Publications by authors named "Xue-Mei Tian"

21 Publications

  • Page 1 of 1

Specific oral and maxillofacial identifiers in panoramic radiographs used for human identification.

J Forensic Sci 2021 Jan 27. Epub 2021 Jan 27.

Institute of Forensic Science, Ministry of Public Security, Beijing, China.

Radiographically assisted dental identification is an important means for individual identification. Specific identifiers help to quickly filter some of the possible corresponding AM and PM images at the beginning. The study seeks specific oral and maxillofacial identifiers in panoramic radiographs. A total of 920 panoramic radiographs from 460 live patients were used. The most recent radiograph served as the surrogate post-mortem (PM) record of an unidentified person, and the earliest radiograph served as the ante-mortem (AM) record of the same person. We evaluated the following four groups of identifiers of the images: (1) dental morphology, tooth number, and position; (2) dental treatment and pathology; (3) morphological identifiers of the jaw; and (4) pathological identifiers of the jaw. The ratio of each identifier being identified simultaneously in the AM and PM databases was determined. Specific identifiers were defined as those that appeared at low frequency (ratio: 0%-0.250%). A total of 18 specific oral and maxillofacial identifiers were determined. The specific identifiers were a retained deciduous tooth (0.011%), S-shaped deflection of a tooth root (0.012%), distal deflection of tooth root (0.017%), inverted impaction (0.018%), malposition (0.038%), supernumerary teeth (0.061%), mesial deflection of tooth root (0.092%), microdontia (0.136%), buccal/lingual impaction (0.188%), cementoma (0.002%), hypercementosis (0.002%), continuous crown (0.004%), pulp calcification (0.023%), attrition (0.030%), residual root (0.106%), root resorption (0.137%), implant (0.156%), and osteomyelitis (0.002%). Identifiers of the teeth and jaw can be used for human identification, and dental identifiers are more specific than identifiers of jaw.
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http://dx.doi.org/10.1111/1556-4029.14673DOI Listing
January 2021

Adipocyte-Derived Leptin Promotes PAI-1 -Mediated Breast Cancer Metastasis in a STAT3/miR-34a Dependent Manner.

Cancers (Basel) 2020 Dec 21;12(12). Epub 2020 Dec 21.

New Drug Screening Center, China Pharmaceutical University, Nanjing 210009, China.

The crosstalk between cancer cells and adipocytes is critical for breast cancer progression. However, the molecular mechanisms underlying these interactions have not been fully characterized. In the present study, plasminogen activator inhibitor-1 (PAI-1) was found to be a critical effector of the metastatic behavior of breast cancer cells upon adipocyte coculture. Loss-of-function studies indicated that silencing PAI-1 suppressed cancer cell migration. Furthermore, we found that PAI-1 was closely related to the epithelial-mesenchymal transition (EMT) process in breast cancer patients. A loss-of-function study and a mammary orthotopic implantation metastasis model showed that PAI-1 promoted breast cancer metastasis by affecting the EMT process. In addition, we revealed that leptin/OBR mediated the regulation of PAI-1 through the interactions between adipocytes and breast cancer cells. Mechanistically, we elucidated that leptin/OBR further activated STAT3 to promote PAI-1 expression via miR-34a-dependent and miR-34a-independent mechanisms in breast cancer cells. In conclusion, our study suggests that targeting PAI-1 and interfering with its upstream regulators may benefit breast cancer patients.
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http://dx.doi.org/10.3390/cancers12123864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767398PMC
December 2020

Three new species of (Polyporales, Basidiomycota) from China.

MycoKeys 2020 14;72:1-16. Epub 2020 Aug 14.

Shandong Provincial Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao 266109, China Qingdao Agricultural University Qingdao China.

In this study, taxonomic and phylogenetic analyses of were performed. Three new species were characterised according to morphological characteristics and molecular phylogenetic analysis using ITS and nLSU sequences. They are , and is characterised by resupinate, thin basidiomata with white to buff-yellow hymenophore, small pores (9-11 per mm), clamped generative hyphae possessing hymenial cystidia, ellipsoid basidiospores (2.5-3 × 1.7-2 µm) and growth on fallen bamboo or angiosperm branch. is characterised by resupinate basidiomata with pink to salmon pores and a distinct white margin, clamp generative hyphae, interwoven tramal hyphae, ellipsoid basidiospores measuring 2.6-3.2 × 1.8-2 µm and growth on . is characterised by resupinate basidiomata with pale salmon to brownish vinaceous hymenophore, small pores (10-12 per mm), generative hyphae with simple septa and clamp connections, interwoven tramal hyphae, lunate basidiospores measuring 2.9-3.4 × 1.6-1.8 µm and thriving on rotten wood of angiosperms.
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http://dx.doi.org/10.3897/mycokeys.72.51872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442768PMC
August 2020

Taxonomy and phylogeny of (Hymenochaetales, Basidiomycota): four new species and keys to species of the genus.

MycoKeys 2020 7;68:115-135. Epub 2020 Jul 7.

Shandong Provincial Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao 266109, China Qingdao Agricultural University Qingdao China.

is a polypore genus with white to cream or buff basidiomata, whose species in Hymenochaetales are poorly known. We study the phylogeny and diversity of based on our recent collections from tropic and subtropic Asian-Pacific regions. Phylogenetic analyses based on the internal transcribed spacer (ITS) and nuclear large subunit (nLSU) ribosomal RNA gene regions indicate that ten terminal lineages are well supported within . Based on morphological examination and phylogeny, four new species, viz. , , and are described, and a new combination, , is proposed. All these species are illustrated. is characterized by tiny basidiomata with bluish pores when fresh, generative hyphae dominating at the dissepiment edges, the presence of cystidioles, and allantoid basidiospores measuring 3.8-4.4 × 0.9-1.3 μm. differs from other poroid species in the genus by having parallel tramal hyphae in combination with lunate basidiospores measuring 2.8-3.3 × 0.9-1.2 μm. have generative and skeletal hyphae co-dominating at the dissepiment edges, and lunate basidiospores measuring 3.5-4 × 1-1.3 μm. is distinguished by small pores (8-10 per mm) and relatively long allantoid basidiospores measuring 4.2-5 × 0.8-1 μm. is characterized by buff to olivaceous buff basidiomata when dry, 5-7 pores per mm, rosette-like crystals rare, and allantoid basidiospores measuring 3.7-4.3 × 1-1.3 μm. An identification key to all accepted species is provided.
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http://dx.doi.org/10.3897/mycokeys.68.53561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360635PMC
July 2020

Turn-On Luminescent Sensor toward Fe, Cr, and Al Based on a Co(II) Metal-Organic Framework with Open Functional Sites.

Inorg Chem 2020 Mar 19;59(5):2803-2810. Epub 2020 Feb 19.

School of Chemistry and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, Jiangxi Province, P.R. China.

A novel Co-based metal-organic framework (MOF) with the formula of {[Co(BIBT)(BTC)(HO)]·solvents} (, where denotes Jiangxi University of Science and Technology, BIBT = 4,7-bi(1-imidazol-1-yl)benzo-[2,1,3]thiadiazole, and HBTC = 1,3,5-benzenetricarboxylic acid) has been solvothermally prepared, which takes 3D structure with a rare 3,4,6-c topology and contains intramolecular hydrogen bonds. Interestingly, the sensing investigations suggest that could be considered as a multifunctional fluorescence sensor toward Fe, Cr, and Al via a turn-on effect with good reusability and detection limits of 0.13, 0.10, and 0.10 μM, respectively. The turn-on effect of could be ascribed to an absorbance caused enhancement (ACE) mechanism. Notably, is the first turn-on MOF fluorescent sensor for Fe, Cr, and Al simultaneously.
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http://dx.doi.org/10.1021/acs.inorgchem.9b03152DOI Listing
March 2020

A Cd -Based Metal-Organic Framework with pcu Topology as Turn-On Fluorescent Sensor for Al.

Chem Asian J 2019 Oct 24;14(20):3648-3654. Epub 2019 Jul 24.

School of Materials Science and Engineering, National Institute for Advanced Materials, Nankai University, Tianjin, 300350, P. R. China.

A new metal-organic framework (MOF) {[Cd (bbib) (ndc) ]⋅2DMF} (JXUST-1) (bbib=1,3-bis(benzimidazolyl)benzene, H ndc=1,4-naphthalenedicarboxylic acid, DMF=N,N-dimethylformamide) has been solvothermally synthesized and characterized by single-crystal X-ray diffraction, PXRD, TGA, IR and elemental analysis. JXUST-1 exhibits a three-dimensional 6-connected pcu topology with a Schläfli symbol {4 .6 } constructed by [Cd (CO ) ] secondary building units. Fluorescence studies show that this MOF can sensitively and selectively recognize Al via a fluorescence enhancement effect, and the detection limit is 0.048 ppm. Furthermore, JXUST-1 displays relatively good thermal and chemical stabilities as well as reusability. All these results suggest JXUST-1 to be a highly selective and recyclable luminescent sensing material for the detection of Al .
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http://dx.doi.org/10.1002/asia.201900739DOI Listing
October 2019

Phylogeny of the genus and taxonomic assessment of the group.

Mycologia 2019 May-Jun;111(3):423-444. Epub 2019 Apr 9.

a Beijing Advanced Innovation Center for Tree Breeding by Molecular Design , Beijing Forestry University , Beijing 100083 , China.

The genus of the Hymenochaetaceae is characterized by resupinate to pileate basidiocarps, a dimitic hyphal system with fine crystal aggregates and encrusted generative hyphae in dissepiment edge and tube trama, the presence of hymenial setae, and hyaline, thin-walled, smooth basidiospores. Members of the group are easy to distinguish from other species of because of the moderately large pores, presence of mycelial setae, and large hymenial setae. Here, we explore phylogenetic relationships among 20 species of based on examination of some 90 collections sampled worldwide. Seven new species are recognized in the group-, and -described from China, Costa Rica, Mexico, and the United States. Phylogenetic analyses inferred from DNA sequences of the nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS2 = ITS), D1-D2 domains of nuc 28S rDNA (28S), and translation elongation factor EF-1 alpha () support the group as one of two major clades within comprising nine species worldwide.
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http://dx.doi.org/10.1080/00275514.2019.1570749DOI Listing
April 2020

A Zn-Based Metal-Organic Framework with a Rare tcj Topology as a Turn-On Fluorescent Sensor for Acetylacetone.

Inorg Chem 2019 Mar 1;58(6):3578-3581. Epub 2019 Mar 1.

School of Metallurgy and Chemical Engineering , Jiangxi University of Science and Technology , Ganzhou 341000 , Jiangxi Province , P. R. China.

A Zn-based metal-organic framework (MOF) with a rare tcj topology has been solvothermally synthesized and displays relatively good thermal and chemical stabilities. Interestingly, the MOF can sensitively and selectively sense acetylacetone (acac) via a fluorescence enhancement effect with a detection limit of 0.10 ppm and good reusability, which demonstrates the first example of a MOF-based turn-on fluorescent sensor for acac.
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http://dx.doi.org/10.1021/acs.inorgchem.8b03316DOI Listing
March 2019

Three new species of (Hymenochaetales, Basidiomycota) based on the evidence from morphology and DNA sequence data.

MycoKeys 2018 8(30):73-89. Epub 2018 Mar 8.

Beijing advanced innovation center for tree breeding by molecular design, Beijing Forestry University, Beijing 100083, PR China.

, and are described and illustrated as new species based on morphological characters and molecular evidence. They have annual to perennial, mostly resupinate basidiomata with grayish fresh pores, an indistinct subiculum, lack any kind of setae, have brownish, thick-walled basidiospores, and cause a white rot. The distinctive morphological characters of the new species and their related species are discussed. Phylogenies based on the nuclear ribosomal large subunit (28S) and the nuclear ribosomal ITS region show that these three new species form three distinct lineages in the clade. A key to known species of is given.
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http://dx.doi.org/10.3897/mycokeys.30.23109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904509PMC
March 2018

Global diversity and phylogeny of Onnia (Hymenochaetaceae) species on gymnosperms.

Mycologia 2017 4;109(1):27-34. Epub 2017 Jan 4.

a Institute of Microbiology, PO Box 61, Beijing Forestry University , Beijing , China.

Onnia includes white rotting polypores with annual basidiocarps, a duplex context, monomitic hyphal structure, hymenial setae, and hyaline, thin-walled, smooth basidiospores. Specimens of Onnia, originating mainly from East Asia, Europe, and North America, were studied using both morphology and phylogenetic analyses. Our concatenated data set was derived from 25 collections and included (i) 25 nuc rDNA internal transcribed spacer region sequences (ITS1-5.8S-ITS2 = ITS), 17 generated in this study; and (ii) 14 nuc rDNA 28S rDNA sequences, including the D1-D2 domains, 11 of them generated in this study. The resulting maximum likelihood and Bayesian phylogenies recovered all sampled collections of Onnia as a well-supported clade. In this clade, three previously accepted species, viz., Onnia leporina, O. tomentosa, and O. triquetra, received strong support, whereas three additional lineages with strong support represent the new species described in this paper, O. subtriquetra, O. microspora, and O. tibetica. Of the six Onnia species occurring on gymnosperms, O. tomentosa and O. leporina grow mainly on Picea and have circumboreal distribution in the Northern Hemisphere. In contrast, other species that mostly grow on Pinus are geographically restricted to limited regions, viz., O. triquetra in Europe, O. subtriquetra in North America, and O. microspora and O. tibetica in Asia.
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http://dx.doi.org/10.1080/00275514.2016.1274619DOI Listing
May 2018

High-Level Production of Exopolysaccharides by a Cosmic Radiation-Induced Mutant M270 of the Maitake Medicinal Mushroom, Grifola frondosa (Agaricomycetes).

Int J Med Mushrooms 2016 ;18(7):621-30

College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, China.

A new Grifola frondosa mutant, M270, was successfully isolated for high production of exopolysaccharides (EPSs) using cosmic radiation-induced mutagenesis. We found that the mutant M270 had a clearer and thicker EPS layer (~10 µm) adhering to mycelia than those of its parent strain 265 after Congo red staining. In the 20-L batch fermentation for M270, 10.3 g/L of EPS and 17.9 g/L of dry mycelia biomass were obtained after 204 hours of fermentation. Furthermore, a main water-soluble fraction (EP1) in the EPS was purified from M270 and then confirmed to be heteroglycan-protein complex with 91% (w/w) total carbohydrates and 9% (w/w) total proteins. Four kinds of monosaccharide-D-mannose, D-glucosamine, D-glucose, and D-xylose-were detected in EP1 with a molar ratio of 17.6:1.8:100:2.5. The molecular mass of the main component in EP1 was 8.9 kDa. The EPS from M270 significantly inhibited the growth of sarcoma 180 solid tumors in mice. This G. frondosa M270 mutant could serve as a better candidate strain for polysaccharide production.
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http://dx.doi.org/10.1615/intjmedmushrooms.v18.i7.70DOI Listing
March 2017

Optimization of Liquid Fermentation Medium for Production of Inonotus sanghuang (Higher Basidiomycetes) Mycelia and Evaluation of their Mycochemical Contents and Antioxidant Activities.

Int J Med Mushrooms 2015 ;17(7):681-91

Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.

Inonotus sanghuang, an authentic "Sanghuang" mushroom used in traditional Chinese medicine, is known to possess important pharmacological activities. In this study, we aimed to optimize the liquid fermentation medium for I. sanghuang mycelial production and to determine the effects of two-stage cultivation (shake and static) on the yield of total flavonoids, total phenolics, and polysaccharides, as well as the antioxidant activities of I. sanghuang mycelial extracts (ISME). Under an optimized medium composition (38.96 g/L of corn flour, 4.15 g/L of yeast extract, 20.55 g/L of bran and pH 6.39), the predicted and experimental optimal mycelial biomasses were 17.60 g/L and 18.33±0.86 g/L, respectively. The results of two-stage cultivation showed that contents of total flavonoids and total phenolics in mycelia increased by 37.92% and 77.27%, respectively. However, irregular polysaccharide contents were noted throughout the experimental period. Antioxidant assays showed that ISME possessed good free-radical scavenging activity, which is mainly contributed by polyphenolic-type metabolites.
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http://dx.doi.org/10.1615/intjmedmushrooms.v17.i7.80DOI Listing
August 2016

[Physiological responses of cucumber seedlings grafted on different salt-tolerant rootstocks to NaCI stress].

Ying Yong Sheng Tai Xue Bao 2012 Jan;23(1):147-53

College of Horticultural Science and Engineering, Shandong Agricultural University, Tai' an 271018, Shandong, China.

Based on the salt-tolerance identification of a series of cucumber rootstock varieties, the cucumber cultivar 'Xintaimici' grafted on different salt-tolerant rootstock varieties 'Sherpa', 'Shintosa' , 'Tielizhen' , and 'Figleaf gourd' was selected to study its seedlings physiological responses to NaCl stress, taking the self-rooted ones as the control. Under the stress of 100 mmol NaCl x L(-1), the leaf electrolyte leakage rate and malondialdehyde (MDA) content of the grafted seedlings were significantly lower than those of self-rooted seedlings, and the seedlings grafted on 'Figleaf gourd' showed the lowest electrolyte leakage rate and malondialdehyde (MDA) content, followed by the seedlings grafted on 'Tielizhen', 'Shintosa' , and 'Sherpa'. The leaf proline and soluble sugar contents and peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activities of the grafted seedlings were significantly higher than those of the self-rooted seedlings, and the POD, SOD, CAT, and APX activities were the highest for the seedlings grafted on 'Figleaf gourd' and the lowest for the seedlings grafted on 'Sherpa', but had no significant differences for the seedlings grafted on 'Tielizhen' and 'Shintosa'. The leaf Na+ content of the seedlings grafted on different rootstock varieties ranked as 'Figleaf gourd' < 'Tielizhen' < 'Shintosa' < 'Sherpa', while the leaf K+ content had little difference for the seedlings grafted on 'Figleaf gourd', 'Tielizhen', and 'Shintosa' but was significantly higher than that for the seedlings grafted on 'Sherpa'. The self-rooted seedlings had the highest leaf Na+ content but the lowest leaf K+ content. The leaf Na+/K+ ratio of grafted seedlings was significantly lower than that of self-rooted ones, and the seedlings grafted on 'Figleaf gourd' had the lowest leaf Na+/K+ ratio.
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January 2012

[Comparative study of primordial germ cells in male and female mouse embryos].

Nan Fang Yi Ke Da Xue Xue Bao 2010 Sep;30(9):2175-8

Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.

Objective: To investigate the differences in the development of primordial germ cells (PGCs) between male and female mouse embryos.

Methods: The morphological changes of genital ridge development were detected in C57BL/6J mouse embryos of 11-13.5 days, and the changes of PGCs quantity and proliferation were compared between the male and female embryos using immunofluorescence histochemistry.

Results: The PGCs was the most numerous at 13.5 days in male and female embryos, and the quantity of proliferating PGCs reached the maximum at 13 days. The quantity of PGCs and proliferating PGCs in male embryos at 13 days was significantly larger than that in female embryos.

Conclusion: The development of PGCs is characterized by a gender differences in early development of mouse embryos (11-13.5 days).
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September 2010

[Flow cytometric analysis for detecting mitochondrial permeability transition pore opening].

Nan Fang Yi Ke Da Xue Xue Bao 2010 Sep;30(9):2097-9

Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.

Objective: To introduce a new method for detecting mitochondrial permeability transition pore (PTP) opening with flow cytometry using the resveratrol-inducing PTP opening model.

Methods: Mitochondria were isolated from rat livers and selectively labeled with nonyl acridine orange. The mitochondrial membrane potential was detected using flow cytometry with TMRE (tetramethylrhodamine, ethyl ester) labeling. PTP opening induced by resveratrol was represented by the changes of mitochondrial side-scattering (SSC) detected by flow cytometry.

Results: Flow cytometry was capable of defining the purity of the mitochondria isolated. The fluorescence intensities and SSC of the mitochondria were decreased after resveratrol treatment, indicating that resveratrol could induce PTP opening. Ciclosporin A inhibited resveratrol-induced PTP opening.

Conclusion: Flow cytometric analysis allows accurate and convenient detection of mitochondrial membrane potential, mitochondrial swelling and PTP opening.
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September 2010

Exogenous Nanog alleviates but is insufficient to reverse embryonic stem cells differentiation induced by PI3K signaling inhibition.

J Cell Biochem 2009 Apr;106(6):1041-7

State Key Laboratory of Experimental Hematology, Institute of Hematology, National Research Center for Stem Cell Engineering and Technology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.

PI3K signaling pathway plays a significant role in embryonic stem cells (ES cells) self-renewal. Overexpression of Nanog maintains mouse ES cells pluripotency independent of leukemia inhibitory factor (LIF). However, little is known about the effect of PI3K signaling pathway on ES cells with Nanog overexpression. Our experiments aimed to explore the relationship between PI3K signaling pathway and Nanog expression in ES cells. We observed the effect of LY294002, a specific inhibitor of PI3K pathway, on wild-type J1 cells and Nanog overexpressing (Ex-Nanog) J1 cells in the presence or absence of LIF. With LY294002 treatment, both of them lost their ES features even in the presence of LIF. But the differentiation induced by LY294002 on Ex-Nanog J1 cells was slighter lower than that on wild-type J1 cells. These results indicate that inhibition of PI3K pathway induces mouse ES cells differentiation. Exogenous Nanog sustains mouse ES cells pluripotency independent of LIF, and alleviates the differentiation induced by LY294002. But it is insufficient to totally reverse the differentiation.
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http://dx.doi.org/10.1002/jcb.22082DOI Listing
April 2009

[Effects of resveratrol on growth inhibition and gap-junctional intercellular communication of HepG2 cells].

Nan Fang Yi Ke Da Xue Xue Bao 2006 Jul;26(7):963-6

Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.

Objective: To investigate the effects of the resveratrol on proliferation and gap-junctional intercellular communication (GJIC) in human liver cancer cell line HepG2.

Methods: MTT assay was used to observe the effects of resveratrol on HepG2 cell growth, and the distribution of cell cycles was detected with flow cytometry (FCM). The effects of resveratrol on GJIC of HepG2 cells labeled with 5'-CFDA/AM was examined with fluorescence redistribution after photobleaching (FRAP) and confocal microscope.

Results: The results of MTT assay indicated that the proliferation of HepG2 cells was significantly inhibited by resveratrol in a time- and dose-dependent manner. Resveratrol could arrest HepG2 cell growth in S phase, inhibit DNA synthesis and induce cell apoptosis. Furthermore, the levels of GJIC increased sharply after resveratrol treatment of the cells.

Conclusion: Resveratrol is capable of inhibiting HepG2 cell proliferation, causing cell growth arrest at S phase and inducing cell apoptosis. Increased GJIC level contributes to the effect of resveratrol in HepG2 cell proliferation inhibition and its cancer chemopreventive activity.
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July 2006

[Resveratrol promotes Ca2+-induced Ca2+ release from rat liver cell mitochondria mediated by Ca2+].

Nan Fang Yi Ke Da Xue Xue Bao 2006 Jul;26(7):910-3

Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.

Objective: To investigate the effects of resveratrol (Res) on mitochondrial opening and Ca(2+)-induced Ca(2+) release (CICR) from rat liver cell mitochondria mediated by Ca(2+).

Methods: Wistar rat liver cell mitochondria was extracted and Res-induced mitochondrial swelling was assessed spectrophotometrically at 540 nm to examine the permeability transition pore (PTP) opening. The membrane potential changes of Res-treated mitochondria were measured with fluorescence spectrophotometery. Ca(2+) uptake and release by the mitochondria was determined by absorbance change of arsenazo III at 685-675 nm monitored by dual wavelength spectrophotometry.

Results: Res promoted Ca(2+)-mediated PTP opening, and this effect was completely inhibited by CsA and lowered by trifluoperazine. CICR accelerated by Res treatment was completely blocked by ruthenium red and partly by trifluoperazine.

Conclusion: Res can promote PTP opening by inducing CICR, which may be one of the pathways that Res induces cell apoptosis.
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July 2006

[Mechanism of opening of mitochondrial permeability transition pore induced by arsenic trioxide].

Ai Zheng 2006 Jan;25(1):17-21

Instrumental Analysis & Research Center, Southern Medical University, Guangzhou, Guangdong 510515, P. R. China.

Background & Objective: Permeability transition pore (PTP) is central for apoptosis by acting as a good candidate pathway for the release of cytochrome c and apoptosis-induction factors from mitochondria. Arsenite may induce apoptosis via a direct effect on PTP. To characterize the exact mechanism for arsenite to induce PTP opening, the correlations of calcium-induced calcium release from mitochondria (mCICR) to As2O3-induced PTP opening and cytochrome c release from mitochondria were studied.

Methods: Mitochondria were prepared from Wistar rat livers. The effect of As2O3 on mitochondrial PTP opening was measured with ultraviolet (UV) spectrophotometer. The changes of Ca(2+) concentration were detected with UV spectrophotometer to monitor Ca(2+) -induced Ca(2+) release from mitochondria. Cytochrome c release mediated by Ca(2+) was measured with Western blot.

Results: As2O3 (10 micromol/L) combined with low concentration of Ca(2+) didn't induce PTP opening and cytochrome c release from mitochondria; while As2O3 (10 micromol/L) combined with high concentration of Ca(2+) induced PTP opening and cytochrome c release. When mCICR was inhibited, the effect of As2O3 and Ca(2+) on PTP opening and cytochrome c release was completely inhibited.

Conclusion: As2O3-induced PTP opening and cytochrome c release depend on mCICR.
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January 2006

[An investigation of the relationship between heavy drinking and alcoholic fatty liver in the Xinjiang minority ethnic group].

Zhonghua Gan Zang Bing Za Zhi 2005 Nov;13(11):849-51

Department of Infectious Diseases, Friend Hospital of Yili, Yili 835000, China.

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November 2005

[Resveratrol promote permeability transition pore opening mediated by Ca2+].

Yao Xue Xue Bao 2003 Feb;38(2):81-4

Department of Histology and Embryology, First Millitary Medical University, Guangzhou 510515, China.

Aim: To investigate the mechanisms of anti-cancer effect of resveratrol (Res), and the effects of Res in cell apoptosis. The role of Res playing in mitochondrial permeability transition pore (PTP) induction was studied.

Methods: Mitochondria was prepared from the liver of Wistar rats. The effects of Res on oxygen consumption of isolated mitochondria from rat liver was measured with Clark-type electrode and resulted in respiration control rate (RCR). Mitochondrial swelling affected by Res was assessed spectrophotometrically, through the changes in absorbance at 540 nm. The PTP opening was learned from the results. Membrane potential of mitochondia was measured through fluorescence spectrophotometry.

Results: Res was shown to inhibit the respiration and decrease the RCR of mitochondria. Res can promote the PTP opening mediated by Ca2+. Res was shown to promote the increase of mitochondial membrane potential mediated by Ca2+ and loss of mitochondial membrane potential.

Conclusion: Res was shown to inhibit mitochondial respiration and induce PTP opening of mitochondria. These may be one of the pathways that Res showed anti-cancer action and induce cells apoptosis.
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February 2003