Publications by authors named "Xu Teng"

260 Publications

Molecular Mechanism of the β-Lactamase Mediated β-Lactam Antibiotic Resistance of Isolated From a Chinese Teaching Hospital.

Front Microbiol 2022 28;13:855961. Epub 2022 Apr 28.

Department of Infectious Disease, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China.

can cause infections in the blood, lungs (pneumonia), or other parts of the body after surgery. To investigate the molecular characteristics of β-lactam antibiotic resistance of isolated from a hospital population between 2015 and 2017, in this study, the antimicrobial susceptibility and the resistance gene profile of the bacteria were determined. The Pulsed-field gel electrophoresis (PFGE) was used to characterize the clonal relatedness and sequencing and comparative genomic analysis were performed to analyze the structure of the resistance gene-related sequences. As a result, of the 260 . strains analyzed, the resistance rates for 6 β-lactam antibiotics ranged from 4.6 to 9.6%. A total of 7 genotypes of 44 β-lactamase genes were identified in 23 isolates (8.9%, 23/260). Four transconjugants from different donors carrying exhibited a phenotype of reduced susceptibility to piperacillin-tazobactam, ceftazidime, and cefepime, and 2 transconjugants harboring exhibited a phenotype of reduced susceptibility to carbapenems. positive isolates ( = 12) presented six PFGE patterns, designated groups A to F. Two genes ( and ) in PA1609 related to a class 1 integron (- ----) were encoded on a plasmid (pPA1609-475), while the gene of PA1616 also related to a class 1 integron was located on the chromosome. The results suggest that β-lactam antibiotic resistance and clonal dissemination exist in this hospital population. It indicates the necessity for molecular surveillance in tracking β-lactamase-producing strains and emphasizes the need for epidemiological monitoring.
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http://dx.doi.org/10.3389/fmicb.2022.855961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096163PMC
April 2022

RUNX2 recruits the NuRD(MTA1)/CRL4B complex to promote breast cancer progression and bone metastasis.

Cell Death Differ 2022 May 9. Epub 2022 May 9.

Key Laboratory of Cancer and Microbiome, State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

Runt-related transcription factor 2 (RUNX2) is an osteogenesis-related transcription factor that has emerged as a prominent transcription repressing factor in carcinogenesis. However, the role of RUNX2 in breast cancer metastasis remains poorly understood. Here, we show that RUNX2 recruits the metastasis-associated 1 (MTA1)/NuRD and the Cullin 4B (CUL4B)-Ring E3 ligase (CRL4B) complex to form a transcriptional-repressive complex, which catalyzes the histone deacetylation and ubiquitylation. Genome-wide analysis of the RUNX2/NuRD(MTA1)/CRL4B complex targets identified a cohort of genes including peroxisome proliferator-activated receptor alpha (PPARα) and superoxide dismutase 2 (SOD2), which are critically involved in cell growth, epithelial-to-mesenchymal transition (EMT) and invasion. We demonstrate that the RUNX2/NuRD(MTA1)/CRL4B complex promotes the proliferation, invasion, tumorigenesis, bone metastasis, cancer stemness of breast cancer in vitro and in vivo. Strikingly, RUNX2 expression is upregulated in multiple human carcinomas, including breast cancer. Our study suggests that RUNX2 is a promising potential target for the future treatment strategies of breast cancer.
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http://dx.doi.org/10.1038/s41418-022-01010-2DOI Listing
May 2022

Carcinoma-associated fibroblasts release microRNA-331-3p containing extracellular vesicles to exacerbate the development of pancreatic cancer the SCARA5-FAK axis.

Cancer Biol Ther 2022 Dec;23(1):378-392

Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.

microRNA-331-3p (miR-331-3p) has been displayed as an oncogene in pancreatic cancer (PC). The current research set out to elucidate how miR-331-3p in carcinoma-associated fibroblasts (CAFs)-derived extracellular vesicles (EVs) facilitated the tumorigenesis in PC. First, a dual-luciferase reporter assay was adopted to investigate the relationship between miR-331-3p and SCARA5. In addition, EVs were isolated normal fibroblasts and CAFs, and these isolated EVs were co-cultured with PC cells. Cell proliferative and migrating/invasive potentials were further evaluated with the help of a CCK-8 and Transwell assays, respectively. Gain- and loss-of-function assays were also implemented to assess the role of miR-331-3p, SCARA5, and FAK pathway in PC cells. Lastly, xenograft nude mice were established to investigate the role of miR-331-3p . miR-331-3p negatively targeted SCARA5 and was highly expressed in CAFs-derived EVs, which accelerated the proliferative, migrating, and invasive potentials of PC cells. Meanwhile, over-expression of miR-331-3p enhanced the proliferative, migrating, and invasive properties of PC cells and promoted tumor growth by manipulating SCARA5/FAK axis, whereas SCARA5 countered the oncogenic effects of miR-331-3p. Overall, miR-331-3p in CAFs-derived EVs inhibits SCARA5 expression and activates the FAK pathway, thereby augmenting the progression of PC. Our study provides a potential therapeutic target for the treatment of PC.
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http://dx.doi.org/10.1080/15384047.2022.2041961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090287PMC
December 2022

The convoluted process of diagnosing pulmonary mycosis caused by Exophiala dermatitidis: a case report.

BMC Infect Dis 2022 May 4;22(1):433. Epub 2022 May 4.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Xi Road, Guangzhou, 510120, Guangdong, China.

Background: Etiological diagnosis is a key step in the treatment of patients with rare pulmonary mycosis, and the lack of understanding of this disease and lack of specific markers for the detection of rare species, such as Exophiala dermatitidis, add to the difficulty in diagnosing the condition. Therefore, improving the diagnostic strategies for this disease is very important.

Case Presentation: A 52-year-old man presented with cough, sputum production and hemoptysis; chest computed tomography (CT) revealed multiple bilateral lesions. The pathogen was unable to be identified after three biopsies. Subsequently, we performed combined tissue metagenomic next-generation sequencing (mNGS). The results of mNGS and a good therapeutic response helped to identify the causative pathogen as Exophiala dermatitidis. Finally, the patient was diagnosed with Exophiala dermatitidis pneumonia.

Conclusions: Combining molecular techniques, such as mNGS, with clinical microbiological tests will improve the rate of positivity in the diagnosis of rare fungal infections, and the importance of follow-up should be emphasized.
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http://dx.doi.org/10.1186/s12879-022-07399-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069750PMC
May 2022

Hydrogen sulfide ameliorated preeclampsia via suppression of toll-like receptor 4-activated inflammation in the rostral ventrolateral medulla of rats.

Biomed Pharmacother 2022 Apr 26;150:113018. Epub 2022 Apr 26.

Department of Physiology, Hebei Medical University, Shijiazhuang, China; Hebei Collaborative Innovation Center for Cardio-Cerebrovascular Disease, Shijiazhuang, China. Electronic address:

This study aims to determine whether toll-like receptor 4 (TLR4)-mediated inflammation in rostral ventrolateral medulla (RVLM) causes sympathetic overactivity leading to preeclampsia (PE) and if TLR4 inhibition with hydrogen sulfide (HS) would reduce PE severity. Thirty patients with PE and 30 pregnant controls were involved. PE in rats was induced through deoxycorticosterone acetate and normal saline. NaHS (donor of HS), lipopolysaccharide (LPS) (TLR4 agonist), and TAK-242 (TLR4 inhibitor) were injected in lateral cerebral ventricle to investigate their effect on microglia-mediated inflammation in RVLM, sympathetic activation, and PE symptoms. In patients with PE, plasma levels of NE, TNF-α, and interleukin-1β were high compared with those of controls, whereas levels of HS were low. Rats with PE showed an increased amount of renal sympathetic nerve activity and plasma levels of NE, with decreased HS levels in RVLM. Microglia-mediated inflammation was observed in the RVLM of PE rats. Central infusion of LPS in pregnant rats induced microglia-mediated inflammation, sympathetic nervous tension, and PE-like symptoms, whereas TAK-242 reduced PE symptoms. NaHS treatment lessened microglia-mediated inflammation in the RVLM, sympathetic tension, and symptoms of PE both in PE rats and LPS-treating pregnant rats.These results suggest that inflammation in the RVLM caused by microglial activation might contribute to the progression of PE via an overactive sympathetic system. HS could reduce PE via inhibiting inflammation in the RVLM. These results might provide a new target for the treatment of PE.
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http://dx.doi.org/10.1016/j.biopha.2022.113018DOI Listing
April 2022

Effects of tannic acid on the transport behavior of trivalent chromium in soils and its mechanism.

Environ Pollut 2022 Jul 18;305:119328. Epub 2022 Apr 18.

National Engineering Research Center of Clean Technology in Leather Industry, Sichuan University, Chengdu 610065, China.

Trivalent chromium [Cr(III)] and tannins serve as necessary substances in leather processing and coexist in tannery site, which lead to the chromium contamination in site soil when disposed improperly. However, coexisting tannins are very likely to complex with Cr(III) and affect its properties, ultimately changing the mobility of chromium in soil. In this study, tannic acid (TA) was selected to investigate the complexation with Cr(III) and the influence on the solubility and sorption of Cr(III) in soils. Then, the transport behavior and mechanism of Cr(III)-TA complexes in soil was clarified. Dialysis results showed that the increase of TA concentration and solution pH promoted the formation of complexed Cr(III). The results of UV-Vis absorption spectroscopy, X-ray photoelectron spectroscopy, and density functional theory calculations indicated that the adjacent ionized phenolic hydroxyls in TA functioned as the binding sites with Cr(III) to form the Cr-O bonds and the degree of complexation increased with pH. The Cr(III)-TA complexes had higher solubility than free Cr(III) at pH ≥ 6.0. Batch sorption experiments demonstrated that the sorption capacity of Cr(III)-TA to soils with different pH was always lower than that of free Cr(III). These reasons led to the stronger mobility of Cr(III)-TA in soil columns than Cr(III). Our research reveals that the enhanced mobility of Cr(III) in soils coexisting with TA.
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http://dx.doi.org/10.1016/j.envpol.2022.119328DOI Listing
July 2022

Therapeutic endoscopy of a Dieulafoy lesion in a 10-year-old girl: A case report.

World J Clin Cases 2022 Feb;10(6):1966-1972

Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.

Background: There are multiple causes of sudden gastrointestinal bleeding in children. Reports of Dieulafoy lesions (DLs) in children are scarce. DLs can be fatal without appropriate treatment.

Case Summary: We present a retrospective analysis of the clinical manifestations, endoscopic features, and treatment of a Chinese girl with a DL, as well as a review of the relevant literature. A 10-year-old girl was admitted to our hospital with sudden massive hematemesis and melena. Abdominal computed tomography revealed suspected submucosal bleeding in the stomach. Finally, the disease was diagnosed with endoscopy due to the typical manifestations. We used electrocoagulation and hemoclips under endoscopy for hemostasis. No recurrence of hematemesis was identified during 4-wk' follow-up.

Conclusion: DLs in children are rare but an important cause of sudden gastrointestinal hemorrhage. Many pediatricians are inexperienced and often miss or delay diagnosis. Endoscopy as early as possible is the first choice for diagnosis and treatment.
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http://dx.doi.org/10.12998/wjcc.v10.i6.1966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891784PMC
February 2022

Metronidazole-resistant Clostridioides difficile: genomic and transcriptomic traits acquired under in-vitro metronidazole induction.

Int J Antimicrob Agents 2022 May 13;59(5):106570. Epub 2022 Mar 13.

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China; Key Laboratory of Clinical Pharmacology of Antibiotics, National Health and Family Planning Commission, Shanghai, China. Electronic address:

Decreased effectiveness of metronidazole for the treatment of Clostridioides difficile infection has been documented. One reason for this is that levels of metronidazole in the colon are generally low; therefore, a modest increase in the minimum inhibitory concentration of metronidazole for C. difficile may result in an insufficient therapeutic concentration. Due to the lack of efficient genetic manipulation tools for C. difficile strains, the resistance mechanism is largely unknown. In this study, a metronidazole-resistant strain (SH182IR) was acquired by in-vitro induction with metronidazole from a clinical metronidazole-heteroresistant strain (SH182), and the genomic and transcriptional changes were investigated through whole-genome sequencing and RNA-seq. The morphology of the two strains was studied by transmission electron microscopy, and the roles of drug efflux pumps in metronidazole resistance were determined by inhibition assay. Genomic analysis showed that the ferrous iron transporter feoB3 was truncated in SH182IR, indicating that feoB3 contributed to the metronidazole resistance of C. difficile. RNA-seq analysis showed that genes involved in peptidoglycan synthesis, efflux pumps and metronidazole reductive action were expressed differentially between the two strains. Further cell imaging confirmed that cell wall thickness was significantly greater in SH182IR. The efflux pump inhibitor test showed that addition of reserpine or cyanide 3-chlorophenylhydrazone reduced metronidazole resistance in SH182IR, thus proving the role of efflux pumps in metronidazole resistance. These results found an association between genomic variation and metronidazole resistance in C. difficile, and show that metronidazole resistance in C. difficile is multi-factorial, involving metronidazole metabolism, cell wall thickness and efflux pumps. These findings will help improve knowledge and understanding of metronidazole resistance of C. difficile.
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http://dx.doi.org/10.1016/j.ijantimicag.2022.106570DOI Listing
May 2022

Comparative Effects between Oral Lactoferrin and Ferrous Sulfate Supplementation on Iron-Deficiency Anemia: A Comprehensive Review and Meta-Analysis of Clinical Trials.

Nutrients 2022 Jan 27;14(3). Epub 2022 Jan 27.

Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China.

Ferrous sulfate is a commonly used iron supplement for the correction of iron-deficiency anemia but with frequent gastrointestinal side effects. Milk-derived iron-binding glycoprotein lactoferrin possesses well gastrointestinal tolerance and fewer side effects caused by the intake of high-dose iron. However, the underlying mechanism of the iron-enhancing effect of lactoferrin remains unclear. In addition, the comparative efficacies between lactoferrin and ferrous sulfate are also remained to be determined. We conducted a systematic review and meta-analysis on published intervention studies to investigate how lactoferrin modulate iron metabolism and evaluate the comparative effects between lactoferrin and ferrous sulfate supplementation on iron absorption, iron storage, erythropoiesis and inflammation. Lactoferrin supplementation had better effects on serum iron (WMD: 41.44 ug/dL; < 0.00001), ferritin (WMD: 13.60 ng/mL; = 0.003) and hemoglobin concentration (11.80 g/dL; < 0.00001), but a reducing effect on fractional iron absorption (WMD: -2.08%; = 0.02) and IL-6 levels (WMD: -45.59 pg/mL; < 0.00001) compared with ferrous sulfate. In conclusion, this study supports lactoferrin as a superior supplement to ferrous sulfate regarding the improvement in serum iron parameters and hemoglobin levels. Considering the weak influence of lactoferrin on iron absorption, the anti-inflammation effect of lactoferrin may be the potential mechanism to explain its efficacy on iron status and erythropoiesis.
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http://dx.doi.org/10.3390/nu14030543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838920PMC
January 2022

Reductive immobilization of Cr(VI) in contaminated water by tannic acid.

Chemosphere 2022 Jun 21;297:134081. Epub 2022 Feb 21.

Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China; National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China. Electronic address:

The rapid reductive immobilization of Cr(VI) from the aqueous solution was achieved by reduction to Cr(III) using tannic acid (TA), and subsequent pH-triggering precipitation of the organo-Cr(III) complexes formed in the redox reaction. The effects of TA concentration, temperature, and solution pH on the reduction of Cr(VI) were examined by batch experiments, and the rapid redox reduction followed a second-order kinetics with respect to Cr(VI) concentration in the pH range of 2.0-3.0. UV-visible spectra, FTIR, and XPS confirmed the complete detoxification of Cr(VI) concomitant with carboxylation of partial phenolic hydroxyls in TA. Synchronously, the reduced Cr(III) coordinated with carboxyl groups in oxidized TA (OTA) to form complexes, which exhibited remarkable pH-dependent size distribution characteristics as illustrated by SEM images and sequential filtration/ultrafiltration. The resulted Cr(III) complexes could aggregate into colloids with larger size and precipitate out at pH range of 6.0-8.0 via cross-linking, thereby leading to 93% Cr and 89% TOC immobilization. An eco-friendly and cost-effective method for Cr(VI) elimination and immobilization is provided because polyphenols are natural polymers derived from plants.
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http://dx.doi.org/10.1016/j.chemosphere.2022.134081DOI Listing
June 2022

The hub ten gene-based risk score system using RNA mA methylation regulator features and tumor immune microenvironment in breast cancer.

Breast Cancer 2022 Feb 16. Epub 2022 Feb 16.

Beijing Key Laboratory of Cancer Invasion and Metastasis Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China.

Background: RNA N-methyladenosine (mA) modification is primarily regulated by mA regulators, which play significant epigenetic regulatory roles in tumorigenesis, tumor development, and tumor immune microenvironment. However, the correlation between mA regulators and immune cell infiltration in breast cancer remains unclear.

Methods: In this study, mA modification patterns were evaluated based on 31 mA modification regulators. mA clusters were determined by consensus clustering. Immune landscape and immune cell infiltration subgroups were characterized by mA clusters. Key module and hub genes related to mA regulators and immune infiltration cells were identified by WGCNA. LASSO algorithm was applied to select prognostic signatures. Multivariate Cox regression analysis was applied to assess the prognostic value of gene signatures.

Results: Two distinct mA clusters were determined based on the expression of 31 mA modification regulators and characterized by two tumor immune microenvironment (TIME) immune cell infiltration subgroups. Further, a total of 1971 differentially expressed genes between breast cancer patients and healthy controls were screened, nine modules associated with clinical characteristics of breast cancer patients were identified. Later, one key module and 13 hub genes correlated with mA regulators and immune infiltration cells were identified. LASSO Cox regression analysis selected and constructed a ten-gene prognostic model to build a risk score system for individual breast cancer patient prognosis. The performance of the ten-gene-based risk score system was further validated in an independent dataset with an AUC of 0.659.

Conclusions: This study revealed that mA modification regulators played a significant role in the TIME regulation of breast cancer. The hub ten gene-based risk score system is valuable in predicting the prognosis of breast cancer patients, which may provide potential significance for breast cancer diagnosis, prognosis, and immunotherapy in the future.
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http://dx.doi.org/10.1007/s12282-022-01341-5DOI Listing
February 2022

Identification of Key Transcription Factors and Immune Infiltration Patterns Associated With Breast Cancer Prognosis Using WGCNA and Cox Regression Analysis.

Front Oncol 2021 21;11:742792. Epub 2021 Dec 21.

Beijing Key Laboratory of Cancer Invasion and Metastasis Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.

Breast cancer is the most frequently diagnosed cancer and the second leading cause of cancer death among women worldwide. Therefore, the need for effective breast cancer treatment is urgent. Transcription factors (TFs) directly participate in gene transcription, and their dysregulation plays a key role in breast cancer. Our study identified 459 differentially expressed TFs between tumor and normal samples from The Cancer Genome Atlas database. Based on gene expression analysis and weighted gene co-expression network analysis, the co-expression yellow module was found to be integral for breast cancer progression. A total of 121 genes in the yellow module were used for function enrichment. To further confirm prognosis-related TFs, COX regression and LASSO analyses were performed; consequently, a prognostic risk model was constructed, and its validity was verified. Ten prognosis-related TFs were identified according to their expression profile, survival probability, and target genes. COPS5, HDAC2, and NONO were recognized as hub TFs in breast cancer. These TFs were highly expressed in human breast cancer cell lines and clinical breast cancer samples; this result was consistent with the information from multiple databases. Immune infiltration analysis revealed that the proportions of resting dendritic and mast cells were greater in the low-risk group than those in the high-risk group. Thus, in this study, we identified three hub biomarkers related to breast cancer prognosis. The results provide a framework for the co-expression of TF modules and immune infiltration in breast cancer.
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http://dx.doi.org/10.3389/fonc.2021.742792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724129PMC
December 2021

Development and clinical implications of a novel CRISPR-based diagnostic test for pulmonary Aspergillus fumigatus infection.

J Microbiol Immunol Infect 2021 Dec 14. Epub 2021 Dec 14.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, National Center for Respiratory Medicine, Guangzhou 510120, China. Electronic address:

Background: Rapid and reliable diagnostic methods for Aspergillus fumigatus infection are urgently needed. Clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 13a (Cas13a) has high sensitivity and specificity in the diagnosis of viral infection. However, its potential use in detecting A. fumigatus remains unexplored. A highly sensitive and specific method using the CRISPR/Cas13a system was developed for the reliable and rapid detection of A. fumigatus.

Methods: The conserved internal transcribed spacer (ITS) region of A. fumigatus was used to design CRISPR-derived RNA (crRNA) and the corresponding recombinase polymerase amplification (RPA) primer sequence with the T7 promoter for the CRISPR assay. Twenty-five clinical isolates and 43 bronchoalveolar lavage fluid (BALF) remaining from routine examinations of patients with confirmed pulmonary aspergillosis were collected to further validate the CRISPR assay.

Results: No amplification signal was observed when genomic DNA from closely clinically related Aspergillus species, such as Aspergillus flavus, Aspergillus niger, and Aspergillus terreus, as well as Monascus purpureus Went and Escherichia coli, was tested by this assay, and the detection limit for A. fumigatus was 3 copies in a single reaction system. Validation experiments using the 25 clinical isolates demonstrated 91.7% specificity for the A. fumigatus section, and the sensitivity was 100% when first-generation sequencing was used as the standard. There was no significant difference between the PCR and CRISPR methods (P = 1.0), and the diagnosis results of the two methods were consistent (Kappa = 0.459, P = 0.003).

Conclusion: The study offers a new validated CRISPR/Cas13a technique for A. fumigatus detection, providing a simple, rapid and affordable test that is ready for application in the diagnosis of A. fumigatus infection.
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http://dx.doi.org/10.1016/j.jmii.2021.11.008DOI Listing
December 2021

Cytoplasmic eIF6 promotes OSCC malignant behavior through AKT pathway.

Cell Commun Signal 2021 12 18;19(1):121. Epub 2021 Dec 18.

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, No.1, Shanghai Road, Gulou District, Nanjing, Jiangsu, 210029, People's Republic of China.

Background: Eukaryotic translation initiation factor 6 (eIF6), also known as integrin β4 binding protein, is involved in ribosome formation and mRNA translation, acting as an anti-association factor. It is also essential for the growth and reproduction of cells, including tumor cells. Yet, its role in oral squamous cell carcinoma (OSCC) remains unclear.

Methods: The expression characteristics of eIF6 in 233 samples were comprehensively analyzed by immunohistochemical staining (IHC). Effects of eIF6 over-expression and knockdown on cell proliferation, migration and invasion were determined by CCK-8, wound healing and Transwell assays. Western blot, immunofluorescence (IF) and co-immunoprecipitation (co-IP) were performed for mechanical verification.

Results: We found that cytoplasmic eIF6 was abnormally highly expressed in OSCC tissues, and its expression was associated with tumor size and the clinical grade. Amplification of eIF6 promoted the growth, migration and invasion capabilities of OSCC cell lines in vitro and tumor growth in vivo. Through Western blot analysis, we further discovered that eIF6 significantly promotes epithelial-mesenchymal transformation (EMT) in OSCC cells, while depletion of eIF6 can reverse this process. Mechanistically, eIF6 promoted tumor progression by activating the AKT signaling pathway. By performing co-immunoprecipitation, we discovered a direct interaction between endogenous eIF6 and AKT protein in the cytoplasm.

Conclusion: These results demonstrated that eIF6 could be a new therapeutic target in OSCC, thus providing a new basis for the prognosis of OSCC patients in the future. Video abstract.
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http://dx.doi.org/10.1186/s12964-021-00800-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684100PMC
December 2021

CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.

Mol Cancer 2021 12 18;20(1):167. Epub 2021 Dec 18.

Department of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221006, Jiangsu, China.

Background: Accumulating studies have revealed that aberrant expression of circular RNAs (circRNAs) is widely involved in the tumorigenesis and progression of malignant cancers, including colorectal cancer (CRC). Nevertheless, the clinical significance, levels, features, biological function, and molecular mechanisms of novel circRNAs in CRC remain largely unexplored.

Methods: CRC-related circRNAs were identified through bioinformatics analysis and verified in clinical specimens by qRT-PCR and in situ hybridization (ISH). Then, in vitro and in vivo experiments were performed to determine the clinical significance of, functional roles of, and clinical characteristics associated with circIL4R in CRC specimens and cells. Mechanistically, RNA pull-down, fluorescence in situ hybridization (FISH), luciferase reporter, and ubiquitination assays were performed to confirm the underlying mechanism of circIL4R.

Results: CircIL4R was upregulated in CRC cell lines and in sera and tissues from CRC patients and was positively correlated with advanced clinicopathological features and poor prognosis. Functional experiments demonstrated that circIL4R promotes CRC cell proliferation, migration, and invasion via the PI3K/AKT signaling pathway. Mechanistically, circIL4R was regulated by TFAP2C and competitively interacted with miR-761 to enhance the expression of TRIM29, thereby targeting PHLPP1 for ubiquitin-mediated degradation to activate the PI3K/AKT signaling pathway and consequently facilitate CRC progression.

Conclusions: Our findings demonstrate that upregulation of circIL4R plays an oncogenic role in CRC progression and may serve as a promising diagnostic and prognostic biomarker for CRC detection and as a potential therapeutic target for CRC treatment.
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http://dx.doi.org/10.1186/s12943-021-01474-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684286PMC
December 2021

Survival stratification for colorectal cancer via multi-omics integration using an autoencoder-based model.

Exp Biol Med (Maywood) 2021 Dec 14:15353702211065010. Epub 2021 Dec 14.

Department of Gastrointestinal Surgery, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, PR China.

Prognosis stratification in colorectal cancer helps to address cancer heterogeneity and contributes to the improvement of tailored treatments for colorectal cancer patients. In this study, an autoencoder-based model was implemented to predict the prognosis of colorectal cancer via the integration of multi-omics data. DNA methylation, RNA-seq, and miRNA-seq data from The Cancer Genome Atlas (TCGA) database were integrated as input for the autoencoder, and 175 transformed features were produced. The survival-related features were used to cluster the samples using k-means clustering. The autoencoder-based strategy was compared to the principal component analysis (PCA)-, t-distributed random neighbor embedded (t-SNE)-, non-negative matrix factorization (NMF)-, or individual Cox proportional hazards (Cox-PH)-based strategies. Using the 175 transformed features, tumor samples were clustered into two groups (G1 and G2) with significantly different survival rates. The autoencoder-based strategy performed better at identifying survival-related features than the other transformation strategies. Further, the two survival groups were robustly validated using "hold-out" validation and five validation cohorts. Gene expression profiles, miRNA profiles, DNA methylation, and signaling pathway profiles varied from the poor prognosis group (G2) to the good prognosis group (G1). miRNA-mRNA networks were constructed using six differentially expressed miRNAs (let-7c, mir-34c, mir-133b, let-7e, mir-144, and mir-106a) and 19 predicted target genes. The autoencoder-based computational framework could distinguish good prognosis samples from bad prognosis samples and facilitate a better understanding of the molecular biology of colorectal cancer.
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http://dx.doi.org/10.1177/15353702211065010DOI Listing
December 2021

Multicenter assessment of shotgun metagenomics for pathogen detection.

EBioMedicine 2021 Dec 20;74:103649. Epub 2021 Nov 20.

Department of Infectious Diseases, Huashan Hospital affiliated to Fudan University, Shanghai 200040, China. Electronic address:

Background: Shotgun metagenomics has been used clinically for diagnosing infectious diseases. However, most technical assessments have been limited to individual sets of reference standards, experimental workflows, and laboratories.

Methods: A reference panel and performance metrics were designed and used to examine the performance of shotgun metagenomics at 17 laboratories in a coordinated collaborative study. We comprehensively assessed the reliability, key performance determinants, reproducibility, and quantitative potential.

Findings: Assay performance varied significantly across sites and microbial classes, with a read depth of 20 millions as a generally cost-efficient assay setting. Results of mapped reads by shotgun metagenomics could indicate relative and intra-site (but not absolute or inter-site) microbial abundance.

Interpretation: Assay performance was significantly impacted by the microbial type, the host context, and read depth, which emphasizes the importance of these factors when designing reference reagents and benchmarking studies. Across sites, workflows and platforms, false positive reporting and considerable site/library effects were common challenges to the assay's accuracy and quantifiability. Our study also suggested that laboratory-developed shotgun metagenomics tests for pathogen detection should aim to detect microbes at 500 CFU/mL (or copies/mL) in a clinically relevant host context (10^5 human cells/mL) within a 24h turn-around time, and with an efficient read depth of 20M.

Funding: This work was supported by National Science and Technology Major Project of China (2018ZX10102001).
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http://dx.doi.org/10.1016/j.ebiom.2021.103649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8608867PMC
December 2021

Clinical Evaluation of a Metagenomics-Based Assay for Pneumonia Management.

Front Microbiol 2021 16;12:751073. Epub 2021 Sep 16.

State Key Laboratory of Respiratory Disease, Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, Guangzhou, China.

Clinical value of metagenomic next-generation sequencing (mNGS) in pneumonia management is still controversial. A prospective study was conducted to evaluate the clinical impact of PneumoSeq in 57 immunocompetent (ICO) and 75 immunocompromised (ICH) pneumonia patients. The value of PneumoSeq for both etiological and clinical impact investigation in pneumonia was assessed. Among the 276 potential pathogens detected with PneumoSeq in our cohort, 251 (90.9%) were cross-validated. Clinical diagnoses of the causative pathogens were obtained for 97 patients, 90.7% of which were supported by PneumoSeq. Compared to conventional testing, PneumoSeq suggested potentially missed diagnoses in 16.7% of cases (22/132), involving 48 additional pathogenic microorganisms. In 58 (43.9%) cases, PneumoSeq data led to antimicrobial treatment de-escalation ( = 12 in ICO, = 18 in ICH) and targeted treatment initiation ( = 7 in ICO, = 21 in ICH). The PneumoSeq assay benefited the diagnosis and clinical management of both ICH and ICO pneumonia patients in real-world settings.
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http://dx.doi.org/10.3389/fmicb.2021.751073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481773PMC
September 2021

Identification and Characterization of a Novel Aminoglycoside 3''-Nucleotidyltransferase, ANT(3'')-IId, From .

Front Microbiol 2021 31;12:728216. Epub 2021 Aug 31.

Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.

A novel plasmid-encoded aminoglycoside 3''-nucleotidyltransferase ANT(3")-IId, was discovered in strain H7 isolated from a chick on an animal farm in Wenzhou, China. The whole-genome of H7 consisted of one chromosome and five plasmids (pH7-250, pH7-108, pH7-68, pH7-48, and pH7-11). was identified as being encoded on pH7-250, sharing the highest amino acid identity of 50.64% with a function-known resistance gene, (KB849358.1). Susceptibility testing and enzyme kinetic parameter analysis were conducted to determine the function of the aminoglycoside 3"-nucleotidyltransferase. The gene conferred resistance to spectinomycin and streptomycin [the minimum inhibitory concentration (MIC) levels of both increased 16-fold compared with the control strain]. Consistent with the MIC data, kinetic analysis revealed a narrow substrate profile including spectinomycin and streptomycin, with / ratios of 4.99 and 4.45×10M S, respectively. Sequencing analysis revealed that the gene was associated with insertion sequences (IS) element [ΔIS-ΔIS-hp-orf-orf-orf1-], and were identified in plasmids from various species. This study of the novel aminoglycoside 3"-nucleotidyltranferase ANT(3")-IId helps us further understand the functional and sequence characteristics of aminoglycoside 3"-nucleotidyltranferases, highlights the risk of resistance gene transfer among species and suggests that attention should be given to the emergence of new aminoglycoside 3"-nucleotidyltranferase genes.
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http://dx.doi.org/10.3389/fmicb.2021.728216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438517PMC
August 2021

The Antiviral Roles of Hydrogen Sulfide by Blocking the Interaction between SARS-CoV-2 and Its Potential Cell Surface Receptors.

Oxid Med Cell Longev 2021 3;2021:7866992. Epub 2021 Sep 3.

Department of Physiology, Hebei Medical University, Hebei 050017, China.

The ongoing coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is posing a great threat to the global economy and public health security. Together with the acknowledged angiotensin-converting enzyme 2, glucose-regulated protein 78, transferrin receptor, AXL, kidney injury molecule-1, and neuropilin 1 are also identified as potential receptors to mediate SARS-CoV-2 infection. Therefore, how to inhibit or delay the binding of SARS-CoV-2 with the abovementioned receptors is a key step for the prevention and treatment of COVID-19. As the third gasotransmitter, hydrogen sulfide (HS) plays an important role in many physiological and pathophysiological processes. Recently, survivors were reported to have significantly higher HS levels in COVID-19 patients, and mortality was significantly greater among patients with decreased HS levels. Considering that the beneficial role of HS against COVID-19 and COVID-19-induced comorbidities and multiorgan damage has been well-examined and reported in some excellent reviews, this review will discuss the recent findings on the potential receptors of SARS-CoV-2 and how HS modulates the above receptors, in turn blocking SARS-CoV-2 entry into host cells.
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http://dx.doi.org/10.1155/2021/7866992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421161PMC
September 2021

Development and Clinical Evaluation of a CRISPR-Based Diagnostic for Rapid Group B Streptococcus Screening.

Emerg Infect Dis 2021 09;27(9):2379-2388

Vertical transmission of group B Streptococcus (GBS) is among the leading causes of neonatal illness and death. Colonization with GBS usually is screened weeks before delivery during pregnancy, on the basis of which preventive measures, such as antibiotic prophylaxis, were taken. However, the accuracy of such an antenatal screening strategy has been questionable because of the intermittent nature of GBS carriage. We developed a simple-to-use, rapid, CRISPR-based assay for GBS detection. We conducted studies in a prospective cohort of 412 pregnant women and a retrospective validation cohort to evaluate its diagnostic performance. We demonstrated that CRISPR-GBS is highly sensitive and offered shorter turnaround times and lower instrument demands than PCR-based assays. This novel GBS test exhibited an overall improved diagnostic performance over culture and PCR-based assays and represents a novel diagnostic for potential rapid, point-of-care GBS screening.
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http://dx.doi.org/10.3201/eid2709.200091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386798PMC
September 2021

Identification of Three Clf-Sdr Subfamily Proteins in , and Comparative Genomics Analysis of a Locus Encoding CWA Proteins in Species.

Front Microbiol 2021 29;12:691087. Epub 2021 Jul 29.

The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China.

Coagulase-negative is an opportunistic pathogen that is capable of causing several infections, especially in patients with indwelling medical devices. Here, we determined the complete genome sequence of a clinical strain isolated from the blood culture of a 1-year-old nursling patient with acute upper respiratory infection. Genome-wide phylogenetic analysis confirmed the phylogenetic relationships between and other species. Using comparative genomics, we identified three cell wall-anchored (CWA) proteins at the same locus (), named SdrJ, SdrK, and SdrL, on the chromosome sequences of different strains. Structural predictions showed that SdrJ/K/L have structural features characteristic of Sdr proteins but exceptionally contained an unusual N-terminal repeat region. However, the C-terminal repetitive (R) region of SdrJ contains a significantly larger proportion of alanine (142/338, 42.01%) than the previously reported SdrI (37.00%). Investigation of the genetic organization revealed that the genes were always followed by one or two glycosyltransferase genes, and and were present in an ∼56 kb region bordered by a pair of 8 bp identical direct repeats, named Sw-Sdr. This region was further found to be located on a 160-kb region subtended by a pair of 160-bp direct repeats along with other virulence genes and resistance genes. Sw-Sdr contained a putative integrase that was probably a remnant of a functional integrase. Evidence suggests that Sw-Sdr is improbably an efficient pathogenicity island. A large-scale investigation of genomes showed that loci were a potential hotspot of insertion sequences (ISs), which could lead to intraspecific diversity at these loci. Our work expanded the repository of Sdr proteins, and for the first time, we established the connection between loci and phylogenetic relationships and compared the loci in different species, which provided large insights into the genetic environment of CWA genes in .
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http://dx.doi.org/10.3389/fmicb.2021.691087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360574PMC
July 2021

Lightweight cylindrical composite shell structures to support optical instruments in extremely large telescopes: A case study.

Sci Prog 2021 Jul-Sep;104(3):368504211036147

Nanjing Institute of Astronomical Optics and Technology, National Astronomical Observatories, CAS, Nanjing, China.

Aiming at the issues of heavy weight and insufficient structural performance of optical instrument supporting structures in extremely large telescopes, the Wide-Field Optical Spectrograph (WFOS) of the Thirty Meter Telescope (TMT) was taken as a case to study. In order to develop lightweight structures which satisfies the design requirements for mass and stiffness, a design scheme of cylindrical composite shells supporting structure was proposed and their finite element models were developed. A size optimisation and a ply sequence optimisation of the composite structure were carried out. The structures before and after optimisation were evaluated from the aspects of mass, displacement, failure index and fundamental frequency. After the optimised design, the mass of the optimised WFOS cylindrical composite shell structure is reduced to approximately 50%, but its maximum displacement (0.513 mm) and fundamental frequency (8.275 Hz) are nearly unchanged. The study indicates that a cylindrical composite shell structure is an efficient structural form for large optical instruments.
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http://dx.doi.org/10.1177/00368504211036147DOI Listing
August 2021

Identification and characteristics of a novel aminoglycoside phosphotransferase, APH(3')-IId, from an MDR clinical isolate of Brucella intermedia.

J Antimicrob Chemother 2021 10;76(11):2787-2794

Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.

Objectives: To describe a novel chromosomal aminoglycoside phosphotransferase named APH(3')-IId identified in an MDR Brucella intermedia ZJ499 isolate from a cancer patient.

Methods: Species identity was determined by PCR and MALDI-TOF MS analysis. WGS was performed to determine the genetic elements conferring antimicrobial resistance. Gene cloning, transcriptional analysis and targeted gene deletion, as well as protein purification and kinetic analysis, were performed to investigate the mechanism of resistance.

Results: APH(3')-IId consists of 266 amino acids and shares the highest identity (48.25%) with the previously known APH(3')-IIb. Expression of aph(3')-IId in Escherichia coli decreased susceptibility to kanamycin, neomycin, paromomycin and ribostamycin. The aph(3')-IId gene in ZJ499 was transcriptionally active under laboratory conditions and the relative abundance of this transcript was unaffected by treatment with the above four antibiotics. However, deletion of aph(3')-IId in ZJ499 results in decreased MICs of these drugs. The purified APH(3')-IId showed phosphotransferase activity against kanamycin, neomycin, paromomycin and ribostamycin, with catalytic efficiencies (kcat/Km) ranging from ∼105 to 107 M-1 s-1. Genetic environment and comparative genomic analyses suggested that aph(3')-IId is probably a ubiquitous gene in Brucella, with no mobile genetic elements detected in its surrounding region.

Conclusions: APH(3')-IId is a novel chromosomal aminoglycoside phosphotransferase and plays an important role in the resistance of B. intermedia ZJ499 to kanamycin, neomycin, paromomycin and ribostamycin. To the best of our knowledge, APH(3')-IId represents the fourth characterized example of an APH(3')-II enzyme.
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http://dx.doi.org/10.1093/jac/dkab272DOI Listing
October 2021

Prediction of prokaryotic transposases from protein features with machine learning approaches.

Microb Genom 2021 07;7(7)

Wenzhou Key Laboratory of Emergency, Critical Care, and Disaster Medicine, Department of Emergency, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, PR China.

Identification of prokaryotic transposases (Tnps) not only gives insight into the spread of antibiotic resistance and virulence but the process of DNA movement. This study aimed to develop a classifier for predicting Tnps in bacteria and archaea using machine learning (ML) approaches. We extracted a total of 2751 protein features from the training dataset including 14852 Tnps and 14852 controls, and selected 75 features as predictive signatures using the combined mutual information and least absolute shrinkage and selection operator algorithms. By aggregating these signatures, an ensemble classifier that integrated a collection of individual ML-based classifiers, was developed to identify Tnps. Further validation revealed that this classifier achieved good performance with an average AUC of 0.955, and met or exceeded other common methods. Based on this ensemble classifier, a stand-alone command-line tool designated TnpDiscovery was established to maximize the convenience for bioinformaticians and experimental researchers toward Tnp prediction. This study demonstrates the effectiveness of ML approaches in identifying Tnps, facilitating the discovery of novel Tnps in the future.
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http://dx.doi.org/10.1099/mgen.0.000611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477400PMC
July 2021

Experimental research on sintering construction spoil bricks based on microwave heating technology.

Environ Sci Pollut Res Int 2021 Dec 23;28(48):69367-69380. Epub 2021 Jul 23.

School of Civil Engineering, Xi'an University of Architecture & Technology, 710055, Xi'an, China.

The accumulation of construction spoil has brought great challenges to urban construction and environmental protection. It is an effective way to solve the problem of construction spoil accumulation by using construction spoil to sinter brick. At the same time, it can also reduce the waste of clay resources and farmland destruction caused by the production of sintered brick. Microwave sintering technology can greatly improve the sintering efficiency of materials. Sintering brick by microwave sintering technology can avoid a series of environmental problems in the traditional production process of sintered brick and can lead to an eco-friendly production of bricks. In this study, construction spoil was used as the raw material, and AlO, FeO, and TiO powders were studied as MASM for improving the temperature rise rate and sintering efficiency of the brick in microwave sintering process. The characteristics of construction spoil brick sintered by microwave were studied by series experiments including unit weight, compressive strength, and meso-analysis. Results proven that microwave sintering technology is an efficient technology for brick making. The MASM can effectively increase the temperature rise rate of the sample and simultaneously improve the impermeability of the sample and sintering efficiency.
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http://dx.doi.org/10.1007/s11356-021-15331-1DOI Listing
December 2021

BCAS3 exhibits oncogenic properties by promoting CRL4A-mediated ubiquitination of p53 in breast cancer.

Cell Prolif 2021 Aug 9;54(8):e13088. Epub 2021 Jul 9.

2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.

Objectives: Breast cancer-amplified sequence 3 (BCAS3) was initially found to be amplified in human breast cancer (BRCA); however, there has been little consensus on the functions of BCAS3 in breast tumours.

Materials And Methods: We analysed BCAS3 expression in BRCA using bio-information tools. Affinity purification and mass spectrometry were employed to identify BCAS3-associated proteins. GST pull-down and ubiquitination assays were performed to analyse the interaction mechanism between BCAS3/p53 and CUL4A-RING E3 ubiquitin ligase (CRL4A) complex. BCAS3 was knocked down individually or in combination with p53 in MCF-7 cells to further explore the biological functions of the BCAS3/p53 axis. The clinical values of BCAS3 for BRCA progression were evaluated via semiquantitative immunohistochemistry (IHC) analysis and Cox regression.

Results: We reported that the expression level of BCAS3 in BRCA was higher than that in adjacent normal tissues. High BCAS3 expression promoted growth, inhibited apoptosis and conferred chemoresistance in breast cancer cells. Mechanistically, BCAS3 overexpression fostered BRCA cell growth by interacting with the CRL4A complex and promoting ubiquitination and proteasomal degradation of p53. Furthermore, BCAS3 could regulate cell growth, apoptosis and chemoresistance through a p53-mediated mechanism. Clinically, BCAS3 overexpression was significantly correlated with a malignant phenotype. Moreover, higher expression of BCAS3 correlates with shorter overall survival (OS) in BRCA.

Conclusions: The functional characterization of BCAS3 offers new insights into the oncogenic properties and chemotherapy resistance in breast cancer.
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http://dx.doi.org/10.1111/cpr.13088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349660PMC
August 2021

Identification of Variants Associated With a Novel Tn-Like Integrative and Conjugative Element in Clinical Isolates.

Front Cell Infect Microbiol 2021 21;11:685068. Epub 2021 Jun 21.

The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China.

Florfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. is a key resistance gene that mediates resistance to florfenicol and could spread among different bacteria. Here, we investigated the prevalence of in 430 isolates from human clinical samples and identified three types of genes (designated , and ) in these isolates, with the most prevalent (5.3%, 23/430). FloR-T2 was a novel variant identified in this study, and exhibited less identity with other FloR proteins than FloRv. Moreover, and identified in strain TL1285 were functionally active and located on multi-drug resistance region of a novel incomplete Tn-like integrative and conjugative elements (ICE) in the chromosome. The expression of the two variants could be induced by florfenicol or chloramphenicol. These results indicated that the two variants played an essential role in the host's resistance to amphenicol and the spreading of these variants might be related with the Tn family ICE.
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http://dx.doi.org/10.3389/fcimb.2021.685068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256890PMC
July 2021

Characterization and identification of SFDC-1, a novel AmpC-type β-lactamase in Serratia fonticola.

Environ Microbiol 2021 12 16;23(12):7512-7522. Epub 2021 Jul 16.

The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, 325027, China.

The clinical and environmental infections caused by AmpC β-lactamases have been increasingly reported recently. In this study, we characterize the novel chromosome-encoded AmpC β-lactamase SFDC-1 identified in Serratia fonticola strain R28, which was isolated from a rabbit raised on a farm in southern China. SFDC-1 shared the highest amino acid identity of 79.6% with the functionally characterized AmpC β-lactamase gene bla , although it had highly homologous functionally uncharacterized relatives in the same species from different sources, including some of the clinical significance. The cloned bla exhibited resistance to a broad spectrum of β-lactam antibiotics, including most cephalosporins with the highest resistance to ampicillin, cefazolin and ceftazidime, with increased MIC levels ≥128-fold compared with the control strains. The purified SFDC-1 showed catalytic activities against β-lactams with the highest catalytic activity to cefazolin. The genetic context of bla and its relatives was conserved in the chromosome, and no mobile genetic elements were found surrounding them.
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http://dx.doi.org/10.1111/1462-2920.15663DOI Listing
December 2021

Bioinformatics analysis for the identification of key genes and long non-coding RNAs related to bone metastasis in breast cancer.

Aging (Albany NY) 2021 07 5;13(13):17302-17315. Epub 2021 Jul 5.

Department of Orthopaedics, Peking University Third Hospital, Beijing 100191, P.R. China.

The molecular mechanism of bone metastasis in breast cancer is largely unknown. Herein, we aimed to identify the key genes and long non-coding RNAs (lncRNAs) related to the bone metastasis of breast cancer using a bioinformatics approach. We screened differentially expressed genes and lncRNAs between normal breast and breast cancer bone metastasis samples using the GSE66206 dataset from the Gene Expression Omnibus. We also constructed a differentially expressed lncRNA-mRNA interaction network and analyzed the node degrees to identify the driving genes. After finding potential pathogenic modules of breast cancer bone metastasis, we identified breast cancer bone metastasis-related modules and functional enrichment analysis of the genes and lncRNAs in the modules. Based on the above analysis, we constructed a differentially expressed lncRNA-mRNA network related to bone metastasis in breast cancer and identified core driver genes, including and the lncRNA RP11-317-J19.1. The role of core driver genes and lncRNAs in the network implies their biological functions in regulating bone development and remodeling. Thus, targeting the core driver genes and lncRNAs in the network may be a promising therapeutic strategy to manage bone metastasis.
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http://dx.doi.org/10.18632/aging.203211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312419PMC
July 2021
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