Publications by authors named "Xiuyuan Chen"

24 Publications

  • Page 1 of 1

Mental Health Help-Seeking and Associated Factors Among Public Health Workers During the COVID-19 Outbreak in China.

Front Public Health 2021 11;9:622677. Epub 2021 May 11.

School of Public Health, Sun Yat-sen University, Guangzhou, China.

The COVID-19 outbreak in China has created multiple stressors that threaten individuals' mental health, especially among public health workers (PHW) who are devoted to COVID-19 control and prevention work. This study aimed to investigate the prevalence of mental help-seeking and associated factors among PHW using Andersen's Behavioral Model of Health Services Use (BMHSU). A cross-sectional survey was conducted among 9,475 PHW in five provinces across China between February 18 and March 1, 2020. The subsample data of those who reported probable mental health problems were analyzed for this report ( = 3,417). Logistic and hierarchical regression analyses were conducted to examine the associations of predisposing, enabling, need, and COVID-19 contextual factors with mental health help-seeking. Only 12.7% of PHW reported professional mental help-seeking during the COVID-19 outbreak. PHW who were older, had more days of overnight work, received psychological training, perceived a higher level of support from the society, had depression and anxiety were more likely to report mental help-seeking (OR range: 1.02-1.73, all < 0.05) while those worked in Centers for Disease Control and Prevention were less likely to seek help (OR = 0.57, < 0.01). The belief that mental health issues were not the priority (64.4%), lack of time (56.4%), and shortage of psychologists (32.7%) were the most frequently endorsed reasons for not seeking help. The application of BMHSU confirmed associations between some factors and PHW's mental health help-seeking. Effective interventions are warranted to promote mental health help-seeking of PHW to ameliorate the negative impact of mental illness and facilitate personal recovery and routine work.
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http://dx.doi.org/10.3389/fpubh.2021.622677DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144452PMC
June 2021

Lung cancer organoids analyzed on microwell arrays predict drug responses of patients within a week.

Nat Commun 2021 05 10;12(1):2581. Epub 2021 May 10.

Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.

While the potential of patient-derived organoids (PDOs) to predict patients' responses to anti-cancer treatments has been well recognized, the lengthy time and the low efficiency in establishing PDOs hamper the implementation of PDO-based drug sensitivity tests in clinics. We first adapt a mechanical sample processing method to generate lung cancer organoids (LCOs) from surgically resected and biopsy tumor tissues. The LCOs recapitulate the histological and genetic features of the parental tumors and have the potential to expand indefinitely. By employing an integrated superhydrophobic microwell array chip (InSMAR-chip), we demonstrate hundreds of LCOs, a number that can be generated from most of the samples at passage 0, are sufficient to produce clinically meaningful drug responses within a week. The results prove our one-week drug tests are in good agreement with patient-derived xenografts, genetic mutations of tumors, and clinical outcomes. The LCO model coupled with the microwell device provides a technically feasible means for predicting patient-specific drug responses in clinical settings.
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http://dx.doi.org/10.1038/s41467-021-22676-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110811PMC
May 2021

Robot-assisted minimally invasive transforaminal lumbar interbody fusion versus open transforaminal lumbar interbody fusion: a retrospective matched-control analysis for clinical and quality-of-life outcomes.

J Comp Eff Res 2021 Jul 28;10(10):845-856. Epub 2021 Apr 28.

Department of Spine Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

To compare the screw accuracy and clinical outcomes between robot-assisted minimally invasive transforaminal lumbar interbody fusion (RA MIS-TLIF) and open TLIF in the treatment of one-level lumbar degenerative disease. From May 2018 to December 2019, a consecutive series of patients undergoing robot-assisted minimally invasive one-level lumbar fusion procedures were retrospectively compared with matched controls who underwent one-level open TLIF procedures for clinical and quality-of-life outcomes. A total of 52 patients underwent RA MIS-TLIF procedures (robot-assisted [RA] group) and 52 matched controls received freehand open TLIF procedures (open [OP] group). The RA group had more grade A screws with 96.2% one-time success rate of screw placement (p < 0.05). Besides, the RA group experienced less intraoperative blood loss and shorter length of hospital stay, while the OP group had shorter operative duration and cumulative radiation time (p < 0.001). What is more, the average VAS score for low back pain and ODI score in the RA group were lower than that in the OP group 1 month after operation (p < 0.05). The use of real-time, image-guided robot system may further expand the advantages of MIS-TLIF technique in terms of accuracy and safety.
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http://dx.doi.org/10.2217/cer-2021-0078DOI Listing
July 2021

Inhibition of human UDP-glucuronosyltransferase enzyme by belinostat: Implications for drug-drug interactions.

Toxicol Lett 2021 Mar 5;338:51-57. Epub 2020 Dec 5.

School of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin, 124221, China. Electronic address:

Belinostat is a pan-histone deacetylase (HDAC) inhibitor which recently approved for the treatment of relapsed/refractory Peripheral T-cell lymphomas (PTCL). To assess drug-drug interactions (DDIs) potential of belinostat via inhibition of UDP-glucuronosyltransferases (UGTs), the effects of belinostat on UGTs activities were investigated using the non-selective probe substrate 4-methylumbelliferone (4-MU) and trifluoperazine (TFP) by UPLC-MS/MS. Belinostat exhibited a wide range of inhibition against UGTs activities, particularly a potent non-competitive inhibition against UGT1A3, and weak inhibition against UGT1A1, 1A7, 1A8, 2B4 and 2B7. Further, in vitro-in vivo extrapolation (IVIVE) approaches were used to predict the risk of DDI arising from inhibition of UGTs. Our data indicate that the intravenous infusion of belinostat at clinical available dose can contribute a significant increase to the AUC of co-administrated drugs primarily cleared by UGT1A3 or UGT1A1, which will result in potential DDIs. In contrast, oral administrated belinostat is unlikely to cause significant DDIs through inhibition of glucuronidation.
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http://dx.doi.org/10.1016/j.toxlet.2020.12.001DOI Listing
March 2021

Clinical value of serum biomarkers CA153, CEA, and white blood cells in predicting sentinel lymph node metastasis of breast cancer.

Int J Clin Exp Pathol 2020 1;13(11):2889-2894. Epub 2020 Nov 1.

Department of Breast Surgery, West China Hospital of Sichuan University Chengdu, Sichuan, China.

We retrospectively analyzed preoperative serum CA153, carcinoembryonic antigen (CEA) and white blood cells (WBC) in 121 breast cancer patients who underwent breast-conserving therapy and sentinel lymph node biopsy (SLNB) between June 2017 and April 2019 in our institution. The receiver operating characteristic curve (ROC curve) was used to determine the optional cut-offs of these biomarkers for predicting SLN metastasis. The relationship between the parameters to SLN metastasis of breast cancer was assessed by univariate analysis and multivariate logistic regression models. We finally enrolled 121 breast cancer patients who all underwent SLNB, of whom 56 were confirmed as positive SLN by histopathology. ROC curve analysis calculated an ideal CA153 cutoff value of 7.85 U/ml in prediction of SLN metastasis, with a sensitivity of 73.2%, and specificity of 67.7%. The ideal cutoff value for CEA to predict SLN metastasis was 1.66 ng/ml (sensitivity 67.9%, specificity 73.8%). CA153 combined with CEA showed specificity 78.6% and specificity 76.9%. CA153 and CEA combined with WBC presented sensitivity 80.4% and specificity 78.5%. In the multivariate logistic regression analysis, CA153 (odds ratio (OR): 1.165, 95% confidence interval (CI) 1.061-1.279, P<0.001) and CEA (OR: 3.440, 95% CI: 1.859-6.366, P<0.001) were independent predictive factors of SLN metastasis in patients with breast cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716139PMC
November 2020

Selective thoracolumbar/lumbar fusion for Syringomyelia-associated scoliosis: a case-control study with Lenke 5C adolescent idiopathic scoliosis.

BMC Musculoskelet Disord 2020 Nov 14;21(1):749. Epub 2020 Nov 14.

Department of Spine Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, No.160 Pujian Road, Shanghai, 200120, China.

Background: Selective thoracolumbar/lumbar fusion technique was introduced to treat adolescent idiopathic scoliosis (AIS) patients with major thoracolumbar/lumbar curves. Theoretically, this surgical strategy could also be applied to syringomyelia patients. No previous study has specifically addressed the effectiveness of selective thoracolumbar/lumbar fusion for patients with syringomyelia-associated scoliosis. The aim of the study was to investigate the effectiveness of selective thoracolumbar/lumbar fusion for the surgical treatment of patients with syringomyelia-associated scoliosis.

Methods: From February 2010 to September 2016, 14 syringomyelia-associated patients with major thoracolumbar/lumbar curves were retrospectively reviewed. Besides, 30 Lenke 5C AIS patients were enrolled as a control group. Posterior selective thoracolumbar/lumbar fusion was performed for both groups. Patients' demographic, operative, radiological, and quality of life data were reviewed with follow-up. Intragroup comparisons were performed for each parameter.

Results: The two groups were matched by age, gender, curve characteristics, duration of follow-up, and all preoperative radiographic parameters except for thoracic kyphosis. After surgery, the average correction rate of the major thoracolumbar/lumbar curve was 82.2 ± 7.8% in the syringomyelia group, which was not significantly different from that of AIS group (82.5 ± 10.6%, P = 0.47). A similar improvement of unfused thoracic curve was observed between the two groups (50.1 ± 16.5% vs. 48.5 ± 26.9%, P = 0.29). During the follow-up, the correction effect of scoliosis was well maintained, without aggravation of the original neural symptoms or fresh permanent neurological deficits. Of note, the number of fusion levels was significantly larger in syringomyelia group than that in AIS group (7.6 ± 1.4 vs. 6.5 ± 1.2, P < 0.01). The average follow up was 47.6 months (36-81 months).

Conclusion: Similar to AIS cases, syringomyelia-associated scoliosis can be effectively and safely corrected by selective thoracolumbar/lumbar fusion with satisfactory surgical outcomes. However, the syringomyelia group, on average, required an additional fused segment for treatment as compared to the AIS group (7.6 versus 6.5 in the AIS group).
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http://dx.doi.org/10.1186/s12891-020-03779-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666459PMC
November 2020

Cervical Angina: A Literature Review on Its Diagnosis, Mechanism, and Management.

Asian Spine J 2020 Oct 29. Epub 2020 Oct 29.

Department of Spine Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Cervical angina has been defined as chest pain that resembles true cardiac angina but originates from the disorders of the cervical spine. Thus, physicians and spine surgeons alike should raise awareness of this unusual condition for diagnosis and treatment. Particularly when neurologic signs and symptoms are present, there should be a strong suspicion for cervical angina in any patient with inadequately explained noncardiac chest pain. Cervical angina can be diagnosed according to negative cardiac workups, positive neurologic examination, and cervical radiographic findings (herniated disk, spinal cord compression, or foraminal encroachment). However, the mechanisms of pain production in cervical angina remain unclear. Previous studies attributed the pain to cervical nerve root compression, cervical sympathetic afferent fibers, referred pain, or lesions of the posterior horn of the spinal cord. Conservative treatments, which include neck collar fixation, head traction, and nonsteroidal anti-inflammatory drugs, have been determined to be successful in most patients with cervical angina. But when conservative treatment fails, anterior cervical surgery with complete decompression of the spinal cord and/or nerve root has been identified to effectively relieve cervical angina symptoms.
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http://dx.doi.org/10.31616/asj.2020.0269DOI Listing
October 2020

Right diaphragm metastasis of endometrial cancer: a case report.

J Int Med Res 2020 Oct;48(10):300060520961706

Department of Thoracic Surgery, Peking University People's Hospital, Beijing, P.R. China.

A diaphragmatic tumor is usually caused by metastasis from lung cancer, malignant mesothelioma, and malignant thymoma. Endometrial cancer is rarely involved in metastasis to the diaphragm. A right anterior mediastinal tumor was found in a 60-year-old woman who was initially diagnosed with endometrial carcinoma. There was initially no relationship between the right anterior mediastinal tumor and endometrial carcinoma. Radical curative surgery was performed for endometrial carcinoma. The endometrial carcinoma stage was IA. The patient was admitted to the Department of Thoracic Surgery 6 months after the curative surgery. Intraoperative exploration showed a tumor growing in the right diaphragm. Right diaphragmotomy was performed. Immunohistochemistry showed metastasis of endometrial carcinoma to the diaphragm. Endometrial cancer solitary metastasis to the diaphragm is rare. Clinicians should be aware of this possibility. Surgical treatment followed by a pathological examination is the most useful method for determining the diagnosis of a diaphragmatic tumor due to metastasis of endometrial cancer.
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http://dx.doi.org/10.1177/0300060520961706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556180PMC
October 2020

Robot-assisted orthopedic surgery in the treatment of adult degenerative scoliosis: a preliminary clinical report.

J Orthop Surg Res 2020 Jul 25;15(1):282. Epub 2020 Jul 25.

Department of Spine Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 160 Pujian Road, Shanghai, 200127, China.

Study Design: A single-institution, retrospective cohort study.

Objective: To compare the accuracy and short-term clinical outcomes of pedicle screw placement between robot-assisted (RA) and freehand (FH) technique in the treatment of adult degenerative scoliosis (ADS).

Methods: From February 2018 to October 2019, 97 adult patients with degenerative scoliosis admitted to our department were retrospectively reviewed. Thirty-one patients received robot-assisted pedicle screw placement (RA group), and 66 patients underwent freehand pedicle screw placement (FH group). Patient demographics and short-term clinical outcomes were recorded and compared between two groups. Gertzbein-Robbins grading system was adopted to evaluate the accuracy of pedicle screw placement by means of postoperative CT scan. Short-term clinical outcomes consist of operative time, intraoperative blood loss, length of hospital stay (LOS), radiological parameters, Scoliosis Research Society-22 (SRS-22) scores before the operation, 6 months after operation, adverse events, and revisions.

Results: The accuracy of screw placement was higher than that of the FH group (clinically acceptable 98.7% vs. 92.2%; P< 0.001). Intraoperative blood loss of the RA group was less than those in the FH group (499 vs. 573 ml; P < 0.001). Operative time (283.1 vs. 291.9 min; P = 0.31) and length of stay (12.8 vs. 13.7 days; P = 0.36) were compared between RA and FH groups. In terms of radiological parameters, both of groups were improved postoperatively. The SRS-22 scores at 6 months after operation from both groups were better than those before operation. For surgery-related complication, one case had pressure sores in the RA group while two cases developed dural tears in the FH group. No revision was required in both groups.

Conclusion: Combined with other surgical correction modalities, robot-assisted pedicle screw fixation is an effective and safe method of treating degenerative scoliosis. Due to its satisfactory surgical outcomes such as higher accuracy and less trauma, it provides a good alternative for clinical practice.

Level Of Evidence: 3.
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http://dx.doi.org/10.1186/s13018-020-01796-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382042PMC
July 2020

Validation of a serum 4-microRNA signature for the detection of lung cancer.

Transl Lung Cancer Res 2019 Oct;8(5):636-648

School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.

Background: Our previous studies have identified a serum-based 4-microRNA (4-miRNA) signature that may help distinguish patients with lung cancer (LC) from non-cancer controls (NCs). Here, we used an extended independent cohort of 398 subjects to further validate the diagnostic ability of this 4-miRNA signature.

Methods: Using quantitative reverse transcription polymerase chain reaction (qRT-PCR), expression of the 4-miRNAs was assessed in a total of 398 sera that included 213 LC patients and 185 NCs. A logistic regression model using training-test sets, receiver operating characteristic (ROC) curve analysis and t-test were used to test the impact of varying expression of these miRNAs on its diagnostic accuracy for LC. The cell proliferation and colony formation affected by these miRNAs, as well as gene ontology (GO) analysis of miRNA target genes were performed.

Results: The levels of the 4-miRNAs were significantly higher in the serum of patients with LCs as compared to NCs. Using a logistic regression prediction model based on training and test sets analysis, we obtained the area under the curve (AUC) of 0.921 [95% confidence interval (CI), 0.876-0.966] on the test set with specificity 90.6%, sensitivity 77.9%, accuracy 84.1%, positive predictive value (PPV) 89.8% and negative predictive value (NPV) 79.5%.

Conclusions: We have verified that this serum 4-miRNA signature could provide a promising noninvasive biomarker for the prediction of LC, particularly in patients with indeterminate lung nodules on screening CT scans.
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http://dx.doi.org/10.21037/tlcr.2019.09.11DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835096PMC
October 2019

Development of a Novel Three Degrees-of-Freedom Rotary Vibration-Assisted Micropolishing System Based on Piezoelectric Actuation.

Micromachines (Basel) 2019 Jul 29;10(8). Epub 2019 Jul 29.

Changchun Equipment and Technology Research Institute, Norinco Group, Changchun 130012, China.

The limited degrees of freedom (DOF) and movement form of the compliant vibration-assisted processing device are inherent constraints of the polishing technique. In this paper, a concept of a 3-DOF rotary vibration-assisted micropolishing system (3D RVMS) is proposed and demonstrated. The 3-DOF means the proposed vibration-assisted polishing device (VPD) is driven by three piezo-electric (PZT) actuators. Compared with the current vibration-assisted polishing technology which generates a trajectory with orthogonal actuators or parallel actuators, a novel 3-DOF piezoelectrically actuated VPD was designed to enable the workpiece to move along the rotational direction. Meanwhile, the proposed VPD can deliver large processing stoke in mrad scale and can be operated at a flexible non-resonant mode. A matrix-based compliance modeling method was adopted for calculating the compliance and amplification ratio of the VPD. Additionally, the dynamic and static properties of the developed VPD were verified using finite element analysis. Then, the VPD was manufactured and experimentally tested to investigate its practical performance. Finally, various polished surfaces which used silicon carbide (SiC) ceramic as workpiece material were uniformly generated by the high-performance 3D RVMS. Compared with a nonvibration polishing system, surface roughness was clearly improved by introducing rotary vibration-assisted processing. Both the analysis and experiments verified the effectiveness of the present 3D RVMS for micro-machining surfaces.
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http://dx.doi.org/10.3390/mi10080502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722868PMC
July 2019

Circular RNA modulates autophagy via -STAT3-PRKAA/AMPKα signaling in STK11 mutant lung cancer.

Autophagy 2020 04 28;16(4):659-671. Epub 2019 Jun 28.

School of Medicine, Southern University of Science and Technology, Shenzhen, China.

The role of circular RNA in cancer is emerging. A newly reported circular RNA ( is critical in cell proliferation of various cancer types, although its role in non-small cell lung cancer (NSCLC), has yet to be elucidated. Our results provided evidence that silencing of significantly impaired cell proliferation, migration, invasion and induced macroautophagy/autophagy. Mechanistically, we uncovered that autophagy was induced upon loss of via the -STAT3-PRKAA/AMPKa axis in STK11 mutant lung cancer cell lines (A549 and H838). STAT3 abrogation as well as transfection with a mimic, recapitulated the induction of autophagy. We also demonstrated antagonistic regulation on autophagy between and linear (). We therefore propose that the ratio between and (C:L ratio) may reflect autophagy levels in cancer cells. We observed that a high C:L ratio (>0.49) was an indicator of poor survival, especially in advanced-stage NSCLC patients. These results support that is a key autophagy regulator in a subset of lung cancer and has potential clinical use as a prognostic factor. The circular RNA ( functions as an oncogene and autophagy regulator may potential use as a prognostic marker and therapeutic target in lung cancer. 3-MA: 3-methyladenine; AMPK: AMP-activated protein kinase; ATG7: autophagy related 7; Baf-A: bafilomycin A; BECN1: beclin 1; : circular HIPK3; CQ: chloroquine; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFP: green fluorescent protein; HIPK3: homeodomain interacting protein kinase 3; IL6R: interleukin 6 receptor; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; NSCLC: non-small cell lung cancer; RFP: red fluorescent protein; RPS6KB1/S6K: ribosomal protein S6 kinase B1; SQSTM1/p62: sequestosome 1; STAT3: signal transducer and activator of transcription 3; STK11: serine/threonine kinase 11.
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http://dx.doi.org/10.1080/15548627.2019.1634945DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138221PMC
April 2020

Vibration-Assisted Roll-Type Polishing System Based on Compliant Micro-Motion Stage.

Micromachines (Basel) 2018 Sep 29;9(10). Epub 2018 Sep 29.

Changchun Equipment and Technology Research Institute, Norinco Group, Changchun 130012, China.

This paper aims to create a high-quality surface based on the linear contact material removal mechanism. For this paper, a piezo-driven, flexure-based micro-motion stage was developed for the vibration-assisted roll-type precision polishing system. Meanwhile, the compliance matrix method was employed to establish the amplification ratio and compliance model of the flexure mechanism. The dimensions of the mechanism were optimized using the grey wolves optimization (GWO) algorithm, aiming to maximize the natural frequencies. Using the optimal parameters, the established models for the mechanical performance evaluation of the flexure stage were verified with the finite-element method. Through closed-loop test, it was proven that the proposed micro-motion stage performs well in positioning micro motions. Finally, high quality surface using silicon carbide (SiC) ceramic with 36 nm Sa was generated by the independently developed vibration-assisted roll-type polishing machine to validate the performance of the established polishing system.
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http://dx.doi.org/10.3390/mi9100499DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215217PMC
September 2018

Bone cement distribution is a potential predictor to the reconstructive effects of unilateral percutaneous kyphoplasty in OVCFs: a retrospective study.

J Orthop Surg Res 2018 Jun 7;13(1):140. Epub 2018 Jun 7.

Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pujian Road, Shanghai, 200127, China.

Background: Osteoporotic vertebral compression fracture (OVCF) is a common type of fracture, and percutaneous kyphoplasty (PKP) is an eligible solution to it. Previous studies have revealed that both the volume and filling pattern of bone cement correlate with the clinical outcomes after PKP procedure. However, the role of bone cement distribution remains to be illustrated.

Methods: To retrospectively evaluate the relationship between the bone cement distribution and the clinical outcomes of unilateral PKP, we enrolled 73 OVCF patients receiving unilateral PKP treatment. All the intervened vertebrae were classified into three groups based on the bone cement distribution observed on postoperative X-ray films. Preoperative and postoperative radiographic parameters including the vertebral height and kyphotic Cobb angle were recorded, and anterior vertebral height restoration rate (AVHRR) and Cobb angle correction (CR) were then calculated to assess the vertebral height reconstruction. Preoperative and postoperative Oswestry Disability Index (ODI) and visual analogue scale (VAS) were adopted by interviewing patients to assess the mobility improvement and pain relief. Demographic data, body mass index (BMI), lumbar bone mineral density (evaluated by BMD T-score) of each patient, bone cement volume (BV), and bone cement extravasation (BE) were also recorded. Between- and within-group comparisons and multivariable correlation analysis were carried out to analyze the data.

Results: VAS and ODI scores were both significantly improved in all of the enrolled cases with no significant differences between groups. Among the three groups, the average age, AVHRR, and BV were significantly different. Occurrence of BE was significantly different between two of the three groups. AVHRR was demonstrated to correlate negatively with preoperative anterior vertebral height ratio and positively with preoperative Cobb angle, CR, diffusion score, and ODI changes.

Conclusions: Bone cement distribution is a potential predictor to the reconstructive effects in unilateral PKP for OVCFs. Bone cement distribution is associated with AVHRR and BV, as well as the risk of BE occurrence. Greater bone cement distribution may indicate better vertebral restoration along with a higher BE risk.
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http://dx.doi.org/10.1186/s13018-018-0839-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992789PMC
June 2018

A pilot study of short T2* measurements with ultrashort echo time imaging at 0.35 T.

Biomed Eng Online 2018 Jun 4;17(1):70. Epub 2018 Jun 4.

Center for Biomedical Engineering, University of Science and Technology of China, Jinzhai Road, NO.96, Hefei, 230026, China.

Purpose: Ultrashort echo time (UTE) sequences play a key role in imaging and quantifying short T2 species. However, almost all of the relevant studies was conducted at relatively high fields. The purpose of this work was to further explore the feasibility of UTE imaging and T2* measurement for short T2 species at low fields.

Methods: A 2D UTE sequence with an echo time (TE) of 0.37 ms was developed on a 0.35 T permanent magnet scanner. This sequence acquires multiecho images to fit the monoexponential signal decay model for quantitative T2* calculations. In the phantom experiments, MnCl solutions with different T2* values were used to assess the curve fitting model in low fields. In the in vivo experiments, T2* measurements were performed on the Achilles tendon of five normal volunteers.

Results: The phantom studies showed a significant linear relationship between the MnCl solution concentration and R2* (1/T2*) values, which indicated the stability and accuracy of the T2* quantification model. The in vivo studies demonstrated that mean T2* value of Achilles tendon is 1.83 ± 0.21 ms, and the mean coefficient of determination (R-squared) was 0.996.

Conclusions: Both phantom and in vivo experiments showed that UTE imaging and quantification for short T2 components were feasible at low field 0.35 T scanner. This pilot study presents preliminary conclusions for future work.
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http://dx.doi.org/10.1186/s12938-018-0505-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987637PMC
June 2018

High throughput scaffold-based 3D micro-tumor array for efficient drug screening and chemosensitivity testing.

Biomaterials 2019 04 16;198:167-179. Epub 2018 May 16.

Department of Biomedical Engineering, School of Medicine, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Tsinghua University, Beijing, 100084, PR China. Electronic address:

Oncology drug development is greatly hampered by inefficient drug screening using 2D culture. Herein, we present ready-to-use micro-scaffolds in 384-well format to generate uniform 3D micro-tumor array (3D-MTA, CV < 0.15) that predicts in vivo drug responses more accurately than 2D monolayer. 3D-MTA generated from both cell lines and primary cells achieved high screen quality (Z' > 0.5), and were compatible with standard high throughput and high content instruments. Doxorubicin identified by 3D-MTA and 2D successfully inhibited tumor growth in mice bearing lung cancer cell line (H226) xenografts, but not gemcitabine and vinorelbine, which were selected solely by 2D. Resistance towards targeted therapy was modeled on 3D-MTA, which elicited SK-BR-3 to express higher proliferation-related genes in response to gefitinb, as compared to 2D. Screening of 56 MAPK inhibitors identified pisamertib to synergistically improve cytotoxicity effect in combination with gefitinib. Primary tumor cells derived from patient-derived xenografts further attested concordance of drug response in 3D-MTA with in vivo response. 3D-MTA was further extended to realize chemosensitivity testing using patient-derived cells. Overall, 3D-MTA demonstrated strong potential to accelerate drug discovery and improve cancer treatment by providing efficient drug screening.
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http://dx.doi.org/10.1016/j.biomaterials.2018.05.020DOI Listing
April 2019

Silencing of Long Noncoding RNA Triggers Cell Survival/Death Signaling via Oncogenes YBX1, MET, and p21 in Lung Cancer.

Cancer Res 2018 06 18;78(12):3207-3219. Epub 2018 Apr 18.

Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan.

The long noncoding RNA (lncRNA) has previously been identified as a prognostic marker in hepatocellular carcinoma. Here, we performed a comprehensive analysis of lncRNA expression profiles from RNA-Seq data and report that plays a similar role in lung cancer. Analysis of 918 lung cancer and normal lung tissues and lung cancer cell lines revealed that was significantly downregulated in lung cancer; this decreased expression was associated with poor patient survival. bound and stabilized the YBX1 protein. Silencing of triggered both cell survival and cell death signaling through dysregulation of the oncogenes YBX1, MET, and p21. In this network, p21 played an oncogenic role by promoting cell proliferation and antiapoptosis in lung cancers. played a tumor-suppressive role as indicated by inhibition of multiple cell cycle-related genes in human primary lung tumors. These data show that has potential as a new diagnostic and prognostic marker and as a therapeutic target for lung cancer. The lncRNA functions as a tumor suppressor, with potential use a diagnostic/prognostic marker and therapeutic target in lung cancer. .
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http://dx.doi.org/10.1158/0008-5472.CAN-18-0222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004254PMC
June 2018

Deep kernel learning method for SAR image target recognition.

Rev Sci Instrum 2017 Oct;88(10):104706

Automatic Test and Control Institute, Harbin Institute of Technology, Harbin 150080, China.

With the development of deep learning, research on image target recognition has made great progress in recent years. Remote sensing detection urgently requires target recognition for military, geographic, and other scientific research. This paper aims to solve the synthetic aperture radar image target recognition problem by combining deep and kernel learning. The model, which has a multilayer multiple kernel structure, is optimized layer by layer with the parameters of Support Vector Machine and a gradient descent algorithm. This new deep kernel learning method improves accuracy and achieves competitive recognition results compared with other learning methods.
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http://dx.doi.org/10.1063/1.4993064DOI Listing
October 2017

The identification of sub-centimetre nodules by near-infrared fluorescence thoracoscopic systems in pulmonary resection surgeries.

Eur J Cardiothorac Surg 2017 Dec;52(6):1190-1196

CAS Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing, China.

Objectives: Current surgical procedures lack high-sensitivity intraoperative imaging guidance, leading to undetected micro tumours. In vivo near-infrared (NIR) fluorescence imaging provides a powerful tool for identifying small nodules. The aim of this study was to examine our experience of using 2 different NIR devices in pulmonary resection surgery.

Methods: From August 2015 to October 2016, 36 patients with lung nodules underwent NIR fluorescence imaging thoracoscopic surgery. Two NIR devices: a D-Light P system and a SUPEREYE system were used. Patients were administered an injection of indocyanine green (ICG) through the peripheral vein 24 h preoperatively. During surgery, traditional white-light thoracoscopic exploration was performed first, followed by ICG-fluorescent-guided exploration. All detected nodules were resected and examined by a pathologist.

Results: Of the 36 patients, 76 nodules were resected. ICG-fluorescent imaging identified 68 nodules during in vivo exploration. The mean signal-to-background ratio of lung nodules in NIR exploration was 3.29 ± 1.81. The application of NIR devices led to the detection of 9 additional nodules that were missed using traditional detection methods (1 mm computed tomography scan and white-light thoracoscopic exploration) in 7 patients (19.4%). Four of the 9 nodules were confirmed as malignant or atypical adenomatous hyperplasia (44.4%). The other 5 nodules were confirmed as false-positive nodules. The sensitivities and positive predictive values of the ICG-fluorescent imaging for lung tumours were 88.7% and 92.6%, respectively.

Conclusions: This study demonstrated the feasibility and safety of using ICG-fluorescent imaging for multiple lung nodules identification in video-assisted thoracoscopic surgery pulmonary resection.

Clinicaltrial.gov Number: NCT02611245.
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http://dx.doi.org/10.1093/ejcts/ezx207DOI Listing
December 2017

Overexpression of LINC00152 correlates with poor patient survival and knockdown impairs cell proliferation in lung cancer.

Sci Rep 2017 06 7;7(1):2982. Epub 2017 Jun 7.

Section of Thoracic Surgery, University of Michigan, Ann Arbor, Michigan, USA.

We employed RNA sequencing analysis to reveal dysregulated lncRNAs in lung cancer utilizing 461 lung adenocarcinomas and 156 normal lung tissues from 3 separate cohorts. We found that LINC00152 was highly overexpressed in lung tumors as compared to their adjacent normal tissues. Patients with high LINC00152 expression demonstrate a significantly poorer survival than those with low expression. We verified the diagnostic/prognostic potential of LINC00152 expression in an independent cohort of lung tumor tissues using quantitative RT-PCR. After knockdown of LINC00152 using siRNAs in lung cancer cell lines, both cell proliferation and colony formation were decreased. Cell fractionation and qRT-PCR analysis indicated that LINC00152 is found mainly in the cytoplasm. Treatment with Trichostatin A in cell lines having low LINC00152 expression indicated that histone acetylation may be one mechanism underlying LINC00152 overexpression in NSCLC. Western blot analyses indicated that p38a, STAT1, STAT3, CREB1, CCNE1 and c-MYC proteins were decreased after LINC00152 siRNA treatment. Our study indicates LINC00152 plays an important role in lung tumor growth and is potentially a diagnostic/prognostic marker. Further characterization of LINC00152 in regulating its target proteins may provide a novel therapeutic target of lung cancer.
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http://dx.doi.org/10.1038/s41598-017-03043-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462773PMC
June 2017

Development and application of the near-infrared and white-light thoracoscope system for minimally invasive lung cancer surgery.

J Biomed Opt 2017 06;22(6):66002

Chinese Academy of Sciences, Institute of Automation, Key Laboratory of Molecular Imaging, Beijing, ChinabBeijing Key Laboratory of Molecular Imaging, Beijing, China.

In minimally invasive surgery, the white-light thoracoscope as a standard imaging tool is facing challenges of the low contrast between important anatomical or pathological regions and surrounding tissues. Recently, the near-infrared (NIR) fluorescence imaging shows superior advantages over the conventional white-light observation, which inspires researchers to develop imaging systems to improve overall outcomes of endoscopic imaging. We developed an NIR and white-light dual-channel thoracoscope system, which achieved high-fluorescent signal acquisition efficiency and the simultaneously optimal visualization of the NIR and color dual-channel signals. The system was designed to have fast and accurate image registration and high signal-to-background ratio by optimizing both software algorithms and optical hardware components for better performance in the NIR spectrum band. The system evaluation demonstrated that the minimally detectable concentration of indocyanine green (ICG) was 0.01 ?? ? M , and the spatial resolution was 35 ?? ? m . The in vivo feasibility of our system was verified by the preclinical experiments using six porcine models with the intravenous injection of ICG. Furthermore, the system was successfully applied for guiding the minimally invasive segmentectomy in three lung cancer patients, which revealed that our system held great promise for the clinical translation in lung cancer surgeries.
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http://dx.doi.org/10.1117/1.JBO.22.6.066002DOI Listing
June 2017

The Salutary Influence of Forest Bathing on Elderly Patients with Chronic Heart Failure.

Int J Environ Res Public Health 2017 03 31;14(4). Epub 2017 Mar 31.

Zhejiang Provincial Key Laboratory of Geriatrics & Geriatrics Institute of Zhejiang Province, Zhejiang Hospital, No.12 Lingyin Road, Hangzhou 310013, China.

The aim of the current study was to test the hypothesis that forest bathing would be beneficial for elderly patients with chronic heart failure (CHF) as an adjunctive therapy. Two groups of participants with CHF were simultaneously sent to the forest or an urban control area for a four-day trip, respectively. Subjects exposed to the forest site showed a significant reduction of brain natriuretic peptide (BNP) in comparison to that of the city group and their own baseline levels. The values for the cardiovascular disease related pathological factors, including endothelin-1 (ET-1), and constituents of the renin-angiotensin system (RAS), including renin, angiotensinogen (AGT), angiotensin II (ANGII), and ANGII receptor type 1 or 2 (AT1 or AT2) in subjects exposed to the forest environment were lower than those in the urban control group. Obviously, a decreased level of inflammatory cytokines and improved antioxidant function was observed in the forest group rather than in the city group. The assessment of the profile of mood states (POMS) indicated that the negative emotional mood state was alleviated after forest bathing. As anticipated, a better air quality in the forest site was observed according to the detection of PM (particulate matter <2.5 μm) and negative ions. These results provided direct evidence that forest bathing has a beneficial effect on CHF patients, and thus may pave the way for potential development of forest bathing as an effective adjunctive therapy on cardiovascular disorders.
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http://dx.doi.org/10.3390/ijerph14040368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5409569PMC
March 2017

Sublobar resection versus lobectomy in Surgical Treatment of Elderly Patients with early-stage non-small cell lung cancer (STEPS): study protocol for a randomized controlled trial.

Trials 2016 Apr 7;17:191. Epub 2016 Apr 7.

Department of Thoracic Surgery, Center for Mini-invasive Thoracic Surgery, People's Hospital, Peking University, #11 Xizhimen South Avenue, Beijing, 100044, China.

Background: The appropriateness of lobectomy for all elderly patients is controversial. Meanwhile, sublobar resection is associated with reduced operative risk, better preservation of pulmonary function, and a better quality of life, constituting a potential alternative to standard lobectomy for elderly patients with early-stage non-small cell lung cancer (NSCLC). To date, no randomized trial comparing sublobar resection and lobectomy focusing on elderly patients has been reported. We hypothesized that for patients at least 70 years old with clinical stage T1N0M0 NSCLC, sublobar resection is non-inferior to lobectomy for 3-year disease-free survival (DFS).

Methods/design: This is a prospective, randomized, controlled multicenter non-inferiority trial with two study arms: sublobar resection and lobectomy groups. Comprehensive geriatric assessments will be acquired for each patient. A total of 339 subjects will be enrolled on the basis of power calculations, and participants followed up every 6 months post-operation for 3 years. In case of relapse, survival follow-up will be continued until 5 years or death. Pulmonary function testing will be performed at 6, 12, and 36 months post-operation. The primary outcome is 3-year DFS; secondary endpoints include peri-operative complications and mortality, hospitalization time, post-operative ventilator time, overall survival, 3-year recurrence rates, post-operative pulmonary function, quality of life, geriatric assessment data, and 4-year mortality index.

Discussion: The present study is the only prospective, multicenter, randomized controlled trial comparing sublobar resection and lobectomy for elderly patients. The therapeutic outcomes of sublobar resection will be evaluated in comparison with lobectomy for elderly patients (≥70 years) with early-stage NSCLC.

Trial Registration Number: NCT02360761 : 01/24/2015 (ClinicalTrials.gov).
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http://dx.doi.org/10.1186/s13063-016-1312-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823889PMC
April 2016

The management of doornail lymph node in lobectomy.

J Vis Surg 2016 13;2. Epub 2016 Jan 13.

Department of Thoracic Surgery, People's Hospital of Peking University, Beijing 100044, China.

Background: The management of heavily adhesion caused by enlarged or calcified lymph nodes is long been one of the most common difficulties in lobectomy. Some of the lymph nodes, known as "the doornail lymph node", develop such massive adhesions that it significantly elevated the risk of uncontrollable bleeding and consequent conversion.

Methods: We performed a triple portal video-assisted thoracic surgery procedure. Lymph nodes were managed with coagulator, aspirator, scissors and suture. Arteries, veins and bronchus were ligated with stapler.

Results: One case of a 74-year-old female patient with multiple doornail lymph nodes was presented. An optimal technique to manage these lymph nodes was utilized: first, dissect the sheath of the blood vessel to find a bypass, second, remove the calcified core of the lymph node, and last, suture through the lymph node to ligate the adjacent artery. The surgery was successfully performed without complication.

Conclusions: The sequential technique of sheath dissection, core removal and suture ligation might be an optimal procedure in the management of doornail lymph node.
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http://dx.doi.org/10.3978/j.issn.2221-2965.2015.12.16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5637569PMC
January 2016