Publications by authors named "Xu Tian"

511 Publications

Network Pharmacology-Based Analysis on the Potential Biological Mechanisms of Sinisan Against Non-Alcoholic Fatty Liver Disease.

Front Pharmacol 2021 27;12:693701. Epub 2021 Aug 27.

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.

Non-alcoholic fatty liver disease (NAFLD) has become the most prevalent liver disease in China. Sinisan (SNS) is a traditional Chinese medicine formula that has been widely used in treating chronic liver diseases, including NAFLD. However, its underlying biological mechanisms are still unclear. In this study, we employed a network pharmacology approach consisting of overlapped terms- (genes or pathway terms-) based analysis, protein-protein interaction (PPI) network-based analysis, and PPI clusters identification. Unlike the previous network pharmacology study, we used the shortest path length-based network proximity algorithm to evaluate the efficacy of SNS against NAFLD. And we also used random walk with restart (RWR) algorithm and Community Cluster (Glay) algorithm to identify important targets and clusters. The screening results showed that the mean shortest path length between genes of SNS and NAFLD was significantly smaller than degree-matched random ones. Six PPI clusters were identified and ten hub targets were obtained, including STAT3, CTNNB1, MAPK1, MAPK3, AGT, NQO1, TOP2A, FDFT1, ALDH4A1, and KCNH2. The experimental study indicated that SNS reduced hyperlipidemia, liver steatosis, and inflammation. Most importantly, JAK2/STAT3 signal was inhibited by SNS treatment and was recognized as the most important signal considering the network pharmacology part. This study provides a systems perspective to study the relationship between Chinese medicines and diseases and helps to discover potential mechanisms by which SNS ameliorates NAFLD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2021.693701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430321PMC
August 2021

A trail pheromone mediates the mutualism between ants and aphids.

Curr Biol 2021 Sep 2. Epub 2021 Sep 2.

College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding 071002, P.R. China; State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Laboratory of Fundamental and Applied Research in Chemical Ecology (FARCE), University of Neuchâtel, 2000 Neuchâtel, Switzerland. Electronic address:

Mutualisms, such as the ones between ants and aphids, evolve and persist when benefits outweigh the costs from the interactions between the partners. We show here that the trail pheromone of the red imported fire ant, Solenopsis invicta, can enhance these benefits by suppressing aphid dispersal and stimulating their reproduction. The ant's mutualistic partner, the cotton aphid Aphis gossypii, was found to readily perceive and respond to two specific trail pheromone components. Two pheromone components, Z,E-α-farnesene and E,E-α-farnesene, both suppressed walking dispersal of apterous aphids, whereas only the major pheromone component, Z,E-α-farnesene, also increased aphid reproduction rate. The ants, as well as the aphids, benefit from this inter-species function of the trail pheromone. For the ants it increases and prolongs the availability of honeydew as a key food source, whereas the aphid colony benefits from faster population growth and continuous ant-provided protection. These findings reveal a hitherto unknown mechanism by which ants and aphids both increase the benefits that they provide to each other, thereby likely enhancing the stability of their mutualistic relationship.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cub.2021.08.032DOI Listing
September 2021

Ikzf2 Regulates the Development of ICOS Th Cells to Mediate Immune Response in the Spleen of -Infected C57BL/6 Mice.

Front Immunol 2021 12;12:687919. Epub 2021 Aug 12.

State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Background: Th cells (helper T cells) have multiple functions in () infection. Inducible co-stimulator (ICOS) is induced and expressed in activated T lymphocytes, which enhances the development of B cells and antibody production through the ICOS/ICOSL pathway. It remains unclear about the role and possible regulating mechanism of ICOS Th cells in the spleen of -infected C57BL/6 mice.

Methods: C57BL/6 mice were infected with cercariae of through the abdomen. The expression of ICOS, activation markers, and the cytokine production on CD4 ICOS Th cells were detected by flow cytometry (FCM) and quantitative real-time PCR (qRT-PCR). Moreover, the differentially expressed gene data of ICOS and ICOS Th cells from the spleen of infected mice were obtained by mRNA sequencing. Besides, Western blot and chromatin immunoprecipitation (ChIP) were used to explore the role of Ikzf2 on ICOS expression.

Results: After infection, the expression of ICOS molecules gradually increased in splenic lymphocytes, especially in Th cells ( < 0.01). Compared with ICOS Th cells, more ICOS Th cells expressed CD69, CD25, CXCR5, and CD40L ( < 0.05), while less of them expressed CD62L ( < 0.05). Also, ICOS Th cells expressed more cytokines, such as IFN-γ, IL-4, IL-10, IL-2, and IL-21 ( < 0.05). RNA sequencing results showed that many transcription factors were increased significantly in ICOS Th cells, especially Ikzf2 ( < 0.05). And then, the expression of Ikzf2 was verified to be significantly increased and mainly located in the nuclear of ICOS Th cells. Finally, ChIP experiments and dual-luciferase reporter assay confirmed that Ikzf2 could directly bind to the ICOS promoter in Th cells.

Conclusion: In this study, ICOS Th cells were found to play an important role in infection to induce immune response in the spleen of C57BL/6 mice. Additionally, Ikzf2 was found to be one important transcription factor that could regulate the expression of ICOS in the spleen of -infected C57BL/6 mice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.687919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406689PMC
August 2021

Cordycepin inhibits cell senescence by ameliorating lysosomal dysfunction and inducing autophagy through the AMPK and mTOR-p70S6K pathway.

FEBS Open Bio 2021 Aug 27. Epub 2021 Aug 27.

School of Basic Medical Science, Chengdu Medical College, China.

Cell senescence is closely related to autophagy. In this article, we identified a natural nucleoside analogue, cordycepin, that has the ability to significantly improve lysosomal function, enhance the activity of the lysosomal representative protease cathepsin B (CTSB), and promote the expression of the functional protein lysosomal-associated membrane protein 2 (LAMP2) on the lysosomal membrane. Cordycepin then restores the damaged autophagy level of aging cells by activating the classic AMPK and mTOR-p70S6K signaling pathways, thus inhibiting cell senescence in an H O -induced stress-induced premature senescence (SIPS) cell model. This study provides new theoretical support for the further development of cordycepin and clinical antiaging drugs to inhibit cell senescence and suggests that the regulatory mechanisms of lysosomes in senescent cells should be considered when treating age-related diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/2211-5463.13263DOI Listing
August 2021

Validation and comparison of prognostic scales in Chinese patients with ischemic stroke: a prospective study from CATIS.

Neurol Res 2021 Aug 25:1-8. Epub 2021 Aug 25.

Department of Epidemiology, Tulane University School of Public Health and Tropical Medicine, New Orleans, LA, USA.

Background: : Various tools are currently available to quantify the risks of adverse clinical outcomes after an ischemic stroke. This study aimed to validate and compare prognostic scales among Chinese patients with ischemic stroke.

Methods: : We compared three stroke prognostic scales (Stroke Prognostication using Age and the National Institutes of Health Stroke Scale-100 [SPAN-100], Totaled Health Risks in Vascular Events [THRIVE], and Acute Stroke Registry and Analysis of Lausanne [ASTRAL]) in 3870 Chinese patients with ischemic stroke from the China Antihypertensive Trial in Acute Ischemic Stroke (CATIS). The 2-year primary outcome was a combination of death and major disability (modified Rankin Scale score ≥3).

Results: : Among all the scales, the ASTRAL score had the best accuracy for predicting 2-year prognosis in Chinese patients with ischemic stroke. The C-statistic of the ASTRAL score for the 2-year primary outcome was 0.79 (95% confidence interval [CI]: 0.78-0.80), and the Hosmer-Lemeshow goodness-of-fit test showed that the ASTRAL score fitted Chinese patients with ischemic stroke well (χ = 9.83, = 0.277). The incidences of the primary outcome in the <5%, 5%-9.9%, 10%-19.9%, and ≥20% risk groups based on the ASTRAL scores were 3.93%, 7.55%, 14.29%, and 41.81%, respectively (odds ratio: 1.23; 95% CI: 1.21-1.26; < 0.001).

Conclusion: : The ASTRAL score had higher efficacy than the SPAN-100 and THRIVE scores in predicting 2-year adverse outcomes among Chinese patients with ischemic stroke, suggesting that it could be a valuable risk assessment tool for the 2-year prognosis of such patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/01616412.2021.1959775DOI Listing
August 2021

Genetic Determinants of Increased Body Mass Index Partially Mediate the Effect of Elevated Birth Weight on the Increased Risk of Atrial Fibrillation.

Front Cardiovasc Med 2021 6;8:701549. Epub 2021 Aug 6.

Department of Cardiology, School of Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China.

Although several observational studies have shown an association between birth weight (BW) and atrial fibrillation (AF), controversy remains. In this study, we aimed to explore the role of elevated BW on the etiology of AF. A two-sample Mendelian randomization (MR) study was designed to infer the causality. The genetic data on the associations of single-nucleotide polymorphisms (SNPs) with BW and AF were separately obtained from two large-scale genome-wide association studies with up to 321,223 and 1,030,836 individuals, respectively. SNPs were identified at a genome-wide significant level ( <5 × 10). The inverse variance-weighted (IVW) method was employed to obtain causal estimates as our primary analysis. Sensitivity analyses with various statistical methods were applied to evaluate the robustness of the results, and multivariable MR analysis was conducted to determine whether this association was mediated by the body mass index (BMI). In total, 144 SNPs were identified as the genetic instrumental variables. MR analysis revealed a causal effect of elevated BW on AF (OR = 1.27, 95% CI = 1.14-1.40, = 5.70 × 10). All the results in sensitivity analyses were consistent with the primary result. The effect of BW on AF was attenuated when adjusted for BMI (OR = 1.16, 95% CI = 1.01-1.33, = 0.04). This study indicated that elevated BW was significantly associated with increased lifelong risk of AF, which may be partially mediated by BMI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2021.701549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377229PMC
August 2021

Cubic AgMnSbTe Semiconductor with a High Thermoelectric Performance.

J Am Chem Soc 2021 Sep 19;143(34):13990-13998. Epub 2021 Aug 19.

Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States.

The reaction of MnTe with AgSbTe in an equimolar ratio (ATMS) provides a new semiconductor, AgMnSbTe. AgMnSbTe crystallizes in an average rock-salt NaCl structure with Ag, Mn, and Sb cations statistically occupying the Na sites. AgMnSbTe is a p-type semiconductor with a narrow optical band gap of ∼0.36 eV. A pair distribution function analysis indicates that local distortions are associated with the location of the Ag atoms in the lattice. Density functional theory calculations suggest a specific electronic band structure with multi-peak valence band maxima prone to energy convergence. In addition, AgTe nanograins precipitate at grain boundaries of AgMnSbTe. The energy offset of the valence band edge between AgMnSbTe and AgTe is ∼0.05 eV, which implies that AgTe precipitates exhibit a negligible effect on the hole transmission. As a result, ATMS exhibits a high power factor of ∼12.2 μW cm K at 823 K, ultralow lattice thermal conductivity of ∼0.34 W m K (823 K), high peak of ∼1.46 at 823 K, and high average of ∼0.87 in the temperature range of 400-823 K.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.1c07522DOI Listing
September 2021

Identification of Young's modulus and equivalent spring constraint boundary conditions of the soft tissue with locally observed displacements for endoscopic liver surgery.

Comput Methods Biomech Biomed Engin 2021 Aug 16:1-16. Epub 2021 Aug 16.

State Key Laboratory of Fluid Power & Mechatronic System, Zhejiang University, Hangzhou, China.

In endoscopic surgery, the surgical navigation system needs to calculate the deformation of soft tissue by biomechanical model which requires elastic properties and boundary conditions. However, patient-specific elastic parameters and boundary conditions of soft tissue are hard to measure accurately from the preoperative images, especially the boundary conditions will change during the operation due to the ligament cutting. In addition, simple boundary conditions such as fixed constraints and free-force constraints are not physically adequate to simulate the elastic effect of ligaments attached to the liver. In this paper, we present a novel method to identify the Young's modulus and equivalent spring constraint boundary conditions of a locally observed soft tissue. Based on the spring constraint boundary condition, a two-step inverse algorithm is developed based on the finite element method (FEM) with integration of energy regularized Gauss-Newton (GN) method and -regularized method, which takes external forces and displacements of observable nodes as inputs. A series of numerical simulations and physical hydrogel phantom experiments were conducted. The results of simulation and physical experiments show that the Young's modulus and equivalent spring constraint boundary conditions identified by the proposed method agree well with their setup true values.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10255842.2021.1959556DOI Listing
August 2021

Risk factors of impulsive-compulsive behaviors in PD patients: a meta-analysis.

J Neurol 2021 Aug 9. Epub 2021 Aug 9.

Department of Neurology, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, No. 1 Shangcheng Avenue, Yiwu, 322000, Zhejiang Province, China.

Objective: To summarize the reliable risk factors of impulsive-compulsive behaviors (ICBs) in Parkinson's disease (PD) patients through a meta-analysis on studies in which PD-ICBs were diagnosed by clinical interview.

Methods: PubMed, Embase, Web of Science, CNKI and Wanfang databases were searched. We selected studies ensuring that diagnosis of ICBs in PD patients depends on semi-structured interviews according to the clinical diagnostic criteria of ICBs. The Newcastle-Ottawa Scale was used to evaluate quality of the included studies. The analyzed factors included demographic information, clinical characteristics of PD and medications.

Results: A total of 856 records were screened and 66 full texts were evaluated, and 13 studies (684 PD patients with ICBs [PD-ICBs] and 3,382 PD patients without ICBs [PD-non-ICBs]) were included. Compared with PD-non-ICBs, PD-ICBs were younger in age (- 3.7 [- 5.53, - 1.87], P < 0.0001), with a greater proportion of males (1.64 [1.21, 2.22], P = 0.001), with a younger age of PD onset (- 5.42 [- 7.87, - 2.97], P < 0.0001) and a longer course of PD (1.30 [0.38, 2.22], P = 0.005). PD-ICBs were also associated with higher HAM-D (1.74 [0.47, 3.01], P = 0.007), more levodopa dosage (1.74 [1.09, 2.77], P = 0.02) and dopamine receptor agonists (DA) use (3.96 [2.74, 5.71), P < 0.00001), and higher average dose (levodopa 117.53 [53.59, 181.46], P = 0.0003; DA 80.03 [46.16, 113.90], P < 0.00001), as well as more amantadine use (2.20 [1.42, 3.40], P = 0.0004). The meta-analysis of most factors showed less heterogeneity, except age, age of onset, PD duration, Hoehn and Yahr stage, MMSE and drug dosage. However, whether rapid eye movement sleep behavior disorder, dyskinesia, genetic polymorphism and other factors are risk factors for PD-ICBs remains unclear.

Conclusion: This meta-analysis suggests that males, young, early disease onset, long disease duration, depression, dose of levodopa, dopamine receptor agonists and amantadine are risk factors of ICBs in PD patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00415-021-10724-1DOI Listing
August 2021

Spatial Isolation-Inspired Ultrafine CoSe for High-Energy Aluminum Batteries with Improved Rate Cyclability.

ACS Nano 2021 Aug 6. Epub 2021 Aug 6.

Department of Materials Science, Fudan University, Shanghai 200433, China.

Transition-metal selenides are attractive cathode materials for rechargeable aluminum batteries (RABs) because of their high specific capacity, superior electrical properties, and low cost. To overcome the associated challenges of low structural stability and poor reaction kinetics, a spatial isolation strategy was applied to develop RAB cathodes comprising ultrafine CoSe particles embedded in nitrogen-doped porous carbon nanosheet (NPCS)/MXene hybrid materials; the two-dimensional NPCS structures were derived from the self-assembly of metal frameworks on MXene surfaces. This synthetic strategy enabled control over the particle size of the active materials, even at high pyrolysis temperature, thereby allowing investigations into the effect of size on the electrochemical behavior. Spectroscopic analysis revealed that the CoSe-NPCS electrode exhibited a high discharge capacity (436 mAh g at 1 A g), excellent rate capability (122 mA h g at 5 A g), and long-term cycling stability (212 mAh g after 500 cycles at 1 A g). Theoretical calculations regarding the Co adsorption affinities at various doping sites elucidated the synergistic effects of N-C/MXene hybrids for boosting the reaction kinetics and Co adsorption behavior in this system. This work offers an effective material engineering approach for designing electrodes with high rate stability for high-energy RABs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.1c04895DOI Listing
August 2021

Cost-Effectiveness Analysis of Durvalumab Plus Chemotherapy in the First-Line Treatment of Extensive-Stage Small Cell Lung Cancer.

J Natl Compr Canc Netw 2021 Aug 4. Epub 2021 Aug 4.

1Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan.

Background: In the CASPIAN trial, durvalumab + chemotherapy demonstrated significant improvements in overall survival compared with chemotherapy alone in patients with extensive-stage small cell lung cancer (SCLC). We aimed to assess the cost-effectiveness of durvalumab in patients with extensive-stage SCLC from the US healthcare system perspective.

Patients And Methods: A comprehensive Markov model was adapted to evaluate cost and effectiveness of durvalumab combination versus platinum/etoposide alone in the first-line therapy of extensive-stage SCLC based on data from the CASPIAN study. The main endpoints included total costs, life years (LYs), quality-adjusted life-years (QALYs), and incremental cost-e-ectiveness ratios (ICERs). Model robustness was assessed with sensitivity analysis, and additional subgroup analyses were also performed.

Results: Durvalumab + chemotherapy therapy resulted in an additional 0.27 LYs and 0.20 QALYs, resulting in an ICER of $464,711.90 per QALY versus the chemotherapy treatment. The cost of durvalumab has the greatest influence on this model. Subgroup analyses showed that the ICER remained higher than $150,000/QALY (the willingness-to-pay threshold in the United States) across all patient subgroups.

Conclusions: Durvalumab in combination with platinum/etoposide is not a cost-effective option in the first-line treatment of patients with extensive-stage SCLC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.6004/jnccn.2020.7796DOI Listing
August 2021

Association of the classification and severity of heart failure with the incidence of contrast-induced acute kidney injury.

Sci Rep 2021 Jul 28;11(1):15348. Epub 2021 Jul 28.

Department of Cardiology, Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, 3 East Qingchun Road, Hangzhou, Zhejiang Province, 310016, People's Republic of China.

Congestive heart failure (HF) is a known risk factor of contrast-induced acute kidney injury (CI-AKI). However, the relationship of the classification and severity of HF with CI-AKI remains under-explored. From January 2009 to April 2019, we recruited patients undergoing elective PCI who had complete pre- and post-operative creatinine data. According to the levels of ejection fraction (EF), HF was classified as HF with reduced EF (HFrEF) [EF < 40%], HF with mid-range EF (HFmrEF) [EF 40-49%] and HF with preserved EF (HFpEF) [EF ≥ 50%]. CI-AKI was defined as an increase of either 25% or 0.5 mg/dL (44.2 μmoI/L) in serum baseline creatinine level within 72 h following the administration of the contrast agent. A total of 3848 patients were included in the study; mean age 67 years old, 33.9% females, 48.1% with HF, and 16.9% with CI-AKI. In multivariate logistic regression analysis, HF was an independent risk factor for CI-AKI (OR 1.316, p value < 0.05). Among patients with HF, decreased levels of EF (OR 0.985, p value < 0.05) and elevated levels of N-terminal pro b-type natriuretic peptide (NT-proBNP) (OR 1.168, p value < 0.05) were risk factors for CI-AKI. These results were consistent in subgroup analysis. Patients with HFrEF were more likely to develop CI-AKI than those with HFmrEF or HFpEF (OR 0.852, p value = 0.031). Additionally, lower levels of EF were risk factors for CI-AKI in the HFrEF and HFmrEF groups, but not in the HFpEF group. NT-proBNP was an independent risk factor for CI-AKI in the HFrEF, HFmrEF and HFpEF groups. Elevated levels of NT-proBNP are independent risk factors for CI-AKI irrespective of the classification of HF. Lower levels of EF were risk factors for CI-AKI in the HFrEF and HFmrEF groups, but not in the HFpEF group.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-94910-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319404PMC
July 2021

Outer Membrane Vesicles Derived from Serotype Typhimurium Can Deliver 2a O-Polysaccharide Antigen To Prevent 2a Infection in Mice.

Appl Environ Microbiol 2021 09 10;87(19):e0096821. Epub 2021 Sep 10.

Department of Medical Microbiology, School of Medicine, Nanchang Universitygrid.260463.5, Nanchang, China.

Shigellosis has become a serious threat to health in many developing countries due to the severe diarrhea it causes. Shigella flexneri 2a is the principal species responsible for this endemic disease. Despite multiple attempts to design a vaccine against shigellosis, no effective vaccine has been developed yet. Lipopolysaccharide (LPS) is both an essential virulence factor and an antigen protective against , due to its outer domain, termed O-polysaccharide antigen. In the present study, S. flexneri 2a O-polysaccharide antigen was innovatively biosynthesized in Salmonella and attached to core-lipid A via the ligase WaaL, with purified outer membrane vesicles (OMVs) utilized as vaccine vectors. Here, we identified the expression of the heterologous O-antigen and have described the isolation, characterization, and immune protection efficiency of the OMV vaccine. Furthermore, the results of animal experiments indicated that immunization of mice with the OMV vaccine induced significant specific anti- LPS antibodies in the serum, with similar trends in IgA levels from vaginal secretions and fluid from bronchopulmonary lavage, both intranasally and intraperitoneally. The OMV vaccine derived from both routes of administration provided significant protection against virulent S. flexneri 2a infection, as judged by a serum bactericidal assay, opsonization assay, and challenge test. This vaccination strategy represents a novel and improved approach to control shigellosis by the combination of Salmonella glycosyl carrier lipid bioconjugation with OMVs. , the cause of shigellosis or bacillary dysentery, is a major public health concern, especially for children in developing countries. An effective vaccine would control the spread of the disease to some extent. However, no licensed vaccine against infection in humans has so far been developed. The O-antigen polysaccharide is effective in stimulating the production of protective antibodies and so could represent a vaccine antigen candidate. In addition, bacterial outer membrane vesicles (OMVs) have been used as antigen delivery platforms due to their nanoscale properties and ease of antigen delivery to trigger an immune response. Therefore, the present study provides a new strategy for vaccine design, combining a glycoconjugated vaccine with OMVs. The design concept of this strategy is the expression of O-antigen via the LPS synthesis pathway in recombinant Salmonella, from which the OMV vaccine is then isolated. Based on these findings, we believe that the novel vaccine design strategy in which polysaccharide antigens are delivered via bacterial OMVs will be effective for the development and clinical application of an effective vaccine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/AEM.00968-21DOI Listing
September 2021

Fulminant type I cryoglobulinemic glomerulonephritis with unique ultrastructural plugs: a case report.

Int J Hematol 2021 Jul 24. Epub 2021 Jul 24.

Department of Nephrology, Institute of Nephrology, Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, 197 Ruijin Er Road, Shanghai, 200025, China.

Introduction:  Type I cryoglobulinemia is a rare disease which affects the skin, central nervous system and kidneys. It is usually associated with lymphoproliferative disorders such as multiple myeloma, lymphoma and monoclonal gammopathy of renal significance. Proteinuria and membranoproliferative glomerulonephritis are the most common renal manifestations; Case presentation: Here we report the case of a female patient in her late 40 s who had proteinuria accompanied by Raynaud's phenomenon, high blood and plasma viscosity, hearing loss, and cardiac and central nervous system involvement. Monoclonal immunoglobulin G-λ protein was detected and serum was positive for cryoglobulin. Renal biopsy revealed massive cryo-plugs with unique ultrastructural appearance in the glomerular and peritubular capillary lumina. Immunofluorescence showed predominant IgG3/λ deposition in cryo-plugs. As reported, the clinical manifestations of this patient resulted from cryoprecipitate and hyperviscosity syndrome; Conclusion: Cryoglobulinemia should be considered as a possible diagnosis in patients with Raynaud's phenomenon, hyperviscosity syndrome and monoclonal immunoglobulin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12185-021-03194-8DOI Listing
July 2021

Nanoparticle-Aided Nanoreactor for Nanoproteomics.

Anal Chem 2021 08 23;93(30):10568-10576. Epub 2021 Jul 23.

Department of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, Michigan 48824 United States.

Large-scale bottom-up proteomics of few even single cells is crucial for a better understanding of the roles played by cell-to-cell heterogeneity in disease and development. Novel proteomic methodologies with extremely high sensitivity are required for few even single-cell proteomics. Sample processing with high recovery and no contaminants is one key step. Here we developed a nanoparticle-aided nanoreactor for nanoproteomics (Nano3) technique for processing low-nanograms of mammalian cell proteins for proteome profiling. The Nano3 technique employed nanoparticles packed in a capillary channel to form a nanoreactor (≤30 nL) for concentrating, cleaning, and digesting proteins originally in a lysis buffer containing sodium dodecyl sulfate (SDS), followed by nanoRPLC-MS/MS analysis. The Nano3 method identified a 40-times higher number of proteins based on MS/MS from 2-ng mouse brain protein samples compared to the SP3 (single-pot solid-phase-enhanced sample preparation) method, which performed the sample processing using the nanoparticles in a 10 μL solution in an Eppendorf tube. The data indicates a drastically higher sample recovery of the Nano3 compared to the SP3 method for processing mass-limited proteome samples. In this pilot study, the Nano3 method was further applied in processing 10-1000 HeLa cells for bottom-up proteomics, producing 441 ± 263 ( = 4) (MS/MS) and 983 ± 292 ( = 4) [match between runs (MBR)+MS/MS] protein identifications from only 10 HeLa cells using a Q-Exactive HF mass spectrometer. The preliminary results render the Nano3 method a useful approach for processing few mammalian cells for proteome profiling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.1c01704DOI Listing
August 2021

SERINC2 increases the risk of bipolar disorder in the Chinese population.

Depress Anxiety 2021 Sep 19;38(9):985-995. Epub 2021 Jul 19.

Guangzhou Huiai Hospital, Affiliated Brain Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.

Background: Although common variants in a large collection of patients are associated with increased risk for bipolar disorder (BD), studies have only been able to predict 25%-45% of risks, suggesting that lots of variants that contribute to the risk for BD haven't been identified. Our study aims to identify novel BD risk genes.

Methods: We performed whole-exome sequencing of 27 individuals from 6 BD multi-affected Chinese families to identify candidate variants. Targeted sequencing of one of the novel risk genes, SERINC2, in additional sporadic 717 BD patients and 312 healthy controls (HC) validated the association. Magnetic resonance imaging (MRI) were performed to evaluate the effect of the variant to brain structures from 213 subjects (4 BD subjects from a multi-affected family, 130 sporadic BD subjects and 79 HC control).

Results: BD pedigrees had an increased burden of uncommon variants in extracellular matrix (ECM) and calcium ion binding. By large-scale sequencing we identified a novel recessive BD risk gene, SERINC2, which plays a role in synthesis of sphingolipid and phosphatidylserine (PS). MRI image results show the homozygous nonsense variant in SERINC2 affects the volume of white matter in cerebellum.

Conclusions: Our study identified SERINC2 as a risk gene of BD in the Chinese population.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/da.23186DOI Listing
September 2021

The use of mindfulness-based stress reduction (MBSR) for lung cancer patients: protocol for a systematic review and meta-analysis.

Ann Palliat Med 2021 Jul 21;10(7):8276-8282. Epub 2021 Jun 21.

Nursing Department, Universitat Rovira i Virgili, Tarragona, Spain.

Background: Mindfulness-based stress reduction (MBSR) intervention has been extensively applied in cancer patients for relieving symptom burden and its effectiveness has also been demonstrated. However, the effectiveness of MBSR on psychological and physical functions in lung cancer patients has not yet been determined. The aim of the present systematic review and meta-analysis seeks to determine the role of MBSR in lung cancer patients.

Methods: A systematic search of PubMed, EMBASE, Cochrane Library, and China National Knowledgement Infrastructure (CNKI) will be carried out from their inception until to December 30, 2020. Studies investigating the comparative effects between MBSR and control groups on psychological and physical outcomes will be documented. Data concerning studies, patient characteristics, and outcomes will be extracted. Methodological quality of each eligible randomized controlled trial (RCT) will be assessed individually by two investigators independently using criteria recommended in the Cochrane Handbook for Systematic Reviews of Interventions 5.1.0. Meanwhile, Newcastle-Ottawa Quality Assessment Scale (NOS) will be used to assess methodological quality of non-randomized studies. All statistical analyses will be performed with RevMan and STATA softwares.

Discussion: The role of MBSR in lung cancer patients has not yet been demonstrated. This systematic review and meta-analysis will further determine the effectiveness of MBSR on psychological and physical outcomes and QoL among lung cancer patients, which will provide golden references for developing psychological interventions in order to improve patient care and designing future studies to bridge the gap between research findings and clinical practice.

Trial Registration: We registered the protocol of this systematic review and meta-analysis in Open Science Framework (OSF) platform with a registration DOI of 10.17605/OSF.IO/MWVBQ (available from: https://osf.io/mwvbq).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/apm-21-194DOI Listing
July 2021

Error Bounds of Imitating Policies and Environments for Reinforcement Learning.

IEEE Trans Pattern Anal Mach Intell 2021 Jul 14;PP. Epub 2021 Jul 14.

In sequential decision-making, imitation learning (IL) trains a policy efficiently by mimicking expert demonstrations. Various imitation methods were proposed and empirically evaluated, meanwhile, their theoretical understandings need further studies, among which the compounding error in long-horizon decisions is a major issue. In this paper, we firstly analyze the value gap between the expert policy and imitated policies by two imitation methods, behavioral cloning (BC) and generative adversarial imitation. The results support that generative adversarial imitation can reduce the compounding error compared to BC. Furthermore, we establish the lower bounds of IL under two settings, suggesting the significance of environment interactions in IL. By considering the environment transition model as a dual agent, IL can also be used to learn the environment model. Therefore, based on the bounds of imitating policies, we further analyze the performance of imitating environments. The results show that environment models can be more effectively imitated by generative adversarial imitation than BC. Particularly, we obtain a policy evaluation error that is linear with the effective planning horizon w.r.t. the model bias, suggesting a novel application of adversarial imitation for model-based reinforcement learning (MBRL). We hope these results could inspire future advances in IL and MBRL.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TPAMI.2021.3096966DOI Listing
July 2021

Effect of statins on post-contrast acute kidney injury: a multicenter retrospective observational study.

Lipids Health Dis 2021 Jul 5;20(1):63. Epub 2021 Jul 5.

Department of Cardiovascular Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, No 3 East of Qinchun Road, Hangzhou, Zhejiang, 310000, People's Republic of China.

Background: Post-contrast acute kidney injury (PC-AKI) is a severe complication of coronary angiography (CAG) and percutaneous coronary intervention (PCI). Currently, the effect of statins on PC-AKI and its mechanism remains unclear.

Methods: This multicenter retrospective observational study included 4386 patients who underwent CAG or PCI from December 2006 to December 2019 in Sir Run Run Shaw Hospital and its medical consortium hospitals. Serum creatinine pre- or post-procedure within 72 h after PCI was recorded. Multivariate logical regression was used to explore whether preoperative use of statins was protective from PC-AKI. The path analysis model was then utilized to look for the mediation factors of statins.

Results: Four thousand three hundred eighty-six patients were enrolled totally. The median age of the study population was 68 years old, 17.9% with PC-AKI, and 83.3% on preoperative statins therapy. The incidence of PC-AKI was significantly lower in group of patients on statins therapy. Multivariate regression indicated that preoperative statins therapy was significantly associated with lower percentage of elevated creatinine (β: -0.118, P < 0.001) and less PC-AKI (OR: 0.575, P < 0.001). In the preoperative statins therapy group, no statistically significant difference was detected between the atorvastatin and rosuvastatin groups (OR: 1.052, P = 0.558). Pathway model analysis indicated a direct protective effect of preoperative statins therapy on PC-AKI (P < 0.001), but not through its lipid-lowering effect (P = 0.277) nor anti-inflammatory effect (P = 0.596). Furthermore, it was found that "low-density lipoprotein cholesterol (LDL-C)→C-reactive protein (CRP)" mediated the relationship between preoperative statins therapy and PC-AKI (P = 0.007). However, this only explained less than 1% of the preoperative protective effects of statins on PC-AKI.

Conclusion: Preoperative statins therapy is an independent protective factor of PC-AKI, regardless of its type. This protective effect is not achieved by lipid-lowering effect or anti-inflammatory effect. These findings underscore the potential use of statins in preventing PC-AKI among those at risk.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12944-021-01489-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258930PMC
July 2021

Therapeutic Effect and Cost-Benefit Analysis of Three Different Nutritional Schemes for Esophageal Cancer Patients in the Early Post-operative Period.

Front Nutr 2021 17;8:651596. Epub 2021 Jun 17.

Department of Nutrition, Xinqiao Hospital of Army Medical University, Chongqing, China.

To retrospectively investigate the comparative efficacy, safety and cost-benefits of three nutritional treatment schemes including short peptide jejunal nutrition (SPJN), whole protein jejunal nutrition (WPJN), and partial parenteral nutrition (PPN) in patients underwent esophagectomy for esophageal cancer in our hospital. This study was carried out in accordance with the conceptual framework of nutritional therapy in fast-track rehabilitation surgery. We retrospectively reviewed 305 patients who were assigned to receive esophagectomy for esophageal cancer. Eligible patients was naturally divided into SPJN group [ = 98 (32.1%)], WPJN group [ = 95 (31.1%)], and PPN group [ = 112 (36.7%)] according to the type of nutritional scheme which was actually prescribed to patients by the attendingphysician in clinical practice. The differences of the serum total protein (TP), albumin (ALB), pre-albumin (PA), hemoglobin (HGB), white blood cells (WBCs), red blood cells (RBCs) and neutrophils were compared among 3 nutritional schemes groups. We also investigated the relationship of the fluid intake, urine output, gastric juice drainage volume and thoracic drainage volume among 3 nutritional groups at 3 days after surgery. Moreover, the differences of cost-benefit indexes, complications, length of hospitalization and hospital expenditure were also compared. The serum TP, ALB, and PA in the SPJN group were all higher than those in the WPJN and PPN groups ( < 0.05). The gastric juice volume of gastrointestinal decompression drainage and fluid volume of thoracic drainage in the SPJN group were all less than that in the WPJN group ( < 0.05). The overall hospital stay and post-operative hospital stay in the SPJN group were all shorter than that in WPJN group ( < 0.05). Moreover, the incidence of post-operative complications including anastomotic leakage, infection, and gastrointestinal reaction was remarkably lower in the SPJN group compared to the WPJN group ( < 0.05). Interesting, hospital expenditure in the PPN group was less than that in the SPJN and the WPJN groups ( < 0.001). Patients may obtain benefits in improving protein level after receiving SPJN scheme at the early stage after esophagectomy. Meanwhile, patients may obtain benefits in improving post-operative complications and hospital stay after receiving SPJN or PPN compared to WPJN protocol. However, the difference between SPJN and PPN requires further study because no difference was detected in terms of clinical outcomes including complications and the length of hospitalization although PPN may achieve a possible decrease of medical expenditure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnut.2021.651596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247476PMC
June 2021

Excellent fluorescence detection of Cuin water system using N-acetyl-L-cysteines modified CdS quantum dots as fluorescence probe.

Nanotechnology 2021 Jul 15;32(40). Epub 2021 Jul 15.

Key Lab of Quantum Information of Yunnan Province, Yunnan University, 650091 Kunming, People's Republic of China.

View of the negative influence of metal ions on natural environment and human health, fast and quantitative detection of metals ions in water systems is significant. Ultra-small grain size CdS quantum dots (QDs) modified with N-acetyl-L-cysteines (NALC) (NALC-CdS QDs) are successfully prepared via a facile hydrothermal route. Based on the changes of fluorescence intensity of NALC-CdS QDs solution after adding metal ions, the fluorescence probe made from the NALC-CdS QDs is developed to detect metal ions in water systems. Among various metal ions, the fluorescence of NALC-CdS QDs effectively quenched by the addition of Cu, the probe shows high sensitivity and selectivity for detecting Cuin other interferential metal ions coexisted system. Importantly, the fluorescence intensity of NALC-CdS QDs changes upon the concentration of Cu, the probe displays an excellent linear relationship between the fluorescence quenching rate and the concentration of Cuin ranging from 1 to 25M. Besides, the detected limitation of the probe towards Cuas low as 0.48M. The measurement of Cuin real water sample is also carried out using the probe. The results indicate that NALC-CdS QDs fluorescence probe may be a promising candidate for quantitative Cudetection in practical application.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ac1016DOI Listing
July 2021

Simultaneous determination of 14 pesticide residues in tea by multi-plug filtration cleanup combined with LC-MS/MS.

J Environ Sci Health B 2021 30;56(8):771-781. Epub 2021 Jun 30.

College of Science, China Agricultural University, Beijing, China.

A combined method of multi-plug filtration cleanup (m-PFC) and liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) was established to simultaneously detect 14 pesticides in tea. The pesticides in water-soaked tea were extracted with acetonitrile. Cleanup of tea extract was performed using an m-PFC column packed with multiple cleanup materials: multi-walled carbon nano-tubes (MWCNTs), primary secondary amine (PSA) and anhydrous magnesium sulfate (MgSO). The cleanup effect of the column was evaluated based on the rates of removal of tea components that interfered with pesticide recovery, henceforth referred to as interference components. Results showed that 14 pesticides had strong linearity in the range of 5-500 μg L ( > 0.99). The quantitative limits were within the range of 3-50 μg kg. The average recoveries of 14 pesticides spiked into three different blank tea samples (green tea, black tea, oolong tea) at three levels of 0.05, 0.50 and 2.00 mg kg were in the range of 62.3-108.8% with relative standard deviations of 0.2-13.6%. The m-PFC method can greatly improve the efficiency of sample pretreatment. Furthermore, this work provides methodological guidance on how to select cleanup materials and allocate their proportions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/03601234.2021.1944962DOI Listing
August 2021

The Belt and Road Initiative and the Sustainable Development Goals.

One Earth 2020 Sep;3(3):263-267

The Belt and Road Initiative is perhaps the largest infrastructure development project in history and has aspirations to contribute to the Sustainable Development Goals. In this Voices, we ask experts from various disciplinary backgrounds for their perspectives on the opportunities and challenges for the Belt and Road Initiative in achieving this vision.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.oneear.2020.08.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500279PMC
September 2020

Predicting the Risk of Psychological Distress among Lung Cancer Patients: Development and Validation of a Predictive Algorithm Based on Sociodemographic and Clinical Factors.

Asia Pac J Oncol Nurs 2021 Jul-Aug;8(4):403-412. Epub 2021 May 31.

Department of Nursing, Rovira I Virgili University, Tarragona, Spain.

Objective: Lung cancer patients reported the highest incidence of psychological distress. It is extremely important to identify which patients at high risk for psychological distress. The study aims to develop and validate a predictive algorithm to identify lung cancer patients at high risk for psychological distress.

Methods: This cross-sectional study identified the risk factors of psychological distress in lung cancer patients. Data on sociodemographic and clinical variables were collected from September 2018 to August 2019. Structural equation model (SEM) was conducted to determine the associations between all factors and psychological distress, and then construct a predictive algorithm. Coincidence rate was also calculated to validate this predictive algorithm.

Results: Total 441 participants sent back validated questionnaires. After performing SEM analysis, educational level ( = 0.151, = 0.004), residence ( = 0.146, = 0.016), metastasis ( = 0.136, = 0.023), pain degree ( = 0.133, = 0.005), family history ( = -0.107, = 0.021), and tumor, node, and metastasis stage ( = -0.236, < 0.001) were independent predictors for psychological distress. The model built with these predictors showed an area under the curve of 0.693. A cutoff of 66 predicted clinically significant psychological distress with a sensitivity, specificity, positive predictive value, and negative predictive value of 65.41%, 66.90%, 28.33%, and 89.67%, respectively. The coincidence rate between predictive algorithm and distress thermometer was 64.63%.

Conclusions: A validated, easy-to-use predictive algorithm was developed in this study, which can be used to identify patients at high risk of psychological distress with moderate accuracy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/apjon.apjon-2114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186387PMC
May 2021

Recent advances (2019-2021) of capillary electrophoresis-mass spectrometry for multilevel proteomics.

Mass Spectrom Rev 2021 Jun 15. Epub 2021 Jun 15.

Department of Chemistry, Michigan State University, East Lansing, Michigan, USA.

Multilevel proteomics aims to delineate proteins at the peptide (bottom-up proteomics), proteoform (top-down proteomics), and protein complex (native proteomics) levels. Capillary electrophoresis-mass spectrometry (CE-MS) can achieve highly efficient separation and highly sensitive detection of complex mixtures of peptides, proteoforms, and even protein complexes because of its substantial technical progress. CE-MS has become a valuable alternative to the routinely used liquid chromatography-mass spectrometry for multilevel proteomics. This review summarizes the most recent (2019-2021) advances of CE-MS for multilevel proteomics regarding technological progress and biological applications. We also provide brief perspectives on CE-MS for multilevel proteomics at the end, highlighting some future directions and potential challenges.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mas.21714DOI Listing
June 2021

Appraising the Causal Association of Plasma Homocysteine Levels With Atrial Fibrillation Risk: A Two-Sample Mendelian Randomization Study.

Front Genet 2021 26;12:619536. Epub 2021 May 26.

Key Laboratory of Biotherapy of Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: Although several observational studies have suggested an association of elevated plasma homocysteine (Hcy) levels with increased risk of atrial fibrillation (AF), it remains unclear whether this association reflects causality. In this study, we aimed to investigate the causal association of plasma Hcy levels with AF risk.

Methods: A two-sample Mendelian randomization (MR) study was designed to investigate the causal association of Hcy with AF. Summary data on association of single nucleotide polymorphisms (SNPs) with Hcy were extracted from the hitherto largest genome-wide association study (GWAS) with up to 44,147 individuals, and statistics data on association of SNPs with AF were obtained from another recently published GWAS with up to 1,030,836 individuals. SNPs were selected at a genome-wide significance threshold ( < 5 × 10). Fixed-effect inverse variance weighting (IVW) method was used to calculate the causal estimate. Other statistical methods and leave-one-out analysis were applied in the follow-up sensitivity analyses. MR-Egger intercept test was conducted to detect the potential directional pleiotropy.

Results: In total, nine SNPs were identified as valid instrumental variables in our two-sample MR analysis. Fixed-effect IVW analysis indicated no evidence of causal association of genetically predicted Hcy with AF. The odds ratio (OR) and 95% confidence interval (CI) of AF per standard deviation (SD) increase in Hcy were 1.077 (0.993, 1.168), = 0.075. Similar results were observed in the sensitivity analyses. MR-Egger intercept test suggested no evidence of potential horizonal pleiotropy.

Conclusions: This two-sample MR analysis found no evidence to support causal association of Hcy with AF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.619536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189424PMC
May 2021

Preparation and characterization of octenyl succinic anhydride modified waxy maize starch hydrolyzate/chitosan complexes with enhanced interfacial properties.

Carbohydr Polym 2021 Sep 20;267:118228. Epub 2021 May 20.

Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Wuxi 214122, Jiangsu Province, PR China; School of Food Science and Technology, Jiangnan University, 1800 LiHu Avenue, Wuxi 214122, Jiangsu Province, PR China; Collaborative Innovation Center for Food Safety and Quality Control, Jiangnan University, 1800 LiHu Avenue, Wuxi 214122, Jiangsu Province, PR China. Electronic address:

The preparation and characterization of colloidal complexes based on octenyl succinic anhydride starch hydrolyzate (OSAS) and chitosan (CS) were conducted. Results showed that OSA-S/CS ratio (r) and pH significantly affected complex turbidities and yields. The highest turbidity and yield were obtained at r = 6:1 when pH was fixed, and at pH 6.5 when r was fixed. All complexes remained liquid-like except that formed at pH 6.5, which exhibited a gel structure due to the strongest complexation. OSA-S/CS complexes had intertwined core-shell microstructure and exhibited electrostatic interactions between COO and NH groups of OSA-S and CS, respectively. The complexes prepared at r = 6:1 and pH 6.0 exhibited the most suitable wettability (θ = 91.97°) and interfacial adsorption dynamics. The compact lamellar network and intact cores of these complexes were also shown. This work provides profound and comprehensive information about the formation and physicochemical properties of OSA-S/CS complexes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2021.118228DOI Listing
September 2021

The association between hypertension and nonalcoholic fatty liver disease (NAFLD): literature evidence and systems biology analysis.

Bioengineered 2021 12;12(1):2187-2202

School of Traditional Chinese Medicine, Capital Medical University, Beijing, China.

Nonalcoholic fatty liver disease (NAFLD) has become a major public health issue as its progression increases risks of multisystem morbidity and mortality. Recent evidence indicates a more complex relationship between hypertension and NAFLD than previously thought. In this study, a comprehensive literature search was used to gather information supporting the comorbidity phenomenon of hypertension and NAFLD. Then, systems biology approach was applied to identify the potential genes and mechanisms simultaneously associated with hypertension and NAFLD. With the help of protein-protein interaction network-based algorithm, we found that the distance between hypertension and NAFLD was much less than random ones. Sixty-four shared genes of hypertension and NAFLD modules were identified as core genes. Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis indicated that some inflammatory, metabolic and endocrine signals were related to the potential biological functions of core genes. More importantly, drugs used to treat cardiovascular diseases, hypertension, hyperlipidemia, inflammatory diseases and depression could be potential therapeutics against hypertension-NAFLD co-occurrence. After analyzing public OMICs data, ALDH1A1 was identified as a potential therapeutic target, without being affected by reverse causality. These findings give a clue for the potential mechanisms of comorbidity of hypertension and NAFLD and highlight the multiple target-therapeutic strategy of NAFLD for future clinical research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2021.1933302DOI Listing
December 2021

Cut-off values of lesion and vessel quantitative flow ratio in de novo coronary lesion post-drug-coated balloon therapy predicting vessel restenosis at mid-term follow-up.

Chin Med J (Engl) 2021 Jun 4;134(12):1450-1456. Epub 2021 Jun 4.

Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, China.

Background: Drug-coated balloons (DCBs) have emerged as potential alternatives to drug-eluting stents in specific lesion subsets for de novo coronary lesions. Quantitative flow ratio (QFR) is a method based on the three-dimensional quantitative coronary angiography and contrast flow velocity during coronary angiography (CAG), obviating the need for an invasive fractional flow reserve procedural. This study aimed to assess the serial angiographic changes of de novo lesions post-DCB therapy and further explore the cut-off values of lesion and vessel QFR, which predict vessel restenosis (diameter stenosis [DS] ≥50%) at mid-term follow-up.

Methods: The data of patients who underwent DCB therapy between January 2014 and December 2019 from the multicenter hospital were retrospectively collected for QFR analysis. From their QFR performances, which were analyzed by CAG images at follow-up, we divided them into two groups: group A, showing target vessel DS ≥50%, and group B, showing target vessel DS <50%. The median follow-up time was 287 days in group A and 227 days in group B. We compared the clinical characteristics, parameters during DCB therapy, and QFR performances, which were analyzed by CAG images between the two groups, in need to explore the cut-off value of lesion/vessel QFR which can predict vessel restenosis. Student's t test was used for the comparison of normally distributed continuous data, Mann-Whitney U test for the comparison of non-normally distributed continuous data, and receiver operating characteristic (ROC) curves for the evaluation of QFR performance which can predict vessel restenosis (DS ≥50%) at mid-term follow-up using the area under the curve (AUC).

Results: A total of 112 patients with 112 target vessels were enrolled in this study. Group A had 41 patients, while group B had 71. Vessel QFR and lesion QFR were lower in group A than in group B post-DCB therapy, and the cut-off values of lesion QFR and vessel QFR in the ROC analysis to predict target vessel DS ≥50% post-DCB therapy were 0.905 (AUC, 0.741 [95% confidence interval, CI: 0.645, 0.837]; sensitivity, 0.817; specificity, 0.561; P < 0.001) and 0.890 (AUC, 0.796 [95% CI: 0.709, 0.882]; sensitivity, 0.746; specificity, 0.780; P < 0.001).

Conclusions: The cut-off values of lesion QFR and vessel QFR can assist in predicting the angiographic changes post-DCB therapy. When lesion/vessel QFR values are <0.905/0.890 post-DCB therapy, a higher risk of vessel restenosis is potentially predicted at follow-up.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/CM9.0000000000001577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213272PMC
June 2021

Chemical Ecology of the Asian Longhorn Beetle, Anoplophora glabripennis.

J Chem Ecol 2021 Jun 3;47(6):489-503. Epub 2021 Jun 3.

College of Environmental Science and Forestry, State University of New York, Syracuse, NY, 13210, USA.

The Asian longhorn beetle (ALB), Anoplophora glabripennis (Motschulsky), is a destructive forest pest in its native range, East Asia, or a high-risk invasive species in many other parts of the world. Extensive research has been directed toward the development of ALB management strategies. However, semiochemical-based trap lures, which are one of the effective tools for detecting, monitoring, and potentially assisting in eradicating cerambycids, have not reached operational efficacy for ALB to date, which is probably due to a grossly incomplete understanding of its chemical ecology. Here, we summarize the current progress in ALB chemical ecology including host selection and location, pheromone identification, trapping techniques, olfactory system, and related biology and behavior. We also briefly review the known semiochemicals in the subfamily Lamiinae, particularly the ALB congener, A. chinensis. Based on this knowledge, we highlight a potentially important role of some host-original chemicals, such as sesquiterpenes, in ALB host and mate location, and emphasize the basic studies on the biology and behavior of adult ALB. Last, we formulate suggestions for further research directions that may contribute to a better understanding of ALB chemical ecology and improved lure efficacy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10886-021-01280-zDOI Listing
June 2021
-->