Publications by authors named "Xuan Wang"

1,690 Publications

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Self-polishing antifouling coatings based on benzamide derivatives containing capsaicin.

Mar Pollut Bull 2022 Jun 21;181:113844. Epub 2022 Jun 21.

Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Open Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266100, China; Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, China. Electronic address:

In this study, N-hydroxymethylbenzamide was alkylated with various aromatic compounds to obtain five novel benzamide derivatives containing capsaicin (BDCC), and the BDCC were incorporated into coatings as auxiliary agents. The relationships between properties and structures were discussed based on experimental and theoretical results. The theoretical results showed the optimized configurations of BDCC and confirmed that the benzene ring, phenolic hydroxyl, ester and amide groups were active sites. Experimental results indicated that the antimicrobial and antifouling effects of compounds b1, b2 and b3 were better than those of chlorothalonil, their MIC and MBC values were no more than 64 and 512 μg·mL, and their test panels were covered only with small amounts of dirt and biofilms; they worked well as green antifouling additives. The experimental and theoretical results showed that BDCC and BDCC antifouling coatings were effective and eco-friendly.
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http://dx.doi.org/10.1016/j.marpolbul.2022.113844DOI Listing
June 2022

Association of Dietary Inflammatory Potential with Blood Inflammation: The Prospective Markers on Mild Cognitive Impairment.

Nutrients 2022 Jun 10;14(12). Epub 2022 Jun 10.

Beijing Key Laboratory of Environmental Toxicology, School of Public Health, Capital Medical University, Beijing 100069, China.

Inflammation is known as an important mechanism of cognitive dysfunction. Systemic immune inflammation index (SII) and system inflammation response index (SIRI) are two blood inflammatory markers, which are related to many chronic diseases including cognitive impairment. It is recognized that dietary inflammatory index (DII), which is used to estimate the overall inflammatory potential of diet, may be related to mild cognitive impairment (MCI) as well. This study aimed to explore the relationship between SII, SIRI and DII, as well as the role of these inflammatory indexes on MCI in elderly people. A total of 1050 participants from Beijing were included. Neuropsychological tests were used for cognitive evaluation. Energy-adjusted DII scores were calculated based on semi-quantitative food frequency questionnaire. Blood samples were tested for calculating SII and SIRI. Log-binomial regression models were used to estimate the correlation of indexes. After adjusting demographic characteristics, SII and SIRI in MCI individuals were higher than controls ( ≤ 0.001). DII, SII and SIRI had positive relationship with MoCA scores ( < 0.005). DII also correlated with SIRI in MCI (β = 0.11, = 0.031). Higher DII and SIRI could definitely increase the risk of MCI, as well as DII and SII ( < 0.005). In conclusion, DII was positively correlated with blood inflammation. The elderly with higher level of DII and SIRI, or DII and SII could be considered as people with higher risk of developing MCI.
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http://dx.doi.org/10.3390/nu14122417DOI Listing
June 2022

Changes in laboratory value improvement and mortality rates over the course of the pandemic: an international retrospective cohort study of hospitalised patients infected with SARS-CoV-2.

BMJ Open 2022 Jun 23;12(6):e057725. Epub 2022 Jun 23.

Department of Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA.

Objective: To assess changes in international mortality rates and laboratory recovery rates during hospitalisation for patients hospitalised with SARS-CoV-2 between the first wave (1 March to 30 June 2020) and the second wave (1 July 2020 to 31 January 2021) of the COVID-19 pandemic.

Design, Setting And Participants: This is a retrospective cohort study of 83 178 hospitalised patients admitted between 7 days before or 14 days after PCR-confirmed SARS-CoV-2 infection within the Consortium for Clinical Characterization of COVID-19 by Electronic Health Record, an international multihealthcare system collaborative of 288 hospitals in the USA and Europe. The laboratory recovery rates and mortality rates over time were compared between the two waves of the pandemic.

Primary And Secondary Outcome Measures: The primary outcome was all-cause mortality rate within 28 days after hospitalisation stratified by predicted low, medium and high mortality risk at baseline. The secondary outcome was the average rate of change in laboratory values during the first week of hospitalisation.

Results: Baseline Charlson Comorbidity Index and laboratory values at admission were not significantly different between the first and second waves. The improvement in laboratory values over time was faster in the second wave compared with the first. The average C reactive protein rate of change was -4.72 mg/dL vs -4.14 mg/dL per day (p=0.05). The mortality rates within each risk category significantly decreased over time, with the most substantial decrease in the high-risk group (42.3% in March-April 2020 vs 30.8% in November 2020 to January 2021, p<0.001) and a moderate decrease in the intermediate-risk group (21.5% in March-April 2020 vs 14.3% in November 2020 to January 2021, p<0.001).

Conclusions: Admission profiles of patients hospitalised with SARS-CoV-2 infection did not differ greatly between the first and second waves of the pandemic, but there were notable differences in laboratory improvement rates during hospitalisation. Mortality risks among patients with similar risk profiles decreased over the course of the pandemic. The improvement in laboratory values and mortality risk was consistent across multiple countries.
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http://dx.doi.org/10.1136/bmjopen-2021-057725DOI Listing
June 2022

Correction to "Synthetic Studies toward DNA-Encoded Heterocycles Based on the On-DNA Formation of α, β-Unsaturated Ketones".

Org Lett 2022 Jun 23. Epub 2022 Jun 23.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Zhang Jiang Hi-Tech Park, Pudong, Shanghai 201203, China.

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http://dx.doi.org/10.1021/acs.orglett.2c01936DOI Listing
June 2022

Predictive value of neutrophil-to-lymphocyte ratio for distant metastasis in gastric cancer patients.

Sci Rep 2022 Jun 17;12(1):10269. Epub 2022 Jun 17.

Department of Surgical Oncology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, Shaanxi, China.

As a systemic inflammatory marker, the significance of NLR in predicting tumor prognosis and early lymph node metastasis is well known, including gastric cancer (GC). However, whether NLR can reflect GC metastasis status remains to be explored. We retrospectively enrolled 1667 GC patients treated in our hospital from December 2010 to December 2018. Patients were grouped according to the presence or absence of metastases. Receiver operating characteristics (ROC) curve analysis was used to evaluate the diagnostic efficacy of markers in assessing GC metastasis. Then we conducted a joint ROC curve analysis. The effects of clinicopathological parameters on GC metastasis were assessed using multiple logistic regression analysis. 743 (44.6%) patients were diagnosed with metastatic GC. Patients with GC metastases have younger age, higher CEA, CA19-9, CA72-4 and NLR. Based on the comparison of AUC, NLR has diagnostic efficacy comparable to that of GC markers. The AUC of NLR combined with GC markers had significantly higher predicting efficacy than that without combination for assessing peritoneal metastasis (P = 0.013), osseous metastasis (P = 0.017) and hepatic metastasis (P < 0.001). In multiple logistic regression analysis, age, NLR, CEA, CA19-9 and CA72-4 were found to be independently associated with GC metastasis (all P < 0.05). NLR was a risk factor of GC metastasis. Combining CEA, CA19-9, CA72-4 and NLR could better predict metastases in GC.
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http://dx.doi.org/10.1038/s41598-022-14379-4DOI Listing
June 2022

Naringenin in Si-Ni-San formula inhibits chronic psychological stress-induced breast cancer growth and metastasis by modulating estrogen metabolism through FXR/EST pathway.

J Adv Res 2022 Jun 16. Epub 2022 Jun 16.

Integrative Research Laboratory of Breast Cancer, Discipline of Integrated Chinese and Western Medicine, the Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China; State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China; Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangdong Provincial Academy of Chinese Medical Sciences, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, Guangdong, China; The Research Center for Integrative Medicine, School of basic medical sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China; Guangdong-Hong Kong-Macau Joint Lab on Chinese Medicine and Immune Disease Research, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China. Electronic address:

Intro duction Chronic psychological stress is a well-established risk factor for breast cancer development. Si-Ni-San (SNS) is a classical traditional Chinese medicine formula prescribed to psychological disorder patients. However, its action effects, molecular mechanisms, and bioactive phytochemicals against breast cancer are not yet clear.

Objectives: This study aimed to explore the modulatory mechanism and bioactive compound of SNS in regulating estrogen metabolism during breast cancer development induced by chronic psychological stress.

Methods: Mouse breast cancer xenograft was used to determine the effect of SNS on breast cancer growth and metastasis. Metabolomics analysis was conducted to discover the impact of SNS on metabolic profile changes in vivo. Multiple molecular biology experiments and breast cancer xenografts were applied to verify the anti-metastatic potentials of the screened bioactive compound.

Results: SNS remarkably inhibited chronic psychological stress-induced breast cancer growth and metastasis in the mouse breast cancer xenograft. Meanwhile, chronic psychological stress increased the level of cholic acid, accompanied by the elevation of estradiol. Mechanistic investigation demonstrated that cholic acid activated farnesoid X receptor (FXR) expression, which inhibited hepatocyte nuclear factor 4α (HNF4α)-mediated estrogen sulfotransferase (EST) transcription in hepatocytes, and finally resulting in estradiol elevation. Notably, SNS inhibited breast cancer growth by suppressing estradiol level via modulating FXR/EST signaling. Furthermore, luciferase-reporting gene assay screened naringenin as the most bioactive compound in SNS for triggering EST activity in hepatocytes. Interestingly, pharmacokinetic study revealed that naringenin had the highest absorption in the liver tissue. Following in vivo and in vitro studies demonstrated that naringenin inhibited stress-induced breast cancer growth and metastasis by promoting estradiol metabolism via FXR/EST signaling.

Conclusion: This study not only highlights FXR/EST signaling as a crucial target in mediating stress-induced breast cancer development, but also provides naringenin as a potential candidate for breast cancer endocrine therapy via promoting estradiol metabolism.
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http://dx.doi.org/10.1016/j.jare.2022.06.006DOI Listing
June 2022

A Response to: Letter to the Editor Regarding [Esophageal Dysfunction and Systemic Sclerosis: Drugs Should be Kept in Mind].

Rheumatol Ther 2022 Jun 18. Epub 2022 Jun 18.

Department of Rheumatology and Immunology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, 200065, China.

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http://dx.doi.org/10.1007/s40744-022-00458-5DOI Listing
June 2022

Optimization of initial dose regimen of tacrolimus in paediatric lung transplant recipients based on Monte Carlo simulation.

J Clin Pharm Ther 2022 Jun 18. Epub 2022 Jun 18.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy & School of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.

What Is Known And Objectives: The initial tacrolimus dose regimen in paediatric lung transplant recipients is unknown. The present study optimized the initial tacrolimus dose regimen for paediatric lung transplant recipients.

Methods: This study was based on a published population pharmacokinetic model of tacrolimus in lung transplant recipients and used Monte Carlo simulations to recommend an initial dose regimen of tacrolimus in paediatric lung transplant recipients.

Results: Without voriconazole, the tacrolimus doses recommended for paediatric lung transplant recipients who were not CYP3A5*1 carriers were 0.02, 0.03, and 0.04 mg/kg/day, split into two doses, for weights of 10-16, 16-30, and 30-40 kg, respectively. For paediatric lung transplant recipients who were CYP3A5*1 carriers, the tacrolimus doses of 0.03, 0.04, 0.05, and 0.06 mg/kg/day, split into two doses, were recommended for weights of 10-16, 16-25, 25-30, and 30-40 kg, respectively. With voriconazole, the tacrolimus dose recommended for paediatric lung transplant recipients who were not CYP3A5*1 carriers was 0.02 mg/kg/day, split into two doses, for weights of 10-40 kg. For paediatric lung transplant recipients who were CYP3A5*1 carriers, tacrolimus doses of 0.02 and 0.03 mg/kg/day, split and two doses, were recommended for weights of 10-24 and 24-40 kg, respectively.

What Is New And Conclusions: This study developed tacrolimus dose regimens for the first time for paediatric lung transplant recipients using Monte Carlo simulation and optimized initial dosage in paediatric lung transplant recipients.
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http://dx.doi.org/10.1111/jcpt.13717DOI Listing
June 2022

Effects of contrast enhancement boost postprocessing technique in combination with different reconstruction algorithms on the image quality of abdominal CT angiography.

Eur J Radiol 2022 Jun 2;154:110388. Epub 2022 Jun 2.

Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objectives: To investigate whether contrast-enhancement-boost (CE-boost) in combination with hybrid iterative reconstruction (Hybrid IR, also named HIR [AIDR 3D, adaptive iterative dose reduction three dimensional]) and model-based iterative reconstruction (MBIR [FIRST, forward projected model-based IR solution]) algorithms can improve the image quality of abdominal CT angiography (CTA).

Methods: This retrospective study included 50 patients who underwent abdominal CTA. Both arterial and portal phases were reconstructed using three different algorithms [filtered-back projection (FBP), AIDR 3D, and FIRST] separately. CE-boost was performed additionally to generate AIDR 3D-boost and FIRST-boost images. Signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) of the arteries and portal system were compared among the five datasets (FBP, AIDR 3D, FIRST, AIDR 3D-boost, FIRST-boost). In subjective analyses, two radiologists independently ordered images (5, best; 1, worst) based on the visual image quality of distal arterial or portal venous branches. The Friedman and the Dunn-Bonferroni post-hoc tests were used for statistical analysis.

Results: FIRST-boost arterial and portal images had the lowest noise compared with FBP, AIDR 3D, FIRST, and AIDR 3D-boost images (all P < 0.05), and significantly higher SNR and CNR than FBP, AIDR 3D, and FIRST images (all P < 0.05). AIDR 3D-boost images showed lower noise, and higher SNR and CNR than FBP and AIDR 3D images (all P < 0.05). FIRST-boost images had higher subjective grading scores than FBP, AIDR 3D, and AIDR 3D-boost images (all P < 0.05).

Conclusion: The postprocessing technique CE-boost can improve the image quality of abdominal CTA images. MBIR in combination with CE-boost (FIRST-boost) images had the best image quality compared with the other four image datasets.
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http://dx.doi.org/10.1016/j.ejrad.2022.110388DOI Listing
June 2022

Generation of a human embryonic stem cell line (SMUDHe010-A-82) carrying a homozygous c.1538G > A (p.G513D) mutation in the OSMR gene by CRISPR/Cas9-mediated homologous recombination.

Stem Cell Res 2022 Jun 11;63:102842. Epub 2022 Jun 11.

Department of Dermatology, Dermatology Hospital, Southern Medical University, Guangzhou 510091, China; Joint Laboratory of Dermatology Hospital, Southern Medical University and China Resources Sanjiu Medical & Pharmaceutical Co., Ltd, Guangzhou 510091, China. Electronic address:

Mutations in the tumor suppressor M receptor (OSMR) gene are associated with primary localized cutaneous amyloidosis (PLCA). Recently, we confirmed that OSMR loss-of-function mutations enhance epidermal keratinocyte differentiation via inactivation of the STAT5/KLF7 signaling. However, no disease model was available for PLCA. Accordingly, we generated an OSMR c.1538G > A mutant human embryonic stem cell line (SMUDHe010-A-82) using CRISPR/Cas9-mediated homologous recombination. The cell line preserves normal karyotype, pluripotency and the ability to differentiate into all three germ layers. Moreover, the cell line can be used to prepare human skin organoid, which may provide a disease model for PLCA.
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http://dx.doi.org/10.1016/j.scr.2022.102842DOI Listing
June 2022

Exploration of Different Hypoxia Patterns and Construction of a Hypoxia-Related Gene Prognostic Index in Colorectal Cancer.

Front Immunol 2022 30;13:853352. Epub 2022 May 30.

Department of Radiotherapy, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Introduction: Immune checkpoint inhibitor (ICI) therapy has been proven to be a highly efficacious treatment for colorectal adenocarcinoma (COAD). However, it is still unclear how to identify those who might benefit the most from ICI therapy. Hypoxia facilitates the progression of the tumor from different aspects, including proliferation, metabolism, angiogenesis, and migration, and improves resistance to ICI. Therefore, it is essential to conduct a comprehensive understanding of the influences of hypoxia in COAD and identify a biomarker for predicting the benefit of ICI.

Methods: An unsupervised consensus clustering algorithm was used to identify distinct hypoxia-related patterns for COAD patients from TCGA and the GEO cohorts. The ssGSEA algorithm was then used to explore the different biological processes, KEGG pathways, and immune characteristics among distinct hypoxia-related clusters. Some hypoxia-related hub genes were then selected by weighted gene coexpression network analysis (WGCNA). Subsequently, univariate Cox regression analysis, multivariate Cox regression analysis, and least absolute shrinkage and selection operator (LASSO) regression were utilized to construct a hypoxia-related gene prognostic index (HRGPI). Finally, validation was also conducted for HRGPI in prognostic value, distinguishing hypoxia-related characteristics and benefits of ICI.

Results: We identified four hypoxia-related clusters and found that different hypoxia response patterns induced different prognoses significantly. Again, we found different hypoxia response patterns presented distinct characteristics of biological processes, signaling pathways, and immune features. Severe hypoxia conditions promoted activation of some cancer-related signaling pathways, including Wnt, Notch, ECM-related pathways, and remodeled the tumor microenvironment of COAD, tending to present as an immune-excluded phenotype. Subsequently, we selected nine genes (ANO1, HOXC6, SLC2A4, VIP, CD1A, STC2, OLFM2, ATP6V1B1, HMCN2) to construct our HRGPI, which has shown an excellent prognostic value. Finally, we found that HRGPI has an advantage in distinguishing immune and molecular characteristics of hypoxia response patterns, and it could also be an excellent predictive indicator for clinical response to ICI therapy.

Conclusion: Different hypoxia response patterns activate different signaling pathways, presenting distinct biological processes and immune features. HRGPI is an independent prognostic factor for COAD patients, and it could also be used as an excellent predictive indicator for clinical response to ICI therapy.
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http://dx.doi.org/10.3389/fimmu.2022.853352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196334PMC
June 2022

Improved polar-code-based efficient post-processing algorithm for quantum key distribution.

Sci Rep 2022 Jun 16;12(1):10155. Epub 2022 Jun 16.

School of Computer Science and Technology, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, China.

Combined with one-time pad encryption scheme, quantum key distribution guarantees the unconditional security of communication in theory. However, error correction and privacy amplification in the post-processing phase of quantum key distribution result in high time delay, which limits the final secret key generation rate and the practicability of quantum key distribution systems. To alleviate this limitation, this paper proposes an efficient post-processing algorithm based on polar codes for quantum key distribution. In this algorithm, by analyzing the channel capacity of the main channel and the wiretap channel respectively under the Wyner's wiretap channel model, we design a codeword structure of polar codes, so that the error correction and privacy amplification could be completed synchronously in a single step. Through combining error correction and privacy amplification into one single step, this efficient post-processing algorithm reduces complexity of the system and lower the post-processing delay. Besides, the reliable and secure communicaiton conditions for this algorithm has been given in this paper. Simulation results show that this post-processing algorithm satisfies the reliable and secure communication conditions well.
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http://dx.doi.org/10.1038/s41598-022-14145-6DOI Listing
June 2022

Classification of multi-lead ECG with deep residual convolutional neural networks.

Physiol Meas 2022 Jun 15. Epub 2022 Jun 15.

Fudan University School of Basic Medical Sciences, 138 Yixueyuan Road, Xuhui District, Shanghai, 200032, CHINA.

Objective: Automatic ECG interpretation based on deep learning methods is attracting increasing attention. In this study, we propose a novel method to accurately classify multi-lead ECGs using deep residual neural networks.

Approach: ECG recordings from seven different open databases were provided by PhysioNet/Computing in Cardiology Challenge 2021. All the ECGs were pre-processed to obtain the same sampling rate. The label inconsistency among the databases was corrected by adding or removing specific labels. A label mask was created to filter out potentially incorrectly labelled data. Five models based on deep residual convolutional neural networks were optimized using an asymmetric loss function to classify multi-lead ECGs.

Main Results: The proposed method achieved an official challenge score of 0.54, 0.52, 0.50, 0.51, and 0.50 on twelve-lead, six-lead, four-lead, three-lead, and two-lead ECG test sets, respectively. These scores were ranked 5th, 3rd, 7th, 5th and 7th, respectively, in the challenge.

Significance: The proposed method can correct the differential labeling tendency of databases from different sources and exhibits good generalization for classifying multi-lead ECGs in the hidden test set. The proposed models have the potential for clinical applications.
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http://dx.doi.org/10.1088/1361-6579/ac7939DOI Listing
June 2022

Simulating the effect of different lighting conditions on left-turn driving behavior using a scenario-based anger method.

Sci Rep 2022 Jun 13;12(1):9791. Epub 2022 Jun 13.

School of Business, Sichuan Normal University, Chengdu, China.

Anger is a key factor affecting drivers' subjective judgment and driving skills. The influence of anger on driving behavior has been widely studied, but there is a lack of comparative research under different lighting conditions. Through a driving simulation experiment, this paper studies the influence of anger on left-turn driving behavior under two light conditions day and night. In the experiment, 32 licensed participants were divided into two groups, one in emotional neutrality and the other in anger. Among them, the emotional state of anger is induced by a traffic-related video. The results showed that compared with daytime participants, participants at night had higher anger intensity, shorter gap acceptance, and post encroachment time (PET) when left-turn driving. In addition, compared with neutral emotion participants, angry participants tended to accept shorter gap acceptance and PET when turning left. This indicates that participants failed to respond correctly to left-turn driving behavior in a state of anger. However, the response of gender differences to situational driving anger was not affected by light conditions. The anger intensity of male participants during the day and night was higher than that of female participants, and the gap between acceptance and PETs during left-turn was shorter than that of female participants. This shows that male participants are more likely to produce high-intensity anger and are more likely to make dangerous driving decisions in a state of anger. This paper puts forward some suggestions on identifying anger and preventing angry driving.
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http://dx.doi.org/10.1038/s41598-022-13932-5DOI Listing
June 2022

International comparisons of laboratory values from the 4CE collaborative to predict COVID-19 mortality.

NPJ Digit Med 2022 Jun 13;5(1):74. Epub 2022 Jun 13.

VA Informatics and Computing Infrastructure, VA Salt Lake City Health Care System, Salt Lake City, USA.

Given the growing number of prediction algorithms developed to predict COVID-19 mortality, we evaluated the transportability of a mortality prediction algorithm using a multi-national network of healthcare systems. We predicted COVID-19 mortality using baseline commonly measured laboratory values and standard demographic and clinical covariates across healthcare systems, countries, and continents. Specifically, we trained a Cox regression model with nine measured laboratory test values, standard demographics at admission, and comorbidity burden pre-admission. These models were compared at site, country, and continent level. Of the 39,969 hospitalized patients with COVID-19 (68.6% male), 5717 (14.3%) died. In the Cox model, age, albumin, AST, creatine, CRP, and white blood cell count are most predictive of mortality. The baseline covariates are more predictive of mortality during the early days of COVID-19 hospitalization. Models trained at healthcare systems with larger cohort size largely retain good transportability performance when porting to different sites. The combination of routine laboratory test values at admission along with basic demographic features can predict mortality in patients hospitalized with COVID-19. Importantly, this potentially deployable model differs from prior work by demonstrating not only consistent performance but also reliable transportability across healthcare systems in the US and Europe, highlighting the generalizability of this model and the overall approach.
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http://dx.doi.org/10.1038/s41746-022-00601-0DOI Listing
June 2022

A novel TCF-aza-BODIPY-based near-infrared fluorescent probe for highly selective detection of hypochlorous acid in living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Oct 7;279:121490. Epub 2022 Jun 7.

School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, PR China. Electronic address:

Hypochlorous acid/hypochlorite (HOCl/ClO) plays important roles in killing bacterial and causing damage to living tissues, and its abnormal levels could lead to many diseases. Although great efforts have been devoted, fluorescent probes for HOCl/ClO with near-infrared fluorescence, good selectivity/sensitivity, and low background are still important and urgent. In this work, a novel double-bond-linked TCF-aza-BODIPY-based near-infrared fluorescent probe (3) was rationally designed, successfully prepared, and applied for sensing HOCl/ClO in both solutions and living RAW264.7 cells, showing good selectivity and fluorescence "turn-on" phenomenon at 670 nm with low background. The limit of detection towards ClO was determined to be 0.36 μM through the linear fluorescence changes at 670 nm in a broad ClO-concentration range of 0-150 μM. Furthermore, the sensing mechanism was investigated by mass spectrometry and compared with 1, suggesting that the remarkable spectroscopic changes could be ascribed to the oxidization of the double bond to the aldehyde group, accompanied with the leaving of the TCF group. Confocal imaging experiments also confirmed the remarkable intracellular fluorescence enhancements through incubation of ClO and phorbol ester 12-myristate 13-acetate (PMA) in RAW264.7 cells. Therefore, for the first time, we reported a near-infrared TCF-aza-BODIPY-based fluorescent probe for highly sensitive and fluorescence "turn-on" detection of both exogenous and endogenous HOCl in living RAW264.7 cells through the quick oxidation of a conjugated double bond.
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http://dx.doi.org/10.1016/j.saa.2022.121490DOI Listing
October 2022

Prevalence of inflammatory bowel disease in patients with primary sclerosing cholangitis: A systematic review and meta-analysis.

Liver Int 2022 Jun 11. Epub 2022 Jun 11.

State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.

Background And Aim: Previous studies have established an association between primary sclerosing cholangitis (PSC) and inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis (UC). The disease burden of IBD in PSC patients was not well estimated. The study aimed to quantify the pooled prevalence of IBD in PSC and to investigate whether subtypes of PSC and sex influence the prevalence of IBD.

Methods: PubMed, Embase, and Web of Science were searched through November 2021 for studies reporting data on IBD among PSC patients. The outcomes were the prevalence of IBD in patients with PSC, as well as the association (odds ratio [OR]) of IBD in PSC according to subtype and sex.

Results: Based on the analysis of 25 studies, the prevalence of IBD in patients with PSC was 71.1% (95% CI 68.2-75.1%), most commonly in UC (55.9%, 95% CI 52.5-59.3%). The pooled prevalence of IBD was 76.9% in Australia (95% CI 71.2-82.6%, 1 study), 75.9% (95% CI 69.5-82.3%, 4 studies) in North America, 70.9% (95% CI 65.8-76.0%, 17 studies) in Europe and 67.0% (95% CI 57.9-76.0%, 2 studies) in Asia. Male PSC patients had a higher prevalence of IBD (OR 1.67, 95% CI 1.52-1.83) and UC (OR 2.02, 95% CI 1.56-2.63) and a lower prevalence of CD (OR 0.77, 95% CI 0.67-0.88) than female patients. Large duct PSC patients had a higher prevalence of IBD (OR 2.57, 95% CI 2.03-3.25) and UC (OR 4.51, 95% CI 1.22-16.71) than small duct PSC patients.

Conclusions: The study provided the first pooled estimates of the burden of IBD in patients with PSC and could be used as the basis for risk stratification of PSC patients.
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http://dx.doi.org/10.1111/liv.15339DOI Listing
June 2022

Fabrication and Characterization of a Self-Powered n-BiSe/p-Si Nanowire Bulk Heterojunction Broadband Photodetector.

Nanomaterials (Basel) 2022 May 26;12(11). Epub 2022 May 26.

Henan Province Key Laboratory of Photovoltaic Materials, Henan University, Kaifeng 475004, China.

In the present study, vacuum evaporation method is used to deposit BiSe film onto Si nanowires (NWs) to form bulk heterojunction for the first time. Its photodetector is self-powered, its detection wavelength ranges from 390 nm to 1700 nm and its responsivity reaches its highest value of 84.3 mA/W at 390 nm. In comparison to other BiSe/Si photodetectors previously reported, its infrared detection length is the second longest and its response speed is the third fastest. Before the fabrication of the photodetector, we optimized the growth parameter of the BiSe film and the best BiSe film with atomic steps could finally be achieved. The electrical property measurement conducted by the physical property measurement system (PPMS) showed that the grown BiSe film was n-type conductive and had unique topological insulator properties, such as a metallic state, weak anti-localization (WAL) and linear magnetic resistance (LMR). Subsequently, we fabricated Si NWs by the metal-assisted chemical etching (MACE) method. The interspace between Si NWs and the height of Si NWs could be tuned by Ag deposition and chemical etching times, respectively. Finally, Si NWs fabricated with the Ag deposition time of 60 s and the etching time of 10 min was covered by the best BiSe film to be processed for the photodetector. The primary n-BiSe/p-Si NWs photodetector that we fabricated can work in a self-powered mode and it has a broadband detection range and fast response speed, which indicates that it can serve as a promising silicon-based near- and mid-infrared photodetector.
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http://dx.doi.org/10.3390/nano12111824DOI Listing
May 2022

DNA Methylation of Imprinted Genes , , and in Peripheral Blood Is Associated with the Risk of Breast Cancer.

Cancers (Basel) 2022 May 27;14(11). Epub 2022 May 27.

Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin 150081, China.

Methylation alterations of imprinted genes lead to loss of imprinting (LOI). Although studies have explored the mechanism of LOI in breast cancer (BC) development, the association between imprinted gene methylation in peripheral blood and BC risk is largely unknown. We utilized HumanMethylation450 data from TCGA and GEO ( = 1461) to identify the CpG sites of imprinted genes associated with BC risk. Furthermore, we conducted an independent case-control study ( = 1048) to validate DNA methylation of these CpG sites in peripheral blood and BC susceptibility. cg26709929, cg08446215, cg25306939, and cg16057921, which are located at , , and , were discovered to be associated with BC risk. Subsequently, the association between cg26709929, cg26057921, and cg25306939 methylation and BC risk was validated in our inhouse dataset. All 22 CpG sites in the region were associated with BC risk. Individuals with a hypermethylated region (>0.474) had a lower BC risk (OR: 0.553, 95% CI: 0.397-0.769). Additionally, the methylation of the region was not significantly different among B cells, monocytes, and T cells, which was also observed at CpG sites in . In summary, the methylation of , , and was associated with BC risk, and methylation could be a potential biomarker for BC risk assessment.
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http://dx.doi.org/10.3390/cancers14112652DOI Listing
May 2022

Biochemical recurrence related metabolic novel signature associates with immunity and ADT treatment responses in prostate cancer.

Cancer Med 2022 Jun 9. Epub 2022 Jun 9.

Department of Urology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

Background: Prostate cancer (PCa) is a unique cancer from a metabolic perspective. Androgen receptor assumes a vital part in normal and malignant prostate cells regarding almost all aspects of cell metabolism, such as glucose, fat, amino acids, nucleotides, and so on.

Methods: We used The Cancer Genome Atlas database as training set, Memorial Sloan-Kettering Cancer Center cohort as validation set, and Gene Expression Omnibus database (GSE70769) as test set to identify the optimal prognostic signature. We evaluated the signature in terms of biochemical progression-free survival (bPFS), ROC curve, clinicopathological features, independent prognostic indicators, tumor microenvironment, and infiltrating immune cells. Nomogram was built dependent on the results of cox regression analyses. GSEA algorithm was used to evaluate differences in metabolism. The signature's prediction of androgen deprivation therapy (ADT) response was validated based on two groups of basic cytological experiments treat with ADT (GSE143408 and GSE120343) and the transcriptional information of pre-ADT/post-ADT of six local PCa patients.

Results: We finally input four screened genes into the stepwise regression model to construct metabolism-related signature. The signature shows good prediction performance in training set, verification set, and test set. A nomogram based on the PSA, Gleason score, T staging, and the signature risk score could predict 1-, 3-, and 5-year bPFS with the high area under curve values. Based on gene-set enrichment analysis, the characteristics of four genes signature could influence some important metabolic biological processes of PCa and were serendipitously found to be significantly related to androgen response. Subsequently, two cytological experimental data sets and our local patient sequencing data set verified that the signature may be helpful to evaluate the therapeutic response of PCa to ADT.

Conclusions: Our systematic study definite a metabolism-related gene signature to foresee prognosis of PCa patients which might add to individual prevention and treatment.
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http://dx.doi.org/10.1002/cam4.4856DOI Listing
June 2022

Niobium-doped layered cathode material for high-power and low-temperature sodium-ion batteries.

Nat Commun 2022 Jun 9;13(1):3205. Epub 2022 Jun 9.

Institute for Sustainable Energy/College of Sciences, Shanghai University, Shanghai, 200444, P. R. China.

The application of sodium-based batteries in grid-scale energy storage requires electrode materials that facilitate fast and stable charge storage at various temperatures. However, this goal is not entirely achievable in the case of P2-type layered transition-metal oxides because of the sluggish kinetics and unfavorable electrode|electrolyte interphase formation. To circumvent these issues, we propose a P2-type NaNiMnNbO (P2-NaMNNb) cathode active material where the niobium doping enables reduction in the electronic band gap and ionic diffusion energy barrier while favoring the Na-ion mobility. Via physicochemical characterizations and theoretical calculations, we demonstrate that the niobium induces atomic scale surface reorganization, hindering metal dissolution from the cathode into the electrolyte. We also report the testing of the cathode material in coin cell configuration using Na metal or hard carbon as anode active materials and ether-based electrolyte solutions. Interestingly, the Na||P2-NaMNNb cell can be cycled up to 9.2 A g (50 C), showing a discharge capacity of approximately 65 mAh g at 25 °C. Furthermore, the Na||P2-NaMNNb cell can also be charged/discharged for 1800 cycles at 368 mA g and -40 °C, demonstrating a capacity retention of approximately 76% and a final discharge capacity of approximately 70 mAh g.
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http://dx.doi.org/10.1038/s41467-022-30942-zDOI Listing
June 2022

Dose-dependent immunomodulatory effects of metformin on human neonatal monocyte-derived macrophages.

Cell Immunol 2022 Jul 3;377:104557. Epub 2022 Jun 3.

Laboratory of Neonatology, Department of Neonatology, Affiliated Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, China. Electronic address:

While the association of inflammation with bronchopulmonary dysplasia (BPD) has long been appreciated, M1 proinflammatory macrophage population is emerging as the key element in driving the BPD inflammatory environment. Previous study suggests that low-dose metformin elicits an anti-inflammatory response, possibly through modulating macrophages, to improve disease outcome in a rat BPD model. To investigate this concept further, we examined the dose-dependent immunomodulatory function of metformin directly on human macrophages derived from cord blood (CB) monocytes. We demonstrate that low-dose metformin promotes expansion of M2 anti-inflammatory macrophages, contrasted with high-dose treatment, which exacerbates inflammation by favoring M1 polarization and restricting M2 phenotype. These findings highlight that metformin hold immunomodulatory ability by regulating macrophage polarization in a dose-dependent manner, and only when applied at low dose, exhibiting potential for beneficial anti-inflammatory adjuvant in BPD setting.
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http://dx.doi.org/10.1016/j.cellimm.2022.104557DOI Listing
July 2022

Load Asymmetry Angle Estimation Using Multiple view Videos.

IEEE Trans Hum Mach Syst 2021 Dec;51(6):734-739

University of Wisconsin-Madison, Wisconsin, USA.

A robust computer vision-based approach is developed to estimate the load asymmetry angle defined in the revised NIOSH lifting equation (RNLE). The angle of asymmetry enables the computation of a recommended weight limit for repetitive lifting operations in a workplace to prevent lower back injuries. An open-source package OpenPose is applied to estimate the 2D locations of skeletal joints of the worker from two synchronous videos. Combining these joint location estimates, a computer vision correspondence and depth estimation method is developed to estimate the 3D coordinates of skeletal joints during lifting. The angle of asymmetry is then deduced from a subset of these 3D positions. Error analysis reveals unreliable angle estimates due to occlusions of upper limbs. A robust angle estimation method that mitigates this challenge is developed. We propose a method to flag unreliable angle estimates based on the average confidence level of 2D joint estimates provided by OpenPose. An optimal threshold is derived that balances the percentage variance reduction of the estimation error and the percentage of angle estimates flagged. Tested with 360 lifting instances in a NIOSH-provided dataset, the standard deviation of angle estimation error is reduced from 10.13° to 4.99°. To realize this error variance reduction, 34% of estimated angles are flagged and require further validation.
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http://dx.doi.org/10.1109/thms.2021.3112962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170187PMC
December 2021

Application of intraoperative indocyanine green angiography in the harvest of chimeric deep circumflex iliac artery perforator flaps: a technical note.

Br J Oral Maxillofac Surg 2022 Apr 22. Epub 2022 Apr 22.

Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, 324 Jingwu Road, Jinan 250021, China. Electronic address:

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http://dx.doi.org/10.1016/j.bjoms.2022.03.012DOI Listing
April 2022

Rapid regulations of metabolic reactions in Escherichia coli via light-responsive enzyme redistribution.

Biotechnol J 2022 Jun 4:e2200129. Epub 2022 Jun 4.

School of Life Sciences, Tsinghua University, Beijing, China.

Protein-based condensates have been proposed to accelerate biochemical reactions by enriching reactants and enzymes simultaneously. Here, we engineered those condensates into a photo-activated switch in Escherichia coli (PhASE) to regulate enzymatic reactions via tuning the spatial correlation of enzymes and substrates. In this system, scaffold proteins undergo liquid-liquid phase separation (LLPS) to form light-responsive compartments. Tethered with a light-responsive protein, enzymes of interest (EOIs) can be recruited by those compartments from cytosol within only a few seconds after a pulse of light induction and fully released in 15 min. Furthermore, we managed to enrich small molecular substrates simultaneously with enzymes in the compartments and achieved the acceleration of luciferin and catechol oxidation by 2.3- and 1.6-folds, respectively. We also developed a quantitative model to guide the further optimization of this demixed regulatory system. Our tool can thus be used to study the rapid redistribution of proteins, and reversibly regulate enzymatic reactions in E. coli.
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http://dx.doi.org/10.1002/biot.202200129DOI Listing
June 2022

Revealing the mechanism of Jiegeng decoction attenuates bleomycin-induced pulmonary fibrosis via PI3K/Akt signaling pathway based on lipidomics and transcriptomics.

Phytomedicine 2022 Jul 25;102:154207. Epub 2022 May 25.

Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, China. Electronic address:

Background: Pulmonary fibrosis (PF) is a serious lung disease with unknown etiology and irreversible course. Jiegeng decoction (JGD), a traditional prescription, is widely used to treat lung diseases due to its anti-inflammatory and expectorant effects.

Purpose: To explore the effect of JGD on mice with PF and its underlying mechanism. For this purpose, we established a mouse model with PF by bleomycin (BLM) and then administered JGD and pirfenidone at different concentrations.

Results: In vivo, JGD was found to reduce lung inflammation, improve lung function and decrease collagen deposition to alleviate bleomycin-induced PF in mice. The mouse lung tissue was analyzed using lipidomics and transcriptomics. We found phosphatidylinositol was decreased after JGD treatment in lipidomics results, while transcriptomics results showed the critical roles of PI3K/Akt signaling pathway in JGD treatment group. Then, Western Blot and Immunohistochemistry were used to validate that JGD may regulate the expression of Bax, Caspase3, Caspase8, Caspase9 and Bcl-2 apoptosis-related proteins via PI3K/Akt signaling pathway. TUNEL staining revealed that apoptosis mainly occurs on AEC IIs.

Conclusion: Our results showed that JGD inhibits apoptosis through the PI3K/Akt signaling pathway, thereby protecting against BLM-induced PF. Hence, JGD is expected to be a potential drug candidate for the treatment of PF.
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http://dx.doi.org/10.1016/j.phymed.2022.154207DOI Listing
July 2022

Changes in Circulating miR-375-3p and Improvements in Visceral and Hepatic Fat Contents in Response to Lifestyle Interventions: The CENTRAL Trial.

Diabetes Care 2022 Jun 3. Epub 2022 Jun 3.

Department of Epidemiology, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA.

Objective: To investigate whether changes in circulating levels of pancreatic islet-related miRNA-375 (miR-375) are related to improved visceral and intrahepatic fat accumulation.

Research Design And Methods: This study included adults with abdominal obesity from an 18-month weight loss lifestyle intervention trial. Circulating miR-375-3p was measured at baseline and 18 months. MRI was performed (n = 139) to assess 18-month changes in abdominal and intrahepatic fat depots.

Results: Circulating miR-375-3p was related to fasting insulin and insulin resistance in participants with prediabetes. After the interventions, there was a significant increase of miR-375-3p (P < 0.001). Greater increase in miR-375-3p was associated with greater reductions of visceral (P = 0.024) and deep subcutaneous (P < 0.001) adipose tissues and intrahepatic fat content (P = 0.012).

Conclusions: Increases in circulating miR-375-3p were associated with visceral and intrahepatic fat reduction. Changes in circulating pancreatic islet-related miR-375-3p may be linked to improved diabetogenic fat depots during weight loss lifestyle interventions.
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http://dx.doi.org/10.2337/dc21-2517DOI Listing
June 2022

A Temporal PROTAC Cocktail-Mediated Sequential Degradation of AURKA Abrogates Acute Myeloid Leukemia Stem Cells.

Adv Sci (Weinh) 2022 Jun 2:e2104823. Epub 2022 Jun 2.

Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, China.

AURKA is a potential kinase target in various malignancies. The kinase-independent oncogenic functions partially disclose the inadequate efficacy of the kinase inhibitor in a Phase III clinical trial. Simultaneously targeting the catalytic and noncatalytic functions of AURKA may be a feasible approach. Here, a set of AURKA proteolysis targeting chimeras (PROTACs) are developed. The CRBN-based dAurA383 preferentially degrades the highly abundant mitotic AURKA, while cIAP-based dAurA450 degrades the lowly abundant interphase AURKA in acute myeloid leukemia (AML) cells. The proteomic and transcriptomic analyses indicate that dAurA383 triggers the "mitotic cell cycle" and "stem cell" processes, while dAurA450 inhibits the "MYC/E2F targets" and "stem cell" processes. dAurA383 and dAurA450 are combined as a PROTAC cocktail. The cocktail effectively degrades AURKA, relieves the hook effect, and synergistically inhibits AML stem cells. Furthermore, the PROTAC cocktail induces AML regression in a xenograft mouse model and primary patient blasts. These findings establish the PROTAC cocktail as a promising spatial-temporal drug administration strategy to sequentially eliminate the multifaceted functions of oncoproteins, relieve the hook effect, and prevent cancer stem cell-mediated drug resistance.
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http://dx.doi.org/10.1002/advs.202104823DOI Listing
June 2022

Efficient multicycle terahertz pulse generation based on the tilted pulse-front technique.

Opt Lett 2022 Jun;47(11):2678-2681

Controlling the time-domain oscillation of a terahertz (THz) wave offers promising capabilities for THz-based all-optical particle acceleration and strong-field THz nonlinear physics. However, the lack of highly efficient and frequency-modulable multicycle THz sources is impeding the spread of strong-field THz science and applications. Here, we show that by simply adding an echelon into a single-cycle THz source based on optical rectification in lithium niobate crystals via the tilted pulse-front technique, multicycle THz pulses can be efficiently generated with an 800 nm-to-THz efficiency of 0.1% at room temperature. The radiated THz properties can be engineered by precisely designing the echelon structure. Our proposed multicycle THz generation method has the advantages of high efficiency, ease of operation, and quick switching between single-cycle and multicycle working modes, all of which are important in the application of high-field THz radiation.
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http://dx.doi.org/10.1364/OL.456498DOI Listing
June 2022

MS-FINDER Assisted in Understanding the Profile of Flavonoids in Temporal Dimension during the Fermentation of Pu-erh Tea.

J Agric Food Chem 2022 Jun 30;70(23):7085-7094. Epub 2022 May 30.

State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Avenida Wai Long, Taipa 999078, Macau, SAR, China.

Flavonoid represents a significant class of secondary metabolites in Pu-erh tea with benefits to human health. For a rapid and complete discovery of such compounds, we established a data mining workflow that integrates software MS-DIAL, MS-FINDER, and molecular networking analysis. As a result, 181 flavonoids were tentatively annotated including 22 first found in Pu-erh tea, and two of them were potentially new molecules. The dynamic alteration of these flavonoids during Pu-erh fermentation was further investigated. They all showed a trend of first increasing and then decreasing. Moreover, statistical analysis showed that the first to third pile turnings of the fermentation process had a greater impact on the changes of flavonoids. Partial metabolic pathways were proposed. This study provides a quick and automatic strategy for flavonoid profiling. The temporal dimension of flavonoids during fermentation may serve as a theoretical basis for Pu-erh tea manufacturing technology and study on substance foundation.
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http://dx.doi.org/10.1021/acs.jafc.2c01595DOI Listing
June 2022
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