Publications by authors named "Xudong Hu"

82 Publications

Effects of consecutive culture of Penaeus vannamei on phosphorus transformation and microbial community in sediment.

Environ Sci Pollut Res Int 2021 Jun 17. Epub 2021 Jun 17.

School of Life Science, Shaoxing University, Shaoxing, Zhejiang, 312000, China.

Phosphorus (P) is highly related to water quality during shrimp culture. Recognizing P transformation in pond-based cultures is crucial for sustainable and healthy aquaculture. However, P transformation remains unclear in the sediment of Penaeus vannamei cultures, although commercial species have been pervasive worldwide. To determine P transformation, samples with different culture years were collected from Zhejiang province, China. Sequential chemical extraction was applied to reveal the composition of inorganic P, while phosphatase activity was used to evaluate the biomineralization of organic P. The results indicated that the consecutive culture of Penaeus vannamei promoted the dissolution potential of sedimentary P. This was attributed to anoxic iron reduction that increased the formation of loosely bound P and Fe (II)-P. However, this phenomenon was dominated by biomineralization, which transformed the organic P to inorganic P. The results suggested that consecutive culture changed the microbial community structure in the sediment as well as the gene functions. The Shannon Wiener index showed that increasing the culture duration significantly decreased the stability of the microbial community. Overall, this study suggests that long-term consecutive culture of Penaeus vannamei may increase the P release potential of the sediment, which increases the risk of pond eutrophication.
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http://dx.doi.org/10.1007/s11356-021-14894-3DOI Listing
June 2021

Three-Dimensional Imaging of Whole-Body Zebrafish Revealed Lipid Disorders Associated with Niemann-Pick Disease Type C1.

Anal Chem 2021 Jun 1;93(23):8178-8187. Epub 2021 Jun 1.

Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China.

Imaging of lipids of whole-body specimens in two-dimensional (2D) analysis provides a global picture of the lipid changes in lipid-disturbed diseases, enabling a better understanding of lipid functions and lipid-modulation processes in different organs. However, 2D imaging of a single cross section can hardly characterize the whole-body lipid alterations. In this work, a three-dimensional matrix-assisted laser desorption/ionization mass spectrometry imaging (3D MALDI-MSI) approach was developed for analysis of whole-body zebrafish, for the first time, and applied to identify altered lipids and map their spatial distributions by using a zebrafish model of Niemann-Pick disease type C1 (NPC1), a neurovisceral lipid storage disorder causing both neurodegenerative disorder and visceral organ damage. The constructed 3D fish model provided comprehensive information on the 3D distribution of lipids of interest and allowed direct correlations between these lipids and organs of the fish. Obtained results revealed that several sphingolipids and phospholipids showed significant alterations and exhibited different localization patterns in various organs such as the brain, spinal cord, intestines, and liver-spleen region in the gene mutant fish compared to those of the wild type. The whole-body 3D MALDI-MSI approach revealed unique lipid signatures for different NPC1-affected organs, which might offer insights into the link between the impaired lipid storage and subsequent clinical symptoms, such as neurodegeneration and hepatosplenomegaly.
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http://dx.doi.org/10.1021/acs.analchem.1c00196DOI Listing
June 2021

Association of SMAD4 loss with drug resistance in clinical cancer patients: A systematic meta-analysis.

PLoS One 2021 28;16(5):e0250634. Epub 2021 May 28.

School of Basic Medical Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: Drug resistance frequently led to the failure of chemotherapy for malignant cancers, hence causing cancer relapse. Thus, understanding mechanism of drug resistance in cancer is vital to improve the treatment efficacy. Here, we aim to evaluate the association between SMAD4 expression and the drug resistance in cancers by performing a meta-analysis.

Method: Relevant studies detecting SMAD4 expression in cancer patients treated with chemo-drugs up till December 2020 were systematically searched in four common scientific databases using selected keywords. The pooled hazard ratio (HR) was the ratio of hazard rate between SMAD4neg population vs SMAD4pos population. The HRs and risk ratios (RRs) with 95% confidence intervals (CIs) were used to explore the association between SMAD4 expression losses with drug resistance in cancers.

Result: After an initial screening according to the inclusion and exclusion criteria, eleven studies were included in the meta-analysis. There were a total of 2092 patients from all the included studies in this analysis. Results obtained indicated that loss of SMAD4 expression was significantly correlated with drug resistance with pooled HRs (95% CI) of 1.23 (1.01-1.45), metastasis with pooled RRs (95% CI) of 1.10 (0.97-1.25) and recurrence with pooled RRs (95% CI) of 1.32 (1.06-1.64). In the subgroup analysis, cancer type, drug type, sample size and antibody brand did not affect the significance of association between loss of SMAD4 expression and drug resistance. In addition, there was no evidence of publication bias as suggested by Begg's test.

Conclusion: Findings from our meta-analysis demonstrated that loss of SMAD4 expression was correlated with drug resistance, metastasis and recurrence. Therefore, SMAD4 expression could be potentially used as a molecular marker for cancer resistance.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250634PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162645PMC
May 2021

Spinal astrocyte aldehyde dehydrogenase-2 mediates ethanol metabolism and analgesia in mice.

Br J Anaesth 2021 Apr 29. Epub 2021 Apr 29.

Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD, USA. Electronic address:

Background: Little is known about the targets in the CNS that mediate ethanol analgesia. This study explores the role of spinal astrocyte aldehyde dehydrogenase-2 (ALDH2), a key ethanol-metabolising enzyme, in the analgesic effects of ethanol in mice.

Methods: Astrocyte and hepatocyte ALHD2-deficient mice were generated and tested in acute and chronic pain models. Cell-type-specific distribution of ALDH2 was analysed by RNA in situ hybridisation in spinal slices from astrocytic ALDH2-deficient mice and their wild-type littermates. Spinal ethanol metabolites and γ-aminobutyric acid (GABA) content were measured using gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry.

Results: ALDH2 mRNA was expressed in both astrocytes and neurones in spinal cord slices. Astrocyte ALDH2-deficient mice had decreased expression of ALDH2 mRNA in astrocytes, but not in neurones. Astrocyte ALDH2 deficiency inhibited ethanol-derived acetate, but not acetaldehyde content in spinal cord tissues. Depletion of spinal astrocyte ALDH2 selectively inhibited ethanol-induced anti-nociceptive effect, but not the effect of ethanol, on motor function. Astrocyte ALDH2 deficiency abolished ethanol-induced GABA elevation. The ethanol metabolite acetate produced anti-nociception and increased GABA synthesis in a manner similar to ethanol. I.T. delivery of either GABA or GABA receptor antagonists prevented ethanol and acetate-induced analgesia.

Conclusions: These findings provide evidence that ALDH2 in spinal astrocytes mediates spinal ethanol metabolism and ethanol-induced analgesic effects by promoting GABA synthesis and GABAergic transmission in spinal cord.
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http://dx.doi.org/10.1016/j.bja.2021.02.035DOI Listing
April 2021

High Child-Pugh and CRUB65 scores predict mortality of decompensated cirrhosis patients with COVID-19: A 23-center, retrospective study.

Virulence 2021 12;12(1):1199-1208

Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.

: COVID-19 has rapidly become a major health emergency worldwide. The characteristic, outcome, and risk factor of COVID-19 in patients with decompensated cirrhosis remain unclear.: Medical records were collected from 23 Chinese hospitals. Patients with decompensated cirrhosis and age- and sex-matched non-liver disease patients were enrolled with 1:4 ratio using stratified sampling.: There were more comorbidities with higher Chalson Complication Index (p < 0.001), higher proportion of patients having gastrointestinal bleeding, jaundice, ascites, and diarrhea among those patients (p < 0.05) and in decompensated cirrhosis patients. Mortality (p < 0.05) and the proportion of severe ill (p < 0.001) were significantly high among those patients. Patients in severe ill subgroup had higher mortality (p < 0.001), MELD, and CRUB65 score but lower lymphocytes count. Besides, this subgroup had larger proportion of patients with abnormal (PT), activated partial thromboplatin time (APTT), D-Dimer, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBL) and Creatinine (Cr) (p < 0.05). Multivariate logistic regression for severity shown that MELD and CRUB65 score reached significance. Higher Child-Pugh and CRUB65 scores were found among non-survival cases and multivariate logistic regression further inferred risk factors for adverse outcome. Receiver Operating Characteristic (ROC) curves also provided remarkable demonstrations for the predictive ability of Child-Pugh and CRUB65 scores.: COVID-19 patients with cirrhosis had larger proportion of more severely disease and higher mortality. MELD and CRUB65 score at hospital admission may predict COVID-19 severity while Child-Pugh and CRUB65 score were highly associated with non-survival among those patients.
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http://dx.doi.org/10.1080/21505594.2021.1909894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078510PMC
December 2021

Occupational Burnout Among Frontline Health Professionals in a High-Risk Area During the COVID-19 Outbreak: A Structural Equation Model.

Front Psychiatry 2021 26;12:575005. Epub 2021 Mar 26.

Department of Psychiatry, Hunan Medical Center for Mental Health, National Clinical Research Center for Mental Disorders, The Second Xiangya Hospital of Central South University, Changsha, China.

The outbreak of coronavirus disease 2019 (COVID-19) resulted in a substantial workload and stress for frontline health professionals in high-risk areas. Little research has investigated the mechanism of occupational burnout among the frontline health professionals located in the center of the epidemic in Wuhan, China. A total of 199 frontline health professionals from Wuhan Jinyintan Hospital completed the cross-sectional survey. Mechanisms of occupational burnout (according to the Maslach Burnout Inventory-General Survey, MBI-GS) among the health professionals in Jinyintan Hospital during the COVID-19 outbreak were examined using a structural equation model (SEM). The levels of the three burnout dimensions (emotional exhaustion, cynicism, and professional efficacy) were high at 34.2, 50.8, and 35.2%, respectively. Frontline health professionals in this stressful period reported significantly greater emotional exhaustion ( < 0.001) and job-related cynicism ( < 0.001), but no significant difference in professional efficacy ( = 0.449), when compared to employees in a large multinational company. The SEM results revealed that both acute stress symptoms and psychosomatic symptoms significantly predicted the emotional exhaustion and occupation cynicism dimensions of burnout. The study reveals the occupational burnout mechanism of frontline health professionals during the COVID-19 peak at the time of the outbreak. This study provides an important contribution to understanding the future psychological interventions necessary for frontline health professionals during an epidemic crisis.
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http://dx.doi.org/10.3389/fpsyt.2021.575005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032859PMC
March 2021

Protein Phosphatase PP1 Negatively Regulates IRF3 in Response to GCRV Infection in Grass Carp ().

Front Immunol 2020 22;11:609890. Epub 2021 Jan 22.

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

Protein phosphatase-1 (PP1) has an important role in many cell functions, such as cell differentiation, development, immune response and tumorigenesis. However, the specific role of PP1 in the antiviral response in fish remains to be elucidated. In this study, the homolog was identified in the grass carp () and its role in defence against the GCRV infection was investigated. Phylogenetic analysis demonstrated that CiPPP1R3G clustered with homologues from other teleosts. Temporal expression analysis revealed that the expression level of was significantly up-regulated in response to GCRV infection in grass carps, especially in the intestine and head-kidney. Cellular distribution analysis revealed that CiPPP1R3G was located in the nucleus and cytoplasm. Overexpression of significantly negatively regulated the expression of CiIRF3, thus inhibiting its activation. In summary, we systematically analyzed the gene in grass carp and illustrated its function as a negative regulator in the anti-GCRV immune responses.
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http://dx.doi.org/10.3389/fimmu.2020.609890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873974PMC
January 2021

Revision surgery after cement augmentation for osteoporotic vertebral fracture.

Orthop Traumatol Surg Res 2020 Dec 15:102796. Epub 2020 Dec 15.

Department of Spine Surgery, Ningbo No.6 Hospital, Zhong Shan East Road 1059#, Ningbo, Zhejiang Province, China. Electronic address:

Introduction: Cement augmentation has been proven to be a safe procedure for the treatment of osteoporotic vertebral fracture, and the overall result is encouraging. However, failures caused by different complications are not uncommon, and a few patients even need revision surgery. This retrospective study aimed to investigate the primary causes of revision surgery after cement augmentation for osteoporotic vertebral fracture and how to prevent them, and the second objective was to evaluate the clinical results of revision surgery.

Hypothesis: The main hypothesis is that infection and neurological dysfunction are the prime causes of revision surgery after cement augmentation for osteoporotic vertebral fracture, and the second hypothesis is that revision surgery is effective to improve the quality of daily life.

Materials And Methods: Twenty-one patients who underwent unplanned revision surgery after cement augmentation were retrospectively analyzed. The initial radiographic and medical records were reviewed to re-evaluate whether the initial diagnosis and surgical procedure were correct and suitable. The primary causes of revision surgery and the details were recorded. The visual analog scale (VAS) and Oswestry Disability Index (ODI) were used to evaluate the efficacy of the revision surgery, and patients with neurological dysfunction were evaluated with the American Spinal Injury Association (ASIA).

Results: A total of 47.6% (10/21) of patients were misdiagnosed initially or had an incorrect indication for cement augmentation surgery, including 3 pre-existing spondylitis cases that were misdiagnosed as osteoporotic vertebral fracture and 7 cases of three column fractures that were treated with cement augmentation. The primary causes and details of the revision surgeries were as follows: 7 patients with infection underwent anterior and/or posterior debridement and fixation; 9 patients with progressive kyphosis underwent posterior osteotomy and fixation; and 5 patients with neurological dysfunction underwent posterior decompression and fixation. The mean follow-up time was 30.6±8.7 months, the VAS score decreased from 7.3±1.1 preoperatively to 2.3±0.3 (p<0.05) at the last follow-up, the ODI decreased from 61.1%±16.3% preoperatively to 20.6%±6.2% (p<0.05) at the last follow-up, and 5 patients with neurological dysfunction improved by at least one grade.

Conclusion: Infection, progressive kyphosis and neurological dysfunction are the primary causes of revision surgery after cement augmentation for osteoporotic vertebral fracture. Avoiding misdiagnosis and ensuring suitable indications might be the key points to decreasing unplanned revision surgery for cement augmentation for osteoporotic vertebral fracture. The quality of daily life and neurological function can be improved through revision surgery.
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http://dx.doi.org/10.1016/j.otsr.2020.102796DOI Listing
December 2020

Infection of human sweat glands by SARS-CoV-2.

Cell Discov 2020 Nov 13;6(1):84. Epub 2020 Nov 13.

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, Hubei 430071, China.

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http://dx.doi.org/10.1038/s41421-020-00229-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661176PMC
November 2020

Discrimination of Tourette Syndrome Based on the Spatial Patterns of the Resting-State EEG Network.

Brain Topogr 2021 01 31;34(1):78-87. Epub 2020 Oct 31.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, No.2006, Xiyuan Ave, West Hi-Tech Zone, Chengdu, 611731, SiChuan, China.

Tourette syndrome (TS) is a neuropsychiatric disorder with childhood onset characterized by chronic motor and vocal tics; however, the current diagnosis of TS patients is subjective, as it is mainly assessed based on the parents' description alongside specific evaluations. The early and accurate diagnosis of TS based on its potential symptoms in children would be of benefit in their future therapy, but reliable diagnoses are difficult due to the lack of objective knowledge of the etiology and pathogenesis of TS. In this study, resting-state electroencephalograms were first collected from 36 patients and 21 healthy controls (HCs); the corresponding resting-state functional networks were then constructed, and the potential differences in network topology between the two groups were extracted by using the topology of the spatial pattern of the network (SPN). Compared to the HCs, the TS patients exhibited decreased frontotemporal/occipital/parietal connectivity. When classifying the two groups, compared to the network properties, the derived SPN features achieved a much higher accuracy of 92.31%. The intrinsic long-range connectivity between the frontal and the temporal/occipital/parietal lobes was damaged in the patient group, and this dysfunctional network pattern might serve as a reliable biomarker to differentiate TS patients from HCs as well as to assess the severity of tic symptoms.
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http://dx.doi.org/10.1007/s10548-020-00801-5DOI Listing
January 2021

Posterior short segment fixation including the fractured vertebra combined with kyphoplasty for unstable thoracolumbar osteoporotic burst fracture.

BMC Musculoskelet Disord 2020 Aug 21;21(1):566. Epub 2020 Aug 21.

Department of Spine Surgery, Ningbo No.6 Hospital, Zhongshan East Road 1059#, Ningbo, 315040, Zhejiang Province, China.

Background: Various studies have described the efficacy and safety of the treatment for unstable thoracolumbar osteoporotic burst fracture, however, there is still no consensus on the optimal treatment regimen. The aim of this study was to evaluate the clinical and radiographic results of posterior short segment fixation including the fractured vertebra (PSFFV) combined with kyphoplasty (KP) for unstable thoracolumbar osteoporotic burst fracture.

Methods: Forty-three patients with unstable thoracolumbar osteoporotic burst fracture underwent PSFFV combined with KP from January 2015 to December 2017 were analyzed retrospectively. Visual Analogue Scale (VAS) and Oswestry Disability Index (ODI) was used to evaluate the clinical outcome, radiological parametres including local kyphotic Cobb angle, percentage of the anterior, middle and posterior height of the fractured vertebra were measured and compared pre-operation, post-operation and at final follow-up.

Results: All patients underwent surgery successfully and with an average follow-up of 19.2 ± 6.7 months (rang 15-32). The VAS decreased from 7.1 ± 2.3 pre-operation to 1.6 ± 0.4 at the final follow-up (p < 0.05). The ODI decreased from 83.1 ± 10.5 pre-operation to 19.2 ± 7.3 (P < 0.05) at the final follow-up. The correction of local kyphotic angle was 16.9° ± 5.3° (p < 0.05), and the loss of correction was 3.3° ± 2.6° (p > 0.05), the correction of anterior vertebral height was 30.8% ± 8.6% (p < 0.05), and the loss of correction was 4.5% ± 3.9% (p > 0.05), the correction of middle vertebral height was 26.4% ± 5.8% (p < 0.05), and the loss of correction was 2.0% ± 1.6% (p > 0.05), the correction of posterior vertebral height was 9.4% ± 6.9% (p < 0.05), and the loss of correction was 1.6% ± 1.3% (p > 0.05). Two cases of screw pullout and 8 cases of cement leakage were observed, but without clinical consequence.

Conclusions: PSFFV combined with KP is a reliable and safe procedure with satisfactory clinical and radiological results for the treatment of unstable thoracolumbar osteoporotic burst fracture.
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http://dx.doi.org/10.1186/s12891-020-03576-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442982PMC
August 2020

Fisch Flavonoids (PCFF) Suppresses the M1 Polarization of LPS-Stimulated RAW264.7 Macrophages by Inhibiting NF-κB/iNOS Signaling Pathway.

Front Pharmacol 2020 18;11:864. Epub 2020 Jun 18.

Cardiovascular Department, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: M1 macrophage plays an important role in inflammatory reaction. In this study, potential anti-inflammatory effect of Fisch flavonoids (PCFF) was assessed Zebrafish acute inflammation model and LPS-induced pro-inflammatory M1 macrophage model .

Methods: The quality control of Fisch flavonoids (PCFF) was analyzed by HPLC. Anti-inflammatory effect of PCFF on the acute injured zebrafish was evaluated by the migration of fluorescence labeled macrophages and neutrophils, and the gene expression of inflammatory factors. In addition, the anti-inflammatory mechanism of PCFF was investigated by the related gene expression and related signaling pathway regulation of pro-inflammatory mediators in LPS-induced pro-inflammatory M1 RAW264.7 macrophage.

Results: Fisch flavonoids (PCFF) markedly suppressed macrophage and neutrophil migration and iNOS gene expression in acute injured zebrafish with tail-cutting. PCFF significantly inhibited NO overproduction and iNOS gene overexpression in LPS-sitimulated pro-inflammatory M1 RAW264.7 macrophages. What's more, PCFF could evidently decrease p65 protein production, but had no effect on the production of P38, JNK and ERK1/2 proteins.

Conclusion: Fisch flavonoids (PCFF) have a remarkable inhibitory effect on the inflammatory response in acute injured zebrafish and LPS-stimulated M1 RAW264.7 macrophage. The pharmacological mechanism may be related to the regulation of NO overproduction and the inhibition of NF-κB/iNOS signaling pathway.
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http://dx.doi.org/10.3389/fphar.2020.00864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314944PMC
June 2020

Histopathological examination and transcriptome analyses to assess the acute toxic effects of arsenite exposure on rare minnows (Gobiocypris rarus).

Ecotoxicology 2020 Jul 8;29(5):613-624. Epub 2020 May 8.

The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.

Arsenic is ubiquitously present in the aquatic environment. We investigated the acute toxic effects of arsenite [As(III)] exposure on rare minnows (Gobiocypris rarus) in vivo. The 96-h LC value for exposure to As(III) was 13.73 mg/L. As(III) bioaccumulation in different tissues was measured using inductively-coupled plasma mass spectrometry, and the extent of As(III) accumulation was, from greatest to least, liver > intestine > gills > muscle > kidney > testis > brain. Histological examination revealed that in As(III)-treated fish, numerous cellular and tissue alterations were present in the gill, liver, and intestine tissues. Moreover, transmission electron microscopy showed ultrastructural alterations in hepatocytes. We also performed transcriptome analyses to investigate As(III)-induced toxicity response in the liver of As(III)-treated fish; various oxidative-related genes were differentially expressed, and their expression levels were further validated using qPCR. This study is one of the many steps we aim to take on the way to promote the rare minnow to an international standard laboratory animal.
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http://dx.doi.org/10.1007/s10646-020-02222-3DOI Listing
July 2020

Acute toxic effects of lead (Pb) exposure to rare minnow (Gobiocypris rarus) revealed by histopathological examination and transcriptome analysis.

Environ Toxicol Pharmacol 2020 Aug 9;78:103385. Epub 2020 Apr 9.

The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address:

Lead (Pb) is a toxic heavy metal that can cause significant damage to the aquatic ecosystem. In this study, acute toxicity test of lead in rare minnow (Gobiocypris rarus) was conducted. The average LC for 96 h of Pb is 423.01 μg/L (95 % CI: 338.41-531.92 μg/L). The order of bioaccumulation of Pb was gills > kidney > intestine > liver > muscle > brain. A number of cellular and tissue alterations were observed in the gill, liver, kidney and intestine tissues of Pb-treated rare minnows through the histological examination performed by H&E and TEM analyses. Furthermore, we investigated the Pb-induced toxicity mechanisms in rare minnow based on transcriptome analyses, and a panel of immune-related genes were identified and evaluated by real-time quantitative PCR. In summary, our work indicates that rare minnow could be a valuable model for studying the mechanisms of lead acute toxicity in fish.
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http://dx.doi.org/10.1016/j.etap.2020.103385DOI Listing
August 2020

Enhanced Peltier Effect in Wrinkled Graphene Constriction by Nano-Bubble Engineering.

Small 2020 Apr 27;16(14):e1907170. Epub 2020 Feb 27.

State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.

Inspired by the promising applications in thermopower generation from waste heat and active on-chip cooling, the thermoelectric and electrothermal properties of graphene have been extensively pursued by seeking ingeniously designed structures with thermoelectric conversion capability. The graphene wrinkle is a ubiquitous structure formed inevitably during the synthesis of large-scale graphene films but the corresponding properties for thermoelectric and electrothermal applications are rarely investigated. Here, the electrothermal Peltier effect from the graphene wrinkle fabricated on a germanium substrate is reported. Peltier cooling and heating across the wrinkle are visualized unambiguously with polarities consistent with p-type doping and in accordance with the wrinkle spatial distribution. By direct patterning of the nano-bubble structure, the current density across the wrinkle can be boosted by current crowding to enhance the Peltier effect. The observed Peltier effect can be attributed to the nonequilibrium charge transport by interlayer tunneling across the van der Waals barrier of the graphene wrinkle. The graphene wrinkle in combination with nano-bubble engineering constitutes an innovative and agile platform to design graphene and other more general two-dimensional (2D) thermoelectrics and opens the possibility for realizing active on-chip cooling for 2D nanoelectronics with van der Waals junctions.
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http://dx.doi.org/10.1002/smll.201907170DOI Listing
April 2020

Use CT Imaging to Predict the Short-Term Outcome of Concurrent Chemoradiotherapy in Patients With Locally Advanced Esophageal Squamous Cell Carcinoma.

Dose Response 2019 Oct-Dec;17(4):1559325819897175. Epub 2019 Dec 30.

Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.

Objective: To extract the computed tomography (CT) imaging features of the primary lesions in patients with advanced esophageal squamous cell carcinoma (ESCC) and to study whether these imaging features can predict the short-term outcome after concurrent chemoradiotherapy (CCRT).

Methods: From January 2014 to December 2015, a total of 49 patients with locally advanced ESCC who underwent CCRT were analyzed retrospectively. They were randomly categorized into the training and validation groups. Collection of CT imaging of patients before and intermediate stage undergoing radiotherapy. The correlations between imaging characteristics and short-term outcome were analyzed. The accuracy of cutoff value was verified by imaging characteristics of patients in validation group.

Result: There were 38 patients in the training group and 11 patients in the validation group. 13 patients in the training group were classified as responders and 25 patients as nonresponders. According to the CT imaging before radiotherapy, there are no significant differences between responders and nonresponders. According to the CT imaging in the middle stage of radiotherapy, responders showed significantly higher Roundness than nonresponders ( = .004, 95% confidence interval [CI] = 0.0419-0.212). The areas under the ROC curves for the ability to predict significantly tumor response were 0.768 for Roundness ( = .001, 95% CI = 0.603-0.889). The cutoff value of Roundness is 0.3099. Roundness showed no significant associations with survival parameters.

Conclusions: Computed tomography imaging in the middle stage of radiotherapy can predict the short-term outcome of concurrent chemoradiotherapy for patients with locally advanced ESCC but have no predictive effect on the total survival time.
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http://dx.doi.org/10.1177/1559325819897175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6937540PMC
December 2019

Comparison Between Extracorporeal Shock Wave Therapy and Local Corticosteroid Injection for Plantar Fasciitis.

Foot Ankle Int 2020 02 19;41(2):200-205. Epub 2019 Nov 19.

Ningbo No. 6 Hospital, Ningbo, China.

Background: Extracorporeal shock wave therapy (ESWT) is a nonsurgical treatment for plantar fasciitis (PF) that has had satisfactory clinical outcomes. However, local corticosteroid injection (LCI) is often regarded as first-line treatment of PF, but there have been few studies comparing the 2 methods. Therefore, we compared the effect of ESWT and LCI on patients with PF.

Methods: This was a block randomized controlled study comparing 49 patients treated with ESWT and 47 patients treated with LCI from January 2017 to December 2018 who were followed for 6 months. We evaluated the clinical outcomes in the 2 groups, including average pain, first-step pain, plantar fascia thickness, and Foot Function Index, Chinese version of the PF patients.

Results: All patients had pain relief and function improvement after treatment, whereas the LCI group did not maintain significant clinical improvement at the 3-month follow-up. The patients in the ESWT group had a significantly better clinical outcome with better duration of improvement than the LCI group.

Conclusion: For PF patients, both ESWT and LCI resulted in clinical improvement but EWST provided longer relief than LCI.

Level Of Evidence: Level II, prospective comparative study.
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http://dx.doi.org/10.1177/1071100719891111DOI Listing
February 2020

Once-weekly bortezomib had similar effectiveness and lower thrombocytopenia occurrence compared with twice-weekly bortezomib regimen in treating patients with newly diagnosed multiple myeloma in China.

Medicine (Baltimore) 2019 Sep;98(39):e17147

Department of Hematology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University.

The study aims to examine the treatment effect and adverse reactions of patients with newly diagnosed MM receiving different bortezomib-based regimens.This was a retrospective study of patients with newly diagnosed MM and who were treated with bortezomib-based combined chemotherapy at the Department of Hematology of the 2 affiliated hospitals of Wenzhou Medical University between July 2009 and May 2016. Cox proportion hazard multivariate analyses were carried out to assess the differences in treatment effect and adverse events between standard (1.3 mg/m on days 1, 4, 8, 11) and weekly (1.6 mg/m on days 1, 8, 15) cohorts, as well as the differences between intravenous injection and subcutaneous injection therapy. Progression-free survival (PFS) and overall survival (OS) were assessed using Kaplan-Meier method and the log-rank test.Among the 117 patients, 78 patients were treated with bortezomib standard therapy and 39 patients were treated with bortezomib weekly therapy (all with intravenous injection). In all patients, the treatment strategy was not independently associated with PFS or OS. The patients in the weekly therapy group had less thrombocytopenia events than those in the standard therapy group. The subcutaneous route had similar treatment effect as the intravenous route, but the incidence of peripheral neuropathy was lower.The once-weekly bortezomib regimen was similar in effectiveness to standard therapy in treating patients with newly diagnosed MM, but the incidence of thrombocytopenia was lower with the weekly regimen compared with the standard regimen.
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http://dx.doi.org/10.1097/MD.0000000000017147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6775427PMC
September 2019

Fabric Moisture Uniform Control to Study the Influence of Air Impingement Parameters on Fabric Drying Characteristics.

J Vis Exp 2019 08 19(150). Epub 2019 Aug 19.

Faculty of Mechanical Engineering & Automation, Zhejiang Sci-Tech University.

Impinging dryness is now a widely used and effective way for fabric drying due to its high heat and mass transfer coefficient. Previous studies on fabric drying have neglected the contributions of moisture uniformity and diffusion coefficient to the drying process; though, they have recently been shown to have a significant influence on drying characteristics. This report outlines a step-by-step procedure to investigate the effects of air impingement parameters on a fabric's drying characteristics by controlling the uniformity of its area moisture distribution. A hot air blower unit equipped with an angle adjustable nozzle is used to generate air flow with different velocities and temperatures while the drying process is recorded and analyzed using an infrared thermograph. In addition, a uniform padder is adapted to ensure the fabric's moisture uniformity. Impinging drying is studied under different initial conditions by changing the air flow temperature, velocity, and direction, then the applicability and suitability of the protocol are evaluated.
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http://dx.doi.org/10.3791/59522DOI Listing
August 2019

Association of Twice-Daily Radiotherapy With Subsequent Brain Metastases in Adults With Small Cell Lung Cancer.

JAMA Netw Open 2019 05 3;2(5):e190103. Epub 2019 May 3.

Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, Shandong, China.

Importance: Although thoracic twice-daily radiotherapy (TDRT) is one of the standards of care for small cell lung cancer, its association with brain metastases remains unknown.

Objective: To investigate the association of TDRT vs once-daily radiotherapy (ODRT) with brain metastases after prophylactic cranial irradiation in patients with small cell lung cancer.

Design, Setting, And Participants: In this multicenter cohort study, data on 778 consecutive patients with small cell lung cancer who had undergone thoracic radiotherapy (609 received ODRT and 169 received TDRT), chemotherapy, and prophylactic cranial irradiation were retrieved from the databases of 8 hospitals in China between July 1, 2003, and June 30, 2016. A 1:1 propensity score matching approach was used to control for confounding between the ODRT and TDRT groups. Confounding covariates included 8 demographic variables and 8 treatment-related covariates. Data analysis was conducted from November 1, 2017, to May 31, 2018, and reanalyzed for revision.

Exposures: The ODRT group received 50 to 66 Gy given in 25 to 33 fractions. The TDRT group received 45 Gy given in 30 fractions.

Main Outcomes And Measures: The primary end point was brain metastases. Secondary end points included progression-free survival and overall survival.

Results: Of the 778 patients (median age, 55 years [interquartile range, 48-61 years]), 204 were women and 574 were men. At a median follow-up of 23.6 months (interquartile range, 14.2-38.2 months), 131 patients (16.8%) experienced brain metastases. The rate of brain metastasis at 3 years in the TDRT group was significantly higher than in the ODRT group (26.0% vs 16.9%; hazard ratio, 1.55; 95% CI, 1.06-2.26; P = .03). Of the 338 matched patients (169 in the ODRT group vs 169 in the TDRT group), 60 (17.8%) experienced brain metastases, with a rate at 3 years of 14.9% in the ODRT group vs 26.0% in the TDRT group (hazard ratio, 1.71; 95% CI, 1.02-2.88; P = .04). Progression-free survival was similar in both the whole cohort and the matched cohort. Median overall survival in the ODRT group tended to be significantly longer than in the TDRT group after matching (47.2 vs 32.8 months; hazard ratio, 1.41; 95% CI, 0.99-2.01; P = .06).

Conclusions And Relevance: In this study, patients with small cell lung cancer who received thoracic TDRT appeared to have a higher risk of brain metastases than those who received ODRT, which supports the need for further prospective randomized clinical trials, especially in China and other parts of Asia.
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http://dx.doi.org/10.1001/jamanetworkopen.2019.0103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6537825PMC
May 2019

Role of feline sarcoma‑related protein in the viability and apoptosis of bladder cancer cells.

Mol Med Rep 2019 Jun 30;19(6):5219-5226. Epub 2019 Apr 30.

Department of Nutrition and Food Safety, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China.

Feline sarcoma‑related protein (Fer) is a type of nuclear and cytoplasmic non‑receptor protein tyrosine kinase, which is associated with the progression of numerous types of cancer. Previously, we identified that Fer is associated with the migration and invasion of bladder cancer. The present study aimed to investigate the role of Fer in bladder cancer cell viability and apoptosis. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were performed to detect the expression levels of Fer; short interference RNA (siRNA) and overexpression vectors were used to downregulate or upregulate Fer expression, respectively. The effects on cell proliferation ability and cell apoptosis were then tested by MTT assay and flow cytometry. The results revealed that Fer expression was upregulated in bladder cancer cell lines. Downregulation of Fer expression by siRNA significantly suppressed T24 cell viability and induced apoptosis, as well as inducing cell cycle arrest. Conversely, Fer overexpression in 5637 cells significantly promoted cell viability and cell cycle progression, but inhibited cell apoptosis. Furthermore, the suppression and overexpression of Fer significantly altered the expression of cleaved caspase‑3 and Bcl‑2, and dysregulated the P38 mitogen‑activated protein kinase signaling pathway. The findings of the present study indicate a possible molecular mechanism of Fer in bladder cancer and may be considered as a potential target in the treatment of this disease.
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http://dx.doi.org/10.3892/mmr.2019.10204DOI Listing
June 2019

Diagnostic and Predictive Value of Using RGD PET/CT in Patients with Cancer: A Systematic Review and Meta-Analysis.

Biomed Res Int 2019 10;2019:8534761. Epub 2019 Jan 10.

Department of Radiation Oncology, Shandong Cancer Hospital and Institute-Shandong Cancer Hospital Affiliated to Shandong University, No. 440 Jiyan Road, Jinan 250117, Shandong, China.

The purpose of this study was to assess the diagnostic value of arginine-glycine-aspartic acid (RGD) PET/CT for tumor detection in patients with suspected malignant lesions and to determine the predictive performance of RGD PET/CT in identifying responders. . The PubMed (Medline), EMBASE, Cochrane Library, and Web of Science databases were systematically searched for potentially relevant publications (last updated on July 28th, 2018) reporting the performance of RGD PET in the field of oncology. Pooled sensitivities, specificities, and diagnostic odds ratios (DORs) were calculated for parameters. The areas under the curve (AUCs) and Q⁎ index scores were determined from the constructed summary receiver operating characteristic (SROC) curve. We explored heterogeneity by metaregression. . Nine studies, five including 216 patients that determined diagnostic performance and three including 75 patients that determined the predictive value of parameters, met our inclusion criteria. The pooled sensitivity, pooled specificity, DOR, AUC, and Q⁎ index score of RGD PET/CT for the detection of underlying malignancy were 0.85 (0.79-0.89), 0.93 (0.90-0.96), 48.35 (18.95-123.33), 0.9262 (standard error=0.0216), and 0.8606 for SUVmax and 0.86 (0.80-0.91), 0.92 (0.88-0.94), 40.49 (14.16-115.77), 0.9312 (SE=0.0177), and 0.8665 for SUVmean, respectively. The pooled sensitivity, pooled specificity, DOR, AUC, and Q⁎ index score of RGD PET/CT for identifying responders were 0.80 (0.59-0.93), 0.74 (0.60-0.85), 15.76 (4.33-57.32), 0.8682 (0.0539), and 0.7988, respectively, for SUVmax at baseline. . The interesting but preliminary data in this meta-analysis demonstrate that RGD PET/CT may be an ideal diagnostic tool for detecting underlying malignancies in patients suspected of having tumors and may be able to efficiently predict short-term outcomes.
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http://dx.doi.org/10.1155/2019/8534761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348803PMC
May 2019

Formation of Si Hollow Structures as Promising Anode Materials through Reduction of Silica in AlCl-NaCl Molten Salt.

ACS Nano 2018 Nov 5;12(11):11481-11490. Epub 2018 Nov 5.

Shanghai Key Laboratory of Special Artificial Microstructure Materials and Technology, School of Physics Science and Engineering , Tongji University , Shanghai 200092 , China.

Hollow nanostructures are attractive for energy storage and conversion, drug delivery, and catalysis applications. Although these hollow nanostructures of compounds can be generated through the processes involving the well-established Kirkendall effect or ion exchange method, a similar process for the synthesis of the pure-substance one ( e. g., Si) remains elusive. Inspired by the above two methods, we introduce a continuous ultrathin carbon layer on the silica nano/microstructures (Stöber spheres, diatom frustules, sphere in sphere) as the stable reaction interface. With the layer as the diffusion mediator of the reactants, silica structures are successfully reduced into their porous silicon hollow counterparts with metal Al powder in AlCl-NaCl molten salt. The structures are composed of silicon nanocrystallites with sizes of 15-25 nm. The formation mechanism can be explained as an etching-reduction/nucleation-growth process. When used as the anode material, the silicon hollow structure from diatom frustules delivers specific capacities of 2179, 1988, 1798, 1505, 1240, and 974 mA h g at 0.5, 1, 2, 4, 6, and 8 A g, respectively. After being prelithiated, it retains 80% of the initial capacity after 1100 cycles at 8 A g. This work provides a general way to synthesize versatile silicon hollow structures for high-performance lithium ion batteries due to the existence of ample silica reactants and can be extended to the synthesis of hollow structures of other materials.
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http://dx.doi.org/10.1021/acsnano.8b06528DOI Listing
November 2018

Pretreatment PET/CT imaging of angiogenesis based on F-RGD tracer uptake may predict antiangiogenic response.

Eur J Nucl Med Mol Imaging 2019 Apr 5;46(4):940-947. Epub 2018 Sep 5.

Department of Radiation Oncology, Shandong Cancer Hospital and Institute-Shandong Cancer Hospital Affiliated to Shandong University, No 440 Jiyan Road, Jinan, 250117, Shandong, China.

Purpose: To explore the relationship between metabolic uptake of the F-ALF-NOTA-PRGD (F-RGD) tracer on positron emission tomography/computerized tomography (PET/CT) and the antiangiogenic effect of apatinib in patients with solid malignancies.

Materials And Patients: Patients with measurable lesions scheduled for second- or third-line single-agent therapy with apatinib were eligible for this prospective clinical trial. All patients underwent F-RGD PET/CT examination before the start of treatment. Standardized uptake values (SUVs) of contoured tumor lesions were computed and compared using independent sample t-tests or the Mann-Whitney U test. Receiver-operating characteristic (ROC) curve analysis was used to determine accuracy in predicting response. Survival curves were compared using the Kaplan-Meier method.

Results: Of 38 patients who consented to study participation, 25 patients with 42 measurable lesions met the criteria for inclusion in this response assessment analysis. The median follow-up time was 3 months (range, 1-10 months), and the median progression-free survival (PFS) was 3 months (95% confidence interval, 1.04-4.96). The SUV and SUV were significantly higher in responding tumors than in non-responding tumors (4.98 ± 2.34 vs 3.59 ± 1.44, p = 0.048; 3.71 ± 1.15 vs 2.95 ± 0.49, P = 0.036). SUV did not differ between responding tumors and non-responding tumors (6.58 ± 3.33 vs 4.74 ± 1.83, P = 0.078). An exploratory ROC curve analysis indicated that SUV [area under the ROC curve (AUC) = 0.700] was a better parameter than SUV (AUC = 0.689) for predicting response. Using a threshold value of 3.82, high SUV at baseline was associated with improved PFS (5.0 vs. 3.4 months, log-rank P = 0.036).

Conclusion: F-RGD uptake on PET/CT imaging pretreatment may predict the response to antiangiogenic therapy, with higher F-RGD uptake in tumors predicting a better response to apatinib therapy.
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http://dx.doi.org/10.1007/s00259-018-4143-8DOI Listing
April 2019

A Simple One-Pot Strategy for Synthesizing Ultrafine SnS Nanoparticle/Graphene Composites as Anodes for Lithium/Sodium-Ion Batteries.

ChemSusChem 2018 May 17;11(9):1549-1557. Epub 2018 Apr 17.

School of Materials Science and Engineering, Key Laboratory of Advanced Ceramics and Machining Technology of Ministry of Education, Tianjin University, Tianjin, 300072, P. R. China.

SnS /graphene composites have attracted extensive attention in energy storage owing to their excellent electrochemical performance. However, most of the previous methods to synthesize SnS /graphene composites require long times, high temperatures, or high pressures, which are obstacles for practical low-cost production. A simple one-pot strategy to prepare SnS /graphene composites has been developed, which is not time-consuming (1 h) and requires moderate temperature (75 °C) in atmosphere. Through this method, ultrafine SnS nanoparticles anchored on graphene nanosheets are prepared and exhibit excellent electrochemical performance for both lithium and sodium storage. Specifically, as anodes for lithium-ion batteries, the SnS /graphene electrode delivers a high capacity of 1480 mAh g after 50 cycles at 0.2 A g . Even at 10 A g , the SnS /graphene electrode can achieve a capacity of 666 mAh g . A constructed full lithium-ion cell exhibits a capacity of 957 mAh g after 50 cycles at 1 A g . This simple one-pot strategy may pave the way for large-scale production and practical application of SnS /graphene composites in energy storage.
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http://dx.doi.org/10.1002/cssc.201800073DOI Listing
May 2018

Highly reversible and fast sodium storage boosted by improved interfacial and surface charge transfer derived from the synergistic effect of heterostructures and pseudocapacitance in SnO-based anodes.

Nanoscale 2018 Feb;10(5):2301-2309

School of Materials Science and Engineering, Key Laboratory of Advanced Ceramics and Machining Technology of Ministry of Education, Tianjin University, Tianjin 300072, PR China.

Sodium-ion batteries have attracted worldwide attention as potential alternatives for large scale stationary energy storage due to the rich reserves and low cost of sodium resources. However, the practical application of sodium-ion batteries is restricted by unsatisfying capacity and poor rate capability. Herein, a novel mechanism of improving both interfacial and surface charge transfer is proposed by fabricating a graphene oxide/SnO/CoO nanocomposite through a simple hydrothermal method. The formation of heterostructures between ultrafine SnO and CoO could enhance the charge transfer of interfaces owing to the internal electric field. The pseudocapacitive effect, which is led by the high specific area and the existence of ultrafine nanoparticles, takes on a feature of fast faradaic surface charge-transfer. Benefiting from the synergistic advantages of the heterostructures and the pseudocapacitive effect, the as-prepared graphene oxide/SnO/CoO anode achieved a high reversible capacity of 461 mA h g after 80 cycles at a current density of 0.1 A g. Additionally, at a high current density of 1 A g, a high reversible capacity of 241 mA h g after 500 cycles is obtained. A full cell coupled by the as-prepared graphene oxide/SnO/CoO anode and the NaV(PO) cathode was also constructed, which exhibited a reversible capacity of 310.3 mA h g after 100 cycles at a current density of 1 A g. This method of improving both interfacial and surface charge transfer may pave the way for the development of high performance sodium-ion batteries.
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http://dx.doi.org/10.1039/c7nr07533bDOI Listing
February 2018

Combined neutrophil-platelet score and hemoglobin level predict survival in esophageal squamous cell carcinoma patients treated with chemoradiotherapy.

Oncotarget 2017 Oct 27;8(50):87971-87979. Epub 2017 Sep 27.

Department of Radiotherapy, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, Shandong Province, P.R. China.

Systemic inflammation and hematological markers have prognostic value in patients with esophageal squamous cell carcinoma (ESCC). The objective of this study was to evaluate the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), combined neutrophil-platelet (CNP) score, and hemoglobin (Hb) to inform treatment decisions and predict outcomes in patients with locally advanced ESCC treated with chemoradiotherapy (CRT). A total of 168 patients with locally advanced ESCC were retrospectively evaluated. Patients were stratified by marker value using a receiver operating characteristic curve analysis to determine the cutoff point. Logistic regression was used to identify markers associated with sensitivity to treatment. Overall survival (OS) was calculated by the Kaplan-Meier method. Multivariate Cox logistic regression modeling was used to assess the influences of OS. Smoking history, tumour site, clinical stage, NLR, PLR, CNP, and Hb ( ≤ 0.05) were associated with the sensitivity to therapy. In multivariate analysis, a high CNP score was independently associated with poor treatment sensitivity (OR = 2.066, = 0.021). Univariate analysis revealed that PLR, CNP, and Hb levels were associated with OS, and Cox multivariate analysis found that CNP (HR = 1.47, = 0.027) and Hb level (HR = 0.44, = 0.007) were independent predictors of OS. In conclusion, CNP and Hb are inexpensive and universally available prognostic markers in patients with locally advanced ESCC patients. CNP score is a systemic inflammatory marker that predicted sensitivity to CRT.
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http://dx.doi.org/10.18632/oncotarget.21313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5675686PMC
October 2017

Dosimetric comparison of CT-guided iodine-125 seed stereotactic brachytherapy and stereotactic body radiation therapy in the treatment of NSCLC.

PLoS One 2017 9;12(11):e0187390. Epub 2017 Nov 9.

Cancer Therapy and Research Center, Shandong Provincial Hospital affiliated to Shandong University, Jinan, P. R. China.

This study aimed to assess the dosimetric differences between iodine-125 seed stereotactic brachytherapy (SBT) and stereotactic body radiation therapy (SBRT) in the treatment of non-small cell lung cancer (NSCLC). An SBT plan and an SBRT plan were generated for eleven patients with T1-2 NSCLC. Prescription of the dose and fractionation (fr) for SBRT was 48Gy/4fr. The planning aim for SBT was D90 (dose delivered to 90% of the target volume)≥120Gy. Student's paired t test was used to compare the dosimetric parameters. The SBT and SBRT plans had comparable PTV D90 (104.73±2.10Gyvs.107.64±2.29Gy), and similar mean volume receiving 100% of the prescription dose (V100%) (91.65% vs.92.44%, p = 0.410). The mean volume receiving 150% of the prescribed dose (V150%) for SBT was 64.71%, whereas it was 0% for SBRT. Mean heterogeneity index (HI) deviation for SBT vs. SBRT was 0.73 vs. 0.19 (p<0.0001), and the mean conformity index (CI) for SBT vs. SBRT was 0.77 vs. 0.81 (p = 0.031). The mean lung doses (MLD) in SBT were significantly lower than those in SBRT (1.952±0.713 vs. 5.618±2.009, p<0.0001). In conclusion, compared with SBRT, SBT can generate a comparable dose within PTV, while the organs at risk (OARs) only receive a very low dose. But the HI and CI in SBT were lower than in SBRT.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0187390PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679513PMC
November 2017

A novel molecular agent for glioma angiogenesis imaging.

Nucl Med Commun 2017 Nov;38(11):919-926

aSchool of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences Departments of bRadiation Oncology cPET/CT dPathology, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, Shandong, China.

Background: Gliomas are rich in blood vessels and are the most primary and malignant type of brain tumor affecting the central nervous system. A few fluorine-18 (F)-labeled imaging agents can be used for imaging of tumor angiogenesis. In the current study, F-labeled recombinant human endostatin (rh-endostatin) was developed and evaluated as a probe for PET imaging of tumor angiogenesis.

Materials And Methods: F-fluorobenzoyl-endostatin (F-FB-endostatin) was synthesized from radiolabeling of rh-endostatin with N-succinimidyl-4-F-fluorobenzoate produced by a facile module-assisted radiosynthesis procedure. Blocking studies were used to measure the relative affinities of F-FB-endostatin to human glioblastoma U87MG cells in tumor tissues rich with vessels. In addition, biodistribution, metabolic stability, and small-animal PET imaging studies were carried out with F-FB-endostatin using Institute of Cancer Research and U87MG tumor-bearing mice.

Results: Noninvasive small-animal PET imaging indicated that F-FB-endostatin showed rapid and good tumor uptake. The probe was rapidly cleared from the blood and most organs, resulting in excellent tumor-to-normal tissue contrasts. Tumor uptake and rapid clearance were further confirmed with biodistribution studies. Metabolite assays showed that the probe was highly stable, making it suitable for in-vivo applications.

Conclusion: F-FB-endostatin shows promising in-vivo properties. Therefore, the promising properties of F-FB-endostatin indicate that this probe can be a powerful tool to evaluate the antiangiogenic therapy for gliomas and thus warrants further investigation as a novel PET probe for imaging of tumor angiogenesis.
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http://dx.doi.org/10.1097/MNM.0000000000000735DOI Listing
November 2017