Publications by authors named "Xue Fu"

288 Publications

Removal of emulsified oil from water by using recyclable chitosan based covalently bonded composite magnetic flocculant: Performance and mechanism.

J Hazard Mater 2021 Jun 30;419:126529. Epub 2021 Jun 30.

Engineering Research Center of Biofilm Water Purification and Utilization Technology, Ministry of Education, Anhui University of Technology, Maanshan, Anhui 243002, China.

In this work, a novel recyclable covalently bonded magnetic flocculant (FS-MC) was successfully prepared by combining chitosan-based modified polymers (MCS) with [email protected] through a silane coupling agent. The covalent bond Fe-O-Si-O-C and the core-shell structure of FS-MC were confirmed through several characterization methods. The emulsified oily wastewater flocculation performance and mechanism by using FS-MC were evaluated and studied. Results showed that 94.47%, 93.95%, and 92.98% of emulsified oil could be removed by using FS-MC1, FS-MC2 and FS-MC3 at dosages of 2.0, 2.5, and 2.0 mg/L, respectively. Furthermore, FS-MC exhibited an excellent behavior on the removal of organic compounds with molecular weight > 10 kDa, including long chain alkanes, cycloalkanes, and aromatic hydrocarbon compounds. In addition, triple-phase separation of oil, water and flocculants was achieved by using magnetic FS-MC. Due to the introduction of cationic and hydrophobic groups in FS-MC, charge neutralization, compression double electric-layer action, hydrophobic interaction, interfacial adsorption bridging and sweep-flocculation synergistically contributed and enhanced the removal of emulsified oil. Recycling experiments also showed that no obvious decrease of oil removal rate was observed by using magnetic FS-MC flocculants in five cycles.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126529DOI Listing
June 2021

Assessing association between mean systemic filling pressure and acute kidney injury after cardiac surgery.

Acta Anaesthesiol Scand 2021 Jul 26. Epub 2021 Jul 26.

Department of Anaesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

With a great interest we read the recent article by Neuman et al assessing the association between mean systemic filling pressure analogue (P ) and acute kidney injury (AKI) following cardiac surgery. They showed that Pmsa as a surrogate for renal venous pressure was more closely associated with AKI than central venous pressure. As AKI has been significantly associated with morbidity and mortality in after cardiac surgery, their findings have potential implication. Other than the limitations described by authors in discussion, however, we noted several issues in their article on which we invited the authors to comment.
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http://dx.doi.org/10.1111/aas.13963DOI Listing
July 2021

Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide- composite for wound dressing.

Carbohydr Polym 2021 Oct 2;269:118258. Epub 2021 Jun 2.

Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Shandong Provincial Key laboratory of Glycoscience and Glycoengineering, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China. Electronic address:

Marine polysaccharides or oligosaccharides have potential to promote wound healing due to their biocompatibility and physicochemical properties. However, microbial infection delays wound healing process, and novel antimicrobial wound dressings are urgently needed. Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA). Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm. Circular dichroism (CD) spectroscopy showed that AGO-AgNPs stabilized the α-helical structure of OA. AGO-AgNPs-OA showed stronger anti-bacterial activities than AGO-AgNPs, and had good biocompatibility and significant promoting effect on wound healing. Our data suggest that AMPs conjugated marine oligosaccharides and AgNPs may be effective and safe antibacterial materials for wound therapy.
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http://dx.doi.org/10.1016/j.carbpol.2021.118258DOI Listing
October 2021

Comparing high-flow nasal oxygenation or standard oxygenation for gastrointestinal endoscopy with sedation. Comment on Br J Anaesth 2021; 127: 133-42.

Br J Anaesth 2021 Jul 8. Epub 2021 Jul 8.

Department of Anaesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

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http://dx.doi.org/10.1016/j.bja.2021.06.017DOI Listing
July 2021

Association between postoperative delirium and cerebral oxygen desaturation in older patients undergoing cardiac surgery. Comment on Br J Anaesth 2020; 124: 146-53.

Br J Anaesth 2021 Jul 7. Epub 2021 Jul 7.

Department of Anaesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

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http://dx.doi.org/10.1016/j.bja.2021.06.019DOI Listing
July 2021

A meta-analysis for Lianhua Qingwen on the treatment of Coronavirus disease 2019 (COVID-19).

Complement Ther Med 2021 Aug 19;60:102754. Epub 2021 Jun 19.

The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, China. Electronic address:

Background: Traditional Chinese medicine (TCM) has been proven to played a great important role on the treatment of COVID-19. As one of the drugs recommended in Chinese guidelines, Lianhua Qingwen Granules or Capsules (LQ) are widely used.This systematic review and meta-analysis amis to evaluate the clinical efficacy of LQ on the treatment of COVID-19.

Methods: Seven databases (PubMed, EMBASE, CENTRAL, CNKI, VIP, CBM and Wanfang) were searched to include all appropriate clinical trials that explore the efficacy of LQ on the treatment of COVID-19.

Result: A total of 3 trials including 245 COVID-19 patients were eventually enrolled.Compared with the control group,the LQ group showed great significant difference on reducing the rate of clinical change to severe or critical condition[RR = 0.38, 95 %CI (0.17,0.85), P < 0.05]and the fever time (SMD =-0.57,95 %CI (-0.96,-0.17), P<0.05),as well as the significant improvement on the disappearance rate of the clinical symptoms: fever [RR = 1.36,95 %CI (1.14,1.61), P < 0.05],cough[RR = 1.99,95 %CI (1.39,2.86), P < 0.05],fatigue[RR = 1.52,95 %CI (1.15,2.01), P < 0.05] and anhelation [RR = 4.18,95 %CI (1.99,8.81), P < 0.05], but no significance on expectoration[RR = 2.46,95 %CI (0.81,7.51), P < 0.05].

Conclusion: The clinical application of LQ on the treatment of COVID-19 has significant efficacy in improving clinical symptoms and reducing the rate of clinical change to severe or critical condition. Nevertheless, due to the limited quantity and quality of the included studies, more and higher quality trials with more observational indicators are expected to be published.
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http://dx.doi.org/10.1016/j.ctim.2021.102754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213518PMC
August 2021

Genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from GI-1, GI-13, GI-28, and GI-19 strains in Southwestern China.

Poult Sci 2021 Jul 29;100(7):101210. Epub 2021 Apr 29.

Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, China. Electronic address:

Avian infectious bronchitis (IB), caused by avian infectious bronchitis virus (IBV), is an acute and highly contagious disease that is extremely harmful to the poultry industry throughout the world. The cross-using of different attenuated live vaccine strains has led to the occurrence of diverse IBV serotypes. In this study, we isolated an IBV strain from a chicken farm in southwest China and designated it CK/CH/SCMY/160315. Construction of a phylogenetic tree based on full S1 gene sequence analysis suggested that CK/CH/SCMY/160315 bears similarity to GI-28, and further comparison of S1 amino acid residues revealed that CK/CH/SCMY/160315 showed mutations and deletions in many key positions between LDT3-A and other GI-28 reference strains. Importantly, CK/CH/SCMY/160315 was identified as a novel recombinant virus derived from live attenuated vaccine strains H120 (GI-1), 4/91 (GI-13), LDT3-A (GI-28), and the field strain LJL/08-1 (GI-19), identifying at least 5 recombination sites in both structural and accessory genes. Pathogenicity analysis indicated that CK/CH/SCMY/160315 caused listlessness, sneezing, huddling, head shaking, and increased antibody levels in the inoculated chickens. To further describe pathogenicity of this novel strain, we assessed viral load in different tissues and conducted hematoxylin and eosin (HE) staining of the trachea, lungs and kidneys. Our results provide evidence for the continuing evolution of IBV field strains via genetic recombination and mutation, leading to outbreaks in the vaccinated chicken populations in China.
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http://dx.doi.org/10.1016/j.psj.2021.101210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192866PMC
July 2021

Genome-wide identification of U-box genes and protein ubiquitination under PEG-induced drought stress in potato.

Physiol Plant 2021 Jun 10. Epub 2021 Jun 10.

Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China.

Protein ubiquitination is one of the most important posttranslational modifications in eukaryotic cells, and it is involved in a variety of biological processes, including abiotic stress response. The ubiquitination modification is highly specific, which depends on the accurate recognition of substrate proteins by ubiquitin ligase. Plant U-box (PUB) proteins are a class of ubiquitin ligases, multiple members of which have shown to participate in water-deficit stress in Arabidopsis and rice. U-box gene family and large-scale profiling of the ubiquitome in potato has not been reported to date, although it is one of the most important food crops. The identified 66 U-box genes from the potato genome database were unevenly distributed on 10 chromosomes. These StPUBs have a large number of tandem repeat sequences. Analysis of gene expression characteristics revealed that many StPUBs responded to abiotic stress. Three hundred and fourteen lys modification sites were identified under PEG-induced drought stress, which were distributed on 200 proteins, with 25 differential ubiquitination modification sites, most of which were up-regulated. The ubiquitination modification in potato protein was enhanced under PEG-induced drought stress, and U-box ubiquitin ligase was involved. This study provides an overall strategy and rich data set to clarify the effects of ubiquitination on potatoes under PEG-induced drought stress and the ubiquitination modification involved in potato U-box genes in response to PEG-induced drought stress.
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http://dx.doi.org/10.1111/ppl.13475DOI Listing
June 2021

Comparing standard and non-standard videolaryngoscopes in children: methodological issues.

Br J Anaesth 2021 08 21;127(2):e52-e54. Epub 2021 May 21.

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

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http://dx.doi.org/10.1016/j.bja.2021.04.019DOI Listing
August 2021

Efficacy and safety of Naoxintong capsule for treating chronic stable angina: study protocol for a randomized controlled trial.

Trials 2021 May 10;22(1):336. Epub 2021 May 10.

The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, 510006, Guangdong Province, China.

Background: Cardiovascular disease is the leading cause of mortality and morbidity worldwide, Chronic stable angina (CSA) is the main symptom of myocardial ischemia, causes increased risk of major cardiovascular events such as sudden cardiac death and myocardial infarction. Naoxintong (NXT) capsule is a classical traditional Chinese medication used to treat CSA, however, few evidence to support the wide utility of NXT capsule for the treatment of CSA. We design this study to evaluate the efficacy and safety of NXT capsule versus placebo in patients with CSA.

Methods/design: This is a multicenter, randomized, double-blind, placebo-controlled clinical trial. A total of 260 eligible participants will be enrolled. The participants will be randomized assigned in an equal ratio to groups receiving either NXT or placebo for 12 weeks. After a 2-week run-in period, they will receive either NXT or placebo (3 pills, 3 times daily) for 12 weeks. The primary outcome is therapeutic efficacy. Secondary outcome measures include the quantitative score of TCM syndromes, severity grading of angina pectoris, the number of angina pectoris per week, nitroglycerin dosage, score of Seattle angina scale, serum homocysteine, and incidence of cardiovascular events. Safety outcomes and adverse events will be monitored throughout the trial.

Discussion: We designed this study in accordance with principles and regulations issued by the China Food and Drug Administration (CFDA). The results will provide clinical evidence of the efficacy and safety of NXT Capsule in the treatment of CSA.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2100044563 . Registered on 24 March 2020.
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http://dx.doi.org/10.1186/s13063-021-05264-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112004PMC
May 2021

Determining associations between preoperative brain MRI features and occurrence of postoperative delirium.

J Psychosom Res 2021 Jul 30;146:110505. Epub 2021 Apr 30.

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

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http://dx.doi.org/10.1016/j.jpsychores.2021.110505DOI Listing
July 2021

Detection of Mitochondrial Mutations Through Isothermal Nucleic Acid Amplification Coupled With Clustered Regularly Interspaced Short Palindromic Repeat-Associated Endonuclease Cas13a.

Front Genet 2020 12;11:622671. Epub 2021 Jan 12.

Hangzhou Matridx Biotechnology Co., Ltd., Hangzhou, China.

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated endonuclease Cas13a can specifically bind and cleave RNA. After nucleic acid pre-amplification, bacterial Cas13a has been used to detect genetic mutations. In our study, using a transcription-mediated amplification together with Cas13a, we can isothermally amplify and detect mitochondrial point mutations under non-denaturing conditions from human genomic DNA. Unlike previous reports, we prepared CRISPR DNA with T7 promoter sequences and generated CRISPR RNA transcription-mediated amplification instead of synthesizing and adding CRISPR RNA in a separate step. As a proof-of-concept, we showed that both m.1494C > T and m.1555A > G mutations were detected within 90 min. In addition, we explored various designs of CRISPR DNA to improve assay specificity, including the location and number of nucleotide mismatches, length of protospacer sequence, and different buffering conditions. We also confirmed the possibility of a "one-step single-tube" reaction for mutation detection. This assay can robustly distinguish circular DNA templates that differ by a single nucleotide. It has the potential to be adapted for automated applications, such as the screening of mitochondrial diseases.
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http://dx.doi.org/10.3389/fgene.2020.622671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835798PMC
January 2021

Identification and analysis of long non-coding RNAs and mRNAs in chicken macrophages infected with avian infectious bronchitis coronavirus.

BMC Genomics 2021 Jan 20;22(1):67. Epub 2021 Jan 20.

Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, 610064, China.

Background: Avian infectious bronchitis virus (IBV) is a gamma coronavirus that severely affects the poultry industry worldwide. Long non-coding RNAs (lncRNAs), a subset of non-coding RNAs with a length of more than 200 nucleotides, have been recently recognized as pivotal factors in the pathogenesis of viral infections. However, little is known about the function of lncRNAs in host cultured cells in response to IBV infection.

Results: We used next-generation high throughput sequencing to reveal the expression profiles of mRNAs and lncRNAs in IBV-infected HD11 cells. Compared with the uninfected cells, we identified 153 differentially expressed (DE) mRNAs (106 up-regulated mRNAs, 47 down-regulated mRNAs) and 181 DE lncRNAs (59 up-regulated lncRNAs, 122 down-regulated lncRNAs) in IBV-infected HD11 cells. Moreover, gene ontology (GO) and pathway enrichment analyses indicated that DE mRNAs and lncRNAs were mainly involved in cellular innate immunity, amino acid metabolism, and nucleic acid metabolism. In addition, 2640 novel chicken lncRNAs were identified, and a competing endogenous RNA (ceRNAs) network centered on gga-miR-30d and miR-146a-5p was established.

Conclusions: We identified expression profiles of mRNAs and lncRNAs during IBV infection that provided new insights into the pathogenesis of IBV.
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http://dx.doi.org/10.1186/s12864-020-07359-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816148PMC
January 2021

Survival prediction of patients suffering from glioblastoma based on two-branch DenseNet using multi-channel features.

Int J Comput Assist Radiol Surg 2021 Feb 18;16(2):207-217. Epub 2021 Jan 18.

Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, 211106, China.

Purpose: As the most common primary intracranial tumor, glioblastoma (GBM) is a malignant tumor that originated from neuroepithelial tissue, accounting for 40-50% of brain tumors. Precise survival prediction for patients suffering from GBM can not only help patients and doctors formulate treatment plans, but also help researchers understand the development of the disease and stimulate medical development.

Methods: In view of the tedious process of manual feature extraction and selection in traditional radiomics, we propose an end-to-end survival prediction model based on DenseNet to extract the features of magnetic resonance images including T1-weighted post-contrast images and T2-weighted images through two-branch networks. After segmenting the region of interest, the original image, the image of tumor region and the image without tumor are combined as input sample sets with three channels. Additionally, for some patients having only one of T1- or T2-weighted images, One2One CycleGAN is used to generate the T1 image from the T2 image, or vice versa. Flipping and rotating are also used for sample augmentation.

Result: By using the augmented training sample set to train the model, the classification and prediction accuracy of the two-branch DenseNet survival prediction model can reach up to 94%, and the Kaplan-Meier survival curve indicates that the model can classify patients into high-risk group and low-risk group based on whether they could survive for more than three years.

Conclusion: The classification and prediction results of the model and the survival analysis demonstrate that our model can get superior classification results which can be referenced by doctors and patients' families for developing medical plans. However, improving the loss function and expanding the sample size can further improve the prediction results, which are the target of our subsequent research.
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http://dx.doi.org/10.1007/s11548-021-02313-4DOI Listing
February 2021

Estimation of effective dose of propofol mono-sedation for successful insertion of upper gastrointestinal endoscope in healthy, non-obese Chinese adults.

J Clin Pharm Ther 2021 Apr 20;46(2):484-491. Epub 2020 Nov 20.

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

What Is Known And Objective: Propofol is effective in sedation for upper gastrointestinal (UGI) endoscopy. However, the optimum dose is ill-defined. This study aimed to estimate the effective dose of propofol mono-sedation for successful endoscope insertion in healthy, non-obese Chinese adults undergoing single UGI endoscopy.

Methods: Twenty-six adult patients undergoing elective single UGI endoscopy were enrolled in this study. A modified Dixon's up-and-down method was utilized to assess the effective dose of propofol for successful endoscope insertion. The initial dose of propofol administered, 1.6 mg/kg, was adjusted with 0.1 mg/kg as a step size. The patient's responses to endoscope insertion were classified as either 'movement' or 'no movement'. When patient's responses were changed from 'movement' to 'no movement' or from 'no movement' to 'movement', a crossover was defined. After eight crossovers had been obtained, patient recruitment was stopped. The mean of midpoints of all crossovers obtained by the modified Dixon's up-and-down method in all 26 patients was defined as calculated median effective dose (ED ) of propofol for successful endoscope insertion. Furthermore, probit regression analysis was used to determine the dose of propofol where 50% (ED ) and 95% (ED ) of endoscope insertion attempts were successful.

Results: The calculated ED of propofol for successful endoscope insertion was 1.89 ± 0.12 mg/kg. The probit regression analysis showed that ED and ED of propofol for successful endoscope insertion were 1.90 mg/kg (95% CI, 1.78-2.10 mg/kg) and 2.15 mg/kg (95% CI, 2.01-3.56 mg/kg), respectively. No any patient had hypoxaemia and gag reflex during the UGI endoscopy with propofol mono-sedation.

What Is New And Conclusion: In healthy, non-obese Chinese adults, propofol mono-sedation can provide excellent conditions of UGI endoscopy and the estimated ED of propofol for successful endoscope insertion is 1.89 ± 0.12 mg/kg.
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http://dx.doi.org/10.1111/jcpt.13312DOI Listing
April 2021

Comment on "Acute Kidney Injury After Esophageal Cancer Surgery: Incidence, Risk Factors, and Impact on Oncologic Outcomes".

Ann Surg 2020 Nov 17. Epub 2020 Nov 17.

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

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http://dx.doi.org/10.1097/SLA.0000000000004614DOI Listing
November 2020

[Continuous expression and functional prediction of circular RNA in mouse lung development].

Zhongguo Dang Dai Er Ke Za Zhi 2020 Oct;22(10):1125-1130

Neonatal Medical Center, Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China.

Objective: To study the continuous expression and potential function of circular RNA (circRNA), circ4:150439343|150477468 and circ15:73330849|73343359, in mouse lung development.

Methods: According to the stage of lung development, lung tissue samples were collected from mice on embryonic day 16.5 (E16.5), embryonic day 18.5 (E18.5), and postnatal day 2 (P2). Hematoxylin and eosin staining was performed to observe the morphology of lung tissue. Quantitative real-time PCR (qRT-PCR) was used to measure the mRNA expression of circ4:150439343|150477468 and circ15:73330849|73343359 during late lung development; miRanda and TargetScan were used to predict the target miRNAs of circRNAs, and then GO and KEGG analysis was performed for the target genes to predict the potential function of circRNAs.

Results: Type II alveolar epithelial cells were observed in the lung slices of E16.5 mice, with a gradual increase in number. On P2, the pulmonary alveoli expanded rapidly, the pulmonary interstitium became thinner, and the alveolar structure gradually became mature. The results of qRT-PCR showed that the relative expression of circ4:150439343|150477468 was continuously upregulated over time and the relative expression of circ15:73330849|73343359 was first downregulated and then upregulated (P<0.05). The KEGG and GO analysis showed that circRNAs were involved in the Notch, PI3K-Akt, and NF-κB signaling pathways.

Conclusions: Circ4:150439343|150477468 and circ15:73330849|73343359 can participate in lung development through the Notch signaling pathway.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568991PMC
October 2020

Potato E3 ubiquitin ligase PUB27 negatively regulates drought tolerance by mediating stomatal movement.

Plant Physiol Biochem 2020 Sep 18;154:557-563. Epub 2020 Jul 18.

Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070, People's Republic of China. Electronic address:

The ubiquitin-proteasome system (UPS) is one of the main ways of eukaryotic protein degradation and post-translational modification. It has proven as an essential process for plants to respond to abiotic stresses. Plant U-box (PUB) protein acts as a ubiquitin ligase, which recognizes and ubiquitinates the target proteins. Many PUBs have been involved in water stress in Arabidopsis and rice, but similar comprehensive studies in potato remained limited. In this study, the overexpressed and interfered transgenic potato plants of StPUB27 were obtained and their performances were evaluated under osmotic stress. The result showed that overexpression of StPUB27 accelerated the dehydration of detached leaves companied with greater stomatal conductance, while the down-regulated StPUB27 expression by RNA interference (RNAi) showed a smaller stomatal conductance and a lower rate of water loss in detached leaves, thus showing higher tolerance to osmotic stress. In addition, no significant changes in the proline content were observed between StPUB27 overexpressed and RNAi potato plants. The result demonstrated that potato E3 ubiquitin ligase PUB27 may negatively regulate drought tolerance by mediating stomatal conductance.
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http://dx.doi.org/10.1016/j.plaphy.2020.07.026DOI Listing
September 2020

PHOTO-SENSITIVE LEAF ROLLING 1 encodes a polygalacturonase that modifies cell wall structure and drought tolerance in rice.

New Phytol 2021 01 23;229(2):890-901. Epub 2020 Sep 23.

Department of Plant & Microbial Biology, University of California, 111 Koshland Hall, Berkeley, CA, 94720, USA.

The biosynthesis and modification of cell wall composition and structure are controlled by hundreds of enzymes and have a direct consequence on plant growth and development. However, the majority of these enzymes has not been functionally characterised. Rice mutants with leaf-rolling phenotypes were screened in a field. Phenotypic analysis under controlled conditions was performed for the selected mutant and the relevant gene was identified by map-based cloning. Cell wall composition was analysed by glycome profiling assay. We identified a photo-sensitive leaf rolling 1 (psl1) mutant with 'napping' (midday depression of photosynthesis) phenotype and reduced growth. The PSL1 gene encodes a cell wall-localised polygalacturonase (PG), a pectin-degrading enzyme. psl1 with a 260-bp deletion in its gene displayed leaf rolling in response to high light intensity and/or low humidity. Biochemical assays revealed PG activity of recombinant PSL1 protein. Significant modifications to cell wall composition in the psl1 mutant compared with the wild-type plants were identified. Such modifications enhanced drought tolerance of the mutant plants by reducing water loss under osmotic stress and drought conditions. Taken together, PSL1 functions as a PG that modifies cell wall biosynthesis, plant development and drought tolerance in rice.
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http://dx.doi.org/10.1111/nph.16899DOI Listing
January 2021

Genomic Analysis of the SUMO-Conjugating Enzyme and Genes under Abiotic Stress in Potato ( L.).

Int J Genomics 2020 24;2020:9703638. Epub 2020 Jun 24.

College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.

SUMO-conjugating enzymes (SCE) and SUMO (Small Ubiquitin-Like Modifiers) genes are important components of SUMOylation. SCE has a crucial role during the SUMOylation process which acts as a catalyst to transfer SUMO to the target protein. Comprehensive studies on SCE and SUMO have been performed in some plants, but studies on these genes remain limited in potato. This study is aimed at exploring the role of and genes in abiotic stress conditions. Nine and seven putative s and genes, respectively, were identified using different methods and databases available for potato. Chromosomal localization showed that and genes are unevenly distributed on 7 different chromosomes. Potato genome database was accessed for the expression profile of and genes, and these genes were differentially expressed in different tissues and organs during different phases of plant growth. The expression patterns on different treatments were further evaluated using qRT-PCR for all the and St genes. The expression was upregulated in and under salt and PEG treatment. and were upregulated under salt stress whereas and and were observed downregulated under PEG treatment. The results of this study could be useful to explore the role of genes in potato improvement.
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http://dx.doi.org/10.1155/2020/9703638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335410PMC
June 2020

Guipi decoction for coronary heart disease: A protocol for a systematic review and meta-analysis.

Medicine (Baltimore) 2020 Aug;99(32):e21589

The Second Clinical Medical School, Guangzhou University of Chinese Medicine.

Background: Coronary heart disease (CHD) is the leading cause of morbidity and mortality worldwide. Guipi Decoction (GPD) is a classical traditional Chinese medication used to treat CHD. However, systematic review and meta-analysis regarding its efficacy and safety has not been systematically evaluated. The objective of this protocol is to determine the efficacy and safety of GPD in the treatment of CHD.

Methods: Randomized controlled trials evaluating the effectiveness and safety of GPD in the treatment of CHD will be retrieved from 8 electronic databases, including PubMed, EMBASE, Cochrane Library, Web of science, China National Knowledge Infrastructure Database, VIP Database, Wanfang Database and China Biology Medicine Database. Study selection, data collection, risk of bias assessment, and evaluation of the quality of evidence will be performed in order. Data will be analyzed by RevMan V.5.3.5 software.

Results: This study will evaluate the efficacy and safety of GPD in the treatment of CHD.

Conclusion: This systematic review will provide evidence for determining whether or not GPD is an effective and safe intervention for CHD.

Prospero Registration Number: PROSPERO CRD 42020156420.
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http://dx.doi.org/10.1097/MD.0000000000021589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593082PMC
August 2020

Effect of diabetes mellitus on survival in patients with gallbladder Cancer: a systematic review and meta-analysis.

BMC Cancer 2020 Jul 24;20(1):689. Epub 2020 Jul 24.

School of Nursing and Health, Nanfang College of Sun Yat-sen University, Guangzhou, 510970, Guangdong Province, China.

Background: Increasing evidences indicated that diabetes might increase the incidence of gallbladder cancer. However, no sufficient data has ever clarified the impact of diabetes on the survival of patients with gallbladder cancer.

Methods: We comprehensively searched PubMed, Embase, and the Cochrane Library databases through July 2019 in order to find sufficient eligible researches. The pooled hazard risks (HRs) and relative risks (RRs) with 95% confidence intervals (CIs) were calculated with either fix-effects or random-effects model. Due to the low gallbladder cancer mortality in general population, the RRs and standard mortality ratios (SMRs) were considered the similar estimates of the HRs.

Results: Ten eligible studies were included in this meta-analysis. Analysis of eight cohorts found that diabetes was closely associated with the mortality of gallbladder cancer (HR = 1.10; 95% CI: 1.06-1.14; P < 0.00001). However, the mortality in male diabetes patients was not higher than female patients (RR = 1.08, 95%CI = 0.57-2.04, P = 0.80).

Conclusions: These findings indicated that diabetes patients had a higher mortality of gallbladder cancer compared with non-diabetes.
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http://dx.doi.org/10.1186/s12885-020-07139-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7379826PMC
July 2020

Antimicrobial Resistance, Virulence Genes, and Biofilm Formation Capacity Among From Yaks in Aba Tibetan Autonomous Prefecture, China.

Front Microbiol 2020 12;11:1250. Epub 2020 Jun 12.

Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.

Yaks provide necessities such as meat and milk for Tibetans living at high altitudes on and around the Qinghai-Tibetan Plateau. Enterococci are ubiquitous members of the animal gut microbiota that can cause biofilm-associated opportunistic infections. Meanwhile, multidrug-resistant also poses a serious threat to public health. This study aims to characterize antibiotic resistance, virulence genes, and biofilm formation of enterococci from yaks. From April 2018 to July 2019, we collected 395 fecal samples of yaks in Aba Tibetan Autonomous Prefecture, China. Enterococci isolated from the samples were identified and classified according to the 16S rDNA sequence. The antibiotic resistance of each isolate was detected according to the Kirby-Bauer disk diffusion method, and antibiotic resistance genes were detected by polymerase chain reaction (PCR) and sequencing. Enterococcal biofilms were assessed using standard procedures. Different virulence genes were detected by PCR and sequencing. In total, 381 enterococci strains were recovered, with (41.99%) and (37.80%) being the predominant species. Many isolates were multidrug- resistant (60.37%) and showed a high resistance rate to rifampicin (64.30%) and tetracycline (61.54%). We also detected various antimicrobial resistance (AMR) genes in the tested strains. The strains had higher frequency of biofilm formation and virulence genes than other enterococcal species. This is the first report that shows yaks are repositories for drug-resistant enterococci with virulent determinants and biofilms that may spread into humans and to environment. This study also provides useful data suggesting that enterococci may pose a potential health risk to yaks. Therefore, active surveillance of AMR and pathogenesis in enterococci from yaks is urgently warranted.
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http://dx.doi.org/10.3389/fmicb.2020.01250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304059PMC
June 2020

A recombinase polymerase amplification combined with lateral flow dipstick for rapid and specific detection of African swine fever virus.

J Virol Methods 2020 11 31;285:113885. Epub 2020 May 31.

Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, PR China. Electronic address:

African swine fever (ASF) is an acute, hemorrhagic, highly contagious disease caused by African swine fever virus (ASFV) infection of domestic pigs and wild boars, showing mortality rates up to 100 %. There are no effective vaccines or antiviral drugs available for ASFV. Therefore, disease control is mainly based on animal slaughtering and the enforcement of strict sanitary measures. In order to establish a rapid, sensitive and simple method for on-site detection of ASFV, a recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD) was developed using a pair of specific primers and probe. Using recombinant plasmid pMD19-T-K205R DNA as a template, the RPA-LFD detection could be accomplished in 10 min at a temperature of 36℃-44℃. More specific than PCR and more rapid and simpler than real-time PCR, RPA-LFD has the same detection limit of 1 × 10 copies/reaction as real-time PCR, also with no cross-reaction with other viral strains. A convenient and rapid ASFV RPA-LFD detection method was developed, which will provide an efficient method for investigating epidemiology of ASFV infection.
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http://dx.doi.org/10.1016/j.jviromet.2020.113885DOI Listing
November 2020

The Ubiquitin Conjugating Enzyme: An Important Ubiquitin Transfer Platform in Ubiquitin-Proteasome System.

Int J Mol Sci 2020 Apr 21;21(8). Epub 2020 Apr 21.

College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.

Owing to a sessile lifestyle in nature, plants are routinely faced with diverse hostile environments such as various abiotic and biotic stresses, which lead to accumulation of free radicals in cells, cell damage, protein denaturation, etc., causing adverse effects to cells. During the evolution process, plants formed defense systems composed of numerous complex gene regulatory networks and signal transduction pathways to regulate and maintain the cell homeostasis. Among them, ubiquitin-proteasome pathway (UPP) is the most versatile cellular signal system as well as a powerful mechanism for regulating many aspects of the cell physiology because it removes most of the abnormal and short-lived peptides and proteins. In this system, the ubiquitin-conjugating enzyme (E2) plays a critical role in transporting ubiquitin from the ubiquitin-activating enzyme (E1) to the ubiquitin-ligase enzyme (E3) and substrate. Nevertheless, the comprehensive study regarding the role of E2 enzymes in plants remains unexplored. In this review, the ubiquitination process and the regulatory role that E2 enzymes play in plants are primarily discussed, with the focus particularly put on E2's regulation of biological functions of the cell.
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http://dx.doi.org/10.3390/ijms21082894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215765PMC
April 2020

Assessing Association Between Type of Intraoperative Blood Transfusion and Postoperative Delirium.

Spine (Phila Pa 1976) 2020 05;45(10):E605

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

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http://dx.doi.org/10.1097/BRS.0000000000003488DOI Listing
May 2020

Gga-miR-30d regulates infectious bronchitis virus infection by targeting USP47 in HD11 cells.

Microb Pathog 2020 Apr 23;141:103998. Epub 2020 Jan 23.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, PR China. Electronic address:

Avian infectious bronchitis virus (IBV) is a coronavirus which infects chickens and causes severe economic losses to the poultry industry worldwide. MicroRNAs (miRNAs) are important intracellular regulators and play a pivotal role in viral infections. In previous studies, we have revealed that IBV infection caused a significant down-regulation of gga-miR-30d expression in chicken kidneys. In present study, we investigated the role of gga-miR-30d in the process of IBV infection of HD11 cell line in vitro. By transfecting the mimics and inhibitor of gga-miR-30d, it was found that overexpressed gga-miR-30d inhibited IBV replication. Contrarily, low-expressed gga-miR-30d promoted IBV replication. In addition, dual-luciferase reporter assays revealed that ubiquitin-specific protease 47 (USP47), a deubiquitinase-encoding gene, was a target for gga-miR-30d. This is the first study demonstrating that miRNAs regulate IBV replication by regulating the deubiquitinating enzyme (DUBs).
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http://dx.doi.org/10.1016/j.micpath.2020.103998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125550PMC
April 2020

A potato RING-finger protein gene StRFP2 is involved in drought tolerance.

Plant Physiol Biochem 2020 Jan 30;146:438-446. Epub 2019 Nov 30.

College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China; Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070, China. Electronic address:

The ubiquitin/26S proteasome pathway is widely related to plant growth and metabolism and response to treatment by specifically degrading ubiquitin-modified proteins, including RING-finger-type E3 ubiquitin ligase (RING). The RING finger protein (RFP) gene family, determining the specificity of the ubiquitination process, is numerous and complex in function. In this study, we constructed a pCEGFP-StRFP2 fusion protein expression vector and transformed it into tobacco to achieve transient expression, thereby confirming that StRFP2 is localized in the cell membrane and cytoplasm. The result of qRT-PCR analysis showed that StRFP2 gene was significantly expressed in potato leaves, and the expression level of StRFP2 was significantly up-regulated under drought treatment. The transgenic plants of overexpressing StRFP2 gene were obtained with Agrobacterium tumefaciens-mediated transformation. Plant height, stem diameter, root length, fresh weight and root-shoot ratio of transgenic plants were significantly higher than those of non-transgenic plants (WT), indicating that the growth of plants was significantly promoted after overexpression of StRFP2 gene. Under PEG osmotic stress, the expressional level of StRFP2 in transgenic potato plants was significantly higher than that of WT. Furthermore, the free proline content and CAT activity in transgenic plants were higher than WT, on the contrary, MDA was lower than WT, and transgenic plants have stronger water retention capacity under simulated drought stress treatment, which indicated that StRFP2 could strengthen the tolerance of plants responding to drought stress. The above evidence strongly suggested that the StRFP2 gene is obviously up-regulated expression by drought stress, thereby enhancing the drought tolerance of the potato.
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http://dx.doi.org/10.1016/j.plaphy.2019.11.042DOI Listing
January 2020

miR-129-5p Inhibits Adipogenesis through Autophagy and May Be a Potential Biomarker for Obesity.

Int J Endocrinol 2019 6;2019:5069578. Epub 2019 Nov 6.

Department of Endocrinology and Metabolism, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Introduction: Obesity has an unclear pathogenesis. MicroRNAs (miRNAs) may function as biologically active molecules for obesity through regulating adipocyte differentiation. This study aimed to identify how miR-129-5p (a specific miRNA) regulates adipogenesis in vitro and explore its possible role in the pathogenesis of obesity in humans.

Materials And Methods: The miR-129-5p expression was detected in obese mouse models. The effect of miR-129-5p on adipocyte differentiation was observed, and the adipose markers were analyzed. Bioinformatics and dual-luciferase reporter assay were applied to predict and confirm the target genes of miR-129-5p. The human serum samples were detected and analyzed.

Results: miR-129-5p is highly expressed in adipose tissues of mice. Gain- and loss-of-function studies show that miR-129-5p could significantly inhibit adipocyte differentiation and white adipocyte browning in vitro and decreases the level of specific markers, such as FABP4, UCP1, and PPAR, in mature white and brown adipocytes. miR-129-5p directly targets ATG7 which is predicted with bioinformatics and confirmed by dual-luciferase reporter assay. Serum miR-129-5p level was evidently elevated in patients with simple obesity ( < 0.01) and correlates with obesity indices, including BMI ( = 0.407, < 0.029) and fat percentage ( = 0.394, < 0.038).

Conclusion: miR-129-5p might target on the ATG7-related autophagy signaling network that regulates white and brown adipogenesis. Importantly, the aforementioned results suggest serum miR-129-5p might be a potential biomarker and therapeutic target for obesity.
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http://dx.doi.org/10.1155/2019/5069578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875017PMC
November 2019
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