Publications by authors named "Xu Meng"

1,158 Publications

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Only zinc (Zn) among micronutrients is an important predictor of grassland production.

Front Plant Sci 2022 22;13:962513. Epub 2022 Jul 22.

Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, China.

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http://dx.doi.org/10.3389/fpls.2022.962513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355639PMC
July 2022

A novel cuproptosis-related prognostic lncRNA signature and lncRNA MIR31HG/miR-193a-3p/TNFRSF21 regulatory axis in lung adenocarcinoma.

Front Oncol 2022 22;12:927706. Epub 2022 Jul 22.

Department of Oncology, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China.

Lung adenocarcinoma (LUAD) remains the most common subtype of lung malignancy. Cuproptosis is a newly identified cell death which could regulate tumor cell proliferation and progression. Long non-coding RNAs (lncRNAs) are key molecules and potential biomarkers for diagnosing and treating various diseases. However, the effects of cuproptosis-related lncRNAs on LUAD are still unclear. In our study, 7 cuproptosis-related lncRNAs were selected to establish a prognostic model using univariate Cox regression analysis, LASSO algorithm, and multivariate analysis. Furthermore, we evaluated AC008764.2, AL022323.1, ELN-AS1, and LINC00578, which were identified as protective lncRNAs, while AL031667.3, AL606489.1, and MIR31HG were identified as risk lncRNAs. The risk score calculated by the prognostic model proved to be an effective independent factor compared with other clinical features by Cox regression analyses [univariate analysis: hazard ratio (HR) = 1.065, 95% confidence interval (CI) = 1.043-1.087, < 0.001; multivariate analysis: HR = 1.067, 95% CI = 1.044-1.091, < 0.001]. In addition, both analyses (ROC and nomogram) were used to corroborate the accuracy and reliability of this signature. The correlation between cuproptosis-related lncRNAs and immune microenvironment was elucidated, where 7 immune cells and 8 immune-correlated pathways were found to be differentially expressed between two risk groups. Furthermore, our results also identified and verified the ceRNA of cuproptosis-related lncRNA MIR31HG/miR-193a-3p/TNFRSF21 regulatory axis using bioinformatics tools. MIR31HG was highly expressed in LUAD specimens and some LUAD cell lines. Inhibition of MIR31HG clearly reduced the proliferation, migration, and invasion of the LUAD cells. MIR31HG showed oncogenic features sponging miR-193a-3p and tended to positively regulate TNFRSF21 expression. In a word, lncRNA MIR31HG acts as an oncogene in LUAD by targeting miR-193a-3p to modulate TNFRSF21, which may be beneficial to the gene therapy of LUAD.
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http://dx.doi.org/10.3389/fonc.2022.927706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353736PMC
July 2022

dGLYAT modulates Gadd45-mediated JNK activation and cell invasion.

Cell Div 2022 Aug 6;17(1). Epub 2022 Aug 6.

The First Rehabilitation Hospital of Shanghai, Shanghai Key Laboratory of Signaling and Diseases Research, School of Life Science and Technology, Tongji University, Shanghai, China.

Background: Cell invasion is a crucial step of tumor metastasis, finding new regulators of which offers potential drug targets for cancer therapy. Aberrant GLYAT expression is associated with human cancers, yet its role in cancer remains unknown. This study aims to understand the function and mechanism of Drosophila GLYAT in cell invasion.

Results: We found that dGLYAT regulates Gadd45-mediated JNK pathway activation and cell invasion. Firstly, loss of dGLYAT suppressed scrib depletion- or Egr overexpression-induced JNK pathway activation and invasive cell migration. Secondary, mRNA-seq analysis identified Gadd45 as a potential transcriptional target of dGLYAT, as depletion of dGLYAT decreased Gadd45 mRNA level. Finally, Gadd45 knockdown suppressed scrib depletion-induced JNK pathway activation and cell invasion.

Conclusions: These evidences reveal the role of dGLYAT and Gadd45 in JNK-dependent cell invasion, and provide insight for the roles of their human homologs in cancers.
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http://dx.doi.org/10.1186/s13008-022-00080-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357319PMC
August 2022

Complications and oncological outcomes after salvage surgery for recurrent and residual hypopharyngeal squamous cell carcinoma: a retrospective cohort study.

Ann Transl Med 2022 May;10(9):525

Department of Head and Neck Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Salvage surgery for hypopharyngeal squamous cell carcinoma (HPSCC) after radiotherapy may result in several postoperative complications and the oncological outcome is unsatisfying. Therefore, identifying the risk factors for postoperative complications and oncological outcome after salvage surgery is important. This study aimed to determine which HPSCC patients might benefit from salvage surgery following previous radiotherapy.

Methods: We retrospectively analyzed 91 HPSCC patients who underwent salvage surgery due to locoregional recurrence/residual disease after radiotherapy. The pre- and intraoperative characteristics with complications and oncological outcomes were collected through medical records and telephone follow-up. Risk factors for complications were analyzed by binary logistic regression. The oncological outcomes were assessed by overall survival (OS) after salvage surgery. Kaplan-Meier curves and Cox proportional hazard regression analysis were used for univariate and multivariate survival analyses.

Results: Postoperative complications occurred in 40.7% of patients, with pharyngo-cutaneous fistula (PCF) occurring in 29.7% of patients. Salvage surgery for local disease was the only independent risk factor for postoperative complications and PCF [complications: odds ratio (OR) =5.298, 95% confidence interval (CI): 1.163-24.130, P=0.031; PCF: OR =4.543, 95% CI: 1.187-17.387, P=0.027). In the subgroup of patients with local disease, time of curative treatment initiation >90 days (OR =7.331, 95% CI: 1.278-42.054, P=0.025) and preoperative hemoglobin <118 g/L (OR =10.101, 95% CI: 1.026-99.492, P=0.045) were independent risk factors for postoperative complications, while free flap reconstruction was an independent protective factor for PCF (OR =0.099, 95% CI: 0.010-0.934, P=0.043). The median OS time was 17 months, with 5-year OS rates of 30%. Age at salvage surgery <50 years [hazard ratio (HR) =2.047, 95% CI: 1.217-3.443, P=0.007] and recurrence or retreatment clinical T stage 3-4 (rcT3-4) (HR =2.051, 95% CI: 1.219-3.450, P=0.007) were identified as risk factors for OS. The 5-year OS rates of patients without and with both risk factors were 43% and 10% (P=0.001).

Conclusions: Salvage surgery for locoregional recurrence/residual disease after previous radiotherapy could improve survival in selected patients with HPSCC. Patients with local recurrence/residual disease had a higher complication rate. Efforts can be made to shorten the time of curative treatment initiation and treat anemia to reduce the risk of postoperative complications in this subgroup.
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http://dx.doi.org/10.21037/atm-22-1844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347053PMC
May 2022

Evolutionary trends in human mandibles and dentition from Neolithic to current Chinese.

Arch Oral Biol 2022 Jul 20;142:105512. Epub 2022 Jul 20.

School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Shandong, PR China. Electronic address:

Objectives: This study aimed to systematically compare Neolithic mandibles and dentition with modern Chinese, and thereby discern human evolutionary trends.

Materials And Methods: Neolithic remains of 45 adults unearthed at the Zhangqiu Jiaojia site, were compared with clinical records of 48 patients at Shandong University. All samples were scanned by cone beam computed tomography (CBCT) using identical parameters. Digital imaging and communications in medicine images were collected, three-dimensional models reconstructed, and morphology measurements obtained using Mimics software.

Results: Neolithic mandibles were significantly larger in their vertical and sagittal dimensions (P < .05), but similar in horizontal width to modern humans. Their condyles had fewer bird beak and crooked finger shaped morphologies than modern mandibles (P < .05). Neolithic third molars were more often erupted than in modern mandibles, and their Position A, class I and II, and vertical impactions were more common (P < .05). Neolithic teeth were generally smaller in crown lengths and in cross-sectional areas, than their modern counterparts (P < .05).

Conclusions: Neolithic mandibles were larger than modern humans, who have refined diets and mandibular atrophy. They had fewer abnormally shaped condylar morphologies, and much fewer third molar impactions than in modern humans. However, modern dentition particularly their crowns are larger, likely through genetic influx from migrations.
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http://dx.doi.org/10.1016/j.archoralbio.2022.105512DOI Listing
July 2022

Mobile Terminal Equipment and Methods of Martial Arts Movement Correction in Intelligent Physical Education Environment.

Authors:
Meng Xu Yaya Shi

Comput Intell Neurosci 2022 21;2022:7390836. Epub 2022 Jul 21.

Department of Physical Education, Gangneung-Wonju National University, Gangneung 25457, Gangwon-do, Republic of Korea.

In recent years, the physical quality of middle school students in China has generally declined, which has attracted the attention of the state and the Ministry of education. With the development of Internet technology, China's physical education teaching environment has gradually become intelligent. This article mainly studies the mobile terminal equipment and methods of martial arts movement correction in the intelligent physical education environment. 21 young martial arts athletes were selected as the research objects. In the experiment, functional screening (FMS) was used to test the martial arts athletes, followed by FMS tests and scores. A video camera was used to record the motion test from the subjects' sagittal and frontal planes. Using wireless sensor technology to collect the athletes' motion signals, after the twelfth week, the FMS, SEBT, and the number of successful routines were tested on the two groups of athletes, respectively. The pre-test data and the post-correction data of the two test indicators were compared and analyzed. There is no significant difference. The necessary statistics and integration of the obtained data are carried out by using the calculation methods in sports statistics. The experimental data showed that the average total scores of FMS screening test of Changquan athletes and Taijiquan athletes were 14.71 ± 1.52 and 16.20 ± 1.32, respectively. The results of the research on the mobile terminal equipment and methods of martial arts movement correction in the intelligent sports environment show that the mobile terminal equipment can improve the independent training ability of athletes, and at the same time has a good correction effect on irregular movements.
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http://dx.doi.org/10.1155/2022/7390836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334115PMC
August 2022

Exotic fishes that are phylogenetically close but functionally distant to native fishes are more likely to establish.

Glob Chang Biol 2022 Jul 29. Epub 2022 Jul 29.

Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Prevention and Control for Aquatic Invasive Alien Species, Ministry of Agriculture and Rural Affairs; Key Laboratory of Alien Species and Ecological Security (CAFS), Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.

Since Darwin's time, degree of ecological similarity between exotic and native species has been assumed to affect the establishment success or failure of exotic species. However, a direct test of the effect of exotic-native similarity on establishment of exotics is scarce because of the difficulty in recognizing failures of species to establish in the field. Here, using a database on the establishment success and failure of exotic fish species introduced into 673 freshwater lakes, we evaluate the effect of similarity on the establishment of exotic fishes by combining phylogenetic and functional information. We illustrate that, relative to other biotic and abiotic factors, exotic-native phylogenetic and functional similarities were the most important correlates of exotic fish establishment. While phylogenetic similarity between exotic and resident fish species promoted successful establishment, functional similarity led to failure of exotics to become established. Those exotic species phylogenetically close to, but functionally distant from, native fishes were most likely to establish successfully. Our findings provide a perspective to reconcile Darwin's naturalization conundrum and suggest that, while phylogenetic relatedness allows exotic fish species to pre-adapt better to novel environments, they need to possess distinct functional traits to reduce competition with resident native fish species.
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http://dx.doi.org/10.1111/gcb.16360DOI Listing
July 2022

Age-related twin-peak prevalence profiles of H. pylori infection, gastritis, GIN and gastric cancer: Analyses of 70,534 patients with gastroscopic biopsies.

PLoS One 2022 21;17(7):e0265885. Epub 2022 Jul 21.

Department of Pathology, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China.

Objectives: H. pylori (Hp) infection has been indicated in the pathogenesis of gastric diseases including gastric cancer (GC). This study aimed at exploring the relationships between Hp infection and gastric diseases including GC in a large dataset of routine patients undergoing gastroscopy.

Methods: From November 2007 to December 2017, 70,534 first-time visiting patients aged 18-94 years with gastroscopic biopsies were histologically diagnosed and analyzed. Patients' data were entered twice in an Excel spreadsheet database and analyzed using the SPSS (version 22.0) software package and statistical significance was defined as P<0.05 for all analyses.

Results: The first interesting observation was age-related twin-peak prevalence profiles (TPPs) for Hp infection, gastritis, and advanced diseases with different time spans (TS) between the first and second occurring peaks. Hp infection and gastritis had TPPs occurring at earlier ages than TPPs of gastric introepithelial neoplasia (GIN) and GC. More patients were clustered at the second occurring TPPs. The time spans (TS) from the first occurring peak of Hp infection to the first occurring peaks of other gastric diseases varied dramatically with 0-5 years for gastritis; 5-15 years for GINs, and 5-20 years for GC, respectively. The number of males with Hp infection and gastric diseases, excluding non-atrophic gastritis (NAG), was more than that of females (P<0.001).

Conclusions: We have first observed age-related twin-peak prevalence profiles for Hp infection, gastritis, GIN, and GC, respectively, among a large population of patients undergoing gastroscopy. The second prevalence peak of GC is at ages of 70-74 years indicating that many GC patients would be missed during screening because the cut-off age for screening is 69 years old in China.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0265885PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302749PMC
July 2022

[Chemical constituents with α-glucosidase inhibitory activities from seeds of Morinda citrifolia (Noni)].

Zhongguo Zhong Yao Za Zhi 2022 Jul;47(13):3519-3525

School of Pharmacy, Fujian University of Traditional Chinese Medicine Fuzhou 350122, China Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education, Hainan Normal University Haikou 571158, China Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province, College of Chemistry and Chemical Engineering,Hainan Normal University Haikou 571158, China.

The present study investigated the chemical constituents and inhibitory activities against α-glucosidase from the seeds of Morinda citrifolia(Noni) by the chromatographic technique and semi-preparative HPLC.Fifteen compounds were isolated from the ethyl acetate extract of the seeds, and their structures were identified on the basis of physiochemical characteristics and spectroscopic data as(9S,2E,4Z,7Z)-9-hydroxydeca-2,4,7-trienoic acid(1), azelaic acid(2), scopoletin(3), ursolic acid(4), quercetin(5), cyclo-(L-Leu-L-Ile)(6), cyclo-(L-Phe-L-Ile)(7), cyclo-(L-Phe-L-Val)(8), cyclo-(L-Leu-L-Val)(9), cyclo-(L-Phe-L-Leu)(10), caffeic acid(11), 3,4-dihydroxycinnamaldehyde(12), p-hydroxybenzoic acid(13), p-hydroxy-cinnamic acid(14), and p-hydroxyphenethyl alcohol(15).Among them, compound 1 was a new fatty acid and compounds 7-10 and 12 were isolated from Morinda plant in the Rubiaceae family for the first time.Compounds 1, 2 and 4-15 were isolated from the seeds of M.citrifolia(Noni) for the first time.All isolated compounds were evaluated for the inhibitory activities against α-glucosidase and compounds 3-5 showed potential inhibitory activity with IC_(50) values of 160, 133, and 120 μmol·L~(-1), respectively.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20211228.201DOI Listing
July 2022

DDX3 acts as a tumor suppressor in colorectal cancer as loss of DDX3 in advanced cancer promotes tumor progression by activating the MAPK pathway.

Int J Biol Sci 2022 6;18(10):3918-3933. Epub 2022 Jun 6.

Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.

The treatment and prognosis of patients with advanced colorectal cancer (CRC) remain a difficult problem. Herein, we investigated the role of DEAD (Asp-Glu-Ala-Asp) box helicase 3 (DDX3) in CRC and proposed potential therapeutic targets for advanced CRC. The expression of DDX3 in CRC and its effect on prognosis were explored by databases and CRC tissue microarrays. Stable DDX3 knockdown and overexpression cell lines were established with lentiviral vectors. The effects of DDX3 on CRC were investigated by functional experiments and . The molecular mechanism of DDX3 in CRC was explored by western blotting. Molecular-specific inhibitors were further used to explore potential therapeutic targets for advanced CRC. The expression of DDX3 was decreased in advanced CRC, and patients with low DDX3 expression had a poor prognosis. and experiments showed that low DDX3 expression promoted the proliferation, migration and invasion of CRC. DDX3 loss regulated E-cadherin and β-catenin signaling through the mitogen-activated protein kinase (MAPK) pathway as shown by western blotting. In addition, the MEK inhibitor, PD98059, significantly reduced the increased cell proliferation, migration and invasion caused by knockdown of DDX3. DDX3 acts as a tumor suppressor gene in CRC. DDX3 loss in advanced cancer promotes cancer progression by regulating E-cadherin and β-catenin signaling through the MAPK pathway, and targeting the MAPK pathway may be a therapeutic approach for advanced CRC.
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http://dx.doi.org/10.7150/ijbs.73491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274493PMC
July 2022

LOXL3-promoted hepatocellular carcinoma progression via promotion of Snail1/USP4-mediated epithelial-mesenchymal transition.

Environ Toxicol 2022 Jul 16. Epub 2022 Jul 16.

Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Lysyl-oxidase-like 3 (LOXL3) was reported to be essential in epithelial-mesenchymal transition (EMT) of cancers. However, the role of LOXL3 in hepatocellular carcinoma (HCC) remained unclear. In this study, we explored clinical significance, biological functions, and regulatory mechanisms of LOXL3 in HCC. Our study found that LOXL3 expression was markedly associated with the tumor size and clinical stage of HCC, and it was highly expressed in tumor tissues of metastatic HCC patients. High expression of LOXL3 predicted a poor prognosis of HCC. TGF-β1 treatment elevated LOXL3 protein expression and cell invasion, and reduced cell apoptosis in HCC cell lines (SMMC-7721 and Huh-7), while downregulation of LOXL3 reversed the promotive effects of TGF-β1 treatment on LOXL3 protein expression and cell invasion, and the inhibitory effect on cell apoptosis. Mechanistically, LOXL3 interacted with snail family transcriptional repressor 1 (Snail1) through STRING database and RIP assay, and Snail1 bound to ubiquitin-specific peptidase 4 (USP4) promoter by JASPAR database, luciferase reporter gene and Co-IP assays. Overexpression of USP4 reversed the inhibitory effect of LOXL3 silence on EMT in HCC cells through deubiquitinating and stabilizing the expression of Snail1. Moreover, LOXL3-promoted HCC EMT through Wnt/β-catenin/Snail1 signaling pathway. In vivo study revealed that silence of LOXL3-inhibited HCC tumor growth. In conclusion, LOXL3 silence inhibited HCC invasion and EMT through Snail1/USP4-mediated circulation loop and Wnt/β-catenin signaling pathway.
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http://dx.doi.org/10.1002/tox.23617DOI Listing
July 2022

Contralateral patent processus vaginalis repair in boys: a single-center retrospective study.

Sci Rep 2022 Jul 15;12(1):12073. Epub 2022 Jul 15.

Bachelor of Science in Medicine, Linyi Central Hospital, Linyi, Shandong, China.

To ascertain the prevalence of contralateral patent processus vaginalis (CPPV) in life and the significance of the prevalence trends for treatment. We performed a retrospective review of all inguinal hernias (IHs) that underwent repair in our hospital from 2014 to 2018. We analyzed the frequency of occurrence and treatment in boys. We assessed and compared the history, initial sides of hernia, CPPV and prognoses in different age groups. We assessed all IH cases repaired in our hospital and selected male patients of a variety of ages, including boys and men. Recurrent cases were not enrolled. A total of 3243 cases were enrolled: 2489 [right-sided IH 1411 (56.69%) vs. left-sided IH 975 (39.17%), bilateral IH 103 (4.14%)] in children and 754 [right-sided IH 485 (64.32%) vs. left-sided IH 236 (31.30%), bilateral IH 33 (4.38%)] in adults. A total of 1124 CPPVs were identified in children with unilateral IH (2386), and 12 were identified in adults (267) (p < 0.0001). There were no significant differences in recurrence rate between different subgroups of children (p > 0.05). The incidence of IH in boys was significantly higher than that in men. The number of incident cases declines rapidly with age in boys. The processus vaginalis is normally obliterated and involuted but may instead remain patent for a long period before closure; routine exploration on the contralateral side may eliminate the possibility of spontaneous PPV closure.
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http://dx.doi.org/10.1038/s41598-022-15435-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287543PMC
July 2022

The effect of afatinib in a pretreated patient with invasive mucinous adenocarcinoma of the lung harboring YVMA insertion: a case report.

Transl Cancer Res 2022 Jun;11(6):1819-1823

Department of Respiratory Medicine, First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou, China.

Background: Invasive mucinous adenocarcinoma (IMA) is an uncommon variant of lung adenocarcinoma. The survival data and therapeutic methods for IMAs are limited. The frequency of human epidermal growth factor receptor 2 () mutations in IMAs is low, and the clinical benefit of HER2 inhibitors in patients with IMA is still being explored. Afatinib is a pan-HER inhibitor and the studies of afatinib treatment in IMA patients are very limited.

Case Description: Herein, we present the case of a 49-year-old female, never-smoker stage IVa IMA patient with persistent cough and sputum expectoration diagnosed with stage IVa IMA. Polymerase chain reaction (PCR) and next-generation sequencing (NGS) were utilized to detect mutations for targeted therapies on lung biopsy, but no mutation was found. After treatment failures of chemotherapy and a multiple-kinase inhibitor, liquid biopsy identified exon 20 insertion p.A775_G776insYVMA with NGS. The patient was then treated with afatinib as the third-line treatment. Following administration for one month, the patient's symptoms of coughing, sputum expectoration, and dyspnea improved. Stable disease (SD) was observed, and the patient achieved durable clinical benefit with prolonged progression-free survival (PFS) of 20 months. Her overall survival was 5.8 years.

Conclusions: This is the first report of afatinib treatment achieving long-lasting and stable disease control in an IMA patient with a exon 20 YVMA insertion. Our results will help to improve the treatment of IMA.
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http://dx.doi.org/10.21037/tcr-22-1457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273679PMC
June 2022

Crystal Structure Based Mutagenesis of Cattleyene Synthase Leads to the Generation of Rearranged Polycyclic Diterpenes.

Angew Chem Int Ed Engl 2022 Jul 12:e202209785. Epub 2022 Jul 12.

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Haidian District, Beijing, 100191, China.

The crystal structures of cattleyene synthase (apo-CyS), and CyS complexed with geranylgeranyl pyrophosphate (GGPP) were solved. The CyS variant exhibited an increased production of cattleyene and other diterpenes with diverse skeletons. Its structure showed a widened active site cavity explaining the relaxed selectivity. Isotopic labeling experiments revealed a remarkable cyclization mechanism involving several skeletal rearrangements for one of the novel diterpenes.
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http://dx.doi.org/10.1002/anie.202209785DOI Listing
July 2022

Comparative genome anatomy reveals evolutionary insights into a unique amphitriploid fish.

Nat Ecol Evol 2022 Jul 11. Epub 2022 Jul 11.

State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, University of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

Triploids are rare in nature because of difficulties in meiotic and gametogenic processes, especially in vertebrates. The Carassius complex of cyprinid teleosts contains sexual tetraploid crucian carp/goldfish (C. auratus) and unisexual hexaploid gibel carp/Prussian carp (C. gibelio) lineages, providing a valuable model for studying the evolution and maintenance mechanism of unisexual polyploids in vertebrates. Here we sequence the genomes of the two species and assemble their haplotypes, which contain two subgenomes (A and B), to the chromosome level. Sequencing coverage analysis reveals that C. gibelio is an amphitriploid (AAABBB) with two triploid sets of chromosomes; each set is derived from a different ancestor. Resequencing data from different strains of C. gibelio show that unisexual reproduction has been maintained for over 0.82 million years. Comparative genomics show intensive expansion and alterations of meiotic cell cycle-related genes and an oocyte-specific histone variant. Cytological assays indicate that C. gibelio produces unreduced oocytes by an alternative ameiotic pathway; however, sporadic homologous recombination and a high rate of gene conversion also exist in C. gibelio. These genomic changes might have facilitated purging deleterious mutations and maintaining genome stability in this unisexual amphitriploid fish. Overall, the current results provide novel insights into the evolutionary mechanisms of the reproductive success in unisexual polyploid vertebrates.
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http://dx.doi.org/10.1038/s41559-022-01813-zDOI Listing
July 2022

Exploring miRNA-mRNA regulatory modules responding to tannic acid stress in (Graeser) (Lepidoptera: Notodontidae) via small RNA sequencing.

Bull Entomol Res 2022 Jul 12:1-12. Epub 2022 Jul 12.

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, People's Republic of China.

MicroRNAs (miRNAs) are small noncoding RNAs (sRNAs) that regulate gene expression by inhibiting translation or degrading mRNA. Although the functions of miRNAs in many biological processes have been reported, there is currently no research on the possible roles of miRNAs in (Graeser) involved in the response of plant allelochemicals. In this article, six sRNA libraries (three treated with tanic acid and three control) from were constructed using Illumina sequencing. From the results, 312 known and 43 novel miRNAs were differentially expressed. Notably, some of the most abundant miRNAs, such as miR-432, miR-541-3p, and miR-4448, involved in important physiological processes were also identified. To better understand the function of the targeted genes, we performed Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The results indicated that differentially expressed miRNA targets were involved in metabolism, development, hormone biosynthesis, and immunity. Finally, we visualized a miRNA-mRNA regulatory module that supports the role of miRNAs in host-allelochemical interactions. To our knowledge, this is the first report on miRNAs responding to tannic acid in . This study provides indispensable information for understanding the potential roles of miRNAs in and the applications of these miRNAs in management.
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http://dx.doi.org/10.1017/S0007485322000359DOI Listing
July 2022

Application of Accelerometer to Monitor Students' Exercise Load in 50 m Round Trip.

Authors:
Hao Li Meng Xu

Comput Intell Neurosci 2022 29;2022:3142677. Epub 2022 Jun 29.

Department of Physical Education, Honam University, Gwangju 62397, Metropolitan City, Republic of Korea.

With the further advancement of microelectronics innovation and sensors, sensors can be broadly implanted in cell phone gadgets, compact gadgets, and so forth. The utilization of speed increase sensors for human running checking has expansive application possibilities. From one perspective, the everyday development of the human body is firmly connected with the physical and emotional wellness of the person. Observing the day-to-day developments of the human body is of incredible importance in planning a logical running activity plan and working on actual wellbeing. On the other hand, it is also of practical value to monitor human abnormal movements. This kind of abnormal movement caused by accidental falls can bring certain harm to the human body. Real-time monitoring of the fall can provide timely assistance to the person and reduce the risk brought by the fall. This article analyzes and summarizes the research theories and common research methods in the field of 50 m round-trip movement monitoring based on the acceleration sensor. According to the process of 50 m round-trip movement pattern recognition, the data collection, preprocessing, feature extraction, and selection of 50 m round-trip movement are evaluated. The classification and recognition of each module were analyzed. This article proposes a human body motion recognition mechanism based on acceleration sensors by looking at the three trademark upsides, the wavefront edge, wavefront limit, and time stretch between the pinnacle and valley of the speed increase sensor vertical information waveform, and joining the rule of choice tree order to accomplish the activities of hunching down, taking off, and running. To get an accurate recognizable proof and recognize ways of behaving, a human fall identification calculation is proposed. This calculation removes human movement attributes throughout the fall and focuses on four sorts of falls: forward fall, reverse fall, left fall, and right fall by utilizing the connection of the three tomahawks of the speed increase sensor. The trial results show that the normal right acknowledgment pace of the human body's 50 m full-circle running way of behaviour is more than 90%, which has specific useful application esteem.
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http://dx.doi.org/10.1155/2022/3142677DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259259PMC
July 2022

Genome-Wide Identification of PLATZ Transcription Factors in L. and Their Expression Characteristics During Seed Development.

Front Plant Sci 2022 23;13:946194. Epub 2022 Jun 23.

Key Laboratory of Forestry Genetics and Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.

Plant AT-rich protein and zinc-binding protein (PLATZ) is a class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. PLATZ plays an important role in seed development, water tolerance, and cell proliferation in early plant growth. In this study, 11 s were identified from the ginkgo genome with complete PLATZ-conserved domains, which represents a smaller number compared with angiosperms. Multi-species phylogenetic analysis showed that PLATZ genes were conserved in seed plants, and the 11 members were represented by four groups, among which groups I and II were closely related. Analysis of gene structures, sequence module characteristics, and expression patterns showed that s were similar within and differed between groups. RNA-seq and qRT-PCR results showed that s had distinct expression patterns. Most genes were associated with seed development, among which six genes were highly related. Subcellular localization experiments showed that six GbPLATZ proteins related to seed development were localized in the nucleus, suggesting that they might function as traditional transcription factors. This study provides a basis for understanding the structural differentiation, evolutionary characteristics, expression profile, and potential functions of PLATZ transcription factors in .
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http://dx.doi.org/10.3389/fpls.2022.946194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262033PMC
June 2022

Analysis of Related Factors of Coping Styles in Infertile Patients in Central China.

Patient Prefer Adherence 2022 2;16:1605-1612. Epub 2022 Jul 2.

Department of Reproductive Medicine, the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, People's Republic of China.

Objective: To investigate the psychosocial factors, including personality characteristics, coping style, social support and alexithymia of infertile patients, and explore the relevant factors regarding the medical coping style choice of infertile patients in central China.

Methods: Sixty infertile patients in the reproductive medicine centre in our hospital were investigated using a self-made general situation questionnaire; internal control, powerful others and opportunities scale; social support rating scale; and medical coping questionnaire, and the data was analysed.

Results: Of the 60 infertile patients, 57 (95%) were female, with an average age of 27.85 ± 14.52 years old, and 42 (70%) had secondary infertility. The score of the face dimension was lower than the norm (19.17 ± 2.18 vs 19.43 ± 3.82, P = 0.012), and those of the avoidance (15.98 ± 2.43 vs 14.41 ± 2. 94, P = 0.030) and yield (9.48 ± 3.19 vs 8.83 ± 3.19, P = 0.012) dimensions were higher than the norm. The multiple linear regression analysis showed that internal control and the utilisation of support were influencing factors for the coping style (R = 0.271, adjusted R = 0.144, F = 9.827, < 0.001), and internal control was the influencing factor for the avoidance coping style (R = 0.024, adjusted R = 0.021, F = 3.363, = 0.014). The education level, opportunity and utilisation of support factors influenced the yield dimension (R = 0.087, adjusted R = 0.076, F = 7.749, < 0.001).

Conclusion: Considering the association between the coping styles and psychosocial factors, it was recommended to formulate targeted intervention strategies based on these influencing factors to guide patients to adopt positive coping styles and achieve good outcomes.
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http://dx.doi.org/10.2147/PPA.S364345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258771PMC
July 2022

Detection of a novel panel of 24 genes with high frequencies of mutation in gastric cancer based on next-generation sequencing.

World J Clin Cases 2022 May;10(15):4761-4775

Department of Oncology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.

Background: Gastric cancer is a leading cause of cancer-related mortality worldwide. Many somatic mutations have been identified based on next-generation sequencing; they likely play a vital role in cancer treatment selection. However, next-generation sequencing has not been widely used to diagnose and treat gastric cancer in the clinic.

Aim: To test the mutant gene frequency as a guide for molecular diagnosis and personalized therapy in gastric cancer by use of next-generation sequencing.

Methods: We constructed a panel of 24 mutant genes to detect somatic nucleotide variations and copy number variations based on a next-generation sequencing technique. Our custom panel included high-mutation frequency cancer driver and tumour suppressor genes. Mutated genes were also analyzed using the cBioPortal database. The clinical annotation of important variant mutation sites was evaluated in the ClinVar database. We searched for candidate drugs for targeted therapy and immunotherapy from the OncoKB database.

Results: In our study, the top 16 frequently mutated genes were TP53(58%), ERBB2(28%), BRCA2 (23%), NF1 (19%), PIK3CA (14%), ATR (14%), MSH2 (12%), FBXW7 (12%), BMPR1A (12%), ERBB3 (11%), ATM (9%), FGFR2 (8%), MET (8%), PTEN (6%), CHD4 (6%), and KRAS (5%). TP53 is a commonly mutated gene in gastric cancer and has a similar frequency to that in the cBioPortal database. 33 gastric cancer patients (51.6%) with microsatellite stability and eight patients (12.5%) with microsatellite instability-high were investigated. Enrichment analyses demonstrated that high-frequency mutated genes had transmembrane receptor protein kinase activity. We discovered that BRCA2, PIK3CA, and FGFR2 gene mutations represent promising biomarkers in gastric cancer.

Conclusion: We developed a powerful panel of 24 genes with high frequencies of mutation that could detect common somatic mutations. The observed mutations provide potential targets for the clinical treatment of gastric cancer.
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http://dx.doi.org/10.12998/wjcc.v10.i15.4761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198883PMC
May 2022

Carbon Nanotubes Grown on the Carbon Fibers to Enhance the Photothermal Conversion toward Solar-Driven Applications.

ACS Appl Mater Interfaces 2022 Jul 7;14(28):32404-32411. Epub 2022 Jul 7.

Institute of Low-Dimensional Materials Genome Initiative, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, China.

Photothermal conversion is a directly, sustainable, and green path to use solar energy and the one of the most important keys is the photothermal conversion material. How to obtain the durable and effective material for photothermal conversion with low cost and facile preparation is still a great challenge. In this work, the carbon nanotubes (CNTs) are grown on the carbon fibers (CFs) via the catalysis of trapped Fe and Co. The absorption of the as-prepared CFs/CNTs illustrate the enhancement from the visible light to the near-infrared light range. The photothermal conversion characterization shows the grown CNTs promoting the higher surface temperature and the highest temperature reaches to about 325 °C under 10 sun irradiations. The water evaporation on the CFs/CNTs is measured 1.40 ± 0.03 kg·cm·h under 1 sun irradiation and the water evaporation rate is also found depending on the irradiation density. The photothermal conversion applications and the water evaporation under natural irradiation also reveal the suitable candidate of the CFs/CNTs for photothermal conversion application. This work provides a facile path to obtain effective carbon-based materials for photothermal application.
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http://dx.doi.org/10.1021/acsami.2c07970DOI Listing
July 2022

Effect of Repairing Tendon and Ligament Injury of Wushu Athletes by Medical Image.

Comput Intell Neurosci 2022 24;2022:8494734. Epub 2022 Jun 24.

Department of Physical Education, Honam University, Gwangju Metropolitan City 62397, Republic of Korea.

Medical imaging can be used as a medical aid for diagnosis and treatment, and color Doppler ultrasound can also be used in life science research as a scientific research method. Wushu is a traditional sport in China, which has a long history of development. Martial arts are a very good fitness project, but different from ordinary people, professional martial arts athletes are often accompanied by a variety of sports injuries, and tendon ligament injury is one of the most common injuries. At present, there are many treatment plans for tendon and ligament injury, but there are few researches on the repair effect of tendon and ligament injury. This paper will take this as the main research purpose for in-depth study. In view of the problem that ligament injury is not easy to observe, this paper will use GE Lightspeed 64 row spiral CT as the main observation tool and use the method of hospital image observation to compare and analyze the repair effect of tendon and ligament injury of Wushu athletes. In this experiment, 88 professional Wushu athletes were gathered as experimental samples. After preliminary screening, 110 cases of ligament injury were counted. After analyzing the abnormal changes of tissue or structure, Lysholm, and IKDC treatment effect score data, this paper believes that, for type I patients, only conservative treatment can achieve good results. However, in the more serious and complex type II patients, local fixation is used after the onset of the disease, and very serious patients can achieve good results through surgical treatment. Postoperative care is also important, which helps reduce complications. This experiment has achieved ideal results and has played a blank role in the research of the repair effect of tendon and ligament injury of Wushu athletes at home and abroad.
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http://dx.doi.org/10.1155/2022/8494734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249462PMC
July 2022

EBF1-mediated up-regulation of lncRNA FGD5-AS1 facilitates osteosarcoma progression by regulating miR-124-3p/G3BP2 axis as a ceRNA.

J Orthop Surg Res 2022 Jun 27;17(1):332. Epub 2022 Jun 27.

Orthopaedic Medical Center, the Second Hospital of Jilin University, No. 218, Ziqiang Street, Changchun, 130041, Jilin, China.

Background: As a skeletal malignancy, osteosarcoma has high incidence among primary malignant bone tumors. With increasing researches on molecules which mediate cancer progression, molecular mechanism has gradually become the pivot of osteosarcoma research and treatment.

Aim: Our study aimed at investigating the function of G3BP stress granule assembly factor 2 (G3BP2), which is an oncogene for breast cancer (BC) and prostate cancer but remains unknown in osteosarcoma cells.

Methods: Related gene expression was confirmed by RT-qPCR. Functional assays including immunofluorescence (IF), colony formation, transferase-mediated dUTP nick-end labeling (TUNEL) as well as transwell assays were utilized to test the cell biological process caused by the genes. Meanwhile, RNA pull-down assay, along with luciferase reporter and RNA immunoprecipitation (RIP) assays, was utilized to detect the interaction G3BP2, miR-124-3p and FGD5 antisense RNA 1 (FGD5-AS1) may exert on the regulation of osteosarcoma cells.

Results: G3BP2 was with high expression in osteosarcoma cells, and it aggravated the malignant cell behaviors in osteosarcoma. Additionally, miR-124-3p was verified to negatively regulate G3BP2 expression in osteosarcoma cells. Moreover, lncRNA FGD5-AS1 was predicted and testified to be the sponge of miR-124-3p and modulated G3BP2 expression positively. Subsequently, FGA5-AS1 accelerated osteosarcoma cell proliferation through up-regulating G3BP2. Furthermore, we identified EBF transcription factor 1 (EBF1) as the transcription factor for FGA5-AS1, and EBF1 served as a tumor facilitator in osteosarcoma cells.

Conclusion: EBF1 induced-FGA5-AS1 aggravated osteosarcoma cell malignancy by targeting miR-124-3p and G3BP2.
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http://dx.doi.org/10.1186/s13018-022-03181-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235248PMC
June 2022

Corrigendum to "PCAF inhibits hepatocellular carcinoma metastasis by inhibition of epithelial-mesenchymal transition by targeting Gli-1" [Cancer Letters 375 (1) (2016 May 28) 190-198].

Cancer Lett 2022 Sep 24;543:215806. Epub 2022 Jun 24.

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China. Electronic address:

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http://dx.doi.org/10.1016/j.canlet.2022.215806DOI Listing
September 2022

A Temperature-Sensitive Polymeric Rheology Modifier Used in Water-Based Drilling Fluid for Deepwater Drilling.

Gels 2022 May 30;8(6). Epub 2022 May 30.

Key Laboratory of Unconventional Oil & Gas Development, China University of Petroleum (East China), Ministry of Education, Qingdao 266580, China.

Rheology modifiers are essential for the flat rheology of water-based drilling fluids in deepwater. The low temperature thickening of deepwater water-based drilling fluids results in dramatic rheological changes in the 20-30 °C range. To address such problems, NIPAM with a self-polymerized product LCST of 32-35 °C was selected as the main body for synthesis. While introducing the hydrophilic monomer AM to enhance the thickening properties, the hydrophobic monomer BA was selected to reduce the LCST of the product. In this paper, a temperature-sensitive polymeric rheology modifier (PNBAM) was synthesized by emulsion polymerization using -isopropyl acrylamide, acrylamide, and butyl acrylate as monomers. The PNBAM was characterized using infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and nuclear magnetic resonance hydrogen spectroscopy (NMR). The rheological properties, temperature resistance, and salt resistance of PNBAM in the base fluid (BF) were tested. The performance of PNBAM in the drilling fluid system was also evaluated, and a water-based drilling fluid system of flat rheology for deepwater was formulated. The rheological modification mechanism of PNBAM was analyzed by turbidity analysis, particle size analysis, and zeta analysis. Experimental results show that PNBAM has good rheological properties. PNBAM is temperature resistant to 150 °C, salt-resistant to 30 wt%, and calcium resistant to 1.0 wt%. PNBAM also has good flat rheology characteristics in drilling fluid systems: AV:AV = 1.27, PV:PV = 1.19. Mechanistic analysis showed that the LCST (Lower Critical Solution Temperature) of 0.2 wt% PNBAM in an aqueous solution was 31 °C. Through changes in hydrogen bonding forces with water, PNBAM can regulate its hydrophilic and hydrophobic properties before and after LCST, which thus assists BF to achieve a flat rheological effect. In summary, the temperature-sensitive effect of PNBAM has the property of enhancing with increasing temperature. While the tackifying effect of conventional rheology modifiers diminishes with increasing temperature, the temperature-sensitive effect of PNBAM gives it an enhanced thickening effect with increasing temperature, making it a more novel rheology modifier compared to conventional treatment additives. After LCST, compared to conventional rheology modifiers (XC), PNBAM has a more pronounced thermo-thickening effect, improving the main rheological parameters of BF by more than 100% or even up to 200% (XC less than 50%). This contributes to the flat rheology of drilling fluids. PNBAM has good application prospects and serves as a good reference for the development of other rheology modifiers.
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http://dx.doi.org/10.3390/gels8060338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222916PMC
May 2022

Rapid titration of recombinant baculoviruses based on NanoLuc secretion in early infection.

J Virol Methods 2022 Sep 18;307:114565. Epub 2022 Jun 18.

Department of Biopharmacy, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China. Electronic address:

The baculovirus expression vector system has become a powerful tool for recombinant protein production and gene delivery. However, existing titration methods for baculovirus are not economical in terms of test time and cost. A titration method based on NanoLuc secretion that allows for titration of recombinant baculoviruses at 4 h post infection (hpi) is described. In the assay, the envelop protein GP64 signal peptide-guided NanoLuc was secreted into the culture medium in proportion to the virus amount during early infection under combined control by the homologous region 5 (hr5) enhancer and the promoter of immediate early gene 1 (ie1) plus L21. Two timepoint standard curves of luciferase activity to virus titers of 5-9 logs were established with excellent linearity and correlation coefficients (slope = 1.050, R ≥ 0.9969) using a secretory Nanoluc (secrNluc) - inserted standard baculovirus. Through the assay, the titers of three recombinant viruses prepared independently were calculated by directly measuring luciferase activity in the supernatant at 4 and 6 hpi, with greater accuracy compared to the endpoint dilution assay. These results show the efficacy of this proposed method as a streamlined assay for rapidly titrating recombinant baculoviruses.
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http://dx.doi.org/10.1016/j.jviromet.2022.114565DOI Listing
September 2022

Graph-in-Graph Convolutional Network for Hyperspectral Image Classification.

IEEE Trans Neural Netw Learn Syst 2022 Jun 20;PP. Epub 2022 Jun 20.

With the development of hyperspectral sensors, accessible hyperspectral images (HSIs) are increasing, and pixel-oriented classification has attracted much attention. Recently, graph convolutional networks (GCNs) have been proposed to process graph-structured data in non-Euclidean domains and have been employed in HSI classification. But most methods based on GCN are hard to sufficiently exploit information of ground objects due to feature aggregation. To solve this issue, in this article, we proposed a graph-in-graph (GiG) model and a related GiG convolutional network (GiGCN) for HSI classification from a superpixel viewpoint. The GiG representation covers information inside and outside superpixels, respectively, corresponding to the local and global characteristics of ground objects. Concretely, after segmenting HSI into disjoint superpixels, each one is converted to an internal graph. Meanwhile, an external graph is constructed according to the spatial adjacent relationships among superpixels. Significantly, each node in the external graph embeds a corresponding internal graph, forming the so-called GiG structure. Then, GiGCN composed of internal and External graph convolution (EGC) is designed to extract hierarchical features and integrate them into multiple scales, improving the discriminability of GiGCN. Ensemble learning is incorporated to further boost the robustness of GiGCN. It is worth noting that we are the first to propose the GiG framework from the superpixel point and the GiGCN scheme for HSI classification. Experiment results on four benchmark datasets demonstrate that our proposed method is effective and feasible for HSI classification with limited labeled samples. For study replication, the code developed for this study is available at https://github.com/ShuGuoJ/GiGCN.git.
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http://dx.doi.org/10.1109/TNNLS.2022.3182715DOI Listing
June 2022

Risk Factors for Cardiac Complications in Patients With Pheochromocytoma and Paraganglioma: A Retrospective Single-Center Study.

Front Endocrinol (Lausanne) 2022 1;13:877341. Epub 2022 Jun 1.

Department of Cardiology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

Background: Catecholamine excess arising from pheochromocytomas and paragangliomas (PPGLs) can cause a wide spectrum of cardiac manifestations. Although there are reviews of reported cases, these reviews lack detailed data, which makes it impossible to perform an accurate analysis. In this study, we conducted a comprehensive analysis of cardiovascular complications (CCs), including PPGL-related myocardial injury, cardiogenic shock, and arrhythmias requiring antiarrhythmic therapy, in a large cohort of patients with PPGL.

Methods: We retrospectively analyzed the clinical data of consecutive patients with PPGL admitted between January 2018 and June 2020. The prevalence and the characteristics of patients with CCs were investigated. Moreover, comparisons were made between patients with and without CCs.

Results: Compared with the non-CC group, the percentage of men was significantly lower (14/41 vs.92/175, 34.1% vs. 52.6%, p = 0.034) and the proportion of patients with paroxysmal hypertension was significantly higher (13/41 vs.29/173, 31.7% vs.16.8%, p = 0.03) in the CC group. More patients showed excessive sweating (19/41 vs 64/175, 46.3% vs. 24.0%, p = 0.004) and PPGL crisis (7/41 vs. 10/175, 17.1% vs.5.7%, p=0.035) in the CC group. In terms of laboratory findings, higher white blood cell [7.36 (6.49, 20.23) vs. 5.95 (5.1, 6.97)×10/L, p<0.001] and platelet [339.28 ± 108.54 vs. 250.66 ± 70.83(×10/L), p = 0.021] counts were more common in the CC group. There was also a higher prevalence of combination-producing PPGL in the CC group (13/24 vs.20/149, 54.2% vs.13.4%, p<0.001). However, the tumor size, invasive behavior on histology, and hemorrhage or necrosis on histology did not differ between the two groups. Platelet count [odds ratio (OR): 1.009; 95% confidence interval (CI) 1.001-1.016; p=0.023] and combination-secreting PPGL (OR: 5.009; 95% CI 1.365-18.38; p=0.015) are independent risk factors for CCs in patients with PPGL.

Conclusions: In patients with PPGL, even in the absence of signs and symptoms of CCs, a work up of cardiology should be strongly considered. Importantly, if patients with PPGLs have higher platelet counts and the combination-secreting pattern, they are more likely to have CCs. Thus, a careful cardiac evaluation should be performed.
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http://dx.doi.org/10.3389/fendo.2022.877341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9199364PMC
June 2022

Application Potential of Baijiu Non- Yeast in Winemaking Through Sequential Fermentation With .

Front Microbiol 2022 30;13:902597. Epub 2022 May 30.

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.

To explore the potential application of non- yeasts screened from Baijiu fermentation environment in winemaking, the effect of four Baijiu non- yeasts (two and two ) sequentially fermented with on the physicochemical parameters and volatile compounds of wine was analyzed. The results indicated that there was no obvious antagonism between and or in sequential fermentations, and all strains could be detected at the end of alcoholic fermentation. Compare with pure fermentation, sequential fermentations significantly reduced higher alcohols, fatty acids, and ethyl esters and increased acetate esters; sequential fermentations reduced the contents of C6 alcohols, total higher alcohols, fatty acids, and ethyl esters and significantly increased the contents of acetate esters (especially ethyl acetate and 3-methylbutyl acetate). Sequential fermentation of Baijiu non- yeast and improved the flavor and quality of wine due to the higher ester content and lower concentration of higher alcohols and fatty acids, non- yeasts selected from Baijiu fermentation environment have potential applications in winemaking, which could provide a new strategy to improve wine flavor and quality.
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http://dx.doi.org/10.3389/fmicb.2022.902597DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196592PMC
May 2022

Curcumae longae Rhizoma (Jianghuang) extract reverses the 5-Fluoruracil resistance in colorectal cancer cells via TLR4/PI3K/Akt/mTOR pathway.

Clin Res Hepatol Gastroenterol 2022 Jun 13;46(9):101976. Epub 2022 Jun 13.

Anorectal Department, Dalian Municipal Central Hospital, No.826, Southwest Road, Shahekou District, Dalian, Liaoning 116033, China. Electronic address:

Objectives: Intensive efforts have been made in the area of identifying drug resistance modulators from traditional Chinese medicine. Various natural plant extracts have been reported for their reversal effect of drug resistance in cancers. This study was to assess the reversal potential of Curcumae longae Rhizoma extract (CLRE) in 5-Fluorouracil (5-Fu) resistance to colon cancer and explore its underly mechanism.

Methods: Increased concentrations of 5-Fu were used to culture SW480. A series of concentrations of CLRE were used to treat the 5-Fu resistant SW480 cells. WST-8 assay was used to detect the cell viability. Cell apoptosis was assessed by SuperView™ 488 Caspase-3 Assay Kit. The quantification of mentioned factors was archived by RT-qPCR. Network pharmacology analysis was used to explore the target of CLRE.

Results: 5-Fu resistant cell line (SW480/5-FuR) was established. The IC50 value of CLRE against SW480/5-FuR was 181.0 ± 14.12 µg/ml. CLRE can resensitize the SW480/5-FuR to 5-Fu by inhibiting cell growth. The combination treatment (CLRE+5-Fu) induced cell apoptosis via inhibition of bcl-2 and activation of caspase-3 and bax. Three active ingredients from CLRE were identified. TLR4 was targeted by these three ingredients and linked these ingredients to PI3K/Akt/mTOR pathway. The levels of TLR4, PI3K, AKT1, and mTORC1 mRNA were decreased when 5-Fu was combined with CLRE.

Conclusions: CLRE could reverse 5-Fu resistance in colon cancer by inactivating TLR4/PI3K/AKT/mTORC1 pathway. This finding might provide a molecular basis and a valuable direction for further clinical applications and research for treating 5-Fu resistant colon cancer.
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http://dx.doi.org/10.1016/j.clinre.2022.101976DOI Listing
June 2022
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