Publications by authors named "Xuan Li"

1,171 Publications

  • Page 1 of 1

Simultaneous Stenting for Symptomatic Tandem Extracranial and Intracranial Posterior Circulation Stenoses: Long-Term Outcomes and Procedural Experience.

Front Neurol 2021 31;12:724985. Epub 2021 Aug 31.

Department of Interventional Radiology and Vascular Surgery, Peking University Third Hospital, Beijing, China.

Few studies have reported on simultaneous endovascular stenting for tandem posterior circulation (PC) stenoses and its long-term outcomes. Thus, our aim was to investigate the safety and efficacy of simultaneous stenting in patients with symptomatic tandem extra- and intracranial PC stenoses. From September 2014 to June 2018, 16 such patients with symptomatic stenoses who underwent simultaneous stent placement were analyzed. The primary outcome was occurrence of any stroke, TIA, or death within 30 days after the procedure. The secondary outcomes were technical success, clinical success, and the occurrence of in-stent restenosis ≥50% during follow-up. Technical success was defined as stent coverage of all tandem lesions and residual stenosis <30%. Clinical success was determined based on any occurrence of neurological events or death within 3 months after the procedure. All stents (19 intracranial and 14 extracranial) were placed with a technical success rate of 100%. One patient experienced a pontine ischemic stroke 2 days after the procedure and had recovered well at discharge. One patient experienced a minor complication of groin hematoma. The clinical success rate was 93.75% (15/16). During a median follow-up of 36.0 ± 11.0 months, two patients developed ISR ≥50% at the 1-year follow-up. None of the patients experienced stroke, TIA, or death after discharge during follow-up. Simultaneous stenting for symptomatic tandem extra- and intracranial PC stenoses is safe and feasible. Its impact on long-term stroke prevention is promising, and further study of a larger patient population is needed.
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http://dx.doi.org/10.3389/fneur.2021.724985DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438130PMC
August 2021

Fixed duration prolonged duration treatment after first line therapy in patients with systemic light chain amyloidosis.

Amyloid 2021 Sep 14:1-8. Epub 2021 Sep 14.

Department of Medical Oncology and Hematology, Princess Margaret Cancer Centre, Toronto, Canada.

Background: The main objective of treatment in systemic light chain amyloidosis (AL amyloidosis) is to achieve the best hematological response. Deeper responses are associated with better organ responses and survival. In this study, we analysed the efficacy of prolonged duration treatment after first line in patients with AL amyloidosis.

Methods: Retrospective analysis that included patients older than 18 years with AL amyloidosis. We excluded patients with more than 30% marrow plasmacytosis or concurrent multiple myeloma. Two cohorts identified accordingly if they received or not prolonged treatment after the first line. Survival analysis regarding progression free survival (PFS) and overall survival (OS) estimated with Kaplan-Meier and comparisons between groups with log-rank.

Results: Thirty-eight patients were included in the analysis with a median age of 55 years. Twenty-one patients received prolonged duration treatment and 17 did not. In the prolonged duration group, after a median duration of 12 months, the median PFS was 58.8 months. In the fixed duration treatment group, PFS was 30.6 months. The difference was significant with  = .0045 favouring prolonged duration treatment. Organ response was sustained for a longer period in the prolonged duration treatment group. For OS, the difference was not significant.

Conclusions: Prolonged duration treatment in patients with systemic light chain amyloidosis correlated with better PFS and deeper organ responses. Prospective studies are needed to analyse this further.
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http://dx.doi.org/10.1080/13506129.2021.1978423DOI Listing
September 2021

Latent-transforming growth factor β-binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase-3.

Exp Ther Med 2021 Oct 9;22(4):1146. Epub 2021 Aug 9.

Cardiovascular Department, Jinqiu Hospital of Liaoning Province, Shenyang, Liaoning 110016, P.R. China.

Cardiac fibrosis is a core process in the development of heart failure. However, the underlying mechanism of cardiac fibrosis remains unclear. Recently, a study found that in an isoproterenol (ISO)-induced cardiac fibrosis animal model, there is high expression of latent-transforming growth factor β-binding protein 2 (LTBP2) in cardiac fibroblasts. Whether LTBP2 serves a role in cardiac fibrosis is currently unknown. In the present study, mouse cardiac fibroblasts (MCFs) were treated with 100 µM/l ISO for 24, 48, or 72 h, and small interfering RNAs (siRNAs) were used to knockdown LTBP2. Reverse transcription-quantitative PCR and western blotting were used to determine gene and protein expression levels, respectively. Caspase-3 serves a key role in cell apoptosis and is related to cardiac fibrosis-induced heart failure. Caspase-3 activity was therefore determined using a caspase-3 assay kit, CCK8 was used to determine the rate of cell proliferation and apoptosis rates were quantified using a cell death detection ELISA kit. The present study demonstrated that cell apoptosis and LTBP2 expression increased in MCFs treated with 100 µM/l ISO in a time-dependent manner. Expression and activity of caspase-3 also increased in MCFs treated with 100 µM/l ISO for 48 h compared with the control group. In addition, ISO stimulation-induced MCF apoptosis, along with the increased expression of caspase-3 were partly abolished when LTBP2 was knocked down. In conclusion, LTBP2 expression increased in ISO-treated MCFs and accelerated mouse cardiac fibroblast apoptosis by enhancing the expression and activity of caspase-3. LTBP2 may therefore be a potential therapeutic target for treating patients with cardiac fibrosis.
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http://dx.doi.org/10.3892/etm.2021.10580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393931PMC
October 2021

[Expression and Significance of Leucine-rich Repeat-containing G-protein Coupled Receptor 5/6 in Wnt Pathway in Children with Acute Lymphoblastic Leukemia].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2021 Aug;43(4):595-602

Department of Pediatrics,The Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu 221002,China.

Objective To study the expression and significance of leucine-rich repeat-containing G-protein coupled receptor(LGR)5/6 in childhood acute lymphoblastic leukemia(ALL). Methods A total of 39 children who had ALL and achieved complete remission on day 33 after induction therapy were enrolled.The children before induction therapy were considered as the incipient group,and those who achieved complete remission on day 33 by induction therapy were considered as the remission group.According to the degree of risk,they were assigned into 3 groups:low-risk(=16),intermediate-risk(=9),and high-risk(=14)groups.A total of 30 children with immune thrombocytopenia were taken as the control group.From each child in the incipient group,remission group,and control group,3 ml bone marrow sample was collected.Real-time fluorescent quantitative polymerase chain reaction was conducted to measure the mRNA expression of LGR5 and LGR6 in the blood cells of bone marrow.Western blot was employed to measure the protein expression of LGR5 and LGR6 in blood cells of bone marrow. Results Compared with the control(mRNA:1.541±0.409,protein:0.138±0.041)and remission(mRNA:1.418±0.324,protein:0.130±0.033)groups,the incipient group had significantly lower mRNA(0.850±0.279)and protein(0.083±0.027)expression of LGR5(=0.000,=0.000).Compared with the control(mRNA:0.928±0.373,protein:0.094±0.037)and remission(mRNA:0.886±0.390,protein:0.111±0.039)groups,the incipient group had significantly higher mRNA(2.444±1.160)and protein(0.298±0.088)expression of LGR6(=0.000,=0.000).In the incipient groups,low-risk children showed significantly higher mRNA(1.004±0.284)and protein(0.097±0.030)expression of LGR5 than the intermediate-risk children(mRNA:0.728±0.239,protein:0.071±0.022)and high-risk children(mRNA:0.752±0.222,protein:0.074±0.020)(=0.012,=0.016);low-risk children showed significantly lower mRNA(1.822±0.979)and protein(0.245±0.077)expression of LGR6 than the intermediate-risk children(mRNA:2.954±1.039,protein:0.338±0.081)and high-risk children(mRNA:2.827±1.165,protein:0.333±0.075)(=0.016,=0.004).In the remission groups,low-risk children showed significantly higher mRNA(1.597±0.329)and protein(0.150±0.035)expression of LGR5 than the intermediate-risk children(mRNA:1.277±0.288,protein:0.117±0.029)and high-risk children(mRNA:1.305±0.253,protein:0.116±0.023)(=0.012,=0.006);low-risk children showed significantly lower mRNA(0.662±0.334)and protein(0.089±0.034)expression of LGR6 than the intermediate-risk children(mRNA:1.066±0.273,protein:0.130±0.033)and high-risk children(mRNA:1.027±0.405,protein:0.126±0.038)(=0.007,=0.007). Conclusion The expression of LGR5 and LGR6 are closely related to the occurrence and risk of childhood ALL,but its mechanism needs further study.
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http://dx.doi.org/10.3881/j.issn.1000-503X.13645DOI Listing
August 2021

Effects of steam-flaked grains on foals' growth and faecal microbiota.

BMC Vet Res 2021 Sep 4;17(1):293. Epub 2021 Sep 4.

College of Animal Science, Xinjiang Key Laboratory of Herbivore Nutrition for Meat & Milk Production, Xinjiang Agricultural University, Xinjiang, 830052, Urumqi, China.

Background: There is little objective information concerning the effect of steam-flaked grains on foal's growth performance and faecal microbiota. To determine the effects of steam-flaked grains on foal's growth performance and faecal microbiota, faecal samples were collection from 18 foals which had been fed either corn, oat or barley diets over the 60 days of the experiment. Body weight and conformation measurements were collected. Next-generation sequencing of the V3 + V4 region of the 16 S rRNA gene was used to assess the microbial composition of faeces. Alpha diversity, Venn graph, Relative abundance and beta diversity are presented.

Results: There was a significantly higher larger increase in the body weight of those foals fed barley compared to either corn or oats. There were also significant changes in the Alpha diversity of the gut microbiota. The Shannon and Simpson indices were significantly higher in the barley fed group than those fed corn or oats. The Chao1 index was significantly higher in the oat fed group than the corn or barley fed groups. There were significant changes in the relative abundance of bacteria in the microbiota in terms of phylum, family and genus. The histogram of LDA value distribution showed that the 12 statistically different biomarkers of the bacteria were present. Tax4Fun function annotation clustering heat map showed that functional information was detected from 26 species of bacteria in faecal samples from the foals.

Conclusions: Differences by starch sources were found in overall growth of the foals and in the faecal microbiota if either supplementary corn, oat or barley was fed. Further studies are required to determine the potential impact of the changes in the microbiota on the health and development of foals fed cereal starch of different sources.
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http://dx.doi.org/10.1186/s12917-021-02994-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418754PMC
September 2021

Disrupted functional connectivity in white matter resting-state networks in unilateral temporal lobe epilepsy.

Brain Imaging Behav 2021 Sep 3. Epub 2021 Sep 3.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for Neuroinformation, Center for Information in Medicine, School of Life Science and Technology, University of Electronic Science and Technology of China, Second North Jianshe Road, Chengdu, 610054, People's Republic of China.

Unilateral temporal lobe epilepsy (TLE) is the most common type of focal epilepsy characterized by foci in the unilateral temporal lobe grey matters of regions such as the hippocampus. However, it remains unclear how the functional features of white matter are altered in TLE. In the current study, resting-state functional magnetic resonance imaging (fMRI) was performed on 71 left TLE (LTLE) patients, 79 right TLE (RTLE) patients and 47 healthy controls (HC). Clustering analysis was used to identify fourteen white matter networks (WMN). The functional connectivity (FC) was calculated among WMNs and between WMNs and grey matter. Furthermore, the FC laterality of hemispheric WMNs was assessed. First, both patient groups showed decreased FCs among WMNs. Specifically, cerebellar white matter illustrated decreased FCs with the cerebral superficial WMNs, implying a dysfunctional interaction between the cerebellum and the cerebral cortex in TLE. Second, the FCs between WMNs and the ipsilateral hippocampus (grey matter foci) were also reduced in patient groups, which may suggest insufficient functional integration in unilateral TLE. Interestingly, RTLE showed more severe abnormalities of white matter FCs, including links to the bilateral hippocampi and temporal white matter, than LTLE. Taken together, these findings provide functional evidence of white matter abnormalities, extending the understanding of the pathological mechanism of white matter impairments in unilateral TLE.
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http://dx.doi.org/10.1007/s11682-021-00506-8DOI Listing
September 2021

The influence of alternating cyclic dynamic loads with different low frequencies on the bio-corrosion behaviors of AZ31B magnesium alloy .

Bioact Mater 2022 Jan 11;7:263-274. Epub 2021 Jun 11.

School of Materials Science and Engineering, Southeast University, Nanjing, 211189, Jiangsu, China.

The real physiological environment of human body is complicated with different degrees and forms of dynamic loads applied to implanted medical devices due to the daily activities of the patients, which would have impacts on the degradation behaviors of magnesium alloy implants. In this work, the bio-corrosion behaviors of AZ31B magnesium alloy under alternating cyclic dynamic loads with different low frequencies (0.1-2.5 Hz) were specially investigated. It was found that the bio-degradation performances under external dynamic stressed conditions were much severer than those under unstressed conditions and static loads. The corrosion rates were generally accelerated as the rise of cyclic frequency. Hereby a numerical model for the degradation process of Mg alloy was established. The corrosion current density of Mg alloy and the applied loading frequency matches a linear relationship of , which is the result of interactions between the cyclic alternating load and corrosive environment. This work could provide a theoretical reference and an experimental basis for further researches on the biodegradation behaviors of biomedical materials under dynamic conditions.
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http://dx.doi.org/10.1016/j.bioactmat.2021.05.049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379482PMC
January 2022

Effects of Inhaled Corticosteroid/long-acting beta-2 Agonist Combination on the Airway Microbiome of Patients with COPD: A Randomized Controlled Trial (DISARM).

Am J Respir Crit Care Med 2021 Aug 31. Epub 2021 Aug 31.

University of Alberta, 3158, Medicine, Edmonton, Alberta, Canada;

Rationale: Inhaled corticosteroids (ICS) are commonly prescribed with long-acting beta-2 agonists (LABA) in COPD. To date, the effects of ICS therapy on the airway microbiome in COPD are unknown.

Objectives: To determine the effects of ICS/LABA on the airway microbiome of COPD patients.

Methods: Clinically stable COPD patients were enrolled into a 4-week run-in period during which ICS was discontinued and all participants were placed on formoterol 12 µg twice daily (BID). The participants were then randomized to: budesonide/formoterol (Bud + Form; 400/12 µg BID), fluticasone/salmeterol (Flu + Salm; 250/50 µg BID) or formoterol only (Form; 12 µg BID) for 12 weeks. Participants underwent bronchoscopy before and after the 12-week treatment period. The primary endpoint was the comparison of changes in the airway microbiome over the trial period between the ICS/LABA and LABA-only groups.

Measurements And Main Results: 63 participants underwent randomization: Bud + Form (n=20), Flu + Salm (n=22) and Form (n=21) groups; 56 subjects completed all visits. After the treatment period, changes in alpha diversity were significantly different across groups, especially between Flu + Salm and Form groups (Δ richness: p = 0.02; Δ Shannon Index: p = 0.03). Longitudinal differential abundance analyses revealed more pronounced microbial shifts from baseline in the fluticasone (vs. budesonide or formoterol only) group.

Conclusions: Fluticasone-based ICS/LABA therapy modifies the airway microbiome in COPD, leading to a relative reduction in alpha diversity and a greater number of bacterial taxa changes. These data may have implications in patients who develop pneumonia on ICS. Clinical trial registration available at www.clinicaltrials.gov, ID:NCT02833480.
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http://dx.doi.org/10.1164/rccm.202102-0289OCDOI Listing
August 2021

Solid-phase denitrification in high salinity and low-temperature wastewater treatment.

Bioresour Technol 2021 Aug 19;341:125801. Epub 2021 Aug 19.

School of Civil Engineering, Shandong University, Jinan, Shandong 250002, China. Electronic address:

Nitrogen removal from wastewater is often deteriorated under high salinity and low temperature. Solid-phase denitrification (SPD) might improve total nitrogen removal efficiency (TNRE) by stably supplying carbon resources under adverse conditions. In this study, an SPD biofilm reactor was successfully established by inoculating halophilic sludge and filling poly (butanediol succinate) (PBS) granules, and achieved over 96% TNRE at low temperature. More extracellular polysaccharides were produced at low temperature. Microbial network analysis evidenced dominant heterotrophic denitrifiers (Marinicella, Fusibacter, Saccharicrinis and Vitellibacter) at 25 °C were replaced by genera Melioribacter, Marinobacter, Desulfatitalea and Thiomicrospira at 15 °C. At low temperature, genes nirS and narG might be mainly responsible for denitrification. Fluorescence spectrum coupled with fluorescence regional integration and parallel factor analysis revealed low temperature increased the proportion of proteins of soluble microbial products. This study provides guidance for the practical application of SPD in the treatment of high salinity and low-temperature wastewater.
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http://dx.doi.org/10.1016/j.biortech.2021.125801DOI Listing
August 2021

Evaluation of the Diagnostic Potential of a Plasma Exosomal miRNAs Panel for Gastric Cancer.

Front Oncol 2021 4;11:683465. Epub 2021 Aug 4.

Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Purpose: Gastric cancer (GC) is often difficult to diagnose early in the disease and remains one of the most frequently occurring malignancies. This investigation looks at the diagnostic potential of a specific plasma exosomal miRNAs panel for GC.

Methods: This study analyzed 216 individual peripheral blood samples. 2 GEO datasets were analyzed and two miRNAs were selected - plasma exosomal miR-195-5p and miR-211-5p. Quantitative reverse-transcriptase PCR (qRT-PCR) was used to assess relative expressions and receiver operating characteristic (ROC) curve analysis was used to determine the diagnostic efficiency of miR-195-5p and miR-211-5p panel. The Kaplan-Meier method was used to assess the prognostic value of plasma exosomal miR-195-5p and miR-211-5p.

Results: GC patients possessed notably raised plasma levels of exosomal miR-195-5p and miR-211-5p. The area under ROC curves (AUCs) of miR-195-5p, miR-211-5p were 0.745, 0.798 in the screening phase and 0.762, 0.798 in the training stage respectively. GC was able to be diagnosed more accurately when both miRNAs were interpreted together (AUC=0.820 in the validation stage). Poorer prognosis was observed in GC patients who had plasma exosomal miR-195-5p and miR-211-5p of higher levels. experiments also confirmed that miR-195-5p and miR-211-5p is able to be transmitted between cells, and works to enhance tumor invasion, migration and proliferation while inhibiting cell apoptosis.

Conclusion: Plasma exosomal miR-195-5p and miR-211-5p may become potential biomarkers for GC diagnosis, and may be useful in predicting tumor phenotype.
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http://dx.doi.org/10.3389/fonc.2021.683465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371241PMC
August 2021

Identification of effective natural PIK3CA H1047R inhibitors by computational study.

Aging (Albany NY) 2021 Aug 20;13(16):20246-20257. Epub 2021 Aug 20.

Department of Breast Surgery, The First Hospital of Jilin University, Changchun, China.

Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer with a poor prognosis and a high recurrence rate. gene is frequently mutated in breast cancer, with as the hotspot mutation reported in TNBC. We used the ZINC database to screen natural compounds that could be structurally modified to develop drugs targeting the PIK3CA H1047R mutant protein in the PI3K pathway. The LibDock module showed that 2,749 compounds could strongly bind to the PIK3CA H1047R protein. Ultimately, the top 20 natural ligands with high LibDock scores were used for further analyses including assessment of ADME (absorption, distribution, metabolism, and excretion), toxicity, stability, and binding affinity. ZINC000004098448 and ZINC000014715656 were selected as the safest drug candidates with strong binding affinity to PIK3CA H1047R, no hepatotoxicity, less carcinogenicity, better plasma protein binding (PPB) properties, and enhanced intestinal permeability and absorption than the two reference drugs, PKI-402 and wortmannin. Moreover, their lower potential energies than those of PIK3CA H1047R confirmed the stability of the ligand-receptor complex under physiological conditions. ZINC000004098448 and ZINC000014715656 are thus safe and stable leads for designing drugs against PIK3CA H1047R as part of a targeted therapeutic approach for patients with TNBC.
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http://dx.doi.org/10.18632/aging.203409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436935PMC
August 2021

Spatial distribution of health risks for residents located close to solvent-consuming industrial VOC emission sources.

J Environ Sci (China) 2021 Sep 6;107:38-48. Epub 2021 Feb 6.

Beijing Key Laboratory of Urban Atmospheric Volatile Organic Compounds Pollution Control and Application, Beijing Municipal Research Institute of Environment Protection, Beijing 100037, China. Electronic address:

Emissions derived from the consumption of organic solvents have been proven to be the primary industrial source of volatile organic compounds (VOCs). In conjunction with epidemiologic studies, water-based paints (WBPs) and solvent-based paints (SBPs) were selected as representatives of newly developed solvents and traditional solvents, respectively, to simulate the effects of consuming solvents emitted during industrial production. And non-carcinogenic and carcinogenic risks to residents near emission sources were studied in detail. The results showed that the spatial distribution of health risks varied with meteorological conditions and type of emission source, and the prevailing wind direction strongly affected the distribution range and shape of the influenced area. The areas of influence maximized on heavy-polluting days for both WBP and SBP emission sources with the total span reaching 804 m and 16 km, respectively; meanwhile, the areas of influence for carcinogenic risk resulting from WBP emission sources were 1.2 and 2.3 times greater than those measured on fine and rainy days, respectively, and 1.8 and 2.9 times greater for SBP emission sources. Compared with WBPs, the total spans of negatively influenced regions resulting from SBP emission sources were 10.4, 12.5 and 19.9 times greater on fine, rainy and heavy-polluting days, respectively. Therefore, carcinogenic risk was the dominant health threat for populations residing close to solvent-consuming industrial emission sources. The findings suggest that newly developed solvents are capable of significantly reducing consequent health threats, nevertheless, they could still pose occasional threats to nearby residents under specific meteorological conditions.
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http://dx.doi.org/10.1016/j.jes.2021.01.014DOI Listing
September 2021

Cadmium induced cardiac toxicology in developing Japanese quail (Coturnix japonica): Histopathological damages, oxidative stress and myocardial muscle fiber formation disorder.

Comp Biochem Physiol C Toxicol Pharmacol 2021 Aug 15;250:109168. Epub 2021 Aug 15.

College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China. Electronic address:

The anthropogenic-induced cadmium (Cd) pollution poses great threats to human health and wildlife survival. Birds also suffer from Cd contamination and Cd exerts negative impacts on multiple organs in birds. However, its toxic effects on cardiac organ of birds are still unclear. In this study, one-week old male Japanese quails were exposed to 15, 30, 60 and 75 mg/kg Cd for 5 weeks when birds in control group reached sex maturity. The results showed that Cd could cause microstructural damages including congestion and myocardial fiberolysis. Ultrastructural analysis also showed myocardial muscle fiber disarrangement and rupture as well as mitochondrial swelling, vacuolation and membrane lysis in Cd concentration groups. Moreover, Cd induced oxidative stress in the heart by decreasing antioxidant enzyme activities of catalase (CAT), glutathione peroxidase (GPX), total antioxidant capacity (T-AOC), superoxide dismutase (SOD) while increasing oxidative biomarkers such as malondialdehyde (MDA), inducible nitric oxide synthase (iNOS), and content of nitric oxide (NO). In addition, mRNA expression levels of genes involved in muscle fiber formation signaling pathway such as Follistatin (FST), paired box 3 (PAX3), myogenic differentiation 1 (MYoD1) and SRY-box transcription factor 6 (SOX6), were down-regulated by Cd exposure. Furthermore, PI3K/Akt/mTOR signaling pathway were disrupted by Cd exposure implying energy supply deficiency in the heart. We concluded that Cd caused cardiac dysfunction by inducing heart underdevelopment, histopathological injury, oxidative stress and myocardial muscle fiber formation disruption.
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http://dx.doi.org/10.1016/j.cbpc.2021.109168DOI Listing
August 2021

Probiotics use is associated with improved clinical outcomes among hospitalized patients with COVID-19.

Therap Adv Gastroenterol 2021 4;14:17562848211035670. Epub 2021 Aug 4.

Department of Respiratory and Critical Care Medicine, Affiliated Hospital, Institute of Respiratory Diseases, Guangdong Medical University, Zhanjiang, Guangdong, China.

Background And Aims: Currently, there are no definitive therapies for coronavirus disease 2019 (COVID-19). Gut microbial dysbiosis has been proved to be associated with COVID-19 severity and probiotics is an adjunctive therapy for COIVD-19. However, the potential benefit of probiotics in COVID-19 has not been studied. We aimed to assess the relationship of probiotics use with clinical outcomes in patients with COVID-19.

Methods: We conducted a propensity-score matched retrospective cohort study of adult patients with COVID-19. Eligible patients received either probiotics plus standard care (probiotics group) or standard care alone (non-probiotics group). The primary outcome was the clinical improvement rate, which was compared among propensity-score matched groups and in the unmatched cohort. Secondary outcomes included the duration of viral shedding, fever, and hospital stay.

Results: Among the propensity-score matched groups, probiotics use was related to clinical improvement rates (log-rank  = 0.028). This relationship was driven primarily by a shorter (days) time to clinical improvement [difference, -3 (-4 to -1),  = 0.022], reduction in duration of fever [-1.0 (-2.0 to 0.0),  = 0.025], viral shedding [-3 (-6 to -1),  < 0.001], and hospital stay [-3 (-5 to -1),  = 0.009]. Using the Cox model with time-varying exposure, use of probiotics remained independently related to better clinical improvement rate in the unmatched cohort.

Conclusion: Our study suggested that probiotics use was related to improved clinical outcomes in patients with COVID-19. Further studies are required to validate the effect of probiotics in combating the COVID-19 pandemic.
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http://dx.doi.org/10.1177/17562848211035670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351028PMC
August 2021

Allogeneic stem cell transplantation may overcome the adverse impact of myelofibrosis on the prognosis of myelodysplastic syndrome.

Exp Hematol Oncol 2021 Aug 14;10(1):44. Epub 2021 Aug 14.

Department of Hematology, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Blvd North, Guangzhou, China.

Purpose: Myelofibrosis (MF) may serve as a poor prognostic factor in myelodysplastic syndromes (MDS). This study explored the impact of allogeneic hematopoietic stem cell transplantation (allo-HSCT) on the outcome of MDS patients with MF.

Patients And Methods: Three hundred and sixteen MDS patients were enrolled in this retrospective study. Based on the degree of MF, we divided the patients into 2 groups: grade 0-1 (MF-0/1) and grade 2-3 (MF-2/3) groups. The clinical features, treatments, and prognosis in MDS patients with MF were analyzed.

Results: Forty-three (13.6%) patients were diagnosed as MF-2/3. Complex karyotypes were more common in the MF-2/3 compared to MF-0/1 groups (P = 0.002). The overall response rate (ORR) of cytoreduction was 49.0%, along with 53.3% in the MF-0/1 and 16.7% in MF-2/3 groups (P = 0.017). In total, 141 patients underwent allo-HSCT, including 121 in the MF-0/1 and 20 in MF-2/3 groups. The median time to neutrophil reconstruction was 12 (range: 7-34) and 14 (range: 10-45) days (P = 0.005), and platelet reconstruction was 14 (range: 8-68) and 18 (range: 8-65) days (P = 0.045) in the MF-0/1 and MF-2/3 groups, respectively. However, the cumulative incidence of neutrophil and platelet engraftment achieved at day + 30 was not different between the two groups (P = 0.107, P = 0.303, respectively). Non-relapse mortality, relapse, and acute and chronic graft-versus-host disease were similar between the two groups (all P > 0.05). Among patients with allo-HSCT, the 2-year overall survival (OS) was 68.5% (95% CI: 60.1-76.9%) and 68.4% (95% CI: 47.4-89.4%) in the MF-0/1 and MF-2/3 groups, respectively, (P = 0.636). Among patients without allo-HSCT, the 2-year OS was 49.9% (95% CI: 40.7-59.1%) and 19.2% (95% CI: 0-39.6%) in the MF-0/1 and MF-2/3 groups, respectively, (P = 0.002). In multivariate cox analysis, complex karyotype was an unfavorable factor for relapse (HR, 4.16; P = 0.006), disease-free survival (DFS) (HR, 2.16; P = 0.020), and OS (HR, 2.47; P = 0.009) post-transplantation.

Conclusion: Patients with MF-2/3 have more complex karyotypes and lower ORR of cytoreduction in MDS. Among patients without allo-HSCT, patients with MF-2/3 have a worse prognosis than those with MF-0/1. However, the adverse impact of MF on prognosis may be overcome by allo-HSCT.
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http://dx.doi.org/10.1186/s40164-021-00238-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364708PMC
August 2021

Development of functional connectivity within and among the resting-state networks in anesthetized rhesus monkeys.

Neuroimage 2021 Nov 12;242:118473. Epub 2021 Aug 12.

Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuchang District, Wuhan, Hubei 430071, China. Electronic address:

Objective: The age-related changes in the resting-state networks (RSNs) exhibited temporally specific patterns in humans, and humans and rhesus monkeys have similar RSNs. We hypothesized that the RSNs in rhesus monkeys experienced similar developmental patterns as humans.

Methods: We acquired resting-state fMRI data from 62 rhesus monkeys, which were divided into childhood, adolescence, and early adulthood groups. Group independent component analysis (ICA) was used to identify monkey RSNs. We detected the between-group differences in the RSNs and static, dynamic, and effective functional network connections (FNCs) using one-way variance analysis (ANOVA) and post-hoc analysis.

Results: Eight rhesus RSNs were identified, including cerebellum (CN), left and right lateral visual (LVN and RVN), posterior default mode (pDMN), visuospatial (VSN), frontal (FN), salience (SN), and sensorimotor networks (SMN). In internal connections, the CN, SN, FN, and SMN mainly matured in early adulthood. The static FNCs associated with FN, SN, pDMN primarily experienced fast descending slow ascending type (U-shaped) developmental patterns for maturation, and the dynamic FNCs related to pDMN (RVN, CN, and SMN) and SMN (CN) were mature in early adulthood. The effective FNC results showed that the pDMN and VSN (stimulated), SN (inhibited), and FN (first inhibited then stimulated) chiefly matured in early adulthood.

Conclusion: We identified eight monkey RSNs, which exhibited similar development patterns as humans. All the RSNs and FNCs in monkeys were not widely changed but fine-tuned. Our study clarified that the progressive synchronization, exploration, and regulation of cognitive RSNs within the pDMN, FN, SN, and VSN denoted potential maturation of the RSNs throughout development. We confirmed the development patterns of RSNs and FNCs would support the use of monkeys as a best animal model for human brain function.
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http://dx.doi.org/10.1016/j.neuroimage.2021.118473DOI Listing
November 2021

One-Pot Facile Synthesis of Double-Shelled Mesoporous Silica Microcapsules with an Improved Soft-Template Method for Sustainable Pest Management.

ACS Appl Mater Interfaces 2021 Aug 13;13(33):39066-39075. Epub 2021 Aug 13.

College of Plant Protection, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China.

A controlled release formulation based on silica microcapsules is an ideal selection to improve both the effective utilization and duration of pesticides to decrease ecological damage. Herein, a simple and green method for preparing double-shelled microcapsules was developed using a newly prepared quaternary ammonium ionic liquid (IL) as the functional additive to entrap avermectin (Ave) in mesoporous silica nanospheres (MSNs) and tannic acid-Cu (TA-Cu) complex as the sealing agent to form the core-shell structure ([email protected]@TA-Cu). The obtained microcapsules with an average size of 538 nm had pH-responsive release property and good stability in soil. The half-life of microcapsules (34.66 days) was 3 times that of Ave emulsifiable concentrate (EC) (11.55 days) in a test soil, which illustrated that microcapsules could protect Ave from rapid degradation by microorganisms by releasing TA, copper, and quaternary ammonium in the soil. [email protected]@TA-Cu microcapsules had better nematicidal activity and antibacterial activity than Ave EC due to the synergistic effect of Ave, IL, and copper incorporated in the microcapsules. Pot experiments showed that the control efficacy of microcapsules was 87.10% against , which is better than that of Ave EC (41.94%) at the concentration of 1.0 mg/plant by the root-irrigation method after 60 days of treatment owing to the extended duration of Ave in microcapsules. The simple and green method for the preparation of double-shelled microcapsules based on natural quaternary ammonium IL would have tremendous potential for the extensive development of controlled release pesticide formulations.
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http://dx.doi.org/10.1021/acsami.1c10135DOI Listing
August 2021

A novel index of functional connectivity: phase lag based on Wilcoxon signed rank test.

Cogn Neurodyn 2021 Aug 4;15(4):621-636. Epub 2020 Nov 4.

Co-innovation Center of Shandong Colleges and Universities: Future Intelligent Computing, Shandong Technology and Business University, Yantai, 264005 Shandong People's Republic of China.

Phase synchronization has been an effective measurement of functional connectivity, detecting similar dynamics over time among distinct brain regions. However, traditional phase synchronization-based functional connectivity indices have been proved to have some drawbacks. For example, the phase locking value (PLV) index is sensitive to volume conduction, while the phase lag index (PLI) and the weighted phase lag index (wPLI) are easily affected by noise perturbations. In addition, thresholds need to be applied to these indices to obtain the binary adjacency matrix that determines the connections. However, the selection of the thresholds is generally arbitrary. To address these issues, in this paper we propose a novel index of functional connectivity, named the phase lag based on the Wilcoxon signed-rank test (PLWT). Specifically, it characterizes the functional connectivity based on the phase lag with a weighting procedure to reduce the influence of volume conduction and noise. Besides, it automatically identifies the important connections without relying on thresholds, by taking advantage of the framework of the Wilcoxon signed-rank test. The performance of the proposed PLWT index is evaluated on simulated electroencephalograph (EEG) datasets, as well as on two resting-state EEG datasets. The experimental results on the simulated EEG data show that the PLWT index is robust to volume conduction and noise. Furthermore, the brain functional networks derived by PLWT on the real EEG data exhibit a reasonable scale-free characteristic and high test-retest (TRT) reliability of graph measures. We believe that the proposed PLWT index provides a useful and reliable tool to identify the underlying neural interactions, while effectively diminishing the influence of volume conduction and noise.
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http://dx.doi.org/10.1007/s11571-020-09646-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286916PMC
August 2021

Mesenchymal Stem Cells Resist Mechanical Confinement through the Activation of the Cortex during Cell Division.

ACS Biomater Sci Eng 2021 09 8;7(9):4602-4613. Epub 2021 Aug 8.

Key Laboratory of Biorheological Science and Technology, Ministry of Education College of Bioengineering, Chongqing University, Chongqing 400044, China.

The mechanical properties of the natural extracellular matrix (ECM) change extensively, but these specific properties provide a relatively stable environment for resident cells. Although the effect of matrix stiffness on cell functions has been widely studied, the molecular mechanism was still not fully understood. Matrix stiffening is a common phenomenon in tissue damaging processes. To explore the effect of the increase in local matrix stiffness on cell behaviors, a three-dimensional (3D) cell culture system with a tunable modulus but constant other physical parameters was constructed by the alginate hydrogel with different molecular weights and cross-linking degrees. By using this culture system, the transcriptome response of mesenchymal stem cells (MSCs) to matrix stiffness was explored. Furthermore, a finite element model was developed to simulate the interaction between cells and the matrix. Results revealed that the increased matrix stiffness promoted the proliferation-related signaling of MSCs, and this process depended on the increased cortex tension caused by the activation of RAS and myosin II.
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http://dx.doi.org/10.1021/acsbiomaterials.1c00862DOI Listing
September 2021

Elucidation and Regulation of Polyphenols in the Smoking Process of Shanxi Aged Vinegar.

Foods 2021 Jul 1;10(7). Epub 2021 Jul 1.

Key Laboratory of Industrial Fermentation Microbiology Ministry of Education, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, State Key Laboratory of Food Nutrition and Safety, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.

Polyphenols (PPs) are the main contributors to the health functions of Shanxi aged vinegar (SAV) and are mainly produced during the smoking process. This study aimed to explore the feasibility of regulating the accumulation of total water-soluble PPs (TWSP) by changing environmental factors based on the distribution of PPs. A total of eleven PPs, such as vanillin, vanillic acid, and (e)-ferulic acid, were detected during the smoking process. During the smoking process, the content of TWSP gradually increased and was accompanied by changes in environmental factors. Spearman correlation analysis and verification experiments showed that temperature, amino acids, and reducing sugars, as the main influencing factors, promoted the accumulation of TWSP. The in situ regulation strategy of changing environmental factors significantly increased the accumulation of TWSP by 12.24%.
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http://dx.doi.org/10.3390/foods10071518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305182PMC
July 2021

Semi-continuous anaerobic digestion of secondary sludge with free ammonia pretreatment: Focusing on volatile solids destruction, dewaterability, pathogen removal and its implications.

Water Res 2021 Sep 31;202:117481. Epub 2021 Jul 31.

Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia. Electronic address:

Our previous work has reported the pretreatment of secondary sludge with free ammonia (NH, FA) enhanced the methane production in batch biochemical methane potential tests. However, the batch biochemical methane potential test could only provide conservative results compared to continuous/semi-continuous anaerobic digestion. Also, the impacts of FA pretreatment on the key anaerobic digestion parameters, including volatile solids (VS) destruction, sludge dewaterability and pathogen removal, are still unknown. This study for the first time investigated these impacts using semi-continuous anaerobic digestion systems for 130 days. Pretreatment of secondary sludge for 24 h at an FA concentration of 560 mg NH-N/L improved VS destruction by 26.4% (from 22.0 to 27.8%), supported by a similar increase of 28.6% in methane production (from 126.7 to 162.9 ml CH4/g VS). Model based analysis revealed that FA pretreatment improved the sludge degradability extent, which may be the reason for the enhanced VS destruction. Equally importantly, the dewaterability of the digested sludge with FA pretreatment was also enhanced by 9.2% (from 12.0 to 13.1% in solids content of the dewatered digested sludge), which could be partly attributed to the increased zeta potential from -16.7 to -14.5 mV. Anaerobic digestion with FA pretreatment enhanced the removals of Fecal Coliform and E. Coli by 1.3 and 1.4 log MPN/g TS (MPN: Most Probable Number; TS: Total Solids), indicating FA pretreatment was effective in enhancing pathogen removal. With inorganic solids representing 21% of the sludge used, the volume of dewatered sludge to be disposed of was reduced by 14.5% via FA pretreatment. This will substantially decrease the cost as evaluated by economic analysis. In brief, this study provides a promising strategy to enhance sludge reduction in anaerobic digestion and is of great significance in promoting the application of FA pretreatment strategy in the real world.
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http://dx.doi.org/10.1016/j.watres.2021.117481DOI Listing
September 2021

SARS-CoV-2 and other pathogens in municipal wastewater, landfill leachate, and solid waste: A review about virus surveillance, infectivity, and inactivation.

Environ Res 2021 Aug 4;203:111839. Epub 2021 Aug 4.

School of Civil, Mining and Environmental Engineering, University of Wollongong, Australia; Illawarra Health and Medical Research Institute (IHMRI), University of Wollongong, Wollongong, Australia. Electronic address:

This review discusses the techniques available for detecting and inactivating of pathogens in municipal wastewater, landfill leachate, and solid waste. In view of the current COVID-19 pandemic, SARS-CoV-2 is being given special attention, with a thorough examination of all possible transmission pathways linked to the selected waste matrices. Despite the lack of works focused on landfill leachate, a systematic review method, based on cluster analysis, allows to analyze the available papers devoted to sewage sludge and wastewater, allowing to focalize the work on technologies able to detect and treat pathogens. In this work, great attention is also devoted to infectivity and transmission mechanisms of SARS-CoV-2. Moreover, the literature analysis shows that sewage sludge and landfill leachate seem to have a remote chance to act as a virus transmission route (pollution-to-human transmission) due to improper collection and treatment of municipal wastewater and solid waste. However due to the incertitude about virus infectivity, these possibilities cannot be excluded and need further investigation. As a conclusion, this paper shows that additional research is required not only on the coronavirus-specific disinfection, but also the regular surveillance or monitoring of viral loads in sewage sludge, wastewater, and landfill leachate. The disinfection strategies need to be optimized in terms of dosage and potential adverse impacts like antimicrobial resistance, among many other factors. Finally, the presence of SARS-CoV-2 and other pathogenic microorganisms in sewage sludge, wastewater, and landfill leachate can hamper the possibility to ensure safe water and public health in economically marginalized countries and hinder the realization of the United Nations' sustainable development goals (SDGs).
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http://dx.doi.org/10.1016/j.envres.2021.111839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8332740PMC
August 2021

T 2 cytokines and Staphylococcus aureus cooperatively induce atopic dermatitis-like transcriptomes.

Allergy 2021 Aug 6. Epub 2021 Aug 6.

Laboratory of Investigative Dermatology, The Rockefeller University, New York, NY, USA.

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http://dx.doi.org/10.1111/all.15035DOI Listing
August 2021

The changing epidemiology of hemorrhagic fever with renal syndrome in Southeastern China during 1963-2020: A retrospective analysis of surveillance data.

PLoS Negl Trop Dis 2021 Aug 6;15(8):e0009673. Epub 2021 Aug 6.

Key Laboratory of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.

Background: Hemorrhagic fever with renal syndrome (HFRS) is a rodent-borne disease caused by hantavirus which was endemic Zhejiang Province, China. In this study, we aim to explore the changing epidemiology of HFRS in Zhejiang, identify high-risk areas and populations, and evaluate relevant policies and interventions to better improve HFRS control and prevention.

Methods: Surveillance data on HFRS during 1963-2020 in Zhejiang Province were extracted from Zhejiang Provincial Center for Disease Control and Prevention archives and the Chinese Notifiable Disease Reporting System. The changing epidemiological characteristics of HFRS including seasonal distribution, geographical distribution, and demographic features, were analyzed using joinpoint regression, autoregressive integrated moving average model, descriptive statistical methods, and Spatio-temporal cluster analysis.

Results: From 1963 to 2020, 114 071 HFRS cases and 1269 deaths were reported in Zhejiang Province. The incidence increased sharply from 1973 and peaked in 1986, then decreased steadily and maintained a stable incidence from 2004. HFRS cases were reported in all 11 prefecture-level cities of Zhejiang Province from 1963 to 2020. The joint region (Shengzhou, Xinchang, Tiantai, and surrounding areas), and Kaihua County are the most seriously affected regions throughout time. After 1990, the first HFRS incidence peak was in May-June, with another one from November to January. Most HFRS cases occurred in 21- (26.48%) and 30- years group (24.25%) from 1991 to 2004, but 41- (25.75%) and 51-years (23.30%) had the highest proportion from 2005 to 2020. Farmers accounted for most cases (78.10%), and cases are predominantly males with a male-to-female ratio of 2.6:1. It was found that the median time from onset to diagnosis was 6.5 days (IQR 3.75-10.42), and the time from diagnosis to disease report was significantly shortened after 2011.

Conclusions: We observed dynamic changes in the seasonal distribution, geographical distribution, and demographic features of HFRS, which should be well considered in the development of control and prevention strategies in future. Additional researches are warranted to elucidate the environmental, meteorological, and social factors associated with HFRS incidence in different decades.
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http://dx.doi.org/10.1371/journal.pntd.0009673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372920PMC
August 2021

Potato growth, photosynthesis, yield, and quality response to regulated deficit drip irrigation under film mulching in a cold and arid environment.

Sci Rep 2021 Aug 5;11(1):15888. Epub 2021 Aug 5.

College of Water Conservancy and Hydropower Engineering, Gansu Agricultural University, Lanzhou, 730070, China.

The effects of the amount and timing of regulated deficit drip irrigation under plastic film on potato ('Qingshu 168') growth, photosynthesis, yield, water use efficiency, and quality were examined from 2017 to 2019 in cold and arid northwestern China. In the four stages of potato growth (seedling, tuber initiation, tuber bulking, starch accumulation), eight treatments were designed, with a mild deficit was in treatments WD1 (seedling), WD2 (tuber initiation), WD3 (tuber bulking), and WD4 (starch accumulation); and a moderate deficit in WD5 (seedling), WD6 (tuber initiation), WD7 (tuber bulking), and WD8 (starch accumulation). The net photosynthetic rate, stomatal conductance, and transpiration rate decreased significantly under water deficit in the tuber formation and starch accumulation stages. Although water deficit reduced potato yields, a mild deficit in the seedling stage resulted in the highest yield and water use efficiency at 43,961.91 kg ha and 8.67 kg m, respectively. The highest overall quality was in potatoes subjected to mild and moderate water deficit in the seedling stage. Principal component analysis identified mild water stress in the seedling stage as the optimum regulated deficit irrigation regime. The results of this study provide theoretical and technical references for efficient water-saving cultivation and industrialization of potato in northwestern China.
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http://dx.doi.org/10.1038/s41598-021-95340-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342503PMC
August 2021

Microplastics deteriorate the removal efficiency of antibiotic resistance genes during aerobic sludge digestion.

Sci Total Environ 2021 Jul 30;798:149344. Epub 2021 Jul 30.

Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia. Electronic address:

Sludge from wastewater treatment plants (WWTPs) is considered to be reservoirs of antibiotic resistance genes (ARGs), which can be efficiently removed by sludge treatment processes, e.g., aerobic sludge digestion. However, recent studies report microplastics, which also accumulate in sludge, may serve as carriers for ARGs. In the presence of microplastics, whether ARGs can still be efficiently destroyed by aerobic sludge digestion remains to be urgently investigated. In this study, the fate of ARGs during aerobic digestion was investigated with and without the addition of three prevalent categories of (i.e., polyvinyl chloride (PVC), polyethylene (PE), and polyethylene terephthalate (PET)). Nine ARGs and class 1 integron-integrase gene (intI1) that represents the horizontal transfer potential of ARGs were tested in this study. Compared with the control, the ARGs removal efficiency decreased by 129.6%, 137.0%, and 227.6% with the presence of PVC, PE, and PET, respectively, although a negligible difference was observed with their solids reduction efficiencies. The abundance of potential bacterial hosts of ARGs and intI1 increased in the reactors with the addition of microplastics, suggesting that microplastics potentially selectively enriched bacterial hosts and promoted the horizontal transfer of ARGs during aerobic sludge digestion. These may have contributed to the deteriorated ARGs removal efficiency. This study demonstrated that microplastics in sludge would decrease the ARGs removal efficiency in aerobic digestion process, potentially leading to more ARGs entering the local environment during sludge disposal or utilization.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149344DOI Listing
July 2021

3D M-Net: Object-Specific 3D Segmentation Network Based on a Single Projection.

Comput Intell Neurosci 2021 12;2021:5852595. Epub 2021 Jul 12.

State Key Lab for Electronic Testing Technology, North University of China, Taiyuan 030051, China.

The internal assembly correctness of industrial products directly affects their performance and service life. Industrial products are usually protected by opaque housing, so most internal detection methods are based on X-rays. Since the dense structural features of industrial products, it is challenging to detect the occluded parts only from projections. Limited by the data acquisition and reconstruction speeds, CT-based detection methods do not achieve real-time detection. To solve the above problems, we design an end-to-end single-projection 3D segmentation network. For a specific product, the network adopts a single projection as input to segment product components and output 3D segmentation results. In this study, the feasibility of the network was verified against data containing several typical assembly errors. The qualitative and quantitative results reveal that the segmentation results can meet industrial assembly real-time detection requirements and exhibit high robustness to noise and component occlusion.
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http://dx.doi.org/10.1155/2021/5852595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292052PMC
August 2021

Leveraging Health Information Technology to Collect Family Cancer History: A Systematic Review and Meta-Analysis.

JCO Clin Cancer Inform 2021 Jun;5:775-788

Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Weill Cornell Medicine, New York, NY.

Purpose: Collection of family cancer histories (FCHs) can identify individuals at risk for familial cancer syndromes. The aim of this study is to evaluate the literature on existing strategies whereby providers use information technology to assemble FCH.

Methods: A systematic search of online databases (Ovid MEDLINE, Cochrane, and Embase) between 1980 and 2020 was performed. Statistical heterogeneity was assessed through the chi-square test (ie, Cochrane Q test) and the inconsistency statistic (I). A random-effects analysis was used to calculate the pooled proportions and means.

Results: The comprehensive search produced 4,005 publications. Twenty-eight studies met inclusion criteria. Twenty-seven information technology tools were evaluated. Eighteen out of 28 studies were electronic surveys administered before visits (18, 64.3%). Five studies administered tablet surveys in offices (5, 17.8%). Four studies collected electronic survey via kiosk before visits (4, 14.3%), and one study used animated virtual counselor during visits (1, 3.6%). Among the studies that use an FCH tool, the pooled estimate of the overall completion rate was 86% (CI, 72% to 96%), 84% (CI, 65% to 97%) for electronic surveys before visits, 89% (CI, 0.74 to 0.98) for tablet surveys, and 85% (CI, 0.66 to 0.98) for surveys via kiosk. Mean time required for completion was 31.0 minutes (CI, 26.1 to 35.9), and the pooled estimate of proportions of participants referred to genetic testing was 12% (CI, 4% to 23%).

Conclusion: Our review found that electronic FCH collection can be completed successfully by patients in a time-efficient manner with high rates of satisfaction.
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http://dx.doi.org/10.1200/CCI.21.00004DOI Listing
June 2021

Identification of a Three-RNA Binding Proteins (RBPs) Signature Predicting Prognosis for Breast Cancer.

Front Oncol 2021 12;11:663556. Epub 2021 Jul 12.

Department of Breast Surgery, Key Laboratory of Breast Cancer in Shanghai, Fudan University Shanghai Cancer Center, Shanghai, China.

Background: To date, breast cancer remains the primary cause of tumor-related death among women, even though some leap-type developments of oncology have been done to slash the mortality. Considering the tumor heterogeneity and individual variation, the more reliable biomarkers are required to be identified for supporting the development of precision medicine in breast cancer.

Methods: Based on the TCGA-BRCA and METABRIC databases, the differently expressed RNA binding proteins (RBPs) between tumor and normal tissues were investigated. In this study, we focused on the communal differently expressed RBPs in four subtypes of breast cancer. Lasso-penalized Cox analysis, Stepwise-multivariate Cox analysis and Kaplan-Meier survival curve were performed to identify the hub RBP-coding genes in predicting prognosis of breast cancer, and a prognostic model was established. The efficiency of this model was further validated in other independent GSE20685, GSE4922 and FUSCC-TNBC cohorts by calculating the risk score and performing survival analysis, ROC and nomogram. Moreover, pathologic functions of the candidate RBPs in breast cancer were explored using some routine experiments , and the potential compounds targeting these RBPs were predicted by reviewing the Comparative Toxicogenomics Database.

Results: Here, we identified 62 RBPs which were differently expressed between the tumor and normal tissues. Thereinto, three RBPs (MRPL12, MRPL13 and POP1) acted as independent risk factors, and their expression pattern also correlated with poor prognosis of patients. A prognostic model, built with these 3-RBPs, possessed statistical significance to predict the survival probability of patients with breast cancer. Furthermore, experimental validations showed that down-regulating the expression of endogenous MRPL12, MRPL13 or POP1 could dramatically suppress the cellular viability and migration of breast cancer cells . Besides, some compounds (such as the Acetaminophen, Urethane and Tunicamycin) were predicted for curing breast cancer targeting MRPL12, MRPL13 and POP1 simultaneously.

Conclusion: This study identified and established a 3-RBPs-based signature and nomogram for predicting the survival probability of patients with breast cancer. MRPL12, MRPL13 and POP1 might act as oncogenes in maintaining cellular viability and accelerating metastasis of breast cancer cells, implying the possibility of which to be designed as biomarkers and/or therapeutic targets for breast cancer.
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http://dx.doi.org/10.3389/fonc.2021.663556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311660PMC
July 2021

Anti-aging properties of phytoconstituents and phyto-nanoemulsions and their application in managing aging-related diseases.

Adv Drug Deliv Rev 2021 09 24;176:113886. Epub 2021 Jul 24.

School of Science, RMIT University, Melbourne, VIC 3083, Australia. Electronic address:

Aging is spontaneous and inevitable process in all living beings. It is a complex natural phenomenon that manifests as a gradual decline of physiological functions and homeostasis. Aging inevitably leads to age-associated injuries, diseases, and eventually death. The research on aging-associated diseases aimed at delaying, preventing or even reversing the aging process are of great significance for healthy aging and also for scientific progress. Numerous plant-derived compounds have anti-aging effects, but their therapeutic potential is limited due to their short shelf-life and low bioavailability. As the novel delivery system, nanoemulsion can effectively improve this defect. Nanoemulsions enhance the delivery of drugs to the target site, maintain the plasma concentration for a longer period, and minimize adverse reaction and side effects. This review describes the importance of nanoemulsions for the delivery of phyto-derived compounds and highlights the importance of nanoemulsions in the treatment of aging-related diseases. It also covers the methods of preparation, fate and safety of nanoemulsions, which will provide valuable information for the development of new strategies in treatment of aging-related diseases.
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http://dx.doi.org/10.1016/j.addr.2021.113886DOI Listing
September 2021
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