Publications by authors named "Xu Zhang"

3,651 Publications

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MAT2: Manifold alignment of single-cell transcriptomes with cell triplets.

Bioinformatics 2021 May 10. Epub 2021 May 10.

Institute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai 200433, China.

Motivation: Aligning single-cell transcriptomes is important for the joint analysis of multiple single-cell RNA sequencing datasets, which in turn is vital to establishing a holistic cellular landscape of certain biological processes. Although numbers of approaches have been proposed for this problem, most of which only consider mutual neighbors when aligning the cells without taking into account known cell type annotations.

Results: In this work, we present MAT2 that aligns cells in the manifold space with a deep neural network employing contrastive learning strategy. Compared with other manifold-based approaches, MAT2 has two-fold advantages. Firstly, with cell triplets defined based on known cell type annotations, the consensus manifold yielded by the alignment procedure is more robust especially for datasets with limited common cell types. Secondly, the batch-effect-free gene expression reconstructed by MAT2 can better help annotate cell types. Benchmarking results on real scRNA-seq datasets demonstrate that MAT2 outperforms existing popular methods. Moreover, with MAT2, the hematopoietic stem cells are found to differentiate at different paces between human and mouse.

Availability And Implementation: MAT2 is publicly available at https://github.com/Zhang-Jinglong/MAT2.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btab250DOI Listing
May 2021

MAT2: Manifold alignment of single-cell transcriptomes with cell triplets.

Bioinformatics 2021 May 11. Epub 2021 May 11.

Institute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai 200433, China.

Motivation: Aligning single-cell transcriptomes is important for the joint analysis of multiple single-cell RNA sequencing datasets, which in turn is vital to establishing a holistic cellular landscape of certain biological processes. Although numbers of approaches have been proposed for this problem, most of which only consider mutual neighbors when aligning the cells without taking into account known cell type annotations.

Results: In this work, we present MAT2 that aligns cells in the manifold space with a deep neural network employing contrastive learning strategy. Compared with other manifold-based approaches, MAT2 has two-fold advantages. Firstly, with cell triplets defined based on known cell type annotations, the consensus manifold yielded by the alignment procedure is more robust especially for datasets with limited common cell types. Secondly, the batch-effect-free gene expression reconstructed by MAT2 can better help annotate cell types. Benchmarking results on real scRNA-seq datasets demonstrate that MAT2 outperforms existing popular methods. Moreover, with MAT2, the hematopoietic stem cells are found to differentiate at different paces between human and mouse.

Availability And Implementation: MAT2 is publicly available at https://github.com/Zhang-Jinglong/MAT2.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btab260DOI Listing
May 2021

The Clinical Accuracy and Risk Stratification in End of Therapy F-FDG PET/CT in Burkitt Lymphoma.

Front Oncol 2021 21;11:625436. Epub 2021 Apr 21.

Sun Yat-sen University Cancer Center (SYSUCC), Guangzhou, China.

Purpose: Burkitt lymphoma (BL) is an invasive lymphoma subtype with FDG avid at F-FDG PET/CT, but there is currently no validated criterion in treatment evaluation and prognosis prediction. The aim of this study was to analyze the clinical accuracy of F-FDG PET/CT in Burkitt lymphoma in end of therapy PET/CT (EOT-PET) to assess the treatment response in BL and conduct a survival analysis with different Deauville 5-point score (DS) cutoff values.

Materials And Methods: A total of 189 patients were retrospectively included: 97 underwent baseline PET/CT and all underwent EOT-PET. Survival curves were plotted according to the Kaplan-Meier method. Different DS cutoff values in EOT-PET were evaluated for risk stratification in Burkitt lymphoma.

Results: The median progression free survival (PFS) and overall survival (OS) were 52 and 53 months, respectively. Applying the conventional DS 4 to 5, there was significant difference in outcome between EOT-PET negative and positive patients. However, the positive predictive value (PPV) (28.3% for PFS and 26.4% for OS) is low despite a high negative predictive value (NPV) (94.1% for OS and 94.9% for OS). When we moved the cutoff point to DS 5, the PPV was improved evidently (88.2% for PFS and 82.3% for OS) with the satisfactory NPV simultaneously (95.3% for PFS and 95.9% for OS).

Conclusions: EOT-PET results using DS significantly related with PFS and OS. DS of 5 may be a better cutoff point at the end of treatment to determine whether patients have a significant risk of recurrence or progress.
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http://dx.doi.org/10.3389/fonc.2021.625436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8098980PMC
April 2021

[Pleurodesis with an Autologous Blood Patch in the Treatment of Persistent Air Leak after Lung Resection].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2021 Apr;43(2):211-215

Department of Thoracic Surgery,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China.

Objective To evaluate the efficacy and risks of autologous blood patch pleurodesis in patients with persistent air leak(PAL)after lung resection. Methods A total of 97 patients with PAL after lung resection in Beijing Shijitan Hospital from October 2014 to October 2019 were retrospectively reviewed,including 53 treated by autologous blood patch pleurodesis and 44 by the conventional way.The therapeutic effect,adverse reactions and complications were analyzed. Results All the patients with PAL were cured with autologous blood patch pleurodesis.Most air leaks(81.1%)ceased within 48 hours after treatment,and the left 18.9% patients got cured after a repeat.The mean tube retention time and the mean in-hospital stay were 8.4 days and 10.0 days in the autologous blood patch pleurodesis group and 13.5 days and 15.3 days in the conventional treatment group.A prolonged drainage time(P=0.00)and in-hospital stay(P=0.00)were observed in the conventional treatment group.No severe complications were observed except two patients developed slight fever and cutaneous emphysema. Conclusion In our experience,the autologous blood patch pleurodesis is an effective way with low risk of adverse reactions in the treatment of PAL.
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http://dx.doi.org/10.3881/j.issn.1000-503X.12784DOI Listing
April 2021

Knowledge, attitude, and practice of obesity among university students.

Ann Palliat Med 2021 Apr;10(4):4539-4546

School of Public Health and Management, Binzhou Medical University, Yantai, China.

Background: Obesity, as an epidemic disease, is distributed among all age groups, including children, adolescents, adults, and the elderly. The goal of this study was to investigate the knowledge, attitude, and practice (KAP) concerning obesity among university students.

Methods: In total, 1,317 questionnaires were filled out by 1,317 (466 male and 851 female) randomly selected students aged range from 16 to 24. All participants agreed to provide personal information in this study. A self-designed questionnaire was applied to collect demographic characteristics and assess the KAP of obesity. Gender, height, weight, grade, sleep and income were included in the population questionnaire. Descriptive statistics was used to analyze the respondent rate of KAP among students.

Results: In the survey, 64.9% of respondents believed that obesity was a disease. Only a few people cared about their body shape. Most of the participants in the study had practices that were detrimental to their health, including irregular dieting (52.9%), surfing the internet, and playing games (58.5%) in their spare time.

Conclusions: This study identified that lacking knowledge of obesity was common among college students. Most respondents had a positive attitude about preventing obesity by focusing on dieting and exercise. Thus, education related to obesity should be strengthening among university students to translate attitude into practice.
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http://dx.doi.org/10.21037/apm-21-573DOI Listing
April 2021

Ultra-short time imaging of total-body PET/CT for cancer pain-induced untenable body position.

Eur J Nucl Med Mol Imaging 2021 May 8. Epub 2021 May 8.

Department of Nuclear Medicine, Sun Yat-Sen University Cancer Center, Sun Yat-Sen University, Guangzhou, 510030, Guangdong, China.

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http://dx.doi.org/10.1007/s00259-021-05380-6DOI Listing
May 2021

A Combination of Atorvastatin and Aspirin Enhances the Pro-Regenerative Interactions of Marrow Stromal Cells and Stroke-Derived Monocytes .

Front Pharmacol 2021 20;12:589418. Epub 2021 Apr 20.

Department of Neurology, Institute for Stroke and Cerebrovascular Disease, McGovern Medical School at UTHealth, Houston, TX, United States.

Marrow stromal cells (MSCs) are being tested in clinical trials for stroke patients. MSCs appear to promote recovery through secretomes that promote modulation of immune cells, including myeloid phagocytes. Many stroke patients have comorbidities such as metabolic syndrome, hypertension, hypercholesterolemia, and diabetes for which they are prescribed medications that might affect the function of MSCs and monocytes (Mo) when they are administered in stroke patients. We studied the effects of the two most commonly prescribed stroke medications, statin and statin plus aspirin, on the secretomes of MSCs and their modulation of Mo derived from stroke patients. Human MSCs, Mo and their co-cultures were exposed to atorvastatin or atorvastatin plus aspirin followed by secretome analysis at 24 h. Monocytes were isolated from healthy controls as well as stroke patients with NIHSS ranging from 11 to 20. Secretome composition was measured using multiplex immunoassay. We used MTT assay to measure proliferation of monocytes. The mixed model was used to analyze experimental data. -values less than 0.05 were considered significant. Atorvastatin and aspirin combination increased the release of IL-1RA from stroke Mo. In MSCs, atorvastatin and aspirin combination reduced the release of pro-inflammatory cytokines such as IL-6, IL-8, MCP-1 and IFN-γ. Atorvastatin alone reduced the release of IL-6, IL-8 and MCP-1 from co-cultures of stroke monocytes and MSCs. Combination of atorvastatin and aspirin had additive effect on reducing the secretion of IL-6 from co-cultures of stroke Mo and MSCs. Atorvastatin, alone and in combination with aspirin can promote anti-inflammatory effect by modulating the secretome profile of Mo and MSCs. Our results suggest that stroke trials involving the use of intravenous MSCs should consider the effect of aspirin and atorvastatin, both of which are administered to the majority of hospitalized ischemic stroke patients.
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http://dx.doi.org/10.3389/fphar.2021.589418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093790PMC
April 2021

Protein Transnitrosylation Signaling Networks Contribute to Inflammaging and Neurodegenerative Disorders.

Antioxid Redox Signal 2021 May 6. Epub 2021 May 6.

Scripps Research Institute, 4356, 10550 N Torrey Pines Rd, Calibr-125, La Jolla, California, United States, 92037-1000;

Significance: Physiological concentrations of nitric oxide (NO•) and related reactive nitrogen species (RNS) mediate multiple signaling pathways in the nervous system. During inflammaging (chronic low-grade inflammation associated with aging) and in neurodegenerative diseases, excessive RNS contribute to synapse and neuronal loss. 'NO signaling' in both health and disease is largely mediated through protein S-nitrosylation, a redox-based posttranslational modification with 'NO' [possibly in the form of nitrosonium cation (NO+)] reacting with cysteine thiol [or, more properly, thiolate anion (R-S-)]. Recent Advances: Emerging evidence suggests that S-nitrosylation occurs predominantly via transnitros(yl)ation. Mechanistically, the reaction involves thiolate anion, as a nucleophile, performing a reversible nucleophilic attack on a nitroso nitrogen to form an SNO-protein adduct. Prior studies identified transnitrosylation reactions between GAPDH-nuclear proteins, thioredoxin-caspase-3, and XIAP-caspase-3. Recently, we discovered that enzymes previously thought to act in completely disparate biochemical pathways can transnitrosylate one another during inflammaging in an unexpected fashion to mediate neurodegeneration. Accordingly, we reported a concerted tri-component transnitrosylation network from Uch-L1-to-Cdk5-to-Drp1 that mediates synaptic damage in Alzheimer's disease.

Critical Issues: Transnitrosylation represents a critical chemical mechanism for transduction of redox-mediated events to distinct subsets of proteins. Although thousands of thiol-containing proteins undergo S-nitrosylation, how transnitrosylation regulates a myriad of neuronal attributes is just now being uncovered. In this review, we highlight recent progress in the study of the chemical biology of transnitrosylation between proteins as a mechanism of disease.

Future Directions: We discuss future areas of study of protein transnitrosylation that link our understanding of aging, inflammation, and neurodegenerative diseases.
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http://dx.doi.org/10.1089/ars.2021.0081DOI Listing
May 2021

δ-MnO changed the structure of humic-like acid during co-composting of chicken manure and rice straw.

Waste Manag 2021 May 3;128:16-24. Epub 2021 May 3.

College of Life Science, Northeast Agricultural University, Harbin 150030, China.

Improving the structure and quantity of humus is important to reduce agriculture organic waste by composting. The present study was aimed to assess the role of δ-MnO on humus fractions formation during co-composting of chicken manure and rice straw. Two tests (control group (CK), the addition of δ-MnO (M)) were carried out. The results showed that organic matter content decreased by 34% and 29% at M and CK, suggesting the process of organic waste disposal was accelerated by adding δ-MnO. The structures and quantity of fulvic acid (FA) and humic acid (HA) (as the main fractions of humus) were investigated. The δ-MnO had no significant effect on improving the concentration of FA and HA (p > 0.05). However, the addition of δ-MnO caused different effects on the FA and HA structure. The humification degree of FA improved, while bioavailability of HA increased through adding δ-MnO. The addition of δ-MnO rephased the bacterial community structure, slowing down the succession rate of the bacterial community in M composting. After adding δ-MnO the structural equation modeling results showed that environmental factors could directly drive changes in FA and HA by modulating the bacterial community. Furthermore, the role of FA and HA in the soil amendment was also demonstrated. Therefore, the addition of MnO might be promising for agriculture organic waste treatment and environmental repair during composting.
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http://dx.doi.org/10.1016/j.wasman.2021.04.039DOI Listing
May 2021

Facile Synthesis of Imidazolium-Based Block Copolypeptides with Excellent Antimicrobial Activity.

Biomacromolecules 2021 May 6. Epub 2021 May 6.

Key Laboratory of Polymeric Materials and Application Technology of Hunan Province, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.

Antimicrobial polypeptides are promising mimics of antimicrobial peptides (AMPs) with low risks of antimicrobial resistance (AMR). Polypeptides with facile and efficient production, high antimicrobial activity, and low toxicity toward mammalian cells are highly desirable for practical applications. Herein, triblock copolypeptides with chloro groups (PPG-PCPBLG) and different main-chain lengths were synthesized via an ultrafast ring-opening polymerization (ROP) using a macroinitiator, namely poly(propylene glycol) bis(2-aminopropyl ether), and purified or nonpurified monomer (i.e., CPBLG-NCA). PPG-PCPBLG with 90 amino acid residues can be readily prepared within 300 s. Imidazolium-based block copolypeptides (PPG-PIL) were facilely prepared via nucleophilic substitution of PPG-PCPBLG with NaN and subsequent "click" chemistry. α-Helical PPG-PIL can self-assemble into nanostructured and cationic micelles which displayed highly potent antimicrobial activity and low hemolysis. The top-performing material, namely PPG-PIL, showed low minimum inhibitory concentration (MIC) against both Gram-positive and Gram-negative (25 μg mL). It also displayed low toxicity against mouse embryonic fibroblast (NIH 3T3) and human embryonic kidney (293T) cells at 2× MIC.
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http://dx.doi.org/10.1021/acs.biomac.1c00126DOI Listing
May 2021

Nitrite-enhanced copper-based Fenton reactions for biofilm removal.

Chem Commun (Camb) 2021 May 6. Epub 2021 May 6.

Department of Chemistry, Cape Breton University, Sydney, Nova Scotia B1P 6L2, Canada.

Unwanted biofilms present challenges for many industries. Herein an innovative biofilm removal technology was developed based on nitrite-accelerated Fenton chemistry, where both dissolved Cu ions and nano-CuO surfaces efficiently generate reactive nitrogen species as disinfectants. This simple, efficient, and cost-effective approach for biofilm removal generates important insights into Fenton chemistry, a fundamental mechanism in nature, considering the ubiquity of copper, hydrogen peroxide, and nitrite in the environment, biological systems, and various industrial processes.
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http://dx.doi.org/10.1039/d1cc00374gDOI Listing
May 2021

Comparison between voltammetric detection methods for abalone-flavoring liquid.

Open Life Sci 2021 15;16(1):354-361. Epub 2021 Apr 15.

Mechanical Engineering Department of Dalian Polytechnic University, Dalian 116034, China.

This article attempts to determine the most accurate classification method for different abalone-flavoring liquids. Three common voltammetric detection methods, namely, linear sweep voltammetry (LSV), cyclic voltammetry (CV), and square-wave voltammetry (SWV), were considered. To compare their classification accuracies of abalone-flavoring liquids, three methods were separately adopted to classify five different abalone-flavoring liquids, using a four-electrode (Au, Pt, Pd, and W) sensor array. Then the data acquired by each method were subject to the principal component analysis (PCA): the first three principal components whose eigenvalues were greater than 1 were extracted from each set of data; the cumulative variance contribution rate and the principal component scores of each method were obtained. The PCA results show that the first three principal components obtained by the CV had the highest cumulative variance contribution rate (91.307%), indicating that the CV can more comprehensively characterize the information of abalone-flavoring liquid samples than the other two methods. According to the principal component scores, compared with those of LSV and SWV, the same kind of samples detected by the CV were highly clustered and the different kinds of samples detected by the CV were greatly dispersed. This indicates that the CV can effectively distinguish between the five abalone-flavoring liquids. Finally, the detection data were further verified through probabilistic neural network and a support vector machine algorithm optimized by genetic algorithm. The results further confirm that the CV is more accurate than the other two methods in the classification of abalone-flavoring liquids. Therefore, the CV was recommended for the classification of abalone-flavoring liquids.
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http://dx.doi.org/10.1515/biol-2021-0035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051168PMC
April 2021

Axon-enriched lincRNA ALAE is required for axon elongation via regulation of local mRNA translation.

Cell Rep 2021 May;35(5):109053

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China; Shanghai Research Center for Brian Science and Brain-Inspired Intelligence, Shanghai, China. Electronic address:

Long intergenic noncoding RNAs (lincRNAs) are critical regulators involved in diverse biological processes. However, the roles and related mechanisms of lincRNAs in axon development are largely unknown. Here we report an axon-enriched lincRNA regulating axon elongation, referred to as ALAE. Profiling of highly expressed lincRNAs detected abundant and enriched ALAE in the axons of dorsal root ganglion (DRG) neurons, where it locally promoted axon elongation. Mechanically, ALAE directly interacted with the KH3-4 domains of KH-type splicing regulatory protein (KHSRP) and impeded its association with growth-associated protein 43 (Gap43) mRNA. Knockdown of ALAE reduced the protein but not the mRNA level of GAP43 in the axons of DRG neurons. Furthermore, local disruption of the interaction between ALAE and KHSRP in the axon significantly inhibited Gap43 mRNA translation, impairing axon elongation. This study implies crucial roles of axon-enriched lincRNAs in spatiotemporal regulation of local translation during axon development.
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http://dx.doi.org/10.1016/j.celrep.2021.109053DOI Listing
May 2021

Sensory-based approaches in psychiatric care: A systematic mixed-methods review.

J Adv Nurs 2021 May 5. Epub 2021 May 5.

School of Nursing, Jilin University, Jilin, People's Republic of China.

Aims: Sensory-based approaches, including sensory room, sensory cart and specific sensory integration programs, feature various sensory stimulations to focus on a particular space or program. This systematic mixed-methods review describes the impact of sensory-based approaches in psychiatric care and summarizes the important components of sensory interventions.

Design: Systematic mixed-methods review was based on the guidelines by Pluye and Hong for comprehensively searching, appraising and synthesizing research evidence.

Data Sources: Data were collected from five databases: CINAHL, Embase, Pubmed, Web of Science and Cochrane before March 9, 2020.

Review Methods: Qualitative, quantitative, mixed-methods and original studies published in English on sensory-based approaches in psychiatric care were included. The studies were selected by screening titles, abstracts and full texts, and the quality of each study was assessed by two researchers independently. The data were analysed using thematic analysis.

Results: Sixteen studies were chosen for review. Through data integration, four subthemes with positive effects were formed: (1) calming of the patient's mood; (2) calming of the patient's body; (3) improvement of self-care ability; and (4) improvement of the nurse-patient relationship. The sensory-based approach may also lead to negative effects.

Conclusions: Several important components play important roles in the sensory-based approaches: (1) rich, culture-based, personalized sensory stimulation; (2) a quiet, safe, home-based physical environment; (3) a good one-to-one nurse-patient relationship; (4) and the cultivation of patient autonomy and self-management. Sensory-based approaches in a multicultural environment and home environment will be important topics of psychiatric care in the future.

Impact: There is a lack of synthesis of studies on results of sensory-based approaches in psychiatric care. Four components are important to sensory interventions. It is necessary for mental health service centres and home care for the patients with psychotic disorders to use sensory-based approaches for reference.
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http://dx.doi.org/10.1111/jan.14884DOI Listing
May 2021

Toward an Understanding of the Structural and Mechanistic Aspects of Protein-Protein Interactions in 2-Oxoacid Dehydrogenase Complexes.

Life (Basel) 2021 Apr 29;11(5). Epub 2021 Apr 29.

Department of Chemistry, Rutgers, The State University of New Jersey, Newark, NJ 07102, USA.

The 2-oxoglutarate dehydrogenase complex (OGDHc) is a key enzyme in the tricarboxylic acid (TCA) cycle and represents one of the major regulators of mitochondrial metabolism through NADH and reactive oxygen species levels. The OGDHc impacts cell metabolic and cell signaling pathways through the coupling of 2-oxoglutarate metabolism to gene transcription related to tumor cell proliferation and aging. is a gene encoding 2-oxoadipate dehydrogenase (E1a), which functions in the L-lysine degradation pathway. The potentially damaging variants in have been associated to the (neuro) pathogenesis of several diseases. Evidence was obtained for the formation of a hybrid complex between the OGDHc and E1a, suggesting a potential cross talk between the two metabolic pathways and raising fundamental questions about their assembly. Here we reviewed the recent findings and advances in understanding of protein-protein interactions in OGDHc and 2-oxoadipate dehydrogenase complex (OADHc), an understanding that will create a scaffold to help design approaches to mitigate the effects of diseases associated with dysfunction of the TCA cycle or lysine degradation. A combination of biochemical, biophysical and structural approaches such as chemical cross-linking MS and cryo-EM appears particularly promising to provide vital information for the assembly of 2-oxoacid dehydrogenase complexes, their function and regulation.
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http://dx.doi.org/10.3390/life11050407DOI Listing
April 2021

Thromboelastography-Based Profiling of Coagulation Status in Patients Undergoing Bariatric Surgery: Analysis of 422 Patients.

Obes Surg 2021 Apr 30. Epub 2021 Apr 30.

Department of Anesthesiology, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX, USA.

Introduction/purpose: Some clinical indicators suggest hypercoagulability/hyperaggregability in patients with morbid obesity. Thromboelastography (TEG®) has been used to profile coagulation status in surgical patients. We aimed to assess coagulation profiles in patients with morbid obesity undergoing bariatric surgery by correlating demographic and patient characteristics to pre-operative TEG® values.

Materials And Methods: Pre-operative TEG® values from 422 patients undergoing bariatric surgery were evaluated. TEG® results were analyzed by gender, use of medications known to alter the coagulation profile, and body mass index (BMI).

Results: Patients have a mean of 45.03 ± 11.8 years, female (76.3%), and with a mean BMI of 42 kg/m . The overall coagulation profile of female patients was significantly different from males, even in the sub-cohort without use of medications known to alter coagulation. The majority of patients (94%) with a G value > 15 dynes/cm (clot strength) were female. In females, there was no association between BMI and TEG® values; however, in men, there was a statistically significant difference in TEG® values for those with BMI < 40 kg/m compared to those with BMI > 50 kg/m.

Conclusions: TEG®-based analysis of coagulation profiles offers unique insights. Compared to laboratory normal values (R time, angle, maximal amplitude, and G values), patients with morbid obesity may have a tendency for hypercoagulability/hyperaggregability, with mean values at the higher limit. A significant hypercoagulable difference in TEG® values was identified in female as compared to male patients. Male patients with a BMI greater than 50 kg/m were also found to be increasingly hypercoagulable.
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http://dx.doi.org/10.1007/s11695-021-05445-3DOI Listing
April 2021

SLCO4A1-AS1 regulates laryngeal squamous cell carcinoma cell phenotypes via the Wnt pathway.

Oral Dis 2021 Apr 29. Epub 2021 Apr 29.

Department of Otolaryngology, Yongchuan Hospital (T.C.M) of Chongqing Medical University, Chongqing, China.

Aim: Long non-coding RNAs were widely reported to regulate laryngeal squamous cell carcinoma (LSCC), a prevalent tumor in the head and neck. We aimed to investigate the role of solute carrier organic anion transporter family member 4A1 antisense RNA 1 (SLCO4A1-AS1) in LSCC.

Materials & Methods: CCK-8 and colony formation assays were conducted to examine the viability and proliferation of LSCC cells. The apoptosis of LSCC cells was evaluated using flow cytometry and TUNEL assays. The distribution of SLCO4A1-AS1 in LSCC cells was detected by subcellular fractionation assay. The interaction between molecules was confirmed using luciferase reporter assay.

Results: SLCO4A1-AS1 was overexpressed in LSCC tissues and cells. Furthermore, silenced SLCO4A1-AS1 repressed the proliferation and facilitated apoptosis of LSCC cells. Mechanistical investigation revealed that SLCO4A1-AS1 was a competing endogenous RNA (ceRNA) to upregulate SETD7 by binding with miR-7855-p. Additionally, SLCO4A1-AS1 positively regulated the Wnt/β-catenin signaling pathway by upregulating SETD7. Rescue experiments demonstrated that SLCO4A1-AS1 promoted LSCC proliferation and inhibited LSCC apoptosis by upregulation of SETD7 and activation of the Wnt/β-catenin pathway.

Conclusion: SLCO4A1-AS1 promotes proliferation and inhibits apoptosis of LSCC cells by upregulation of SETD7 in a miR-7855-5p dependent way to activate the Wnt/β-catenin pathway.
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http://dx.doi.org/10.1111/odi.13893DOI Listing
April 2021

ASO Author Reflection: Use of Endobronchial Ultrasound in the Evaluation of Recurrent Laryngeal Nerve Lymph Nodes in Esophageal Squamous Cell Carcinoma Patients.

Ann Surg Oncol 2021 Apr 28. Epub 2021 Apr 28.

Department of Thoracic Oncology, Sun Yat-sen University Cancer Centre, Guangzhou, China.

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http://dx.doi.org/10.1245/s10434-020-09298-5DOI Listing
April 2021

Fine Needle Biopsy Versus Core Needle Biopsy Combined With/Without Thyroglobulin or BRAF 600E Mutation Assessment for Detecting Cervical Nodal Metastasis of Papillary Thyroid Carcinoma.

Front Endocrinol (Lausanne) 2021 12;12:663720. Epub 2021 Apr 12.

Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, China.

Objectives: To analyze the diagnostic benefit of fine needle aspiration biopsy cytology (FNAB-C) and core needle biopsy tissue (CNB-T) with the addition of thyroglobulin (Tg) in the washout of the needle or BRAF V600E mutation assessment in assessing cervical lymph node metastasis (LNM) in papillary thyroid carcinoma.

Materials And Methods: A total of 186 lymph nodes were punctured by fine or core needle. The diagnostic performance of FNAB-C and CNB-T with Tg in the washout or BRAF V600E mutation assessment was compared.

Results: The optimal cutoff value of FNAB-Tg was 1.0 ng/ml, with an AUC of 0.976. The sensitivity and specificity of FNAB-C in predicting cervical LNM were 97.4% and 71.4%, respectively, and the addition of FNAB-Tg could contribute to a sensitivity of 100% and a specificity of 95%, but the introduction of BRAF V600E mutation assessment was associated with a decreased sensitivity of 96.3% and a decreased specificity of 50.0%. The FNAB-Tg level showed a comparable distribution in malignant lymph nodes with different TgAb statuses, serum TSH levels, and serum Tg levels. The sensitivity and specificity of CNB-T in predicting cervical LNM were 98.9% and 100%, respectively. The addition of CNB-Tg did not alter the diagnostic ability, but the introduction of BRAF V600E mutation assessment obtained the best performance, with a sensitivity of 100% and specificity of 100%.

Conclusion: The sensitivity and specificity of FNAB-C could be increased if combined with FNAB-Tg. CNB-T alone could provide satisfactory diagnostic reliability.
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http://dx.doi.org/10.3389/fendo.2021.663720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072388PMC
April 2021

Correction to: lipase-catalyzed transesterification of soybean oil and phytosterol in supercritical CO.

Bioprocess Biosyst Eng 2021 Apr 27. Epub 2021 Apr 27.

Collaborative Innovation Food Safety and Nutrition Center, Wuxi, 214122, China.

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http://dx.doi.org/10.1007/s00449-021-02575-xDOI Listing
April 2021

fucoidan modifies gut microbiota and intestinal metabolites during alleviation of hyperglycemia in type 2 diabetic mice.

Food Funct 2021 Apr;12(8):3572-3585

College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.

Type 2 diabetic mellitus (T2DM) is a complicated metabolic disorder that is now considered as a major global public health problem. Fucoidan exhibits diverse biological activities, especially prevention of metabolic diseases. In this regard, we herein aimed to reveal the beneficial effect of Sargassum fusiforme fucoidan (SFF) on high-fat diet (HFD) and streptozotocin (STZ) induced T2DM mice. We noted that on the one hand, SFF significantly decreased fasting blood glucose, diet and water intake, and hyperlipidemia, while on the other hand, it improved glucose tolerance. Furthermore, SFF reduced epididymal fat deposition, attenuated the pathological changes in heart and liver tissues, and decreased oxidative stress in diabetic mice. To explore the underlying mechanisms of these ameliorative effects, the gut microbiota was analyzed. Notably, SFF highly enriched benign microbes including Bacteroides, Faecalibacterium and Blautia, as well as increased levels of (R)-carnitine and choline in the colon of diabetic mice. This may be a potential mechanism for alleviating T2DM, thus implying the benefits of SFF as an adjuvant agent for T2DM treatment. Taken together, this study demonstrated a promising application of fucoidan as one of the adjuvant agents for the management of T2DM in the future.
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http://dx.doi.org/10.1039/d0fo03329dDOI Listing
April 2021

Status of Immunotherapy Acceptance in Chinese Patients With Multiple Sclerosis: Analysis of .

Front Neurol 2021 8;12:651511. Epub 2021 Apr 8.

Department of Neurology, Affiliated Zhongshan Hospital, Xiamen University, Xiamen, China.

The prevalence of multiple sclerosis (MS) in China is low, although it has been increasing recently. Owing to the paucity of data on immunotherapy acceptance in the Chinese population, we conducted this study to analyze factors affecting the acceptance of immunotherapy and selection of disease-modifying therapies (DMTs) based on personal and clinical data of patients with MS. In this study, data were obtained from the , which was the first national survey of patients with MS in China. There were 1,212 patients with MS from 31 provinces who were treated at 49 Chinese hospitals over a 4-month period from May 2018 to August 2018, and the patients were asked to complete online questionnaires to assess their understanding of the disease. In general, highly educated patients with frequent relapses were more willing to receive treatment regardless of DMTs or other immunotherapy, and patients with more understanding of the disease opted to be treated. Younger patient population, patients with severe disease course, and those with more symptoms were likely to choose the treatment. Moreover, a higher proportion of women chose to be treated with DMTs than with other immunotherapies. Education status and patient awareness of the disease impact the treatment acceptance in Chinese patients with MS. Therefore, we call for improving the awareness of MS disease and social security to help patients to improve their quality of life.
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http://dx.doi.org/10.3389/fneur.2021.651511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060470PMC
April 2021

Comparison of properties of dust in alveolar of rats and the workplace.

Exp Lung Res 2021 May-Jun;47(5):239-249. Epub 2021 Apr 26.

Hebei Province Key Laboratory of Occupational Health and Safety for Coal Industry, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, P.R. China.

Objectives: The purpose of this study was to analyze the α-SiO content, composition, dispersion, morphology, and free radical content of dust between the alveolar and the workplace, to explore the possible changes in the properties (especially the pathogenicity) of dust after it enters the lung.

Methods: We collected the dust in the workplace in HANDAN Coal mine. They were selected by a 400 mesh sieve and was made a suspension of 50 mg/ml, which would be used to perfuse into the trachea of rats. When one week, four weeks, eight weeks, fourteen weeks, twenty weeks after perfusing, we harvested dust in rats alveolar through lung lavage for further processing.

Results: In the animal test, typical fibrous nodules appeared 20 weeks after dust exposure. No inflammatory reaction was observed in the saline group. The results of animal experiments showed that there was no significant difference in the content of α-SiO between dust in the workplace and the lung lavage ( > 0.05). The content of the Fe element gradually increased with dust exposure time. The 12 elements of Al, Mg, Si, Pb, Mn, Ni, Zn, Cu, Cr, Sb, Cd, and AS were reduced in the experiment group compared with the workplace group. The shape of the dust in the workplace was mostly spherical. The shape of the dust extracted from the lung lavage fluid was mostly blocky and angular, and a few dust edges were sharp, and more than 80% of the particle size was smaller than 5 μm, while less than 1% of the particle size was larger than 10 μm. The amount of hydroxyl radical released by lung lavage dust in phosphate buffer was higher than that of the workplace dust.

Conclusions: After the dust entered the alveoli, the content of α-SiO in the dust did not change with dust exposure time, while the content of elements in the dust, the morphology, and dispersion of the dust changed. The ability of dust in alveoli to produce hydroxyl radicals in phosphate buffer was higher than that in the workplace.
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http://dx.doi.org/10.1080/01902148.2021.1916649DOI Listing
April 2021

Chondroblastoma of the patella with secondary aneurysmal bone cyst, an easily misdiagnosed bone tumor:a case report with literature review.

BMC Musculoskelet Disord 2021 Apr 23;22(1):381. Epub 2021 Apr 23.

Ningxia Medical University, 1160 Shengli Street, Xingqing District, Yinchuan, 750004, People's Republic of China.

Background: Chondroblastoma (CB) is a rare, primary, benign bone tumor that commonly affects men aged 15-20 years. It is usually detected in the epiphysis of the long bones, such as the proximal femur, humerus, and tibia. The patella is an infrequent site. CB with secondary aneurysmal bone cyst (ABC) is extremely rare in the patella, which can be easily confused with other common bone tumors of the patella. Thus, it is necessary to make the right diagnosis to get a good outcome.

Case Presentation: We have presented here the case of a 30-year-old man who was suffering from anterior knee pain for the past 6 months that had aggravated 2 weeks before the presentation. Osteolytic bone destruction in the patella could be detected in both his X-ray and computed tomography (CT) examinations, while the magnetic resonance imaging (MRI) detected a fluid level. Accordingly, secondary ABC was presumed. We diagnosed the condition as giant cell tumor (GCT) with secondary ABC and, accordingly, performed curettage inside the focus region with autogenous bone grafting following the patient's medical history, physical manifestations, results of physical and ancillary examinations, and the disease characteristics. However, the intraoperative and postoperative outcomes indicated that the patient's histopathology was consistent with that of typical CB, suggesting a definitive error in diagnosis. Accordingly, the patient was finally diagnosed with patella CB along with secondary ABC.

Conclusions: Past studies have demonstrated that the 3 commonest bone tumors affecting the patella are GCT, CB, and ABC. CB with secondary ABC can be easily misdiagnosed as GCT with secondary ABC or ABC. Performing incision biopsy or excision biopsy and conducting histological examination may be the most effective method for suspected CB with secondary ABC.
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http://dx.doi.org/10.1186/s12891-021-04262-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066474PMC
April 2021

PMN-derived netrin-1 attenuates cardiac ischemia-reperfusion injury via myeloid ADORA2B signaling.

J Exp Med 2021 Jun;218(6)

Department of Anesthesiology, The University of Texas Health Science Center at Houston, McGovern Medical School, Houston, TX.

Previous studies implicated the neuronal guidance molecule netrin-1 in attenuating myocardial ischemia-reperfusion injury. However, the tissue-specific sources and receptor signaling events remain elusive. Neutrophils are among the first cells responding to an ischemic insult and can be associated with tissue injury or rescue. We found netrin-1 levels were elevated in the blood of patients with myocardial infarction, as well as in mice exposed to myocardial ischemia-reperfusion. Selectively increased infarct sizes and troponin levels were found in Ntn1loxP/loxP Lyz2 Cre+ mice, but not in mice with conditional netrin-1 deletion in other tissue compartments. In vivo studies using neutrophil depletion identified neutrophils as the main source for elevated blood netrin-1 during myocardial injury. Finally, pharmacologic studies using treatment with recombinant netrin-1 revealed a functional role for purinergic signaling events through the myeloid adenosine A2b receptor in mediating netrin-1-elicited cardioprotection. These findings suggest an autocrine signaling loop with a functional role for neutrophil-derived netrin-1 in attenuating myocardial ischemia-reperfusion injury through myeloid adenosine A2b signaling.
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http://dx.doi.org/10.1084/jem.20210008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077173PMC
June 2021

Highly Stable Waterborne Luminescent Inks Based on MAPbBr@PbBr(OH) Nanocrystals for LEDs and Anticounterfeit Applications.

ACS Appl Mater Interfaces 2021 May 22;13(17):20622-20632. Epub 2021 Apr 22.

Key Laboratory of Polar Materials and Devices, Ministry of Education, Department of Electronics, East China Normal University, Dongchuan Road 500, Shanghai 200241, P. R. China.

Waterborne polymers are advantageous in terms of cost, convenience, sustainability, and environmental friendliness. As lead halide perovskite (LHP) nanocrystals suffer from fast degradation in the presence of water, it is challenging to encapsulate LHP nanocrystals in waterborne polymers. In this work, luminescent MAPbBr@PbBr(OH) nanocrystals were synthesized via the aqueous grinding process in the presence of 2-methyl-imidazole (2-MIM) and oleylamime (OAm). 2-MIM triggers the formation of the PbBr(OH) matrix, and OAm acts as a size-control ligand to control the size of MAPbBr@PbBrOH particles in the nanoscale range. Highly stable waterborne luminescent inks were successfully prepared by blending MAPbBr@PbBr(OH) nanocrystals with waterborne polymers, including poly(vinylpyrrolidone), poly(vinyl acetate), and acrylate resins. Owning to the dual protection of the polymer matrix and PbBr(OH) to LHP quantum dots (QDs), the luminescent films exhibit excellent stability to the environment under thermal and light irradiation. The ink can be used as a phosphor to fabricate down-converting green and white light-emitting diodes (LEDs). Waterborne anticounterfeiting inks suitable for screen printing were prepared via formula tuning for the anticounterfeit purpose. The anticounterfeiting luminescent patterns can be screen printed on paper, cloth, and poly(ethylene terephthalate) (PET), with encryption and decryption of information being accurately and conveniently realized by switching UV irradiation.
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http://dx.doi.org/10.1021/acsami.1c01905DOI Listing
May 2021

Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota.

Anal Chem 2021 05 22;93(17):6594-6598. Epub 2021 Apr 22.

Ottawa Institute of Systems Biology and Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada.

Lysine acylations are important post-translational modifications that are present in both eukaryotes and prokaryotes and regulate diverse cellular functions. Our knowledge of the microbiome lysine acylation remains limited due to the lack of efficient analytical and bioinformatics methods for complex microbial communities. Here, we show that the serial enrichment using motif antibodies successfully captures peptides containing lysine acetylation, propionylation, and succinylation from human gut microbiome samples. A new bioinformatic workflow consisting of an unrestricted database search confidently identified >60,000 acetylated, and ∼20,000 propionylated and succinylated gut microbial peptides. The characterization of these identified modification-specific metaproteomes, i.e., meta-PTMomes, demonstrates that lysine acylations are differentially distributed in microbial species with different metabolic capabilities. This study provides an analytical framework for the study of lysine acylations in the microbiome, which enables functional microbiome studies at the post-translational level.
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http://dx.doi.org/10.1021/acs.analchem.1c00962DOI Listing
May 2021

Diagnostic KASP Markers of Wheat Broad-spectrum Powdery Mildew Resistance Genes Pm21, PmV and Pm12 Developed for High Throughput Marker-assisted Selection.

Plant Dis 2021 Apr 21. Epub 2021 Apr 21.

Yantai University, No.30, Qingquan Road, Yantai, Shandong, China, 264005;

Wheat powdery mildew is a devastating disease that seriously threatens yield worldwide. Utilization of host resistance is considered an effective strategy to minimize powdery mildew damage. Pm21, PmV, and Pm12 confer broad-spectrum resistance to wheat powdery mildew in China, of which Pm21 and PmV are allelic genes derived from the 6VS chromosome of Dasypyrum villosum, and Pm12 is derived from the 6SS chromosome of Aegilops speltoides and most likely orthologous to the former two genes. To accurately and efficiently transfer and pyramid these genes using marker-assisted selection (MAS), distinctive single nucleotide polymorphisms (SNPs) among the exon sequences of Pm21, PmV, and Pm12 and their homologous sequences in the common wheat genome were identified and used for developing diagnostic Kompetitive Allele-Specific PCR (KASP) markers. The markers were validated in different genotypes including transgenic vectors, transgenic lines, translocation lines, resistance stocks with documented Pm genes, and in multiple susceptible cultivars without Pm genes. As a result, we initially developed a KASP marker that can simultaneously diagnose Pm21, Pm12, and PmV. Subsequently, we obtained a highly diagnostic KASP marker for each of the three genes that could distinguish among the three genes and also accurately distinguish them from other resistant stocks with documented Pm genes and from multiple susceptible genotypes. Compared with previously reported markers, the highly diagnostic KASP markers developed in this study have the advantages of low cost, easy assay, accuracy, and potentially high throughput for MAS.
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http://dx.doi.org/10.1094/PDIS-02-21-0308-REDOI Listing
April 2021

Anti-Tumor Metastasis via Platelet Inhibitor Combined with Photothermal Therapy under Activatable Fluorescence/Magnetic Resonance Bimodal Imaging Guidance.

ACS Appl Mater Interfaces 2021 May 20;13(17):19679-19694. Epub 2021 Apr 20.

Department of Ultrasound, Peking University Third Hospital, Beijing 100191, P. R. China.

Photothermal therapy (PTT) is a promising tumor therapy strategy; however, heterogeneous heat distribution over the tumor often exists, resulting in insufficient photothermal ablation and potential risk of cancer metastasis, which has been demonstrated to be associate with platelets. Herein, a near-infrared (NIR) photothermal agent of IR780 was conjugated with MRI agent of Gd-DOTA via a disulfide linkage (ICD-Gd), which was coassembly with lipid connecting tumor-homing pentapeptide CREKA (Cys-Arg-Glu-Lys-Ala) (DSPE-PEG-CREKA) to encapsulate a platelet inhibitor of ticagrelor (Tic), affording a multistimuli-responsive nanosystem (DPC@ICD-Gd-Tic). The nanosystem with completely quenching fluorescence could specifically target the tumor-associated platelets and showed pH/reduction/NIR light-responsive drug release, which simultaneously resulting in dis-assembly of nanoparticle and fluorescence recovery, enabling the drug delivery visualization in tumor in situ via activatable NIR fluorescence/MR bimodal imaging. Finally, DPC@ICD-Gd-Tic further integrated the photoinduced hyperthermia and platelet function inhibitor to achieve synergistic anticancer therapy, leading to ablation of primary tumor cells and effectively suppressed their distant metastasis. The number of lung metastases in 4T1 tumor bearing mice was reduced by about 90%, and the size of tumor was reduced by about 70%, while half of the mouse was completely cured by this smart nanosystem.
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http://dx.doi.org/10.1021/acsami.1c02302DOI Listing
May 2021