Publications by authors named "Xue Bai"

1,123 Publications

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Influence of zinc levels and Nrf2 expression in the clinical and pathological changes in patients with diabetic nephropathy.

Nutr Diabetes 2022 Aug 6;12(1):37. Epub 2022 Aug 6.

Department of Nephropathy, The Second Hospital of Jilin University, Changchun, Jilin Province, China.

Objective: We investigated the correlation between zinc levels and Nrf2 expression and potential effects on the clinicopathology of patients with diabetic nephropathy (DN).

Methods: We selected 30 patients with DN, diagnosed via renal biopsy at our hospital from March 2018 to November 2019, and enrolled 30 healthy individuals from a medical examination center as the control group. Patients with DN were divided into normal-zinc and low-zinc groups. We detected the levels of zinc, copper, and Nrf2 mRNA in their serum, and collected the clinical and pathological data of DN patients.

Results: Serum zinc level and Nrf2 mRNA expression were significantly decreased in patients with DN compared to those of healthy people (P < 0.05). Of the 30 patients, 16 had low zinc (53.3%) and 14 had normal zinc levels (46.7%). There was no significant difference in the blood Nrf2 mRNA expression between the two groups (P > 0.05). However, the expression of Nrf2 in the kidney tissue of the low-zinc group was significantly lower compared to the normal-zinc group (P < 0.05). Diastolic blood pressure and copper levels were significantly higher in the low-zinc group (P < 0.05). In contrast, body mass index, red blood cell count, Hb level, and the ratio of zinc to copper were significantly lower in the low-zinc group (P < 0.05). The pathological classifications of the low-zinc group were more severe (P < 0.05).

Conclusion: Patients with DN were more likely to have zinc deficiency and lower expression of Nrf2. Additionally, DN patients with zinc deficiency were prone to have more severe clinical and pathological manifestations.
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http://dx.doi.org/10.1038/s41387-022-00212-4DOI Listing
August 2022

In vitro magnetosome remineralization for silver-magnetite hybrid magnetosome biosynthesis and used for healing of the infected wound.

J Nanobiotechnology 2022 Aug 6;20(1):364. Epub 2022 Aug 6.

School of Mechanical Engineering and Automation, Beihang University, Beijing, 100083, China.

Background: Magnetosomes (BMPs) are organelles of magnetotactic bacteria (MTB) that are responsible for mineralizing iron to form magnetite. In addition, BMP is an ideal biomaterial that is widely used in bio- and nano-technological applications, such as drug delivery, tumor detection and therapy, and immunodetection. The use of BMPs to create multifunctional nanocomposites would further expand the range of their applications.

Results: In this study, we firstly demonstrate that the extracted BMP can remineralize in vitro when it is exposed to AgNO solution, the silver ions (Ag) were transported into the BMP biomembrane (MM) and mineralized into a silver crystal on one crystal plane of FeO. Resulting in the rapid synthesis of an Ag-FeO hybrid BMP (BMP-Ag). The synergy between the biomembrane, FeO crystal and unmineralized iron enabled the remineralization of BMPs at an Ag concentration ≥ 1.0 mg mL. The BMP-Ag displayed good biocompatibility and antibacterial activity. At a concentration of 2.0 mg/mL, the BMP-Ag and biomembrane removed Ag-FeO NPs inhibited the growth of gram-negative and gram-positive bacteria. Thus using BMP-Ag as a wound dressing can effectively enhance the contraction of infected wounds.

Conclusions: This study represents the first successful attempt to remineralize organelles ex vivo, realizing the biosynthesis of hybrid BMP and providing an important advancement in the synthesis technology of multifunctional biological nanocomposites.
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http://dx.doi.org/10.1186/s12951-022-01532-4DOI Listing
August 2022

Apatite nanosheets inhibit initial smooth muscle cell proliferation by damaging cell membrane.

Biomater Adv 2022 Jun 13;137:212852. Epub 2022 May 13.

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, PR China. Electronic address:

Understanding how nanostructured coatings interact with cells is related to how they manipulate cell behaviors and is therefore critical for designing better biomaterials. The apatite nanosheets were deposited on metallic substrates via biomimetic precipitation. Cell viability of apatite nanosheets towards to smooth muscle cells (SMCs) were investigated, and the underlying mechanism was proposed. Apatite nanosheets presented inhibitory activity on SMC growth, and caused rupture of cell membranes. On the basis of measuring changes in intracellular calcium ([Ca]), observing cell contraction and apatite nanosheets - SMC interaction, it was found that calcium ions released from apatite led to rises in [Ca], which induced vigorous SMC contraction on apatite nanosheets. Consequently, the cell membrane of individual SMCs was cut/penetrated by the sharp edges of apatite nanosheets, resulting in cell inactivation. This damage of cell membranes suggests a novel mechanism to manipulate cell viability, and may offer insights for the better design of calcium-based nanostructured coatings or other biomedical applications.
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http://dx.doi.org/10.1016/j.bioadv.2022.212852DOI Listing
June 2022

Modern research thoughts and methods on bio-active components of TCM formulae.

Chin J Nat Med 2022 Jul;20(7):481-493

Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drug Research, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, Shaanxi University of Chinese Medicine, Xi'an 712046, China.

TCM formulae are the important guidances for clinical application of traditional Chinese medicines, which follow the principles of diagnosis and treatment in TCM. Elucidating the bio-active components of TCM formulae is the key to the modernization and internationalization of traditional Chinese medicines. With the rapid development of modern instruments and technology, many new theories, methods and strategies are emerging, which upgrade the research of TCM formulae into a higher level. Only when the medicinal efficacy, bio-active components, function mechanism of TCM formulae are understood, we can guarantee TCM safety and quality control. In this paper, we summarized the latest modern research thoughts and methods on bio-active components of TCM formulae including formula decomposition study, serum pharmacology and serum pharmacochemistry, association analysis, biochromatography, network pharmacology, metabolomics and proteomics, so as to provide reference for the research and development of TCM in the future.
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http://dx.doi.org/10.1016/S1875-5364(22)60206-1DOI Listing
July 2022

Classification of Alzheimer's Disease Based on Deep Learning of Brain Structural and Metabolic Data.

Front Aging Neurosci 2022 12;14:927217. Epub 2022 Jul 12.

Department of Radiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

To improve the diagnosis and classification of Alzheimer's disease (AD), a modeling method is proposed based on the combining magnetic resonance images (MRI) brain structural data with metabolite levels of the frontal and parietal regions. First, multi-atlas brain segmentation technology based on T1-weighted images and edited magnetic resonance spectroscopy (MRS) were used to extract data of 279 brain regions and levels of 12 metabolites from regions of interest (ROIs) in the frontal and parietal regions. The -test combined with false discovery rate (FDR) correction was used to reduce the dimensionality in the data, and MRI structural data of 54 brain regions and levels of 4 metabolites that obviously correlated with AD were screened out. Lastly, the stacked auto-encoder neural network (SAE) was used to classify AD and healthy controls (HCs), which judged the effect of classification method by fivefold cross validation. The results indicated that the mean accuracy of the five experimental model increased from 96 to 100%, the AUC value increased from 0.97 to 1, specificity increased from 90 to 100%, and F1 value increased from 0.97 to 1. Comparing the effect of each metabolite on model performance revealed that the gamma-aminobutyric acid (GABA) + levels in the parietal region resulted in the most significant improvement in model performance, with the accuracy rate increasing from 96 to 98%, the AUC value increased from 0.97 to 0.99 and the specificity increasing from 90 to 95%. Moreover, the GABA + levels in the parietal region was significantly correlated with Mini Mental State Examination (MMSE) scores of patients with AD ( = 0.627), and the F statistics were largest ( = 25.538), which supports the hypothesis that dysfunctional GABAergic system play an important role in the pathogenesis of AD. Overall, our findings support that a comprehensive method that combines MRI structural and metabolic data of brain regions can improve model classification efficiency of AD.
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http://dx.doi.org/10.3389/fnagi.2022.927217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315355PMC
July 2022

Acute-on-chronic liver failure following eribulin treatment for metastatic breast cancer: a case report.

J Int Med Res 2022 Jul;50(7):3000605221090097

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 113 Baohe Road, Shenzhen, 518116, P.R. China.

The efficacy and tolerability of eribulin mesylate, a synthetic halichondrin B analog, in patients with metastatic breast cancer (MBC) previously treated with anthracyclines and taxanes have been established. Acute-on-chronic liver failure (ACLF) is a clinical syndrome manifesting as acute and severe hepatic derangement resulting from varied insults in patients with established chronic liver disease or cirrhosis who did not previously receive eribulin. A middle-aged woman diagnosed with MBC and diffuse liver metastases who was pretreated with multi-line chemotherapy received eribulin as eighth-line chemotherapy and presented with hepatic encephalopathy, rapid bilirubin elevation, and significant coagulation dysfunction on day 4 in cycle 1. The patient was diagnosed with ACLF induced by eribulin. Therefore, ACLF may be a lethal and rare adverse event when patients with chronic liver metastases receive eribulin treatment, and clinicians' awareness should be increased for optimal prevention and prompt diagnosis and treatment.
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http://dx.doi.org/10.1177/03000605221090097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340925PMC
July 2022

Near-Field High-Resolution SAR Imaging with Sparse Sampling Interval.

Sensors (Basel) 2022 Jul 25;22(15). Epub 2022 Jul 25.

School of Electrical and Information Engineering, Jiangsu University, Zhenjiang 212000, China.

Near-field high-resolution synthetic aperture radar (SAR) imaging is mostly accompanied by a large number of data acquisition processes, which increases the system complexity and device cost. According to extensive reports, reducing the number of sampling points of a radar in space can greatly reduce the amount of data. However, when spatial sparse sampling is carried out, a ghost normally appears in the imaging results due to the high side lobes generated in the azimuth. To address this issue, a technique is introduced in this paper to recover the blank data through amplitude and phase compensation based on the correlation between sparse array sampling through adjacent points. Firstly, the data sampled by the sparse array is compressed in the range direction to obtain the expected data slices in the same range direction. Then, the blank element of the slice is compensated for with amplitude and phase to obtain full aperture data. Finally, the matched filter method is used to aid in the image reconstruction. The simulation results verified that the method proposed in this paper can effectively reconstruct the image under two kinds of sparse sampling conditions. Thus, a simple single-input single-output (SISO) synthetic aperture radar imaging test bench is established. Compared with the results of a 1 mm (1/4 λ) sampling interval, the quality of the reconstructed image under the condition of a 4 mm (1 λ) sampling interval still stands using our proposed method. Demonstrated by the experiment, the normalized root-mean-square error(NMSE) is 5.75%. Additionally, when the spatial sampling points are sampled randomly with 30% of the full sampling condition, this method can also restore and reconstruct the image with high quality. Due to the decrease of sampling points, the data volume can be reduced, which is beneficial for improving the scanning speed and alleviating the pressure of data transmission for near-field high resolution SAR imaging systems.
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http://dx.doi.org/10.3390/s22155548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330117PMC
July 2022

Structural and Functional Analysis of the Pyridoxal Phosphate Homeostasis Protein YggS from .

Molecules 2022 Jul 26;27(15). Epub 2022 Jul 26.

Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, China.

Pyridoxal 5'-phosphate (PLP) is the active form of vitamin B6, but it is highly reactive and poisonous in its free form. YggS is a PLP-binding protein found in bacteria and humans that mediates PLP homeostasis by delivering PLP to target enzymes or by performing a protective function. Several biochemical and structural studies of YggS have been reported, but the mechanism by which YggS recognizes PLP has not been fully elucidated. Here, we report a functional and structural analysis of YggS from (FnYggS). The PLP molecule could bind to native FnYggS, but no PLP binding was observed for selenomethionine (SeMet)-derivatized FnYggS. The crystal structure of FnYggS showed a type III TIM barrel fold, exhibiting structural homology with several other PLP-dependent enzymes. Although FnYggS exhibited low (<35%) amino acid sequence similarity with previously studied YggS proteins, its overall structure and PLP-binding site were highly conserved. In the PLP-binding site of FnYggS, the sulfate ion was coordinated by the conserved residues Ser201, Gly218, and Thr219, which were positioned to provide the binding moiety for the phosphate group of PLP. The mutagenesis study showed that the conserved Ser201 residue in FnYggS was the key residue for PLP binding. These results will expand the knowledge of the molecular properties and function of the YggS family.
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http://dx.doi.org/10.3390/molecules27154781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332261PMC
July 2022

Photocatalytic Degradation of Some Typical Antibiotics: Recent Advances and Future Outlooks.

Int J Mol Sci 2022 Jul 24;23(15). Epub 2022 Jul 24.

Division of Pharmacy and Optometry, Faculty of Biology, Medicine and Health, School of Health Science, University of Manchester, Oxford Road, Manchester M13 9PT, UK.

The existence of antibiotics in the environment can trigger a number of issues by fostering the widespread development of antimicrobial resistance. Currently, the most popular techniques for removing antibiotic pollutants from water include physical adsorption, flocculation, and chemical oxidation, however, these processes usually leave a significant quantity of chemical reagents and polymer electrolytes in the water, which can lead to difficulty post-treating unmanageable deposits. Furthermore, though cost-effectiveness, efficiency, reaction conditions, and nontoxicity during the degradation of antibiotics are hurdles to overcome, a variety of photocatalysts can be used to degrade pollutant residuals, allowing for a number of potential solutions to these issues. Thus, the urgent need for effective and rapid processes for photocatalytic degradation leads to an increased interest in finding more sustainable catalysts for antibiotic degradation. In this review, we provide an overview of the removal of pharmaceutical antibiotics through photocatalysis, and detail recent progress using different nanostructure-based photocatalysts. We also review the possible sources of antibiotic pollutants released through the ecological chain and the consequences and damages caused by antibiotics in wastewater on the environment and human health. The fundamental dynamic processes of nanomaterials and the degradation mechanisms of antibiotics are then discussed, and recent studies regarding different photocatalytic materials for the degradation of some typical and commonly used antibiotics are comprehensively summarized. Finally, major challenges and future opportunities for the photocatalytic degradation of commonly used antibiotics are highlighted.
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http://dx.doi.org/10.3390/ijms23158130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331861PMC
July 2022

SMAD4, activated by the TCR-triggered MEK/ERK signaling pathway, critically regulates CD8 T cell cytotoxic function.

Sci Adv 2022 Jul 27;8(30):eabo4577. Epub 2022 Jul 27.

Institute for Immunology and School of Medicine, Tsinghua University, Beijing 100084, China.

Transforming growth factor-β is well known to restrain cytotoxic T cell responses to maintain self-tolerance and to promote tumor immune evasion. In this study, we have investigated the role of SMAD4, a core component in the TGF-β signaling pathway, in CD8 T cells. Unexpectedly, we found that SMAD4 was critical in promoting CD8 T cell function in both tumor and infection models. SMAD4-mediated transcriptional regulation of CD8 T cell activation and cytotoxicity was dependent on the T cell receptor (TCR) but not TGF-β signaling pathway. Following TCR activation, SMAD4 translocated into the nucleus, up-regulated genes encoding TCR signaling components and cytotoxic molecules in CD8 T cells and thus reinforced T cell function. Biochemically, SMAD4 was directly phosphorylated by ERK at Ser residue following TCR activation. Our study thus demonstrates a critical yet unexpected role of SMAD4 in promoting CD8 T cell-mediated cytotoxic immunity.
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http://dx.doi.org/10.1126/sciadv.abo4577DOI Listing
July 2022

A transcriptional regulatory network of HNF4α and HNF1α involved in human diseases and drug metabolism.

Drug Metab Rev 2022 Jul 26:1-25. Epub 2022 Jul 26.

Research Center for High Altitude Medicine, Qinghai University Medical College, Xining, China.

HNF4α and HNF1α are core transcription factors involved in the development and progression of a variety of human diseases and drug metabolism. They play critical roles in maintaining the normal growth and function of multiple organs, mainly the liver, and in the metabolism of endogenous and exogenous substances. The twelve isoforms of HNF4α may exhibit different physiological functions, and HNF4α and HNF1α show varying or even opposing effects in different types of diseases, particularly cancer. Additionally, the regulation of CYP450, phase II drug-metabolizing enzymes, and drug transporters is affected by several factors. This article aims to review the role of HNF4α and HNF1α in human diseases and drug metabolism, including their structures and physiological functions, affected diseases, regulated drug metabolism genes, influencing factors, and related mechanisms. We also propose a transcriptional regulatory network of HNF4α and HNF1α that regulates the expression of target genes related to disease and drug metabolism.
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http://dx.doi.org/10.1080/03602532.2022.2103146DOI Listing
July 2022

Effects of Environmental Concentrations of Total Phosphorus on the Plankton Community Structure and Function in a Microcosm Study.

Int J Environ Res Public Health 2022 Jul 9;19(14). Epub 2022 Jul 9.

Key Laboratory of Pollution Process and Environmental Criteria of Ministry of Education and Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.

The excessive nutrients in freshwater have been proven to promote eutrophication and harmful algae blooms, which have become great threats to water quality and human health. To elucidate the responses of the plankton community structure and function to total phosphorus (TP) at environmental concentrations in the freshwater ecosystem, a microcosm study was implemented. The results showed that plankton communities were significantly affected by the TP concentration ≥ 0.1 mg/L treatments. In terms of community structure, TP promoted the growth of Cyanophyta. This effect was transmitted to the zooplankton community, resulting in the promotion of Cladocera growth from day 42. The community diversities of phytoplankton and zooplankton had been continuously inhibited by TP. The principal response curve (PRC) analysis results demonstrated that the species composition of phytoplankton and zooplankton community in TP enrichment treatments significantly ( ≤ 0.05) deviated from the control. For community function, TP resulted in the decline in phytoplankton photosynthesis. The chlorophyll fluorescence parameters were significantly inhibited when TP concentration reached 0.4 mg/L. In TP ≥ 0.1 mg/L treatments, the reductions in total phytoplankton abundances led to a continuous decrease in pH. This study can directly prove that the plankton community changes significantly when TP concentrations are greater than 0.1 mg/L and can help managers to establish specific nutrient management strategies for surface water.
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http://dx.doi.org/10.3390/ijerph19148412DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324525PMC
July 2022

Hydrogen Sulfide Increases the Analgesic Effects of µ- and δ-Opioid Receptors during Neuropathic Pain: Pathways Implicated.

Antioxidants (Basel) 2022 Jul 4;11(7). Epub 2022 Jul 4.

Grup de Neurofarmacologia Molecular, Institut d'Investigació Biomèdica Sant Pau (IIB Sant Pau), 08041 Barcelona, Spain.

Recent studies have revealed that hydrogen sulfide (HS) increases the analgesic actions of the δ-opioid receptor (DOR) in inflammatory pain. However, the possible improvement of the analgesia of μ-opioid receptor (MOR) and DOR agonists during neuropathic pain, through pretreatment with two slow-releasing HS donors-DADS (diallyl disulfide) and GYY4137 (morpholin-4-ium 4-methoxyphenyl(morpholino) phosphinodithioate dichloromethane complex)-is still unknown. In male C57BL/6J mice with neuropathic pain incited by chronic constriction of the sciatic nerve (CCI), we evaluated: (1) the influence of DADS (3.5 mg/kg) and GYY4137 (0.7 mg/kg) on the inhibition of the allodynia and hyperalgesia produced by the systemic or local administration of morphine (3 mg/kg or 65 µg) and UFP-512 (1 mg/kg or 12.5 µg); (2) the reversion of the antinociceptive actions of high doses of DADS (30 mg/kg) and GYY4137 (24 mg/kg) with MOR and DOR antagonists; and (3) the effects of HS donors on oxidative stress, apoptotic responses, and MOR and DOR expression in the medial septum (MS) and dorsal root ganglia (DRG). The results revealed that both DADS and GYY4137 improved the antiallodynic effects of morphine and UFP-512, possibly by up-regulating MOR and DOR expression in DRG. The administration of MOR and DOR antagonists blocked the analgesic properties of DADS and GYY4137, revealing the feasible participation of the endogenous opioid system in HS analgesic effects. Moreover, both HS donors inhibited oxidative stress and apoptosis generated by CCI in the MS and/or DRG. This study suggests the co-treatment of HS donors with MOR or DOR agonists as a potential therapy for neuropathic pain.
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http://dx.doi.org/10.3390/antiox11071321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311550PMC
July 2022

The Interaction between Carbon Monoxide and Hydrogen Sulfide during Chronic Joint Pain in Young Female Mice.

Antioxidants (Basel) 2022 Jun 27;11(7). Epub 2022 Jun 27.

Grup de Neurofarmacologia Molecular, Institut d'Investigació Biomèdica Sant Pau (IIB Sant Pau), 08041 Barcelona, Spain.

A relationship between carbon monoxide (CO) and hydrogen sulfide (HS) has been described in different pathological conditions, but their interaction in modulating joint pain has not yet been investigated. In young female mice with monosodium acetate-induced joint degeneration and pain, we assessed: (1) the effects of CORM-2 (tricarbonyldichlororuthenium(II)dimer), a CO-releasing molecule, and CoPP (cobalt protoporphyrin IX), an inducer of heme oxygenase 1 (HO-1), administered alone and combined with low doses of two slow-releasing HS donors, DADS (diallyl disulfide) and GYY4137 (morpholin-4-ium 4-methoxyphenyl(morpholino) phosphinodithioate dichloromethane complex) on the mechanical allodynia and loss of grip strength provoked by joint degeneration; (2) the role of Nrf2, NAD(P)H: quinone oxidoreductase 1 (NQO1) and HO-1 in the antinociceptive actions of HS donors; (3) the impact of DADS and GYY4137 treatment on the expression of Nrf2 and several antioxidant proteins in dorsal root ganglia (DRG) and periaqueductal gray matter (PAG). Our data showed that treatment with HS donors inhibited allodynia and functional deficits, while CORM-2 and CoPP only prevented allodynia. The Nrf2 pathway is implicated in the analgesic actions of DADS and GYY4137 during joint degeneration. Moreover, the co-administration of low doses of CORM-2 or CoPP with DADS or GYY4137 produced higher antiallodynic effects and greater recovery of grip strength deficits than those produced by each of these compounds alone. The activation of the antioxidant system caused by HS donors in DRG and/or PAG might explain the enhancement of antinociceptive effects. These data reveal a positive interaction between HS and CO in modulating joint pain in female mice.
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http://dx.doi.org/10.3390/antiox11071271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312227PMC
June 2022

Cobalt-promoted synthesis of sulfurated oxindoles radical annulation of -arylacrylamides with disulfides.

Org Biomol Chem 2022 Jul 26. Epub 2022 Jul 26.

Guizhou Provincial Engineering Technology Research Center for Chemical Drug R&D, College of Pharmacy, Guizhou Medical University, Guiyang, 550025, China.

An efficient radical annulation of -arylacrylamides with disulfides is developed for the synthesis of sulfurated oxindoles. The reaction occurs in a facile manner using CoBr as both an initiator and a promoter for the first time and (NH)SO as the oxidant. By controlling the CoBr/(NH)SO ratio, a wide range of sulfurated and brominated/sulfurated oxindoles are selectively prepared in good to excellent yields. The present protocol is simple and highly atom economical, and can tolerate a broad range of substrates.
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http://dx.doi.org/10.1039/d2ob00877gDOI Listing
July 2022

Microbiome, Transcriptome, and Metabolomic Analyses Revealed the Mechanism of Immune Response to Diarrhea in Rabbits Fed Antibiotic-Free Diets.

Front Microbiol 2022 6;13:888984. Epub 2022 Jul 6.

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.

In this study, diarrhea was induced in rabbits by feeding them antibiotic-free feed. The gut provides important defense against the barriers of the body, of which the duodenum is an important part to help digest food and absorb nutrients. However, the mechanisms underlying the roles of the gut microbiome and fecal metabolome in rabbit diarrhea caused by feeding an antibiotic-free diet have not been characterized. Recently, only a single study has been conducted to further characterize the antibiotic-free feed additives that caused diarrhea in weaned rabbits. The multi-omics techniques, including 16S rRNA sequencing, transcriptome sequencing, and LC-MS analysis, were combined to analyze the gut microbial compositions and functions. They also determined the fecal metabolomic profiles of diarrhea in rabbits caused by feeding antibiotic-free feed. The results showed that the liver, duodenal, and sacculus rotundus tissues of diarrhea rabbits were diseased, the composition of intestinal microbes was significantly changed, the diversity of intestinal microbes was decreased, and the distribution of intestinal microbe groups was changed. Functional analysis based on the cluster of GO and KEGG annotations suggested that two functional GO categories belonged to the metabolism cluster, and five KEGG pathways related to the metabolic pathways were significantly enriched in diarrhea rabbits. Moreover, real-time quantitative PCR (RT-qPCR) was used to verify the significant expression of genes related to diarrhea. Metabolomics profiling identified 432 significantly differently abundant metabolites in diarrhea rabbits, including amino acids and their derivatives. These amino acids were enriched in the tryptophan metabolic pathway. In addition, the functional correlation analysis showed that some altered gut microbiota families, such as , significantly correlated with alterations in fecal metabolites. Collectively, the results suggested that altered gut microbiota was associated with diarrhea caused by antibiotic-free feed additives in weaned rabbit pathogenesis.
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http://dx.doi.org/10.3389/fmicb.2022.888984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298518PMC
July 2022

Gene Network Mechanism of Zhilong Huoxue Tongyu Capsule in Treating Cerebral Ischemia-Reperfusion.

Front Pharmacol 2022 7;13:912392. Epub 2022 Jul 7.

Liaoning Key Laboratory of Diabetic Cognitive and Perceptive Dysfunction, Jinzhou Medical University, Jinzhou, China.

To investigate the effect of Zhilong Huoxue Tongyu capsule (ZLH) in the treatment of cerebral ischemia-reperfusion injury and determine the underlying molecular network mechanism. The treatment effect of Zhilong Huoxue Tongyu capsule (ZLH) was evaluated for cerebral ischemia-reperfusion injury in middle cerebral artery occlusion (MACO) rat, and the underlying molecular network mechanism was explored by using molecular network analysis based on network pharmacology, bioinformatics including protein-protein interaction (PPI) network, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG), as well as molecular docking. The neurological function of rats in the ZLH group was significantly improved compared to those in the NS group ( = 0.000), confirming the positive effect of ZLH for the treatment of brain ischemia. There were 126 intersecting genes screened in ischemia-reperfusion cerebrum that are associated with several important biological processes, such as lipopolysaccharide, and the most important cell component, such as raft, as well as the most important molecular function pointed as cytokine receptor binding. The most important KEGG signaling pathway was the AGE-RAGE signaling pathway in diabetic complications. Moreover, according to the STRING interaction in the PPI network, 10 hub genes including MAPK14, FOS, MAPK1, JUN, MYC, RELA, ESR1, STAT1, AKT1, and IL6 were selected and exhibited in Cytoscape and molecular docking. Lastly, the relation between PPI, GO, and KEGG was analyzed. These findings indicated that multiple hub network genes have been involved in behavior improvement in cerebral ischemia-reperfusion rats subjected to ZLH treatment. Zhilong Huoxue Tongyu capsule improves cerebral ischemia-reperfusion and is associated with multiple network gene expressions.
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http://dx.doi.org/10.3389/fphar.2022.912392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302771PMC
July 2022

Burnout Among Medical Staff 1 Year After the Beginning of the Major Public Health Emergency in Wuhan, China.

Front Psychol 2022 5;13:893389. Epub 2022 Jul 5.

Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, China.

Objectives: Wuhan is the city where coronavirus disease (COVID-19) was first reported and developed into a pandemic. However, the impact of the prolonged COVID-19 pandemic on medical staff burnout remains limited. We aimed to identify the prevalence and major determinants of burnout among medical staff 1 year after the beginning of the COVID-19 pandemic in Wuhan, China.

Materials And Methods: A total of 1,602 medical staff from three hospitals in Wuhan, China, were included from November 1-28, 2021. Chi-square tests were conducted to compare the prevalence of burnout across groups based on sociodemographic and professional characteristics. A multivariate analysis was performed using a forward stepwise logistic regression model.

Results: Approximately 37.39% of the medical staff experienced burnout 1 year after COVID-19 pandemic. Emotional exhaustion (EE) was the most common symptom of burnout, with 1,422 (88.76%) participants reporting a severe EE. Burnout was associated with insufficient social support and "neutral" or "dissatisfied" patient-physician relationships. Respondents who participated in the care of COVID-19 patients had a higher risk of burnout symptoms than those who did not participate. In particular, mental resilience was negatively associated with burnout among the medical staff.

Conclusion: Nearly two-fifths of the participants had symptoms of burnout, with reduced personal accomplishment being the predominant symptom 1 year after COVID-19. Healthcare organizations should regularly measure and monitor burnout among the medical staff. In addition, creating positive work environments and improving the mental resilience of medical staff may be effective ways to reduce burnout.
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http://dx.doi.org/10.3389/fpsyg.2022.893389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295742PMC
July 2022

MiR-203 is an anti-obese microRNA by targeting apical sodium-dependent bile acid transporter.

iScience 2022 Aug 2;25(8):104708. Epub 2022 Jul 2.

Department of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150081, China.

Obesity is characterized by excessive fat deposition within the body. Bile acids (BA) are important regulators for controlling the absorption of lipid. Here we show that miR-203 exerts weight-loss and lipid-lowering effects by increasing total BA excretion in obese rodents. miR-203 overexpression transgenic mice are resistant to high-fat diet (HFD)-induced obesity and dyslipidemia. Moreover, the knockdown of miR-203 deteriorates metabolic disorders. ASBT plays important role in regulating BA homeostasis and is a direct target of miR-203. In human intestinal epithelial cells, overexpression of miR-203 decreases the cellular uptake of BA by inhibiting ASBT. Furthermore, TCF7L2 is downregulated in obese mice and acts as a transcription factor of miR-203. The ASBT mRNA level was positively correlated with the body mass index (BMI) of population, while the miR-203 level was negatively associated with BMI. Taken together, these data suggest miR-203 could be a new therapeutic BA regulator for obesity and dyslipidemia.
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http://dx.doi.org/10.1016/j.isci.2022.104708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287609PMC
August 2022

Proliferation Marker Ki67 as a Stratification Index of Adjuvant Chemotherapy for Resectable Mucosal Melanoma.

Front Oncol 2022 30;12:895672. Epub 2022 Jun 30.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education, Beijing), Department of Melanoma and Sarcoma, Peking University Cancer Hospital and Institute, Beijing, China.

Background: Adjuvant chemotherapy has been shown to produce a favorable prognosis for patients with resectable mucosal melanoma (MM), resulting in the need for stratification to optimally select patients to benefit from adjuvant therapy. This study analyzed Ki67 as a potential stratification index for adjuvant chemotherapy in resectable MM.

Methods: Patients with resected MM who received subsequent adjuvant therapy in Beijing Cancer Hospital between 2010 and 2018 were retrospectively enrolled and analyzed. Relapse-free survival (RFS) and melanoma-specific survival (MSS) curves were used to perform the survival comparisons across different subgroups.

Results: From Jan 2010 to Dec 2018, 1106 MM patients were screened from a database of 4706 patients and 175 of these patients were finally enrolled. A total of 100 patients received temozolomide (TMZ)-based adjuvant chemotherapy and 75 patients received high-dose interferon-α2b (HDI) adjuvant therapy. Compared with HDI, patients who received TMZ-based adjuvant chemotherapy had significantly superior RFS (21.0 vs. 9.6 months, P = 0.002). For patients with low Ki67 expression (<30%), the two regimens showed no significant difference for impact on RFS (33.9 vs. 22.7 months, P = 0.329). However, for patients with high Ki67 expression (≥30%), TMZ-based adjuvant chemotherapy achieved favorable RFS compared with HDI (18.0 vs. 6.7 months, P < 0.001) and tended to improve MSS compared to HDI (41.4 vs. 25.1 months, P = 0.067).

Conclusion: Compared with HDI, adjuvant chemotherapy may be more relevant for patients with Ki67 ≥ 30%. Ki67 may serve as a potential index to distinguish populations benefiting from adjuvant chemotherapy in resectable MM, and may provide a basis for stratification in the selection of adjuvant regimens.
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http://dx.doi.org/10.3389/fonc.2022.895672DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280123PMC
June 2022

Spatial barcoding-enabled highly multiplexed immunoassay with digital microfluidics.

Biosens Bioelectron 2022 Jul 11;215:114557. Epub 2022 Jul 11.

Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No.457, Zhongshan Road, Shahekou District, Dalian, Liaoning, 116023, China. Electronic address:

Digital microfluidics (DMF), facilitating independent manipulation of microliter samples, provides an ideal platform for immunoassay detection; however, suffering limited multiplexity. To address the need, herein we described a digital microfluidics (DMF) platform that realizes spatial barcoding on the Teflon-coated indium tin oxide (ITO) glass side to fulfill highly multiplexed immunoassay (10+) with low-volume samples (∼4 μL) in parallel, representing the highest multiplexing recorded to date for DMF-actuated immunoassay. Planar-based spatial immobilization of multiple capture antibodies was realized on a Teflon-coated ITO glass side, which was then used as the top plate of the DMF device. Droplets containing analytes, secondary antibodies, and fluorescent signaling reporters with low volume, which were electrically manipulated by our DMF control system, were shuttled sequentially along the working electrodes to complete the immuno-reaction. Evaluation of platform performance with recombinant proteins showed excellent sensitivity and reproducibility. To test the feasibility of our platform in analyzing multiplex biomarkers of the immune response, we used lipopolysaccharide-stimulated macrophages as a model system for protein secretion dynamics studies. As a result, temporal profiling of pro-inflammatory cytokine secretion dynamics was obtained. The spatial barcoding strategy presented here is easy-to-operate to enable a more comprehensive evaluation of protein abundance from biological samples, paving the way for new opportunities to realize multiplexity-associated applications with the DMF platform.
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http://dx.doi.org/10.1016/j.bios.2022.114557DOI Listing
July 2022

Influence of intergenerational relationships on depressive symptoms in ageing Chinese adults in Hong Kong: Mediating effects of sense of loneliness.

BMC Geriatr 2022 07 15;22(1):587. Epub 2022 Jul 15.

Department of Applied Social Sciences, The Hong Kong Polytechnic University, Hong Kong, China.

Background: Mental health of older adults could be positively predicted by harmonious parent-adult children relationships, although the mechanism has not been sufficiently demonstrated. This study employed sense of loneliness as mediator to examine the influence of multiple domains of intergenerational relationships on depressive symptoms in ageing Chinese adults.

Methods: Data was extracted from a representative survey in Hong Kong among Chinese adults aged over 50 with at least one adult child (n = 801). Four key domains (structural-associational, consensual-normative, affectual closeness, and intergenerational conflict) were adopted to measure the intergenerational relationship quality. Depressive symptoms were assessed using the five-item Geriatric Depression Scale. The mediating role of sense of loneliness in the association between intergenerational relationships and depressive symptoms were tested by the PROCESS macro in SPSS.

Results: The influence of overall intergenerational relationship quality and its four subdomains on depressive symptoms were significantly mediated by sense of loneliness. Among the four domains, affectual closeness presented the strongest association with older people's depressive symptoms. The effect of intergenerational conflict on depressive symptoms was completely mediated by sense of loneliness, and the effects of remaining three domains were partially mediated. The domain of consensual-normative solidarity received the lowest rating by Chinese older adults.

Conclusion: The psychological pathway that loneliness links intergenerational relationships and depressive symptoms was supported in this study. With respect to improving intergenerational relationships, enhancing affectual connection between older parents and adult children was essential to prevent mental problems. This study calls for more attention to the protective role of diverse social relationships in improving mental health through multiple pathways.
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http://dx.doi.org/10.1186/s12877-022-03269-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287879PMC
July 2022

Multi-target tracheloside and doxorubicin combined treatment of lung adenocarcinoma.

Biomed Pharmacother 2022 Jul 11;153:113392. Epub 2022 Jul 11.

School of Life Sciences, Hebei University, Baoding 071002, Hebei, China. Electronic address:

Chemotherapy is one of the main methods for malignant lung cancer treatment. However, the side effects of chemotherapy drugs are serious and it is prone to drug resistance. Therefore, multi-drug combination chemotherapy is popular in lung cancer treatment. This study found that tracheloside (TCS) was a novel inhibitor of TMEM16A which was specific high expressed in lung cancer tissues. TCS concentration dependently inhibited TMEM16A with an IC of 3.09 ± 0.21 μM. It inhibited lung cancer cells proliferation, migration, and induced cells apoptosis targeting TMEM16A. In addition, molecular docking combined with site-directed mutagenesis confirmed that the binding sites of TCS to TMEM16A were S387, E623, E624. Subsequently, multi-target combined drug administration was conducted based on the different drug targets of TCS and doxorubicin (DOX). Both in vitro and in vivo experiments indicated that the combined administration of low concentration of TCS and DOX achieved satisfactory anticancer effect, and it offset the side effects caused by high concentration of DOX. Therefore, TCS is a safe and efficient anticancer lead compound which can enhance the effect of DOX.
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http://dx.doi.org/10.1016/j.biopha.2022.113392DOI Listing
July 2022

Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival.

Sci China Life Sci 2022 Jul 7. Epub 2022 Jul 7.

Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China.

Genome-wide association studies have suggested a link between primary open-angle glaucoma and the function of ABCA1. ABCA1 is a key regulator of cholesterol efflux and the biogenesis of high-density lipoprotein (HDL) particles. Here, we showed that the POAG risk allele near ABCA1 attenuated ABCA1 expression in cultured cells. Consistently, POAG patients exhibited lower ABCA1 expression, reduced HDL, and higher cholesterol in white blood cells. Ablation of Abca1 in mice failed to form HDL, leading to elevated cholesterol levels in the retina. Counting retinal ganglion cells (RGCs) by using an artificial intelligence (AI) program revealed that Abca1-deficient mice progressively lost RGCs with age. Single-cell RNA sequencing (scRNA-seq) revealed aberrant oxidative phosphorylation in the Abca1 retina, as well as activation of the mTORC1 signaling pathway and suppression of autophagy. Treatment of Abca1 mice using atorvastatin reduced the cholesterol level in the retina, thereby improving metabolism and protecting RGCs from death. Collectively, we show that lower ABCA1 expression and lower HDL are risk factors for POAG. Accumulated cholesterol in the Abca1 retina causes profound aberrant metabolism, leading to a POAG-like phenotype that can be prevented by atorvastatin. Our findings establish statin use as a preventive treatment for POAG associated with lower ABCA1 expression.
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http://dx.doi.org/10.1007/s11427-021-2126-2DOI Listing
July 2022

Characteristics and Diagnostic Challenge of Antineutrophil Cytoplasmic Antibody Positive Infective Endocarditis.

Am J Med 2022 Jul 9. Epub 2022 Jul 9.

Department of Nephrology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Objective: Antineutrophil cytoplasmic antibody (ANCA) has been reported in patients with infective endocarditis. Whether ANCA is associated with certain characteristics of infective endocarditis is unclear. The principal aim of this study is to investigate the clinical implications of ANCA in infective endocarditis and highlight the diagnostic challenge in ANCA-positive patients with infective endocarditis.

Methods: A retrospective study was conducted in a tertiary hospital in China from August 2012 to December 2021. Patients with a diagnosis of infective endocarditis and available ANCA results were included in the study. The clinical and pathological characteristics were compared between ANCA-positive and ANCA-negative patients.

Results: A total of 237 patients were included. Forty three (18.1%) were ANCA-positive, predominantly c-ANCA/anti-PR3. Compared to ANCA-negative patients, ANCA-positive patients had longer disease duration (P = .004), more frequent purpura (P = .015), macrohematuria (P = .002), proteinuria (P = .043), acute kidney injury (P = .004), and rapidly progressive glomerulonephritis (P = .010). Histologic findings of 8 patients with infective endocarditis-associated glomerulonephritis were reviewed. Two ANCA-positive patients with infective endocarditis presented with pauci-immune necrotizing and crescentic glomerulonephritis. A total of 18.6% of ANCA-positive patients with infective endocarditis were misdiagnosed as ANCA-associated vasculitis.

Conclusions: ANCA is detected in a substantial proportion of patients with infective endocarditis. The presence of ANCA in infective endocarditis is associated with longer disease duration, more frequent purpura, and kidney involvement. ANCA-positive infective endocarditis may mimic ANCA-associated vasculitis, and the differential diagnosis is challenging. Whether ANCA is pathogenic in infective endocarditis-associated small vessel vasculitis requires further study.
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http://dx.doi.org/10.1016/j.amjmed.2022.06.015DOI Listing
July 2022

Monitoring dynamic changes in chicken freshness at 4 °C and 25 °C using pH-sensitive intelligent films based on sodium alginate and purple sweet potato peel extracts.

Int J Biol Macromol 2022 Jul 5;216:361-373. Epub 2022 Jul 5.

College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic address:

A pH-sensitive intelligent indicator film was developed and used for monitoring dynamic changes in chicken freshness at 4 °C and 25 °C by immobilizing 0.2 %-1.0 % purple sweet potato peel extracts (PPE) with sodium alginate (SA). The films presented a wide range of colors from red-pink to green-yellow at 2-13, and the films with less PPE were more sensitive to ammonia. The color of films with 0.6 % PPE changed from pink to blue when used in monitoring chicken freshness at 4 °C (5 d) and 25 °C (60 h), which corresponded to changes in total volatile base nitrogen from 5.35 (5.35) mg/100 g to 16.2 (19.9) mg/100 g. Scanning electron microscopy and X-ray diffraction revealed that PPE improved the compactness and crystallinity of SA films, while Fourier transform infrared spectroscopy revealed hydrogen bonds between SA and PPE. Compared to SA films, the water vapor and light barrier abilities of films with 0.6 % were significantly improved (P < 0.05), there was no significant effect on tensile strength (P > 0.05), and the elongation of 0.6 % PPE films (P < 0.05) was decreased. Thus, PPE can serve as an excellent indicator of intelligent films for monitoring the freshness of meat products.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.06.198DOI Listing
July 2022

Efficient treatment of aged landfill leachate containing high ammonia nitrogen concentration using dynamic wave stripping: Insights into influencing factors and kinetic mechanism.

Waste Manag 2022 Jul 5;150:48-56. Epub 2022 Jul 5.

College of Ecological Environment and Urban Construction, Fujian University of Technology, Fuzhou, Fujian 350118, China. Electronic address:

Aged landfill leachate is challenging to treat owing to its extremely high ammonia concentration and poor biodegradability. We constructed pilot-scale dynamic wave stripping equipment to separate ammonia from landfill leachate and achieved excellent results. To further expand the usage of pilot-scale equipment in actual water treatment process and implement it in a sewage plant, we established the mass transfer kinetic physics and mathematical model of the dynamic wave stripping process based on the surface renewal theory and the traditional stripping method. The surface renewal theory and the traditional stripping method are employed to analyze the mechanism of various experimental parameters affecting the stripping process, predict the stripping effect of the equipment under different conditions, and verify the calculation results of the model using the kinetic fitting results of the experimental data. These calculation results of the model indicate that the mass transfer kinetic coefficients of ammonia stripping at 20 °C, 25 °C, and 30 °C are 85.62 min, 75.34 min, and 65.88 min, respectively, when the gas-liquid ratio is 129. When the gas-liquid ratios are 62, 129, and 163 at 25 °C, the mass transfer kinetic coefficients of ammonia stripping are 102.61 min, 75.34 min, and 61.43 min, respectively. With increasing temperature and gas-liquid ratio, the particle size and number of bubbles in the wave tube of the stripping equipment gradually decrease and the mass transfer efficiency of free ammonia between the gas and liquid phases improves, enhancing the stripping efficiency of ammonia nitrogen.
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http://dx.doi.org/10.1016/j.wasman.2022.06.035DOI Listing
July 2022

Cholinergic dysautonomia in a patient with systemic lupus erythematosus.

Rheumatology (Oxford) 2022 Jul 5. Epub 2022 Jul 5.

Department of Rheumatology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

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http://dx.doi.org/10.1093/rheumatology/keac373DOI Listing
July 2022

The complete chloroplast genome sequence of (Lauraceae): genome structure and phylogenetic analysis.

Mitochondrial DNA B Resour 2022 29;7(6):1194-1195. Epub 2022 Jun 29.

College of Tea Science, Guizhou University, Guiyang, China.

, naturally distributed in southern China, is economic and ecological tree. Its leaves are one of the primary raw materials for processing . Here, we assembled the complete chloroplast genome of using Illumina pair-end sequencing. The chloroplast genome size was 152,739 base pairs (bp) with 39% GC content, containing a pair of inverted repeats (IRA/B) of 20,067 bp, separated by a large- and a small single-copy region (LSC/SSC) of 93,711 bp and 18,894 bp, respectively. The genome encoded 126 genes, including 82 protein-coding, 36 transfer RNA (tRNA), and eight ribosomal RNA (rRNA) genes. Phylogenetic analysis based on chloroplast genome sequences of 22 species suggested that was closely related to species of genus than other genera in Lauraceae. These results can be a valuable genome resource for further genetics and molecular biology studies.
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http://dx.doi.org/10.1080/23802359.2022.2090297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248934PMC
June 2022

Construction of a competency evaluation index system for front-line nurses during the outbreak of major infectious diseases: A Delphi study.

PLoS One 2022 1;17(7):e0270902. Epub 2022 Jul 1.

Department of Endocrinology and Breast Surgery, The First Hospital of Chongqing Medical University, Chongqing, China.

Introduction: As the frequency of infectious diseases rises, it's more important than ever to pay attention to the competency level of front-line nurses as the primary force in front-line rescue, which has an impact on the quality of anti-epidemic response. This paper aims to construct the competency evaluation index system for front-line nurses during the outbreak of major infectious diseases.

Materials And Methods: This study combined literature review, critical incident technique interviews, and semi-structured in-depth interviews, as well as two rounds of Delphi expert correspondence, to construct a competence evaluation index system for front-line nurses during the outbreak of major infectious diseases. The study used purposive sampling to select 26 experts from 11 provinces and cities across China to conduct two rounds of Delphi expert consultation, and the indicators were selected based on the mean importance score > 3.5 and the coefficient of variation < 0.25, and the weights of the indicators were calculated by the Analytic Hierarchy Process. The effective recovery rates of the two rounds of correspondence questionnaires were 93.1% and 96%.

Results: The effective recovery rates of the two rounds of correspondence questionnaires were 93.1% and 96%, the authority coefficients of experts were 0.96 and 0.98, the Kendall's coordination coefficients of the first, second, and third level indexes were 0.281, 0.132, and 0.285 (P < 0.001), 0.259, 0.158, and 0.415 (P < 0.001). The final index system includes 4 primary indicators (Knowledge System of Infectious Diseases, Nursing Skills for Infectious Diseases, Related Professional Abilities for Infectious Diseases, and Comprehensive Quality), 10 secondary indicators, and 64 tertiary indicators.

Conclusion: The competency evaluation index system of front-line nurses during the outbreak of major infectious diseases is scientific, reasonable, and practical, which can provide a scientific basis for nursing managers to accurately understand, describe, analyze, and evaluate the competence level of nursing staff and scientifically implement the allocation of human resources in the future, as well as serve as a content framework for subsequent training programs.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270902PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249240PMC
July 2022
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