Publications by authors named "Xu Jin"

1,692 Publications

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Ferroptosis-related lncRNA pairs to predict the clinical outcome and molecular characteristics of pancreatic ductal adenocarcinoma.

Brief Bioinform 2021 Sep 22. Epub 2021 Sep 22.

Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.

Ferroptosis is a form of regulated cell death initiated by oxidative perturbations that can be blocked by iron chelators and lipophilic antioxidants, and ferroptosis may be the silver bullet treatment for multiple cancers, including immunotherapy- and chemotherapy-insensitive cancers such as pancreatic ductal adenocarcinoma (PDAC). Numerous studies have noted that long non-coding RNAs (lncRNAs) regulate the biological behaviour of cancer cells by binding to DNA, RNA and protein. However, few studies have reported the role of lncRNAs in ferroptosis processes and the function of ferroptosis-associated lncRNAs. The primary objective of the present study was to identify ferroptosis-related lncRNAs using bioinformatic approaches combined with experimental validation. The second objective was to construct a prognostic model to predict the overall survival of patients with PDAC. The present study identified ferroptosis-related lncRNAs using a bioinformatic approach and validated them in an independent pancreatic cancer cohort from Fudan University Shanghai Cancer Center. The lncRNA SLCO4A1-AS1 was identified as a novel molecule mediating ferroptosis resistance in vitro. A novel algorithm was used to construct a '0 or 1' matrix-based prognosis model, which showed promising diagnostic accuracy for potential clinical translation (area under the curve = 0.89 for the 2-year survival rate). Notably, molecular subtypes classified by the risk scores of the model did not belong to any previously reported subtypes of PDAC. The immune microenvironment, metabolic activities, mutation landscape and ferroptosis sensitivity were significantly distinct between patients with different risk scores. Sensitivity (IC50) to 30 common anticancer drugs was analysed between patients with different risks, and imatinib and axitinib were found to be potential drugs for the treatment of patients with lower risk scores. Overall, we developed an accurate prognostic model based on the expression patterns of ferroptosis lncRNAs, which may contribute greatly to the evaluation of patient prognosis, molecular characteristics and treatment modalities and could be further translated into clinical applications.
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http://dx.doi.org/10.1093/bib/bbab388DOI Listing
September 2021

Analytical and clinical evaluation of a novel assay for measurement of interleukin 6 in human whole blood samples.

J Clin Lab Anal 2021 Sep 20:e24011. Epub 2021 Sep 20.

Department of Clinical Laboratory, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai, China.

Background: Interleukin 6 assays are useful in early detection of infections and risk stratification of critically ill patients, so an assay with a short turnaround-time and near-patient use is preferred. This study evaluated the performance of a new interleukin 6 assay, Pylon IL-6 assay, and explored its potential use in near-patient settings.

Methods: We carried out imprecision, linearity and comparison studies using serum and plasma samples according to CLSI EP guidelines. The stability of whole blood samples during storage was assessed. Furthermore, whole blood samples from pediatric patients with suspected infection were measured to evaluate the assay's diagnostic performance.

Results: The within-run CVs and total CVs of Pylon IL-6 assay were determined as 1.8% and 3.0% at 159.3 pg/ml and 3.5% and 4.7% at 8009.9 pg/ml, respectively. The method showed linearity between 1.5 and 42,854 pg/ml. The results of serum samples measured by Pylon assays correlated to those measured by Roche assays, as well as to those of matched whole blood samples measured by Pylon assays. IL-6 in whole blood was found stable for ~8 h at room temperature. Pylon IL-6 results of whole blood samples from 179 pediatric patients with suspected infection showed an AUC of 0.842 in diagnosis of bacterial infection. The turnaround time of Pylon IL-6 assay was only 1 h when using whole blood samples.

Conclusion: The new assay demonstrated performance comparable to those performed on clinical laboratory instruments and can be used in near-patient settings with whole blood to reduce turnaround times.
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http://dx.doi.org/10.1002/jcla.24011DOI Listing
September 2021

Surrogate-Assisted Hybrid-Model Estimation of Distribution Algorithm for Mixed-Variable Hyperparameters Optimization in Convolutional Neural Networks.

IEEE Trans Neural Netw Learn Syst 2021 Sep 20;PP. Epub 2021 Sep 20.

The performance of a convolutional neural network (CNN) heavily depends on its hyperparameters. However, finding a suitable hyperparameters configuration is difficult, challenging, and computationally expensive due to three issues, which are 1) the mixed-variable problem of different types of hyperparameters; 2) the large-scale search space of finding optimal hyperparameters; and 3) the expensive computational cost for evaluating candidate hyperparameters configuration. Therefore, this article focuses on these three issues and proposes a novel estimation of distribution algorithm (EDA) for efficient hyperparameters optimization, with three major contributions in the algorithm design. First, a hybrid-model EDA is proposed to efficiently deal with the mixed-variable difficulty. The proposed algorithm uses a mixed-variable encoding scheme to encode the mixed-variable hyperparameters and adopts an adaptive hybrid-model learning (AHL) strategy to efficiently optimize the mixed-variables. Second, an orthogonal initialization (OI) strategy is proposed to efficiently deal with the challenge of large-scale search space. Third, a surrogate-assisted multi-level evaluation (SME) method is proposed to reduce the expensive computational cost. Based on the above, the proposed algorithm is named surrogate-assisted hybrid-model EDA (SHEDA). For experimental studies, the proposed SHEDA is verified on widely used classification benchmark problems, and is compared with various state-of-the-art methods. Moreover, a case study on aortic dissection (AD) diagnosis is carried out to evaluate its performance. Experimental results show that the proposed SHEDA is very effective and efficient for hyperparameters optimization, which can find a satisfactory hyperparameters configuration for the CIFAR10, CIFAR100, and AD diagnosis with only 0.58, 0.97, and 1.18 GPU days, respectively.
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http://dx.doi.org/10.1109/TNNLS.2021.3106399DOI Listing
September 2021

Non-fasting lipid profile for cardiovascular risk assessments using China ASCVD risk estimator and Europe SCORE risk charts in Chinese participants.

Cardiovasc Diagn Ther 2021 Aug;11(4):991-1001

Department of Cardiovascular Medicine, The Second Xiangya Hospital, Central South University, Changsha, China.

Background: Previous studies have shown that non-fasting lipids have similar values in cardiovascular risk estimation as fasting, but it is not clear whether this could also be applicable to Chinese participants.

Methods: A total of 127 (76 men, 51 women) participants without atherosclerotic cardiovascular diseases (ASCVD) were enrolled in the study. Serum levels of blood lipids were monitored at 0 h, 2 h and 4 h after a daily breakfast. Ten-year cardiovascular disease (CVD) risk was estimated with China ASCVD risk estimator and European SCORE risk charts. Kappa statistic was used to determine agreement among estimators.

Results: China ASCVD risk estimator assessed half of the participants as low risk, while European risk charts assessed half of the participants as moderate risk in the same participants. Reliability analysis in China ASCVD risk estimator and Europe SCORE risk charts based on fasting and or non-fasting lipids profile were relatively high (Kappa =0.731 or 0.718, P<0.001), (Kappa =0.922 or 0.935, P<0.001) (Kappa =0.886 or 0.874, P<0.001), but agreement between two were relatively poor in both fasting and non-fasting states (Kappa =0.339 or 0.300, P<0.001), (Kappa =0.364 or 0.286, P<0.001).

Conclusions: Promoting use of non-fasting lipids in diagnosis, evaluation, and prediction of CVD are feasible. Furthermore, non-fasting lipids could be used in China ASCVD risk estimator to evaluate 10-year risk of ASCVD among Chinese general participants.
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http://dx.doi.org/10.21037/cdt-20-1012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410488PMC
August 2021

Variability in growth and cadmium accumulation capacity among willow hybrids and their parents: implications for yield-based selection of Cd-efficient cultivars.

J Environ Manage 2021 Sep 6;299:113643. Epub 2021 Sep 6.

College of Environmental Science and Forestry, State University of New York, 1 Forestry Dr., Syracuse, NY, 13210, USA.

Growth responses and cadmium (Cd) accumulation in willow cultivars help determine their potential in remediation of Cd-contaminated conditions. Seventeen willow cultivars, including hybrids and their parents, were grown in hydroponic conditions in a greenhouse, and their capacity for Cd tolerance and accumulation was compared. The results showed that shoot length, biomass production, and concentrations of photosynthetic pigments were significantly affected by 10 μM and 30 μM Cd treatments compared with the control. Biomass production varied across all cultivars and treatments, with maximum shoot dry weight in Owasco grown in 10 μM Cd (11.7 ± 4.5 g plant), and minimum in FC187 in 30 μM Cd (0.3 ± 0.1 g plant). Furthermore, shoot growth proved to be more sensitive to Cd than root growth. Cultivars tolerance to Cd stress varied as indicated by tolerance indices (TIs) ranging from 0.13 to 1.81 for shoots, and from 0.49 to 2.63 for roots. Cd accumulation also differed across treatments and cultivars, with average concentration of 217.49 μg. g in shoots and 478.47 μg. g in roots. Total amounts of Cd in all plant parts ranged from 38.98 to 4116.05 μg per plant, and cultivars SX64, Cicero, and Owasco exhibited a translocation factor (TF) of greater than 1. The correlation and path coefficient analyses demonstrated that shoot biomass reflected Cd transport and phytoextraction ability in selected willow cultivars. Our results also revealed that cultivars with higher leaf Cd concentration could be used as Cd-efficient parents to generate hybrids for Cd phytoextraction. Cultivars with higher biomass production translocated and accumulated more Cd in their aerial parts, and this finding will facilitate yield-based selection of candidates for Cd phytoextraction and for bioenergy production.
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http://dx.doi.org/10.1016/j.jenvman.2021.113643DOI Listing
September 2021

N-Glycosylation and enzymatic activity of the rHuPH20 expressed in Chinese hamster ovary cells.

Anal Biochem 2021 Sep 11;632:114380. Epub 2021 Sep 11.

State Key Laboratory of Antibody Medicine and Targeted Therapy, Shanghai, 201203, China; NMPA Key Laboratory for Quality Control of Therapeutic Monoclonal Antibodies, Shanghai, 201203, China; School of Pharmacy, Liaocheng University, Liaocheng, 252000, China. Electronic address:

rHuPH20, a neutral pH-active hyaluronidase that degrades glycosaminoglycans under physiologic conditions, has six potential N-glycosylation sites. In this report, the rHuPH20 expressed in Chinese hamster ovary (CHO) cells was analyzed and characterized using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Removal of the N-linked glycans from rHuPH20 with PNGase F shifted the molecular weight from 66 kDa to approximately 52 kDa, its deduced molecular weight based on sequence analysis, suggesting that most, if not all, of the potential N-glycosylation sites are linked to oligosaccharides. Then the N-linked glycans released from the rHuPH20 by PNGase F were characterized by UPLC-FLR-MS, and the six N-glycosylation sites of the rHuPH20 were identified and characterized by UPLC-MS/MS at peptide levels. Subsequently, we found that the rHuPH20 increased the dispersion of locally subcutaneous injected drugs and the in vitro and in vivo bioactivity were decreased significantly after PNGase F treatment. In particular, rHuPH20 significantly augmented the absolute bioavailability of locally subcutaneous injected large protein therapeutics, while the bioavailability decreased after being digested by PNGase F. These results demonstrated that N-glycosylation is important for the bioactivity of the rHuPH20.
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http://dx.doi.org/10.1016/j.ab.2021.114380DOI Listing
September 2021

Efficacy and economics evaluation of seed rhizome treatment combined with preplant soil fumigation on ginger soilborne disease, plant growth and yield promotion.

J Sci Food Agric 2021 Sep 12. Epub 2021 Sep 12.

Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Background: Ginger (Zingiber officinale Roscoe) is widely planted around the world. Due to continuous planting, ginger is seriously affected by soilborne fungi, bacteria and nematodes. Although preplant soil fumigation is an effective prevention strategy of soilborne diseases, individual fumigant and technology could not provide effective control of ginger soilborne disease. In our research, different combinations of soil fumigants and seed rhizome treatments were evaluated, by monitoring the soil pathogens population, ginger growth, yield and estimation of economic benefits.

Results: Soil fumigation effectively reduced the population of soilborne pathogens, and chloropicrin had better control effect on soilborne pathogens than dazomet. Preplant soil fumigation and seed rhizome treatment not only provide good control of soilborne disease, but also reduced the incidence of plant foliar pest and disease. Average yield increase rate of seed rhizome treatment was 12.0%, the highest yield increase was 24.4%. The average cost of seed rhizome treatment only increased by about 2.86%, but net revenue increase rate of seed rhizome treatment reached up to 19.1%.

Conclusion: Seed rhizome treatment is a very cost-effective soilborne disease control technology. In the management of soilborne diseases, the combined application of soil fumigation and seed rhizome treatment can reduce the risk of crops infected by soilborne diseases, and ensure high and stable crop yields. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jsfa.11526DOI Listing
September 2021

Controlling phytoplankton blooms in a canyon-shaped drinking water reservoir via artificial and induced natural mixing: Taxonomic versus functional groups.

Chemosphere 2021 Aug 20;287(Pt 2):131771. Epub 2021 Aug 20.

Lijiahe Reservoir Management Co., Ltd., Xi'an, 710016, China.

Water-lifting aerators (WLAs) were often deployed in reservoirs to achieve artificial mixing (WLA activation) and induced (natural) mixing (early occurrence of complete natural mixing after WLA deactivation) for water quality improvement. Here, the mechanisms controlling phytoplankton growth via artificial and induced mixing were explored using a combination of taxonomic and functional classifications based on two-year monitoring data (i.e., non-operational and operational years of the WLAs). Artificial mixing resulted in a decrease of 99.2 % in phytoplankton cell density compared to that of the non-operational year, which continuously diminished to (3.06 ± 0.59) × 10 cells/L during induced mixing. The succession of phytoplankton structure in taxonomic and functional classification levels changed from Cyanobacteria to Chlorophyta and Bacillariophyta, from groups F, J, H1, and L to A and X1, respectively, by comparison of the non-operational and operational years. Decreases in surface water temperature, total phosphorus concentration, and light availability, and increases in mixing depth via artificial and induced mixing were responsible for phytoplankton control, especially for cyanobacterial blooms, depending on a shift in phytoplankton composition from large or colonial, low surface to volume (S/V) to small, high S/V genera. Artificial and induced mixing also improved the trophic/ecological status of the reservoir, from "hyper-eutrophic and bad level" to "light-eutrophic and excellent level", based on an assessment of the trophic level index (TLI) and phytoplankton assemblage (Q) index. This study demonstrates that the suitable combination of artificial and induced mixing plays a crucial role in the maintenance and extension of healthy ecosystems in reservoirs.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131771DOI Listing
August 2021

Temporal dissection of Rai1 function reveals brain-derived neurotrophic factor as a potential therapeutic target for Smith-Magenis syndrome.

Hum Mol Genet 2021 Aug 31. Epub 2021 Aug 31.

Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, McGill University, Québec H3G 1A3, Canada.

Haploinsufficiency of RAI1 is responsible for Smith-Magenis Syndrome (SMS), a childhood neurodevelopmental disorder associated with hyperphagia, obesity, and autistic features. We previously showed that constitutive inactivation of one or both copies of Rai1 in the germline or developing brain induces SMS-like neurobehavioral deficits and obesity in mice. By contrast, the postnatal function of Rai1 is unclear. Here, we globally deleted one or both copies of Rai1 during two postnatal developmental windows by generating an inducible Rai1 knockout mouse model. We found that delayed Rai1 deletion at 3 or 8 weeks of age had no effect on neurobehavioral functions but resulted in adult-onset obesity and decreased expression of brain-derived neurotrophic factor (Bdnf) in the hypothalamus. Remarkably, genetic overexpression of human Bdnf in Rai1 heterozygous mice reversed SMS-like obesity, hyperphagia, metabolic syndrome-like features, and hyposociability. Increasing Bdnf signaling in the paraventricular nucleus of the hypothalamus (PVH) or the ventromedial nucleus of the hypothalamus (VMH) was sufficient to mediate the anti-obesity effect. Our work identifies the function of Rai1 in different temporal windows after birth and provides in vivo evidence that increasing Bdnf signaling is therapeutically effective in a preclinical mouse model of SMS.
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http://dx.doi.org/10.1093/hmg/ddab245DOI Listing
August 2021

Assessing Genetic Overlap and Causality Between Blood Plasma Proteins and Alzheimer's Disease.

J Alzheimers Dis 2021 Aug 25. Epub 2021 Aug 25.

King's College London, Institute of Psychiatry, Psychology and Neuroscience, London, UK.

Background: Blood plasma proteins have been associated with Alzheimer's disease (AD), but understanding which proteins are on the causal pathway remains challenging.

Objective: Investigate the genetic overlap between candidate proteins and AD using polygenic risk scores (PRS) and interrogate their causal relationship using bi-directional Mendelian randomization (MR).

Methods: Following a literature review, 31 proteins were selected for PRS analysis. PRS were constructed for prioritized proteins with and without the apolipoprotein E region (APOE+/-PRS) and tested for association with AD status across three cohorts (n = 6,244). An AD PRS was also tested for association with protein levels in one cohort (n = 410). Proteins showing association with AD were taken forward for MR.

Results: For APOE ɛ3, apolipoprotein B-100, and C-reactive protein (CRP), protein APOE+ PRS were associated with AD below Bonferroni significance (pBonf, p <  0.00017). No protein APOE- PRS or AD PRS (APOE+/-) passed pBonf. However, vitamin D-binding protein (protein PRS APOE-, p = 0.009) and insulin-like growth factor-binding protein 2 (AD APOE- PRS p = 0.025, protein APOE- PRS p = 0.045) displayed suggestive signals and were selected for MR. In bi-directional MR, none of the five proteins demonstrated a causal association (p <  0.05) in either direction.

Conclusion: Apolipoproteins and CRP PRS are associated with AD and provide a genetic signal linked to a specific, accessible risk factor. While evidence of causality was limited, this study was conducted in a moderate sample size and provides a framework for larger samples with greater statistical power.
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http://dx.doi.org/10.3233/JAD-210462DOI Listing
August 2021

HDL cholesterol: A potential mediator of the association between serum levels of a mixture of metals and the risk of aortic dissection in a Chinese population.

Environ Pollut 2021 Aug 9;290:117942. Epub 2021 Aug 9.

State Key Laboratory of Reproductive Medicine, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China; Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, Nanjing Medical University, Nanjing, Jiangsu, China. Electronic address:

Aortic dissection (AD) is a severe cardiovascular disease with a high mortality rate. However, the associations between the serum levels of metals and the risk of AD remain unclear. One hundred twenty-seven patients with AD (type A and B) identified from 2017 to 2019 at the Second Affiliated Hospital of Nanjing Medical University were included; 183 controls that were also included. A logistic regression analysis was performed to determine the associations between serum levels of metals and the risk of AD. Weighted Quantile Sum (WQS) regression and Bayesian Kernel Machine Regression (BKMR) analyses were performed to explore the effects of mixtures of metals on the risk of AD. A linear regression analysis was performed to evaluate the relationships between the serum levels of metals and the white blood cells (WBCs) count and serum lipid levels and blood glucose. We conducted a mediation analysis to explore the contribution rates of WBC counts or serum lipid levels and blood glucose to the association between metal levels and the risk of AD. Exposure to serum levels of Cu (coefficient = 6.33; 95 % CI = 2.52, 10.14; p trend < 0.001) were significantly and positively associated with the risk of AD. In the WQS analysis, Cu (50.3 %), Ni (32.7 %) and Mo (17.1 %) contributed to the AD risk. In the BKMR analysis, Cu and Mo were shown to play important roles in the association with the AD risk. Moreover, serum concentrations of Cu were significantly and inversely associated with HDL-cholesterol levels. HDL-cholesterol levels mediated 7.42 % of the association between serum Cu levels and the prevalence of AD. Our study provided the first evidence that serum levels of mixtures of metals are associated with the AD risk in a Chinese population. Increased concentrations of metals, particularly Cu, may increase the risk of AD by reducing HDL-cholesterol levels.
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http://dx.doi.org/10.1016/j.envpol.2021.117942DOI Listing
August 2021

Apatinib inhibits the proliferation of gastric cancer cells via the AKT/GSK signaling pathway .

Aging (Albany NY) 2021 08 27;13(16):20738-20747. Epub 2021 Aug 27.

Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210000, China.

Gastric cancer (GC) is the third leading cause of cancer-associated mortality globally. Although the diagnosis and therapeutic strategies for GC have improved, the prognosis for advanced gastric cancer (AGC) remains poor. Hence, the present study sought to design a zebrafish model established by microinjecting human MGC-803 GC cell line for studying personalized molecular-targeted cancer therapy. Apatinib, a novel molecular-targeted agent, was evaluated for its efficacy through a comparison among the control groups (no treatment) and subject groups (treatment). Newly formed vessel length and tumor volume were measured in all of the groups for further study. The length of newly formed vessels was obviously shortened after apatinib treatment in the zebrafish model established in this study. Meanwhile, apatinib exhibited the best antitumor growth effect with dose and time dependence by suppressing AKT/GSK3α/β signaling, which may be the mechanism underlying the profound antitumor clinical effect of apatinib. The data indicated that apatinib therapy exerts an anti-angiogenesis effect and it can be recommended as a proper antitumor growth therapy for GC patients. Additionally, zebrafish models could be designed as a potential practical tool to explore new anti-GC cancer drugs.
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http://dx.doi.org/10.18632/aging.203458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436942PMC
August 2021

Mating-Induced Trade-Offs upon Egg Production versus Fertilization and Offspring's Survival in a Sawfly with Facultative Parthenogenesis.

Insects 2021 Aug 2;12(8). Epub 2021 Aug 2.

Yunnan Academy of Biodiversity, Southwest Forestry University, Kunming 650224, China.

Investigation of mating-induced trade-offs between reproduction and survival is conducive to provide evolutionary insights into reproductive strategies and aging. Here, we used RNAseq and bioinformatics to reveal mating-induced changes of genes and pathways related to reproduction and survival in female , a pine defoliator with facultative parthenogenesis and long larval dormancy. Results showed that mating induced substantial downregulation on genes and pathways associated to immunity, stress response, and longevity. However, mating induced divergent reproductive response, with downregulation on genes and pathways related to egg production while upregulation on genes and pathways related to egg fertilization. Considering the nature of limited resources in adults, low fecundity, and egg protection behavior in , we suggest that mating triggers trade-offs between reproduction and survival in this insect and females of this species may have evolved specific strategies to adapt to the environmental and hosts' conditions, e.g., restrict whole fecundity to ensure higher fertilization and offspring's survival. Moreover, mating induced significant responses on genes and pathways that play important roles in vertebrate reproduction while their function in insects are unclear, such as the progesterone-mediated oocyte maturation pathway; the significant regulation after mating suggests that their function may be evolutionarily conserved in animal kingdom.
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http://dx.doi.org/10.3390/insects12080693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8396567PMC
August 2021

Next-generation sequencing yields the complete mitogenome of the stored nut moth, Zeller (Lepidoptera: Pyralidae).

Mitochondrial DNA B Resour 2021 16;6(9):2626-2627. Epub 2021 Aug 16.

Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, PR China.

The stored nut moth, Zeller 1877 (Lepidoptera: Pyralidae), is a pest of stored products. In this study, the whole mitogenome of was identified for the first time by using the next-generation sequencing (NGS) systems. The entire genome is 15,280 bp in length (ACCN: MW135332) consisting of 13 protein-coding genes (PCGs), two ribosomal genes, 22 transfer genes, and an A + T-rich region. Phylogenetic analysis using 13 PCGs of 20 species derived from six moth superfamilies showed that Pyralidae moths are monophyletic. This study can provide essential DNA molecular data for further phylogenetic and evolutionary analysis for Pyralidae family of Lepidoptera order.
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http://dx.doi.org/10.1080/23802359.2021.1915204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381954PMC
August 2021

Comparison of the Reductions in LDL-C and Non-HDL-C Induced by the Red Yeast Rice Extract Xuezhikang Between Fasting and Non-fasting States in Patients With Coronary Heart Disease.

Front Cardiovasc Med 2021 9;8:674446. Epub 2021 Aug 9.

Department of Cardiovascular Medicine, The Second Xiangya Hospital, Central South University, Changsha, China.

Xuezhikang, an extract of red yeast rice, effectively lowers fasting blood lipid levels. However, the influence of Xuezhikang on the non-fasting levels of low-density lipoprotein cholesterol (LDL-C) and non-high-density lipoprotein cholesterol (non-HDL-C) has not been explored in Chinese patients with coronary heart disease (CHD). Fifty CHD patients were enrolled and randomly divided into two groups ( = 25 each) to receive 1,200 mg/d of Xuezhikang or a placebo for 6 weeks as routine therapy. Blood lipids were repeatedly measured before and after 6 weeks of treatment at 0, 2, 4, and 6 h after a standard breakfast containing 800 kcal and 50 g of fat. The serum LDL-C levels significantly decreased, from a fasting level of 3.88 mmol/L to non-fasting levels of 2.99, 2.83, and 3.23 mmol/L at 2, 4, and 6 h, respectively, after breakfast ( < 0.05). The serum non-HDL-C level mildly increased from a fasting level of 4.29 mmol/L to non-fasting levels of 4.32, 4.38, and 4.34 mmol/L at 2, 4, and 6 h post-prandially, respectively, and the difference reached statistical significance only at 4 and 6 h after breakfast ( < 0.05). After 6 weeks of Xuezhikang treatment, the patients had significantly lower fasting and non-fasting serum levels of LDL-C and non-HDL-C ( < 0.05) than at pretreatment. The LDL-C levels were reduced by 27.8, 28.1, 26.2, and 25.3% at 0, 2, 4, and 6 h, respectively, and the non-HDL-C levels were reduced by 27.6, 28.7, 29.0, and 28.0% at 0, 2, 4, and 6 h, respectively, after breakfast. No significant difference was found in the percent reductions in the LDL-C and non-HDL-C levels among the four different time-points. Six weeks of Xuezhikang treatment significantly decreased LDL-C and non-HDL-C levels, with similar percent reductions in fasting and non-fasting states in CHD patients, indicating that the percent change in non-fasting LDL-C or non-HDL-C could replace that in the fasting state for evaluation the efficacy of cholesterol control in CHD patients who are unwilling or unable to fast.
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http://dx.doi.org/10.3389/fcvm.2021.674446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381279PMC
August 2021

RUNX1 Upregulates CENPE to Promote Leukemic Cell Proliferation.

Front Mol Biosci 2021 9;8:692880. Epub 2021 Aug 9.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.

RUNX1 is a Runt family transcription factor that plays a critical role in normal hematopoiesis, including the differentiation and proliferation of hematopoietic cells. RUNX1 mutations, including chromosomal translocations, cause abnormal cell differentiation, but the mutation alone is not sufficient to cause leukemia. In MLL-fusion-induced leukemia, dysregulated wild-type RUNX1 can promote leukemia survival. Nevertheless, the underlying mechanisms of dysregulated wild-type RUNX1 in leukemia development have not been fully elucidated. This study overexpressed and knocked down RUNX1 expression in THP-1 human leukemia cells and CD34 hematopoietic stem/progenitor cells to investigate the biological functions affected by dysregulated RUNX1. Our data indicated RUNX1 facilitated proliferation to promote leukemia cell growth. Furthermore, we demonstrated that RUNX1 knockdown in leukemia cells drastically diminished colony-forming ability. Finally, the RUNX1-knocked down cell depletion phenotype could be rescued by overexpression of CENPE, a cell proliferation gene and a RUNX1 direct target gene. Our results indicate a possible mechanism involving the RUNX1-CENPE axis on promoting leukemic cell growth.
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http://dx.doi.org/10.3389/fmolb.2021.692880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381024PMC
August 2021

Li-ion storage properties of two-dimensional titanium-carbide synthesized via fast one-pot method in air atmosphere.

Nat Commun 2021 Aug 24;12(1):5085. Epub 2021 Aug 24.

College of Materials Science and Engineering, Sichuan University, Chengdu, China.

Structural bidimensional transition-metal carbides and/or nitrides (MXenes) have drawn the attention of the material science research community thanks to their unique physical-chemical properties. However, a facile and cost-effective synthesis of MXenes has not yet been reported. Here, using elemental precursors, we report a method for MXene synthesis via titanium aluminium carbide formation and subsequent in situ etching in one molten salt pot. The molten salts act as the reaction medium and prevent the oxidation of the reactants during the high-temperature synthesis process, thus enabling the synthesis of MXenes in an air environment without using inert gas protection. Cl-terminated TiCT and TiCT MXenes are prepared using this one-pot synthetic method, where the in situ etching step at 700 °C requires only approximately 10 mins. Furthermore, when used as an active material for nonaqueous Li-ion storage in a half-cell configuration, the obtained TiCT MXene exhibits lithiation capacity values of approximately 280 mAh g and 160 mAh g at specific currents of 0.1 A g and 2 A g, respectively.
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http://dx.doi.org/10.1038/s41467-021-25306-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385058PMC
August 2021

Stimulation and Sequestration Mechanism of CO Waterless Fracturing for Continental Tight Oil Reservoirs.

ACS Omega 2021 Aug 4;6(32):20758-20767. Epub 2021 Aug 4.

Beijing Key Laboratory for Greenhouse Gas Storage and CO2-EOR, China University of Petroleum (Beijing), Beijing 102249, China.

CO fracturing is a promising technology for oil field development in tight, continental deposits, with potential advantages of enhanced oil recovery (EOR), CO sequestration, and water conservation. Compared with CO-EOR techniques, such as CO huff and puff and CO flooding, CO can interact with reservoir rock and fluid under higher pressure conditions during fracturing, resulting in CO stimulation and sequestration effects that differ from those that occur during conventional CO-EOR. In this paper, the CO interactions between CO and reservoirs in continental tight oil reservoirs under fracturing conditions are systematically studied through laboratory experiments. The results show that under high pressure, CO effectively changes the pore structure through the extraction of hydrocarbons, dissolution of the rock matrix, and migration of minerals. CO dissolution of the rock matrix can significantly increase the number and complexity of fractures. Furthermore, CO has a higher solubility in formation fluid under high-pressure conditions. Given the higher pressures, CO forms a miscible phase with crude oil, diffuses more deeply into the formation, and reacts fully with the reservoir minerals and fluid during CO fracturing. Accordingly, CO can improve the permeability of the reservoir and flowability of crude oil significantly. Hence, CO fracturing can enhance oil recovery and CO sequestration more effectively. Core displacement experiments indicate that oil recovery of CO soaking process after CO fracturing is 36%, which is 12% and 9% higher than those of CO huff and puff and CO flooding with 5 pore volume, respectively. Field tests show that average oil production after CO fracturing is 1.42 times higher than that after CO flooding, which further validates the advantage of CO fracturing and demonstrates its huge application potential.
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http://dx.doi.org/10.1021/acsomega.1c01059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374915PMC
August 2021

Circular RNA CircEYA3 induces energy production to promote pancreatic ductal adenocarcinoma progression through the miR-1294/c-Myc axis.

Mol Cancer 2021 08 21;20(1):106. Epub 2021 Aug 21.

Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, 270 Dong'An Road, Shanghai, 200032, PR China.

Background: Extensive studies have demonstrated the pivotal roles of circular RNAs (circRNAs) in the occurrence and development of different human cancers. However, the expression and regulatory roles of circRNAs in pancreatic ductal adenocarcinoma (PDAC) are unclear.

Methods: CircEYA3 was explored based on Gene Expression Omnibus (GEO) dataset analysis. qRT-PCR was applied to determine the expression of circRNAs, miRNAs and mRNAs in PDAC cells and tissues. The biological roles of circEYA3 in vitro and in vivo were determined by performing a series of functional experiments. Further, dual luciferase reporter, fluorescence in situ hybridization (FISH), RNA pull-down assays, and RNA immunoprecipitation (RIP) assays were used to confirm the interaction of circEYA3 with miR-1294.

Results: CircEYA3 was elevated in PDAC tissues and cells, and a higher level of circEYA3 was significantly associated with a poorer prognosis in patients with PDAC. Functionally, circEYA3 increased energy production via ATP synthesis to promote PDAC progression in vitro and in vivo. Mechanistically, circEYA3 functions as an endogenous miR-1294 sponge to elevate c-Myc expression, thus exerting its oncogenic functions.

Conclusion: CircEYA3 promotes the progression of PDAC through the miR-1294/c-Myc signalling axis, and circEYA3 may be an efficient molecular therapeutic target in PDAC.
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http://dx.doi.org/10.1186/s12943-021-01400-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379744PMC
August 2021

Study of aerosol characteristics and sources using MAX-DOAS measurement during haze at an urban site in the Fenwei Plain.

J Environ Sci (China) 2021 Sep 5;107:1-13. Epub 2021 Feb 5.

Institute of Atmospheric Physics, Chinese Academy of Science, Beijing 100029, China.

Atmospheric aerosols have effects on atmospheric radiation assessments, global climate change, local air quality and visibility. In particular, aerosols are more likely transformed and accumulated in winter. In this paper, we used the Multi-Axis Differential Optical Absorption Spectroscopy (MAX-DOAS) instrument to study the characteristics of aerosol type and contributions of PM chemical components to aerosol extinction (AE), vertical distribution of aerosols, and source. From December 30, 2018 to January 27, 2019, we conducted MAX-DOAS observations on Sanmenxia. The proportion of PM to PM was 69.48%-95.39%, indicating that the aerosol particles were mainly fine particles. By analyzing the ion data and modifying Interagency Monitoring of Protected Visual Environments (IMPROVE) method, we found that nitrate was the largest contributor to AE, accounting for 31.51%, 28.98%, and 27.95% of AE on heavily polluted, polluted, and clean days, respectively. NH, OC, and SO were also major contributors to AE. The near-surface aerosol extinction retrieved from MAX-DOAS measurement the PM and PM concentrations measured by an Unmanned Aerial Vehicle (UAV) have the same trend in vertical distribution. AE increased about 3 times from surface to 500 m. With the backward trajectory of the air mass during the haze, we also found that the continuous heavy pollution was mainly caused by transport of polluted air from the northeast, then followed by local industrial emissions and other sources of emissions under continuous and steady weather conditions.
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http://dx.doi.org/10.1016/j.jes.2020.12.015DOI Listing
September 2021

Chemical screening reveals Ronidazole is a superior pro-drug to Metronidazole for Nitroreductase-induced cell ablation system in zebrafish larvae.

J Genet Genomics 2021 Aug 16. Epub 2021 Aug 16.

Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology, Guangzhou, 510006, China. Electronic address:

The Metronidazole (MTZ)/nitroreductase (NTR)-mediated cell ablation system is the most commonly used chemical-genetic cell ablation method in zebrafish. This system can specifically ablate target cells under spatial and temporal control. The MTZ/NTR system has become a widely used cell ablation system in biological, developmental, and functional studies. However, the inadequate cell-ablation ability of some cell types and the side effects of high concentration MTZ impede extensive applications of the MTZ/NTR system. In the present study, the US drug collection library was searched to extend the NTR system. Six MTZ analogs were found and the cell-ablation ability of these analogs was tested in zebrafish larvae. The results revealed that two of the NTR substrates, Furazolidone and Ronidazole, ablated target cells more efficiently than MTZ at lower concentrations. Furthermore, the working concentration of Ronidazole, but not Furazolidone and MTZ, did not affect axonal bridge formation during spinal cord regeneration. Taken together, our results indicate that Ronidazole is a superior pro-drug to MTZ for the NTR system, especially for the study of neuron regeneration in zebrafish larvae.
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http://dx.doi.org/10.1016/j.jgg.2021.07.015DOI Listing
August 2021

The Value of Subjective Visual Vertical in Diagnosis of Vestibular Migraine.

Curr Med Sci 2021 Aug 17;41(4):654-660. Epub 2021 Aug 17.

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Objective: To study the value of the subjective visual vertical (SVV) in the diagnosis of vestibular migraine (VM).

Methods: This study recruited 128 VM patients and 64 age-matched normal subjects. We detected the SVV during the interval between attacks in both groups, in sitting upright, and the head tilted at 45° to the left or right. We then examined the correlation between the SVV results with the vestibular evoked myogenic potential (VEMP) and canal paresis (CP).

Results: It was found there was a significant difference in SVV at the upright position between VM patients and normal controls (P=0.006) and no significant difference was found at the tilts of 45° to the left or right between the two groups. The SVV results at the upright position were significantly correlated with cervical VEMP (P=0.042) whereas not significantly correlated with CP and VEMP. There existed no significant difference in the conformity to the Müller effect (M effect) between the two groups. ROC analysis exhibited that the sensitivity, specificity of SVVs at the upright were 67.200% and 62.500% respectively. The diagnostic value of SVV at the upright position was significantly higher than that at tilts of 45° to the left and right (P=0.006). Nonetheless the diagnostic accuracy was relatively low.

Conclusion: Abnormality in SVV possibly stems from the lasting functional disorder of cerebellar or high-level cortical centers in VM patients or is linked to the vestibular compensation. The SVV is of low diagnostic value for VM and the value of SVV in VM warrants further study.
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http://dx.doi.org/10.1007/s11596-021-2418-yDOI Listing
August 2021

Mechanism of Fei-Xian Formula in the Treatment of Pulmonary Fibrosis on the Basis of Network Pharmacology Analysis Combined with Molecular Docking Validation.

Evid Based Complement Alternat Med 2021 3;2021:6658395. Epub 2021 Aug 3.

Department of Respiratory Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, Jiangsu Province, China.

Objective: This study aimed to clarify the mechanism of Fei-Xian formula (FXF) in the treatment of pulmonary fibrosis based on network pharmacology analysis combined with molecular docking validation.

Methods: Firstly, ingredients in FXF with pharmacological activities, together with specific targets, were identified based on the BATMA-TCM and TCMSP databases. Then, targets associated with pulmonary fibrosis, which included pathogenic targets as well as those known therapeutic targets, were screened against the CTD, TTD, GeneCards, and DisGeNet databases. Later, Cytoscape was employed to construct a candidate component-target network of FXF for treating pulmonary fibrosis. In addition, for nodes within the as-constructed network, topological parameters were calculated using CytoHubba plug-in, and the degree value (twice as high as the median degree value for all the nodes) was adopted to select core components as well as core targets of FXF for treating pulmonary fibrosis, which were subsequently utilized for constructing the core network. Furthermore, molecular docking study was carried out on those core active ingredients together with the core targets using AutoDock Vina for verifying results of network pharmacology analysis. At last, OmicShare was employed for enrichment analysis of the core targets.

Results: Altogether 12 active ingredients along with 13 core targets were identified from our constructed core component-target network of FXF for the treatment of pulmonary fibrosis. As revealed by enrichment analysis, the 13 core targets mostly concentrated in regulating biological functions, like response to external stimulus (from oxidative stress, radiation, UV, chemical substances, and virus infection), apoptosis, cell cycle, aging, immune process, and protein metabolism. In addition, several pathways, like IL-17, AGE-RAGE, TNF, HIF-1, PI3K-AKT, NOD-like receptor, T/B cell receptor, and virus infection-related pathways, exerted vital parts in FXF in the treatment of pulmonary fibrosis.

Conclusions: FXF can treat pulmonary fibrosis through a "multicomponent, multitarget, and multipathway" mean. Findings in this work lay foundation for further exploration of the FXF mechanism in the treatment of pulmonary fibrosis.
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http://dx.doi.org/10.1155/2021/6658395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357467PMC
August 2021

Intranasal temperature-sensitive hydrogels of cannabidiol inclusion complex for the treatment of post-traumatic stress disorder.

Acta Pharm Sin B 2021 Jul 24;11(7):2031-2047. Epub 2021 Jan 24.

Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, China.

Post-traumatic stress disorder (PTSD) is a psychiatric disease that seriously affects brain function. Currently, selective serotonin reuptake inhibitors (SSRIs) are used to treat PTSD clinically but have decreased efficiency and increased side effects. In this study, nasal cannabidiol inclusion complex temperature-sensitive hydrogels (CBD TSGs) were prepared and evaluated to treat PTSD. Mice model of PTSD was established with conditional fear box. CBD TSGs could significantly improve the spontaneous behavior, exploratory spirit and alleviate tension in open field box, relieve anxiety and tension in elevated plus maze, and reduce the freezing time. Hematoxylin and eosin and c-FOS immunohistochemistry slides showed that the main injured brain areas in PTSD were the prefrontal cortex, amygdala, and hippocampus CA1. CBD TSGs could reduce the level of tumor necrosis factor- caused by PTSD. Western blot analysis showed that CBD TSGs increased the expression of the 5-HT1A receptor. Intranasal administration of CBD TSGs was more efficient and had more obvious brain targeting effects than oral administration, as evidenced by the pharmacokinetics and brain tissue distribution of CBD TSGs. Overall, nasal CBD TSGs are safe and effective and have controlled release. There are a novel promising option for the clinical treatment of PTSD.
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http://dx.doi.org/10.1016/j.apsb.2021.01.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343172PMC
July 2021

Comparative Analysis of the Chloroplast Genome for Four Species: Molecular Structure and Phylogenetic Relationships.

Front Genet 2021 27;12:687844. Epub 2021 Jul 27.

Institute of Plant Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, China.

The genus (Poaceae) is both a forage crop and staple food crop in the tropics. In this study, we obtained chloroplast genome sequences of four species of (, , , and ) using Illumina sequencing. These chloroplast genomes have circular structures of 136,346-138,119 bp, including a large single-copy region (LSC, 79,380-81,186 bp), a small single-copy region (SSC, 12,212-12,409 bp), and a pair of inverted repeat regions (IRs, 22,284-22,372 bp). The overall GC content of these chloroplast genomes was 38.6-38.7%. The complete chloroplast genomes contained 110 different genes, including 76 protein-coding genes, 30 transfer RNA (tRNA) genes, and four ribosomal RNA (rRNA) genes. Comparative analysis of nucleotide variability identified nine intergenic spacer regions (, and ), which may be used as potential DNA barcodes in future species identification and evolutionary analysis of . The phylogenetic analysis revealed a close relationship between and , followed by and . The completed genomes of this study will help facilitate future research on the phylogenetic relationships and evolution of species.
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http://dx.doi.org/10.3389/fgene.2021.687844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354216PMC
July 2021

Unusual Temperature Dependence of Bandgap in 2D Inorganic Lead-Halide Perovskite Nanoplatelets.

Adv Sci (Weinh) 2021 Aug 11:e2100084. Epub 2021 Aug 11.

CAS Key Laboratory of Design and Assembly of Functional Nanostructures, State Key Laboratory of Structural Chemistry, and Fujian Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, China.

Understanding the origin of temperature-dependent bandgap in inorganic lead-halide perovskites is essential and important for their applications in photovoltaics and optoelectronics. Herein, it is found that the temperature dependence of bandgap in CsPbBr perovskites is variable with material dimensionality. In contrast to the monotonous redshift ordinarily observed in bulk-like CsPbBr nanocrystals (NCs), the bandgap of 2D CsPbBr nanoplatelets (NPLs) exhibits an initial blueshift then redshift trend with decreasing temperature (290-10 K). The Bose-Einstein two-oscillator modeling manifests that the blueshift-redshift crossover of bandgap in the NPLs is attributed to the significantly larger weight of contribution from electron-optical phonon interaction to the bandgap renormalization in the NPLs than in the NCs. These new findings may gain deep insights into the origin of bandgap shift with temperature for both fundamentals and applications of perovskite semiconductor materials.
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http://dx.doi.org/10.1002/advs.202100084DOI Listing
August 2021

A "Null" Pattern of p16 Immunostaining in Endometrial Serous Carcinoma: An Under-recognized and Important Aberrant Staining Pattern.

Int J Gynecol Pathol 2021 Aug 11. Epub 2021 Aug 11.

Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health (D.R.M., V.L.H., W.M.R., P.W., J.X.) UW Health Clinical Laboratories, University of Wisconsin Hospitals and Clinics (M.A.A.) Wisconsin State Laboratory of Hygiene, (L.H., X.S., L.F.-R., V.L.H.), Madison, Wisconsin.

The ability to distinguish endometrial serous carcinoma (SC) from high-grade endometrioid adenocarcinoma is of great importance given their differences in prognosis and management. In practice, this distinction typically relies upon the use of a focused immunohistochemical panel including p53, p16, and mismatch repair proteins. The expression of p16 is characteristically strong and diffuse in SCs, and weak and/or patchy in many high-grade endometrioid adenocarcinomas. Here, we report a subset of SCs that are entirely negative for p16 immunostaining, a pattern we refer to as "p16 null." This pattern was identified in 2 of 63 cases of SC diagnosed at our institution-1 with histologically classic features and 1 with ambiguous high-grade histologic features. These tumors otherwise showed a SC signature by immunohistochemical and demonstrated an SC pattern of genetic mutations. No mutation in the gene for p16, cyclin-dependent kinase inhibitor 2A (CDKN2A), was identified in either case. However, molecular correlates for the absent p16 expression were present, including homozygous deletion of CDKN2A in one case and hemizygous deletion of CDKN2A with promotor hypermethylation of the remaining allele in the other case. To our knowledge, this constitutes the first report conclusively demonstrating the existence of a small subset of SCs that are completely negative by p16 immunohistochemistry, and the molecular lesions responsible for this pattern. In the context of an otherwise clinically and histologically classic example of SC, we endorse this "null" p16 staining pattern as an alternative aberrant staining pattern that should not deter one from committing to this diagnosis.
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http://dx.doi.org/10.1097/PGP.0000000000000817DOI Listing
August 2021

Clinical efficacy of mineralized collagen (MC) versus anorganic bovine bone (Bio-Oss) for immediate implant placement in esthetic area: a single-center retrospective study.

BMC Oral Health 2021 Aug 10;21(1):390. Epub 2021 Aug 10.

Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, Shandong, China.

Background: The purpose of this retrospective study was to evaluate the clinical efficacy of mineralized collagen (MC) versus anorganic bovine bone (Bio-Oss) for immediate implant placement in esthetic area.

Methods: Medical records of Department of Oral and Maxillofacial Surgery of Shandong Provincial Hospital were screened for patients who had been treated with immediate implant implantation in the esthetic area using either MC (Allgens®, Beijing Allgens Medical Science and Technology Co., Ltd., China) or Bio-Oss (Bio-Oss®, Geistlich Biomaterials, Wolhusen, Switzerland), between January 2018 and December 2019. All patients fulfilling the in-/exclusion criteria and following followed for a minimum period of 1 year after surgery were enrolled into the presented study. Implant survival rate, radiographic, esthetic and patient satisfactory evaluations were performed.

Results: Altogether, 70 patients were included in the study; a total of 80 implants were inserted. All implants had good initial stability. The survival rate of implants was 100% at 1-year follow-up. The differences in horizontal and vertical bone loss between the MC group (0.72 ± 0.26 mm, 1.62 ± 0.84 mm) and the Bio-Oss group (0.70 ± 0.52 mm, 1.57 ± 0.88 mm) were no significant difference statistically no significant 6 months after permanent restoration. Similar results occurred at 12 months after permanent restoration functional loaded. Clinical acceptability defined by pink esthetic score (PES) ≥ 6 (6.07 ± 1.62 vs. 6.13 ± 1.41) was not significantly different between groups. Patient satisfaction estimated by visual analog scale (VAS) was similar (8.56 ± 1.12 vs. 8.27 ± 1.44), and the difference was no significant difference between the two groups.

Conclusions: The biomimetic MC showed a similar behaviour as Bio-Oss not only in its dimensional tissues changes but also in clinical acceptability and patient satisfaction. Within the limitations of this study, these cases show that MC could be considered as an alternative bone graft in IIP.
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http://dx.doi.org/10.1186/s12903-021-01752-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353785PMC
August 2021

Genetic and epigenetic orchestration of Gfi1aa-Lsd1-cebpa in zebrafish neutrophil development.

Development 2021 Sep 3;148(17). Epub 2021 Sep 3.

Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology, Guangzhou 510006, People's Republic of China.

Neutrophils are the most abundant vertebrate leukocytes and they are essential to host defense. Despite extensive investigation, the molecular network controlling neutrophil differentiation remains incompletely understood. GFI1 is associated with several myeloid disorders, but its role and the role of its co-regulators in granulopoiesis and pathogenesis are far from clear. Here, we demonstrate that zebrafish gfi1aa deficiency induces excessive neutrophil progenitor proliferation, accumulation of immature neutrophils from the embryonic stage, and some phenotypes similar to myelodysplasia syndrome in adulthood. Both genetic and epigenetic analyses demonstrate that immature neutrophil accumulation in gfi1aa-deficient mutants is due to upregulation of cebpa transcription. Increased transcription was associated with Lsd1-altered H3K4 methylation of the cebpa regulatory region. Taken together, our results demonstrate that Gfi1aa, Lsd1 and cebpa form a regulatory network that controls neutrophil development, providing a disease progression-traceable model for myelodysplasia syndrome. Use of this model could provide new insights into the molecular mechanisms underlying GFI1-related myeloid disorders as well as a means by which to develop targeted therapeutic approaches for treatment.
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http://dx.doi.org/10.1242/dev.199516DOI Listing
September 2021

Analysis of Immune-Related Signatures Related to CD4+ T Cell Infiltration With Gene Co-Expression Network in Pancreatic Adenocarcinoma.

Front Oncol 2021 23;11:674897. Epub 2021 Jul 23.

Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.

Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the most invasive solid malignancies. Immunotherapy and targeted therapy confirmed an existing certain curative effect in treating PDAC. The aim of this study was to develop an immune-related molecular marker to enhance the ability to predict Stages III and IV PDAC patients.

Method: In this study, weighted gene co-expression network (WGCNA) analysis and a deconvolution algorithm (CIBERSORT) that evaluated the cellular constituent of immune cells were used to evaluate PDAC expression data from the GEO (Gene Expression Omnibus) datasets, and identify modules related to CD4+ T cells. LASSO Cox regression analysis and Kaplan-Meier curve were applied to select and build prognostic multi-gene signature in TCGA Stages III and IV PDAC patients (N = 126). This was followed by independent Stages III and IV validation of the gene signature in the International Cancer Genome Consortium (ICGC, N = 62) and the Fudan University Shanghai Cancer Center (FUSCC, N = 42) cohort. Inherited germline mutations and tumor immunity exploration were applied to elucidate the molecular mechanisms in PDAC. Univariate and Multivariate Cox regression analyses were applied to verify the independent prognostic factors. Finally, a prognostic nomogram was created according to the TCGA-PDAC dataset.

Results: A four-gene signature comprising NAPSB, ZNF831, CXCL9 and PYHIN1 was established to predict overall survival of PDAC. This signature also robustly predicted survival in two independent validation cohorts. The four-gene signature could divide patients into high and low-risk groups with disparity overall survival verified by a Log-rank test. Expression of four genes positively correlated with immunosuppression activity (PD-L1 and PD1). Immune-related genes nomogram and corresponding calibration curves showed significant performance for predicting 3-year survival in TCGA-PDAC dataset.

Conclusion: We constructed a novel four-gene signature to predict the prognosis of Stages III and IV PDAC patients by applying WGCNA and CIBERSORT algorithm scoring to transcriptome data different from traditional methods of filtrating for differential genes in cancer and healthy tissues. The findings may provide reference to predict survival and was beneficial to individualized management for advanced PDAC patients.
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http://dx.doi.org/10.3389/fonc.2021.674897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343184PMC
July 2021
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