Publications by authors named "Xiwen Zhang"

130 Publications

Embedded growth of colorful CsPbX3 (X = Cl, Br, I) nanocrystals in metal-organic frameworks at Room Temperature.

Nanotechnology 2022 Jan 13. Epub 2022 Jan 13.

Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Wuhan, 430074, CHINA.

Herein, we develop a novel strategy for preparing all-inorganic cesium lead halide (CsPbX3, X= Cl, Br, I) perovskite nanocrystals (NCs)@Zn-based metal-organic framework (MOF) composites through interfacial synthesis. The successful embedding of fluorescent perovskite NCs in Zn-MOFs is due to the in-situ confined growth, which is attributed to the re-nucleation of water-triggered phase transformation from Cs4PbBr6 to CsPbBr3. The controllable synthesis of mixed-halide based composites with various emission wavelength can be achieved by adding the desired amount of halide (Cl or I) salts in the re-nucleation process. More importantly, the anion exchange reaction is inhibited among various composites with different halogen atoms by being trapped in MOFs. Besides, a white light-emitting diode (WLED) is produced using a blue LED chip with the green-emitting and red-emitting composites, which has a color coordinate of (0.3291, 0.3272) and a wide color gamut. This work provides a novel route to achieving perovskite NCs growth in MOFs, which also can be extended to the other NCs embedded in frames as well.
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http://dx.doi.org/10.1088/1361-6528/ac4b2dDOI Listing
January 2022

Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system.

Synth Syst Biotechnol 2022 Mar 22;7(1):513-521. Epub 2021 Dec 22.

School of Life Science, Northeast Forestry University, Harbin, 150040, Heilongjiang, PR China.

l-phenylglycine (L-phg) is a valuable non-proteinogenic amino acid used as a precursor to β-lactam antibiotics, antitumor agent taxol and many other pharmaceuticals. L-phg synthesis through microbial bioconversion allows for high enantioselectivity and sustainable production, which will be of great commercial and environmental value compared with organic synthesis methods. In this work, an L-phg synthesis pathway was built in resulting in 0.23 mM L-phg production from 10 mM l-phenylalanine. Then, new hydroxymandelate synthases and hydroxymandelate oxidases were applied in the L-phg synthesis leading to a 5-fold increase in L-phg production. To address 2-oxoglutarate, NH , and NADH shortage, a cofactor self-sufficient system was introduced, which converted by-product l-glutamate and NAD to these three cofactors simultaneously. In this way, L-phg increased 2.5-fold to 2.82 mM. Additionally, in order to reduce the loss of these three cofactors, a protein scaffold between synthesis pathway and cofactor regeneration modular was built, which further improved the L-phg production to 3.72 mM with a yield of 0.34 g/g L-phe. This work illustrated a strategy applying for whole-cell biocatalyst converting amino acid to its value-added chiral amine in a cofactor self-sufficient manner.
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http://dx.doi.org/10.1016/j.synbio.2021.12.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715069PMC
March 2022

Comparative transcriptome and metabolome analysis reveal glutathione metabolic network and functional genes underlying blue and red-light mediation in maize seedling leaf.

BMC Plant Biol 2021 Dec 14;21(1):593. Epub 2021 Dec 14.

Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.

Background: Light quality severely affects biosynthesis and metabolism-associated process of glutathione. However, the role of specific light is still unclear on the glutathione metabolism. In this article, comparatively transcriptome and metabolome methods are used to fully understand the blue and red-light conditions working on the glutathione metabolism in maize seedling leaf.

Results: There are 20 differently expressed genes and 4 differently expressed metabolites in KEGG pathway of glutathione metabolism. Among them, 12 genes belong to the glutathione S-transferase family, 3 genes belong to the ascorbate peroxidase gene family and 2 genes belong to the ribonucleoside-diphosphate reductase gene family. Three genes, G6PD, SPDS1, and GPX1 belong to the gene family of glucose 6-phosphate dehydrogenase, spermidine synthase, and glutathione peroxidase, respectively. Four differently expressed metabolites are identified. Three of them, Glutathione disulfide, Glutathione, and l-γ-Glutamyl-L-amino acid are decreased while L-Glutamate is increased. In addition, Through PPI analysis, two annotated genes gst16 and DAAT, and 3 unidentified genes 100381533, pco105094 and umc2770, identified as RPP13-like3, BCAT-like1and GMPS, were obtained. By the analysis of protein sequence and PPI network, we predict that pco105094 and umc2770 were involved in the GSSG-GSH and AsA-GSH cycle in the network of glutathione metabolism.

Conclusions: Compared to red light, blue light remarkably changed the transcription signal transduction and metabolism of glutathione metabolism. Differently expressed genes and metabolic mapped to the glutathione metabolism signaling pathways. In total, we obtained three unidentified genes, and two of them were predicted in current glutathione metabolism network. This result will contribute to the research of glutathione metabolism of maize.
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http://dx.doi.org/10.1186/s12870-021-03376-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670197PMC
December 2021

springD2A: capturing uncertainty in disease-drug association prediction with model integration.

Bioinformatics 2021 Dec 2. Epub 2021 Dec 2.

Intelligent Data Center, School of Mathematics, Sun Yat-Sen University, Guangzhou, 510000, P.R. China.

Motivation: Drug repositioning that aims to find new indications for existing drugs has been an efficient strategy for drug discovery. In the scenario where we only have confirmed disease-drug associations as positive pairs, a negative set of disease-drug pairs is usually constructed from the unknown disease-drug pairs in previous studies, where we do not know whether drugs and diseases can be associated, to train a model for disease-drug association prediction (drug repositioning). Drugs and diseases in these negative pairs can potentially be associated, but most studies have ignored them.

Results: We present a method, springD2A, to capture the uncertainty in the negative pairs, and to discriminate between positive and unknown pairs because the former are more reliable. In springD2A, we introduce a spring-like penalty for the loss of negative pairs, which is strong if they are too close in a unit sphere, but mild if they are at a moderate distance. We also design a sequential sampling in which the probability of an unknown disease-drug pair sampled as negative is proportional to its score predicted as positive. Multiple models are learned during sequential sampling, and we adopt parameter- and feature-based ensemble schemes to boost performance. Experiments show springspringD2A is an effective tool for drug-repositioning.

Availability: A python implementation of springD2A and datasets used in this study are available at https://github.com/wangyuanhao/springD2A.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btab820DOI Listing
December 2021

Spin-constrained optoelectronic functionality in two-dimensional ferromagnetic semiconductor heterojunctions.

Mater Horiz 2021 Apr 19;8(4):1323-1333. Epub 2021 Feb 19.

School of Physics, Southeast University, Nanjing 211189, China.

Two-dimensional (2D) van der Waals (vdW) engineering has brought about many extraordinary and new physics concepts and potential applications. Herein, we propose a new type of spin-constrained optoelectronic device developed using 2D ferromagnetic semiconductor heterostructures (FMSs). It is based on a photoexcited double-band-edge transition model, involved coupling between the interlayer magnetic order and the spin-polarized band structure and can achieve the reversible switch of band alignment via reversal of magnetization. We demonstrate that such a unique magnetic optoelectronic device can be realized with a CrBr/CrCl heterojunction and other 2D FMS heterojunctions that have the same direction as the easy magnetization axis and have a switchable band alignment that allows reconfiguration. This study opens a new application window for 2D vdW heterostructures and enables the possibility for fully vdW-based ultra-compact spintronics devices.
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http://dx.doi.org/10.1039/d0mh01480jDOI Listing
April 2021

HLA-DR Expression Level in CD8 T Cells Correlates With the Severity of Children With Acute Infectious Mononucleosis.

Front Immunol 2021 3;12:753290. Epub 2021 Nov 3.

Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: This study aimed to assess the host immune signatures associated with EBV infection and its clinical value in indicating the severity of children with acute infectious mononucleosis (IM).

Methods: Twenty-eight pediatric patients with IM aged 3-8 years were enrolled. The immune phenotypes and cytokine secretion capability of T cells were detected.

Results: The percentages and absolute numbers of CD3 and CD8 T cells were significantly increased in IM patients compared with HCs. The percentages of Naïve CD4 and CD8 T cells were decreased but with increased percentages of memory CD4 and CD8 T subsets. Our results showed the upregulation of active marker HLA-DR, TCR-αβ, and inhibitory receptors PD-1, TIGIT in CD8 T cells from IM patients, which suggested that effective cytotoxic T cells were highly against EBV infection. However, EBV exposure impaired the cytokine (IFN-γ, IL-2, and TNF-α) secretion capability of CD4 and CD8 T cells after stimulation with PMA/ionomycin . Multivariate analysis revealed that the percentage of HLA-DR CD8 T cells was an independent prognostic marker for IM. The percentage of HLA-DR CD8 T cells was significantly correlated with high viral load and abnormal liver function results.

Conclusion: Robust expansion and upregulation of HLA-DR in CD8 T cells, accompanied with impaired cytokine secretion, were typical characteristics of children with acute IM. The percentage of HLA-DR CD8 T cells might be used as a prominent marker not only for the early diagnosis but also for indicating the severity of IM.
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http://dx.doi.org/10.3389/fimmu.2021.753290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596082PMC
November 2021

Circ_0003204 knockdown protects endothelial cells against oxidized low-density lipoprotein-induced injuries by targeting the miR-491-5p-ICAM1 pathway.

J Thromb Thrombolysis 2021 Nov 19. Epub 2021 Nov 19.

Department of Cardiology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University,, No. 1 Huanghe West Road, Huaiyin District, Huai'an, 223300, China.

Emerging evidence indicates that circular RNA (circRNA) is implicated in the development of atherosclerosis (AS). This study investigated the effect of circ_0003204 on endothelial cell function and explored the functional mechanism of circ_0003204 in AS progression. AS cell models were constructed by treating human umbilical vein endothelial cells (HUVEC) with oxidized low-density lipoprotein (ox-LDL). The expression of circ_0003204 was detected by quantitative real-time PCR (qPCR). The releases of pro-inflammatory factors were determined by ELISA. Cell viability was checked by CCK-8 assay. Cell apoptosis was monitored by flow cytometry assay. The ability of angiogenesis was assessed by tube formation assay. The protein levels of cell development- and apoptosis-related markers were measured by western blot. The binding relationship between miR-491-5p and circ_0003204 or intercellular adhesion molecule 1 (ICAM1) was verified by dual-luciferase reporter assay or RIP assay. The expression of circ_0003204 was strengthened in ox-LDL-treated HUVECs. Circ_0003204 knockdown inhibited ox-LDL-induced inflammation and cell apoptosis, and promoted ox-LDL-depleted cell viability and tube formation ability in HUVECs. MiR-491-5p was a target of circ_0003204, and miR-491-5p directly bound to ICAM1 3'UTR. Accordingly, circ_0003204 positively regulated ICAM1 expression by targeting miR-491-5p. Rescue experiments presented that miR-491-5p inhibition reversed the effects of circ_0003204 knockdown, and ICAM1 overexpression abolished the effects of miR-491-5p restoration. Circ_0003204 knockdown protects HUVECs against ox-LDL-induced injuries by targeting the miR-491-5p-ICAM1 pathway, hinting that circ_0003204 inhibition might prevent AS development.
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http://dx.doi.org/10.1007/s11239-021-02606-0DOI Listing
November 2021

Recombinant Human Adenovirus-p53 Therapy for the Treatment of Cervical Cancer: A Meta-Analysis.

Front Oncol 2021 18;11:748681. Epub 2021 Oct 18.

Department of Radiation, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

Objectives: To evaluate the clinical curative effects and toxicity of recombinant human adenovirus-p53 injection (rAd-p53) plus chemotherapy (CT), radiotherapy (RT), or concurrent chemoradiotherapy (CRT) for the treatment of cervical cancer.

Methods: We identified 14 eligible studies in the PubMed, Web of Science, Cochrane Library, Embase, CNKI, Wangfangdate, CBM, and VIP databases from their inception to May 2021 and performed meta-analyses using RevMan version 5.3.

Results: This analysis included 14 studies involving 737 patients. The results of the meta-analysis results showed significantly improved complete remission (odds ratio [OR] = 2.54, 95% confidence interval [CI]: 1.74-3.70, < 0.00001), partial remission (OR = 1.56, 95% CI: 1.14-2.14, = 0.006), and object response (OR = 4.47, 95% CI: 3.02-6.60, < 0.00001) rates in the rAd-p53 combination therapy group compared to those in the CT/RT/CRT group. The results of subgroup analyses of CT/RT/CRT were consistent with the overall results. Regarding the incidence of adverse reactions, only the occurrence rate of fever (OR = 18.21, 95% CI: 10.54-31.47, < 0.00001) in the rAd-p53 combination group was higher than that in the CT/RT/CRT group. No other significant differences were observed in other adverse reactions.

Conclusion: RAd-p53 combined with CT/RT/CRT for the treatment of cervical cancer showed significant advantages in efficacy and safety compared to those in the CT/RT/CRT group. Therefore, rAd-p53 has great potential as an effective therapy for cervical cancer.

Systematic Review Registration: https://inplasy.com/inplasy-2021-5-0058/.
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http://dx.doi.org/10.3389/fonc.2021.748681DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558497PMC
October 2021

Learning representation for multiple biological networks via a robust graph regularized integration approach.

Brief Bioinform 2021 Oct 5. Epub 2021 Oct 5.

Intelligent Data Center, School of Mathematics, Sun Yat-Sen University, 510275, Guangzhou, China.

Learning node representation is a fundamental problem in biological network analysis, as compact representation features reveal complicated network structures and carry useful information for downstream tasks such as link prediction and node classification. Recently, multiple networks that profile objects from different aspects are increasingly accumulated, providing the opportunity to learn objects from multiple perspectives. However, the complex common and specific information across different networks pose challenges to node representation methods. Moreover, ubiquitous noise in networks calls for more robust representation. To deal with these problems, we present a representation learning method for multiple biological networks. First, we accommodate the noise and spurious edges in networks using denoised diffusion, providing robust connectivity structures for the subsequent representation learning. Then, we introduce a graph regularized integration model to combine refined networks and compute common representation features. By using the regularized decomposition technique, the proposed model can effectively preserve the common structural property of different networks and simultaneously accommodate their specific information, leading to a consistent representation. A simulation study shows the superiority of the proposed method on different levels of noisy networks. Three network-based inference tasks, including drug-target interaction prediction, gene function identification and fine-grained species categorization, are conducted using representation features learned from our method. Biological networks at different scales and levels of sparsity are involved. Experimental results on real-world data show that the proposed method has robust performance compared with alternatives. Overall, by eliminating noise and integrating effectively, the proposed method is able to learn useful representations from multiple biological networks.
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http://dx.doi.org/10.1093/bib/bbab409DOI Listing
October 2021

Advancements in endometrial epithelial stem cell research.

Sci China Life Sci 2022 Jan 24;65(1):215-218. Epub 2021 Sep 24.

Department of Obstetrics and Gynecology, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, China.

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http://dx.doi.org/10.1007/s11427-021-1988-1DOI Listing
January 2022

Photosynthetic performance of rocket (Eruca sativa. Mill.) grown under different regimes of light intensity, quality, and photoperiod.

PLoS One 2021 27;16(9):e0257745. Epub 2021 Sep 27.

Institute of Machine Learning and Intelligent Science, Fujian University of Technology, Fuzhou, China.

In recent years, much effort has been devoted to understanding the response of plants to various light sources, largely due to advances in industry light-emitting diodes (LEDs). In this study, the effect of different light modes on rocket (Eruca sativa. Mill.) photosynthetic performance and other physiological traits was evaluated using an orthogonal design based on a combination between light intensity, quality, and photoperiod factors. Some morphological and biochemical parameters and photosynthetic efficiency of the plants were analyzed. Plants grew in a closed chamber where three light intensities (160, 190, and 220 μmol m-2 s-1) provided by LEDs with a combination of different ratios of red, green, and blue (R:G:B- 7:0:3, 3:0:7, and 5:2:3) and three different photoperiods (light/dark -10/14 h, 12/12 h, and 14/10 h) were used and compared with white fluorescent light (control). This experimental setup allowed us to study the effect of 9 light modes (LM) compared to white light. The analyzes performed showed that the highest levels of chlorophyll a, chlorophyll b, and carotenoids occurred under LM4, LM3, and LM1, respectively. Chlorophyll a fluorescence measurement showed that the best effective quantum yield of PSII photochemistry Y(II), non-photochemical quenching (NPQ), photochemical quenching coefficient (qP), and electron transport ratio (ETR) were obtained under LM2. The data showed that the application of R7:G0:B3 light mode with a shorter photoperiod than 14/10 h (light/dark), regardless of the light intensity used, resulted in a significant increase in growth as well as higher photosynthetic capacity of rocket plants. Since, a clear correlation between the studied traits under the applied light modes was not found, more features should be studied in future experiments.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0257745PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476030PMC
November 2021

Therapeutic effects of high molecular weight hyaluronic acid in severe pneumonia in ex vivo perfused human lungs.

Am J Physiol Lung Cell Mol Physiol 2021 11 15;321(5):L827-L836. Epub 2021 Sep 15.

Departments of Anesthesiology, Medicine, and Cardiovascular Research Institute, University of California, San Francisco, California.

We previously reported that extracellular vesicles (EVs) released during () bacterial pneumonia were inflammatory, and administration of high molecular weight hyaluronic acid (HMW HA) suppressed several indices of acute lung injury (ALI) from pneumonia by binding to these inflammatory EVs. The current study was undertaken to study the therapeutic effects of HMW HA in ex vivo perfused human lungs injured with (PA)103 bacterial pneumonia. For lungs with baseline alveolar fluid clearance (AFC) <10%/h, HMW HA 1 or 2 mg was injected intravenously after 1 h ( = 4-9), and EVs released during PA pneumonia were collected from the perfusate over 6 h. For lungs with baseline AFC > 10%/h, HMW HA 2 mg was injected intravenously after 1 h ( = 6). In vitro experiments were conducted to evaluate the effects of HA on inflammation and bacterial phagocytosis. For lungs with AFC < 10%/h, administration of HMW HA intravenously significantly restored AFC and numerically decreased protein permeability and alveolar inflammation from PA103 pneumonia but had no effect on bacterial counts at 6 h. However, HMW HA improved bacterial phagocytosis by human monocytes and neutrophils and suppressed the inflammatory properties of EVs released during pneumonia on monocytes. For lungs with AFC > 10%/h, administration of HMW HA intravenously improved AFC from PA103 pneumonia but had no significant effects on protein permeability, inflammation, or bacterial counts. In the presence of impaired alveolar epithelial transport capacity, administration of HMW HA improved the resolution of pulmonary edema from PA103 bacterial pneumonia.
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http://dx.doi.org/10.1152/ajplung.00626.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616617PMC
November 2021

iKeap1 activates Nrf2 signaling to protect myocardial cells from oxygen glucose deprivation/re-oxygenation-induced oxidative injury.

Biochem Biophys Res Commun 2021 10 9;574:110-117. Epub 2021 Aug 9.

Department of Cardiology, Shanghai General Hospital of Nanjing Medical University, Shanghai, China. Electronic address:

Nuclear factor E2-related factor 2 (Nrf2) activation could efficiently protect myocardial cells from oxygen glucose deprivation/re-oxygenation (OGDR). An ultra-large structure-based virtual screening has discovered iKeap1 as a novel, direct and potent Keap1 inhibitor. Here we found that iKeap1 efficiently activated Nrf2 signaling in H9c2 myocardial cells and primary murine myocardiocytes. iKeap1 induced Keap1-Nrf2 disassociation, cytosol Nrf2 protein stabilization and nuclear translocation. The antioxidant response element (ARE) activity and expression of Nrf2 cascade genes (HO1, NQO1 and GCLC) were increased in iKeap1-treated myocardial cells. In H9c2 cells and murine myocardiocytes, iKeap1 potently inhibited OGDR-induced oxidative injury by inhibiting reactive oxygen species (ROS) production, mitochondrial depolarization, lipid peroxidation and DNA damage. In addition, OGDR-induced myocardial cell death and apoptosis were largely ameliorated after pretreatment with the novel Keap1 inhibitor. Significantly, in H9c2 cells iKeap1-induced myocardial cytoprotection against OGDR was abolished with Nrf2 silencing or knockout (using CRISPR/Cas9 method). Moreover, CRISPR/Cas9-induced Keap1 knockout led to constitutive activation of Nrf2 cascade and inhibited OGDR-induced oxidative injury. Importantly, iKeap1 was unable to further protect Keap1-knockout H9c2 cells from OGDR. Together, iKeap1 activated Nrf2 signaling to protect myocardial cells from OGDR-induced oxidative injury and cell death.
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http://dx.doi.org/10.1016/j.bbrc.2021.08.024DOI Listing
October 2021

Effect of Ginsenoside Rh1 on proliferation, apoptosis, and oxidative stress in vascular endothelial cells via regulation of the Nrf2/HO-1 signaling pathway.

J Cardiovasc Pharmacol 2021 Aug 7. Epub 2021 Aug 7.

Department of Cardiology, The Affiliated Huaian NO.1 People's Hospital of Nanjing Medical University, Huaian 223300, China.

Abstract: This study aimed to investigate the role of ginsenoside Rh1 in regulating the proliferation, apoptosis, and oxidative stress in oxidized low-density lipoprotein (ox-LDL)-treated human vascular endothelial cells (VECs) and the underlying mechanisms. VECs were treated with ox-LDL to generate an in vitro atherosclerosis model. The effect of ginsenoside Rh1 on cell viability and proliferation was examined by MTT and colony formation assays, respectively, and cell apoptosis was determined by flow cytometry and TUNEL assay. The levels of reactive oxygen species, malondialdehyde, and superoxide dismutase activity were detected using biological assay. Finally, the effect of ginsenoside Rh1 on the levels of BAX and BCL-2, and the Nrf2/HO-1 signaling pathway were determined by qRT-PCR and western blot assays. Treatment with ginsenoside Rh1 significantly increased the proliferation and decreased the apoptosis of ox-LDL-treated VECs in a dose-dependent manner. Moreover, ginsenoside Rh1 also relieved oxidative stress in ox-LDL-treated VECs by activating the Nef2/HO-1 signaling pathway. Thus, ginsenoside Rh1 affects the proliferation, apoptosis, and oxidative stress in ox-LDL-treated VECs by activating the Nef2/HO-1 signaling pathway.
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http://dx.doi.org/10.1097/FJC.0000000000001121DOI Listing
August 2021

Adsorption properties of albumin and fibrinogen on hydrophilic/hydrophobic TiO surfaces: A molecular dynamics study.

Colloids Surf B Biointerfaces 2021 Nov 20;207:111994. Epub 2021 Jul 20.

Department of Engineering Mechanics, Tsinghua University, Beijing, 100084, China. Electronic address:

In serval experimental researches, UV-induced hydrophilicity enabled better hemocompatibility in the TiO surface, which was considered to be caused by the removal of the carboxylic acid contamination from the surface. In this paper, we altered the surface wetting property by applying the formate contamination on the rutile (110) surface, and systematically investigated the adsorption properties of albumin and fibrinogen on hydrophilic/hydrophobic TiO surface. Unique contacts were found between the charged residues and the hydrophilic surface, anchoring the protein on the surface. The small size and the heart shape of albumin make it easy to cross the stable water layers near the surface. Besides, albumin has a higher proportion of charged residues, so it can form more unique contacts on the hydrophilic surface. Therefore, the albumin tends to adsorb on the hydrophilic surface. For the hydrophobic surface, the water layers near the surface are weakened, which helps the fibrinogen diffusing to the surface and adjusting its orientation. Although the hydrophobic surface cannot form the unique contacts, the larger size of fibrinogen can provide more residues to form enough ordinary contacts after adjusting, and then achieves stable adsorption. Therefore, fibrinogen tends to adsorb on the hydrophobic surface.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111994DOI Listing
November 2021

Sentinel Lymph Node Mapping in Endometrial Cancer: A Comprehensive Review.

Front Oncol 2021 29;11:701758. Epub 2021 Jun 29.

Department of Gynecology and Obstetrics, Peking University People's Hospital, Beijing, China.

Endometrial cancer (EC) is known as a common gynecological malignancy. The incidence rate is on the increase annually. Lymph node status plays a crucial role in evaluating the prognosis and selecting adjuvant therapy. Currently, the patients with high-risk (not comply with any of the following: (1) well-differentiated or moderately differentiated, pathological grade G1 or G2; (2) myometrial invasion< 1/2; (3) tumor diameter < 2 cm are commonly recommended for a systematic lymphadenectomy (LAD). However, conventional LAD shows high complication incidence and uncertain survival benefits. Sentinel lymph node (SLN) refers to the first lymph node that is passed by the lymphatic metastasis of the primary malignant tumor through the regional lymphatic drainage pathway and can indicate the involvement of lymph nodes across the drainage area. Mounting evidence has demonstrated a high detection rate (DR), sensitivity, and negative predictive value (NPV) in patients with early-stage lower risk EC using sentinel lymph node mapping (SLNM) with pathologic ultra-staging. Meanwhile, SLNM did not compromise the patient's progression-free survival (PFS) and overall survival (OS) with low operative complications. However, the application of SLNM in early-stage high-risk EC patients remains controversial. As revealed by the recent studies, SLNM may also be feasible, effective, and safe in high-risk patients. This review aims at making a systematic description of the progress made in the application of SLNM in the treatment of EC and the relevant controversies, including the application of SLNM in high-risk patients.
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http://dx.doi.org/10.3389/fonc.2021.701758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276058PMC
June 2021

Photonic crystal light trapping for photocatalysis.

Opt Express 2021 Jul;29(14):22376-22402

The Achilles heel of wide-band photocatalysts such as TiO is the insufficient photogeneration in the visible range under sunlight. This has been a longstanding impediment to large-scale, real-world deployment of titania-based photocatalysis applications. Instead of traditional band engineering through heavy-doping, we suggest enhancing photocatalytic efficiency of lightly-doped TiO using photonic crystal (PC) structures. This strongly increases solar photogeneration through novel wave-interference-based light trapping. Four photocatalyst structures - simple cubic woodpile (wdp), square lattice nanorod (nrPC), slanted conical-pore (scPore), and face-centered cubic inverse opal (invop) - are optimized and compared for light harvesting in the sub- and above-gap (282 to 550 nm) regions of weakly absorbing TiO, with the imaginary part of the dielectric constant 0.01 in the visible range. The optimized lattice constants for the first three, and opal center-to-center distance for invop, are ∼300 - 350 nm. For fixed PC thickness, the ranking of visible light harvesting capability is: scPore > wdp ∼ nrPC > invop. The scPore PC deposited on highly reflective substrate is ideal for photocatalysis given its combination of enhanced light trapping and superior charge transport.
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http://dx.doi.org/10.1364/OE.427218DOI Listing
July 2021

Self-integrating current sensor for fast pulsed current monitor in transmission line.

Rev Sci Instrum 2021 Apr;92(4):045004

State Key Laboratory of Electrical Insulation and Power Equipment, Xi'an Jiaotong University, Xi'an 710049, China.

The fast linear transformer driver (FLTD) utilizes a water-insulated transmission line as its secondary. To monitor the fast pulsed current and locate the fault, a compact self-integrating current sensor is developed. Print circuit board (PCB) coils and PCB integrating resistors are used to form the current sensor. By soldering a large number of chip resistors, PCB integrating resistors with various resistance and low inductance can be obtained. The current sensor is designed in a coaxial structure to reduce its inductance and size and can be installed on the inner conductor of the FLTD's secondary water-insulated transmission line with a small opening. The principle and matching schemes for the current sensor are theoretically analyzed with the circuit principle. Both simple matching and two-stage division can be used to obtain signals without oscillation. The time constant of the two schemes is the same. However, the sensitivity of the current sensor with two-stage division is lower than that with simple matching. A 100 kV step pulse generator is used as the pulsed high current generator to verify the properties of the current sensor. The designed current sensor could respond to the step current pulse with the rise time of 4 ns. The matching scheme is verified to be effective with step response experiments. Finally, the influence of the parameters, which are the coil type, the angle between the PCB coil and the magnetic flux, the resistance of the integrating resistor, and the length of the measuring cable, on the output signal of the current sensor is studied.
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http://dx.doi.org/10.1063/5.0038730DOI Listing
April 2021

Transcriptome and Metabolomic Analyses Reveal Regulatory Networks Controlling Maize Stomatal Development in Response to Blue Light.

Int J Mol Sci 2021 May 20;22(10). Epub 2021 May 20.

College of Mechanical and Electronic Engineering, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

(1) Background: Blue light is important for the formation of maize stomata, but the signal network remains unclear. (2) Methods: We replaced red light with blue light in an experiment and provided a complementary regulatory network for the stomatal development of maize by using transcriptome and metabolomics analysis. (3) Results: Exposure to blue light led to 1296 differentially expressed genes and 419 differential metabolites. Transcriptome comparisons and correlation signaling network analysis detected 55 genes, and identified 6 genes that work in the regulation of the HY5 module and MAPK cascade, that interact with PTI1, COI1, MPK2, and MPK3, in response to the substitution of blue light in environmental adaptation and signaling transduction pathways. Metabolomics analysis showed that two genes involved in carotenoid biosynthesis and starch and sucrose metabolism participate in stomatal development. Their signaling sites located on the PHI1 and MPK2 sites of the MAPK cascade respond to blue light signaling. (4) Conclusions: Blue light remarkably changed the transcriptional signal transduction and metabolism of metabolites, and eight obtained genes worked in the HY5 module and MAPK cascade.
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http://dx.doi.org/10.3390/ijms22105393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161096PMC
May 2021

Rapid and Low-Input Profiling of Histone Marks in Plants Using Nucleus CUT&Tag.

Front Plant Sci 2021 12;12:634679. Epub 2021 Apr 12.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.

Characterizing genome-wide histone posttranscriptional modifications and transcriptional factor occupancy is crucial for deciphering their biological functions. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a powerful method for genome-wide profiling of histone modifications and transcriptional factor-binding sites. However, the current ChIP-seq experimental procedure in plants requires significant material and several days for completion. CUT&Tag is an alternative method of ChIP-seq for low-sample and single-cell epigenomic profiling using protein A-Tn5 transposase fusion proteins (PAT). In this study, we developed a nucleus CUT&Tag (nCUT&Tag) protocol based on the live-cell CUT&Tag technology. Our results indicate that nCUT&Tag could be used for histone modifications profiling in both monocot rice and dicot rapeseed using crosslinked or fresh tissues. In addition, both active and repressive histone marks such as H3K4me3 and H3K9me2 can be identified using our nCUT&Tag. More importantly, all the steps in nCUT&Tag can be finished in only 1 day, and the assay can be performed with as little as 0.01 g of plant tissue as starting materials. Therefore, our results demonstrate that nCUT&Tag is an efficient alternative strategy for plant epigenomic studies.
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http://dx.doi.org/10.3389/fpls.2021.634679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072009PMC
April 2021

DeFusion: a denoised network regularization framework for multi-omics integration.

Brief Bioinform 2021 09;22(5)

Intelligent Data Center, School of Mathematics, Sun Yat-Sen University, Guangzhou, 510275, China.

With diverse types of omics data widely available, many computational methods have been recently developed to integrate these heterogeneous data, providing a comprehensive understanding of diseases and biological mechanisms. But most of them hardly take noise effects into account. Data-specific patterns unique to data types also make it challenging to uncover the consistent patterns and learn a compact representation of multi-omics data. Here we present a multi-omics integration method considering these issues. We explicitly model the error term in data reconstruction and simultaneously consider noise effects and data-specific patterns. We utilize a denoised network regularization in which we build a fused network using a denoising procedure to suppress noise effects and data-specific patterns. The error term collaborates with the denoised network regularization to capture data-specific patterns. We solve the optimization problem via an inexact alternating minimization algorithm. A comparative simulation study shows the method's superiority at discovering common patterns among data types at three noise levels. Transcriptomics-and-epigenomics integration, in seven cancer cohorts from The Cancer Genome Atlas, demonstrates that the learned integrative representation extracted in an unsupervised manner can depict survival information. Specially in liver hepatocellular carcinoma, the learned integrative representation attains average Harrell's C-index of 0.78 in 10 times 3-fold cross-validation for survival prediction, which far exceeds competing methods, and we discover an aggressive subtype in liver hepatocellular carcinoma with this latent representation, which is validated by an external dataset GSE14520. We also show that DeFusion is applicable to the integration of other omics types.
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http://dx.doi.org/10.1093/bib/bbab057DOI Listing
September 2021

Selective solid-phase extraction of four phenylarsonic compounds from feeds, edible chicken and pork with tailoring imprinted polymer.

Food Chem 2021 Jun 12;347:129054. Epub 2021 Jan 12.

Henan Engineering Research Center of Food Material/National Demonstration Center for Experimental Food Processing and Safety Education, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China.

The novel molecularly imprinted microspheres for four phenylarsonic compounds have been firstly prepared with the reversible addition-fragmentation chain transfer polymerization in a suspension system. The resulting polymeric microspheres were characterized by infrared spectrum, scanning electron microscope and differential scanning calorimetry. With serial adsorption experiments, the polymeric microspheres showed highly specific molecular recognition, fast mass transfer rate and robust adsorption of the substrates. Then, the imprinted polymer was used as the solid-phase extraction adsorbent to extract the phenylarsonic compounds from the feeds, edible chicken and pork. The cartridge was washed with 2 mL ethyl acetate and eluted with 3 mL of methanol- acetic acid (90:10, v/v). The recoveries of the molecularly imprinted solid-phase extraction (MISPE) column ranged from 83.4% to 95.1%. This work provided a versatile approach for the specific extraction of the organoarsenic compounds from complicated matrices and exhibited a bright future for the application of MISPE column.
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http://dx.doi.org/10.1016/j.foodchem.2021.129054DOI Listing
June 2021

Chinese herbal medicine for small cell lung cancer patients: A protocol for a systematic review and meta-analysis.

Medicine (Baltimore) 2020 Dec;99(52):e23746

Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing.

Background: Small cell lung cancer (SCLC) is an aggressive disease. Chemotherapy is the standard treatment for SCLC, but the resistance and the adverse effects of Chemotherapy still remains a major problem. Although Chinese herbal medicine (traditional Chinese medicine) is wildly applied for patients with SCLC in China, the evidence of traditional Chinese medicine in the treatment for SCLC is limited.

Method: We conducted a systematic search of PubMed, EMBASE, the Chinese National Knowledge Infrastructure, the VIP Information Database, and the Wanfang Database for relevant studies. Only randomized controlled trials were included. Two investigators independently reviewed the included studies and extracted relevant data. The effect estimate of interest was the relative risk or mean difference with 95% confidence intervals.

Ethics And Dissemination: Ethical approval is not required, as this study is based on the review of published research. This review will be published in a peer-reviewed journal and disseminated both electronically and in print.

Inplasy Registration Number: INPLASY2020110055.
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http://dx.doi.org/10.1097/MD.0000000000023746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769348PMC
December 2020

XEN gel implant with or without phacoemulsification for glaucoma: a systematic review and meta-analysis.

Ann Transl Med 2020 Oct;8(20):1309

Department of Ophthalmology, Eye College of Chengdu University of TCM, Chengdu, China.

Background: XEN gel implant is an alternative method of treating glaucoma by connecting the anterior chamber and the subconjunctival space. The efficacy of the XEN gel implant and whether to combine with phacoemulsification is what most concerned. This review aims to test the efficacy and safety of the XEN gel implant compared with trabeculectomy and to test the efficacy between XEN combined with phacoemulsification and XEN alone.

Methods: The PubMed, EMBASE, the Cochrane Library, and ClinicalTrials.gov were searched through July 8, 2020. Studies comparing XEN and trabeculectomy or comparing XEN + phacoemulsification and XEN alone were included. The standard mean differences (SMD) were calculated to analyze the lowered intraocular pressure (IOP) and the number of anti-glaucoma medications. All data were measured from baseline to endpoint. The I2 statistic quantified heterogeneity ranged from 0% to 100%, and a random effects model was used in this meta-analysis. Review Manager 5.3 performed all analyses. The -test calculated all P values, and P values were regarded as statistically significant at P<0.05. The methodological index for non-randomized studies was used to find and test bias in the literature.

Results: Twelve studies with 1,602 eyes were included. Five studies compared the XEN gel implant with trabeculectomy. Eight compared XEN + phacoemulsification with XEN alone. There was no significant correlation between XEN and trabeculectomy groups in lowering IOP (SMD 0.30, 95% CI, 0.00 to 0.60, I=60%) and reduced the number of IOP lowering drugs (SMD 0.01, 95% CI, -0.16 to 0.17, I=0%). There was a significant difference between XEN + phacoemulsification and XEN alone in lowering IOP (1,034 eyes, SMD 0.22, 95% CI, 0.05 to 0.40, I=38%) and reducing the number of medications (729 eyes, SMD 0.20, 95% CI, -0.06 to 0.46, I=62%).

Conclusions: XEN gel implant, although not effective as trabeculectomy, is a safe operation for open-angle and some other types of glaucoma. Meanwhile, XEN alone is more effective than XEN combined with phacoemulsification within 1 week after operations. After three months, the two groups are similar in lowering IOP. More extensive, better-designed, strictly blinded, multicenter randomized clinical trials are needed to confirm our findings.
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http://dx.doi.org/10.21037/atm-20-6354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661862PMC
October 2020

Analysis on the Expression and Prognostic Value of LncRNA FAF in Patients with Coronary Heart Disease.

Biomed Res Int 2020 2;2020:9471329. Epub 2020 Nov 2.

Department of Cardiology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an 223000, China.

Objective: To investigate the expression and prognostic value of LncRNA FAF in patients with coronary heart disease. . 97 patients with coronary heart disease who came to our hospital were selected as the research group (RG), and 97 healthy people who came to our hospital for physical examination during the same period were selected as the control group (CG). The serum LncRNA FAF, plasma homocysteine (HCY), lipoprotein A (Lp-a), serum tumor necrosis factor (TNF-), and high-sensitivity C-reactive protein (hsCRP) in the two groups of patients were detected, and their correlations were analyzed. Then, the predictive value and risk factors of FAF for poor prognosis of patients with coronary heart disease were analyzed.

Results: The expression of LncRNA FAF in the serum of patients in the RG was significantly lower than that in the CG, and the expressions of HCY, Lp-a, TNF-, and hsCRP were significantly higher than those in the CG ( <0.05). The AUC of FAF in the diagnosis of coronary heart disease was more than 0.9. FAF was negatively correlated with the coronary lesion vessels, HCY, Lp-a, TNF-, and hsCRP expressions in patients with coronary heart disease ( < 0.05). The ROC of FAF for predicting poor prognosis in patients with coronary heart disease was greater than 0.9. Low expression of FAF; high expressions of HCY, Lp-a, and hsCRP; and increase of coronary lesion vessels were independent risk factors for poor prognosis in patients with coronary heart disease.

Conclusions: LncRNA FAF was lowly expressed in the serum of patients with coronary heart disease, and it was of high value in the diagnosis and prediction of poor prognosis of coronary heart disease. It was also an independent risk factor for poor prognosis of patients with coronary heart disease and may be a potential target for diagnosis and treatment of coronary heart disease.
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http://dx.doi.org/10.1155/2020/9471329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655239PMC
April 2021

Corrigendum to: "Protective effect of Shaoyao decoction against colorectal cancer via the Keap1-Nrf2-ARE signaling pathway", [J. Ethnopharmacol. 241 (2019) 111981].

J Ethnopharmacol 2021 Feb 6;266:113532. Epub 2020 Nov 6.

Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, No.5, Beixiange Road, Xicheng District, Beijing, 100053, China.

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http://dx.doi.org/10.1016/j.jep.2020.113532DOI Listing
February 2021

Exolytic products of alginate by the immobilized alginate lyase confer antioxidant and antiapoptotic bioactivities in human umbilical vein endothelial cells.

Carbohydr Polym 2021 Jan 27;251:116976. Epub 2020 Aug 27.

College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China; Research Center of Food Biotechnology of Xiamen City, Xiamen 361021, China; Key Laboratory of Systemic Utilization and In-Depth Processing of Economic Seaweed, Xiamen Southern Ocean Technology Center of China, Xiamen 361021, China. Electronic address:

Alginate is a natural polysaccharide resource abundant in brown algae and it can be cleaved into alginate oligosaccharides by alginate lyase. Alginate lyases and the bioactive alginate oligosaccharides have been applied in diverse fields such as pharmaceutical therapy and nutraceutical supplementation. Immobilized enzymes greatly facilitate their industrial application owing to their reusability, stability, and tunability. In this study, magnetic FeO nanoparticles were synthesized and used to immobilize an exolytic alginate lyase AlgL17 that was characterized previously. The immobilized AlgL17 demonstrated enhanced thermal and pH tolerance, extended storage stability, and moderate reusability. The mass spectrum indicated the specific activity of the immobilized AlgL17 to release alginate oligosaccharides (AOS) from alginate polysaccharide. The produced AOS exhibited their antioxidant and antiapoptotic activities in HO-stressed human umbilical vein endothelial cells by upregulation of reactive oxygen species scavenging activities and attenuation of the caspase-mediated apoptosis pathway.
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http://dx.doi.org/10.1016/j.carbpol.2020.116976DOI Listing
January 2021

Palpitation was associated with clinical outcomes in patients with hypertrophic cardiomyopathy.

Sci Rep 2020 09 10;10(1):14935. Epub 2020 Sep 10.

Department of Cardiology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Beijing West Road 6, Huai'an, 223001, China.

Hypertrophic cardiomyopathy (HCM) is a common genetic heart disease with diversified clinical presentation and it is important to identify new predictors of clinical outcomes and survival in HCM patients. In our study, 206 HCM patients were compared with respect to major adverse cardiovascular and cerebrovascular events. By multivariable logistic analysis, we determined that palpitation, together with chronic heart failure (CHF) > 1 year, was an independent predictor of major adverse cardiovascular and cerebral events (MACCE) in HCM patients (OR 3.24, 95% CI 1.60-6.57, P = 0.001). Specially, palpitation was related to higher prevalence of rehospitalization (OR 3.86, 95% CI 2.08-7.08, P < 0.001), cardiac death (OR 2.96, 95% CI 1.05-8.32, P = 0.04) and heart failure exacerbation (OR 4.07, 95% CI 2.04-8.13, P < 0.001). However, patients presented with palpitation did not show a significantly different cardiac phenotype and function. Finally, palpitation predicted a poor prognosis in HCM patients without atrial fibrillation by utilizing Kaplan-Meier analysis (P = 0.041). In conclusion, palpitation could be a new predictor of clinical outcomes and overall survival in HCM patients.
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http://dx.doi.org/10.1038/s41598-020-71797-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483715PMC
September 2020

Overexpressing TGF-β1 in mesenchymal stem cells attenuates organ dysfunction during CLP-induced septic mice by reducing macrophage-driven inflammation.

Stem Cell Res Ther 2020 09 3;11(1):378. Epub 2020 Sep 3.

Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009, China.

Background: Sepsis remains a leading cause of death in critically ill patients. It is well known that mesenchymal stem cells (MSCs) are a promising therapy partly due to their paracrine-mediated immunoregulatory function. Previous study demonstrated that transforming growth factor-beta1 (TGF-β1) is an important cytokine secreted by MSCs and that it participates in MSC-mediated macrophage phenotype switch from pro-inflammatory to pro-resolution. In addition, the transformation of macrophage phenotype may be a potential treatment for sepsis. However, the therapeutic effect of overexpressing TGF-β1 in MSCs (MSC-TGF-β1) on sepsis is not well understood. Therefore, this study aimed to evaluate the effects of TGF-β1 overexpressing MSCs on organ injury in cecal ligation and puncture (CLP)-induced septic mice and to detect the changes in macrophage phenotype during this process.

Methods: Mouse MSCs stably transfected with TGF-β1 were constructed and injected into CLP-induced septic mice via tail vein. After 24 h, the mice were sacrificed; then, the histopathology of the organ was evaluated by hematoxylin-eosin (H&E) staining. Inflammatory cytokines were detected by ELISA. Macrophage infiltration and phenotype transformation in the tissues were determined by immunohistochemistry and flow cytometry. In addition, we performed adoptive transfer of mouse peritoneal macrophage pretreated with TGF-β1 overexpressing MSCs in septic mice.

Results: We found that infusion of TGF-β1 overexpressing MSCs attenuated the histopathological impairment of the organ, decreased the pro-inflammatory cytokine levels and inhibited macrophage infiltration in tissues. TGF-β1 overexpressing MSCs induced macrophage phenotypes changed from pro-inflammatory to pro-resolution in inflammatory environment. The adoptive transfer of mouse peritoneal macrophages pretreated with TGF-β1 overexpressing MSCs also relieved organ damage in CLP-induced septic mice.

Conclusion: Under septic conditions, TGF-β1 overexpressing MSCs can enhance the therapeutic effects of MSCs on organ injury and inflammation as a result of reduced macrophage infiltration and induced macrophages transformation, the adoptive transfer of macrophages treated with TGF-β1 overexpressing MSCs also relieved organ damage. This will provide new hope for the treatment of sepsis.
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http://dx.doi.org/10.1186/s13287-020-01894-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469348PMC
September 2020

Efficacy and safety of TCM combined with chemotherapy for SCLC: a systematic review and meta-analysis.

J Cancer Res Clin Oncol 2020 Nov 14;146(11):2913-2935. Epub 2020 Aug 14.

Department of Oncology, China Academy of Chinese Medical Sciences Guang'anmen Hospital, Beijing, China.

Background: Chemotherapy is the standard treatment for small cell lung cancer (SCLC), but chemotherapy resistance and adverse reactions remain major problems. Although Traditional Chinese Medicine (TCM) is wildly applied for patients with SCLC in China, the evidence of TCM in the treatment for SCLC is limited.

Purpose: To evaluate the efficacy and safety of TCM combined with chemotherapy for patients with SCLC.

Method: We conducted a systematic search of PubMed, EMBASE, the Chinese National Knowledge Infrastructure, the VIP Information Database, and the Wanfang Database for randomized-controlled trials (RCTs) that are relevant. The included studies were reviewed by two investigators, with relevant data extracted independently. The effect estimate of interest was the relative risk (RR) or mean difference with 95% confidence intervals (95% CIs).

Results: 22 RCTs involving 1887 patients were included in this study. Compared with patients treated with chemotherapy© alone, those with Chinese herbal medicine and chemotherapy (TCM-C) had better therapeutic effects (RR = 1.295, 95% CI 1.205-1.391, P < 0.001), KPS scores (RR = 1.310, 95% CI 1.210-1.418, P < 0.001), 1-year survival rate (RR = 1.282, 95% CI 1.129-1.456, P < 0.001), 3-year survival rate (RR = 2.109, 95% CI 1.514-2.939, P < 0.001), and 5-year survival rate (RR = 2.373, 95% CI 1.227-4.587, P = 0.01). The incidence of gastrointestinal reaction (RR of = 0.786, 95% CI 0.709-0.870, P < 0.000) and bone marrow depression (RR = 0.837, 95% CI 0.726-0.965, P = 0.014) in TCM-C group were lower than that in the C group.

Conclusion: The systematic review indicated that TCM combined with chemotherapy may improve therapeutic effect, quality of life, and prolong survival time. More large-scale and higher quality RCTs are warranted to support our findings.

Prospero Registration Number: CRD42016038016.
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http://dx.doi.org/10.1007/s00432-020-03353-0DOI Listing
November 2020
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