Publications by authors named "Xu Su"

257 Publications

Variation and factors on heavy metal speciation during co-composting of rural sewage sludge and typical rural organic solid waste.

J Environ Manage 2022 Jan 6;306:114418. Epub 2022 Jan 6.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China. Electronic address:

In this study, a co-composting of rural organic solid waste (rural sewage sludge, kitchen waste and corn stalks) was conducted to analyze the variation of heavy metals (As, Cu, Cr, Ni, Pb, Hg, and Zn) and their major influencing factors. During composting, significant changes were observed in the total contents of heavy metals (p < 0.01): the total concentrations of As, Cu, Hg, Pb and Zn increased by 7.5%, 54.1%, 26.3%, 15.8%, and 34.2%, whereas that of Cr and Ni decreased by 71.3% and 33.4%, respectively. Heavy metals were mainly bound to the oxidizable and residual fractions. Spearman and Redundancy analysis (RDA) indicated that substances were significantly correlated with the changes in speciation of heavy metals, among all the factors, while pH and temperature were the dominating environmental influencing parameters. Several metal-resistant bacterial genera (Pseudomonas, Paenibacillus, Bacillus, Acinetobacter, Desulfovibrio, and Ochrobactrum, etc) were observed, with significant explanatory capacity for the changes in heavy metals. Composting showed a poor effect on heavy metal passivation, except for that of As. After composting, the heavy metal contents were consistent with the application standards. The evaluation of potential ecological risk showed a high cumulative ecological risk (336.9) of heavy metals. This study provides technical support and practical information for the disposal and safe recycling for rural organic solid waste.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jenvman.2021.114418DOI Listing
January 2022

Establishment of reference (housekeeping) genes via quantitative real-time PCR for investigation of the genomic basis of abiotic stress resistance in Psammochloa villosa (Poaceae).

J Plant Physiol 2022 Jan 20;268:153575. Epub 2021 Nov 20.

School of Life Sciences, Qinghai Normal University, Xining, 810008, China.

Psammochloa villosa is a desert plant growing in Northwest China with considerable resistance to abiotic stress, including drought, cold, and salt. To facilitate future studies of stress resistance in Psammochloa villosa, we sought to establish a suite of reference (or housekeeping) genes for utilization within future gene expression studies. Specifically, we selected nine candidate genes based on prior studies and new transcriptomic data for P. villosa, and we evaluated their expression stability in three different tissues of P. villosa under different treatments simulating abiotic stress conditions using four different bioinformatics assessments. Our results showed that TIP41 (TIP41-like family protein) was the most stable reference gene in drought- and salt-stressed leaves and salt-stressed stems, ELF-1α (elongation factor 1-α) was the most stable in cold-stressed leaves and drought- and salt-stressed roots, ACT (actin) was the most stable in drought-stressed stems, TUA (α-tubulin) was the most stable in cold-stressed stems, and 18S rRNA (18S ribosomal RNA) was the most stable in cold-stressed roots. Additionally, we tested the utility of these candidate reference genes to detect the expression pattern of P5CS (Δ-pyrroline-5-carboxylate synthetase), which is a drought-related gene. This study is the first report on selecting and validating reference genes of P. villosa under various stress conditions and will benefit future investigations of the genomic mechanisms of stress resistance in this ecologically important species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jplph.2021.153575DOI Listing
January 2022

Radiofrequency thermocoagulation for the treatment of trigeminal neuralgia.

Exp Ther Med 2022 Jan 30;23(1):17. Epub 2021 Oct 30.

Department of Neurosurgery, The Third Hospital of Jilin University and China-Japan Union Hospital, Changchun, Jilin 130033, P.R. China.

Although microvascular decompression (MVD) should be considered as the first-line treatment for classic trigeminal neuralgia (TN) owing to neurovascular compression of the trigeminal nerve, an increasing number of surgeons prefer radiofrequency thermocoagulation (RFT). RFT is a Gasserian ganglion-level ablative intervention that may achieve immediate pain relief for TN. It is used for emergency management when MVD is not suitable for the patient. As the gold surgical standard of classic trigeminal neuralgia, MVD has the advantage of longer efficacy. However, there are currently no high-quality controlled trials to evaluate the efficacy of MVD and RFT. For the present systematic review, the PubMed, Embase and Cochrane databases (all entries up until July 31, 2020) were searched to identify studies related to RFT in order to provide valuable information for clinical decision-making. The efficacy of the RFT method was evaluated in terms of the initial pain relief percentage, recurrence rate and follow-up time. Furthermore, the incidence rate of various postoperative complications was retrieved. RFT was used for a wider range of applications than MVD, including use for primary (owing to neurovascular compression of the trigeminal nerve), idiopathic and secondary (due to primary neurological diseases) TN, and provided a high rate of initial pain relief and long-term pain control. Although this method has several side effects, the incidence of complications could be reduced by precise cannulation. Furthermore, the complications that occurred were not permanent. Thus, RFT is a safe and effective minimally invasive method of pain relief for patients with TN.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/etm.2021.10939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593925PMC
January 2022

Preparation and disinfection properties of graphene oxide/trichloroisocyanuric acid disinfectant.

Nanotechnology 2021 Dec 23;33(11). Epub 2021 Dec 23.

School of Materials Science and Engineering, Lanzhou University of Technology, Langongping Road, Lanzhou 730050, Gansu Province, People's Republic of China.

Due to the impact of the new crown epidemic in recent years, disinfectants have played an increasingly important role, so the research and development of new high-efficiency nano-disinfectants are urgent issues. In this study, graphene oxide (GO) was first prepared by the modified Hummer method. Then, the GO/trichloroisocyanuric acid (TCCA) composite was prepared by loading TCCA into GO with the blending method. X-ray diffraction, scanning electron microscopy, transmission electron microscopy, x-ray photoelectron spectroscopy and atomic force microscopy were used to characterize the composite. The results showed that TCCA was successfully loaded on the surface of GO or intercalated among GO layers. Next, the antibacterial performance of the composite againstandwas tested by the 96-well plate assay. A bactericidal kinetic curve, bacterial inhibition tests, and the mechanism of bacterial inhibition were discussed. The results showed that the minimum inhibitory concentration (MIC) of the GO/TCCA composite (GO:TCCA ratio = 1:50) was 327.5g mlagainstand 655g mlagainst. At the MIC, the inhibition rate of the GO/TCCA composite exceeded 99.46% againstand 99.17% against. The bactericidal kinetic curves indicate that the GO/TCCA composite has an excellent bactericidal effect againstand.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ac3b82DOI Listing
December 2021

Comprehensive Analysis of the Mechanism of Periodontitis-Related mRNA Expression Combined with Upstream Methylation and ceRNA Regulation.

Genet Test Mol Biomarkers 2021 Nov;25(11):707-719

Department of Stomatology, Xinjiang Uygur Autonomous Region People's Hospital, Urumqi, China.

Periodontitis is a multifactorial disease mainly caused by the formation of plaque biofilm, which can lead to the gradual destruction of tooth-supporting tissues. Current research on the genetics and epigenetics of periodontitis remains relatively limited, and the molecular mechanisms remain largely unknown. Our aims were to construct competitive endogenous RNA (ceRNA) network and determine DNA methylation patterns of target genes to help elucidate the pathogenesis of periodontitis. We analyzed the expression profiles of the GSE16134, GSE54710, GSE10334, and GSE59932 datasets from the Gene Expression Omnibus database through the weighted gene coexpression network analysis system and screened mRNAs that are regulated by the level of methylation and are associated with the occurrence of periodontitis. Next, a lncRNA-miRNA-mRNA ceRNA network was constructed using databases including miRanda and TargetScan. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were conducted for genes in the clinically significant modules. Finally, a protein-protein interaction network was built. We finally identified four mRNAs, four miRNAs, and six lncRNAs as shared differentially expressed genes related to the periodontitis inflammation pathway. , , , and were identified as key genes whose expression was significantly enriched in the nuclear factor κB and TLR4 pathways. Moreover, the expression of 28 genes were downregulated by hypermethylation and 70 genes were upregulated by hypomethylation. The constructed ceRNA network can improve our understanding of the pathogenesis of periodontitis. Candidate mRNAs from the ceRNA network could serve as new therapeutic targets and prognostic biomarkers in periodontitis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/gtmb.2021.0090DOI Listing
November 2021

Verification of Acetylation of Trichodermol to Trichodermin in the Plant Endophyte .

Front Microbiol 2021 25;12:731425. Epub 2021 Oct 25.

Institute of Biotechnology, Zhejiang University, Hangzhou, China.

Trichodermin, a trichothecene first isolated in species, is a sesquiterpenoid antibiotic that exhibits significant inhibitory activity to the growth of many pathogenic fungi such as , , and by inhibiting the peptidyl transferase involved in eukaryotic protein synthesis. Trichodermin has also been shown to selectively induce cell apoptosis in several cancer cell lines and thus can act as a potential lead compound for developing anticancer therapeutics. The biosynthetic pathway of trichodermin in has been identified, and most of the involved genes have been functionally characterized. An exception is , which encodes a putative acetyltransferase. Here, we report the identification of a gene cluster that contains seven genes expectedly involved in trichodermin biosynthesis (, , , , , , and ) in the trichodermin-producing endophytic fungus . As in , is not included in the cluster. Functional analysis provides evidence that TRI3 acetylates trichodermol, the immediate precursor, to trichodermin. Disruption of gene eliminated the inhibition to by culture filtrates and significantly reduced the production of trichodermin but not of trichodermol. Both the inhibitory activity and the trichodermin production were restored when native gene was reintroduced into the disruption mutant. Furthermore, a His-tag-purified TRI3 protein, expressed in , was able to convert trichodermol to trichodermin in the presence of acetyl-CoA. The disruption of also resulted in lowered expression of both the upstream biosynthesis genes and the regulator genes. Our data demonstrate that encodes an acetyltransferase that catalyzes the esterification of the C-4 oxygen atom on trichodermol and thus plays an essential role in trichodermin biosynthesis in this fungus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.731425DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573352PMC
October 2021

The pilot of a new patient classification-based payment system in China: The impact on costs, length of stay and quality.

Soc Sci Med 2021 11 20;289:114415. Epub 2021 Sep 20.

School of Public Health, Fudan University, Shanghai, China; Key Laboratory of Health Technology Assessment (Fudan University), Ministry of Health, Shanghai, China. Electronic address:

With the urgent need to regulate provider behaviors, China developed a novel patient classification with global budget payment system, expecting to achieve both easy implementation and cost containment. The new system, called "diagnosis-intervention packet (DIP)" payment, is based on a deterministic patient classification approach, which groups patients according to the combination of principal diagnosis ICD-10 (International Classification of Diseases, 10th Revision) codes and procedure ICD-9-CM3 (International Classification of Diseases, 9th Revision, Clinical Modification) codes and links each group to relative historical costs market-wide. This study investigated the impact of the DIP-based payment on inpatient costs, length of stay, and quality of care in the largest DIP pilot city of China. In 2018, the city changed from the "fixed rate per admission with a cap on annual total compensation" policy to DIP with global budget for all insured inpatients. A difference-in-differences approach was employed to identify changes in outcome variables before and after the DIP policy among insured relative to uninsured patients. We found an average of 8.5% (p = 0.000) increase in inpatient costs per case (as intended), trivial changes in length of stay, and a 3.6% (p = 0.046) reduction in postoperative complication rate in response to DIP adoption among patients with high severity. Our findings suggested that the DIP-based payment helped regulate provider behaviors when treating high-risk patients. And the new payment has the potential for rapid rollout in resource-limited areas where lack a uniform coding practice or high-quality historical data.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.socscimed.2021.114415DOI Listing
November 2021

M1 macrophages-derived exosomes miR-34c-5p regulates interstitial cells of Cajal through targeting SCF.

J Biosci 2021 ;46

Department of Anorectal Surgery, Yancheng Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Yancheng 224001, Jiangsu, China.

Slow transit constipation (STC) is a gastrointestinal disorder characterized by abnormal prolonged colonic transit time, which affects the life quality of many people. The decrease number of interstitial cells of Cajal (ICCs) is involved in the pathogenesis of STC. However, the molecular mechanism of loss of ICCs in STC remains unclear, making it difficult to develop new agents for the disease. In this study, we investigated the mechanism of decreasing ICCs in the pathogenesis of STC. We constructed the STC model rats by using atropine and diphenoxylate. A series of methods were used including immunofluorescence and immunochemistry staining, western blot, qRT-PCR, exosomes extraction and exosomes labeling. The results indicate that ICCs decreased in the STC rats accompanied with the macrophages activation. Further studies suggested that macrophages decreased the cell viability of ICCs by secretion exosomes containing miR-34c-5p. miR-34c5p targeted the 3Ꞌ -UTR of stem cell factor(SCF) mRNA and regulated the expression of SCF negatively. In conclusion, we demonstrated a novel regulatory mechanism of ICCs cell viability in STC. We found that exosome miR-34c-5p mediate macrophage-ICCs cross-talk. M1 macrophages derived exosomes miR-34c-5p decreased ICCs cell viability by directly targeting SCF.
View Article and Find Full Text PDF

Download full-text PDF

Source
January 2022

Non-targeted analysis of vulgarisins by using collisional dissociation mass spectrometry for the discovery of analogues from Prunella vulgaris.

Anal Bioanal Chem 2021 Nov 3;413(26):6513-6521. Epub 2021 Sep 3.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, Guizhou, China.

Vulgarisins are members of diterpenoids with rare 5/6/4/5 ring skeleton from Prunella vulgaris Linn. (P. vulgaris). Their molecular scaffolds comprise different hydroxylation and degree of esterification. Vulgarisins have attracted many attentions in the fields of food and medicine for their potent bioactivities. Firstly, four reference compounds were analyzed by higher-energy collisional dissociation mass spectrometry (HCD MS/MS) and the fragmentation patterns for molecular scaffold were summarized. And then, a high-performance liquid chromatography/electrospray ionization/high-resolution mass spectrometry (HPLC-ESI-HR-MS) method was adopted to investigate the P. vulgaris extracts. Finally, the proposed analysis results were successfully applied to facilitate the discovery of the vulgarisins analogues from P. vulgaris. For the four reference compounds, the sodium adduct was the predominate ion in full scan. A specific fragmentation pathway of [M+Na] ions leads to produce diagnostic ions of vulgarisins at m/z 325 under HCD, which was formed through consecutive-side chains lost. Twenty-three diterpenoids, including 18 vulgarisins analogues, were identified or tentatively characterized in the botanical extracts of P. vulgaris based on their elemental constituents and characteristic fragment ion profiles. Two new vulgarisins analogues in the plant were isolated and their structures were illustrated based on extensive spectroscopic analysis using D and D nuclear magnetic resonance (NMR) spectroscopy. The HCD MS/MS method, including the profiles of the diagnostic ions induced by characteristic fragmentation, is an effective technique for the discovery of vulgarisins analogues in P. vulgaris. The expected fragmentation pattern knowledge will also facilitate the analysis of other natural products.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00216-021-03615-xDOI Listing
November 2021

The role of multiple metabolic genes in predicting the overall survival of colorectal cancer: A study based on TCGA and GEO databases.

PLoS One 2021 16;16(8):e0251323. Epub 2021 Aug 16.

Department of Gastroenterology, First Affiliated Hospital of Bengbu Medical College, Bengbu, China.

The recent advances in gene chip technology have led to the identification of multiple metabolism-related genes that are closely associated with colorectal cancer (CRC). Nevertheless, none of these genes could accurately diagnose or predict CRC. The prognosis of CRC has been made by previous prognostic models constructed by using multiple genes, however, the predictive function of multi-gene prognostic models using metabolic genes for the CRC prognosis remains unexplored. In this study, we used the TCGA-CRC cohort as the test dataset and the GSE39582 cohort as the experimental dataset. Firstly, we constructed a prognostic model using metabolic genes from the TCGA-CRC cohort, which were also associated with CRC prognosis. We analyzed the advantages of the prognostic model in the prognosis of CRC and its regulatory mechanism of the genes associated with the model. Secondly, the outcome of the TCGA-CRC cohort analysis was validated using the GSE39582 cohort. We found that the prognostic model can be employed as an independent prognostic risk factor for estimating the CRC survival rate. Besides, compared with traditional clinical pathology, it can precisely predict CRC prognosis as well. The high-risk group of the prognostic model showed a substantially lower survival rate as compared to the low-risk group. In addition, gene enrichment analysis of metabolic genes showed that genes in the prognostic model are enriched in metabolism and cancer-related pathways, which may explain its underlying mechanism. Our study identified a novel metabolic profile containing 11 genes for prognostic prediction of CRC. The prognostic model may unravel the imbalanced metabolic microenvironment, and it might promote the development of biomarkers for predicting treatment response and streamlining metabolic therapy in CRC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251323PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367004PMC
November 2021

SARS-CoV-2 Genomic Sequencing Revealed N501Y and L452R Mutants of S/A Lineage in Tianjin Municipality, China.

Virol Sin 2021 10 11;36(5):1228-1231. Epub 2021 Aug 11.

Department of Pathogenic Microbiology, Tianjin Centers for Disease Control and Prevention, Tianjin, 300011, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12250-021-00432-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8356212PMC
October 2021

Population genetic structure and evolutionary history of (Trin.) Bor (Poaceae) revealed by AFLP marker.

Ecol Evol 2021 Aug 13;11(15):10258-10276. Epub 2021 Jul 13.

School of Geosciences Qinghai Normal University Xining China.

is an ecologically important desert grass that occurs in the Inner Mongolian Plateau where it is frequently the dominant species and is involved in sand stabilization and wind breaking. We sought to generate a preliminary demographic framework for to support the future studies of this species, its conservation, and sustainable utilization. To accomplish this, we characterized the genetic diversity and structure of 210 individuals from 43 natural populations of using amplified fragment length polymorphism (AFLP) markers. We obtained 1,728 well-defined amplified bands from eight pairs of primers, of which 1,654 bands (95.7%) were polymorphic. Results obtained from the AFLPs suggested effective alleles among populations of 1.32, a Nei's standard genetic distance value of 0.206, a Shannon index of 0.332, a coefficient of gene differentiation ( ) of 0.469, and a gene flow parameter (m) of 0.576. All these values indicate that there is abundant genetic diversity in , but limited gene flow. An analysis of molecular variance (AMOVA) showed that genetic variation mainly exists within populations (64.2%), and we found that the most genetically similar populations were often not geographically adjacent. Thus, this suggests that the mechanisms of gene flow are surprisingly complex in this species and may occur over long distances. In addition, we predicted the distribution dynamics of based on the spatial distribution modeling and found that its range has contracted continuously since the last interglacial period. We speculate that dry, cold climates have been critical in determining the geographic distribution of during the Quaternary period. Our study provides new insights into the population genetics and evolutionary history of in the Inner Mongolian Plateau and provides a resource that can be used to design in situ conservation actions and prioritize sustainable utilization.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ece3.7831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328423PMC
August 2021

Reconfigurable meta-radiator based on flexible mechanically controlled current distribution in three-dimensional space.

Opt Lett 2021 Aug;46(15):3633-3636

In this paper, we provide an experimental proof-of-concept of this dynamic three-dimensional (3D) current manipulation through a 3D-printed reconfigurable meta-radiator with periodically slotted current elements. By utilizing the working frequency and the mechanical configuration comprehensively, the radiation pattern can be switched among 12 states. Inspired by maximum likelihood method in digital communications, a robustness-analysis method is proposed to evaluate the potential error ratio between ideal cases and practice. Our work provides a previously unidentified model for next-generation information distribution and terahertz-infrared wireless communications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OL.430318DOI Listing
August 2021

Identification of differentially expressed microRNAs under imidacloprid exposure in Sitobion miscanthi.

Pestic Biochem Physiol 2021 Aug 5;177:104885. Epub 2021 Jun 5.

College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, PR China.

Imidacloprid is a neonicotinoid that targets sucking pests, such as aphids and the green leaf bug and has been widely applied in wheat fields to control wheat aphids in China. To investigate the involvement of miRNAs in imidacloprid resistance, we sequenced small RNA libraries of Sitobion miscanthi Fabricius, across two different treatments using Illumina short-read sequencing technology. As a result, 265 microRNAs (miRNAs), of which 242 were known and 23 were novel, were identified. Quantitative analysis of miRNA levels showed that 23 miRNAs were significantly up-regulated, and 54 miRNAs were significantly down-regulated in the nymphs of S. miscanthi treated with imidacloprid in comparison with those of the control. Modulation of the abundances of differentially expressed miRNAs, smi-miR-316, smi-miR-1000, and smi-miR-iab-4 by the addition of the corresponding antagomir/inhibitor to the artificial diet significantly changed the susceptibility of S. miscanthi to imidacloprid. Subsequently, the post-transcriptional regulatory mechanism was conducted, smi-miR-278 and smi-miR-316 were confirmed to be participated in the post-transcriptional regulation of nAChRα1A and CYP4CJ6, respectively. The results suggested that miRNAs differentially expressed in response to imidacloprid could play a critical regulatory role in the metabolism of S. miscanthi to imidacloprid.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pestbp.2021.104885DOI Listing
August 2021

Growing phosphorus dilemma: The opportunity from aquatic systems' secondary phosphorus retention capacity.

Sci Total Environ 2021 Nov 8;796:148938. Epub 2021 Jul 8.

Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799, Jimei Road, Xiamen 361021, China; Xiamen Key Lab of Urban Metabolism, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China. Electronic address:

The essential cause of phosphorus scarcity and phosphorus-induced risks, i.e. phosphorus dilemma, mainly lies in current low phosphorus flow efficiency (PFE) in agricultural systems. Improving PFE largely depends on secondary phosphorus retention along the phosphorus flow chain from phosphate mining to terrestrial agricultural systems, to aquatic systems, and ultimately to seabed deposition. Our review found that aquatic systems will have the opportunity and growing capacity to retain seaward secondary phosphorus carried by the runoff, due to its location between land and water systems, its ability of converting secondary phosphorus from both land and aquatic systems into aquatic products, and its rapid expansion with low PFE. However, a knowledge gap exists in secondary phosphorus retention in aquatic systems compared to in terrestrial systems. Although the phosphorus retention literature continues to grow in environmental and agricultural & biological sciences, only 8.8% of the documents are related to aquatic systems with few quantification studies. Based on the literature with phosphorus retention quantification since 1979, we divided the reported phosphorus interceptors into abiotic and biotic groups, further into 7 categories and more subcategories. By 2020, eight categories of interceptors had been reported, increased from only one interceptor in 1979. However, most of them focused on wetlands, only a few studies on aquatic organisms which concentrated in 8 countries before 2000. Thus, it is urgent to emphasize aquatic systems' secondary phosphorus retention capacity and its systemic benefits for a sustainable phosphorus use.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.148938DOI Listing
November 2021

Mercury uptake by Paspalum distichum L. in relation to the mercury distribution pattern in rhizosphere soil.

Environ Sci Pollut Res Int 2021 Dec 9;28(47):66990-66997. Epub 2021 Jul 9.

School of Environmental and Resource Sciences, Zhejiang A&F University, Lin'an, 311300, Zhejiang Province, China.

Paspalum distichum L. was tested to evaluate their phytoremediation capacity for Hg contaminated soil through analyzing the dissipation of Hg in soil through a greenhouse study by using self-made rhizos box. Original soil samples were collected at Hg mining site with serious Hg contamination and a control site, respectively. Planting of P. distichum. L last for 60 days. Soil and plant samples were collected from four periods (0 d, 20 d, 40 d, and 60 d) and soil samples were collected from five different rhizosphere distance in horizontal direction (0-2 cm, 2-4cm, 4-6cm, 6-8cm, 8-10cm). The results showed that the presence of P. distichum. L significantly accelerated the Hg dissipation in soil compared with control. Hg concentration in the rhizospheric soil was affected by the plant growth period and the distance to the plant roots. The closer of soil to the root of P. distichum. L, the lower mercury concentration in soil. During the 60-day growing period, the concentrations of total Hg (THg) and methylmercury (MeHg) reduced by 45% and 64%, respectively, in the rhizosphere (0-2cm) of Hg contaminated soil. However, MeHg concentration was increased near the roots (0-4 cm) during the initial growing period (0-20 d), which may be attributed to the influence of root exudates. Root is the major part for Hg accumulation in P. distichum. L. The low ratio between Hg concentrations in underground and aboveground tissues indicated that it seemed difficult for Hg translocation from root to shoot. The highest THg (9.71 ± 3.09 μg·g) and MeHg (26.97 ± 0.98 ng·g) value in root of P. distichum. L were observed at the 20 day when P. distichum. L grown in Hg contaminated soil. The results of chemical fractions analyses showed that elemental Hg and residual Hg were the two major speciations followed by organic bound Hg in the Hg contaminated soil, which indicated the high bioavailability and ecological potential risk of Hg in Hg contaminated soil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-15093-wDOI Listing
December 2021

Novel method using DW-MRI and ADC images to guide stereotactic biopsy for the diagnosis small primary angiitis of the central nervous system: a case report.

Eur J Med Res 2021 Jun 23;26(1):58. Epub 2021 Jun 23.

Department of Neurosurgery, China-Japan Union Hospital of Jilin University, No. 126, Xiantai Street, Changchun, 130033, China.

Objective: To determine the role of diffusion-weighted magnetic resonance imaging (DW-MRI) and apparent diffusion coefficient (ADC) imaging to guide stereotactic biopsy for the diagnosis of intracranial angiitis.

Case Presentation: In a 28-year-old woman who had experienced inactive headache and right limbs numbness for 4 days, preoperative magnetic resonance (MR) scanning, enhanced scanning, diffusion tensor imaging, magnetic resonance spectroscopy, diffusion-weighted imaging (DWI), and ADC image scanning were performed. Stereotactic biopsy was performed in one target where the area of edema detected with MR FLAIR, and two targets where the area shown as a high-value and a lower value area in the DWI/ADC image. Pathological examinations together with computed tomographic and enhanced MRI scans were conducted after surgery. A preoperative enhanced MRI scan showed a uniform low-intensity lesion in the patient's left centrum semiovale, with a volume of 3.1 cm. The DWI and ADC images showed uneven high-intensity signals and different ADC values in the lesion area, respectively. During surgery, tissues around the lesion and the lesion center were sampled at the three selected targets. The postoperative pathological diagnosis was primary angiitis of the central nervous system, and the patient was given anti-inflammatory medication and hormone therapy. The 3-year follow-up confirmed that the patient had recovered well, with a Glasgow Outcome Scale score of five.

Conclusion: DW-MRI and ADC images can be reliably used to determine the location of small intracranial lesions, and guide stereotactic biopsy to facilitate the diagnosis of primary vasculitis of the central nervous system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40001-021-00529-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220829PMC
June 2021

Opposite Growth Responses of and to Photoperiods and Temperatures.

Plants (Basel) 2021 May 25;10(6). Epub 2021 May 25.

Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510160, China.

Shift of phytoplankton niches from low to high latitudes has altered their experienced light exposure durations and temperatures. To explore this interactive effect, the growth, physiology, and cell compositions of smaller and larger , globally distributed toxic red tide dinoflagellates, were studied under a matrix of photoperiods (light:dark cycles of 8:16, 16:8, and 24:0) and temperatures (18 °C, 22 °C, 25 °C, and 28 °C). Under continuous growth light condition (L:D 24:0), the growth rate (µ) of small increased from low to medium temperature, then decreased to high temperature, while the µ of large continuously decreased with increasing temperatures. Shortened photoperiods reduced the µ of , but enhanced that of . As temperature increased, cellular Chl content increased in both and , while the temperature-induced effect on RubisCO content was limited. Shortened photoperiods enhanced the Chl but reduced RubisCO contents across temperatures. Moreover, shortened photoperiods enhanced photosynthetic capacities of both and i.e., promoting the PSII photochemical quantum yield (F/F, Φ), saturation irradiance (E), and maximum relative electron transfer rate (rETRmax). Shortened photoperiods also enhanced dark respiration of across temperatures, but reduced that of , as well as the antioxidant activities of both species. Overall, and showed differential growth responses to photoperiods across temperatures, probably with cell size.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/plants10061056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229041PMC
May 2021

The effect of PCSK9 inhibitors on brain stroke prevention: A systematic review and meta-analysis.

Nutr Metab Cardiovasc Dis 2021 07 20;31(8):2234-2243. Epub 2021 Apr 20.

Neurology Department, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 05000, China. Electronic address:

Background And Aims: Although proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors have been shown to improve cardiovascular outcomes, their effects on brain stroke risk are unclear. The present meta-analysis aimed to evaluate the effects of PCSK9 inhibitors on brain stroke prevention.

Methods And Results: We searched PubMed, Embase, Cochrane Library, Web of Science, and ClinicalTrials.gov for research published until December 30, 2020, to find randomized controlled trials (RCTs) of PCSK9 inhibitors for brain stroke prevention. Relative risk (RR) and 95% confidence intervals (CIs) were used to represent the outcomes. Seven RCTs with 57,440 participants, including 29,850 patients treated with PCSK9 inhibitors and 27,590 control participants, were included. PCSK9 inhibitors were associated with significant reductions in total brain stroke risk (RR, 0.77; 95% CI, 0.67-0.88; P < 0.001) and ischemic brain stroke risk (RR, 0.76; 95% CI, 0.66, 0.89; P < 0.001) in comparison with the control group. There was no significant difference in cardiovascular mortality (RR, 0.95; 95% CI, 0.84-1.07; P = 0.382) and the risk of hemorrhagic brain stroke (RR, 1.00; 95% CI, 0.66-1.51; P = 0.999) between patients treated with PCSK9 inhibitors and controls. PCSK9 inhibitors did not significantly increase the incidence of neurocognitive adverse events (RR, 1.02; 95% CI, 0.81-1.29; P = 0.85). Moreover, subgroup analysis showed no difference in cognitive function disorder risks among different PCSK9 inhibitors and treatment times.

Conclusions: PCSK9 inhibitors significantly reduced the risk of total brain stroke and ischemic brain stroke without increasing the risk of brain hemorrhage and neurocognitive impairment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.numecd.2021.03.026DOI Listing
July 2021

Metabolic Defects of Peripheral T Cells in COVID-19 Patients.

J Immunol 2021 06 28;206(12):2900-2908. Epub 2021 May 28.

Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital; The Second Affiliated Hospital, School of Medicine, South University of Science and Technology, Shenzhen, Guangdong Province, China;

The relatively low partial pressure of oxygen, reduced oxygen saturation, and aberrant plasma metabolites in COVID-19 may alter energy metabolism in peripheral immune cells. However, little is known regarding the immunometabolic defects of T cells in COVID-19 patients, which may contribute to the deregulated immune functions of these cells. In this study, we longitudinally characterized the metabolic profiles of resting and activated T cells from acutely infected and convalescent COVID-19 patients by flow cytometry and confirmed the metabolic profiles with a Seahorse analyzer. Non-COVID-19 and healthy subjects were enrolled as controls. We found that ex vivo T cells from acutely infected COVID-19 patients were highly activated and apoptotic and displayed more extensive mitochondrial metabolic dysfunction, especially cells in CD8 T cell lineages, than those from convalescent COVID-19 patients or healthy controls, but slightly disturbed mitochondrial metabolic activity was observed in non-COVID-19 patients. Importantly, plasma IL-6 and C-reactive protein (CRP) levels positively correlated with mitochondrial mass and negatively correlated with fatty acid uptake in T cells from COVID-19 patients. Additionally, compared with those from healthy controls, in vitro-activated T cells from acutely infected COVID-19 patients showed signs of lower glycolysis, a reduced glycolytic capacity, and a decreased glycolytic reserve, accompanied by lower activation of mTOR signaling. Thus, newly identified defects in T cell mitochondrial metabolic functions and metabolic reprogramming upon activation might contribute to immune deficiency in COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4049/jimmunol.2100068DOI Listing
June 2021

Elevated fibroblast growth factor-inducible 14 expression transforms proneural-like gliomas into more aggressive and lethal brain cancer.

Glia 2021 Sep 15;69(9):2199-2214. Epub 2021 May 15.

Department of Neurosurgery, University of Maryland School of Medicine, Baltimore, Maryland, USA.

High-grade gliomas (HGGs) are aggressive, treatment-resistant, and often fatal human brain cancers. The TNF-like weak inducer of apoptosis (TWEAK)/fibroblast growth factor-inducible 14 (Fn14) signaling axis is involved in tissue repair after injury and constitutive signaling has been implicated in the pathogenesis of numerous solid cancers. The Fn14 gene is expressed at low levels in the normal, uninjured brain but is highly expressed in primary isocitrate dehydrogenase wild-type and recurrent HGGs. Fn14 signaling is implicated in numerous aspects of glioma biology including brain invasion and chemotherapy resistance, but whether Fn14 overexpression can directly promote tumor malignancy has not been reported. Here, we used the replication-competent avian sarcoma-leukosis virus/tumor virus A system to examine the impact of Fn14 expression on glioma development and pathobiology. We found that the sole addition of Fn14 to an established oncogenic cocktail previously shown to generate proneural-like gliomas led to the development of highly invasive and lethal brain cancer with striking biological features including extensive pseudopalisading necrosis, constitutive canonical and noncanonical NF-κB pathway signaling, and high plasminogen activator inhibitor-1 (PAI-1) expression. Analyses of HGG patient datasets revealed that high human PAI-1 gene (SERPINE1) expression correlates with shorter patient survival, and that the SERPINE1 and Fn14 (TNFRSF12A) genes are frequently co-expressed in bulk tumor tissues, in tumor subregions, and in malignant cells residing in the tumor microenvironment. These findings provide new insights into the potential importance of Fn14 in human HGG pathobiology and designate both the NF-κB signaling node and PAI-1 as potential targets for therapeutic intervention. MAIN POINTS: This work demonstrates that elevated levels of the TWEAK receptor Fn14 in tumor-initiating, neural progenitor cells leads to the transformation of proneural-like gliomas into more aggressive and lethal tumors that exhibit constitutive NF-κB pathway activation and plasminogen activator inhibitor-1 overexpression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/glia.24018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596752PMC
September 2021

Role of Neutrophil-Derived S100B in Acute Myocardial Infarction Patients From the Han Chinese Population.

Front Cardiovasc Med 2020 16;7:595446. Epub 2021 Mar 16.

Cardiovascular Research Institute and Department of Cardiology, The General Hospital of Northern Theater Command, Shenyang, China.

This study aimed to clarify the novel role of homeostatic calmodulin S100B and determined whether S100B genetic variants affected atherosclerosis progression in acute myocardial infarction (AMI) patients. Plasma levels of S100B were measured systemically in AMI patients, stable angina pectoris patients, and control subjects. S100B was obtained from the human coronary artery thrombi using a thrombectomy catheter and quantified via immunohistochemical analysis, qRT-PCR and Western blot analyse. We also screened for S100B variations (rs9722, rs9984765, rs2839356, rs1051169, and rs2186358) via direct sequencing, and investigated the relationship between these variants and AMI patients in the Chinese Han population. Plasma S100B levels increased significantly in AMI patients compared to the levels in stable angina pectoris patients and control subjects (119.45 ± 62.46, 161.96 ± 73.30, and 312.91 ± 127.59 pg/ml, respectively). Immunohistochemical staining results showed that S100B expression was increased in the neutrophils of coronary artery thrombi obtained from AMI patients, as compared to that in normal blood clot, and S100B expression was significantly increased in fresh thrombi tissues, as compared to that in organized thrombi tissues. Western blot and qRT-PCR analysis showed that S100B expression increased in coronary artery thrombi, as compared to that in normal blood clots. After pre-treating the neutrophils with siRAGE, the neutrophils migration induced by S100B were abolished through the NFκB-IL1β/IL6 signaling pathway. Compared to their corresponding wild-type genotypes, the S100B rs9722 variant was associated with increased susceptibility to AMI (OR = 1.35, 95%CI: 1.12-1.65, = 0.02). Individuals with the S100B 9722 A allele had higher plasma S100B levels than those with the G allele in control subjects and AMI patients (141.70 ± 76.69 vs. 107.31 ± 56.05 and 347.13 ± 148.94 vs. 273.05 ± 133.62, respectively). Levels of neutrophil-derived S100B, a novel homeostatic calmodulin, were elevated in the early stages of myocardial infarction. The S100B rs9722 allele was independently associated with AMI patients in the Han Chinese population.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2020.595446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008063PMC
March 2021

Brain ethanol metabolism by astrocytic ALDH2 drives the behavioural effects of ethanol intoxication.

Nat Metab 2021 03 22;3(3):337-351. Epub 2021 Mar 22.

Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD, USA.

Alcohol is among the most widely used psychoactive substances worldwide. Ethanol metabolites such as acetate, thought to be primarily the result of ethanol breakdown by hepatic aldehyde dehydrogenase 2 (ALDH2), contribute to alcohol's behavioural effects and alcoholism. Here, we show that ALDH2 is expressed in astrocytes in the mouse cerebellum and that ethanol metabolism by astrocytic ALDH2 mediates behavioural effects associated with ethanol intoxication. We show that ALDH2 is expressed in astrocytes in specific brain regions and that astrocytic, but not hepatocytic, ALDH2 is required to produce ethanol-derived acetate in the mouse cerebellum. Cerebellar astrocytic ALDH2 mediates low-dose ethanol-induced elevation of GABA levels, enhancement of tonic inhibition and impairment of balance and coordination skills. Thus, astrocytic ALDH2 controls the production, cellular and behavioural effects of alcohol metabolites in a brain-region-specific manner. Our data indicate that astrocytic ALDH2 is an important, but previously under-recognized, target in the brain to alter alcohol pharmacokinetics and potentially treat alcohol use disorder.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s42255-021-00357-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294184PMC
March 2021

Spatial patterns of net greenhouse gas balance and intensity in Chinese orchard system.

Sci Total Environ 2021 Jul 6;779:146250. Epub 2021 Mar 6.

Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Xiamen Key Lab of Urban Metabolism, Xiamen 361021, China. Electronic address:

Fruit production has been expanding due to the pursuit of healthier lifestyles in China. Determining the greenhouse gas (GHG) emissions status of the orchard system could contribute to adopting appropriate measures to alleviate climate change pressure from the growing fruit production. In this study, the net GHG balance and GHG intensity (GHGI) in the Chinese fruit production were estimated at the regional level using a meta-analysis based on databases compiled from relevant publications during 2000-2019, including soil nitrous oxide (NO) and methane (CH) emissions or uptake, upstream carbon dioxide (CO) emissions related to farm practices, and the change of soil organic carbon (SOC) storage from the life cycle perspective. Results showed that the net GHG balance and GHGI varied among six regions, with ranges of 6.4 ± 0.3 to 10.0 ± 0.6 Mg COe ha yr, and 2.2 ± 0.2 to 3.0 ± 0.2 kg COe kg, respectively. Synthetic nitrogen (N) fertilization was the largest source of overall GHG emissions from fruit production throughout China, accounting for 46% and ranging from 43% to 55% in the six fruit production regions. Fertilizer-induced NO emissions were responsible for 22-31% of the total GHG emissions, and the NO-N emission factor was identified as 0.7%. Also, power use for irrigation contributed a non-negligible 17% to the emissions on a national level, yet with large regional variations. In addition, fruit production in North, Northeast, Central, and East, and South China have relatively lower GHGIs than in Northwest and Southwest China. The estimated total GHG emissions from the Chinese fruit production were 102 Tg COe, with the contribution of SOC change to a decrease by 11% for the year 2018. Our results highlight an urgency to lower fruit production-related carbon emissions by extending optimized N fertilization and irrigation modes in China's orchard system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.146250DOI Listing
July 2021

Type A aortic dissection developed after type B dissection with the presentation of shoulder pain: A case report.

World J Clin Cases 2021 Jan;9(1):232-235

Faculty of Nursing, School of Medicine, Hunan Normal University, Changsha 410013, Hunan Province, China.

Background: Aortic dissection (AD) is a life-threatening condition with a high mortality rate without immediate medical attention. Early diagnosis and appropriate treatment are critical in treating patients with AD. In the emergency department, patients with AD commonly present with classic symptoms of unanticipated severe chest or back pain. However, it is worth noting that atypical symptoms of AD are easily misdiagnosed.

Case Summary: A 51-year-old woman was first diagnosed with scapulohumeral periarthritis due to left shoulder pain. After careful examination of her previous medical history and contrast-enhanced computed tomography angiography, the patient was diagnosed with a new type A AD after chronic type B dissection in the ascending aorta. The patient was successfully treated with surgical replacement of the dissected aortic arch and remains in good health.

Conclusion: New retrograde type A AD after chronic type B dissection is relatively rare. It is worth noting that a physician who has a patient with suspected AD should be vigilant. Both patient medical history and imaging tests are crucial for a more precise diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12998/wjcc.v9.i1.232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809677PMC
January 2021

Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells.

Sci Rep 2021 01 28;11(1):2504. Epub 2021 Jan 28.

School of Public Health, Bengbu Medical College, Bengbu, 233030, People's Republic of China.

Sulforaphane (SFN) extracted from broccoli sprout has previously been investigated for its potential properties in cancers, however, the underlying mechanisms of the anticancer activity of SFN remain not fully understood. In the present study, we investigate the effects of SFN on cell proliferation, cell cycle, cell apoptosis, and also the expression of several cell cycle and apoptosis-related genes by MTT assay, flow cytometry and western blot analysis in gastric cancer (GC) cells. The results showed that SFN could impair the colony-forming ability in BGC-823 and MGC-803 cell lines compared with the control. In addition, SFN significantly suppressed cell proliferation by arresting the cell cycle at the S phase and enhancing cell apoptosis in GC cells in a dose-dependent manner. Western blot results showed that SFN treatment significantly increased the expression levels of p53, p21 and decreased CDK2 expression, which directly regulated the S phase transition. The Bax and cleaved-caspase-3 genes involved in apoptosis executive functions were significantly increased in a dose-dependent manner in BGC-823 and MGC-803 cells. These results suggested that SFN-induced S phase cell cycle arrest and apoptosis through p53-dependent manner in GC cells, which suggested that SFN has a potential therapeutic application in the treatment and prevention of GC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-81815-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843980PMC
January 2021

Leucine heptad motifs within transmembrane domains affect function and oligomerization of human organic anion transporting polypeptide 1B1.

Biochim Biophys Acta Biomembr 2021 04 8;1863(4):183554. Epub 2021 Jan 8.

College of Life Sciences, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, South China Agricultural University, Guangzhou, China. Electronic address:

Organic anion transporting polypeptides (OATPs) are transmembrane proteins responsible for the uptake of a wide range of endogenous compounds and clinically important drugs. The liver-specific OATP1B1 serves crucial roles in the removal of many orally administered drugs. The proper function of the transporter hence is essential for the pharmacokinetics of various therapeutic agents. Membrane proteins tend to form oligomers that are important for their stability, targeting and/or interactions with the substrates. Previous study in our laboratory revealed that OATP1B1 may form homo-oligomers and that a GXXXG motif localized at transmembrane domain 8 (TM8) may affect its oligomerization. In the current study, three short-form leucine heptad repeats within the transmembrane domains of OATP1B1 were investigated. It was found that the disruption of leucine heptad repeats within TM3 dramatically reduced the uptake function and protein-protein association of OATP1B1; while within TM8, only L378 is essential for the function of OATP1B1 and alanine replacement of L378 exhibited no effect on the oligomerization. The fragmental expression of TM3 interfered with the association of OATP1B1 homo-oligomers as well as its association with OATP1B3, which is also selectively expressed at human hepatocytes, suggesting that the region may be shared by both transporters for their protein-protein interactions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamem.2021.183554DOI Listing
April 2021

Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.

Int J Biol Macromol 2021 Feb 6;171:177-184. Epub 2021 Jan 6.

Food and Pharmaceutical Engineering Institute, Guiyang University, Guiyang, Guizhou 550005, China.

A water-soluble polysaccharide (LCP-05) was isolated from the flowers of Leucosceptrum canum Smith. LCP-05 was an acidic polysaccharide with a molecular weight of approximately 8.9 kDa. Monosaccharide composition analysis indicated that LCP-05 was composed of Man, Rha, GlcA, GalA, Glc, Gal and Ara in a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22. The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues. The side chains are terminated primarily with the Araf and Glcp residues. LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.01.009DOI Listing
February 2021

Design of a bi-functional NaScF: Yb/Er nanoparticles for deep-tissue bioimaging and optical thermometry through Mn doping.

Talanta 2021 Mar 30;224:121832. Epub 2020 Oct 30.

State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 3888 Eastern South Lake Road, Changchun, 130033, China. Electronic address:

An approximately monochromatic red upconversion (UC) emission is successfully realized in NaScF: Yb/Er nanoparticles (NPs) through Mn ions doping without phase transition. The Mn ions play a role of bridge during the energy transfer process from green emission state H/S of Er to red emission state F of Er, which significantly accelerates the red UC enhancement. The strongest red luminescence is observed in the sample containing 10% Mn ions (Mn-10) with an enhancement factor of 7.5 times. Meanwhile, an ultrasensitive optical thermometry in the physiological temperature region can be realized by utilizing the fluorescence intensity ratio (FIR) between two thermally coupled Stark transitions of Er: I → I, locating in the near-infrared (NIR) long wavelength region of the second biological window. Its relative sensitivity S can be expressed by 340/T, which is much higher than most optical thermometers based on thermally coupled Stark sublevels reported by the previous papers. Beyond that, an ex vivo experiment is designed to evaluate the penetration depth of the red and NIR emission of Mn-10 in the biological tissues, revealing that they can reach depth of at least 3 mm and 5 mm respectively. More importantly, the increasing tissue thickness has almost no effect on the FIR values. All the results show that the present sample is a promising bi-functional nano probe which can be used for bioimaging and temperature sensing in the deep tissues through the strong red UC emission and ultrasensitive NIR optical thermometer, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2020.121832DOI Listing
March 2021

MiR-4497 mediates oxidative stress and inflammatory injury in keratinocytes induced by ultraviolet B radiation through regulating NF-κB expression.

G Ital Dermatol Venereol 2020 Dec 14. Epub 2020 Dec 14.

Department of Dermatology.

Background: To investigate the role and underlying mechanism of miR-4497 in oxidative stress and inflammatory injury in keratinocytes induced by ultraviolet B (UVB) radiation.

Methods: An injury model of keratinocytes induced by UVB radiation was constructed. RT-qPCR, MTT assay and flow cytometry were adopted to detect miR-4497 expression in HaCaT cells, cell proliferation, and cell apoptosis, respectively. The levels of cytokines TNF-α, IL-18, IL-6 and IL-1β in cell culture supernatant were tested by ELISA. ROS levels in the cells were labeled by DCFH-DA fluorescent probe, and then quantitative fluorescence analysis was performed by flow cytometry. SOD activity in the cells was measured by Xanthine Oxidase assay kit. Western blot was used to determine NF-κB expression in cytoplasm and nucleus, and p-IκBα expression in the cells.

Results: UVB radiation significantly increased miR-4497 expression in HaCaT cells, inhibited cell proliferation, and promoted cell apoptosis. Meanwhile, UVB radiation caused the promotion of secretion of cytokines TNF-α, IL-18, IL-6 and IL-1β. The production of reactive oxygen species (ROS) was promoted by UVB radiation, while SOD activity was inhibited. Nuclear transfer of NF-κB signal was also induced by UVB radiation. In addition, downregulation of miR-4497 expression significantly inhibited the effects of UVB radiation on cell proliferation, apoptosis, cytokine secretion, redox level and NF-κB signal in HaCaT cells, while overexpression of miR-4497 further enhanced these effects of UVB radiation on HaCaT cells.

Conclusions: UVB may promote the expression of inflammatory and oxidative stress signals in keratinocytes by upregulating miR-4497 expression, thus mediating cell injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.23736/S0392-0488.20.06825-XDOI Listing
December 2020
-->