Publications by authors named "Xu-dong Guo"

20 Publications

  • Page 1 of 1

Research progress on the regulatory role of microRNAs in spinal cord injury.

Regen Med 2021 05 6;16(5):465-476. Epub 2021 May 6.

The Second Clinical Medical College, Lanzhou University, Lanzhou, Gansu 730000, PR China.

Spinal cord injury (SCI) is a severe CNS injury that results in abnormalities in, or loss of, motor, sensory and autonomic nervous function. miRNAs belong to a new class of noncoding RNA that regulates the production of proteins and biological function of cells by silencing translation or interfering with the expression of target mRNAs. Following SCI, miRNAs related to oxidative stress, inflammation, autophagy, apoptosis and many other secondary injuries are differentially expressed, and these miRNAs play an important role in the progression of secondary injuries after SCI. The purpose of this review is to elucidate the differential expression and functional roles of miRNAs after SCI, thus providing references for further research on miRNAs in SCI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2217/rme-2020-0125DOI Listing
May 2021

Different Types of Double-Level Degenerative Lumber Spondylolisthesis: What Is Different in the Sagittal Plane?

World Neurosurg 2021 06 6;150:e127-e134. Epub 2021 Mar 6.

The Second Clinical Medical College, Lanzhou University, Lanzhou, Gansu, China; Department of Orthopedics, Lanzhou University Second Hospital, Lanzhou, Gansu, China; The International Cooperation Base of Gansu Province for the Pain Research in Spinal Disorders, Lanzhou, Gansu, China. Electronic address:

Background: Degenerative lumber spondylolisthesis (DLS) is a common orthopedic condition, described as a condition that compared with the lower vertebra, the superior vertebra slides forward or backward in the sagittal plane without accompanying isthmic spondylolisthesis. Information pertaining to different types of double-level DLS is scarce. This study aims to analyze parameters of patients with different types of double-level DLS to provide a reference for guiding surgical treatment and restoring sagittal balance of patients with DLS.

Methods: From January 2014 to January 2020, records of patients with double-level DLS were retrospectively reviewed. Patients with double-level DLS were divided into 3 types: anterior, posterior, and combined; the anterior and combined types were studied. The sagittal spinopelvic parameters included C7 tilt, maximal thoracic kyphosis, maximal lumbar lordosis (LLmax), pelvic incidence (PI), pelvic tilt (PT), and sacral slope (SS). After descriptive analysis, demographic and radiographic data were compared.

Results: Forty and 18 patients were included in the anterior and combined type groups, respectively. Both groups had different levels of chronic low back pain, but the incidence of radiating leg pain and neurogenic claudication was significantly higher in the anterior type. Oswestry Disability Index and visual analog scale low back scores were also higher in the anterior type. In the anterior type, C7 tilt (7.14 ± 2.15 vs. 5.41 ± 2.28, P = 0.007), LLmax (50.02 ± 14.76 vs. 36.96 ± 14.56, P = 0.003), PI (68.28 ± 9.16 vs. 55.53 ± 14.19, P < 0.001), PT (28.68 ± 7.31 vs. 19.38 ± 4.70, P < 0.001), and PT/PI (42.45 ± 11.22 vs. 36.04 ± 9.87, P = 0.041) were significantly higher. In the anterior type, PI correlated positively with LLmax (r = 0.59) and SS (r = 0.71). LLmax and SS (r = 0.65) had a positive correlation. PT/PI and SS (r = -0.77) had a negative correlation. In the combined type, PI correlated positively with LLmax (r = 0.61) and SS (r = 0.88), and PT/PI correlated negatively with SS (r = -0.81).

Conclusions: In patients with double-level DLS, the sagittal spinopelvic parameters differed between the anterior and combined types. Overall, spinal surgeons should focus on correcting sagittal deformities, relieving postoperative clinical symptoms, and improving quality of life during fusion surgery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.wneu.2021.02.125DOI Listing
June 2021

FTY720 Exerts Anti-Glioma Effects by Regulating the Glioma Microenvironment Through Increased CXCR4 Internalization by Glioma-Associated Microglia.

Front Immunol 2020 4;11:178. Epub 2020 Mar 4.

Neuroprotective Drug Discovery Key Laboratory of Nanjing Medical University, Jiangsu Key Laboratory of Neurodegeneration, Nanjing Medical University, Nanjing, China.

Glioblastoma (GBM) is one of the most malignant and aggressive primary brain tumors. The incurability of glioblastoma is heavily influenced by the glioma microenvironment. FTY720, a potent immunosuppressant, has been reported to exert anti-tumor effects in glioblastoma. However, the impact of FTY720 on the glioma microenvironment remains unclear. We examined the effects of FTY720 on the distribution and polarization of glioma-associated microglia and macrophages (GAMs) in glioma-bearing rats using immunofluorescence staining. qRT-PCR and Western blotting were used to detect the expressions of CXCR4 and MAPK pathway-related signal molecules on microglia in the coculture system. The levels of inflammatory factors were tested via ELISA. Wound healing assay and Matrigel invasion assay were used to determine the migration and invasion of C6 glioma cells. We discovered that FTY720 could inhibit the growth, migration, and invasion of glioma by targeting GAMs to impede their effect on glioma cells. Simultaneously, FTY720 could block the chemoattraction of GAMs by inhibiting MAPK-mediated secretion of IL-6 through increased internalization of CXCR4. Moreover, microglia and macrophages are polarized from pro-glioma to an anti-tumor phenotype. These results provide novel insights into the inhibitory effects of FTY720 on glioma by targeting GAMs-glioma interaction in the tumor microenvironment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2020.00178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065571PMC
February 2021

The Overexpression of Tβ4 in the Hair Follicle Tissue of Alpas Cashmere Goats Increases Cashmere Yield and Promotes Hair Follicle Development.

Animals (Basel) 2019 Dec 31;10(1). Epub 2019 Dec 31.

State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.

Increased cashmere yield and improved quality are some goals of cashmere goat breeding. Thymosin beta-4 (Tβ4) plays a key role in the growth and development of hair follicles. For the past ten years, we have evaluated the role of Tβ4 by establishing a flock of 15 cashmere goats that specifically overexpress the gene in the hair follicles. These Tβ4 overexpression (Tβ4-OE) cashmere goats had more secondary hair follicles than the WT goats and produced more cashmere. Meanwhile, combined analysis of the skin transcriptome and proteome in cashmere goats suggested that Tβ4 may affect hair growth by interacting with keratin type II cytoskeletal 4 epidermal (KRT4) to mediate the extracellular signal-regulated protein kinase (ERK) signaling pathway, thereby promoting the development of secondary hair follicles, and consequently, increasing cashmere yield. Thus, the specific overexpression of Tβ4 in the hair follicles of cashmere goats effectively increased the cashmere yield.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ani10010075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022706PMC
December 2019

[Moderate grazing increases the abundance of soil methane-oxidizing bacteria and CH uptake rate in a typical steppe of Inner Mongolia, China.]

Ying Yong Sheng Tai Xue Bao 2019 Jun;30(6):1919-1926

Ministry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau, Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China.

Microbial oxidation is the only biological sink of atmospheric methane (CH). It is essential to understand the variation of CH fluxes among different grassland use types for developing low-emission management system. Here, we measured the CH flux and the soil methane-oxidizing bacteria abundance in a typical steppe under grazing, mowing and fencing management in central Inner Mongolia, with the aims to determine the effects of these grassland use types on CH flux, and to test the hypothesis that pmoA functional gene abundance regulates CH fluxes. The measurements were conducted on the experimental grassland that had experienced four grassland use treatments over five years. The treatments were whole growing season grazing from May to September (T), spring and summer grazing (twice in May and July)(T), autumn mowing (T) and enclosure (T). We measured CH flux using static chamber method, and quantified the abundance of pmoA functional genes using molecular techniques. Moreover, we measured plant biomass and soil physicochemical properties. The results showed that moderate grazing significantly enhanced CH uptake rate and the methane-oxidizing bacteria abundance (i.e., the pmoA gene copy number per gram of dry soil). The pmoA gene copy number ranged from 6.9×10 to 3.9×10 per gram of dry soil in growing season. The CH uptake rate was (68.21±3.01) μg·m·h under T, which was 22.1%, 37.5% and 30.9% higher than that under T, T or T , respectively. The CH uptake rate was positively correlated with abundance of CH oxidizing bacteria and soil sand content, but negatively correlated with soil silt content, soil moisture, NH-N and NO-N content, and plant biomass. These results suggested that the steppe ecosystem is a CH sink under all land-use types in central Inner Mongolia, and that moderate grazing would enhance methane-oxidizing bacteria abundance and CH uptake by improving soil sand content, reducing soil mineral nitrogen content and plant production in the typical steppe ecosystem. These results were of significance for the development of low-emission grassland management system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13287/j.1001-9332.201906.035DOI Listing
June 2019

Establishment and evaluation of a novel mouse model of peri/postmenopausal depression.

Heliyon 2019 Feb 4;5(2):e01195. Epub 2019 Feb 4.

Neuroprotective Drug Discovery Key Laboratory of Nanjing Medical University, Nanjing, Jiangsu, 211166, China.

Women are believed to be more vulnerable to develop depressive symptoms during the perimenopause compared to postmenopause. The traditional bilateral ovariectomy and chronic mild stress (CMS) stimulation animal model produces a postmenopausal depressive-like state but the transition from perimenopausal period to postmenopausal period was ignored. Thus we establish a novel animal model in which the mice were stimulated by CMS for three months and removed the ovaries by two-step operation, and then evaluate whether this novel model could be much better for preclinical study used as a peri/postmenopause depressive model. The present study systemically evaluated the changes induced by two-step ovariectomy plus CMS in the mice. The depression-like behaviors, the levels of corticosterone, estrogen, pro-inflammatory factors, neurotransmitters, as well as brain-derived neurotrophic factor were determined; the changes of estrogen receptors, serotonin receptors, uterine weight and bone microarchitecture were also observed. The results show that the behaviors and biochemical indexes of mice changed gradually over time. Our study suggests that this two-step ovariectomy operation plus CMS successfully establishes a more reasonable peri/postmenopausal depression animal model which effectively simulates the clinical symptoms of peri/postmenopausal depressive women.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.heliyon.2019.e01195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365542PMC
February 2019

[The effect of cgVEGF164 on the growth of murine hair follicles].

Yi Chuan 2019 Jan;41(1):76-84

State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010070, China.

Vascular endothelial growth factor (VEGF) is a dimeric glycoprotein that induces proliferation and migration of vascular endothelial cells as well as regulation of capillary formation around hair follicles which affects the growth and development of hair follicles. cgVEGF164 is a major splice variant of the cashmere goat VEGF-A gene, but its regulation on hair follicles is rarely known. In order to investigate the role of cgVEGF164 on the growth of murine hair follicles, we produced keratin 14 promoter-driven cgVEGF164 transgenic mice via pronuclear microinjection. Firstly, the diameter and density of hair follicles of transgenic mice were compared with non-transgenic control mice in paraffin sections stained by hematoxylin-eosin (H&E). Then, protein expression levels and the phosphorylation of ERK1/2, AKT1 and LEF1 were examined by Western blot. There are five positive individuals among the neonatal mice (positive rate is 8.5%). Compared with non-transgenic control mice, the diameter and density of hair follicles in transgenic mice are both obviously increased. The expression levels of P-ERK1/2/ERK1/2, P-AKT1/AKT1 and P-LEF1/LEF1 are significantly higher in transgenic mice than those in non-transgenic control mice. Based on these results, we conclude that cgVEGF164 as a growth factor can improve the growth of hair follicles which might be mediated by increasing the levels of ERK1/2, AKT1, and LEF1 protein phosphorylation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.16288/j.yczz.18-136DOI Listing
January 2019

Antagonizing peroxisome proliferator-activated receptor γ facilitates M1-to-M2 shift of microglia by enhancing autophagy via the LKB1-AMPK signaling pathway.

Aging Cell 2018 08 8;17(4):e12774. Epub 2018 May 8.

Neuroprotective Drug Discovery Key Laboratory of Nanjing Medical University, Nanjing, Jiangsu, China.

Microglia-mediated neuroinflammation plays a dual role in various brain diseases due to distinct microglial phenotypes, including deleterious M1 and neuroprotective M2. There is growing evidence that the peroxisome proliferator-activated receptor γ (PPARγ) agonist rosiglitazone prevents lipopolysaccharide (LPS)-induced microglial activation. Here, we observed that antagonizing PPARγ promoted LPS-stimulated changes in polarization from the M1 to the M2 phenotype in primary microglia. PPARγ antagonist T0070907 increased the expression of M2 markers, including CD206, IL-4, IGF-1, TGF-β1, TGF-β2, TGF-β3, G-CSF, and GM-CSF, and reduced the expression of M1 markers, such as CD86, Cox-2, iNOS, IL-1β, IL-6, TNF-α, IFN-γ, and CCL2, thereby inhibiting NFκB-IKKβ activation. Moreover, antagonizing PPARγ promoted microglial autophagy, as indicated by the downregulation of P62 and the upregulation of Beclin1, Atg5, and LC3-II/LC3-I, thereby enhancing the formation of autophagosomes and their degradation by lysosomes in microglia. Furthermore, we found that an increase in LKB1-STRAD-MO25 complex formation enhances autophagy. The LKB1 inhibitor radicicol or knocking down LKB1 prevented autophagy improvement and the M1-to-M2 phenotype shift by T0070907. Simultaneously, we found that knocking down PPARγ in BV2 microglial cells also activated LKB1-AMPK signaling and inhibited NFκB-IKKβ activation, which are similar to the effects of antagonizing PPARγ. Taken together, our findings demonstrate that antagonizing PPARγ promotes the M1-to-M2 phenotypic shift in LPS-induced microglia, which might be due to improved autophagy via the activation of the LKB1-AMPK signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/acel.12774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052482PMC
August 2018

Construction of FGF21 knockout mouse models by the CRISPR/Cas9 system.

Yi Chuan 2018 Jan;40(1):66-74

The Research Center for Laboratory Animal Sciense, College of Life Science, Inner Mongolia University, Hohhot 010070, China.

Fibroblast growth factors (FGFs) are multifunctional signal molecules between cells, regulating the various physiological functions of the organism. FGF21 is a regulatory factor of the FGF family and has been postulated to play important roles in hair follicle development and hair follicle growth cycle. To evaluate the roles of FGF21, we had established a FGF21 knockout mouse model, using the CRISPR/Cas9 technology. We had constructed a FGF21 targeting vector and microinjected it with Cas9 mRNA and gRNA into fertilized ova of FVB mice. The gRNA was designed to target the exon 1 of the endogenous mouse FGF21 gene. Three lines of Fgf21 mice were obtained from these experiments, and confirmed to harbor Fgf21 genotypes and null expression phenotype, using DNA sequencing, qRT-PCR and Western blotting. FGF21 mRNA and FGF21 protein were not detected in tissues of these Fgf21 mice. Depilation and histochemistry analyses showed that the Fgf21 mice had lower body weight, slower hair regrowth and poorer hair quantities and smaller hair follicles diameters, as compared to WT mice. The Fgf21 mice reported here could provide a useful genetic model for future studies of FGF21 functions in hair follicle development and hair follicle growth cycle.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.16288/j.yczz.17-011DOI Listing
January 2018

PD149163 induces hypothermia to protect against brain injury in acute cerebral ischemic rats.

J Pharmacol Sci 2017 Nov 23;135(3):105-113. Epub 2017 Oct 23.

Neuroprotective Drug Discovery Key Laboratory, Department of Pharmacology, Nanjing Medical University, 101 Longmian Road, Nanjing, Jiangsu 211166, China. Electronic address:

Therapeutic hypothermia is a promising strategy for acute cerebral ischemia via physical or pharmacological methods. In this study, we pharmacologically induced hypothermia on Sprague Dawley rats by intraperitoneally injecting PD149163. We found that mild hypothermia was induced by PD149163 treatment without local cerebral blood flow (LCBF) alteration. To evaluate the neuroprotective effects of PD149163, TTC staining, HE staining and Nissl's staining were performed in our study. We found that PD149163 could prevent neuronal damage, and inhibit proliferation and activation of glial cells induced by ischemia. Simultaneously, we observed PD149163 ameliorated apoptosis characterized by down-regulated caspase-3 and Bax, but elevated Bcl-2. Moreover, PD149163 dramatically reduced JNK and AMPK/mTOR signaling pathway activation, and thereby inhibited autophagy by increased P62 expression, decreased the ratio of LC-Ⅱ to LC-Ⅰ and the expression of Beclin. Taken together, the present findings reveal the therapeutic effects of PD149163-induced hypothermia in brain ischemia, and provide a new strategy for stroke treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jphs.2017.10.004DOI Listing
November 2017

Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis.

Front Mol Neurosci 2017 14;10:293. Epub 2017 Sep 14.

Neuroprotective Drug Discovery Key Laboratory, Department of Pharmacology, Nanjing Medical UniversityNanjing, China.

There is increasing interest in the association between depression and the development of metabolic diseases. Rosiglitazone, a therapeutic drug used to treat type 2 diabetes mellitus, has shown neuroprotective effects in patients with stroke and Alzheimer's disease. The present study was performed to evaluate the possible roles of rosiglitazone in (unpredictable chronic mild stress-induced depressive mouse model) and (corticosterone-induced cellular model) depressive models. The results showed that rosiglitazone reversed depressive behaviors in mice, as indicated by the forced swimming test and open field test. Rosiglitazone was also found to inhibit the inflammatory response, decrease corticosterone levels, and promote astrocyte proliferation and neuronal axon plasticity in the prefrontal cortex of mice. This series of and experiments showed that autophagy among neurons was inhibited in depressive models and that rosiglitazone promoted autophagy by upregulating LKB1, which exerted neuroprotective effects. Rosiglitazone was also found to activate the Akt/CREB pathway by increasing IGF-1R expression and IGF-1 protein levels, thereby playing an anti-apoptotic role in astrocytes. Rosiglitazone's autophagy promotion and neuroprotective effects were found to be reversed by the PPARγ antagonist T0070907 in primary neurons and by PPARγ knockdown in an N2a cell line. In conclusion, we found that rosiglitazone protects both neurons and astrocytes in and depressive models, thereby playing an anti-depressive role. These findings suggest that PPARγ could be a new target in the development of anti-depressive drugs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnmol.2017.00293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603714PMC
September 2017

A New Vegetation Index Infusing Visible-Infrared Spectral Absorption Feature for Natural Grassland FAPAR Retrieval.

Guang Pu Xue Yu Guang Pu Fen Xi 2017 Mar;37(3):859-64

Considering the close relationship between spectral absorption features of visible-near infrared and “Fraction of Absorbed Photosynthetically Active Radiation(FAPAR)”, the “automatic recognition method of hyperspectral curve’s characteristic absorption peak” and “quantization method of spectral absorption characteristic parameters” were used to extract the hyperspectral absorption characteristic parameters which are sensitive to FAPAR. Referring to mathematical form of vegetation index, visible-near infrared spectral absorption characteristic parameters were used to replace spectral reflectance and create a new vegetation index to estimate FAPAR of vegetation. The data from 2014 and 2015 on typical natural grassland community canopy in the middle and eastern Inner Mongolia was chosen to build and verify the model of estimating FAPAR. The results showed that new vegetation index “SAI-VI” effectively raised the FAPAR estimating accuracy in the middle and low vegetation coverage areas. Compared with other seven different types of visible-near infrared vegetation index, it has a higher correlation with the value of FAPAR(the largest correlation coefficient is 0.801). The FAPAR prediction index model which takes “SAI-VI” as variable has higher precision and better stability(the determination coefficients of modeling and testing are the highest and both are above 0.75, the “Root Mean Square Error (RMSE)” and “Average Error Coefficient (MEC)” are the minimum). The research also showed that the new vegetation index “SAI-VI” infusing visible-infrared spectral absorption feature highlights the difference between visible spectral and near infrared spectral absorption characteristic parameters. While comparing with single spectral absorption characteristic parameter, “SAI-VI” can depress the influence of soil and enhance the sensitivity to the changes of FAPAR. “SAI-VI” also included the information of hyperspectral absorption characteristic parameters which are sensitive to FAPAR and expressed more detailed information of FAPAR while comparing with original spectral reflectance. “SAI-VI” can be used as a new parameter in inversion of vegetation canopy FAPAR, to some extent it could remedy defect of vegetation index method in estimating FAPAR.
View Article and Find Full Text PDF

Download full-text PDF

Source
March 2017

[Vegetation carbon stocks and net primary productivity of the mangrove forests in Shenzhen, China.]

Ying Yong Sheng Tai Xue Bao 2016 Jul;27(7):2059-2065

Ministry of Education Key Laboratory for Coastal and Wetland Ecosystems/College of the Environment and Ecology, Xiamen University, Xiamen 361102, Fujian, China.

Mangroves are the most important coastal blue carbon sinks. The accurate estimation on the carbon sequestration capacity of plant communities would guide the mangrove conservation, afforestation and management. This study investigated the vegetation carbon stocks of dominant mangrove communities, which were Avicennia marina, Kandelia obovata, Sonneratia caseolaris, and Sonneratia apetala in Futian Nature Mangrove Reserve in Shenzhen, Guangdong Province of China. Vegetation carbon stock consisted of living trees (aboveground and belowground biomass), understory, pneumatophore, standing dead trees, fallen dead trees and litter in these communities. The net primary productivity (NPP) was calculated from the litterfall and incremental growth in the same year of each community. Our results showed that the vegetation carbon stocks for A. marina, K. obovata, S. caseolaris, and S. apetala communities were 28.7, 127.6, 100.1, and 73.6 t C·hm, and the NPP were 8.75, 7.67, 9.60, and 11.8 t C·hm·a, respectively. Therefore, acting as urban forests, Futian mangroves in Shenzhen assimilated about 4000 t CO·a. These results provided guidance for mangrove blue carbon assessment, and theoretical basis for the construction of coastal carbon sequestration forests in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13287/j.1001-9332.201607.029DOI Listing
July 2016

Repetitive magnetic stimulation affects the microenvironment of nerve regeneration and evoked potentials after spinal cord injury.

Neural Regen Res 2016 May;11(5):816-22

Department of Burns and Plastic Surgery, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China.

Repetitive magnetic stimulation has been shown to alter local blood flow of the brain, excite the corticospinal tract and muscle, and induce motor function recovery. We established a rat model of acute spinal cord injury using the modified Allen's method. After 4 hours of injury, rat models received repetitive magnetic stimulation, with a stimulus intensity of 35% maximum output intensity, 5-Hz frequency, 5 seconds for each sequence, and an interval of 2 minutes. This was repeated for a total of 10 sequences, once a day, 5 days in a week, for 2 consecutive weeks. After repetitive magnetic stimulation, the number of apoptotic cells decreased, matrix metalloproteinase 9/2 gene and protein expression decreased, nestin expression increased, somatosensory and motor-evoked potentials recovered, and motor function recovered in the injured spinal cord. These findings confirm that repetitive magnetic stimulation of the spinal cord improved the microenvironment of neural regeneration, reduced neuronal apoptosis, and induced neuroprotective and repair effects on the injured spinal cord.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/1673-5374.182710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4904474PMC
May 2016

Role of thymosin beta 4 in hair growth.

Mol Genet Genomics 2016 Aug 29;291(4):1639-46. Epub 2016 Apr 29.

National Research Center for Animal Transgenic Biotechnology, Inner Mongolia University, Hohhot, 010070, China.

Although thymosin beta 4 (Tβ4) is known to play a role in hair growth, its mechanism of action is unclear. We examined the levels of key genes in a Tβ4 epidermal-specific over-expressing mouse model and Tβ4 global knockout mouse model to explore how Tβ4 affects hair growth. By depilation and histological examination of the skin, we confirmed the effect of Tβ4 on hair growth, the number of hair shafts and hair follicle (HF) structure. The mRNA and protein expression of several genes involved in hair growth were detected by real-time PCR and western blotting, respectively. Changes in the expression of β-catenin and Lef-1, the two key molecules in the Wnt signaling pathway, were similar to the changes observed in Tβ4 expression. We also found that compared to the control mice, the mRNA and protein expression of MMP-2 and VEGF were increased in the Tβ4 over-expressing mice, while the level of E-cadherin (E-cad) remained the same. Further, in the Tβ4 global knockout mice, the mRNA and protein levels of MMP-2 and VEGF decreased dramatically and the level of E-cad was stable. Based on the above results, we believe that Tβ4 may regulate the levels of VEGF and MMP-2 via the Wnt/β-catenin/Lef-1 signaling pathway to influence the growth of blood vessels around HFs and to activate cell migration. Tβ4 may have potential for the treatment of hair growth problems in adults, and its effects should be further confirmed in future studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00438-016-1207-yDOI Listing
August 2016

A retrospective study of laparoscopic unilateral adrenalectomy for primary hyperaldosteronism caused by unilateral adrenal hyperplasia.

Int Urol Nephrol 2014 Jul 2;46(7):1283-8. Epub 2014 Feb 2.

Minimally Invasive Urology Center, East District of Provincial Hospital Affiliated to Shandong University, 9th Floor, Block C, No. 9677, East Jingshi Road, Jinan, 250014, People's Republic of China.

Objective: To evaluated the long-term outcomes of laparoscopic unilateral adrenalectomy for primary aldosteronism (PA) caused by unilateral adrenal hyperplasia (UAH).

Methods: One hundred and sixty-four patients who underwent laparoscopic unilateral adrenalectomy for UAH from January 2004 to December 2011 were entered in this retrospective analysis. Patients demographics, perioperative parameters, and follow-up results were recorded and analyzed statistically.

Results: All 164 cases suffered hypertension with biochemical evidence of hyperaldosteronism prior to operation. Hypokalemia was observed in 52/164 (37.14%) patients. UAH was proved by multi-slice computed tomography (MSCT). All operations were completed successfully without any conversions or complications. Postoperative pathology confirmed that 164 cases were cortical nodular hyperplasia, of which 4 cases coexist with medullary hyperplasia and 7 with micro-adenoma. At the median follow-up of 48 months, hypertension was cured in 88 (53.7%) patients, improved in 71 (43.3%) patients, and refractory in 5 (3.05%) patients. Hypokalemia and hyperaldosteronism were cured in all patients except re-elevation of blood pressure and plasma aldosterone in two patients 1 month after adrenalectomy.

Conclusions: As an underestimated subtype of PA, UAH is accepted gradually. Laparoscopic unilateral adrenalectomy is nowadays the preferred approach to treat patients with PA caused by UAH. When adrenal venous sampling is not allowed, high-resolution MSCT is a reliable test for lateralization of aldosterone hypersecretion in carefully selected patients and 97% had either cure or improvement in blood pressure control.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11255-013-0614-9DOI Listing
July 2014

Reconstruction and visualization of human gastrointestinal tract.

Int J Biomed Sci 2012 Mar;8(1):22-7

School of medical instrument and food engineering, University of Shanghai for Science and Technology, Shanghai, China;

Background: Converting the two-dimensional (2D) cross-sectional photographs into an intuitive three-dimensional (3D) model is a basic task for medical imaging data for auxiliary disease-linked diagnosis purpose.

Methods: Reconstruction and visualization process of gastrointestinal cross-sectional photographs includes image preparation, image registration, image segmentation, 3D surface-rendering reconstruction, and implementation of 3D digital visualization.

Results: Using the visualization toolkit (VTK), we implemented 3D digital reconstruction and visualization of gastrointestinal tract, whose visualized model can be zoomed, paned, and rotated, including the stomach, the small intestine, and the large intestine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614852PMC
March 2012

[Multi-scale ecological security evaluation of typical fragile areas in Loess Plateau].

Ying Yong Sheng Tai Xue Bao 2007 Jul;18(7):1554-9

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China.

Regional ecological security has been paid more and more attention, and it's of special significance to study the ecological security and its driving factors in ecological fragile areas. In this paper, the ecological security and its driving factors in typical fragile regions in Loess Plateau were analyzed at district and county scales. Yulin District and Hengshan County were taken as the cases, and ecological security index system was constructed to assess the security levels of the two regions. By using AHP method, the spatial differentiation of ecological security at different scales was studied, and the results showed that there existed differences among the twelve counties in Yulin District, with the security level being generally higher in southwest and lower in northeast. Similar tendency was observed in Hengshan County, with the security level being lower in north and northeast, and varying among different scales in southwest. Climate and topography were the weak driving forces, while human disturbances had great influence on ecological security.
View Article and Find Full Text PDF

Download full-text PDF

Source
July 2007

[A rapid method for preparation of plasmid DNA for screening recombinant clones].

Sheng Wu Gong Cheng Xue Bao 2007 Jan;23(1):176-8

The Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot 010021, China.

A simple and rapid method for preparation of plasmid DNA from overnight incubation was introduced. It does not require any additional reagents; the incubation mixture containing recombinant plasmid DNA was just mixed with H2O and phenol/chloroform/isoamyl alcohol in certain ratio. After vortexing and spinning of the mixture, the supernatant could be directly loaded onto agarose gel and analyzed using electrophoresis. The whole preparation requires only 3-5 minutes. So to quickly screen recombinant clones, this method is better compared with traditional methods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/s1872-2075(07)60015-6DOI Listing
January 2007

[Optimization of parameters of exogene transfection of bovine fetal fibroblasts in vitro mediated by liposome].

Yi Chuan 2002 Nov;24(6):653-5

The Research Center for Laboratory Animal Science of Inner Mongolia University, The Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology, Huhhot 010021, China.

pEGFP-C1 eucaryon expression vector was successfully transfected by liposome into bovine fetal fibroblasts. We investigated the effect of parameter such as the dose of DNA and liposome,number of cell transfected and exposure time of the cell to the DNA-liposome complexes. It was indicated that GFP (green fluorescent protein) expression was enhanced as the dose of DNA and liposome increased and on decline as the exposure time was prolonged. The improvement of transfection efficiency depent on the suitable cell number.
View Article and Find Full Text PDF

Download full-text PDF

Source
November 2002
-->