Publications by authors named "Xu Hu"

375 Publications

A Random-Screening Approach to Identify RNAi Targets for the Control of Western Corn Rootworm (Diabrotica. virgifera virgifera Le Conte).

Authors:
Xu Hu Adane Kassa

Methods Mol Biol 2022 ;2360:91-103

Corteva AgriScience, Johnston, IA, USA.

Identification of active target genes in bioassay screening is the first important step for application of RNA interference (RNAi) for pest control. Here, we describe the methodology for performing high-throughput RNAi target screening against important agriculture pest, Western corn rootworm in 96-well microplate. Two approaches are presented to identify active targets from random-cDNA library or testing a certain group of specific targets via in silico sequence analysis. Methods of PCR primer design, DNA template preparation, and dsRNA production described here can be applied for other pests.
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http://dx.doi.org/10.1007/978-1-0716-1633-8_9DOI Listing
January 2022

Visualization of RNA Transcripts in Western Corn Rootworm (Diabrotica virgifera virgifera) and Plants by In Situ Hybridization.

Methods Mol Biol 2022 ;2360:59-74

Corteva Agriscience, Johnston, IA, USA.

In situ hybridization (ISH) is a methodology by which nucleic acids are detected within fixed tissue samples. Recent advances in detection technology and target recovery have greatly enhanced the technique's ability to detect single mRNA molecules. Here we detail the fixation, paraffin embedding, sectioning, target recovery, and chromogenic detection of an mRNA (DvSSJ1), encoding for a membrane protein associated with the smooth septate junction (SSJ) in Western corn rootworm [Diabrotica virgifera (Dv)]. Further, we demonstrate, the expression of dsRNA of DvSSJ1 in maize root tissues using signal amplification and background suppression technology.
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http://dx.doi.org/10.1007/978-1-0716-1633-8_6DOI Listing
January 2022

Single Iridium Atom Doped NiP Catalyst for Optimal Oxygen Evolution.

J Am Chem Soc 2021 Sep 16;143(34):13605-13615. Epub 2021 Aug 16.

Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

Single-atom catalysts (SACs) with 100% active sites have excellent prospects for application in the oxygen evolution reaction (OER). However, further enhancement of the catalytic activity for OER is quite challenging, particularly for the development of stable SACs with overpotentials <180 mV. Here, we report an iridium single atom on NiP catalyst (Ir-NiP) with a record low overpotential of 149 mV at a current density of 10 mA·cm in 1.0 M KOH. The Ir-NiP catalyst delivers a current density up to ∼28-fold higher than that of the widely used IrO at 1.53 V vs RHE. Both the experimental results and computational simulations indicate that Ir single atoms preferentially occupy Ni sites on the top surface. The reconstructed Ir-O-P/Ni-O-P bonding environment plays a vital role for optimal adsorption and desorption of the OER intermediate species, which leads to marked enhancement of the OER activity. Additionally, the dynamic "top-down" evolution of the specific structure of the [email protected] particles is responsible for the robust single-atom structure and, thus, the stability property. This Ir-NiP catalyst offers novel prospects for simplifying decoration strategies and further enhancing OER performance.
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http://dx.doi.org/10.1021/jacs.1c04682DOI Listing
September 2021

Anchoring an Fe Dimer on Nitrogen-Doped Graphene toward Highly Efficient Electrocatalytic Ammonia Synthesis.

ACS Appl Mater Interfaces 2021 Sep 30;13(36):43632-43640. Epub 2021 Aug 30.

Department of Physics, Southern University of Science and Technology, Shenzhen 518055, China.

Electrochemical reduction of N to NH based on sustainable energy is a green technique to produce decentralized and on-demand ammonia. In this work, taking graphene as a design platform, we explore the dual-atom catalysts (DACs) via embedding two homonuclear transition metal (TM) atoms into graphene decorated with four neighboring pyrrolic nitrogen atoms ([email protected]) to computationally screen the qualified nitrogen reduction reaction (NRR) catalysts. On the basis of the activity, selectivity, and stability of 15 homonuclear DACs of [email protected], [email protected] is identified as the most efficient NRR catalyst with a limiting potential of only -0.32 V. Electronic structure analysis demonstrates that the low oxidation state of Fe (+1) remarkably activates the molecular N, which contributes to its excellent NRR catalytic activity. Moreover, the kinetic studies reveal all of the NRR elementary steps exhibiting barriers smaller than that of the hydrogen evolution reaction (HER), showing that HER is effectively suppressed. In addition, we find that the integral crystal orbital Hamilton population (ICOHP) can be used as a descriptor to describe the Gibbs free energy of each step for its NRR performance. This work not only provides theoretical guidance for designing DACs for NRR but also promotes the understanding of DACs for N fixation.
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http://dx.doi.org/10.1021/acsami.1c11585DOI Listing
September 2021

Anodized graphite felt as an efficient cathode for in-situ hydrogen peroxide production and Electro-Fenton degradation of rhodamine B.

Chemosphere 2021 Aug 18;286(Pt 3):131936. Epub 2021 Aug 18.

School of Materials Science and Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, Gansu, China. Electronic address:

This work investigated that the graphite felt anodized by NaOH, NHHCO, or HSO aqueous, and then as the cathode materials for in-situ hydrogen peroxide (HO) production and its employed for rhodamine B (RhB) degradation via Electro-Fenton (EF) process. At -0.60 V (vs. SCE), after 120 min electrolysis, the HO yield by graphite felt which anodized by 0.2 M HSO achieved up 110.5 mg L in 0.05 M NaSO electrolyte. Compared with the raw graphite felt used for cathode, the HO yield increased by 15.85 times under the same conditions. The results of Raman spectroscopy demonstrated that graphite felt anodized by HSO solution can be achieved the highest defect degree. For the degradation of RhB, the cathode which anodized by HSO solution has the highest removal rate. For the degradation rate of RhB, the effect of applied current density, Fe ions concentration, pH value were investigated. In addition, suggested that the efficient Fe reduction reaction on the cathode surface was an important reason of the high efficiency of RhB degradation. 5-times continuous runs indicated that the modified cathode has remarkable stability and reusability during the EF process.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131936DOI Listing
August 2021

[Overexpression of human EP4 receptor in vascular smooth muscle cells attenuates angiotensin II-induced hypertension in mice].

Sheng Li Xue Bao 2021 Aug;73(4):597-605

Health Science Center, East China Normal University, Shanghai 200241, China.

Prostaglandin E (PGE) plays an important role in cardiovascular system. PGE regulates blood pressure through its 4 G protein coupled receptors, i.e., EP1, EP2, EP3, and EP4. The aim of this study was to investigate the role of EP4 receptors in vascular smooth muscle cells (VSMC) in blood pressure regulation. VSMC-specific human EP4 transgenic (VSMC-hEP4 Tg) mice were generated and genotyped. The systolic blood pressure (SBP) of the VSMC-hEP4 Tg mice and the wild-type (WT) littermates was measured under normal, low-salt (LSD) and high-salt diet (HSD) conditions using a tail-cuff method. Both WT and VSMC-hEP4 Tg mice were administered with a chronic infusion of angiotensin II (Ang II) with an osmotic pump and SBP levels were monitored every week. The mean arterial blood pressure (MAP) of WT and VSMC-hEP4 Tg mice upon Ang II intravenous infusion was measured via carotid arterial catheterization. Ang II-induced vasoconstriction of the mesenteric arterial rings from WT and VSMC-hEP4 Tg mice was measured using the multi myograph system. The effect of PGE-OH (a selective EP4 agonist) on Ang II-induced phosphorylation of myosin phosphatase target subunit 1 (MYPT1) was detected by Western blot. The effect of two additional EP4 specific agonists (CAY10580 and CAY10598, 0.5 mg/kg) on blood pressure of WT mice was measured by carotid arterial catheterization. The results showed that the VSMC-hEP4 Tg mice were successfully generated and their basal SBP levels were lower than those of WT mice. Although blood pressure levels were significantly altered in WT mice under LSD and HSD, little change was observed in the VSMC-hEP4 Tg mice. After a chronic infusion and an acute intravenous injection of Ang II, SBP levels of VSMC-hEP4 Tg mice were significantly lower than those of WT mice. In addition, both CAY10580 and CAY10598 significantly reduced MAP levels of WT mice. Ex vivo study showed that treatment of isolated mesenteric arteries with PGE-OH inhibited Ang II-induced phosphorylation of MYPT1. Collectively, these results demonstrate that specific overexpression of human EP4 gene in VSMCs significantly reduces basal blood pressure levels and attenuates Ang II-induced hypertension, possibly via inhibiting Ang II/AT1 signaling pathway. Our findings suggest that EP4 may represent an attractive target for the treatment of hypertension.
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August 2021

Stability and catalytic performance of single-atom supported on Ti2CO2 for low-temperature CO oxidation: A first-principles study.

Chemphyschem 2021 Aug 14. Epub 2021 Aug 14.

Southern University of Science and Technology, Physics, 1088 Xueyuan Avenue, 518055, Shenzhen, CHINA.

Based on first-principles calculations, the potential of Ti2CO2 monolayer (MXene) as a single-atom catalyst (SAC) support for 3d transition metal (TM) atoms (Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, and Zn) is studied for CO oxidation. We first screen the support effect according to the stability of a single metal atom and find that Sc and Ti supported on Ti2CO2 have stronger adsorption energy than the cohesive energy of their bulk counterparts, therefore we selected Sc and Ti supported on Ti2CO2 for further catalytic reactions. The stability and the potential catalytic reactivity are verified by electronic structure and charge transfer analysis. Both Eley-Rideal (E-R) and Langmuir-Hinshelwood (LH) mechanisms are taken into account in this study, and lower energy barriers of 0.002 and 0.37 eV were found in the ER mechanism compared to the LH mechanism which are 0.25 and 0.34 eV for Sc and Ti catalysts, respectively. Moreover, kinetic ER and LH mechanisms are favorable for both Sc- and Ti/Ti2CO2 because of the comparable energy barrier to other metals and SAC supported on 2D materials. However, it is thermodynamically unfavorable for Ti/Ti2CO2 catalyst. Based on these calculations, we propose that Sc supported on Ti2CO2 is the best catalyst to catalyze CO-oxidation. The current study not only broadens the scope of the single-atom Sc catalyst but also extends the consideration of MXene support for catalyst optimization.
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http://dx.doi.org/10.1002/cphc.202100436DOI Listing
August 2021

Design and synthesis of β-carboline derivatives with nitrogen mustard moieties against breast cancer.

Bioorg Med Chem 2021 Sep 2;45:116341. Epub 2021 Aug 2.

Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China. Electronic address:

To discover the promising antitumor agents, a series of β-carboline derivatives with nitrogen mustard moieties were designed and synthesized. Most target derivatives showed antiproliferative activity against MCF-7 and MDA-MB-231 cells. Among them, (1-methyl-9H-pyrido[3,4-b]indol-3-yl)methyl (S)-3-(4-(bis(2-chloroethyl)amino)phenyl)-2-formamidopropanoate possessed the most potent antiproliferative activity with IC values of 1.79 μM and 4.96 μM, respectively, which were significantly higher than that of the parent compounds, and the efficacy was comparable to that of the positive control doxorubicin. More importantly, it showed weak cytotoxicity against human normal breast cell line MCF-10A (IC > 20 μM), exhibiting certain selectivity. Subsequently, further mechanism exploration indicated that it induced G2/M phase cell cycle arrest and apoptosis in MDA-MB-231 cells. The DCFH-DA fluorescent probe assay and comet assay showed that this compound could cause intracellular ROS accumulation and DNA damage. In addition, it exerted potent inhibitory effect on the migration, invasion and adhesion of MDA-MB-231 cells in vitro. In short, (1-methyl-9H-pyrido[3,4-b]indol-3-yl)methyl (S)-3-(4-(bis(2-chloroethyl)amino)phenyl)-2-formamidopropanoate was considered as a promising compound for anti-breast cancer.
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http://dx.doi.org/10.1016/j.bmc.2021.116341DOI Listing
September 2021

Index-based dietary patterns and stomach cancer in a Chinese population.

Eur J Cancer Prev 2021 07 20. Epub 2021 Jul 20.

Department of Epidemiology, Fielding School of Public Health, University of California at Los Angeles (UCLA), Los Angeles, California, USA Department of Non-communicable Chronic Disease Control, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu Province Department of Epidemiology, School of Public Health, Fudan University, Shanghai Ganyu County Center for Disease Control and Prevention, Ganyu Dafeng County Center for Disease Control and Prevention, Dafeng Chuzhou County Center for Disease Control and Prevention, Chuzhou Tongshan County Center for Disease Control and Prevention, Tongshan, Jiangsu Province, China Jonsson Comprehensive Cancer Center, UCLA School of Nursing, UCLA, Los Angeles, California, USA Department of Epidemiology and Biostatistics, Peking University School of Public Health, Beijing, China Department of Social and Preventive Medicine, State University of New York at Buffalo, Buffalo, New York Center for Human Nutrition, Department of Medicine, UCLA David Geffen School of Medicine, University of California at Los Angeles (UCLA), Los Angeles, California, USA Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.

Objectives: Dietary factors are of importance in the development of stomach cancer. This study aims to examine index-based dietary patterns associated with stomach cancer in a Chinese population.

Methods: Using data from a population-based case-control study conducted in Jiangsu Province, China, we included a total of 8432 participants (1900 stomach cancer cases and 6532 controls). Dietary data collected by food frequency questionnaire was evaluated by modified Chinese Healthy Eating Index-2016 (mCHEI-2016) and the US Healthy Eating Index-2015 (HEI-2015). Multiple logistic regression analyses were applied to examine the association of mCHEI-2016 and HEI-2015 with stomach cancer while adjusting for potential confounders. The possible interactions between mCHEI-2016 or HEI-2015 and established risk factors were explored.

Results: Among nonproxy interviews, after adjusting for potential confounding factors, a higher score of sodium, reflecting lower intake per day, was inversely associated with stomach cancer [odds ratio (OR), 0.95; 95% CI, 0.91-0.99 for mCHEI-2016; OR, 0.97; 95% CI, 0.94-0.99 for HEI-2015]. No clear associations with stomach cancer were identified for total scores of HEI-2015 (OR, 0.98; 95% CI, 0.87-1.10 with a 10-point increase, P trend = 0.98) and mCHEI-2016 (OR, 1.05; 95% CI, 0.94-1.17 with a 10-point increase, P trend = 0.22). However, the relation between stomach cancer and the mCHEI-2016 was modified by BMI, with a possible inverse association in normal-weight subjects.

Conclusions: Our findings highlight that reduced intake of dietary sodium would prevent the development of stomach cancer. The data indicate a heterogeneity between normal weight and overweight's dietary factors in relation to stomach cancer.
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http://dx.doi.org/10.1097/CEJ.0000000000000705DOI Listing
July 2021

Prognostic Values of Different Clinicopathological Factors and Predictive Models for Penile Carcinoma.

Cancer Manag Res 2021 12;13:5623-5632. Epub 2021 Jul 12.

Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China.

Objective: To evaluate the prognostic factors of penile cancer and the utility of prognostic models.

Methods: We analyzed postoperatively collected data of 311 patients diagnosed with penile cancer. Survival analysis (Kaplan-Meier and cox regression methods) was performed on this cohort. The c-index was used to determine the predictive accuracies of potential prognostic factors. The accuracies of four prognostic models were also evaluated, which were AJCC prognostic stage group for three recent editions, and four nomograms constructed by the Surveillance, Epidemiology, and End Results program (SEER). Two novel nomograms using our data were created and AUC of 2-year survival were determined to compare existing and newly established models.

Results: Tumor site, T and N stages, nuclear grade and lymph vascular invasion (LVI) significantly influenced prognosis. The 8th T and N stages had better c-indexes than former editions, while no improvement was seen in the 8thAJCC stage group. 6th AJCC+grade nomogram had a higher c-index than other three nomograms (SEER+grade, 6th TNM+grade, and 6th TN+grade nomograms; c-index: 0.831 vs 0.738, 0.792 and 0.781). New nomogram 1 included the 8th T and N stages, tumor site, nuclear grade, and LVI, with a c-index of 0.870. Novel nomogram 2 replaced the T and N stages with the AJCC stage group, which had a lower c-index of 0.855. The order of prediction accuracy of 2-year survival in the old and new models is consistent with the c-index results.

Conclusion: Tumor site, stages, grade, and LVI play important roles in predicting survival of penile cancer. The 8th stages have better predictive accuracy than former editions. We proposed two models with better predictive accuracy than former models; specifically, nomogram 1 may be a more precise and convenient tool for predicting penile cancer outcomes.
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http://dx.doi.org/10.2147/CMAR.S323321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285520PMC
July 2021

Identification of electronic descriptors for catalytic activity of transition-metal and non-metal doped MoS.

Phys Chem Chem Phys 2021 Jul;23(28):15101-15106

Department of Physics & Shenzhen Institute for Quantum Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China. and Guangdong Provincial Key Laboratory of Computational Science and Material Design, Southern University of Science and Technology, Shenzhen, 518055, China and Guangdong-Hong Kong-Macao Joint Laboratory for Photonic-Thermal-Electrical Energy Materials and Devices, Southern University of Science and Technology, Shenzhen 518055, China.

While the d-band theory offers successful electronic descriptors for catalytic activity of transition metals, transition metal compounds still need substantial theoretical input for the identification of reactivity descriptors for fast screening of earth-abundant catalysts. We study transition metal (TM) and non-metal doped MoS2, a promising substitute for noble metals as catalysts for multiple reactions, to clarify how doping modifies the reactivity by regulating the electronic structure of the host. We find that doping can significantly change the density of states (DOS) at band edges and the position of the Fermi level, which renders the S p-band center εp a good descriptor for H adsorption on both TM and non-metal doped MoS2. Dopants to the left of the host elements in the periodic table that have fewer electrons pin the Fermi level into the valence band, and those to the right that have more electrons pin the Fermi level into the conduction band, resulting in separated linear relationships between H binding energy and S p-band center. Moreover, by a close examination of the electronic structure of late TM doped systems, we identify the position of the late TM dopant induced DOS peak near the conduction band minimum (CBM), εTM, as a refined descriptor which shows a linear relationship for H as well as C, N, O adsorption. Finally, we generalize our descriptor to MoSe2 and MoTe2 to include all three anions in transition metal dichalcogenides (TMDs) and show a universal scaling relationship between the H binding energy and anion p-band center. Together we further enhance our understanding on identifying electronic descriptors for TM compounds.
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http://dx.doi.org/10.1039/d1cp01458gDOI Listing
July 2021

Effects of sodium-glucose cotransporter 2 inhibitors on cardiovascular outcomes in elderly patients with comorbid coronary heart disease and diabetes mellitus.

J Geriatr Cardiol 2021 Jun;18(6):440-448

Chinese PLA Medical School, Chinese PLA General Hospital, Beijing, China.

Objective: To investigate the effects of sodium-glucose cotransporter 2 inhibitors (SGLT2i) on cardiovascular outcomes in elderly Chinese patients with comorbid coronary heart disease (CHD) and type 2 diabetes mellitus (T2DM).

Methods: A retrospective cohort study was conducted on 501 elderly inpatients (≥ 60 years) with comorbid CHD/T2DM in Department of Cardiovascular Medicine and Endocrinology, Chinese PLA General Hospital from January 2018 to December 2019. These patients were divided into two groups according to the administration of SGLT2i. All the demographic characteristics and clinical data were collected. Cardiovascular outcomes, including all-cause mortality, major adverse cardiovascular events (MACE), and hospitalization for heart failure (HHF), were followed up.

Results: In the cohort, there were 167 patients in the SGLT2i group and 334 patients in the control group. In the efficacy analyses, the incidence of MACE was lower in the SGLT2i group than in the control group: 3.6% . 9.3% ( = 0.022). A lower risk of MACE was observed in the SGLT2i group [hazard ratio (HR) = 0.40, 95% CI: 0.17-0.95]. There was no significant difference in the incidence of all-cause mortality or HHF between the two groups. No significant difference of HR was observed for all-cause mortality (HR = 0.41, 95% CI: 0.12-1.41) or HHF (HR = 0.58, 95% CI: 0.12-2.81).

Conclusions: SGLT2i treatment exhibited benefits for elderly patients with comorbid CHD/T2DM with a lower risk for MACE.
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http://dx.doi.org/10.11909/j.issn.1671-5411.2021.06.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220386PMC
June 2021

Nanoengineering of a newly designed chlorin e6 derivative for amplified photodynamic therapy regulating lactate metabolism.

Nanoscale 2021 Jul;13(27):11953-11962

Department of Pharmaceutics, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China.

Chlorin e6 (Ce6) is a widely utilized photosensitizer in photodynamic therapy (PDT) against tumor growth, but its hydrophobic feature and the hypoxia in the tumor microenvironment greatly compromise its therapeutic efficacy. To address the issues, here we designed a new Ce6 derivative (TCe6) by coupling Ce6 with amphiphilic d-α-tocopherol polyethylene glycol 1000 succinate (TPGS), endowing Ce6 with an excellent amphiphilic feature. In particular, the overall reactive oxygen species (ROS) generation by TCe6 was significantly enhanced because TPGS could interact with mitochondrial complex II to induce extra ROS production, amplifying the total ROS production under PDT. Inspired by the unique property of α-cyano-4-hydroxycinnamate (CHC) in regulating lactate metabolism to spare more intracellular oxygen for PDT, TCe6 was further co-assembled with CHC to construct TCe6/CHC nanoparticles (NPs) for addressing the insufficient oxygen issue in PDT. The as-prepared TCe6/CHC NPs not only increased the efficiency of cell internalization but also improved the solubility and stability of Ce6 and CHC. Thanks to the extra ROS production by the TPGS unit, the amphiphilic feature of TCe6 and the CHC-mediated hypoxia microenvironment, the TCe6/CHC NPs demonstrated excellent PDT against tumor growth. This work provided a versatile strategy to solve the current bottleneck in photosensitizer-based PDT, holding great promise for the design of advanced photodynamic nanoplatforms.
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http://dx.doi.org/10.1039/d1nr01083bDOI Listing
July 2021

Effectiveness and safety of tibial nerve stimulation versus anticholinergic drugs for the treatment of overactive bladder syndrome: a meta-analysis.

Ann Palliat Med 2021 Jun 9;10(6):6287-6296. Epub 2021 Jun 9.

Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.

Background: This meta-analysis aimed to evaluate the efficacy and safety of tibial nerve stimulation (TNS) and anticholinergic drugs (ACDs) for the treatment of overactive bladder syndrome (OAB).

Methods: Qualified studies up to November 3, 2020 were retrieved from PubMed, Embase, the Cochrane Library and Web of Science. Pooled mean differences (MDs) were calculated to evaluate the improvement of micturition frequency per day, nocturia per night, urinary urgency episodes per day, incontinence episodes per day, and voided volume each time. Odd ratios (ORs) were used to assess the discontinuation rate.

Results: A total of 6 RCTs including 291 patients were included. Comparing with those receiving ACDs, patients receiving TNS had a significantly better improvement of urge incontinence episodes (MD =-1.11; 95% CI: -1.66, -0.55; P<0.001), but had comparable effect on micturition (MD =-0.75; 95% CI: -1.78, 0.28; P=0.16), nocturia (MD =-1.11; 95% CI: -2.93, 0.72; P=0.23), urgency (MD =-0.57; 95% CI: -1.93, 0.79; P=0.41) and voided volume (MD =11.61; 95% CI: -3.95, 27.18; P=0.14). Moreover, TNS group had a significantly lower discontinuation rate (OR =0.13; 95% CI: 0.03, 0.51; P=0.003) during the trials.

Discussion: TNS is as effective as ACDs for treating OAB, but with better performance on the decrease of urge incontinence episodes. Besides, TNS appears to be more tolerable than ACDs.
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http://dx.doi.org/10.21037/apm-21-339DOI Listing
June 2021

New possible silver lining for pancreatic cancer therapy: Hydrogen sulfide and its donors.

Acta Pharm Sin B 2021 May 31;11(5):1148-1157. Epub 2020 Oct 31.

Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.

As one of the most lethal diseases, pancreatic cancer shows a dismal overall prognosis and high resistance to most treatment modalities. Furthermore, pancreatic cancer escapes early detection during the curable period because early symptoms rarely emerge and specific markers for this disease have not been found. Although combinations of new drugs, multimodal therapies, and adjuvants prolong survival, most patients still relapse after surgery and eventually die. Consequently, the search for more effective treatments for pancreatic cancer is highly relevant and justified. As a newly re-discovered mediator of gasotransmission, hydrogen sulfide (HS) undertakes essential functions, encompassing various signaling complexes that occupy key processes in human biology. Accumulating evidence indicates that HS exhibits bimodal modulation of cancer development. Thus, endogenous or low levels of exogenous HS are thought to promote cancer, whereas high doses of exogenous HS suppress tumor proliferation. Similarly, inhibition of endogenous HS production also suppresses tumor proliferation. Accordingly, HS biosynthesis inhibitors and HS supplementation (HS donors) are two distinct strategies for the treatment of cancer. Unfortunately, modulation of endogenous HS on pancreatic cancer has not been studied so far. However, HS donors and their derivatives have been extensively studied as potential therapeutic agents for pancreatic cancer therapy by inhibiting cell proliferation, inducing apoptosis, arresting cell cycle, and suppressing invasion and migration through exploiting multiple signaling pathways. As far as we know, there is no review of the effects of HS donors on pancreatic cancer. Based on these concerns, the therapeutic effects of some HS donors and NO-HS dual donors on pancreatic cancer were summarized in this paper. Exogenous HS donors may be promising compounds for pancreatic cancer treatment.
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http://dx.doi.org/10.1016/j.apsb.2020.10.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144891PMC
May 2021

Prognostic Significance of Prognostic Nutritional Index in Patients with Renal Cell Carcinoma: A Meta-Analysis.

Nutr Cancer 2021 Jun 1:1-9. Epub 2021 Jun 1.

Department of Urology, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China.

The prognostic nutrition index (PNI), based on the serum lymphocyte counts and albumin levels, has been introduced as a prognostic factor in various cancer. In the present study, we explore the prognostic significance of PNI in patients with renal cell carcinoma (RCC). A literature search of all publications was conducted using the Cochrane library, PubMed and Embase databases from inception to April 2020. A total of 12 studies consisting of 7,391 patients were enrolled in the present study. We found that low pretreatment PNI is significantly correlated to poor survival, including overall survival (OS) ( < 0.001), cancer-specific survival (CSS) ( = 0.002), progression-free survival/recurrence-free survival/disease-free survival (PFS/RFS/DFS) ( < 0.001). The age ( < 0.001), clear cell histology ( = 0.044), T3-T4 ( = 0.049), and Fuhrman grade 3-4 ( = 0.024) were significantly differed in the low and high pretreatment PNI group. In summary, low pretreatment PNI was associated with adverse clinicopathological features in patients with RCC. Besides, low pretreatment PNI was also an unfavorable factor of OS, CSS, and PFS/RFS/DFS in RCC patients, which could serve as an unfavorable factor. More studies with large participants are required to verify our results.Supplemental data for this article is available online at https://doi.org/10.1080/01635581.2021.1931702.
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http://dx.doi.org/10.1080/01635581.2021.1931702DOI Listing
June 2021

Interference of commissural connections through the genu of the corpus callosum specifically impairs sensorimotor gating.

Behav Brain Res 2021 Aug 25;411:113383. Epub 2021 May 25.

Key Lab of Organ Development and Regeneration of Zhejiang Province, Institute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 311121, China. Electronic address:

White matter abnormalities in schizophrenic patients are characterized as regional tract-specific. Myelin loss at the genu of the corpus callosum (GCC) is one of the most consistent findings in schizophrenic patients across the different populations. We characterized the axons that pass through the GCC by stereotactically injecting an anterograde axonal tracing viral vector into the forceps minor of the corpus callosum in one hemisphere, and identified the homotopic brain structures that have commissural connections in the two hemispheres of the prefrontal cortex, including the anterior cingulate area, the prelimbic area, the secondary motor area, and the dorsal part of the agranular insular area, along with commissural connections with the primary motor area, caudoputamen, and claustrum. To investigate whether dysmyelination in these commissural connections is critical for the development of schizophrenia symptoms, we generated a mouse model with focal demyelination at the GCC by stereotactically injecting demyelinating agent lysolecithin into this site, and tested these mice in a battery of behavioral tasks that are used to model the schizophrenia-like symptom domains. We found that demyelination at the GCC influenced neither the social interest or mood state, nor the locomotive activity or motor coordination. Nevertheless, it specifically reduced the prepulse inhibition of acoustic startle that is a well-known measure of sensorimotor gating. This study advances our understanding of the pathophysiological contributions of the GCC-specific white matter lesion to the related disease, and demonstrates an indispensable role of interhemispheric communication between the frontal cortices for the top-down regulation of the sensorimotor gating.
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http://dx.doi.org/10.1016/j.bbr.2021.113383DOI Listing
August 2021

Efficacy and safety of short-wave diathermy treatment for moderate COVID-19 patients: a prospective, double-blind, randomized controlled clinical study.

Eur J Phys Rehabil Med 2021 May 27. Epub 2021 May 27.

Department of Rehabilitation Medicine, Xijing Hospital, the Fourth Military Medical University, Xi'an, China -

Background: Millions of human beings have suffered in the epidemic of coronavirus disease 2019 (COVID-19), but until now the effective treatment methods have been limited.

Aim: This study aimed to evaluate the efficacy and safety of short-wave diathermy (SWD) treatment for moderate COVID-19 patients.

Design: A prospective, double-blind, randomized controlled clinical study.

Setting: Inpatients unit of a COVID-19 specialized hospital.

Population: Forty-two patients with moderate COVID-19 were randomly allocated at a 2:1 ratio to two groups: the SWD group and the control group.

Methods: Participants of the SWD group received SWD treatment, and participants of the control group received placebo SWD treatment for one session per day, 10 minutes per session, for no more than 14 days. Both groups were given standard care treatment. Primary outcome was the rate of clinical improvement according to a sevencategory ordinal scale. Secondary outcomes included the rate of computed tomography (CT) improvement and the rate of potential adverse events.

Results: Clinical improvement occurred in 92.6% of patients in the SWD group by day 14 compared with 69.2% of patients in the control group (P = 0.001). The Cox model indicated that the SWD group had a higher clinical improvement probability than the control group (Hazard Ratio, 3.045; 95% CI, 1.391-6.666; P = 0.005). Similarly, CT improvement occurred in 85.2 % of patients in the SWD group and 46.2 % of patients in the control group respectively by day 14 (P = 0.001). The Cox model indicated SWD group had a higher CT improvement probability than control group (Hazard Ratio, 3.720; 95% CI, 1.486-9.311; P = 0.005). There was no significant difference in adverse events between the SWD group and the control group (2 of 27 [7.4%] SWD vs. 1 of 13 [7.7%] control, P = 1.000), the most frequent of which were headache (1 of 27 [3.7%] SWD vs. 1 of 13 [7.7%] control patients) and dizziness (1 of 27 [3.7%] SWD vs. 0 of 13 [0%] control patients).

Conclusions: SWD is a valid and reliable adjuvant therapy with a favorable safety profile for moderate COVID-19 patients.

Clinical Rehabilitation Impact: Clinically relevant information is lacking regarding the efficacy and safety of SWD for patients with COVID-19. This study provides the first evidence that SWD is a promising adjuvant therapy for COVID-19.
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http://dx.doi.org/10.23736/S1973-9087.21.06892-1DOI Listing
May 2021

All-boron planar ferromagnetic structures: from clusters to monolayers.

Nanoscale 2021 Jun;13(21):9881-9887

Department of Physics, South China University of Technology, Guangzhou 510640, P. R. China.

Ferromagnetism in all-boron planar clusters is revealed based on high-throughput first-principles calculations. Magnetic boron clusters induced from p electrons have been confirmed with large spins, e.g., S = 3 in a B34 cluster, which can be assembled to construct all-boron ferromagnetic monolayers. Notably, the ferromagnetic semiconductors of boron monolayers can be designed with the hybridization of a nonmagnetic B36 cluster in experimental synthesis. The ferromagnetism-paramagnetism transition and semiconductor-metal transition in these boron nanostructures will occur around 500 K according to ab initio molecular dynamics simulation, indicating the potential applications in nano-devices at room temperature. The coexisting ferromagnetic and semiconducting properties in boron monolayers are attributed to the unique multicenter bonds together with the modulation of structural symmetry, which might be worth experimental attempts in the future.
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http://dx.doi.org/10.1039/d1nr00981hDOI Listing
June 2021

Vector soliton dynamics in a high-repetition-rate fiber laser.

Opt Express 2021 Apr;29(8):12049-12065

The existence of vector solitons that arise from the birefringence nature of optical fibers has been increasingly of interest for the stability of mode-locked fiber lasers, particularly for those operating in the high-fundamental-repetition-rate regime, where a large amount of fiber birefringence is required to restore the phase relation between the orthogonally polarized vector solitons, resulting in stable mode-locking free of polarization rotation. These vector solitons can exhibit diverse time-varying polarization dynamics, which prevent industrial and scientific applications requiring stable and uniform pulse trains at high fundamental repetition rates. This pressing issue, however, has so far been rarely studied. To this end, here we theoretically and experimentally dissect the formation of vector solitons in a GHz-repetition-rate fiber laser and investigate effective methods for suppressing roundtrip-to-roundtrip polarization dynamics. Our numerical model can predict both dynamic and stable regimes of high-repetition-rate mode-locking by varying the amount of fiber birefringence, resulting in the polarization rotation vector soliton (PRVS) and linearly polarized soliton (LPS), respectively. These dynamic behaviors are further studied by using an analytical approach. Interestingly, our theoretical results indicate a cavity-induced locking effect, which can be a complementary soliton trapping mechanism for the co-propagating solitons. Finally, these theoretical predications are experimentally verified, and we obtain both PRVS and LPS by adjusting the intracavity fiber birefringence.
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http://dx.doi.org/10.1364/OE.423811DOI Listing
April 2021

The Role of Oxidative Stress in Hyperuricemia and Xanthine Oxidoreductase (XOR) Inhibitors.

Oxid Med Cell Longev 2021 26;2021:1470380. Epub 2021 Mar 26.

Basic Medical College, Anhui Medical University, Hefei 230032, China.

Uric acid is the end product of purine metabolism in humans. Hyperuricemia is a metabolic disease caused by the increased formation or reduced excretion of serum uric acid (SUA). Alterations in SUA homeostasis have been linked to a number of diseases, and hyperuricemia is the major etiologic factor of gout and has been correlated with metabolic syndrome, cardiovascular disease, diabetes, hypertension, and renal disease. Oxidative stress is usually defined as an imbalance between free radicals and antioxidants in our body and is considered to be one of the main causes of cell damage and the development of disease. Studies have demonstrated that hyperuricemia is closely related to the generation of reactive oxygen species (ROS). In the human body, xanthine oxidoreductase (XOR) catalyzes the oxidative hydroxylation of hypoxanthine to xanthine to uric acid, with the accompanying production of ROS. Therefore, XOR is considered a drug target for the treatment of hyperuricemia and gout. In this review, we discuss the mechanisms of uric acid transport and the development of hyperuricemia, emphasizing the role of oxidative stress in the occurrence and development of hyperuricemia. We also summarize recent advances and new discoveries in XOR inhibitors.
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http://dx.doi.org/10.1155/2021/1470380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019370PMC
June 2021

Highly Degenerate Ground States in a Frustrated Antiferromagnetic Kagome Lattice in a Two-Dimensional Metal-Organic Framework.

J Phys Chem Lett 2021 Apr 12;12(15):3733-3739. Epub 2021 Apr 12.

Department of Physics, The Hong Kong University of Science and Technology, Hong Kong, China.

Realization of the Kagome antiferromagnetic (KAF) lattice is of high interest because the geometric frustration in the Kagome lattice is expected to give rise to highly degenerated ground states that may host exotic phases such as quantum spin liquid. Here we demonstrate the design and synthesis of a single-layer two-dimensional metal-organic framework (2D-MOF) containing a Kagome lattice of Fe(II) ions assembled on a Au(111) surface. First-principles calculations reveal that the Fe(II) ions are at a high spin state of = 2 and are coupled antiferromagnetically with nearest-neighboring exchange = 5.8 meV. The ground state comprises various degenerated spin configurations including the well-known = 0 and = √3 × √3 phases. Remarkably, we observe a spin excitation at 6 meV using tunneling spectroscopy. This work points out a feasible route toward realizing spin / KAF, a candidate quantum spin liquid system, by replacing Fe(II) by Cu(II) in the same structure.
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http://dx.doi.org/10.1021/acs.jpclett.1c00598DOI Listing
April 2021

Periplocymarin protects against myocardial fibrosis induced by β-adrenergic activation in mice.

Biomed Pharmacother 2021 Jul 8;139:111562. Epub 2021 Apr 8.

Advanced Institute for Medical Sciences, Dalian Medical University, Dalian 116044, China. Electronic address:

Periplocymarin is an effective component of Periplocae Cortex, which was wildly used as an ingredient in Traditional Chinese Medicine. Our group previously reported that periplocymarin exerted cardiotonic role via promoting calcium influx. However, its exact role in the pathogenesis of myocardial fibrosis has not been elucidated yet. The present study was aimed at determining the potential effect and underlying mechanism of periplocymarin in isoproterenol (ISO)-induced myocardial fibrosis. C57BL/6 mice were subcutaneously injected with ISO (5 mg/kg/day) or saline for 1 week. The early-to-atrial wave ratio (E/A ratio) measured by echocardiography revealed that ISO-induced heart stiffness was remarkably reversed by administration of periplocymarin (5 mg/kg/day). Masson trichrome staining exhibited that treatment of periplocymarin reduced the excessive deposition of extracellular matrix (ECM). Further investigations employing real-time PCR and western blot demonstrated that periplocymarin suppressed the expression of fibrosis related genes (Col1a1, Col3a1, Acta2 and Tgfb1) and proteins (Collagen I, Collagen III, α-SMA and TGF-β1) induced by ISO. Metabolomics analysis demonstrated that periplocymarin ameliorated the disorders triggered by ISO and many of the differential metabolic substances were involved in amino acid, glucose and lipid metabolism. Further analysis using network pharmacology revealed that three key genes, namely NOS2, NOS3 and Ptgs2, may be the potential targets of periplocymarin and responsible for the disorders. Validation using heart tissues showed that the mRNA expression of NOS3 was decreased while Ptgs2 was increased upon ISO treatment, which were reversed by periplocymarin. Moreover, the expression of COX-2 (Ptgs2 encoded protein) was consistent with the aspect of Ptgs2 mRNA, while eNOS (NOS3 encoded protein) expression was unchanged. In vitro studies exhibited that periplocymarin exerts anti-fibrotic function via regulating at least eNOS and COX-2 in cardiomyocyte. Taken together, periplocymarin protects against myocardial fibrosis induced by β-adrenergic activation, the potential mechanism was that periplocymarin targeted on, at least eNOS and COX-2, to improve the metabolic processes of cardiomyocyte and thus attenuated the myocardial fibrosis. Our study highlighted that periplocymarin is a potential therapeutic agent for the prevention of myocardial fibrosis.
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http://dx.doi.org/10.1016/j.biopha.2021.111562DOI Listing
July 2021

[A Randomized Controlled Study of Intravesical Instillation Therapy of Bacillus Calmette-Guérin vs. Epirubicinin Treating Non-muscular Invasive Bladder Cancer].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 Mar;52(2):326-333

Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.

Objective: To explore the best treatment plan of intravesical instillation for patients with non-muscular invasive bladder cancer (NMIBC), to explore recurrence-related clinicopathological factors after intravesical instillation, and to evaluate the value of the prognosis and prediction models currently used for NMIBC patients.

Methods: Starting from 2016, patients who underwent transurethral resection of bladder tumor (TURBT) in our hospital and who received post-surgery diagnosis of having intermediate or high risks for NMIBC were enrolled in the study. They were randomly assigned to different group sat a ratio of 2∶2∶1 for receiving intravesical instillation therapy of Bacillus Calmette-Guérin (BCG) for 19 times, BCG for 15 times, and epirubicin (EPI) for 18 times. The clinicopathological data of the patients were recorded before, during and after instillation therapy, and survival curves were drawn to evaluate the effects of the three regimens, using recurrence-free survival as the endpoint. Clinicopathological data were analyzed to study the associations between various factors and post-instillation recurrence. The consistency index (c-index) was used to evaluate the predictive accuracy of the scoring model of the Spanish Urological Club for Oncological Treatment (CUETO) and the risk tables of European Organization for Research and Treatment of Cancer (EORTC).

Results: A total of 93 NMIBC patients (35 in the 19-time BCG group, 37 in the 15-time BCG group, and 21 in the EPI group) were included, with a median follow-up time of 33.46 months. Twenty-two patients experienced tumor recurrence and eight, tumor progression. The survival curve showed that the BCG group had better recurrence-free survival than the EPI group ( =0.002), while the difference in recurrence-free survival between 19-time BCG and 15-time BCG groups was not statistically significant. Higher general complication rate was seen in the BCG groups compared with the EPI group (84.7% vs. 61.9%, =0.022), but there was no grade 3-5 adverse events in any group. The c-index of CUETO scoring model and EORTC risk tables was higher than that of the prediction based solely on T stage, nuclear grade, or EAU risk stratification. In addition, the c-index in the BCG group was higher than that in the whole cohort.

Conclusion: Among the subjects of this study, the recurrence rate of bladder cancer in the intravesical BCG instillation groups was lower than that of the epirubicin group. EORTC risk tables and CUETO scoring model exhibited higher predictive accuracies in BCG-treated patients than its performance for the whole NMIBC cohort.
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http://dx.doi.org/10.12182/20210360203DOI Listing
March 2021

Structural Evolution and Underlying Mechanism of Single-Atom Centers on MoC(100) Support during Oxygen Reduction Reaction.

ACS Appl Mater Interfaces 2021 Apr 31;13(14):17075-17084. Epub 2021 Mar 31.

Department of Physics, Southern University of Science and Technology, Shenzhen 518055, China.

The single-metal atoms coordinating with the surface atoms of the support constitute the active centers of as-prepared single-atom catalysts (SACs). However, under hash electrochemical conditions, (1) supports' surfaces may experience structural change, which turn to be distinct from those at ambient conditions; (2) during catalysis, the dynamic responses of a single atom to the attack of reaction intermediates likely change the coordination environment of a single atom. These factors could alter the performance of SACs. Herein, we investigate these issues using MoC(100)-supported single transition-metal (TM) atoms as model SACs toward catalyzing the oxygen reduction reaction (ORR). It is found that the MoC(100) surface is oxidized under ORR turnover conditions, resulting in significantly weakened bonding between single TM atoms and the MoC(100) surface ([email protected](100)_O* term for SAC). While the intermediate in 2 e ORR does not change the local structures of the active centers in these SACs, the O* intermediate emerging in 4 e ORR can damage [email protected] and [email protected](100)_O*. Furthermore, on the basis of these findings, we propose [email protected](100)_O* as a qualified ORR catalyst, which exhibits extraordinary 4 e ORR activity with an overpotential of only 0.33 V, surpassing the state-of-the-art Pt(111), and thus being identified as a promising alternative to the commercial Pt/C catalyst.
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http://dx.doi.org/10.1021/acsami.1c01477DOI Listing
April 2021

Modulation of plant root traits by nitrogen and phosphate: transporters, long-distance signaling proteins and peptides, and potential artificial traps.

Breed Sci 2021 Feb 25;71(1):62-75. Epub 2020 Dec 25.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.

As sessile organisms, plants rely on their roots for anchorage and uptake of water and nutrients. Plant root is an organ showing extensive morphological and metabolic plasticity in response to diverse environmental stimuli including nitrogen (N) and phosphorus (P) nutrition/stresses. N and P are two essential macronutrients serving as not only cell structural components but also local and systemic signals triggering root acclimatory responses. Here, we mainly focused on the current advances on root responses to N and P nutrition/stresses regarding transporters as well as long-distance mobile proteins and peptides, which largely represent local and systemic regulators, respectively. Moreover, we exemplified some of the potential pitfalls in experimental design, which has been routinely adopted for decades. These commonly accepted methods may help researchers gain fundamental mechanistic insights into plant intrinsic responses, yet the output might lack strong relevance to the real situation in the context of natural and agricultural ecosystems. On this basis, we further discuss the established-and yet to be validated-improvements in experimental design, aiming at interpreting the data obtained under laboratory conditions in a more practical view.
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http://dx.doi.org/10.1270/jsbbs.20102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973493PMC
February 2021

Query the trajectory based on the precise track: a Bloom filter-based approach.

Geoinformatica 2021 Mar 15:1-20. Epub 2021 Mar 15.

Ministry of Education, Key Laboratory of Virtual Geographic Environment (Nanjing Normal University), Nanjing, China.

Fast and precise querying in a given set of trajectory points is an important issue of trajectory query. Typically, there are massive trajectory data in the database, yet the query sets only have a few points, which is a challenge for the superior performance of trajectory querying. The current trajectory query methods commonly use the tree-based index structure and the signature-based method to classify, simplify, and filter the trajectory to improve the performance. However, the unstructured essence and the spatiotemporal heterogeneity of the trajectory-sequence lead these methods to a high degree of spatial overlap, frequent I/O, and high memory occupation. Thus, they are not suitable for the time-critical tasks of trajectory big data. In this paper, a query method of trajectory is developed on the Bloom Filter. Based on the gridded space and geocoding, the spatial trajectory sequences (tracks) query is transformed into the query of the text string. The geospace was regularly divided by the geographic grid, and each cell was assigned an independent geocode, converting the high-dimensional irregular space trajectory query into a one-dimensional string query. The point in each cell is regarded as a signature, which forms a mapping to the bit-array of the Bloom Filter. This conversion effectively eliminates the high degree of overlap and instability of query performance. Meanwhile, the independent coding ensures the uniqueness of the whole tracks. In this method, there is no need for additional I/O on the raw trajectory data when the track is queried. Compared to the original data, the memory occupied by this method is negligible. Based on Beijing Taxi and Shenzhen bus trajectory data, an experiment using this method was constructed, and random queries under a variety of conditions boundaries were constructed. The results verified that the performance and stability of our method, compared to R*tree index, have been improved by 2000 to 4000 times, based on one million to tens of millions of trajectory data. And the Bloom Filter-based query method is hardly affected by grid size, original data size, and length of tracks. With such a time advantage, our method is suitable for time-critical spatial computation tasks, such as anti-terrorism, public safety, epidemic prevention, and control, etc.
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http://dx.doi.org/10.1007/s10707-021-00433-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957281PMC
March 2021

Discovery of β-carboline-(phenylsulfonyl)furoxan hybrids as potential anti-breast cancer agents.

Bioorg Med Chem Lett 2021 05 17;40:127952. Epub 2021 Mar 17.

Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China. Electronic address:

The cytotoxicity properties of the β-carboline alkaloids have been broadly investigated. However, the potential application of β-carbolines was hindered due to the moderate activity in cancer. In the present study, thirty β-carboline-(phenylsulfonyl)furoxan hybrids (11a-j, 12a-j and 13a-j) were designed and synthesized through esterification and amidation reaction strategy, and their inhibitory activities against the human breast cancer cell lines MCF-7 and MDA-MB-231 were evaluated by CCK-8 assay. Biological evaluation presented that the most promising amide derivative 13h, substituted with p-methoxyphenyl group at position 1, generated high concentration of NO and evidently depressed the MCF-7 (IC = 0.89 μM) and MDA-MB-231 (IC = 0.62 μM) cells proliferation. Particularly, the wound healing and transwell assays demonstrated that 13h significantly inhibited the migration and invasion of MDA-MB-231cells. Furthermore, the preliminary mechanisms studies indicated that 13h induced G2/M phase arrest and apoptosis possibly causing by ROS accumulation and ROS-mediated DNA damage. Based on these considerations, 13h may be a promising antimetastatic agent for breast cancer, which is noteworthy for further exploration.
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http://dx.doi.org/10.1016/j.bmcl.2021.127952DOI Listing
May 2021

Robotic Mounted Rail Arm System for implementing effective workplace safety for migrant workers.

Work 2021 ;68(3):845-852

Institute of Research and Development, Duy Tan University, Da Nang, Vietnam.

Background: The selection of orders is the method of gathering the parts needed to assemble the final products from storage sites. Kitting is the name of a ready-to-use package or a parts kit, flexible robotic systems will significantly help the industry to improve the performance of this activity. In reality, despite some other limitations on the complexity of components and component characteristics, the technological advances in recent years in robotics and artificial intelligence allows the treatment of a wide range of items.

Objective: In this article, we study the robotic kitting system with a Robotic Mounted Rail Arm System (RMRAS), which travels narrowly to choose the elements.

Results: The objective is to evaluate the efficiency of a robotic kitting system in cycle times through modeling of the elementary kitting operations that the robot performs (pick and room, move, change tools, etc.). The experimental results show that the proposed method enhances the performance and efficiency ratio when compared to other existing methods.

Conclusion: This study with the manufacturer can help him assess the robotic area performance in a given design (layout and picking a policy, etc.) as part of an ongoing project on automation of kitting operations.
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http://dx.doi.org/10.3233/WOR-203418DOI Listing
June 2021

Cervical disc arthroplasty versus anterior cervical discectomy and fusion for the treatment of single-level disc degenerative disease with preoperative reversible kyphosis.

Clin Neurol Neurosurg 2021 Mar 18;202:106493. Epub 2021 Jan 18.

Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.

Objective: Whether and when cervical disc arthroplasty (CDA) could be indicated for preoperative cervical spine kyphosis is unclear. The purpose of the study was to compare the clinical and radiological outcomes of single-level CDA and single-level anterior cervical discectomy and fusion (ACDF) in the patient with preoperative reversible kyphosis.

Patients And Methods: From 2014-2018, patients who underwent single-level CDA and single-level ACDF were consecutively reviewed. The Japanese Orthopedic Association score, Neck Disability Index and VAS were used to evaluate clinical outcomes. Range of motion (ROM), C2-7 Cobb angle, functional spinal unit (FSU) angle, and heterotopic ossification (HO) were assessed.

Results: Thirty-eight CDA patients (a mean follow-up of 39.8 months) and 42 ACDF patients (37.6 months) with preoperative reversible kyphosis were included. Both groups had significant improvements in clinical outcomes, without statistically significant differences. Before surgery, there was no significant difference in cervical alignment and ROM between groups. After surgery, both groups had a significant increase in C2-7 angle and FSU without significant inter-group differences. At the last follow-up, CDA group consisted of 5 cases of lordosis, 11 cases of kyphosis and 22 cases of straight spine, while the corresponding case number in ACDF group was 4, 12 and 26 (P = 0.866). The C2-7 ROM was preserved in both groups. The segmental ROM of CDA group decreased mildly from 8.3° preoperatively to 5.1° finally, whereas the segmental ROM of ACDF group decreased significantly to nearly zero. 60.5 % (23/38) patients in CDA group developed HO with 9 levels of grade Ⅲ and 3 levels of grade Ⅳ.

Conclusion: For the patients with single-level disc degenerative disease and preoperative reversible kyphosis, both CDA and ACDF achieved satisfactory and comparable clinical results. CDA was non-inferior to ACDF regarding the radiological outcomes of cervical alignment. Patients in CDA group had a relatively high incidence of HO formation.
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http://dx.doi.org/10.1016/j.clineuro.2021.106493DOI Listing
March 2021
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