Publications by authors named "Xue-Ping Li"

24 Publications

  • Page 1 of 1

Rehabilitation and pharmacotherapy of neuromyelitis optica spectrum disorder: A case report.

World J Clin Cases 2021 Jun;9(16):3951-3959

Department of Rehabilitation Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu Province, China.

Background: Neuromyelitis optica spectrum disorder (NMOSD) is a demyelinating autoimmune disease that affects the central nervous system. It typically manifests as optic neuritis or extensive longitudinal myelitis, with or without the presence of anti-aquaporin protein 4 autoantibodies (immunoglobulin G).

Case Summary: We report the case of a 45-year-old woman with a history of Sjogren's syndrome who was diagnosed with NMOSD accompanied by spinal cord injury and left calf intermuscular vein thrombosis. The patient received hormone shock and gamma globulin therapy in the acute phase and standard rehabilitation treatment during convalescence. Upon discharge, the patient was able to control urination and defecation, stand independently, and walk short distances with the aid of a walker.

Conclusion: This case suggests that pharmacotherapy and standard rehabilitation treatment can improve the prognosis of NMSOD patients.
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http://dx.doi.org/10.12998/wjcc.v9.i16.3951DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180214PMC
June 2021

SARS-CoV-2-related IFITM3 in immune dysfunction and tumor microenvironment: An integrative analysis in pan-cancers.

Clin Transl Med 2021 02;11(2):e345

Department of Hematology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

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http://dx.doi.org/10.1002/ctm2.345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901722PMC
February 2021

Bone marrow infiltrated natural killer cells predicted the anti-leukemia activity of MCL1 or BCL2 inhibitors in acute myeloid leukemia.

Mol Cancer 2021 01 5;20(1). Epub 2021 Jan 5.

National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.

Acute myeloid leukemia (AML) is still incurable due to its heterogeneity and complexity of tumor microenvironment. It is imperative therefore to understand the molecular pathogenesis of AML and identify leukemia-associated biomarkers to formulate effective treatment strategies. Here, we systematically analyzed the clinical characters and natural killer (NK) cells portion in seventy newly-diagnosis (ND) AML patients. We found that the proportion of NK cells in the bone marrow of ND-AML patients could predict the prognosis of patients by analyzing the types and expression abundance of NK related ligands in tumor cells. Furthermore, MCL1 inhibitor but not BCL2 inhibitor combined with NK cell-based immunotherapy could effectively improve the therapeutic efficiency via inhibiting proliferation and inducing apoptosis of AML primary cells as well as cell lines in vitro. There results provide valuable insights that could help for exploring new therapeutic strategies for leukemia treatment.
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http://dx.doi.org/10.1186/s12943-020-01302-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784307PMC
January 2021

Multidimensional study of the heterogeneity of leukemia cells in t(8;21) acute myelogenous leukemia identifies the subtype with poor outcome.

Proc Natl Acad Sci U S A 2020 08 3;117(33):20117-20126. Epub 2020 Aug 3.

State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China;

t(8;21)(q22;q22) acute myelogenous leukemia (AML) is morphologically characterized by a continuum of heterogeneous leukemia cells from myeloblasts to differentiated myeloid elements. Thus, t(8;21) AML is an excellent model for studying heterogeneous cell populations and cellular evolution during disease progression. Using integrative analyses of immunophenotype, RNA-sequencing (RNA-seq), and single-cell RNA-sequencing (scRNA-seq), we identified three distinct intrapatient leukemic cell populations that were arrested at different stages of myeloid differentiation: CD34CD117 blasts, CD34CD117 blasts, and abnormal myeloid cells with partial maturation (AM). CD117 is also known as c-KIT protein. CD34CD117 cells were blocked in the G0/G1 phase at disease onset, presenting with the regular morphology of myeloblasts showing features of granulocyte-monocyte progenitors (GMP), and were drug-resistant to chemotherapy. Genes associated with cell migration and adhesion (, , and ) were highly expressed in the CD34CD117 population. CD34CD117 blasts were blocked a bit later than the CD34CD117 population in the hematopoietic differentiation stage and displayed high proliferation ability. AM cells, which bear abnormal myelocyte morphology, especially overexpressed granule genes , , and and were sensitive to chemotherapy. scRNA-seq at different time points identified CD34CD117 blasts as an important leukemic cluster that expanded at postrelapse refractory stage after several cycles of chemotherapy. Patients with (8;21) AML with a higher proportion of CD34CD117 cells had significantly worse clinical outcomes than those with a lower CD34CD117 proportion. Univariate and multivariate analyses identified CD34CD117 proportion as an independent factor for poor disease outcome. Our study provides evidence for the multidimensional heterogeneity of t(8;21)AML and may offer new tools for future disease stratification.
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http://dx.doi.org/10.1073/pnas.2003900117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443908PMC
August 2020

Bioinformatics analysis reveals novel core genes associated with nonalcoholic fatty liver disease and nonalcoholic steatohepatitis.

Gene 2020 Jun 14;742:144549. Epub 2020 Mar 14.

Xi'an Medical University, Xi'an, China. Electronic address:

Nonalcoholic fatty liver disease (NAFLD) is the most frequent liver disease and associated with a wide spectrum of hepatic disorders ranging from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH), cirrhosis, and hepatocellular carcinoma (HCC). NASH is projected to become the most common indication for liver transplantation, and the annual incidence rate of NASH-related HCC is 5.29 cases per 1000 person-years. Owing to the epidemics of NAFLD and the unclear mechanism of NAFLD progression, it is important to elucidate the underlying NAFLD mechanisms in detail. NASH is mainly caused by the development of NAFL Therefore, it is also of great significance to understand the mechanism of progression from NAFL to NASH. Gene expression chip data for NAFLD and NASH were downloaded from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) between NAFLD and normal controls (called DEGs for NAFLD), as well as between NASH and normal tissue (called DEGs for NASH-Normal), and between NASH and NAFL tissue (called DEGs for NASH-NAFL). For DEGs for the NAFLD group, key genes were identified by studying the form of intersection. Potential functions of DEGs for NASH were then analyzed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. A protein-protein interaction network (PPI) was constructed using the STRING database. A total of 249 DEGs and one key gene for NAFLD were identified. For NASH-Normal, 514 DEGs and 11 hub genes were identified, three of which were closely related to the survival analysis of HCC, and potentially closely related to progression from NASH to HCC. One key gene for NASH-NAFL (AKR1B10) was identified. These genes appear to mediate the molecular mechanism underlying NAFLD and may be promising biomarkers for the presence of NASH.
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http://dx.doi.org/10.1016/j.gene.2020.144549DOI Listing
June 2020

Change of Anatomical Location of the Internal Carotid Artery Relative to the Atlas with Congenital Occipitalization and the Relevant Clinical Implications.

World Neurosurg 2019 Oct 27;130:e505-e512. Epub 2019 Jun 27.

Department of Neurosurgery, Chinese PLA General Hospital, Beijing, China. Electronic address:

Introduction: The occipitalization of the atlas (OA) is always associated with multiplanar dislocation and olisthy of the C1 over C2 facets, which may change the anatomical relationship between the internal carotid artery (ICA) and the atlas. The purpose of this current study is to identify the location of the ICA relative to the anterior aspect of the atlas in patients with OA and define the clinical implications for screw placement.

Methods: We retrospectively reviewed the computed tomography angiography data of 86 patients with OA and 86 control subjects. Several parameters were also measured to quantitatively evaluate the mutual relationship.

Results: In the OA group, 25.6% of ICAs were located in area 3 and 74.4% in area 2, whereas the percentages were 57.4% and 42.6%, respectively, in the control group. There were 73 (42.4%) ICAs in which the shortest distance between the dorsal surface of the ICA and the ventral cortex of the atlas was less than 4 mm in the OA group and only 50 (29.1%) in the control group. The ideal angulation of C1 screw trajectory was about 5 degrees more medial in the OA group than that in the control group (P < 0.01).

Conclusions: The risk of ICA injury is much higher in OA patients than in non-OA patients during the C1 screw placement. A mean medial angulation about 20 degrees will permit a long and safe screw purchase, but should be individualized. We recommend careful preoperative computed tomography angiography evaluation in all patients before surgery.
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http://dx.doi.org/10.1016/j.wneu.2019.06.138DOI Listing
October 2019

A simpler diagnostic formula for screening nonalcoholic fatty liver disease.

Clin Biochem 2019 Feb 29;64:18-23. Epub 2018 Nov 29.

Xi'an Medical University, Xi'an, China. Electronic address:

Objective: To increase the accuracy of non-invasive diagnosis of nonalcoholic fatty liver disease (NAFLD), clinical and laboratory NAFLD indicators were integrated into a diagnostic formula.

Methods: A total of 141 patients with clinically diagnosed NAFLD and 30 healthy controls were enrolled. We collected case history, body weight, height and mass index (BMI), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transpeptidase, blood urea nitrogen and blood uric acid (UA), serum creatinine, plasma total cholesterol, triglyceride, low density lipoprotein, glycosylated hemoglobin, fasting plasma glucose, fasting insulin, ultrasonic tests, Fibroscans, and other data. Linear correlation, multiple linear regressions, and receiver operating characteristic (ROC) curve methods were used to process and analyze the collected data. The performance of Fibroscan and our diagnostic formula was compared in reference to the findings of liver biopsy.

Results: The identified NAFLD diagnostic indices consisted of BMI, ALT, AST and UA. A regression formula was proposed as: CAP = 113.163 + 0.252 * ALT + 6.316 * BMI. Diagnosis of the area under the ROC curve was 0.927, the sensitivity was 87.68%, and specificity was 90%. The cutoff was 277.67 (p < 0.01). The accuracy of the NAFLD diagnosis with the proposed formula was significantly higher than FibroScan (82.6% vs 69.6%; p = 0.005).

Conclusions: NAFLD diagnosis with the proposed formula demonstrated both high sensitivity and specificity, and its accuracy was significantly higher than FibroScan. This formula only utilized non-invasive clinical and laboratory findings and the calculation was simple. It can be conveniently used for clinical diagnosis of NAFLD.
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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.011DOI Listing
February 2019

UV-Resistant and Thermally Stable Superhydrophobic CeO Nanotubes with High Water Adhesion.

Small 2018 Jul 3;14(27):e1801040. Epub 2018 Jun 3.

College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.

A novel type of sticky superhydrophobic cerium dioxide (CeO ) nanotube material is prepared by hydrothermal treatment without any chemical modification. A water droplet on the material surface shows a static water contact angle of about 157° but the water droplet is pinned on the material surface even when the material surface is turned upside down. Interestingly, the as-prepared CeO nanotube material displays durable superhydrophobicity and enhanced adhesion to water under ultraviolet (UV) light irradiation. Importantly, this change in water adhesion can be reversed by heat treatment to restore the original adhesive value of 20 µL. Further, the maximum volume of the water droplet adhered on the material surface of CeO nanotubes can be regulated without loss of superhydrophobicity during the heating treatment/UV-irradiation cycling. Meanwhile, the superhydrophobic CeO nanotube material shows remarkable thermal stability even at temperatures as high as 450 °C, long-term durability in chemical environment, and air-storage and good resistance to oily contaminant. Finally, the potential application in no-loss water transportation of this sticky superhydrophobic CeO material is demonstrated.
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http://dx.doi.org/10.1002/smll.201801040DOI Listing
July 2018

[Change of ion content in Phyllostachys vivax and Ph. glauca and its relationships with growth and photosynthesis in coastal regions].

Ying Yong Sheng Tai Xue Bao 2016 Oct;27(10):3145-3152

International Centre for Bamboo and Rattan, Beijing 100102, China.

The changes of Na, K, Ca, Mgcontents of 10 year-old Phyllostachys vivax and Ph. glauca and the relationships with the growth and photosynthesis were investigated in the shelter belt of coastal beach with three salt levels, i.e., 0.1% of mild salinity zone, 0.2% of moderate salinity zone, and 0.4% of severe salinity zone. Compared with the mild salinity zone, bamboo density and diameter of Ph. vivax growing in the severe salinity zone decreased by 30.4% and 28.8%, and such reductions were 44.1% and 31.2% for Ph. glauca, respectively. Salinity decreased the biomass of the two species, and the decrease of biomass in shoots was greater than that in underground organs. Compared with the mild salinity zone, the net photosynthetic rate (P) and the maximum PS2 photochemical efficiency (F/F) of Ph. vivax and Ph. glauca decreased by 57.6% and 67.7%, and 6.1% and 7.4% in the severe salinity zone, respectively. Ph. vivax had stronger salt-tolerance than Ph. glauca in saline environment. Na contents in roots, rhizome, stems, and leaves were significantly elevated in Ph. vivax and Ph. glauca with increasing soil salinity, however, the contents of K, Ca and Mg were reduced. Na was markedly accumulated in roots, and K was generally enriched in shoots. Salinity led to an evident decline of Ca in Ph. vivax roots and Mg in Ph. glauca leaves. The biomass, P and F/F of the two species were usually negatively correlated to Na content, and positively correlated to K and Ca contents.
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http://dx.doi.org/10.13287/j.1001-9332.201610.007DOI Listing
October 2016

κ Opioid receptor activation in different brain regions differentially modulates anxiety-related behaviors in mice.

Neuropharmacology 2016 11 20;110(Pt A):92-101. Epub 2016 Apr 20.

Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica and Collaborative Innovation Center for Brain Science, Chinese Academy of Science, Shanghai 201203, China. Electronic address:

κ Opioid receptor system is widely implicated in the regulation of emotion. However, the findings about the role on anxiety in rodents are highly controversial, since both anxiogenic- and anxiolytic-like effects have been reported with κ opioid receptor activation. The mechanism and the underlying neuroanatomical substrates are unexplored. In the present study, we first investigated the effects of κ agonist U50,488H on anxiety-related behaviors over a wide range of doses, and we found that U50,488H produced dual effects in anxiety, with low dose being anxiogenic and high dose being anxiolytic. To assess the potential neuroanatomical substrates, we used phosphorylation of extracellular signal-related kinase1/2 (pERK1/2) to map the underlying neural circuits. We found that the anxiogenic effect of U50,488H was paralleled by an increase of pERK1/2 in the nucleus accumbens, whereas the anxiolytic effect was paralleled by an increase of pERK1/2 in the lateral septal nucleus. We then examined the behavioral consequences with locally microinjection of U50,488H, and we found that microinjection of U50,488H into the nucleus accumbens exerted anxiogenic-like effects, whereas microinjection of U50,488H into the lateral septal nucleus. Both effects can be abolished by κ antagonist nor-BNI pretreatment. To the best of our knowledge, the present work firstly provides the neuroanatomical sites that mediating the dual anxiogenic- and anxiolytic-like effects of U50,488H in mice. This study may help to explain current controversial role of κ receptor activation in anxiety-related behaviors in rodents, and may open new perspectives in the areas of anxiety disorders and κ receptor function.
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http://dx.doi.org/10.1016/j.neuropharm.2016.04.022DOI Listing
November 2016

[Effects of Fe-Cd interaction on the lipid peroxidation and antioxidative enzyme activities of rice].

Ying Yong Sheng Tai Xue Bao 2013 Aug;24(8):2179-85

National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China.

Taking rice variety Shennong 265 as test material, a hydroponic experiment was conducted to investigate the effects of Fe (0, 0.1, 0.25 and 0.5 mmol Fe2+ x L(-1)) and Cd (0, 0.1 and 1.0 umol Cd2+ x L(-1)) on the lipid peroxidation and antioxidative enzyme activities of rice plant. When the Fe was supplied alone, the shoot and root dry mass decreased significantly, but this phenomenon would not occur when the Cd was applied simultaneously. Applying Cd alone decreased the root malondialdehyde (MDA) and soluble protein contents, but applying Fe simultaneously alleviated the negative effects of Cd. Applying Fe decreased the Cd concentrations in shoots and roots, whereas applying Cd decreased the shoot and root Fe concentrations, indicating an obvious antagonistic interaction between Fe and Cd. The interaction of high concentration (1.0 micromol x L(-1)) Cd with Fe increased the root MDA and soluble protein contents, and decreased the root superoxide dismutase (SOD) and catalase (CAT) activities. These results indicated that applying definite amount of exogenous Fe could decrease the Cd accumulation in rice under low Cd stress, whereas high Cd stress would decrease the Fe absorption by rice and induce the lipid peroxidation in rice plant.
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August 2013

Long-term fertilisation causes excess supply and loss of phosphorus in purple paddy soil.

J Sci Food Agric 2014 Apr 21;94(6):1175-83. Epub 2013 Oct 21.

College of Resources and Environment, Southwest University, Chongqing, 400716, P.R. China.

Background: Phosphorus (P) loss from cropland is accelerating the eutrophication of waters around the world such as the Three Gorges Reservoir (TGR) in China. We investigated whether purple paddy soil under long-term P applications was a major source of P efflux to the TGR.

Results: Substantial surplus P in the plough layer (0-20 cm) was evident after 15-year P applications. Available P (Olsen-P) in the plough layer ranged from 1.9 to 42.4 mg kg(-1) and some of which will exceed the threshold of 30 mg kg(-1) for environmental concern within 7 years of P application (inorganic NPK with or without organic fertiliser). Between 30 and 70% of Olsen-P was leached out of the 0-30 cm soil layer. Surplus P resulted in high P concentrations in the surface water during the rice cropping season, and these concentrations exceeded those in most waters of the TGR and exceeded the critical level for eutrophication (0.1 mg L(-1)) during the first 10 days after rice planting. Furthermore, total P in run-off due to rainfall events exceeded the level for eutrophication, with a total loss of 43.2-147.9 g P ha(-1) depending on the fertilisation.

Conclusion: Current agronomic P management in purple paddy soil is environmentally unsustainable in terms of the adverse impact on surface water quality. Integrated P management practices are urgently required to optimise crop yield while minimising P loss in order to protect surface water quality in the TGR region.
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http://dx.doi.org/10.1002/jsfa.6390DOI Listing
April 2014

Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening.

J Exp Bot 2012 Sep 9;63(14):5171-87. Epub 2012 Aug 9.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Horticultural Science South China Agricultural University, Guangzhou 510642, PR China.

The plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) play important roles in plant growth, development, and stress responses. However, the precise role of NAC TFs in relation to fruit ripening is poorly understood. In this study, six NAC genes, designated MaNAC1-MaNAC6, were isolated and characterized from banana fruit. Subcellular localization showed that MaNAC1-MaNAC5 proteins localized preferentially to the nucleus, while MaNAC6 was distributed throughout the entire cell. A transactivation assay in yeast demonstrated that MaNAC4 and MaNAC6, as well as their C-terminal regions, possessed trans-activation activity. Gene expression profiles in fruit with four different ripening characteristics, including natural, ethylene-induced, 1-methylcyclopropene (1-MCP)-delayed, and a combination of 1-MCP with ethylene treatment, revealed that the MaNAC genes were differentially expressed in peel and pulp during post-harvest ripening. MaNAC1 and MaNAC2 were apparently upregulated by ethylene in peel and pulp, consistent with the increase in ethylene production. In contrast, MaNAC3 in peel and pulp and MaNAC5 in peel were constitutively expressed, and transcripts of MaNAC4 in peel and pulp and MaNAC6 in peel decreased, while MaNAC5 or MaNAC6 in pulp increased slightly during fruit ripening. Furthermore, the MaNAC2 promoter was activated after ethylene application, further enhancing the involvement of MaNAC2 in fruit ripening. More importantly, yeast two-hybrid and bimolecular fluorescence complementation analyses confirmed that MaNAC1/2 physically interacted with a downstream component of ethylene signalling, ethylene insensitive 3 (EIN3)-like protein, termed MaEIL5, which was downregulated during ripening. Taken together, these results suggest that MaNACs such as MaNAC1/MaNAC2, may be involved in banana fruit ripening via interaction with ethylene signalling components.
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http://dx.doi.org/10.1093/jxb/ers178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430993PMC
September 2012

Induction of jasmonate signalling regulators MaMYC2s and their physical interactions with MaICE1 in methyl jasmonate-induced chilling tolerance in banana fruit.

Plant Cell Environ 2013 Jan 21;36(1):30-51. Epub 2012 Jun 21.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou, China.

MYC2, a basic helix-loop-helix (bHLH) transcription factor, is a key regulator in the activation of jasmonate (JA) response. However, the molecular details of MYC2 involving in methyl jasmonate (MeJA)-induced chilling tolerance of fruit remain largely unclear. In the present work, two MYC2 genes, MaMYC2a and MaMYC2b, and one homolog of the inducer of the C-repeat-binding factor (CBF) gene, MaICE1 were isolated and characterized from banana fruit. MaMYC2s and MaICE1 were found to be all localized in the nucleus. In addition, the proline-rich domain (PRD) and the acidic domain (AD) in the N-terminus were important for the transcriptional activation of MaMYC2 in yeast cells. Unlike MaICE1's constitutive expression, MaMYC2a and MaMYC2b were induced rapidly following MeJA treatment during cold storage. Moreover, protein-protein interaction analysis confirmed that MaMYC2s interacted with MaICE1. The expression of ICE-CBF cold-responsive pathway genes including MaCBF1, MaCBF2, MaCOR1, MaKIN2, MaRD2 and MaRD5 was also significantly induced by MeJA. Taken together, our work provides strong evidence that MaMYC2 is involved in MeJA-induced chilling tolerance in banana fruit through physically interacting and likely functionally coordinating with MaICE1, revealing a novel mechanism for ICE1 in response to cold stress as well as during development of induced chilling tolerance.
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http://dx.doi.org/10.1111/j.1365-3040.2012.02551.xDOI Listing
January 2013

Expression of genes associated with ethylene-signalling pathway in harvested banana fruit in response to temperature and 1-MCP treatment.

J Sci Food Agric 2011 Mar 23;91(4):650-7. Epub 2010 Dec 23.

Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, China.

Background: Little attention has been paid to characterising the ethylene-signalling pathway genes in relation to abnormal ripening of harvested banana fruit during storage at high temperature. The aim of the present study was to investigate banana fruit abnormal ripening and the expression of ten genes associated with the ethylene-signalling pathway, namely MaACS1, MaACO1, MaERS1-4 and MaEIL1-4, at high temperature. Changes in these parameters of banana fruit at high temperature in response to 1-MCP pretreatment were also investigated.

Results: High temperature accelerated the decline in fruit firmness, increased ethylene production and inhibited degreening in banana fruit, resulting in fruit abnormal ripening. In addition, the expression of MaACS1, MaACO1, MaERS2, MaERS3, MaERS4, MaEIL1, MaEIL3 and MaEIL4 was enhanced in banana fruit stored at high temperature. However, application of 1-MCP prior to high temperature storage delayed fruit abnormal ripening and simultaneously suppressed the expression of MaACS1, MaERS2, MaERS3, MaEIL1, MaEIL3 and MaEIL4.

Conclusion: The findings of this study suggested that the expression of genes associated with the ethylene-signalling pathway might be involved in banana fruit abnormal ripening at high temperature. Application of 1-MCP suppressed the expression of genes associated with the ethylene-signalling pathway, which may be attributed at least partially to 1-MCP delaying fruit abnormal ripening at high temperature.
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http://dx.doi.org/10.1002/jsfa.4226DOI Listing
March 2011

Norepinephrine plays an important role in antinociceptive modulation of hypothalamic paraventricular nucleus in the rat.

Int J Neurosci 2010 Jun;120(6):428-38

Wuxi 101 Hospital of People Liberty Army, Wuxi, Jiangsu, China.

Our previous study has proven that hypothalamic paraventricular nucleus (PVN) plays a role in antinociception. The effects of studied classical neurotransmitter on PVN antinociceptive modulation were investigated in the rat. The results showed: (1) Pain stimulation increased norepinephrine (NE), but not epinephrine, dopamine (DA), 3,4-dihydroxyphenylacetic acid (DA metabolic product), homovanilic acid (DA metabolic product), serotonin (5-HT), 5-hydroxyindoleacetic acid (5-HT metabolic product), acetycholine (Ach), choline (Ach metabolic product), gamma-aminobutyric acid (GABA), and L-glutamate acid concentrations in the PVN perfusion liquid; (2) PVN stimulation with L-glutamate sodium, which excited local neurons only, did not influence the concentrations of the studied classical neurotransmitter and metabolic product in the PVN perfusion liquid; (3) Microinjection of NE, epinephrine, or L-glutamate sodium into the PVN elevated pain threshold, and local administration of GABA decreased pain threshold in a dose-dependent manner, but PVN administration of Ach, DA, or 5-HT did not change pain threshold; (4) Microinjection of phentolamine (alpha-receptor antagonist) or MK801 [NMDA-receptor antagonist] into the PVN reduced pain threshold, and local administration of bicuculline (GABA-receptor antagonist) raised pain threshold, but PVN administration of propranolol (beta-receptor antagonist), atropine (Muscarinic cholinergic receptor antagonist), 6-OH gallamine (Nicotinic cholinergic receptor antagonist), fluperidol (DA-receptor antagonist), or cyproheptadine (5-HT-receptor antagonist) did not alter pain threshold. The data suggested that endogenous NE, not epinephrine, 5-HT, Ach, GABA, and L-glutamate acid played an important role in the PVN antinociceptive modulation.
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http://dx.doi.org/10.3109/00207450802333649DOI Listing
June 2010

Arginine vasopressin induces rat caudate nucleus releasing acetylcholine to participate in pain modulation.

Peptides 2010 Apr 3;31(4):701-5. Epub 2009 Dec 3.

Clinical Medical College of Yangzhou University (Subei People's Hospital), Yangzhou, Jiangsu 225001, China.

A lot of studies have pointed that acetylcholine (Ach), a classic neurotransmitter can regulate pain process in the caudate nucleus (CdN). Our previous report has proven that arginine vasopressin (AVP) effects on pain modulation in the CdN. The communication was designed to investigate the interaction between AVP and Ach in the rat CdN during the pain process. AVP concentration was determined by radioimmunoassay (RIA) and Ach concentration by high performance liquid chromatography (HPLC). The results showed that pain stimulation increased both AVP and Ach concentrations in the CdN perfusion liquid; AVP increased Ach concentration in the CdN perfusion liquid, while AVP receptor antagonist including d(CH(2))(5)Tyr(Me)AVP (V(1) receptor antagonist) and d(CH(2))(5)[D-Ile(2), Ile(4), Ala-NH(2)(9)]AVP (V(2) receptor antagonist) decreased Ach concentration in the CdN perfusion liquid. The data indicated that the analgesic effect of AVP might be involved in the Ach system in the CdN.
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http://dx.doi.org/10.1016/j.peptides.2009.11.027DOI Listing
April 2010

[Dynamic characteristics of phosphorus in purple paddy soil and its environmental Impact].

Huan Jing Ke Xue 2008 Feb;29(2):434-9

College of Resources and Environment, Southwest University, Chongqing 400716, China.

The dynamic characteristics of phosphorus (P) in the surface water and runoff of paddy field with different P fertilizing treatments were investigated using the field experiment under the independent irrigation system as well as its environmental impact. The results showed that the concentration of total phosphorus (TP) in the surface water increased as the fertilizing amounts enhanced and reached the peak values after 24 h for all treatments in range of 0.928-3.824 mg/L. And the fluctuation of TP concentration in surface water was drastic during the first 30 days with the average contents of 0.259-1.433 mg/L which exceeded the critic values of eutrophication. Therefore, the field managements such as inter-tillage and drainage should be avoided during the time. After 40 days, the TP concentration declined slowly and then came to stabilization with low values after 60 days. The contents of different P forms in the runoff water increased with the improved amounts of precipitation and fertilizing and above 50% was dissolved phosphorus (DP). The DP was the dominant one in the P loss of the purple paddy soil and the loss load changed between 0.358 and 2.579 kg/hm2. Additionally, the P loss more easily occurred for the treatment of utilizing the cattle manure than that of straw, approximately 40% of loss load higher. Both the loss load and apparent P loss ratio evidently declined with the treatment of fertilizer combined with straw, suggesting that it was the better measure for reducing the P loss in the paddy field.
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February 2008

Changes in alpha-L-arabinofuranosidase activity in peel and pulp of banana (Musa sp.) fruits during ripening and softening.

Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao 2007 Apr;33(2):131-6

College of Resources Science and Papermaking Engineering, South China University of Technology, Guangzhou 510640, China.

Arabinose is one of the most dynamic cell wall glycosyl residues released during fruit ripening, alpha-L-arabinofuranosidase (alpha-Arab) are major glycosidases that may remove arabinose units from fruit cell wall polysaccharides. To find out whether alpha-Arab plays important roles in banana fruit softening, the enzyme activities in peel and pulp, fruit firmness, respiration rate and ethylene release rate were assayed during banana softening. The results showed that alpha-Arab activities in banana pulp and peel increased slightly at the beginning of storage and reached their maxima when the fruit firmness decreased drastically, alpha-Arab activity increased by more than ten folds in both pulp and peel during ripening and alpha-Arab activities were higher in pulp than in peel. Treatment of banana fruits with ethylene absorbent postponed the time of reaching of its maxima of respiration and ethylene, enhanced the firmness of pup and decreased alpha-Arab activity in the peel and pulp. These results suggest that alpha-Arab induced the decrease of fruit firmness and played an important role in banana fruit softening, and its activity was regulated by ethylene.
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April 2007

Cloning and expression analysis of beta-galactosidase gene related to softening of banana (Musa sp.) fruit.

Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao 2006 Aug;32(4):411-9

Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.

Beta-galactosidase is thought to be involved in fruit softening through cleaving beta(1-->4) galactan bonds in cell wall hemicellulose. To study the relationship between fruit softening and beta-Gal during banana (Musa sp.) fruit ripening, a beta-Gal cDNA fragment, named MA-Gal, has been cloned from banana fruit pulp using RT-PCR in this study. The results of sequence analysis showed that MA-Gal contained 927 bp, encoding a polypeptide of 309 amino acids, the deduced protein was highly homologous to plant beta-galactosidase expressed in fruit ripening. The MA-Gal putative amino acids have five homologous domains (typical eukaryotic beta-Gals have seven domains), contain the GGPIILSQIENEY(F) motif, which is a presumptive catalytic active site conserved in all beta-Gals. Phylogenetic analysis showed that MA-Gal was in plant beta-Gal group I, which was expressed in fruit tissue. The changes in beta-Gal activity and firmness in pulp during three different stages were also assayed. The Northern analysis showed that the MA-Gal transcripts in pulp were detected at low level at preclimacteric stage, while an increasing progression was observed during fruit ripening, and the highest transcript amount was found at the postclimacteric stage. These results suggest that MA-Gal may play a role during banana fruit ripening and softening.
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August 2006

[The dynamic changes of heme oxygenase-1 mRNA and protein express at subfornical organ in rats with experimental allergic encephalomyelitis].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2006 Feb;22(1):109-12

Department of Neurology, 2nd Hospital, Hebei Medical University, Shijiazhuang 050000, China.

Aim: To observe the dynamic changes of heme oxygenase-1 (HO-1) mRNA and protein express in subfornical organ in rats with experimental allergic encephalomyelitis (EAE) to confirm that SFO is one of the sites for blood-bearing signaling molecules entering into brain.

Methods: EAE was induced by CFA-GPSCH on Wistar rats, we observed the levels of HO-1 mRNA and its protein expression with immunohistochemistry and in situ hybridization technology on 1 d, 7 d, 14 d, and 21 d after EAE induction in SFO of rats. The relationship between HO-1 and symptoms of EAE was also investigated.

Results: The expression levels of HO-1 mRNA and its protein expression were very low in the brains of the control group, whereas they were enhanced gradually with pathological course in the brain and onsets of symptoms, signs of EAE. On 1 d after induction of EAE, positive cells of HO-1 mRNA and its protein expression were observed at SFO, but the labeled cells were rarely seen in the other brain regions. On 7 d, the positive cells increased markedly. On 14 d the levels of HO-1 mRNA and its protein expression in the brains reached the peak, the positive cells of HO-1 were mainly located at the choroid plexuses and SFO, as well as the regions around "sleeve-like" lesion foci, all of which were coincident with the locations of lesions of EAE. The changes of incidence, symptom, reduction of the body weight, and pathology lesions of EAE in rat brains were the most significant. On 21 d, the levels of HO-1 mRNA and its protein expression reduced gradually, which was in parallel with remitted symptoms of EAE. When a specific inhibitor of HO-1, Snpp9, was applied, the symptoms and pathological lesions of EAE in brains were mitigated markedly.

Conclusion: SFO may be one of the earliest sites for blood-bearing signaling molecules entering into brain. The dynamic changes of HO-1 mRNA and its protein expression are in parallel with the changes of symptoms and pathological lesions of EAE in the brains. Application of some inhibitors of HO-1 may be one of potential therapeutic methods for prevention and treatment of EAE.
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February 2006

Soybean DRE-binding transcription factors that are responsive to abiotic stresses.

Theor Appl Genet 2005 May 20;110(8):1355-62. Epub 2005 Apr 20.

National Key Laboratory of Plant Genomic, Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing, 100101, China.

Three DREB homologue genes, GmDREBa, GmDREBb, and GmDREBc, were isolated from soybean, Glycine max (L.) Merr. Each of the deduced proteins contains an AP2 domain of 64 amino acids. Yeast one-hybrid assay revealed that all of the three dehydration-responsive, element-binding proteins specifically bound to the dehydration-responsive element. Analysis of transcriptional activation abilities of these proteins in yeast indicated that GmDREBa and GmDREBb could activate the expression of a reporter gene, whereas GmDREBc could not. The transcriptions of GmDREBa and GmDREBb were induced by salt, drought, and cold stresses in leaves of soybean seedlings. The expression of GmDREBc was not significantly affected in leaves but apparently induced in roots by salt, drought, and abscisic acid treatments. These results suggest that these three genes function specifically in response to abiotic stresses in soybean.
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http://dx.doi.org/10.1007/s00122-004-1867-6DOI Listing
May 2005

Intracerebroventricular administration of adrenomedullin increases the expression of c-fos and activates nitric oxide-producing neurons in rat cardiovascular related brain nuclei.

Sheng Li Xue Bao 2004 Jun;56(3):328-34

Department of Physiology, Hebei Medical University, Shijazhuang 050017, China.

To define the action sites of adrenomedullin (ADM) in the rat brain, and to examine whether neuronal NO may participate in the actions of ADM, the present study was undertaken to examine the effects of i.c.v. administration of ADM on the induction of Fos protein and on nitric oxide-producing neurons in rat brain nuclei involved in cardiovascular regulation, using double immunohistochemical method for Fos and neuronal nitric oxide synthase (nNOS). Following i.c.v. administration of ADM (1 nmol/kg, 3 nmol/kg), Fos-like immunoreactivity neurons were markedly increased in several brain areas of the rat, including the nucleus of the solitary tract (NTS), the area postrema, the locus coeruleus, the parabrachial nucleus and the nucleus paragigantocelluaris laterialis (PGL) in the brainstem, the paraventricular nucleus (PVN), the supraoptic nucleus (SON) and the ventromedial hypothalamic nucleus in the hypothalamus, as well as the central amygdaloid nucleus and the lateral habenular nucleus in the forebrain. Following i.c.v. injection of ADM (1 nmol/kg, 3 nmol/kg), the number of double-labeled neurons for Fos and nNOS was increased in the PVN and SON. Small numbers of double-labeled neurons were also found in the NTS and PGL following i.c.v. injection of ADM (3 nmol/kg), while i.c.v. injection of ADM (1 nmol/kg) did not change the number of double-labeled neurons in the NTS and PGL. Pretreatment with calcitonin gene-related peptide receptor antagonist CGRP(8-37) (30 nmol/kg) significantly reduced the action of ADM (3 nmol/kg) in the brain. These results suggest that centrally administered ADM may increase the expression of c-fos in the forebrain, the hypothalamus and the brainstem and activate nitric oxide-producing neurons in the PVN, SON, NTS and PGL. These effects may be partly mediated by CGRP receptors.
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June 2004
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