP-90 hydrogels. Alternatively, the cellular apoptosis in the damaged endometrium was significantly inhibited after treatment with KGF-EPL-HP-90 hydrogels. Overall, the mucoadhesive EPL-HP hydrogel with a suitable KGF release profile may be a more promising approach than HP hydrogel alone to repair the injured endometrium.
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http://dx.doi.org/10.1021/acsami.7b10211DOI Listing
September 2017

Temperature-sensitive heparin-modified poloxamer hydrogel with affinity to KGF facilitate the morphologic and functional recovery of the injured rat uterus.

Drug Deliv 2017 Nov;24(1):867-881

a Department of Pharmaceutics, School of Pharmaceutical Sciences , Wenzhou Medical University , Wenzhou City , PR China.

Endometrial injury usually results in intrauterine adhesion (IUA), which is an important cause of infertility and recurrent miscarriage in reproductive women. There is still lack of an effective therapeutic strategy to prevent occurrence of IUA. Keratinocyte growth factor (KGF) is a potent repair factor for epithelial tissues. Here, a temperature-sensitive heparin-modified poloxamer (HP) hydrogel with affinity to KGF (KGF-HP) was used as a support matrix to prevent IUA and deliver KGF. The rheology of KGF-HP hydrogel was carefully characterized. The cold KGF-HP solution was rapidly transited to hydrogel with suitable storage modulus (G') and loss modulus (G″) for the applications of uterus cavity at temperature of 33 °C. In vitro release demonstrated that KGF was released from HP hydrogels in sustained release manner for a long time. In vivo bioluminescence imaging showed that KGF-HP hydrogel was able to prolong the retention of the encapsulated KGF in injured uterus of rat model. Moreover, the morphology and function of the injured uterus were significantly recovered after administration of KGF-HP hydrogel, which were evaluated by two-dimensional ultrasound imaging and receptive fertility. Not only proliferation of endometrial glandular epithelial cells and luminal epithelial cells but also angiogenesis of injured uterus were observed by Ki67 and CD31 staining after 7 d of treatment with KGF-HP hydrogel. Finally, a close relatively relationship between autophagy and proliferation of endometrial epithelial cells (EEC) and angiogenesis was firstly confirmed by detecting expression of LC3-II and P62 after KGF treatment. Overall, KGF-HP may be used as a promising candidate for IUA treatment.
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http://dx.doi.org/10.1080/10717544.2017.1333173DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241134PMC
November 2017

The aetiology of pre-eclampsia, 1945-1953.

BJOG 2016 Dec;123(13):2130

First Affiliated Hospital, Medical University of Wenzhou, Wenzhou, China.

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http://dx.doi.org/10.1111/1471-0528.14297DOI Listing
December 2016

All-Cause and Cause-Specific Mortality After Hypertensive Disease of Pregnancy.

Obstet Gynecol 2016 12;128(6):1445-1446

First Affiliated Hospital, Wenzhou, China.

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http://dx.doi.org/10.1097/AOG.0000000000001789DOI Listing
December 2016

Medical evacuation of the uterus and subsequent preterm labor.

Am J Obstet Gynecol 2016 12 26;215(6):809-810. Epub 2016 Jul 26.

Department of Obstetrics and Gynecology, First Affiliated Hospital, Medical University of Wenzhou, Wenzhou, China. Electronic address:

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http://dx.doi.org/10.1016/j.ajog.2016.07.047DOI Listing
December 2016

The effects of small litter rearing on ovarian function at puberty and adulthood in the rat.

Reprod Biol 2016 Jun 18;16(2):130-7. Epub 2016 Feb 18.

Department of Gynecology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People's Republic of China; Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London, Guy's Campus, 2.92W, Hodgkin Building, London SE1 1UL, UK. Electronic address:

Rearing rats in small litters lead to obesity and reproductive dysfunction. We investigated the effects of rearing female rats in small litters on various reproductive parameters during puberty and into adulthood, and examined the possible involvement of local ovarian sympathetic nerve activity. The litter size was adjusted on postnatal day one to four pups per dam for the small litters and 12 pups per dam for the normal litters. Vaginal opening was recorded, and estrous cyclicity was monitored daily immediately post puberty for 14 days and again at 8-9 weeks of age. At the time of puberty and 10 weeks of age, the ovaries were collected. The number of different types of follicles was counted and the thickness of the theca interna of the largest antral follicles was measured. Ovarian sympathetic nerve activity was assessed immunohistochemically by measuring levels of ovarian nerve growth factor receptor (p75NGFR) and tyrosine hydroxylase (TH). In rats reared in small litters, there was a significant advancement of puberty and disruption of estrous cyclicity immediately post puberty. The number of antral follicles increased in the small litter reared rats at puberty compared with their controls. The thickness of the theca interna increased and the expression profiles of ovarian p75NGFR and TH increased in small litter reared rats at puberty, but this did not persist into adulthood. These data suggest that rearing rats in small litters lead to irregular reproductive cycles, which might involve increased local ovarian sympathetic nerve activity.
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http://dx.doi.org/10.1016/j.repbio.2016.02.001DOI Listing
June 2016

Menopausal Symptoms and Sleep Quality During Menopausal Transition and Postmenopause.

Chin Med J (Engl) 2016 Apr;129(7):771-7

Gynecology and Obstetrics Center, Shanxi Women and Children's Hospital, Taiyuan, Shanxi 030013, China.

Background: Menopausal symptoms and sleep difficulty were physiological processes that were affected by genetic and other factors. This study was to investigate the prevalence of menopausal symptoms and sleep quality in menopausal transition (MT) and postmenopause (PM) women in Taiyuan, Shanxi.

Methods: A community-based survey of women's menopausal symptoms and sleep quality was conducted between July 2012 and May 2013 at six municipal districts of Taiyuan, Shanxi. A sample of 2429 women aged 40-59 years was divided into four groups: early MT, late MT, early PM, and late PM. Sleep quality in the past 2 weeks before the interview was recorded. The data were analyzed using SPSS 16.0.

Results: The prevalence of menopausal symptoms was 49.8%. Mild, moderate, and severe symptoms were observed in 28.9%, 18.5%, and 2.5% of participants, respectively. The highest prevalence of menopausal symptoms occurred in the early postmenopausal stage; the subsequences were the late postmenopausal stage and the early MT stage. Interestingly, among the 13 items of modified Kupperman index, the five most common symptoms were fatigue, arthralgia and myalgia, decreased libido, insomnia, and nervousness. Meanwhile, 55% perimenopausal women had poor sleep.

Conclusions: Menopausal symptoms are common but mild among women in Taiyuan, Shanxi during MT and PM. In these stages, the prevalence of poor sleep is high.
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http://dx.doi.org/10.4103/0366-6999.178961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819295PMC
April 2016

Villoglandular papillary adenocarcinoma of the uterine cervix: A report of 4 cases and a review of the literature.

Oncol Lett 2016 Jan 18;11(1):837-841. Epub 2015 Nov 18.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.

To investigate the clinicopathological features, management and prognosis of villoglandular papillary adenocarcinoma (VGPA) of the uterine cervix, the current study presents 4 cases of VGPA of the uterine cervix. The median age of the patients was 55 years (range, 47-70 years), with all 4 patients presenting with stage Ib disease. Human papillomavirus (HPV) infection was detected in 3 patients; this was mainly HPV-16. No history of oral contraceptive use was found in these cases. While 2 of the patients underwent a radical hysterectomy with bilateral salpingo-oophorectomy plus bilateral pelvic lymphadenectomy, 1 patient underwent a radical hysterectomy with bilateral pelvic lymphadenectomy and the remaining patient received a simple total hysterectomy plus post-operative radiotherapy. Of these patients, only 1 had been correctly diagnosed pre-operatively. In 2 patients, the biopsy results had been interpreted as cervical adenocarcinoma, and in the third, the biopsy result was of cervical intraepithelial neoplasia. All 4 patients presented with cervical wall invasion, including invasion of the inner two-thirds in 1 patient. No lymphovascular space invasion or lymph node metastasis was detected. The follow-up time ranged from 49 to 83 months (median, 64 months), and the patients are currently alive and well, with no evidence of recurrent disease. Taking these results as a whole, VGPA is an uncommon type of cervical adenocarcinoma, characterized by its excellent prognosis. HPV infection is associated with the molecular pathogenesis of VGPA, while oral contraceptive use can be excluded. As the disease has a low pre-operative diagnostic accuracy, frequent cervical wall invasion and concomitant lesions, conservative treatment strategies should be carefully considered.
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http://dx.doi.org/10.3892/ol.2015.3944DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727193PMC
January 2016

Association between FSHR polymorphisms and polycystic ovary syndrome among Chinese women in north China.

J Assist Reprod Genet 2014 Mar 5;31(3):371-7. Epub 2014 Jan 5.

Center of Reproductive Medicine, Shanxi Women and Children Health Hospital, No. 13, Xin Min Bei Jie, Xinghualing District, 030013, Taiyuan, Shanxi, China,

Purpose: Polycystic ovary syndrome (PCOS) is a common endocrine disorder disease among women in reproductive-age. Since follicle stimulating hormone (FSH) exerts important biological functions, the association between PCOS and FSH receptor (FSHR) polymorphisms attracts wide attention. The aim of this study was to evaluate whether polymorphisms of FSHR at 307 and 680 codons are associated with PCOS patients in China.

Methods: Patients with PCOS (n = 215) and controls (n = 205) were recruited from Shanxi Province in north China. They are Han ethnics. Genomic DNA was isolated from the venous blood. The Ala307Thr and Ser680Asn polymorphisms of FSHR were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct DNA sequencing.

Results: The distributions of genotype and allele of Ala307Thr and Ser680Asn polymorphisms of FSHR were not statistically different between the PCOS patients and the controls. Analysis of the frequency of FSHR polymorphisms showed no statistical difference among the PCOS patients with different obesity standards. Although there were no statistical differences in the most of the endocrine parameters including LH, LH/FSH, E2, P and T as well as the clinical pregnancy rate, there were significant differences in the levels of FSH and PRL among PCOS patients carrying different genotypes of Ala307Thr and Ser680Asn polymorphisms.

Conclusion: The Ala307Thr and Ser680Asn polymorphisms of FSHR are not associated with PCOS in Han ethnic Chinese women in north China. The FSHR polymorphisms was related to the levels of FSH and PRL but not other PCOS-associated endocrine hormones as well as clinical pregnancy rate in PCOS patients of Han Chinese ethnical population.
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http://dx.doi.org/10.1007/s10815-013-0166-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947065PMC
March 2014

Risk assessment of nitrate and oxytetracycline addition on coastal ecosystem functions.

Aquat Toxicol 2014 Jan 7;146:76-81. Epub 2013 Nov 7.

Department of Chemistry & Environmental Science, Minnan Normal University, Zhangzhou 363000, China.

Diatoms dominate phytoplankton communities in the well-mixed coastal and upwelling regions. Coastal diatoms are often exposed to both aquaculture pollution and eutrophication. But how these exposures influence on coastal ecosystem functions are unknown. To examine these influences, a coastal centric diatom, Conticribra weissflogii was maintained at different concentrations of nitrate (N) and/or oxytetracycline (OTC). Algal density, cell growth cycle, protein, chlorophyll a, superoxide dismutase (SOD) activity, and malonaldehyde (MDA) were determined for the assessment of algal biomass, lifetime, nutritional value, photosynthesis and respiration, antioxidant capacity, and lipid peroxidation, respectively. When N addition was combined with OTC pollution, the cell growth cycles were shortened by 56-73%; algal density, SOD activities, the concentrations of chlorophyll a, protein, and MDA varied between 73 and 121%, 19 and 397%, 52 and 693%, 19 and 875%, and 66 and 2733% of the values observed in N addition experiments, respectively. According to P-value analysis, the influence of OTC on algal density and SOD activity was not significant, but the effect on cell growth cycle, protein, chlorophyll a, and MDA were significant (P<0.05). The influence of N addition with simultaneous OTC pollution on the above six end points was significant. Algal biomass, lifetime, nutrition, antioxidant capacity, lipid peroxidation, photosynthesis, and respiration were all affected by the addition of OTC and N. Coastal ecosystem functions were severely affected by N and OTC additions, and the influence was increased in the order: N
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http://dx.doi.org/10.1016/j.aquatox.2013.10.028DOI Listing
January 2014

[Effects on the secretion of reproductive hormones in adult female rats exposed to lipopolysaccharide in neonatal life].

Zhonghua Yi Xue Za Zhi 2013 Jun;93(21):1674-6

Department of Obstetrics & Gynecology; Department of Cardiology, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.

Objective: To explore whether neonatal endotoxin challenge alters reproductive hormone secretion in adult female rats.

Methods: Rats were intraperitoneally injected with lipopolysaccharide (LPS) or normal saline at postnatal days 3 and 5. At Week 12, the animals underwent bilateral oophorectomy and were exposed to LPS or saline. The secretion levels of luteinizing hormone (LH) and estradiol were measured.

Results: The LPS injection in adult neonatal saline rats caused the suppressed secretion of LH and estradiol. However, the decreased secretion of both LH and estradiol were much more apparent in adult neonatal LPS-treated rats.

Conclusion: The neonatal LPS challenge alters the secretions of LH and estradiol during adulthood. And the mechanism may be mediated by epigenetic programming of hypothalamic-pituitary-gonadal axis.
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June 2013

[Effects of ru'ai shuhou recipe on the matrix metalloproteinases and the inhibitive factors in the recurrence and metastasis of HER2 positive breast cancer].

Zhongguo Zhong Xi Yi Jie He Za Zhi 2012 Nov;32(11):1526-30

Department of Breast Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai.

Objective: To observe the anti-tumor recurrent and metastatic efficacy of Ru'ai Shuhou Recipe (RSR) on HER2 positive breast cancer, to evaluate the effects of RSR on the expressions of matrix metalloproteinases (MMPs) and the tissue inhibitor of metalloproteinases (TIMPs) in the recurrence and metastasis of HER2 positive breast cancer, thus revealing its anti-tumor recurrent and metastatic mechanisms.

Methods: Selected were 30-week-old HER2/neu transgenic spontaneous breast cancer mice FVB/neu. The primary tumor resection was carried out. After surgery they were randomly divided into the blank control group, the RSR group, the Herceptin group, and the combination group (RSR + Herceptin group). The treatment lasted for 4 months. The inhibition rate of the recurrent tumor volume and the inhibition rate of the lung metastasis were evaluated. The expressions of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase (TIMP-1), and TIMP-2 in the recurrent tumor tissue were detected using Western blot.

Results: By the end of the treatment the average recurrent tumor volume was 11.11 +/- 8.71 cm3 in the blank control group and 5.56 +/- 5.55 cm3 of the RSR group, showing statistical difference between the two groups (P = 0.037). The average lung metastatic nodule was 16 in the blank control group and 10 in the RSR group. The inhibition rate of lung metastasis was 37. 85% in the RSR group, but with no statistical significance. The expression level of activated MMP-2 in the RSR group was down-regulated when compared with the blank control group, the Herceptin group, and the combination group (P < 0.05). The expression of MMP-9 of the RSR group, the Herceptin group, and the combination group was significantly down-regulated when compared with the blank control group (P < 0.05). The expression of MMP-9 of the RSR group and the combination group was further down-regulated when compared with the Herceptin group (P < 0.05). The expressions of both TIMP-1 and TIMP-2 of the RSR group, the Herceptin group, and the combination group were all up-regulated when compared with the blank control group (P < 0.05). The increased expression of TIMP-1 was more significantly in the RSR group and the combination group when compared with the Herceptin group (P < 0.05). It was higher in the combination group than in the RSR group (P < 0.05).

Conclusions: RSR could inhibit the tumor recurrence of FVB/neu mice. It could reduce the degradation of extracellular matrix and increase the protective effects of extracellular matrix. It might achieve its anti-tumor effect through effecting the invasive and metastatic capabilities of breast tumor cells.
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November 2012

[Effects of obesity on the lipid and glucose metabolism in female rats].

Zhonghua Yi Xue Za Zhi 2012 Apr;92(13):932-4

First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.

Objective: To investigate the effects of obesity on the lipid and glucose metabolism in female rats.

Methods: The litter size was adjusted to 4 pups per litter for the small litter (SL, treatment group) and 12 neonates per dam in the normal litter (NL, controls) at postnatal day 0. Body weights were measured weekly. At the age of 6 weeks, bilateral ovariectomy was performed. The blood levels of free fat acid, triglyceride, total cholesterol, free blood glucose and free insulin were evaluated. The muscular expression of AMP-activated protein kinase was detected by immune staining.

Results: As compared to normal litters, the levels of fat acid, triglyceride, total cholesterol, free blood glucose and free insulin significantly increased. The muscular activity of AMP-activated protein kinase decreased. All difference were statistically significant (P < 0.05).

Conclusion: Obesity in early life has obvious effects on the lipid and glucose metabolism in female rats.
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April 2012

[A comparison of ultrasonography versus traditional approach for caudal block in children].

Zhonghua Yi Xue Za Zhi 2012 Apr;92(13):882-5

Department of Anesthesiology, Tianjin Children's Hospital, Tianjin 300074, China.

Objective: To apply ultrasonic technology in positioning and monitoring for pediatric caudal blocks and compare it with traditional landmark approach and Swoosh test.

Methods: After obtaining the approval of hospital ethics committee and written parental informed consent, a total of 102 American Society of Anesthesiologists (ASA) I-II pediatric patients aged from 1 month to 8 years and scheduled for urologic or perineal surgery were recruited. Patients were randomly divided into 2 groups: ultrasound group (n = 52) and control group (n = 50). The sites of sacral cornu and sacral hiatus were determined by ultrasonic imaging or classic method of anatomical surface landmarks. Patients of two groups were punctured according to the scheduled site. Local anesthetic was injected slowly into caudal space after a successful caudal puncture and the positive reaction in caudal space was monitored simultaneously by ultrasound and classic swoosh test. The observational results and relevant date were recorded.

Results: In ultrasound and control groups, the number of puncture attempts was 1.10 ± 0.30 vs 1.56 ± 0.63, the duration of puncture (1.40 ± 0.39) vs (3.23 ± 1.23) min, the success rate at the first puncture attempt 90.4% vs 66% and the total puncture success rate 100% vs 92% respectively. After the injection of local anesthetic, the positive reaction rate of ultrasonography and swoosh test was 97.96% vs 62.24% respectively. All of above results in ultrasound group were superior to those in control group or with classic test. And the difference had statistical significance (P < 0.01).

Conclusion: Ultrasonic positioning and monitoring for pediatric caudal block is both scientific and reasonable. The positioning detected by ultrasonic imaging is accurate and the monitoring results of ultrasound are reliable. It is obviously superior to traditional method and has clinical application values for caudal block in children.
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April 2012

[Influence on pubertal reproductive function in female rats by immune challenge in early life].

Zhonghua Fu Chan Ke Za Zhi 2011 Jun;46(6):441-5

Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical College, China.

Objective: To investigate the long-term programming effects on pubertal reproductive function by immunological challenge in early life.

Methods: Female Sprague-Dawley rats were administered by endotoxin (lipopolysaccharide, LPS) at a dosage of 50 µg/kg and saline intraperitoneally on postnatal day 3 and 5. Body weight was measured weekly. Puberty onset (vaginal opening) and oestrous cyclicity were monitored from postnatal day 30. At the age of 6 weeks, bilateral ovariectomy was performed. The histological and morphological change of the ovaries (the thickness of the theca interna and the number of different kinds of follicles) were observed and the immunoreactivity of the ovarian sympathetic nerve markers (low affinity receptor of nerve growth factor, p75NGFR) was evaluated by immune staining.

Results: Immunological challenge (exposed to LPS) in early life delayed vaginal opening significantly [LPS-treated (40.6 ± 0.7) days versus controls (38.6 ± 0.5) days, P < 0.05], decreased the percentage of normal oestrous cyclicity (LPS-treated 26.1% versus controls 66.8%, P < 0.05), decreased the total number of different types of follicles (primordial follicles: LPS-treated 610 ± 47 versus controls 1181 ± 57, P < 0.05; primary follicles: LPS-treated 624 ± 41 versus controls 960 ± 30, P < 0.05; preantral follicles: LPS-treated 183 ± 16 versus controls 260 ± 14, P < 0.05; antral follicles: LPS-treated 32 ± 4 versus controls 79 ± 7, P < 0.05) and increased the thickness of the theca interna [LPS-treated (15.8 ± 0.4) µm versus controls (11.4 ± 0.3) µm, P < 0.05]. The immunostaining of p75NGFR was obviously enhanced in the LPS-treated ovaries when compared with that of controls (P < 0.05).

Conclusions: Immunological stress during early critical developmental windows could have long dysfunctional effects on the pubertal reproductive function. It delayed puberty onset, reduced the percentage of the normal oestrous cycles, decreased follicles reserve and increased the thickness of the theca interna which might involve the up-regulation of the local ovarian sympathetic nerve activity.
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June 2011

Neonatal programming by immunological challenge: effects on ovarian function in the adult rat.

Reproduction 2011 Feb 17;141(2):241-8. Epub 2010 Nov 17.

Division of Women's Health, School of Medicine, King's College London, Guy's Campus, 2.95W, Hodgkin Building, London SE1 1UL, UK.

Neonatal exposure to an immunological challenge (lipopolysaccharide, LPS) increases the activity of hypothalamo-pituitary-adrenal axis and sensitises the GNRH pulse generator to the inhibitory influence of stress in adult rats. We investigated the effects of neonatal exposure to LPS on various reproductive parameters during puberty and into adulthood in female rats. LPS (50 μg/kg, i.p.) or saline was administered on postnatal days 3 and 5. Vaginal opening was recorded, and oestrous cyclicity was monitored immediately post puberty and again at 8-9 weeks of age. At 10 weeks of age, the ovaries were removed and the number of follicles was counted, together with the thickness of the theca interna of the largest antral follicles. Ovarian sympathetic nerve activity was assessed immunohistochemically by measurement of the levels of ovarian low-affinity receptor of nerve growth factor (p75NGFR). In rats exposed to LPS in early life, there was a significant delay in puberty and disruption of oestrous cyclicity immediately post puberty, which persisted into adulthood. The follicle reserve was decreased, the thickness of the theca interna increased and the expression profile of ovarian p75NGFR increased in the neonatal LPS-treated animals. These data suggest that exposure to LPS during early neonatal life can have long-term dysfunctional effects on the female reproductive system, which might involve, at least in part, increased ovarian sympathetic nerve activity.
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http://dx.doi.org/10.1530/REP-10-0252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021912PMC
February 2011

[Effect of ru'ai shuhou recipe on immune response in HER2/neu tranagenic mice undergoing breast cancer carcinogenesis process].

Zhongguo Zhong Xi Yi Jie He Za Zhi 2010 Jul;30(7):717-9, 756

Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai.

Objective: To explore the immune response induced by HER2/neu oncogene in the breast cancer (BC) carcinogenesis process and the immunological mechanism of Ru'ai Shuhou Recipe (RSR) in the prevention and treatment of BC.

Methods: HER2/neu transgenic spontaneous breast tumor model mice were fed with RSR from 5 weeks old, the occurrence of breast tumor in them was observed, and the changes of T cell-mediated immune response and associated cytokines were detected during the carcinogenesis process, i. e., when mice aged between 15 and 25 weeks.

Results: RSR showed significant effects in postponing and reducing the carcinogenesis of primary breast tumor, up-regulating the amount of T cell in splenic lymphocyte in tumor-bearing mice, promoting the proliferation of T lymphocyte, and inducing the secretion of cytokines such as interleukin-2, interleukin-12 and interferon-y.

Conclusions: A serial immune response reveals in the carcinogenesis process. The immunologic function of HER-2/neu transgenic mice is significantly different to that of the same strain non-transgenic mice. Effect of RSR in preventing and postponing breast cancer carcinogenesis is possibly realized through enhancing the anti-tumor immune response of transgenic mice themselves.
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July 2010

Translocation of classical PKC and cortical granule exocytosis of human oocyte in germinal vesicle and metaphase II stage.

Acta Pharmacol Sin 2006 Oct;27(10):1353-8

Institute of Urology, Peking University, Beijing 100034, China.

Aim: Protein kinase C (PKC) is as a family of serine/threonine kinases that can be activated by Ca2+, phospholipid and diacylglycerol. PKC plays an important role in oocyte maturation and activation. This study was undertaken to investigate classical PKC (cPKC) in human oocyte maturation and activation.

Methods: Germinal vesicle (GV) and metaphase II (MII) stage oocytes were collected from healthy women. The expression and distribution of cPKC were investigated by immunoflourescence. MII oocytes were treated with PKC activator or inhibitor and imaged using a laser confocal scanning microscope (LCSM).

Results: In GV oocytes, PKCalpha, beta1 and gamma were localized to the germinal vesicles, with a weak expression in ooplasm. In MII oocytes, PKCalpha, beta1 and gamma were distributed evenly in ooplasm. After treatment with PKC activator, phorbol 12-myristate 13-acetate (PMA), cPKC translocated to the periphery of oocyte, and cortical granules (CG) exocytosis was found. When the oocytes were treated with PKC inhibitor, staurosporine, no translocation of cPKC and CG exocytosis were found.

Conclusion: PKCalpha, beta1 and gamma exist in human oocytes and activation of these subunits could induce CG exocytosis in MII stage.
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http://dx.doi.org/10.1111/j.1745-7254.2006.00407.xDOI Listing
October 2006

Deficit of mitochondria-derived ATP during oxidative stress impairs mouse MII oocyte spindles.

Cell Res 2006 Oct 19;16(10):841-50. Epub 2006 Sep 19.

Peking University First Hospital, Institute of Urology of Peking University, Beijing 100034, China.

Although the role of oxidative stress in maternal aging and infertility has been suggested, the underlying mechanisms are not fully understood. The present study is designed to determine the relationship between mitochondrial function and spindle stability in metaphase II (MII) oocytes under oxidative stress. MII mouse oocytes were treated with H2O2 in the presence or absence of permeability transition pores (PTPs) blockers cyclosporin A (CsA). In addition, antioxidant N-acetylcysteine (NAC), F0/F1 synthase inhibitor oligomycin A, the mitochondria uncoupler carbonyl cyanide 4-trifluoro-methoxyphenylhydrazone (FCCP) or thapsigargin plus 2.5 mM Ca2+ (Th+2.5 mM Ca2+) were used in mechanistic studies. Morphologic analyses of oocyte spindles and chromosomes were performed and mitochondrial membrane potential (DeltaPsim), cytoplasmic free calcium concentration ([Ca2+]c) and cytoplasmic ATP content within oocytes were also assayed. In a time- and H2O2 dose-dependent manner, disruption of meiotic spindles was found after oocytes were treated with H2O2, which was prevented by pre-treatment with NAC. Administration of H2O2 led to a dissipation of DeltaPsim, an increase in [Ca2+]c and a decrease in cytoplasmic ATP levels. These detrimental responses of oocytes to H2O2 treatment could be blocked by pre-incubation with CsA. Similar to H2O2, both oligomycin A and FCCP dissipated DeltaPsim, decreased cytoplasmic ATP contents and disassembled MII oocyte spindles, while high [Ca2+]c alone had no effects on spindle morphology. In conclusion, the decrease in mitochondria-derived ATP during oxidative stress may cause a disassembly of mouse MII oocyte spindles, presumably due to the opening of the mitochondrial PTPs.
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http://dx.doi.org/10.1038/sj.cr.7310095DOI Listing
October 2006
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