Publications by authors named "Xu Chen"

2,803 Publications

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Bidirectional anisotropic palladium nanozymes reprogram macrophages to enhance collaborative chemodynamic therapy of colorectal cancer.

Acta Biomater 2022 Aug 15. Epub 2022 Aug 15.

College of Chemistry and Materials Science, Jinan University, Guangzhou, 510632, P. R. China. Electronic address:

In the complex tumor microenvironment (TME), tumor-associated macrophages (TAMs) play an important role in immunosuppression and tumor growth; hence, tumor cells are no longer the only target during tumor treatment. However, how to simultaneously target both tumor cells and TAMs to effectively eliminate the tumor remains a challenge. Herein, based on the specific receptors for cancer cells and TAMs, we prepared bidirectional anisotropic palladium nanoclusters ([email protected] NPs) to simultaneously target tumor cells and TAMs for enhancing the therapeutic effect. In these nanoclusters, the Pd-HA part was obtained by modifying hyaluronic acid (HA) on the surface of ultra-small Pd nanozymes that could target CT26 cells. Moreover, with the high peroxidase (POD) and catalase (CAT) activity of Pd nanozymes, Pd-HA NPs directly caused cancer cell death by producing HO and highly toxic reactive oxygen therapy (ROS) through chemodynamic therapy (CDT). The other part of Pd NPs functioned as a carrier that linked mannose (Man) and the imiquimod molecule (R837) to obtain [email protected] NPs, which could specifically connect the mannose receptor of TAMs and perform targeted reprogramming of TAMs to M1 phenotype to reverse immunosuppression and further activate immunotherapy to form "double therapy". Therefore, the strategy of "double therapy" provides new sights for treating malignant tumors. STATEMENT OF SIGNIFICANCE: : How to simultaneously target tumor cells and tumor-associated macrophages (TAMs) in the tumor microenvironment remains a challenge in tumor therapy. In this study, we used a simple method to construct bidirectional anisotropic Pd nanoclusters ([email protected] NPs) that can simultaneously target tumor cells and TAMs. With the modified HA, the Pd-HA showed good targeting to CT26 cells under the effect of Pd nanozymes that can directly kill cancer cells through the production of toxic reactive oxygen species. On the other hand, with the targeting of mannose on the surface of nanoclusters, [email protected] were specifically ingested by TAMs, which induced their transformation into M1 phenotype to reverse tumor immunosuppression. Through bidirectional anisotropy, [email protected] NPs achieved the "double therapy" effect with CDT and immunotherapy on tumors.
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http://dx.doi.org/10.1016/j.actbio.2022.08.020DOI Listing
August 2022

Abnormal bile acid metabolism is an important feature of gut microbiota and fecal metabolites in patients with slow transit constipation.

Front Cell Infect Microbiol 2022 28;12:956528. Epub 2022 Jul 28.

School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Destructions in the intestinal ecosystem are implicated with changes in slow transit constipation (STC), which is a kind of intractable constipation characterized by colonic motility disorder. In order to deepen the understanding of the structure of the STC gut microbiota and the relationship between the gut microbiota and fecal metabolites, we first used 16S rRNA amplicon sequencing to evaluate the gut microbiota in 30 STC patients and 30 healthy subjects. The α-diversity of the STC group was changed to a certain degree, and the β-diversity was significantly different, which indicated that the composition of the gut microbiota of STC patients was inconsistent with healthy subjects. Among them, Bacteroides, Parabacteroides, Desulfovibrionaceae, and Ruminiclostridium were significantly upregulated, while Subdoligranulum was significantly downregulated. The metabolomics showed that different metabolites between the STC and the control group were involved in the process of bile acids and lipid metabolism, including taurocholate, taurochenodeoxycholate, taurine, deoxycholic acid, cyclohexylsulfamate, cholic acid, chenodeoxycholate, arachidonic acid, and 4-pyridoxic acid. We found that the colon histomorphology of STC patients was significantly disrupted, and TGR5 and FXR were significantly downregulated. The differences in metabolites were related to changes in the abundance of specific bacteria and patients' intestinal dysfunction. Analysis of the fecal genomics and metabolomics enabled separation of the STC from controls based on random forest model prediction [STC . control (14 gut microbiota and metabolite biomarkers)-Sensitivity: 1, Specificity: 0.877]. This study provided a perspective for the diagnosis and intervention of STC related with abnormal bile acid metabolism.
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http://dx.doi.org/10.3389/fcimb.2022.956528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366892PMC
August 2022

ProbResist: a database for drug-resistant probiotic bacteria.

Database (Oxford) 2022 Aug;2022

Department of Biology, College of Science and Technology, Wenzhou-Kean University, 88 Daxue Road, Ouhai, Wenzhou, Zhejiang 325060, China.

Drug resistance remains a global threat, and the rising trend of consuming probiotic-containing foods, many of which harbor antibiotic resistant determinants, has raised serious health concerns. Currently, the lack of accessibility to location-, drug- and species-specific information of drug-resistant probiotics has hampered efforts to combat the global spread of drug resistance. Here, we describe the development of ProbResist, which is a manually curated online database that catalogs reports of probiotic bacteria that have been experimentally proven to be resistant to antibiotics. ProbResist allows users to search for information of drug resistance in probiotics by querying with the names of the bacteria, antibiotic or location. Retrieved results are presented in a downloadable table format containing the names of the antibiotic, probiotic species, resistant determinants, region where the study was conducted and digital article identifiers (PubMed Identifier and Digital Object Identifier) hyperlinked to the original sources. The webserver also presents a simple analysis of information stored in the database. Given the increasing reports of drug-resistant probiotics, an exclusive database is necessary to catalog them in one platform. It will enable medical practitioners and experts involved in policy making to access this information quickly and conveniently, thus contributing toward the broader goal of combating drug resistance.

Database Url: https://probresist.com.
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http://dx.doi.org/10.1093/database/baac064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375527PMC
August 2022

An LSPR Sensor Integrated with VCSEL and Microfluidic Chip.

Nanomaterials (Basel) 2022 Jul 29;12(15). Epub 2022 Jul 29.

Key Laboratory of Optoelectronics Technology, Ministry of Education, Beijing University of Technology, Beijing 100124, China.

The work introduces a localized surface plasmon resonance (LSPR) sensor chip integrated with vertical-cavity surface-emitting lasers (VCSELs). Using VCSEL as the light source, the hexagonal gold nanoparticle array was integrated with anodic aluminum oxide (AAO) as the mask on the light-emitting end face. The sensitivity sensing test of the refractive index solution was realized, combined with microfluidic technology. At the same time, the finite-difference time- domain (FDTD) algorithm was applied to model and simulate the gold nanostructures. The experimental results showed that the output power of the sensor was related to the refractive index of the sucrose solution. The maximum sensitivity of the sensor was 1.65 × 10 nW/RIU, which gives it great application potential in the field of biomolecular detection.
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http://dx.doi.org/10.3390/nano12152607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370176PMC
July 2022

Oleanane-Type Triterpene Conjugates with 1-1,2,3-Triazole Possessing of Fungicidal Activity.

Molecules 2022 Aug 2;27(15). Epub 2022 Aug 2.

Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, Beijing University of Agriculture, Beijing 102206, China.

The triazole pesticide is an organic nitrogen-containing heterocyclic compound with a 1,2,3-Triazole ring. In order to develop a potential glucosamine-6-phosphate synthase (GlmS) inhibitor bactericide, 18 triazole-derivative compounds were synthesized efficiently. In addition, these compounds have not been reported in the literature. The structure was confirmed by high-resolution mass spectrometry (HRMS), 1H NMR and 13C NMR. The potential use of the most promising derivatives has been investigated by testing their antifungal activity and enzyme inhibitory activity, revealing inhibitory activities in the low micromolar range. Among them, the antifungal effects of compounds , , , , , and on were particularly significant, all of which were above 83%. These compounds will be further investigated as potential antifungal lead compounds. Their structure-activity relationships are discussed based on the effects of substituted phenyl groups on compounds.
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http://dx.doi.org/10.3390/molecules27154928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370531PMC
August 2022

Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers.

Nat Commun 2022 Aug 11;13(1):4708. Epub 2022 Aug 11.

Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.

The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.
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http://dx.doi.org/10.1038/s41467-022-32434-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372077PMC
August 2022

Effect of support type and crystal form of support in the catalytic gasification of old corrugated containers using Fe-based catalysts.

Waste Manag 2022 Aug 8;151:163-170. Epub 2022 Aug 8.

State Key Laboratory of Coal Combustion, Huazhong University of Science and Technology, 1037 Luoyu Road, 430074 Wuhan, Hubei, China.

Catalytic gasification of old corrugated containers with Fe-based catalysts is a promising way to produce renewable H along with the utilization of solid waste. In this study, the effect of support type and crystal form of support in Fe-based catalysts on the catalytic gasification of old corrugated containers was systematically investigated. The results show that, the introduction of Fe/γ-AlO, Fe/TiO, Fe/SiO, and Fe/ZSM5-30 promote H production. Among them, Fe/TiO has the highest catalytic activity on H yield (25.10 mmol/g) related to the formation of FeTiO solid-melt material. Fe/γ-AlO shows the best H selectivity (46.34 %) and good H yield (24.19 mmol/g) due to good dispersity of Fe. Further, the order of catalytic effect on H selectivity is Fe/amorphous AlO (51.46 %) > Fe/α-AlO (46.98 %) > Fe/γ-AlO (46.34 %). With the increase in cycle index, Fe/amorphous AlO shows the best catalytic effect on H yield (25.56 mmol/g) after 11 indexes due to the formation of AlFeO. Fe/γ-AlO shows the best stability on H selectivity (∼43 %) after 11 indexes.
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http://dx.doi.org/10.1016/j.wasman.2022.08.003DOI Listing
August 2022

Global burden of nonalcoholic steatohepatitis-related liver cancer, 1990-2019: a systematic analysis for the GBD 2019.

Diabetol Metab Syndr 2022 Aug 9;14(1):112. Epub 2022 Aug 9.

Department of Nutrition, School of Public Health, Sun Yat-Sen University (Northern Campus), No.74, 2nd Zhongshan Road, Guangzhou, People's Republic of China, 510080.

With the pandemic of metabolic diseases, nonalcoholic fatty liver disease (NAFLD) prevalence has dramatically elevated. NAFLD encompasses a spectrum of diseases including simple steatosis and nonalcoholic steatohepatitis (NASH), which can further progress to cirrhosis or liver cancer (LC). However, data are lacking on the burden and trend of NASH-related LC. Here, we analyzed the trends and changes of NASH-related LC burden using Global Burden of Disease (GBD) data (1990-2019). In 2019, the global incidence, prevalence, disability-adjusted life years (DALYs) and deaths of NASH related LC were 36.3 thousand (95% UI 29.5-44.9), 46.8 thousand (38.2-57.6), 796 thousand (657-976) and 34.7 thousand (28.4-43.2), respectively. The absolute numbers and rates of NASH-related LC incidence, mortality, and DALY significantly elevated from 1990 to 2019. With the age increased, the incidences, DALYs and deaths of NASH-related LC significantly elevated. The incidence and mortality rate of NASH-related LC significantly increased from 2010 to 2019 in individuals aged from 20 to 54 and older than 55 years old. We also found that a large disparity of NASH-related LC burden in different socio-demographic index (SDI) locations. The crude number and the age-standardized rate of incidences, DALYs and deaths was highest in the middle SDI locations and high SDI locations showed the largest increase of NASH-related LC burden from 1990 to 2019. Moreover, the proportion of LC incidences, deaths and DALYs attributed to NASH were 4.74%, 5.30% and 4.25%, respectively in 1990 which were increased by 43.5%, 35.3% and 49.4%, respectively in 2019. Conclusion: The global burden of NASH-related LC and the proportion LC burden attributed to NASH are significantly increasing.
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http://dx.doi.org/10.1186/s13098-022-00885-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9361538PMC
August 2022

Sequestered SQSTM1/p62 crosstalk with Keap1/NRF2 axis in hPDLCs promotes oxidative stress injury induced by periodontitis.

Free Radic Biol Med 2022 Aug 5;190:62-74. Epub 2022 Aug 5.

Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China. Electronic address:

Periodontitis is a recognized multifactorial inflammatory chronic disease, however, the exact role of oxidative stress in the pathogenesis of periodontitis is undefined. This study aims to imply the mechanism of NRF2-regulated oxidative stress and inflammatory responses under periodontitis and explored the novelty therapeutic targets. We first demonstrate that redox imbalance caused by inhibited NRF2 signaling pathway is induced in periodontium during hypoxia and bacterial events. Then we propose that LPS from P. gingivalis and hypoxia stimuli could inhibit hPDLCs proliferation and GSH level, promote ROS production, lipid peroxidation level, and pro-inflammatory cytokines such as IL-6, TNF-α, and IL-17 level caused by the inhibited PI3K/AKT/mTOR pathway and sequential sequestered crosstalk between selective autophagy SQSTM1/p62 and Keap1/NRF2 axis accompanied by the reinforced NRF2 ubiquitination degradation and inactivated NRF2 nuclear translocation. Overexpression of NRF2 and SQSTM1 can protect hPDLCs from oxidative stress and inflammation exacerbation because of enhanced NRF2 activity. Further, the antioxidant and anti-inflammation potential of puerarin is verified in vitro and in experimental periodontitis in mice through diminishing above negative feedback loop mechanically. Altogether, we speculate that NRF2-mediated redox homeostasis is a profound candidate for one of the prominent roles in periodontitis pathogenesis and suggest puerarin as a promising therapeutic target.
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http://dx.doi.org/10.1016/j.freeradbiomed.2022.08.001DOI Listing
August 2022

Sensitive and visual identification of using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use.

Front Cell Infect Microbiol 2022 22;12:949514. Epub 2022 Jul 22.

Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou, China.

is the leading cause of bacterial sexually transmitted infection (STI) and remains a major public health challenge, especially in less-developed regions. Establishing a rapid, inexpensive, and easy-to-interpret point-of-care (POC) testing system for could be critical for its treatment and limiting further transmission. Here, we devised a novel approach termed a multiple cross displacement amplification integrated with gold nanoparticle-based lateral flow biosensor (MCDA-AuNPs-LFB) for the highly specific, sensitive, user-friendly, and rapid identification of in clinical samples. A suite of MCDA primers based on the gene from 14 serological variants (serovar A-K, L1, L2, and L3) were successfully designed and used to establish the assay. Optimal assay conditions were identified at 67°C, and the detection procedure, including nucleic acid preparation (approximately 5 min), MCDA amplification (30 min), and AuNPs-LFB visual readout (within 2 min), was completed within 40 min. The all-in cost for each test was approximately $5.5 USD. The limit of detection (LoD) was 10 copies/reaction, and no cross-reaction was observed with non- microbes. A total of 135 suspected -infection genital secretion samples were collected and simultaneously detected using real-time quantitative PCR (qPCR) in our assay. Compared with the qPCR technology, the MCDA-AuNPs-LFB sensitivity, specificity, positive predictive value, and negative predictive value were 100%, 96.20%, 94.92%, and 100%, respectively. Hence, our MCDA-AuNP-LFB assay exhibited considerable potential for POC testing and could be used to identify in clinical settings, particularly in low-income regions.
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http://dx.doi.org/10.3389/fcimb.2022.949514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355032PMC
August 2022

Reduced diurnal temperature range mitigates drought impacts on larch tree growth in North China.

Sci Total Environ 2022 Aug 3:157808. Epub 2022 Aug 3.

Institut des Sciences de la Forêt tempérée, Université du Québec en Outaouais, J0V 1V0 Québec, Canada; Harvard Forest, Harvard University, 01366, MA, USA; School of Informatics and Cyber Security and Center for Ecosystem Science and Society, Northern Arizona University, 86011, AZ, USA.

Forests are facing climate changes such as warmer temperatures, accelerated snowmelt, increased drought, as well as changing diurnal temperature ranges (DTR) and cloud cover regimes. How tree growth is influenced by the changes in daily to monthly temperatures and its associations with droughts has been extensively investigated, however, few studies have focused on how changes in sub-daily temperatures i.e., DTR, influence tree growth during drought events. Here, we used a network of Larix principis-rupprechtii tree-ring data from 1989 to 2018, covering most of the distribution of planted larch across North China, to investigate how DTR, cloud cover and their interactions influence the relationship between drought stress and tree growth. DTR showed a negative correlation with larch growth in 95 % of sites (r = -0.30, significant in 42 % of sites). Cloud cover was positively correlated with growth in 87 % of sites (r = 0.13, significant in 5 % of sites). Enhanced tree growth was found at lower DTR in the absence of severe drought. Our findings highlight that in the absence of severe droughts, reduced DTR benefits tree growth, while increased cloud cover tended to benefit tree growth only during severe drought periods. Given how DTR influences drought impacts on tree growth, net tree growth was found to be larger in regions with smaller DTR.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157808DOI Listing
August 2022

Adjustment of the GRACE score by the triglyceride glucose index improves the prediction of clinical outcomes in patients with acute coronary syndrome undergoing percutaneous coronary intervention.

Cardiovasc Diabetol 2022 Aug 5;21(1):145. Epub 2022 Aug 5.

Department of Cardiology, The Third People's Hospital of Chengdu, Affiliated Hospital of Southwest Jiaotong University, Chengdu, 610014, Sichuan, China.

Background: The Global Registry of Acute Coronary Events (GRACE) score derived from clinical parameters at the time of hospital discharge is a powerful predictor of long-term mortality and reinfarction after acute coronary syndrome (ACS). The triglyceride glucose (TyG) index, which is a simple and reliable surrogate marker of insulin resistance, has been demonstrated to be an independent predictor of long-term adverse major adverse cardiac events, irrespective of diabetes mellitus. We investigate whether the addition of the TyG index improves the predictive ability of the GRACE score after percutaneous coronary intervention (PCI) in ACS patients regardless of diabetes mellitus.

Method: A retrospective cohort of 986 ACS patients undergoing PCI was enrolled in the present analyses. The GRACE score for discharge to 6 months and the TyG index were calculated. The primary endpoint was the composite of MACEs, including all-cause death and nonfatal myocardial infarction. Patients were stratified according to the primary endpoint and the tertiles of the TyG index. Cumulative curves were calculated using the Kaplan-Meier method. Multivariate Cox regression was adopted to identify predictors of MACEs. The predictive value of the GRACE score alone and combined with the TyG index or fasting blood glucose (FBG) was estimated by the area under the receiver‑operating characteristic curve, likelihood ratio test, Akaike's information criteria, continuous net reclassification improvement (NRI), and integrated discrimination improvement (IDI). Internal validation was assessed using the means of bootstrap method with 1000 bootstrapped samples.

Results: During a median follow-up of 30.72 months ((interquartile range, 26.13 to 35.07 months), 90 patients developed MACEs, more frequently in the patients with a higher TyG index. Multivariate Cox hazards regression analysis found that the TyG index, but not FBG was an independent predictor of MACEs (hazard ratio 1.6542; 95% CI 1.1555-2.3681; P = 0.006) in all types of ACS regardless of diabetes mellitus when included in the same model as GRACE score. Furthermore, Kaplan-Meier analysis revealed that the incidence of the primary endpoint rose with increasing TyG index tertiles (log-rank, P < 0.01). Adjustment the GRACE score by the TyG index improved the predictive ability for MACEs (increase in C-statistic value from 0.735 to 0.744; NRI, 0.282, 95% CI 0.028-0.426, P = 0.02; IDI, 0.019, 95% CI 0.004-0.046, P = 0.01). Likelihood ratio test showed that the TyG index significantly improved the prognostic ability of the GRACE score (χ = 12.37, 1 df; P < 0.001). The results remained consistent when the models were confirmed by internal bootstrap validation method.

Conclusion: The TyG index, but not FBG is an independent predictor of long-term MACEs after PCI in all types of ACS patients regardless of diabetes mellitus after adjusting for the GRACE score, and improves the ability of the GRACE score to stratify risk and predict prognosis of ACS patients undergoing PCI.
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http://dx.doi.org/10.1186/s12933-022-01582-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356419PMC
August 2022

Efficacy of the intraoperative opioid-sparing anesthesia on quality of patients' recovery in video-assisted thoracoscopic surgery: a randomized trial.

J Thorac Dis 2022 Jul;14(7):2544-2555

Department of Anesthesiology, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: We aimed to explore the impact of opioid-sparing anesthesia on patients' quality of recovery after video-assisted thoracoscopic surgery (VATS). We tested the primary hypothesis that our predefined opioid-sparing anesthesia provides better quality of patients' recovery compared to routine anesthesia in VATS.

Methods: Patients between 18 and 70 years, scheduled for elective VATS, had an American Society of Anesthesiologists (ASA) class I-III under general anesthesia, were randomly allocated to: routine anesthesia group and opioid-sparing anesthesia group. Patients in the opioid-sparing anesthesia group were mainly given preoperative thoracic paravertebral blockade with intraoperative withholding longer acting opioids. Patients in routine anesthesia group received opioid-based anesthesia. The primary outcome was the Quality of Recovery-15 scale (QoR-15) at 6 hours after surgery. The secondary outcomes included QoR-15 at 24 and 48 hours after surgery, Overall Benefit of Analgesia Score Satisfaction with pain treatment (OBAS) and acute pain intensity at 6, 24 and 48 hours after surgery, and clinical outcomes of recovery after surgery.

Results: A total of 159 patients were included in final analysis. The median difference in QoR-15 between opioid-sparing anesthesia and routine anesthesia was 4 (95% CI: 1-6) at 6 hours, 8 (95% CI: 4-12) at 24 hours and 4.7 (95% CI: 1-6) at 48 hours after surgery respectively; 73.4% of patient showed good recovery in opioid-sparing anesthesia group, compared to 53.8% in routine anesthesia group at 24 hours after surgery (P=0.01). Patients demonstrated lower OBAS in opioid-sparing anesthesia group compared to routine anesthesia at all time points after surgery (P<0.05). The pain at most was significantly lower in opioid-sparing anesthesia group compared to routine anesthesia at 6 and 48 hours after surgery (P<0.05). Patients exhibited faster recovery with opioid-sparing anesthesia on time to mobilize and time to first flatus (P<0.01).

Conclusions: Our intraoperative opioid-sparing anesthesia cannot improve patients' recovery at 6 hours after VATS lung surgery, but it demonstrates better outcomes at 24 hours after surgery compared to routine anesthesia, reaching to a clinically important difference.

Trial Registration: This study is registered in the Chinese Clinical Trial Registry, ChiCTR2000031609.
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http://dx.doi.org/10.21037/jtd-22-50DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344409PMC
July 2022

TAT&RGD Peptide-Modified Naringin-Loaded Lipid Nanoparticles Promote the Osteogenic Differentiation of Human Dental Pulp Stem Cells.

Int J Nanomedicine 2022 28;17:3269-3286. Epub 2022 Jul 28.

Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University School of Stomatology, Capital Medical University, Beijing, People's Republic of China.

Background: Naringin is a naturally occurring flavanone that promotes osteogenesis. Owing to the high lipophilicity, poor in vivo bioavailability, and extensive metabolic alteration upon administration, the clinical efficacy of naringin is understudied. Additionally, information on the molecular mechanism by which it promotes osteogenesis is limited.

Methods: In this study, we prepared TAT & RGD peptide-modified naringin-loaded nanoparticles (TAT-RGD-NAR-NPs), evaluated their potency on the osteogenic differentiation of human dental pulp stem cells (hDPSCs), and studied its mechanism of action through metabolomic analysis.

Results: The particle size and zeta potential of TAT-RGD-NAR-NPs were 160.70±2.05 mm and -20.77±0.47mV, respectively. The result of cell uptake assay showed that TAT-RGD-NAR-NPs could effectively enter hDPSCs. TAT-RGD-NAR-NPs had a more significant effect on cell proliferation and osteogenic differentiation promotion. Furthermore, in metabolomic analysis, naringin particles showed a strong influence on the glycerophospholipid metabolism pathway of hDPSCs. Specifically, it upregulated the expression of and (two isozymes of phospholipase A2, PLA2), increased the biosynthesis of lysophosphatidic acid (LPA).

Conclusion: These results suggested that TAT-RGD-NPs might be used for transporting naringin to hDPSCs for modulating stem cell osteogenic differentiation. The metabolomic analysis was used for the first time to elucidate the mechanism by which naringin promotes hDPSCs osteogenesis by upregulating PLA2G3 and PLA2G1B.
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http://dx.doi.org/10.2147/IJN.S371715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342892PMC
August 2022

Chiral Nanozymes for Enantioselective Biological Catalysis.

Angew Chem Int Ed Engl 2022 Aug 3. Epub 2022 Aug 3.

Changchun Institute of Applied Chemistry Chinese Academy of Sciences: Chang Chun Institute of Applied Chemistry Chinese Academy of Sciences, State Key Laboratory of Rare Earth Resource Utilization, CHINA.

As a neat combination of the characteristics of enzymatic activity and nanomaterials, nanonzymes have attracted much attention. Although tremendous effort has been devoted to developing nanozymes with high catalytic activity, substrate selectivity is often overlooked in the construction of nanozymes. With the subtly evolutional structures, natural enzymes generally possess high selectivity for chiral substrates, which play important roles in biosynthesis and biomedical applications. However, rational construction of nanozymes with high enantioselectivity remains a significant challenge. In this Minireview, we overview recent advances in synthesis strategies of chiral nanozymes and their enantioselective biological catalysis. We further focus on current challenges, potential solutions and future developing trends of chiral nanozyme-based enantioselective biological catalysis. There is plenty of room to explore. This review will provide new insights into the development of chiral nanozymes and their applications.
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http://dx.doi.org/10.1002/anie.202208757DOI Listing
August 2022

Correction to "Aptamer-Functionalized Binary-Drug Delivery System for Synergetic Obesity Therapy".

ACS Nano 2022 Jul 30. Epub 2022 Jul 30.

Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, Department of Nutrition and Health, China Agricultural University, Beijing 100191, China.

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http://dx.doi.org/10.1021/acsnano.2c04061DOI Listing
July 2022

Comparison of Outcomes of Haploidentical Peripheral Blood Stem Cell Transplantation Supported by Third-Party Cord Blood Versus Human Leukocyte Antigen-Matched Sibling Peripheral Blood Stem Cell Transplantation in Hematologic Malignancy Patients.

Front Oncol 2022 14;12:922120. Epub 2022 Jul 14.

Department of Hematology, Xiangya Hospital, Central South University, Changsha, China.

Recent studies have shown that haploidentical hematopoietic stem cell transplantation supported by third-party cord blood (haplo-cord-HSCT) results in rapid hematopoietic recovery, low incidences of graft-versus-host disease (GVHD), and relapse of hematologic malignancies. However, few reports on haploidentical peripheral blood stem cell transplantation supported by third-party cord blood (haplo-cord-PBSCT) have been published. To evaluate the outcomes of patients who underwent haplo-cord-PBSCT or human leukocyte antigen (HLA)-matched sibling donor peripheral blood stem cell transplantation (MSD-PBSCT), we retrospectively reviewed the clinical data of patients with hematologic malignancies who underwent haplo-cord-PBSCT (n = 93) or MSD-PBSCT (n = 72) in our hospital from March 2017 to December 2020. In the haplo-cord-PBSCT and MSD-PBSCT groups, the median time for neutrophil and platelet engraftment was 13 vs. 12 days ( = 0.07) and 16 vs. 13 days ( = 0.06), respectively. The 30-day cumulative incidences of neutrophil engraftment were 100.0% and 98.6% ( = 0.12). The 100-day cumulative incidences of platelet engraftment were 96.8% and 98.6% ( = 0.01). The 100-day cumulative incidences of grade II-IV and grade III-IV acute GVHD were 29.1% vs. 23.6% ( = 0.42) and 9.7% vs. 4.2% ( = 0.18). The cumulative incidences of total and moderate/severe chronic GVHD at 1 year were 26.5% vs. 17.4% and 8.1% vs. 4.5%, respectively, and at 3 years were 34.7% vs. 34.3% ( = 0.60) and 13.6% vs. 10.6% ( = 0.49), respectively. The cumulative incidences of relapse at 1 year were 9.3% and 7.2% and at 3 years were 17.0% and 17.0% ( = 0.98). Non-relapse mortality (NRM) at 1 year was 14.6% and 8.6% and at 3 years was 17.4% and 8.6% ( = 0.13) in two groups. The probabilities of overall survival (OS), disease-free survival (DFS), and GVHD-free/relapse-free survival (GRFS) at 1 year were 81.7% vs. 88.6%, 76.1% vs. 84.2%, and 71.7% vs. 79.7%, respectively, and at 3 years were 78.7% vs. 79.0%, 65.6% vs. 74.4%, and 55.5% vs. 63.6%, respectively, in the corresponding group, > 0.05. In conclusion, for patients with acute myeloid leukemia/myelodysplastic syndrome (AML/MDS) and acute lymphoid leukemia (ALL), haplo-cord-PBSCT results in similar outcomes compared with MSD-PBSCT, and it may be a valid alternative transplantation method.
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http://dx.doi.org/10.3389/fonc.2022.922120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331161PMC
July 2022

Effect of the Combining Corn Steep Liquor and Urea Pre-treatment on Biodegradation and Hydrolysis of Rice Straw.

Front Microbiol 2022 13;13:916195. Epub 2022 Jul 13.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.

A novel pre-treatment using corn steep liquor (CSL) and urea was developed to enhance the enzymatic saccharification and degradability of rice straw (RS). We used RS (1) without (Con) or with additives of (2) 5% urea (U), (3) 9% CSL and 2.5% urea (CU), and (4) 9% CSL and 5% urea (C5U). The result showed that the water-soluble carbohydrate (WSC) conversion of RS reached 69.32% after C5U pre-treatment. Scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction analysis (XRD) confirmed that the surface of pre-treated RS exposed more cellulose and hemicellulose due to the disruption of the resistant structure of lignocellulose. Pre-treated RS significantly decreased neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents and increased crude protein (CP) content, microbial colonization, and induction of and attachment. Altogether, we concluded that pre-treatment of a combination of CSL and urea has the potential to improve the nutritive value of RS.
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http://dx.doi.org/10.3389/fmicb.2022.916195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326473PMC
July 2022

A Combination of Novel Microecological Agents and Molasses Role in Digestibility and Fermentation of Rice Straw by Facilitating the Ruminal Microbial Colonization.

Front Microbiol 2022 14;13:948049. Epub 2022 Jul 14.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.

In this study, we evaluated the effect of microecological agents (MA) combined with molasses (M) on the biodegradation of rice straw in the rumen. Rice straw was pretreated in laboratory polyethylene 25 × 35 cm sterile bags with no additive control (Con), MA, and MA + M for 7, 15, 30, and 45 days, and then the efficacy of MA + M pretreatment was evaluated both and . The scanning electron microscopy, X-ray diffraction analysis, and Fourier-transform infrared spectroscopy results showed that the MA or MA + M pretreatment altered the physical and chemical structure of rice straw. Meanwhile, the ruminal microbial attachment on the surface of rice straw was significantly increased after MA+M pretreatment. Furthermore, MA + M not only promoted rice straw fermentation but also improved digestibility by specifically inducing rumen colonization of , and . Altogether, we concluded that microecological agents and molasses could be the best choices as a biological pretreatment for rice straw to enhance its nutritive value as a ruminant's feed.
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http://dx.doi.org/10.3389/fmicb.2022.948049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329086PMC
July 2022

Prions induce an early Arc response and a subsequent reduction in mGluR5 in the hippocampus.

Neurobiol Dis 2022 Jul 26;172:105834. Epub 2022 Jul 26.

Department of Pathology, University of California San Diego, La Jolla, CA, USA; Department of Medicine, University of California San Diego, La Jolla, CA, USA; Department of Pathology, Microbiology and Immunology, University of California Davis, Davis, CA, USA. Electronic address:

Synapse dysfunction and loss are central features of neurodegenerative diseases, caused in part by the accumulation of protein oligomers. Amyloid-β, tau, prion, and α-synuclein oligomers bind to the cellular prion protein (PrP resulting in the activation of macromolecular complexes and signaling at the post-synapse, yet the early signaling events are unclear. Here we sought to determine the early transcript and protein alterations in the hippocampus during the pre-clinical stages of prion disease. We used a transcriptomic approach focused on the early-stage, prion-infected hippocampus of male wild-type mice, and identify immediate early genes, including the synaptic activity response gene, Arc/Arg3.1, as significantly upregulated. In a longitudinal study of male, prion-infected mice, Arc/Arg-3.1 protein was increased early (40% of the incubation period), and by mid-disease (pre-clinical), phosphorylated AMPA receptors (pGluA1-S845) were increased and metabotropic glutamate receptors (mGluR5 dimers) were markedly reduced in the hippocampus. Notably, sporadic Creutzfeldt-Jakob disease (sCJD) post-mortem cortical samples also showed low levels of mGluR5 dimers. Together, these findings suggest that prions trigger an early Arc response, followed by an increase in phosphorylated GluA1 and a reduction in mGluR5 receptors.
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http://dx.doi.org/10.1016/j.nbd.2022.105834DOI Listing
July 2022

Nanoparticle-Based Lateral Flow Biosensor Integrated With Loop-Mediated Isothermal Amplification for Rapid and Visual Identification of for Point-of-Care Use.

Front Microbiol 2022 12;13:914620. Epub 2022 Jul 12.

Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou, China.

Chlamydial infection, caused by , is the most common bacterial sexually transmitted infection and remains a major public health problem worldwide, particularly in underdeveloped regions. Developing a rapid and sensitive point-of-care (POC) testing for accurate screening of infection is critical for earlier treatment to prevent transmission. In this study, a novel diagnostic assay, loop-mediated isothermal amplification integrated with gold nanoparticle-based lateral flow biosensor (LAMP-LFB), was devised and applied for diagnosis of in clinical samples. A set of LAMP primers based on the gene from 14 serological variants (serovar A-K, L1, L2, L3) was successfully designed and used for the development of -LAMP-LFB assay. The optimal reaction system can be performed at a constant temperature of 67°C for 35 min. The total assay process, including genomic DNA extraction (~15 min), LAMP reaction (35 min), and LFB readout (~2 min), could be finished within 60 min. The -LAMP-LFB could detect down to 50 copies/ml, and the specificity was 100%, no cross-reactions with other pathogens were observed. Hence, our -LAMP-LFB was a rapid, reliable, sensitive, cost-effective, and easy-to-operate assay, which could offer an attractive POC testing tool for chlamydial infection screening, especially in resource starvation settings.
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http://dx.doi.org/10.3389/fmicb.2022.914620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318599PMC
July 2022

A neutrophil cell membrane-biomimetic nanoplatform based on L-arginine nanoparticles for early osteoarthritis diagnosis and nitric oxide therapy.

Nanoscale 2022 Aug 18;14(32):11619-11634. Epub 2022 Aug 18.

College of Chemistry and Materials Science, Jinan University, Guangzhou, China.

Osteoarthritis (OA) is a common debilitating disease affecting articular joints for which no effective disease-modifying early diagnosis or medical therapy tools are currently available. The inefficient delivery of drugs into inflamed chondrocytes has restricted the development of anti-OA medication. Evidence has shown that inflammatory neutrophils possess the property of targeting inflammation inflammatory tissue recruiting. Herein, we report neutrophil-cell-membrane-based biomimetic nanoparticles ([email protected]) as an OA theranostic nanoplatform; they act as a NO delivery system, coating neutrophil cell membrane onto the surface of self-assembled PEGylated L-arginine nanoparticles (LANPs) to act as a NO donor and loading a Ru complex to act as a ROS inducer. [email protected] demonstrated the specific targeting of inflamed OA with low toxicity, good NO release, and excellent fluorescence/photoacoustic (FL/PA) imaging properties. We showed that [email protected] exhibited enhanced cellular association in inflamed chondrocyte cells (C28/I2), much higher than NO release from ROS oxidized LA, and it improved the inhibition of the apoptosis of inflamed C28/I2 cells compared with control treatments. studies demonstrated that [email protected] effectively targeted inflamed OA, based on real-time dual-modal FL/PA imaging, eventually exhibiting its excellent anti-inflammatory activity. Our study may provide a new approach for the early diagnosis and treatment of osteoarthritis using a neutrophil-cell-membrane-based biomimetic nanoplatform for NO or drug delivery.
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http://dx.doi.org/10.1039/d2nr02601eDOI Listing
August 2022

Gut Microbially Produced Indole-3-Propionic Acid Inhibits Atherosclerosis by Promoting Reverse Cholesterol Transport and Its Deficiency Is Causally Related to Atherosclerotic Cardiovascular Disease.

Circ Res 2022 Aug 27;131(5):404-420. Epub 2022 Jul 27.

Department of Nutrition, School of Public Health, Sun Yat-sen University (Northern Campus), Guangzhou, China (H.X., Y.Y., W.L.).

Background: Accumulating evidence has shown that disorders in the gut microbiota and derived metabolites affect the development of atherosclerotic cardiovascular disease (ASCVD). However, which and how specific gut microbial metabolites contribute to the progression of atherosclerosis and the clinical relevance of their alterations remain unclear.

Methods: We performed integrated microbiome-metabolome analysis of 30 patients with coronary artery disease (CAD) and 30 age- and sex-matched healthy controls to identify CAD-associated microbial metabolites, which were then assessed in an independent population of patients with ASCVD and controls (n=256). We further investigate the effect of CAD-associated microbial metabolites on atherosclerosis and the mechanisms of the action.

Results: Indole-3-propionic acid (IPA), a solely microbially derived tryptophan metabolite, was the most downregulated metabolite in patients with CAD. Circulating IPA was then shown in an independent population to be associated with risk of prevalent ASCVD and correlated with the ASCVD severity. Dietary IPA supplementation alleviates atherosclerotic plaque development in ApoE mice. In murine- and human-derived macrophages, administration of IPA promoted cholesterol efflux from macrophages to ApoA-I through an undescribed miR-142-5p/ABCA1 (ATP-binding cassette transporter A1) signaling pathway. Further in vivo studies demonstrated that IPA facilitates macrophage reverse cholesterol transport, correlating with the regulation of miR-142-5p/ABCA1 pathway, whereas reduced IPA production contributed to the aberrant overexpression of miR-142-5p in macrophages and accelerated the progression of atherosclerosis. Moreover, the miR-142-5p/ABCA1/reverse cholesterol transport axis in macrophages were dysregulated in patients with CAD, and correlated with the changes in circulating IPA levels.

Conclusions: Our study identify a previously unknown link between specific gut microbiota-derived tryptophan metabolite and ASCVD. The microbial metabolite IPA/miR-142-5p/ABCA1 pathway may represent a promising therapeutic target for ASCVD.
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http://dx.doi.org/10.1161/CIRCRESAHA.122.321253DOI Listing
August 2022

A Review of Woven Tracheal Stents: Materials, Structures, and Application.

J Funct Biomater 2022 Jul 16;13(3). Epub 2022 Jul 16.

College of Textiles, Donghua University, Shanghai 201620, China.

The repair and reconstruction of tracheal defects is a challenging clinical problem. Due to the wide choice of materials and structures, weaving technology has shown unique advantages in simulating the multilayer structure of the trachea and providing reliable performance. Currently, most woven stent-based stents focus only on the effect of materials on stent performance while ignoring the direct effect of woven process parameters on stent performance, and the advantages of weaving technology in tissue regeneration have not been fully exploited. Therefore, this review will introduce the effects of stent materials and fabric construction on the performance of tracheal stents, focusing on the effects of weaving process parameters on stent performance. We will summarize the problems faced by woven stents and possible directions of development in the hope of broadening the technical field of artificial trachea preparation.
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http://dx.doi.org/10.3390/jfb13030096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326637PMC
July 2022

Risk Factors for Macrosomia in Multipara: A Multi-Center Retrospective Study.

Children (Basel) 2022 Jun 22;9(7). Epub 2022 Jun 22.

Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 100034, China.

The increased incidence of macrosomia has caused an enormous burden after the transition from the almost 40-year one-child policy to the universal two-child policy in 2015 and further to the three-child policy in 2021 in China. However, studies on risk factors of macrosomia in multipara under the new fertility policy in China are limited. We aim to explore the incidence and risk factors for macrosomia in multipara to provide the scientific basis for preventing macrosomia in multipara. A multi-center retrospective study was conducted among 6200 women who had two consecutive deliveries in the same hospital and their second newborn was delivered from January to October 2018 at one of 18 hospitals in 12 provinces in China. Macrosomia was defined as birth weight ≥ 4000 g. Logistic regression models were performed to analyze risk factors for macrosomia in multipara. The incidence of macrosomia in multipara was 7.6% (470/6200) and the recurrence rate of macrosomia in multipara was 27.2% (121/445). After adjusting for potential confounders, a higher prepregnancy BMI, higher gestational weight gain, history of macrosomia, a longer gestation in the subsequent pregnancy were independent risk factors of macrosomia in multipara ( < 0.05). Healthcare education and preconception consultation should be conducted for multipara patients with a history of macrosomia to promote maintaining optimal prepregnancy BMI and avoid excessive gestational weight gain to prevent macrosomia.
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http://dx.doi.org/10.3390/children9070935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323661PMC
June 2022

Melittin Tryptophan Substitution with a Fluorescent Amino Acid Reveals the Structural Basis of Selective Antitumor Effect and Subcellular Localization in Tumor Cells.

Toxins (Basel) 2022 06 22;14(7). Epub 2022 Jun 22.

School of Pharmaceutical Sciences, Shenzhen Campus of Sun Yat-Sen University, Shenzhen 518107, China.

Melittin is a membrane-active peptide with strong anticancer activity against various cancers. Despite decades of research, the role of the singular Trp in the anticancer activity and selectivity of melittin remains poorly understood. Here, we propose a theranostic solution based on the substitution of Trp19 with a noncanonical fluorescent amino acid (Dap). The introduction of Dap residue in melittin stabilized the helical structure of the peptide, as evaluated by circular dichroism spectra and molecular dynamics simulations. In vitro hemolytic and anticancer activity assays revealed that introducing Dap residue in melittin changed its mode of action with the cell membrane, resulting in reduced hemolytic toxicity and an improved the selectivity index (SI), with up to a five-fold increase compared to melittin. In vitro fluorescence imaging of Dap-labeled melittin (MEL) in cancer cells demonstrated high membrane-penetrating activity, with strong nuclear and nucleolar localization ability. These findings provide implications for novel anticancer therapies based on Trp-substituted designs and nuclear/nucleolar targeted therapy.
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http://dx.doi.org/10.3390/toxins14070428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318513PMC
June 2022

Temporal Changes in Fecal Unabsorbed Carbohydrates Relative to Perturbations in Gut Microbiome of Neonatal Calves: Emerging of Diarrhea Induced by Extended-Spectrum β-lactamase-Producing Enteroaggregative .

Front Microbiol 2022 7;13:883090. Epub 2022 Jul 7.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Early gut microbiota development and colonization are crucial for the long-term health and performance of ruminants. However, cognition among these microbiota is still vague, particularly among the neonatal dairy calves. Here, extended-spectrum β-lactamase-producing enteroaggregative (ESBL-EAEC)-induced temporal changes in diversity, stability, and composition of gut microbiota were investigated among the neonatal female calves, with the view of discerning potential biomarkers of this arising diarrhea cases in local pastures. Nearly, 116 newborn calves were enrolled in this time period study during their first 2 weeks of life, and a total of 40 selected fecal samples from corresponding calves were used in this study. The results revealed that differentiated gut microbiome and metabolome discerned from neonatal calves were accompanied by bacterial infections over time. Commensal organisms like , and , as key microbial markers, mainly distinguish "healthy" and "diarrheic" gut microbiome. Random forest machine learning algorithm indicated that enriched fecal carbohydrates, including rhamnose and N-acetyl-D-glucosamine, and abundant short-chain fatty acids (SCFAs) existed in healthy ones. In addition, Spearman correlation results suggested that the presence of , and , key commensal bacteria of healthy calves, is positively related to high production of unabsorbed carbohydrates, SCFAs, and other prebiotics, and negatively correlated to increased concentrations of lactic acid, hippuric acid, and α-linolenic acid. Our data suggested that ESBL-EAEC-induced diarrhea in female calves could be forecasted by alterations in the gut microbiome and markedly changed unabsorbed carbohydrates in feces during early lives, which might be conducive to conduct early interventions to ameliorate clinical symptoms of diarrhea induced by the rising prevalence of ESBL-EAEC.
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http://dx.doi.org/10.3389/fmicb.2022.883090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301005PMC
July 2022

Visualization of Zika Virus Infection via a Light-Initiated Bio-Orthogonal Cycloaddition Labeling Strategy.

Front Bioeng Biotechnol 2022 8;10:940511. Epub 2022 Jul 8.

Molecular Diagnosis and Treatment Center for Infectious Diseases, Dermatology Hospital, Southern Medical University, Guangzhou, China.

Zika virus (ZIKV) is a re-emerging flavivirus that leads to devastating consequences for fetal development. It is crucial to visualize the pathogenicity activities of ZIKV ranging from infection pathways to immunity processes, but the accurate labeling of ZIKV remains challenging due to the lack of a reliable labeling technique. We introduce the photo-activated bio-orthogonal cycloaddition to construct a fluorogenic probe for the labeling and visualizing of ZIKV. Via a simple UV photoirradiation, the fluorogenic probes could be effectively labeled on the ZIKV. We demonstrated that it can be used for investigating the interaction between ZIKV and diverse cells and avoiding the autofluorescence phenomenon in traditional immunofluorescence assay. Thus, this bioorthogonal-enabled labeling strategy can serve as a promising approach to monitor and understand the interaction between the ZIKV and host cells.
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http://dx.doi.org/10.3389/fbioe.2022.940511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305201PMC
July 2022

Horizon of exosome-mediated bone tissue regeneration: The all-rounder role in biomaterial engineering.

Mater Today Bio 2022 Dec 11;16:100355. Epub 2022 Jul 11.

Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, Jiangsu, China.

Bone injury repair has always been a tricky problem in clinic, the recent emergence of bone tissue engineering provides a new direction for the repair of bone injury. However, some bone tissue processes fail to achieve satisfactory results mainly due to insufficient vascularization or cellular immune rejection. Exosomes with the ability of vesicle-mediated intercellular signal transmission have gained worldwide attention and can achieve cell-free therapy. Exosomes are small vesicles that are secreted by cells, which contain genetic material, lipids, proteins and other substances. It has been found to play the function of material exchange between cells. It is widely used in bone tissue engineering to achieve cell-free therapy because it not only does not produce some immune rejection like cells, but also can play a cell-like function. Exosomes from different sources can bind to scaffolds in various ways and affect osteoblast, angioblast, and macrophage polarization to promote bone regeneration. This article reviews the recent research progress of exosome-loaded tissue engineering, focusing on the mechanism of exosomes from different sources and the application of exosome-loaded scaffolds in promoting bone regeneration. Finally, the existing deficiencies and challenges, future development directions and prospects are summarized.
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http://dx.doi.org/10.1016/j.mtbio.2022.100355DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304878PMC
December 2022
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